Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003365.01.T01 | XP_013468010.1 | 96.296 | 378 | 14 | 0 | 1 | 378 | 59 | 436 | 0 | 768 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003365.01.T01 | Q8S340 | 74.095 | 359 | 93 | 0 | 1 | 359 | 60 | 418 | 0 | 591 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003365.01.T01 | A0A072VJR0 | 96.296 | 378 | 14 | 0 | 1 | 378 | 59 | 436 | 0.0 | 768 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003365.01 | MsG0280009167.01 | 0.804336 | 2.268220e-49 | 1.166432e-46 |
| MsG0180003365.01 | MsG0780041397.01 | 0.816302 | 5.931882e-52 | 4.187828e-49 |
| MsG0180003365.01 | MsG0880043882.01 | 0.811355 | 7.297186e-51 | 4.506865e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780041115.01 | MsG0180003365.01 | PPI |
| MsG0380014132.01 | MsG0180003365.01 | PPI |
| MsG0380014132.01 | MsG0180003365.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003365.01.T01 | MTR_1g060360 | 96.296 | 378 | 14 | 0 | 1 | 378 | 59 | 436 | 0.0 | 768 |
| MsG0180003365.01.T01 | MTR_7g104330 | 76.720 | 378 | 88 | 0 | 1 | 378 | 67 | 444 | 0.0 | 642 |
| MsG0180003365.01.T01 | MTR_3g051240 | 65.193 | 362 | 125 | 1 | 1 | 362 | 56 | 416 | 0.0 | 509 |
| MsG0180003365.01.T01 | MTR_5g092360 | 63.830 | 376 | 130 | 2 | 1 | 376 | 62 | 431 | 1.41e-179 | 506 |
| MsG0180003365.01.T01 | MTR_7g104360 | 61.580 | 367 | 138 | 2 | 1 | 365 | 60 | 425 | 9.02e-173 | 488 |
| MsG0180003365.01.T01 | MTR_3g083810 | 40.632 | 443 | 170 | 10 | 3 | 356 | 87 | 525 | 2.69e-96 | 297 |
| MsG0180003365.01.T01 | MTR_0188s0020 | 42.440 | 377 | 194 | 6 | 3 | 356 | 78 | 454 | 2.31e-95 | 293 |
| MsG0180003365.01.T01 | MTR_3g083820 | 39.456 | 441 | 178 | 8 | 3 | 356 | 87 | 525 | 9.24e-95 | 293 |
| MsG0180003365.01.T01 | MTR_4g115670 | 42.781 | 374 | 192 | 6 | 3 | 354 | 72 | 445 | 9.85e-95 | 291 |
| MsG0180003365.01.T01 | MTR_6g013050 | 36.617 | 467 | 203 | 11 | 1 | 378 | 84 | 546 | 1.97e-93 | 291 |
| MsG0180003365.01.T01 | MTR_7g056403 | 37.868 | 441 | 185 | 7 | 3 | 356 | 86 | 524 | 6.43e-93 | 288 |
| MsG0180003365.01.T01 | MTR_4g037105 | 41.645 | 377 | 197 | 6 | 3 | 356 | 72 | 448 | 2.98e-91 | 282 |
| MsG0180003365.01.T01 | MTR_1g112630 | 41.270 | 378 | 198 | 8 | 3 | 356 | 73 | 450 | 2.25e-87 | 272 |
| MsG0180003365.01.T01 | MTR_6g013050 | 36.603 | 418 | 176 | 9 | 1 | 331 | 84 | 499 | 1.33e-82 | 262 |
| MsG0180003365.01.T01 | MTR_4g037670 | 28.398 | 412 | 216 | 13 | 1 | 353 | 231 | 622 | 3.07e-36 | 139 |
| MsG0180003365.01.T01 | MTR_1g052505 | 26.585 | 410 | 229 | 12 | 1 | 353 | 209 | 603 | 1.40e-33 | 132 |
| MsG0180003365.01.T01 | MTR_3g074930 | 26.521 | 411 | 232 | 15 | 1 | 349 | 194 | 596 | 4.11e-29 | 119 |
| MsG0180003365.01.T01 | MTR_3g074930 | 26.521 | 411 | 232 | 15 | 1 | 349 | 201 | 603 | 5.09e-29 | 119 |
| MsG0180003365.01.T01 | MTR_4g103520 | 26.161 | 409 | 236 | 14 | 1 | 349 | 121 | 523 | 3.51e-26 | 110 |
| MsG0180003365.01.T01 | MTR_4g103520 | 26.108 | 406 | 240 | 13 | 1 | 349 | 198 | 600 | 3.56e-26 | 110 |
| MsG0180003365.01.T01 | MTR_8g063080 | 26.355 | 406 | 239 | 14 | 1 | 349 | 183 | 585 | 3.67e-26 | 110 |
| MsG0180003365.01.T01 | MTR_5g045600 | 25.123 | 406 | 244 | 12 | 1 | 349 | 190 | 592 | 8.06e-26 | 109 |
| MsG0180003365.01.T01 | MTR_3g074970 | 25.750 | 400 | 204 | 14 | 1 | 349 | 143 | 500 | 1.74e-25 | 108 |
| MsG0180003365.01.T01 | MTR_4g082930 | 26.975 | 367 | 202 | 10 | 44 | 348 | 212 | 574 | 2.66e-23 | 102 |
| MsG0180003365.01.T01 | MTR_4g082940 | 31.047 | 277 | 142 | 11 | 115 | 348 | 290 | 560 | 3.84e-22 | 98.6 |
| MsG0180003365.01.T01 | MTR_5g092450 | 45.000 | 80 | 24 | 1 | 252 | 331 | 8 | 67 | 1.14e-13 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003365.01.T01 | AT3G52820 | 74.095 | 359 | 93 | 0 | 1 | 359 | 60 | 418 | 0.0 | 591 |
