AlfalfaGEDB | MsG0080047958.01

M. sativa cultivar ZhongmuNo.1 / MsG0080047958.01

KEGG: K13116
GO: GO:0003676 GO:0005524
PFAM: PF00270

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080047958.01.T01	QHO44078.1	98.058	103	2	0	1	103	295	397	2.43E-64	209

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080047958.01.T01	Q5Z6G5	93.939	99	6	0	1	99	325	423	9.88E-60	195

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080047958.01.T01	A0A2K3KI53	98.990	99	1	0	1	99	113	211	5.04e-63	201

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047958.01	MsG0080048672.01	0.813670	2.275253e-51	1.495246e-48
MsG0080047958.01	MsG0180000091.01	0.815780	7.755863e-52	5.398129e-49
MsG0080047958.01	MsG0180000703.01	0.816227	6.163922e-52	4.342616e-49
MsG0080047958.01	MsG0180000836.01	0.810707	1.008026e-50	6.120369e-48
MsG0080047958.01	MsG0180003725.01	0.806532	7.860225e-50	4.276516e-47
MsG0080047958.01	MsG0180003807.01	0.816861	4.445507e-52	3.186701e-49
MsG0080047958.01	MsG0180004331.01	0.805905	1.065113e-49	5.703085e-47
MsG0080047958.01	MsG0180005445.01	0.813670	2.276139e-51	1.495800e-48
MsG0080047958.01	MsG0180005730.01	0.810998	8.719535e-51	5.335924e-48
MsG0080047958.01	MsG0280006461.01	0.802251	6.129395e-49	2.988267e-46
MsG0080047958.01	MsG0280006601.01	0.800701	1.273955e-48	5.971333e-46
MsG0080047958.01	MsG0280008363.01	0.819072	1.406942e-52	1.071735e-49
MsG0080047958.01	MsG0280008738.01	0.804297	2.311544e-49	1.187530e-46
MsG0080047958.01	MsG0280009350.01	0.804789	1.824996e-49	9.495657e-47
MsG0080047958.01	MsG0280010109.01	0.810466	1.136727e-50	6.857173e-48
MsG0080047958.01	MsG0280010517.01	0.820413	6.951486e-53	5.496791e-50
MsG0080047958.01	MsG0280010599.01	0.826948	2.050124e-54	1.951092e-51
MsG0080047958.01	MsG0280010816.01	0.802760	4.813678e-49	2.377501e-46
MsG0080047958.01	MsG0280011444.01	0.801923	7.161039e-49	3.462074e-46
MsG0080047958.01	MsG0380013176.01	0.818939	1.508834e-52	1.145306e-49
MsG0080047958.01	MsG0380015300.01	0.801740	7.808873e-49	3.757593e-46
MsG0080047958.01	MsG0380016387.01	0.802161	6.397295e-49	3.111713e-46
MsG0080047958.01	MsG0380016694.01	0.805504	1.292932e-49	6.851743e-47
MsG0080047958.01	MsG0380016923.01	0.825114	5.591792e-54	5.050189e-51
MsG0080047958.01	MsG0380017401.01	0.818294	2.112719e-52	1.575155e-49
MsG0080047958.01	MsG0480018479.01	0.819086	1.397165e-52	1.064693e-49
MsG0080047958.01	MsG0480019035.01	0.813623	2.330571e-51	1.529661e-48
MsG0080047958.01	MsG0480021918.01	0.809674	1.684042e-50	9.950044e-48
MsG0080047958.01	MsG0580025661.01	0.804726	1.880817e-49	9.770670e-47
MsG0080047958.01	MsG0580029376.01	0.802450	5.578972e-49	2.733669e-46
MsG0080047958.01	MsG0580029647.01	0.804685	1.918479e-49	9.955050e-47
MsG0080047958.01	MsG0580029651.01	0.810520	1.106544e-50	6.684588e-48
MsG0080047958.01	MsG0680030413.01	0.808753	2.652202e-50	1.528978e-47
