Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003807.01.T01 | KAF7818443.1 | 73.259 | 1077 | 205 | 17 | 72 | 1111 | 5 | 1035 | 0 | 1555 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003807.01.T01 | A0A834TAA1 | 73.259 | 1077 | 205 | 17 | 72 | 1111 | 5 | 1035 | 0.0 | 1555 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047958.01 | MsG0180003807.01 | 0.816861 | 4.445507e-52 | 3.186701e-49 |
| MsG0080048058.01 | MsG0180003807.01 | 0.856431 | 3.233009e-62 | 7.715544e-59 |
| MsG0080048461.01 | MsG0180003807.01 | 0.809608 | 1.739504e-50 | 1.026015e-47 |
| MsG0080048787.01 | MsG0180003807.01 | 0.827343 | 1.648350e-54 | 1.587138e-51 |
| MsG0080048928.01 | MsG0180003807.01 | 0.817872 | 2.631603e-52 | 1.939437e-49 |
| MsG0080049081.01 | MsG0180003807.01 | 0.816814 | 4.553602e-52 | 3.259986e-49 |
| MsG0180000001.01 | MsG0180003807.01 | 0.814871 | 1.235161e-51 | 8.384881e-49 |
| MsG0180000065.01 | MsG0180003807.01 | 0.808454 | 3.072472e-50 | 1.757591e-47 |
| MsG0180000091.01 | MsG0180003807.01 | 0.826957 | 2.039365e-54 | 1.941393e-51 |
| MsG0180000100.01 | MsG0180003807.01 | 0.835686 | 1.452106e-56 | 1.789082e-53 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003807.01.T01 | MTR_1g069735 | 96.986 | 564 | 4 | 1 | 74 | 637 | 1 | 551 | 0.0 | 1131 |
| MsG0180003807.01.T01 | MTR_1g069735 | 98.755 | 482 | 6 | 0 | 74 | 555 | 1 | 482 | 0.0 | 1000 |
| MsG0180003807.01.T01 | MTR_1g069735 | 98.932 | 468 | 5 | 0 | 74 | 541 | 1 | 468 | 0.0 | 971 |
| MsG0180003807.01.T01 | MTR_1g069725 | 98.504 | 468 | 7 | 0 | 648 | 1115 | 1 | 468 | 0.0 | 969 |
| MsG0180003807.01.T01 | MTR_7g097150 | 81.884 | 552 | 80 | 4 | 74 | 621 | 1 | 536 | 0.0 | 942 |
| MsG0180003807.01.T01 | MTR_1g075880 | 77.009 | 535 | 106 | 4 | 74 | 607 | 1 | 519 | 0.0 | 847 |
| MsG0180003807.01.T01 | MTR_1g442790 | 72.105 | 570 | 124 | 8 | 74 | 640 | 1 | 538 | 0.0 | 800 |
| MsG0180003807.01.T01 | MTR_1g442790 | 75.047 | 529 | 106 | 6 | 74 | 600 | 1 | 505 | 0.0 | 782 |
| MsG0180003807.01.T01 | MTR_1g042700 | 69.418 | 533 | 141 | 6 | 74 | 604 | 1 | 513 | 0.0 | 746 |
| MsG0180003807.01.T01 | MTR_3g104700 | 66.667 | 468 | 135 | 2 | 117 | 575 | 18 | 473 | 0.0 | 655 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003807.01.T01 | AT2G20470 | 73.739 | 575 | 122 | 6 | 74 | 635 | 1 | 559 | 0.0 | 842 |
| MsG0180003807.01.T01 | AT2G20470 | 73.739 | 575 | 122 | 6 | 74 | 635 | 1 | 559 | 0.0 | 842 |
| MsG0180003807.01.T01 | AT4G14350 | 74.555 | 562 | 118 | 4 | 74 | 634 | 1 | 538 | 0.0 | 839 |
| MsG0180003807.01.T01 | AT4G14350 | 74.555 | 562 | 118 | 4 | 74 | 634 | 1 | 538 | 0.0 | 839 |