| MsG0180003365.01.T01 | AT3G52810 | 65.753 | 365 | 125 | 0 | 1 | 365 | 64 | 428 | 0.0 | 526 |
| MsG0180003365.01.T01 | AT3G20500 | 63.536 | 362 | 131 | 1 | 1 | 362 | 60 | 420 | 2.15e-174 | 493 |
| MsG0180003365.01.T01 | AT3G52780 | 61.351 | 370 | 137 | 4 | 1 | 365 | 57 | 425 | 1.43e-173 | 490 |
| MsG0180003365.01.T01 | AT3G52780 | 63.958 | 283 | 96 | 4 | 1 | 278 | 57 | 338 | 2.14e-133 | 386 |
| MsG0180003365.01.T01 | AT1G56360 | 44.531 | 384 | 185 | 9 | 1 | 356 | 65 | 448 | 2.31e-96 | 295 |
| MsG0180003365.01.T01 | AT4G36350 | 44.094 | 381 | 186 | 9 | 3 | 356 | 68 | 448 | 1.94e-95 | 293 |
| MsG0180003365.01.T01 | AT5G34850 | 41.388 | 389 | 200 | 6 | 3 | 363 | 71 | 459 | 4.64e-95 | 292 |
| MsG0180003365.01.T01 | AT3G07130 | 39.726 | 438 | 178 | 8 | 3 | 356 | 80 | 515 | 5.80e-95 | 293 |
| MsG0180003365.01.T01 | AT4G13700 | 43.681 | 364 | 151 | 7 | 1 | 312 | 78 | 439 | 1.40e-91 | 283 |
| MsG0180003365.01.T01 | AT2G18130 | 43.799 | 379 | 174 | 11 | 1 | 357 | 69 | 430 | 2.54e-90 | 279 |
| MsG0180003365.01.T01 | AT3G07130 | 36.943 | 471 | 178 | 9 | 3 | 356 | 80 | 548 | 5.10e-88 | 276 |
| MsG0180003365.01.T01 | AT2G27190 | 40.053 | 377 | 203 | 6 | 3 | 356 | 77 | 453 | 2.44e-86 | 270 |
| MsG0180003365.01.T01 | AT2G16430 | 40.053 | 377 | 203 | 6 | 3 | 356 | 76 | 452 | 1.83e-85 | 267 |
| MsG0180003365.01.T01 | AT2G32770 | 37.445 | 454 | 176 | 11 | 3 | 354 | 85 | 532 | 2.89e-77 | 248 |
| MsG0180003365.01.T01 | AT2G16430 | 40.979 | 327 | 168 | 7 | 54 | 356 | 7 | 332 | 4.35e-75 | 236 |
| MsG0180003365.01.T01 | AT4G13700 | 41.641 | 329 | 130 | 8 | 1 | 273 | 78 | 400 | 2.41e-71 | 229 |
| MsG0180003365.01.T01 | AT2G32770 | 42.157 | 306 | 124 | 5 | 41 | 299 | 65 | 364 | 1.28e-68 | 222 |
| MsG0180003365.01.T01 | AT3G46120 | 36.292 | 383 | 146 | 10 | 1 | 358 | 69 | 378 | 2.57e-63 | 207 |
| MsG0180003365.01.T01 | AT2G32770 | 33.921 | 454 | 163 | 12 | 3 | 354 | 85 | 503 | 5.27e-61 | 205 |
| MsG0180003365.01.T01 | AT1G52940 | 41.981 | 212 | 110 | 6 | 1 | 199 | 30 | 241 | 6.48e-39 | 139 |
| MsG0180003365.01.T01 | AT2G03450 | 31.408 | 277 | 138 | 10 | 115 | 348 | 304 | 571 | 4.42e-28 | 116 |
| MsG0180003365.01.T01 | AT1G13900 | 32.847 | 274 | 137 | 11 | 115 | 348 | 305 | 571 | 1.42e-27 | 114 |
| MsG0180003365.01.T01 | AT1G13750 | 25.743 | 404 | 236 | 13 | 1 | 345 | 184 | 582 | 1.50e-23 | 102 |
| MsG0180003365.01.T01 | AT5G50400 | 26.234 | 385 | 224 | 14 | 1 | 328 | 110 | 491 | 5.64e-23 | 100 |
| MsG0180003365.01.T01 | AT5G50400 | 26.234 | 385 | 224 | 14 | 1 | 328 | 182 | 563 | 6.40e-23 | 100 |
| MsG0180003365.01.T01 | AT4G24890 | 24.638 | 414 | 246 | 14 | 1 | 354 | 186 | 593 | 7.29e-22 | 97.8 |
Find 111 sgRNAs with CRISPR-Local
Find 137 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATCCATCATGTGGTAAT+TGG | 0.220374 | 1:+61227297 | MsG0180003365.01.T01:CDS |
| AAGGCCTGTGGTTGGATAAT+AGG | 0.249398 | 1:-61227844 | None:intergenic |
| CGTTATTTCTTGTATAAATC+AGG | 0.287616 | 1:+61227273 | MsG0180003365.01.T01:CDS |
| CATTTGTAGCTGACGATATA+TGG | 0.303483 | 1:+61228807 | MsG0180003365.01.T01:CDS |
| CAGACTCTGAACAGTATAAA+TGG | 0.311046 | 1:+61227992 | MsG0180003365.01.T01:CDS |
| TCAGAATCTTTGGTAGAGTA+TGG | 0.344560 | 1:+61227198 | MsG0180003365.01.T01:CDS |
| TTGGTAGAGTATGGAATAAA+AGG | 0.360287 | 1:+61227207 | MsG0180003365.01.T01:CDS |
| TAAGTAGTGCTTGGATTTAG+AGG | 0.362323 | 1:-61227321 | None:intergenic |
| GACTAGAATCTATGACAATA+AGG | 0.365814 | 1:+61228454 | MsG0180003365.01.T01:intron |
| TTGGGACTCATTCGGTCGTT+TGG | 0.366174 | 1:+61227756 | MsG0180003365.01.T01:CDS |