MsG0080047958.01	MsG0680032112.01	0.815004	1.154042e-51	7.863021e-49
MsG0080047958.01	MsG0680034235.01	0.815429	9.287315e-52	6.401621e-49
MsG0080047958.01	MsG0680034913.01	0.801335	9.451261e-49	4.501893e-46
MsG0080047958.01	MsG0780036430.01	0.811432	7.020307e-51	4.345052e-48
MsG0080047958.01	MsG0780036773.01	0.811515	6.734713e-51	4.177492e-48
MsG0080047958.01	MsG0780039170.01	0.804452	2.145534e-49	1.106625e-46
MsG0080047958.01	MsG0780039443.01	0.822115	2.814607e-53	2.334147e-50
MsG0080047958.01	MsG0780040254.01	0.801108	1.052107e-48	4.983040e-46
MsG0080047958.01	MsG0780040888.01	0.811206	7.861047e-51	4.836401e-48
MsG0080047958.01	MsG0780041387.01	0.807297	5.415984e-50	3.005588e-47
MsG0080047958.01	MsG0780041482.01	0.815314	9.850018e-52	6.767972e-49
MsG0080047958.01	MsG0880043480.01	0.806770	6.999675e-50	3.831644e-47
MsG0080047958.01	MsG0880043521.01	0.815002	1.155394e-51	7.871712e-49
MsG0080047958.01	MsG0880044948.01	0.802477	5.506871e-49	2.700200e-46
MsG0080047958.01	MsG0880046901.01	0.806163	9.400897e-50	5.067054e-47
MsG0080047958.01	MsG0880047696.01	0.808316	3.287822e-50	1.873962e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080047958.01.T01	MTR_1g026830	100.000	99	0	0	1	99	298	396	6.19e-65	206
MsG0080047958.01.T01	MTR_1g095810	98.990	99	1	0	1	99	296	394	2.27e-63	201
MsG0080047958.01.T01	MTR_7g109720	47.826	92	47	1	8	98	381	472	2.46e-21	87.4
MsG0080047958.01.T01	MTR_3g467550	41.053	95	56	0	2	96	230	324	2.61e-18	79.0
MsG0080047958.01.T01	MTR_3g083690	41.584	101	55	1	1	97	293	393	2.67e-18	79.0
MsG0080047958.01.T01	MTR_3g467550	41.053	95	56	0	2	96	261	355	3.34e-18	78.6
MsG0080047958.01.T01	MTR_3g467550	41.053	95	56	0	2	96	261	355	4.87e-18	77.8
MsG0080047958.01.T01	MTR_1g054630	42.574	101	54	1	1	97	301	401	2.29e-17	76.3
MsG0080047958.01.T01	MTR_6g005550	38.776	98	55	2	1	93	306	403	3.26e-17	75.9
MsG0080047958.01.T01	MTR_4g114120	38.235	102	59	1	1	98	293	394	5.43e-17	75.1
MsG0080047958.01.T01	MTR_2g007000	43.750	80	45	0	2	81	650	729	4.85e-16	72.4
MsG0080047958.01.T01	MTR_2g032630	43.902	82	44	1	9	88	645	726	5.68e-16	72.0
MsG0080047958.01.T01	MTR_4g105100	39.796	98	58	1	2	98	345	442	4.17e-15	69.7
MsG0080047958.01.T01	MTR_4g105100	39.796	98	58	1	2	98	345	442	4.69e-15	69.7
MsG0080047958.01.T01	MTR_2g100230	38.144	97	59	1	2	97	239	335	2.13e-14	67.8
MsG0080047958.01.T01	MTR_4g134170	33.607	122	60	2	3	103	434	555	8.19e-14	65.9
MsG0080047958.01.T01	MTR_6g005320	33.607	122	60	2	3	103	396	517	1.03e-13	65.9
MsG0080047958.01.T01	MTR_5g090120	39.394	66	40	0	1	66	222	287	1.74e-13	65.1
MsG0080047958.01.T01	MTR_3g449250	37.879	66	41	0	1	66	231	296	1.78e-12	62.0
MsG0080047958.01.T01	MTR_3g449250	37.879	66	41	0	1	66	231	296	2.07e-12	62.0
MsG0080047958.01.T01	MTR_5g027840	36.000	100	62	2	3	100	249	348	1.82e-11	59.3