| MsG0180003807.01.T01 | AT4G14350 | 74.555 | 562 | 118 | 4 | 74 | 634 | 1 | 538 | 0.0 | 839 |
| MsG0180003807.01.T01 | AT4G14350 | 74.555 | 562 | 118 | 4 | 74 | 634 | 1 | 538 | 0.0 | 839 |
| MsG0180003807.01.T01 | AT2G20470 | 76.636 | 535 | 104 | 5 | 74 | 603 | 1 | 519 | 0.0 | 833 |
| MsG0180003807.01.T01 | AT3G23310 | 72.887 | 568 | 128 | 4 | 74 | 638 | 1 | 545 | 0.0 | 832 |
| MsG0180003807.01.T01 | AT1G03920 | 75.277 | 542 | 102 | 3 | 74 | 597 | 1 | 528 | 0.0 | 817 |
| MsG0180003807.01.T01 | AT1G03920 | 75.277 | 542 | 102 | 3 | 74 | 597 | 1 | 528 | 0.0 | 817 |
Find 219 sgRNAs with CRISPR-Local
Find 863 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTGGCAAAAGAACAGG+AGG | 0.690348 | 1:-68525530 | MsG0180003807.01.T01:CDS |
| AAAAGAAACGTACCCATCCC+AGG | 0.587454 | 1:+68521979 | None:intergenic |
| AAAAGCTGGTCCATGGAGGA+AGG | 0.592704 | 1:-68523036 | MsG0180003807.01.T01:CDS |
| AAAAGGTGCTGATGAAATAA+AGG | 0.383499 | 1:-68523390 | MsG0180003807.01.T01:intron |
| AAAATCATCAACACCCATCT+TGG | 0.456102 | 1:+68527225 | None:intergenic |
| AAAGAAGTTGGCTGATGCCG+AGG | 0.601692 | 1:-68527323 | MsG0180003807.01.T01:CDS |
| AAATCAGAGATGCTTCGCAG+AGG | 0.699794 | 1:-68526328 | MsG0180003807.01.T01:CDS |
| AAATGAAGAATCTTCAGGAG+AGG | 0.618226 | 1:-68529551 | MsG0180003807.01.T01:CDS |
| AAATGGATTCTGCGAGGAGT+TGG | 0.481744 | 1:-68529746 | MsG0180003807.01.T01:CDS |
| AAATGTGAAAATTTGATGAA+AGG | 0.571299 | 1:+68516308 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGTGGAAGAAATAAGTC+CGG | + | Chr1:68520639-68520658 | None:intergenic | 30.0% | |
| AAAGACTGCAAAACAAATCT+AGG | + | Chr1:68527880-68527899 | None:intergenic | 30.0% | |
| AAATGATTAAATCTGGCACA+TGG | - | Chr1:68516502-68516521 | MsG0180003807.01.T01:CDS | 30.0% | |
| AACAAGAACAATTGCAAAAC+TGG | - | Chr1:68520147-68520166 | MsG0180003807.01.T01:CDS | 30.0% | |
| AACAAGATGTTAACATTGGA+GGG | - | Chr1:68529817-68529836 | MsG0180003807.01.T01:intron | 30.0% | |
| AACAGCGAGAAAATAAGTTA+TGG | - | Chr1:68517770-68517789 | MsG0180003807.01.T01:intron | 30.0% | |
| AACAGTAAAATAGTCACAGA+GGG | - | Chr1:68517836-68517855 | MsG0180003807.01.T01:intron | 30.0% | |
| AACAGTTGAAAATGGAGTTA+GGG | + | Chr1:68517279-68517298 | None:intergenic | 30.0% | |
| AAGACTGCAAAACAAATCTA+GGG | + | Chr1:68527879-68527898 | None:intergenic | 30.0% | |
| AAGATTCATCTGAGTATGAA+TGG | + | Chr1:68521535-68521554 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 68515485 | 68530229 | 68515485 | ID=MsG0180003807.01;Name=MsG0180003807.01 |
| Chr1 | mRNA | 68515485 | 68530229 | 68515485 | ID=MsG0180003807.01.T01;Parent=MsG0180003807.01;Name=MsG0180003807.01.T01;_AED=0.41;_eAED=0.42;_QI=20|0.85|0.8|1|0.92|0.86|15|0|1115 |