| CATGGAAGATTTGCTTTATG+AGG | 0.378875 | 1:+61228137 | MsG0180003365.01.T01:CDS |
| CCTATTGAATTTGTGGTTGT+GGG | 0.381585 | 1:+61227405 | MsG0180003365.01.T01:CDS |
| GCCTATTGAATTTGTGGTTG+TGG | 0.381694 | 1:+61227404 | MsG0180003365.01.T01:CDS |
| TTCTATAGATGTGGAGGATC+AGG | 0.385857 | 1:+61227345 | MsG0180003365.01.T01:CDS |
| CTGACCTAGCTAAGATTGAT+AGG | 0.394065 | 1:+61228022 | MsG0180003365.01.T01:CDS |
| CTATACAATGAGAGTGGACT+TGG | 0.396091 | 1:-61228698 | None:intergenic |
| AGGCCTGTGGTTGGATAATA+GGG | 0.405222 | 1:-61227843 | None:intergenic |
| TGAACGAAACACATGCAAAT+TGG | 0.408036 | 1:+61228756 | MsG0180003365.01.T01:CDS |
| CGATACCCATCAACCACTTT+GGG | 0.415060 | 1:+61227738 | MsG0180003365.01.T01:CDS |
| CAAGTTGCCTATTGAATTTG+TGG | 0.421879 | 1:+61227398 | MsG0180003365.01.T01:CDS |
| CCGATACCCATCAACCACTT+TGG | 0.422571 | 1:+61227737 | MsG0180003365.01.T01:CDS |
| AAGAATGTTGGGAGAGCTTA+GGG | 0.425144 | 1:+61228852 | MsG0180003365.01.T01:CDS |
| AGATCCATCATGTGGTAATT+GGG | 0.425516 | 1:+61227298 | MsG0180003365.01.T01:CDS |
| CGGTAACCATCCACGGCCTC+TGG | 0.438931 | 1:-61227795 | None:intergenic |
| CATGAGCCAACGAGCATTGT+AGG | 0.442128 | 1:-61227872 | None:intergenic |
| GGTAGAGCCACTCTCATTGT+AGG | 0.445927 | 1:-61227896 | None:intergenic |
| CTCAATCTCATGGTTGCCTT+CGG | 0.446049 | 1:-61227815 | None:intergenic |
| AAAGAATGTTGGGAGAGCTT+AGG | 0.449180 | 1:+61228851 | MsG0180003365.01.T01:CDS |
| GAGCAACACAAAAGAATGTT+GGG | 0.452158 | 1:+61228841 | MsG0180003365.01.T01:CDS |
| CAACCACTTTGGGACTCATT+CGG | 0.458686 | 1:+61227748 | MsG0180003365.01.T01:CDS |
| CCACGGCCTCTGGCTTGCGT+AGG | 0.463835 | 1:-61227785 | None:intergenic |
| ATGAGTCCCAAAGTGGTTGA+TGG | 0.464776 | 1:-61227744 | None:intergenic |
| CCTACGCAAGCCAGAGGCCG+TGG | 0.467453 | 1:+61227785 | MsG0180003365.01.T01:CDS |
| TACTTATATGTAGGGGACTT+GGG | 0.469043 | 1:+61227628 | MsG0180003365.01.T01:intron |
| TTGTATAGAGAGGCAAGCTT+TGG | 0.469280 | 1:+61228713 | MsG0180003365.01.T01:CDS |
| TTATGAGGCTCGTGTTGACT+TGG | 0.475526 | 1:+61228152 | MsG0180003365.01.T01:CDS |
| GGACTCATTCGGTCGTTTGG+TGG | 0.476264 | 1:+61227759 | MsG0180003365.01.T01:CDS |
| CTGATTTATACAAGAAATAA+CGG | 0.479402 | 1:-61227272 | None:intergenic |
| CCCACAACCACAAATTCAAT+AGG | 0.479971 | 1:-61227405 | None:intergenic |
| TGAGCAACACAAAAGAATGT+TGG | 0.492153 | 1:+61228840 | MsG0180003365.01.T01:CDS |
| TTACTTATATGTAGGGGACT+TGG | 0.492972 | 1:+61227627 | MsG0180003365.01.T01:intron |
| AAATGAGTAGTAGAGGTTTG+AGG | 0.495972 | 1:-61227917 | None:intergenic |
| AGCACTCATATCATCATGTT+GGG | 0.498108 | 1:+61227949 | MsG0180003365.01.T01:CDS |
| ATTCAATAGGCAACTTGAGT+GGG | 0.507898 | 1:-61227392 | None:intergenic |
| CACGGCCTCTGGCTTGCGTA+GGG | 0.508150 | 1:-61227784 | None:intergenic |
| TACAATGAGAGTGGACTTGG+AGG | 0.513147 | 1:-61228695 | None:intergenic |
| CCAAAGTGGTTGATGGGTAT+CGG | 0.515138 | 1:-61227737 | None:intergenic |
| AAGCACTCATATCATCATGT+TGG | 0.518546 | 1:+61227948 | MsG0180003365.01.T01:CDS |
| TCTTCCTATCAATCTTAGCT+AGG | 0.524738 | 1:-61228026 | None:intergenic |
| TGTAGGGGACTTGGGACAAA+CGG | 0.526077 | 1:+61227636 | MsG0180003365.01.T01:intron |
| CTATAGAAGTAAGTAGTGCT+TGG | 0.526754 | 1:-61227330 | None:intergenic |
| TCGGCATAAGATAGATCTCC+GGG | 0.527731 | 1:-61227718 | None:intergenic |
| CAACTTCAAATGAGTAGTAG+AGG | 0.533335 | 1:-61227924 | None:intergenic |
| GAGGCCCAATTACCACATGA+TGG | 0.534447 | 1:-61227302 | None:intergenic |
| GGTCCGTTGTACGTGACAAT+TGG | 0.541729 | 1:+61228488 | MsG0180003365.01.T01:CDS |
| ATACAAAGCGTTCGTATGCA+TGG | 0.542744 | 1:-61228191 | None:intergenic |
| TGATAGGAAGAGAACACCTT+GGG | 0.543516 | 1:+61228038 | MsG0180003365.01.T01:CDS |