MsG0080047958.01.T01	MTR_7g076820	40.625	64	38	0	3	66	289	352	6.69e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080047958.01.T01	AT5G51280	92.929	99	7	0	1	99	298	396	5.19e-59	190
MsG0080047958.01.T01	AT4G33370	88.889	99	11	0	1	99	249	347	2.03e-55	180
MsG0080047958.01.T01	AT2G47330	50.000	90	44	1	8	96	378	467	3.31e-21	87.0
MsG0080047958.01.T01	AT1G20920	40.000	95	57	0	2	96	338	432	1.60e-18	79.3
MsG0080047958.01.T01	AT1G20920	40.000	95	57	0	2	96	676	770	2.83e-18	79.0
MsG0080047958.01.T01	AT1G20920	40.000	95	57	0	2	96	676	770	2.83e-18	79.0
MsG0080047958.01.T01	AT1G20920	40.000	95	57	0	2	96	676	770	2.83e-18	79.0
MsG0080047958.01.T01	AT1G20920	40.000	95	57	0	2	96	676	770	2.83e-18	79.0
MsG0080047958.01.T01	AT1G20920	40.000	95	57	0	2	96	676	770	2.83e-18	79.0
MsG0080047958.01.T01	AT1G20920	40.000	95	57	0	2	96	676	770	2.83e-18	79.0
MsG0080047958.01.T01	AT3G09620	41.053	95	56	0	2	96	543	637	5.65e-18	77.8
MsG0080047958.01.T01	AT3G09620	41.053	95	56	0	2	96	544	638	5.65e-18	77.8
MsG0080047958.01.T01	AT2G42520	42.105	95	51	1	7	97	313	407	1.33e-17	77.0
MsG0080047958.01.T01	AT2G42520	42.105	95	51	1	7	97	313	407	1.33e-17	77.0
MsG0080047958.01.T01	AT2G42520	42.105	95	51	1	7	97	313	407	1.33e-17	77.0
MsG0080047958.01.T01	AT3G58570	39.604	101	57	1	1	97	294	394	3.80e-17	75.5
MsG0080047958.01.T01	AT3G06480	45.122	82	43	1	9	88	585	666	7.77e-17	74.7
MsG0080047958.01.T01	AT3G06480	45.122	82	43	1	9	88	585	666	7.92e-17	74.7
MsG0080047958.01.T01	AT3G58510	40.000	95	53	1	7	97	305	399	2.47e-16	73.2
MsG0080047958.01.T01	AT3G58510	40.000	95	53	1	7	97	305	399	2.47e-16	73.2
MsG0080047958.01.T01	AT3G58510	40.000	95	53	1	7	97	305	399	2.47e-16	73.2
MsG0080047958.01.T01	AT5G14610	39.759	83	48	1	1	81	311	393	4.10e-15	69.7
MsG0080047958.01.T01	AT5G14610	39.759	83	48	1	1	81	172	254	4.12e-15	69.7
MsG0080047958.01.T01	AT5G14610	39.759	83	48	1	1	81	172	254	4.12e-15	69.7
MsG0080047958.01.T01	AT5G14610	39.759	83	48	1	1	81	303	385	4.21e-15	69.7
MsG0080047958.01.T01	AT5G14610	39.759	83	48	1	1	81	303	385	4.21e-15	69.7
MsG0080047958.01.T01	AT5G14610	39.759	83	48	1	1	81	311	393	4.22e-15	69.7
MsG0080047958.01.T01	AT5G14610	39.759	83	48	1	1	81	371	453	4.25e-15	69.7
MsG0080047958.01.T01	AT3G01540	40.000	85	49	1	1	83	300	384	6.25e-15	69.3
MsG0080047958.01.T01	AT3G01540	40.000	85	49	1	1	83	300	384	6.25e-15	69.3
MsG0080047958.01.T01	AT3G01540	40.000	85	49	1	1	83	300	384	6.25e-15	69.3
MsG0080047958.01.T01	AT3G01540	40.000	85	49	1	1	83	300	384	6.25e-15	69.3
MsG0080047958.01.T01	AT5G63120	39.796	98	58	1	2	98	309	406	6.32e-15	69.3
MsG0080047958.01.T01	AT5G63120	39.796	98	58	1	2	98	309	406	6.48e-15	69.3
MsG0080047958.01.T01	AT5G63120	39.796	98	58	1	2	98	309	406	6.76e-15	68.9
MsG0080047958.01.T01	AT5G63120	39.796	98	58	1	2	98	309	406	6.76e-15	68.9
MsG0080047958.01.T01	AT5G63120	39.796	98	58	1	2	98	309	406	6.76e-15	68.9