| Chr1 | exon | 68515485 | 68516920 | 68515485 | ID=MsG0180003807.01.T01:exon:51938;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68520061 | 68520171 | 68520061 | ID=MsG0180003807.01.T01:exon:51937;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68520311 | 68520367 | 68520311 | ID=MsG0180003807.01.T01:exon:51936;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68521992 | 68522076 | 68521992 | ID=MsG0180003807.01.T01:exon:51935;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68522998 | 68523084 | 68522998 | ID=MsG0180003807.01.T01:exon:51934;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68523178 | 68523291 | 68523178 | ID=MsG0180003807.01.T01:exon:51933;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68523391 | 68523522 | 68523391 | ID=MsG0180003807.01.T01:exon:51932;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68524821 | 68524905 | 68524821 | ID=MsG0180003807.01.T01:exon:51931;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68525211 | 68525290 | 68525211 | ID=MsG0180003807.01.T01:exon:51930;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68525524 | 68525740 | 68525524 | ID=MsG0180003807.01.T01:exon:51929;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68525964 | 68526205 | 68525964 | ID=MsG0180003807.01.T01:exon:51928;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68526324 | 68526407 | 68526324 | ID=MsG0180003807.01.T01:exon:51927;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68527186 | 68527360 | 68527186 | ID=MsG0180003807.01.T01:exon:51926;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68529543 | 68529804 | 68529543 | ID=MsG0180003807.01.T01:exon:51925;Parent=MsG0180003807.01.T01 |
| Chr1 | exon | 68530029 | 68530229 | 68530029 | ID=MsG0180003807.01.T01:exon:51924;Parent=MsG0180003807.01.T01 |
| Chr1 | five_prime_UTR | 68530210 | 68530229 | 68530210 | ID=MsG0180003807.01.T01:five_prime_utr;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68530029 | 68530209 | 68530029 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68529543 | 68529804 | 68529543 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68527186 | 68527360 | 68527186 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68526324 | 68526407 | 68526324 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68525964 | 68526205 | 68525964 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68525524 | 68525740 | 68525524 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68525211 | 68525290 | 68525211 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68524821 | 68524905 | 68524821 