| CGCAAGCCAGAGGCCGTGGA+TGG | 0.546137 | 1:+61227789 | MsG0180003365.01.T01:CDS |
| ATCGGCATAAGATAGATCTC+CGG | 0.555393 | 1:-61227719 | None:intergenic |
| CTCATAAAGCAAATCTTCCA+TGG | 0.557100 | 1:-61228136 | None:intergenic |
| AATTCAATAGGCAACTTGAG+TGG | 0.558815 | 1:-61227393 | None:intergenic |
| TGAGTCCCAAAGTGGTTGAT+GGG | 0.565645 | 1:-61227743 | None:intergenic |
| ACGAGCATTGTAGGATCTGA+AGG | 0.566265 | 1:-61227863 | None:intergenic |
| GTAGAGCCACTCTCATTGTA+GGG | 0.568561 | 1:-61227895 | None:intergenic |
| TAGGGATACTCTCAATCTCA+TGG | 0.571791 | 1:-61227825 | None:intergenic |
| AGATCATATGAGAGTGTCAT+GGG | 0.576396 | 1:+61227158 | None:intergenic |
| TGAAGGGGAAGATATGAGAC+AGG | 0.579980 | 1:+61228113 | MsG0180003365.01.T01:CDS |
| GGAAGATATGAGACAGGCCA+TGG | 0.580678 | 1:+61228119 | MsG0180003365.01.T01:CDS |
| GGGACTTGGGACAAACGGAA+TGG | 0.580838 | 1:+61227641 | MsG0180003365.01.T01:CDS |
| GTCACGTACAACGGACCACA+TGG | 0.583807 | 1:-61228482 | None:intergenic |
| AGGCAAGCTTTGGTCACGGA+AGG | 0.587551 | 1:+61228723 | MsG0180003365.01.T01:CDS |
| ACTTTAATGCAAGTCCCTCA+CGG | 0.591132 | 1:-61228523 | None:intergenic |
| TTTATACAAGAAATAACGGT+AGG | 0.597060 | 1:-61227268 | None:intergenic |
| TCCACTCTCATTGTATAGAG+AGG | 0.597080 | 1:+61228703 | MsG0180003365.01.T01:CDS |
| TTCAATAGGCAACTTGAGTG+GGG | 0.600517 | 1:-61227391 | None:intergenic |
| TTGCCTTCGGTAACCATCCA+CGG | 0.605693 | 1:-61227802 | None:intergenic |
| TTACCTTGTTGCATGCACCT+TGG | 0.606596 | 1:+61228064 | MsG0180003365.01.T01:CDS |
| ACGAGGCACATGAAGGTGAA+GGG | 0.607730 | 1:+61228097 | MsG0180003365.01.T01:CDS |
| GTAGAGTATGGAATAAAAGG+AGG | 0.610080 | 1:+61227210 | MsG0180003365.01.T01:CDS |
| AACGAGGCACATGAAGGTGA+AGG | 0.611380 | 1:+61228096 | MsG0180003365.01.T01:CDS |
| TATCCCTATTATCCAACCAC+AGG | 0.612100 | 1:+61227840 | MsG0180003365.01.T01:CDS |
| CGAGGCACATGAAGGTGAAG+GGG | 0.612934 | 1:+61228098 | MsG0180003365.01.T01:CDS |
| AAACACATGCAAATTGGTCA+TGG | 0.614401 | 1:+61228762 | MsG0180003365.01.T01:CDS |
| CGACCGAATGAGTCCCAAAG+TGG | 0.616021 | 1:-61227751 | None:intergenic |
| TCACCAATTGTCACGTACAA+CGG | 0.616161 | 1:-61228491 | None:intergenic |
| ACTACTTACTTCTATAGATG+TGG | 0.616768 | 1:+61227336 | MsG0180003365.01.T01:CDS |
| ATACAAGAAATAACGGTAGG+AGG | 0.617230 | 1:-61227265 | None:intergenic |
| AAGATCATATGAGAGTGTCA+TGG | 0.618004 | 1:+61227157 | None:intergenic |
| GACAATAAGGCTGATCCATG+TGG | 0.619932 | 1:+61228467 | MsG0180003365.01.T01:CDS |
| CTCATGCCCTACAATGAGAG+TGG | 0.625191 | 1:+61227889 | MsG0180003365.01.T01:CDS |
| TCAGATCCTACAATGCTCGT+TGG | 0.626158 | 1:+61227866 | MsG0180003365.01.T01:CDS |
| TTGTACGTGACAATTGGTGA+TGG | 0.631240 | 1:+61228494 | MsG0180003365.01.T01:CDS |
| TGCAACAAGGTAATCACCCA+AGG | 0.633296 | 1:-61228054 | None:intergenic |
| ACTTACTTCTATAGATGTGG+AGG | 0.633495 | 1:+61227339 | MsG0180003365.01.T01:CDS |
| AGGATCTGAAGGCCTGTGGT+TGG | 0.633708 | 1:-61227852 | None:intergenic |
| AATACTAACGAGGCACATGA+AGG | 0.634056 | 1:+61228090 | MsG0180003365.01.T01:CDS |
| GCCTCTCTATACAATGAGAG+TGG | 0.636450 | 1:-61228704 | None:intergenic |
| TTGATAGGAAGAGAACACCT+TGG | 0.647234 | 1:+61228037 | MsG0180003365.01.T01:CDS |
| ATCAGGCAAGATCCATCATG+TGG | 0.651389 | 1:+61227290 | MsG0180003365.01.T01:CDS |
| TGGAACCCTACGCAAGCCAG+AGG | 0.652554 | 1:+61227779 | MsG0180003365.01.T01:CDS |
| AGAGAGGCAAGCTTTGGTCA+CGG | 0.654681 | 1:+61228719 | MsG0180003365.01.T01:CDS |
| GGTGTCTTGAAAGAAAACTC+AGG | 0.656796 | 1:-61227369 | None:intergenic |
| AGGCCGTGGATGGTTACCGA+AGG | 0.658332 | 1:+61227799 | MsG0180003365.01.T01:CDS |
| TCAATAGGCAACTTGAGTGG+GGG | 0.673793 | 1:-61227390 | None:intergenic |
| TACGTGACAATTGGTGATGG+AGG | 0.674382 | 1:+61228497 | MsG0180003365.01.T01:CDS |
| GGTGATGGAGGAAACCGTGA+GGG | 0.679946 | 1:+61228509 | MsG0180003365.01.T01:CDS |
| ATACCAAGGTGCATGCAACA+AGG | 0.682936 | 1:-61228067 | None:intergenic |
| GCCTCGTTAGTATTATACCA+AGG | 0.707400 | 1:-61228081 | None:intergenic |
| TTGTAGGATCTGAAGGCCTG+TGG | 0.708691 | 1:-61227856 | None:intergenic |
| TGGTGATGGAGGAAACCGTG+AGG | 0.719884 | 1:+61228508 | MsG0180003365.01.T01:CDS |
| ACCTTGGTATAATACTAACG+AGG | 0.774313 | 1:+61228080 | MsG0180003365.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAATAAAAATACATGT+AGG | - | Chr1:61228608-61228627 | None:intergenic | 10.0% |
| !! | AAATTACTTACTTATATGTA+GGG | + | Chr1:61227620-61227639 | MsG0180003365.01.T01:intron | 15.0% |
| !! | TAAAATAAACTTCATCATTA+AGG | - | Chr1:61228559-61228578 | None:intergenic | 15.0% |
| !! | TAAATTACTTACTTATATGT+AGG | + | Chr1:61227619-61227638 | MsG0180003365.01.T01:intron | 15.0% |
| !! | TACTAGAAAAATTGAAATAA+CGG | + | Chr1:61228246-61228265 | MsG0180003365.01.T01:intron | 15.0% |
| !! | TTTAAAAATAATAAGTGAGA+TGG | - | Chr1:61228406-61228425 | None:intergenic | 15.0% |
| !! | AAATAAAAATACATGTAGGT+AGG | - | Chr1:61228604-61228623 | None:intergenic | 20.0% |
| !! | AAATTGTAGAAAAATGAAAG+AGG | - | Chr1:61227564-61227583 | None:intergenic | 20.0% |
| !! | AAGAGATGTAAAAATATAAG+TGG | - | Chr1:61227515-61227534 | None:intergenic | 20.0% |
| !! | AATATAGCAAAAAAGCAATT+GGG | + | Chr1:61227235-61227254 | MsG0180003365.01.T01:CDS | 20.0% |
| !! | AATTACTTACTTATATGTAG+GGG | + | Chr1:61227621-61227640 | MsG0180003365.01.T01:intron | 20.0% |
| !! | AGAAAATTTAGAAACACAAA+AGG | + | Chr1:61228269-61228288 | MsG0180003365.01.T01:intron | 20.0% |
| !! | AGAGATGTAAAAATATAAGT+GGG | - | Chr1:61227514-61227533 | None:intergenic | 20.0% |
| !! | CTGATTTATACAAGAAATAA+CGG | - | Chr1:61227275-61227294 | None:intergenic | 20.0% |
| ! | AATTTCGTCCTAAATATTAG+AGG | + | Chr1:61228307-61228326 | MsG0180003365.01.T01:intron | 25.0% |
| ! | ATATAGCAAAAAAGCAATTG+GGG | + | Chr1:61227236-61227255 | MsG0180003365.01.T01:CDS | 25.0% |
| ! | GAATATAGCAAAAAAGCAAT+TGG | + | Chr1:61227234-61227253 | MsG0180003365.01.T01:CDS | 25.0% |
| ! | TTTATACAAGAAATAACGGT+AGG | - | Chr1:61227271-61227290 | None:intergenic | 25.0% |
| !! | ATTTAGCTCCTCTAATATTT+AGG | - | Chr1:61228318-61228337 | None:intergenic | 25.0% |
| !!! | CGTTATTTCTTGTATAAATC+AGG | + | Chr1:61227273-61227292 | MsG0180003365.01.T01:CDS | 25.0% |
| !!! | TTTGCTATCAATGTGTTTTA+AGG | - | Chr1:61227677-61227696 | None:intergenic | 25.0% |
| ACTACTTACTTCTATAGATG+TGG | + | Chr1:61227336-61227355 | MsG0180003365.01.T01:CDS | 30.0% | |
| AGAAAAAGACTCAGAATCTT+TGG | + | Chr1:61227188-61227207 | MsG0180003365.01.T01:CDS | 30.0% | |
| GACTAGAATCTATGACAATA+AGG | + | Chr1:61228454-61228473 | MsG0180003365.01.T01:intron | 30.0% | |
| TTGGTAGAGTATGGAATAAA+AGG | + | Chr1:61227207-61227226 | MsG0180003365.01.T01:CDS | 30.0% | |
| ! | AAATGAAAGAGGTATAAGTG+AGG | - | Chr1:61227553-61227572 | None:intergenic | 30.0% |
| ! | TGTTGATTGTTGGATTGTAT+AGG | + | Chr1:61228663-61228682 | MsG0180003365.01.T01:intron | 30.0% |
| AAACACATGCAAATTGGTCA+TGG | + | Chr1:61228762-61228781 | MsG0180003365.01.T01:CDS | 35.0% | |
| AAATGAGTAGTAGAGGTTTG+AGG | - | Chr1:61227920-61227939 | None:intergenic | 35.0% | |
| AAGATCCATCATGTGGTAAT+TGG | + | Chr1:61227297-61227316 | MsG0180003365.01.T01:CDS | 35.0% | |
| AAGCACTCATATCATCATGT+TGG | + | Chr1:61227948-61227967 | MsG0180003365.01.T01:CDS | 35.0% | |
| AATTCAATAGGCAACTTGAG+TGG | - | Chr1:61227396-61227415 | None:intergenic | 35.0% | |