MsG0080047958.01.T01	AT1G55150	36.082	97	61	1	2	97	243	339	1.60e-14	68.2
MsG0080047958.01.T01	AT1G55150	36.082	97	61	1	2	97	268	364	1.90e-14	67.8
MsG0080047958.01.T01	AT2G33730	33.607	122	60	2	3	103	461	582	4.10e-14	67.0
MsG0080047958.01.T01	AT1G28180	46.154	65	35	0	3	67	121	185	1.30e-13	65.5
MsG0080047958.01.T01	AT5G65900	33.333	96	62	1	5	98	301	396	3.80e-13	64.3
MsG0080047958.01.T01	AT1G31970	35.294	102	64	2	1	100	261	362	1.19e-12	62.8
MsG0080047958.01.T01	AT5G26742	37.879	66	41	0	1	66	155	220	5.93e-12	60.8
MsG0080047958.01.T01	AT5G26742	37.879	66	41	0	1	66	248	313	6.17e-12	60.8
MsG0080047958.01.T01	AT5G26742	37.879	66	41	0	1	66	248	313	6.17e-12	60.8
MsG0080047958.01.T01	AT3G18600	34.783	92	58	1	9	98	240	331	2.35e-11	58.9
MsG0080047958.01.T01	AT5G60990	30.189	106	70	1	2	103	161	266	2.39e-11	58.9
MsG0080047958.01.T01	AT3G22310	33.333	66	44	0	1	66	258	323	2.51e-11	58.9
MsG0080047958.01.T01	AT3G22310	33.333	66	44	0	1	66	258	323	2.51e-11	58.9
MsG0080047958.01.T01	AT1G77030	33.333	93	62	0	1	93	169	261	5.14e-11	58.2

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTAGCAAAATTCTGAATTT+TGG	0.133852	contig152end:-361505	None:intergenic
CGTCCCACATTGACAATAAT+AGG	0.278247	contig152end:-361543	None:intergenic
GGCGAATCTTGATGTTATTC+AGG	0.315986	contig152end:+361575	MsG0080047957.01.T01:intron
GGCTGATCGATTGGTAGATC+CGG	0.411884	contig152end:+361401	MsG0080047957.01.T01:intron
ATTGTCAATGTGGGACGTGC+AGG	0.426137	contig152end:+361549	MsG0080047957.01.T01:intron
TTCTCACAAAATATCAAAAC+AGG	0.435087	contig152end:-361665	None:intergenic
CAAAATATCAAAACAGGAGG+TGG	0.445015	contig152end:-361659	None:intergenic
CTTATATCATCTTCAAATCC+CGG	0.494897	contig152end:-361420	None:intergenic
AAATGAATCTTGATAACTGC+AGG	0.497521	contig152end:+361361	MsG0080047957.01.T01:intron
GTTAGATGAGGCTGATCGAT+TGG	0.534300	contig152end:+361392	MsG0080047957.01.T01:intron
TTGTCAATGTGGGACGTGCA+GGG	0.535671	contig152end:+361550	MsG0080047957.01.T01:intron
CAATGTGGGACGTGCAGGGG+CGG	0.535678	contig152end:+361554	MsG0080047957.01.T01:intron
GCTGATCGATTGGTAGATCC+GGG	0.548897	contig152end:+361402	MsG0080047957.01.T01:intron
AAAACCTATTATTGTCAATG+TGG	0.574587	contig152end:+361539	MsG0080047957.01.T01:intron
AAAATATCAAAACAGGAGGT+GGG	0.579260	contig152end:-361658	None:intergenic
TTGATCACTTCAAAGCTCAA+AGG	0.591719	contig152end:+361451	MsG0080047957.01.T01:intron
TGTCAATGTGGGACGTGCAG+GGG	0.592163	contig152end:+361551	MsG0080047957.01.T01:intron
AAACCTATTATTGTCAATGT+GGG	0.592275	contig152end:+361540	MsG0080047957.01.T01:intron
CAGGTATTTAACGTTAGATG+AGG	0.599810	contig152end:+361380	MsG0080047957.01.T01:intron
AGTAGAGTATGTCAAACAAG+AGG	0.613269	contig152end:+361599	MsG0080047957.01.T01:intron
TCACAAAATATCAAAACAGG+AGG	0.646136	contig152end:-361662	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAACCTATTATTGTCAATG+TGG	+	contig152end:361539-361558	MsG0080047957.01.T01:intron	25.0%