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68523391 | 68523522 | 68523391 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68523178 | 68523291 | 68523178 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68522998 | 68523084 | 68522998 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68521992 | 68522076 | 68521992 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68520311 | 68520367 | 68520311 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68520061 | 68520171 | 68520061 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Chr1 | CDS | 68515485 | 68516920 | 68515485 | ID=MsG0180003807.01.T01:cds;Parent=MsG0180003807.01.T01 |
| Gene Sequence |
>MsG0180003807.01.T01 ATGATTTCCATTAATTTCAATTTCATTTTCATTCAGCAGAAAGAAAAGACAAGAACATCGCACAGAAAATCACGCGCATCTCATCCGAAAGATACAATACATCTCTTCCTCTTCCTTTCTCATTCGCCATTCGCAATTTGGATTTTACGCTTTCCGAAATGCCCCAAGCAATTCAATTCAGAAGGAACAAGGAAGGTTGTTGATTATCGAGACAAAGAAATGGATTCTGCGAGGAGTTGGCTTTCGAAGTTGCAGCCTCGTGATAAAATGAAGGCGAAGAAGAGGGATGAGGATGGTAATAATGATGGAAATGGAGATTTGACTTCACCTGTGGATGATGGGTCGCTTTCTGATGTCACGAAGCAGAAGGTTGCGGCAGCAAAGCAGTATATCGAAAATCACTACAAGGAGCAAATGAAGAATCTTCAGGAGAGGAAAGAGCGTCGAACCATCTTGGAAAAGAAGTTGGCTGATGCCGAGGTCTCCGAAGAAGATCAAAACAACTTACTTAAATTTTTAGAGAAAAAGGAAACTGAATATATGCGCCTTCAAAGAGCCAAGATGGGTGTTGATGATTTTGAGTTATTGACTATGATTGGAAAAGGTGCATTTGGGGAGGTCAGAGTTTGCAGAGAGAAGACTACTGATCATGTATACGCAATGAAAAAACTAAAGAAATCAGAGATGCTTCGCAGAGGACAGGTTGAACATGTCAAAGCGGAAAGGAATCTCCTCGCAGAGGTTGACAGCAATTGCATTGTCAAACTTTATTGCTCTTTCCAGGATGACGAGTATCTTTATCTTATTATGGAGTATCTACCGGGTGGAGATATGATGACTCTACTTATGAGAAAGGATACCTTGACTGAAGATGAAGCCAGATTTTATGTAGGAGAAACTGTTCTGGCTATTGAATCTATACACAAGCACAATTATATACATAGGGATATCAAGCCTGATAACTTATTACTTGATAGATATGGACACCTGAGACTGTCTGACTTTGGACTTTGTAAACCATTAGATTGTAGTACGCTTGAAGAAAAGGATTTTTCTGTGGGTCAGAATGTGAATGGATCTACACAGAGTGAGGAGCGCTCAGCTCCAAAACGCACACAACAAGAACAATTGCAAAACTGGCAAAAGAACAGGAGGACGCTTGCTTATTCCACTGTTGGTACACCGGATTATATTGCGCCAGAAGTTCTTTTGAAGAAAGGGTATGGGATGGAATGTGATTGGTGGTCACTTGGTGCTATCATGTATGAAATGTTGGTGGGATATCCACCTTTTTATTCTGATGATCCAATGACAACATGTAGGAAGATAGTGAACTGGAAATCTCACTTGAAATTTCCTGATGAAGCTGGGCTATCACCTGAGGCTAAAGATCTTATCAGTAGACTTTTATGTAATGTCAACCATAGGCTGGGATCAAAAGGTGCTGATGAAATAAAGGCTCATCGGTTCTTCAAAGGTGTTGAATGGGATAAACTATATCAAATTGAAGCTGCATTTATACCTGAGGTCAATGATGAATTAGATACTCAGAATTTTGAAAAGTTTGATGAGGCTGACAGCCAAACTCGACCGTCGTCAAAAGCTGGTCCATGGAGGAAGGTAATCTTTGATGTTCCCTCTGCTGGAAATTTTCACCAAATGCTTTCATCTAAAGACTTAAATTTTGTTGGATACACATATAAGAACTTTGAAATTGTCAACGATTATCAAGTTCCTGGGATGGCTGAGCTGAAGAAGAAGCCCTCCAAATCAAAGAGACCATCCATTAAGTCGCTTTTCGATTCCGAGTCAGAGACGTCAGAGTTGTCTGAGGTTTCTGATACGTCTGCAAGTGATCGACCTCAAGGAAGCTTTTTGAATCTTTTGCCACCCAAGTTAGAGGCATCTCATAGATTTGAAGTCCTTCATGCAAATCCAAACATGATTCATCCACACATGCATGAAACATGGGACTATACAATCATTGCAAATGACTTATTATTGCCACCTCCAATCCAAACAAATCCTTATCCAAAACCACCAACAGAAAATCACTCCCTAAACTTGGGAGGACAGAATAATGAGGTGAGTGATTGGGTTGAACACATTACTAAACACTTTGTTGAAGATCAATTACCACAAAACACTTCTGATAATAATAGTTACAATAATCTTCAACAAATTCAAACAAACACCTCAGATTTATTTCAAAACCATAATAGTAATAGTGGTTATGAATGTGATCAAGGGTTGAATTTGATAACCCTTCTAATGGAATGTGCAGTGGCAATTTCAGTTGAAAATCATGGTGAGGCTCATAGAATGTTACTAGAGTTAACACAATTAGCTTCACCTTACAAAACATCATGTGCTGAACGTGTTGTTGCTTATTTTGCAAAAGCTATGATTAGTAGAGTGATGAATACATGGCTTGGAGCATGTTCTCCTTTGATTGATCATAGAACCATTCATTCTTCTCTACAAGTGTTCAACAATATTTCCCCTTTCATCAAATTTTCACATTTCACTTCAAACCAAGCAATTCTCGAAGCCGTGAACCGTTGTAATAGCATTCATATCATTGATTTGGATATCATGCAAGGGCTTCAATGGCCTGCTTTTTTTCACATACTCGCGACTCGAATGGAGGGTCGGCCTTCAGTTCGAATGACCGGGATGGGAGCATCGATGGAACTTCTTGTTGAGACGGGGAAAAATCTTTCGAATTTTGCAAGAAGGCTCGGTTTGTGTTTAGAGTTTTACCCTATAGCATGTAAATTTGGTGAAGTTGTTGATGTTTCCATGTTACAAATTAGGCCTAATGAGACACTTGCAGTTCATTGGTTGCAACATTCTTTGTATGATTCAACTGGACCTGATTGGAAAACTTTGAGACTTCTTGAAGAATTGGAACCAAAAATCATAACTTTGGTAGAACAAGATGTTAACATTGGAGGGTCATTTTTGGACCGTTTTGTTGGTTCATTGCATTACTATTCCACCCTTTTTGATTCACTTGGATCATATTTGCATAGTGATGATTCTAACAGGAACATTGTTGAACATGGTCTTCTATCTAAAGAGATCAACAATATATTAGCAATTGGTGGACCTAAGAGAAGTGGTGAAGAAAAGTTTAGGCTTTGGAGAAGTGAACTTGTTGCAAGAAATAGTTTTGAGCAGGTTCCAATGAGTGCTAATTCAATGGCTCAAGCACAGTTGATATTGAACATGTATTCACCAGCTCATGGTTATAGTCTTGCTCAAGTTGATGGCATGTTAAGGCTTGGTTGGAAAGATACTAGTTTGTATACTGCTTCTTCTTGGACTTGTAGAACTTCTAGGTAG |
| Protein sequence |
>MsG0180003807.01.T01 MISINFNFIFIQQKEKTRTSHRKSRASHPKDTIHLFLFLSHSPFAIWILRFPKCPKQFNSEGTRKVVDYRDKEMDSARSWLSKLQPRDKMKAKKRDEDGNNDGNGDLTSPVDDGSLSDVTKQKVAAAKQYIENHYKEQMKNLQERKERRTILEKKLADAEVSEEDQNNLLKFLEKKETEYMRLQRAKMGVDDFELLTMIGKGAFGEVRVCREKTTDHVYAMKKLKKSEMLRRGQVEHVKAERNLLAEVDSNCIVKLYCSFQDDEYLYLIMEYLPGGDMMTLLMRKDTLTEDEARFYVGETVLAIESIHKHNYIHRDIKPDNLLLDRYGHLRLSDFGLCKPLDCSTLEEKDFSVGQNVNGSTQSEERSAPKRTQQEQLQNWQKNRRTLAYSTVGTPDYIAPEVLLKKGYGMECDWWSLGAIMYEMLVGYPPFYSDDPMTTCRKIVNWKSHLKFPDEAGLSPEAKDLISRLLCNVNHRLGSKGADEIKAHRFFKGVEWDKLYQIEAAFIPEVNDELDTQNFEKFDEADSQTRPSSKAGPWRKVIFDVPSAGNFHQMLSSKDLNFVGYTYKNFEIVNDYQVPGMAELKKKPSKSKRPSIKSLFDSESETSELSEVSDTSASDRPQGSFLNLLPPKLEASHRFEVLHANPNMIHPHMHETWDYTIIANDLLLPPPIQTNPYPKPPTENHSLNLGGQNNEVSDWVEHITKHFVEDQLPQNTSDNNSYNNLQQIQTNTSDLFQNHNSNSGYECDQGLNLITLLMECAVAISVENHGEAHRMLLELTQLASPYKTSCAERVVAYFAKAMISRVMNTWLGACSPLIDHRTIHSSLQVFNNISPFIKFSHFTSNQAILEAVNRCNSIHIIDLDIMQGLQWPAFFHILATRMEGRPSVRMTGMGASMELLVETGKNLSNFARRLGLCLEFYPIACKFGEVVDVSMLQIRPNETLAVHWLQHSLYDSTGPDWKTLRLLEELEPKIITLVEQDVNIGGSFLDRFVGSLHYYSTLFDSLGSYLHSDDSNRNIVEHGLLSKEINNILAIGGPKRSGEEKFRLWRSELVARNSFEQVPMSANSMAQAQLILNMYSPAHGYSLAQVDGMLRLGWKDTSLYTASSWTCRTSR* |