| ACCTTGGTATAATACTAACG+AGG | + | Chr1:61228080-61228099 | MsG0180003365.01.T01:CDS | 35.0% | |
| ACTTACTTCTATAGATGTGG+AGG | + | Chr1:61227339-61227358 | MsG0180003365.01.T01:CDS | 35.0% | |
| AGATCCATCATGTGGTAATT+GGG | + | Chr1:61227298-61227317 | MsG0180003365.01.T01:CDS | 35.0% | |
| AGCACTCATATCATCATGTT+GGG | + | Chr1:61227949-61227968 | MsG0180003365.01.T01:CDS | 35.0% | |
| ATACAAGAAATAACGGTAGG+AGG | - | Chr1:61227268-61227287 | None:intergenic | 35.0% | |
| ATTCAATAGGCAACTTGAGT+GGG | - | Chr1:61227395-61227414 | None:intergenic | 35.0% | |
| CAACTTCAAATGAGTAGTAG+AGG | - | Chr1:61227927-61227946 | None:intergenic | 35.0% | |
| CAAGTTGCCTATTGAATTTG+TGG | + | Chr1:61227398-61227417 | MsG0180003365.01.T01:CDS | 35.0% | |
| CAGACTCTGAACAGTATAAA+TGG | + | Chr1:61227992-61228011 | MsG0180003365.01.T01:CDS | 35.0% | |
| CATTTGTAGCTGACGATATA+TGG | + | Chr1:61228807-61228826 | MsG0180003365.01.T01:CDS | 35.0% | |
| CCTATTGAATTTGTGGTTGT+GGG | + | Chr1:61227405-61227424 | MsG0180003365.01.T01:CDS | 35.0% | |
| CTATAGAAGTAAGTAGTGCT+TGG | - | Chr1:61227333-61227352 | None:intergenic | 35.0% | |
| CTCATAAAGCAAATCTTCCA+TGG | - | Chr1:61228139-61228158 | None:intergenic | 35.0% | |
| GAGCAACACAAAAGAATGTT+GGG | + | Chr1:61228841-61228860 | MsG0180003365.01.T01:CDS | 35.0% | |
| GTAGAGTATGGAATAAAAGG+AGG | + | Chr1:61227210-61227229 | MsG0180003365.01.T01:CDS | 35.0% | |
| TAAGTAGTGCTTGGATTTAG+AGG | - | Chr1:61227324-61227343 | None:intergenic | 35.0% | |
| TACTTATATGTAGGGGACTT+GGG | + | Chr1:61227628-61227647 | MsG0180003365.01.T01:intron | 35.0% | |
| TCAGAATCTTTGGTAGAGTA+TGG | + | Chr1:61227198-61227217 | MsG0180003365.01.T01:CDS | 35.0% | |
| TCATAGATTCTAGTCTGCAA+GGG | - | Chr1:61228449-61228468 | None:intergenic | 35.0% | |
| TCTTCCTATCAATCTTAGCT+AGG | - | Chr1:61228029-61228048 | None:intergenic | 35.0% | |
| TGAACGAAACACATGCAAAT+TGG | + | Chr1:61228756-61228775 | MsG0180003365.01.T01:CDS | 35.0% | |
| TGAGCAACACAAAAGAATGT+TGG | + | Chr1:61228840-61228859 | MsG0180003365.01.T01:CDS | 35.0% | |
| TTACTTATATGTAGGGGACT+TGG | + | Chr1:61227627-61227646 | MsG0180003365.01.T01:intron | 35.0% | |
| ! | CACATGTACATGTTGATTGT+TGG | + | Chr1:61228653-61228672 | MsG0180003365.01.T01:intron | 35.0% |
| ! | CATGGAAGATTTGCTTTATG+AGG | + | Chr1:61228137-61228156 | MsG0180003365.01.T01:CDS | 35.0% |
| !!! | TCGTGTTGACTTGGTTTTTT+CGG | + | Chr1:61228161-61228180 | MsG0180003365.01.T01:CDS | 35.0% |
| AAAGAATGTTGGGAGAGCTT+AGG | + | Chr1:61228851-61228870 | MsG0180003365.01.T01:CDS | 40.0% | |
| AAGAATGTTGGGAGAGCTTA+GGG | + | Chr1:61228852-61228871 | MsG0180003365.01.T01:CDS | 40.0% | |
| AATACTAACGAGGCACATGA+AGG | + | Chr1:61228090-61228109 | MsG0180003365.01.T01:CDS | 40.0% | |
| ACTTTAATGCAAGTCCCTCA+CGG | - | Chr1:61228526-61228545 | None:intergenic | 40.0% | |
| ATCGGCATAAGATAGATCTC+CGG | - | Chr1:61227722-61227741 | None:intergenic | 40.0% | |
| CCCACAACCACAAATTCAAT+AGG | - | Chr1:61227408-61227427 | None:intergenic | 40.0% | |
| CTGACCTAGCTAAGATTGAT+AGG | + | Chr1:61228022-61228041 | MsG0180003365.01.T01:CDS | 40.0% | |
| GCCTATTGAATTTGTGGTTG+TGG | + | Chr1:61227404-61227423 | MsG0180003365.01.T01:CDS | 40.0% | |
| GCCTCGTTAGTATTATACCA+AGG | - | Chr1:61228084-61228103 | None:intergenic | 40.0% | |
| GGTGTCTTGAAAGAAAACTC+AGG | - | Chr1:61227372-61227391 | None:intergenic | 40.0% | |
| GTCATAGATTCTAGTCTGCA+AGG | - | Chr1:61228450-61228469 | None:intergenic | 40.0% | |
| TAGGGATACTCTCAATCTCA+TGG | - | Chr1:61227828-61227847 | None:intergenic | 40.0% | |
| TATCCCTATTATCCAACCAC+AGG | + | Chr1:61227840-61227859 | MsG0180003365.01.T01:CDS | 40.0% | |
| TCACCAATTGTCACGTACAA+CGG | - | Chr1:61228494-61228513 | None:intergenic | 40.0% | |
| TCCACTCTCATTGTATAGAG+AGG | + | Chr1:61228703-61228722 | MsG0180003365.01.T01:CDS | 40.0% | |
| TGATAGGAAGAGAACACCTT+GGG | + | Chr1:61228038-61228057 | MsG0180003365.01.T01:CDS | 40.0% | |
| TTCAATAGGCAACTTGAGTG+GGG | - | Chr1:61227394-61227413 | None:intergenic | 40.0% | |
| TTCTATAGATGTGGAGGATC+AGG | + | Chr1:61227345-61227364 | MsG0180003365.01.T01:CDS | 40.0% | |
| TTGATAGGAAGAGAACACCT+TGG | + | Chr1:61228037-61228056 | MsG0180003365.01.T01:CDS | 40.0% | |
| TTGTACGTGACAATTGGTGA+TGG | + | Chr1:61228494-61228513 | MsG0180003365.01.T01:CDS | 40.0% | |
| TTGTATAGAGAGGCAAGCTT+TGG | + | Chr1:61228713-61228732 | MsG0180003365.01.T01:CDS | 40.0% | |
| ! | ATACAAAGCGTTCGTATGCA+TGG | - | Chr1:61228194-61228213 | None:intergenic | 40.0% |
| ! | CTATACAATGAGAGTGGACT+TGG | - | Chr1:61228701-61228720 | None:intergenic | 40.0% |
| !! | TACGACGTGTTTTTGTTACC+CGG | + | Chr1:61227700-61227719 | MsG0180003365.01.T01:CDS | 40.0% |
| !!! | CGTGTTGACTTGGTTTTTTC+GGG | + | Chr1:61228162-61228181 | MsG0180003365.01.T01:CDS | 40.0% |
| !!! | GTGTTGACTTGGTTTTTTCG+GGG | + | Chr1:61228163-61228182 | MsG0180003365.01.T01:CDS | 40.0% |
| AAGGCCTGTGGTTGGATAAT+AGG | - | Chr1:61227847-61227866 | None:intergenic | 45.0% | |
| AGGCCTGTGGTTGGATAATA+GGG | - | Chr1:61227846-61227865 | None:intergenic | 45.0% | |
| ATACCAAGGTGCATGCAACA+AGG | - | Chr1:61228070-61228089 | None:intergenic | 45.0% | |
| ATCAGGCAAGATCCATCATG+TGG | + | Chr1:61227290-61227309 | MsG0180003365.01.T01:CDS | 45.0% | |
| ATGAGTCCCAAAGTGGTTGA+TGG | - | Chr1:61227747-61227766 | None:intergenic | 45.0% | |
| CGATACCCATCAACCACTTT+GGG | + | Chr1:61227738-61227757 | MsG0180003365.01.T01:CDS | 45.0% | |
| CTCAATCTCATGGTTGCCTT+CGG | - | Chr1:61227818-61227837 | None:intergenic | 45.0% | |
| GACAATAAGGCTGATCCATG+TGG | + | Chr1:61228467-61228486 | MsG0180003365.01.T01:CDS | 45.0% | |
| GCCTCTCTATACAATGAGAG+TGG | - | Chr1:61228707-61228726 | None:intergenic | 45.0% | |
| GTAGAGCCACTCTCATTGTA+GGG | - | Chr1:61227898-61227917 | None:intergenic | 45.0% | |
| TCAATAGGCAACTTGAGTGG+GGG | - | Chr1:61227393-61227412 | None:intergenic | 45.0% | |
| TCAGATCCTACAATGCTCGT+TGG | + | Chr1:61227866-61227885 | MsG0180003365.01.T01:CDS | 45.0% | |
| TCGGCATAAGATAGATCTCC+GGG | - | Chr1:61227721-61227740 | None:intergenic | 45.0% | |
| TGAAGGGGAAGATATGAGAC+AGG | + | Chr1:61228113-61228132 | MsG0180003365.01.T01:CDS | 45.0% | |
| TGAGTCCCAAAGTGGTTGAT+GGG | - | Chr1:61227746-61227765 | None:intergenic | 45.0% | |
| TGCAACAAGGTAATCACCCA+AGG | - | Chr1:61228057-61228076 | None:intergenic | 45.0% | |
| TTACCTTGTTGCATGCACCT+TGG | + | Chr1:61228064-61228083 | MsG0180003365.01.T01:CDS | 45.0% | |
| ! | ACGAGCATTGTAGGATCTGA+AGG | - | Chr1:61227866-61227885 | None:intergenic | 45.0% |
| ! | TACAATGAGAGTGGACTTGG+AGG | - | Chr1:61228698-61228717 | None:intergenic | 45.0% |
| !! | CAACCACTTTGGGACTCATT+CGG | + | Chr1:61227748-61227767 | MsG0180003365.01.T01:CDS | 45.0% |
| !! | CCAAAGTGGTTGATGGGTAT+CGG | - | Chr1:61227740-61227759 | None:intergenic | 45.0% |
| !! | TACGTGACAATTGGTGATGG+AGG | + | Chr1:61228497-61228516 | MsG0180003365.01.T01:CDS | 45.0% |
| !! | TTATGAGGCTCGTGTTGACT+TGG | + | Chr1:61228152-61228171 | MsG0180003365.01.T01:CDS | 45.0% |
| AACGAGGCACATGAAGGTGA+AGG | + | Chr1:61228096-61228115 | MsG0180003365.01.T01:CDS | 50.0% | |
| ACGAGGCACATGAAGGTGAA+GGG | + | Chr1:61228097-61228116 | MsG0180003365.01.T01:CDS | 50.0% | |
| CCGATACCCATCAACCACTT+TGG | + | Chr1:61227737-61227756 | MsG0180003365.01.T01:CDS | 50.0% | |
| CTCATGCCCTACAATGAGAG+TGG | + | Chr1:61227889-61227908 | MsG0180003365.01.T01:CDS | 50.0% | |
| GAGGCCCAATTACCACATGA+TGG | - | Chr1:61227305-61227324 | None:intergenic | 50.0% | |
| GGAAGATATGAGACAGGCCA+TGG | + | Chr1:61228119-61228138 | MsG0180003365.01.T01:CDS | 50.0% | |
| GGTAGAGCCACTCTCATTGT+AGG | - | Chr1:61227899-61227918 | None:intergenic | 50.0% | |
| GGTCCGTTGTACGTGACAAT+TGG | + | Chr1:61228488-61228507 | MsG0180003365.01.T01:CDS | 50.0% | |
| TGTAGGGGACTTGGGACAAA+CGG | + | Chr1:61227636-61227655 | MsG0180003365.01.T01:intron | 50.0% | |
| TTGCCTTCGGTAACCATCCA+CGG | - | Chr1:61227805-61227824 | None:intergenic | 50.0% | |
| TTGGGACTCATTCGGTCGTT+TGG | + | Chr1:61227756-61227775 | MsG0180003365.01.T01:CDS | 50.0% | |
| TTGTAGGATCTGAAGGCCTG+TGG | - | Chr1:61227859-61227878 | None:intergenic | 50.0% | |
| ! | AGAGAGGCAAGCTTTGGTCA+CGG | + | Chr1:61228719-61228738 | MsG0180003365.01.T01:CDS | 50.0% |
| ! | CATGAGCCAACGAGCATTGT+AGG | - | Chr1:61227875-61227894 | None:intergenic | 50.0% |
| AGGATCTGAAGGCCTGTGGT+TGG | - | Chr1:61227855-61227874 | None:intergenic | 55.0% | |
| CGACCGAATGAGTCCCAAAG+TGG | - | Chr1:61227754-61227773 | None:intergenic | 55.0% | |
| CGAGGCACATGAAGGTGAAG+GGG | + | Chr1:61228098-61228117 | MsG0180003365.01.T01:CDS | 55.0% | |
| GGACTCATTCGGTCGTTTGG+TGG | + | Chr1:61227759-61227778 | MsG0180003365.01.T01:CDS | 55.0% | |
| GGGACTTGGGACAAACGGAA+TGG | + | Chr1:61227641-61227660 | MsG0180003365.01.T01:CDS | 55.0% | |
| GTCACGTACAACGGACCACA+TGG | - | Chr1:61228485-61228504 | None:intergenic | 55.0% | |
| ! | AGGCAAGCTTTGGTCACGGA+AGG | + | Chr1:61228723-61228742 | MsG0180003365.01.T01:CDS | 55.0% |
| !! | GGTGATGGAGGAAACCGTGA+GGG | + | Chr1:61228509-61228528 | MsG0180003365.01.T01:CDS | 55.0% |
| !! | TGGTGATGGAGGAAACCGTG+AGG | + | Chr1:61228508-61228527 | MsG0180003365.01.T01:CDS | 55.0% |
| AGGCCGTGGATGGTTACCGA+AGG | + | Chr1:61227799-61227818 | MsG0180003365.01.T01:CDS | 60.0% | |
| TGGAACCCTACGCAAGCCAG+AGG | + | Chr1:61227779-61227798 | MsG0180003365.01.T01:CDS | 60.0% | |
| CGGTAACCATCCACGGCCTC+TGG | - | Chr1:61227798-61227817 | None:intergenic | 65.0% | |
| ! | CACGGCCTCTGGCTTGCGTA+GGG | - | Chr1:61227787-61227806 | None:intergenic | 65.0% |
| CCTACGCAAGCCAGAGGCCG+TGG | + | Chr1:61227785-61227804 | MsG0180003365.01.T01:CDS | 70.0% | |
| CGCAAGCCAGAGGCCGTGGA+TGG | + | Chr1:61227789-61227808 | MsG0180003365.01.T01:CDS | 70.0% | |
| ! | CCACGGCCTCTGGCTTGCGT+AGG | - | Chr1:61227788-61227807 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 61227165 | 61228901 | 61227165 | ID=MsG0180003365.01;Name=MsG0180003365.01 |
| Chr1 | mRNA | 61227165 | 61228901 | 61227165 | ID=MsG0180003365.01.T01;Parent=MsG0180003365.01;Name=MsG0180003365.01.T01;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|1|1|4|0|378 |
| Chr1 | exon | 61227165 | 61227426 | 61227165 | ID=MsG0180003365.01.T01:exon:4209;Parent=MsG0180003365.01.T01 |
| Chr1 | exon | 61227641 | 61228209 | 61227641 | ID=MsG0180003365.01.T01:exon:4210;Parent=MsG0180003365.01.T01 |
| Chr1 | exon | 61228455 | 61228543 | 61228455 | ID=MsG0180003365.01.T01:exon:4211;Parent=MsG0180003365.01.T01 |
| Chr1 | exon | 61228685 | 61228901 | 61228685 | ID=MsG0180003365.01.T01:exon:4212;Parent=MsG0180003365.01.T01 |
| Chr1 | CDS | 61227165 | 61227426 | 61227165 | ID=MsG0180003365.01.T01:cds;Parent=MsG0180003365.01.T01 |
| Chr1 | CDS | 61227641 | 61228209 | 61227641 | ID=MsG0180003365.01.T01:cds;Parent=MsG0180003365.01.T01 |
| Chr1 | CDS | 61228455 | 61228543 | 61228455 | ID=MsG0180003365.01.T01:cds;Parent=MsG0180003365.01.T01 |
| Chr1 | CDS | 61228685 | 61228901 | 61228685 | ID=MsG0180003365.01.T01:cds;Parent=MsG0180003365.01.T01 |
| Gene Sequence |
| Protein sequence |