!	AAACCTATTATTGTCAATGT+GGG	+	contig152end:361540-361559	MsG0080047957.01.T01:intron	25.0%
!!	TCTAGCAAAATTCTGAATTT+TGG	-	contig152end:361508-361527	None:intergenic	25.0%
	CTTATATCATCTTCAAATCC+CGG	-	contig152end:361423-361442	None:intergenic	30.0%
!!	GCAAAATTCTGAATTTTGGT+TGG	-	contig152end:361504-361523	None:intergenic	30.0%
	AGTAGAGTATGTCAAACAAG+AGG	+	contig152end:361599-361618	MsG0080047957.01.T01:intron	35.0%
	CAGGTATTTAACGTTAGATG+AGG	+	contig152end:361380-361399	MsG0080047957.01.T01:intron	35.0%
	TTGATCACTTCAAAGCTCAA+AGG	+	contig152end:361451-361470	MsG0080047957.01.T01:intron	35.0%
!!!	GAATTTTGCTAGAAGTGCTT+TGG	+	contig152end:361515-361534	MsG0080047957.01.T01:intron	35.0%
	CGTCCCACATTGACAATAAT+AGG	-	contig152end:361546-361565	None:intergenic	40.0%
	GGCGAATCTTGATGTTATTC+AGG	+	contig152end:361575-361594	MsG0080047957.01.T01:intron	40.0%
	GTTAGATGAGGCTGATCGAT+TGG	+	contig152end:361392-361411	MsG0080047957.01.T01:intron	45.0%
!	TTTTCTGTAGGCACTCAAGG+AGG	-	contig152end:361638-361657	None:intergenic	45.0%
	ATTGTCAATGTGGGACGTGC+AGG	+	contig152end:361549-361568	MsG0080047957.01.T01:intron	50.0%
	GCTGATCGATTGGTAGATCC+GGG	+	contig152end:361402-361421	MsG0080047957.01.T01:intron	50.0%
	GGCTGATCGATTGGTAGATC+CGG	+	contig152end:361401-361420	MsG0080047957.01.T01:intron	50.0%
	TTGTCAATGTGGGACGTGCA+GGG	+	contig152end:361550-361569	MsG0080047957.01.T01:intron	50.0%
!	GGGTTTTCTGTAGGCACTCA+AGG	-	contig152end:361641-361660	None:intergenic	50.0%
!!	ACAGGAGGTGGGTTTTCTGT+AGG	-	contig152end:361650-361669	None:intergenic	50.0%
	TGTCAATGTGGGACGTGCAG+GGG	+	contig152end:361551-361570	MsG0080047957.01.T01:intron	55.0%
	CAATGTGGGACGTGCAGGGG+CGG	+	contig152end:361554-361573	MsG0080047957.01.T01:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
contig152end	gene	361363	361674	361363	ID=MsG0080047958.01;Name=MsG0080047958.01
contig152end	mRNA	361363	361674	361363	ID=MsG0080047958.01.T01;Parent=MsG0080047958.01;Name=MsG0080047958.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|103
contig152end	exon	361363	361674	361363	ID=MsG0080047958.01.T01:exon:13787;Parent=MsG0080047958.01.T01
contig152end	CDS	361363	361674	361363	ID=MsG0080047958.01.T01:cds;Parent=MsG0080047958.01.T01

Gene Sequence	>MsG0080047958.01.T01 ATGAATCTTGATAACTGCAGGTATTTAACGTTAGATGAGGCTGATCGATTGGTAGATCCGGGATTTGAAGATGATATAAGAGAAGTTTTTGATCACTTCAAAGCTCAAAGGCAGACTCTCTTGTTTTCTGCTACTATGCCAACCAAAATTCAGAATTTTGCTAGAAGTGCTTTGGTAAAACCTATTATTGTCAATGTGGGACGTGCAGGGGCGGCGAATCTTGATGTTATTCAGGAAGTAGAGTATGTCAAACAAGAGGCAAAAATTGTTTACCTCCTTGAGTGCCTACAGAAAACCCACCTCCTGTTTTGA
Protein sequence	>MsG0080047958.01.T01 MNLDNCRYLTLDEADRLVDPGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPIIVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTHLLF*