AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004685.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004685.01.T01 MTR_1g084740 85.837 233 4 1 1 233 1 204 9.05e-146 406
MsG0180004685.01.T01 MTR_8g065050 56.780 236 68 4 1 235 1 203 5.01e-89 262
MsG0180004685.01.T01 MTR_1g084740 98.901 91 1 0 1 91 1 91 3.52e-59 185
MsG0180004685.01.T01 MTR_1g084740 92.188 64 5 0 172 235 92 155 1.87e-38 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004685.01.T01 AT1G56700 60.426 235 61 3 1 235 1 203 9.48e-101 292
MsG0180004685.01.T01 AT1G56700 60.426 235 61 3 1 235 1 203 9.48e-101 292
MsG0180004685.01.T01 AT1G56700 60.426 235 61 3 1 235 1 203 9.48e-101 292
MsG0180004685.01.T01 AT1G23440 55.603 232 70 3 1 232 1 199 1.91e-90 266
MsG0180004685.01.T01 AT1G23440 55.603 232 70 3 1 232 1 199 1.91e-90 266
MsG0180004685.01.T01 AT1G56700 58.824 204 54 2 32 235 1 174 5.28e-84 249
MsG0180004685.01.T01 AT1G23440 53.191 94 40 2 1 94 1 90 2.11e-29 106

Find 83 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTTCCAATAAAATTATC+TGG 0.221027 1:+81212182 MsG0180004685.01.T01:CDS
ATGTTTGTTCAGCTGCATTT+TGG 0.221330 1:+81212568 MsG0180004685.01.T01:intron
CAAGAATACGGGAGGTAAAT+TGG 0.241772 1:+81212808 MsG0180004685.01.T01:CDS
ATTGTCTTCATTCCATTTCC+TGG 0.292689 1:+81210977 None:intergenic
ATTTGCTGCTTCCTTATTAG+AGG 0.294466 1:+81213444 MsG0180004685.01.T01:CDS
ACCTCCCGTATTCTTGAAAT+CGG 0.310925 1:-81212801 None:intergenic
CTTCCGTTGCCCTGATGAAA+TGG 0.315906 1:+81212648 MsG0180004685.01.T01:CDS
AGGAAGCAGCAAATTGCATT+TGG 0.316795 1:-81213435 None:intergenic
ATTAGCAAGTACCTCTAATA+AGG 0.317751 1:-81213455 None:intergenic
GTAACAACAGTTTATATAAC+AGG 0.332893 1:+81211959 MsG0180004685.01.T01:CDS
TCCATTTCCTGGTAAGGAAT+AGG 0.336492 1:+81210988 MsG0180004685.01.T01:five_prime_UTR
GGCTTCCATCCCATTTCATC+AGG 0.344197 1:-81212658 None:intergenic
CTGAGGCAAGCTGACATCTT+AGG 0.349819 1:+81213648 MsG0180004685.01.T01:three_prime_UTR
TCCTATTCCTTACCAGGAAA+TGG 0.372630 1:-81210989 None:intergenic
AATAAGATATTGTTATGAAA+TGG 0.383641 1:+81213585 MsG0180004685.01.T01:three_prime_UTR
CCCAGCTCATGTAATTGTTC+TGG 0.386886 1:+81213488 MsG0180004685.01.T01:three_prime_UTR
AATTTATAACATTTCATTCA+AGG 0.392637 1:-81213545 None:intergenic
TGCCAAAGGGTTTAGCTATC+GGG 0.396835 1:+81212065 MsG0180004685.01.T01:CDS
TTCAGATTCCTTGGCAATAA+CGG 0.399064 1:-81212155 None:intergenic
CTATCATTCTCTTCGGTTCG+CGG 0.401359 1:+81213349 MsG0180004685.01.T01:CDS
ACCTTTCGTTGCCAAGGTCT+TGG 0.402676 1:-81213273 None:intergenic
CTTCATTCCATTTCCTGGTA+AGG 0.413462 1:+81210982 None:intergenic
GGAAGCAGCAAATTGCATTT+GGG 0.414368 1:-81213434 None:intergenic
TCTCTCGAGTTCGTAGTGTT+TGG 0.424651 1:+81212865 MsG0180004685.01.T01:CDS
ATGTTTACTATCATTCTCTT+CGG 0.426824 1:+81213342 MsG0180004685.01.T01:CDS
ACCAGAACAATTACATGAGC+TGG 0.432033 1:-81213489 None:intergenic
CGGACCCCATCTGCGGATGA+TGG 0.434904 1:-81211919 None:intergenic
ACTTGAAGATTCAGATTCCT+TGG 0.436551 1:-81212164 None:intergenic
TTGCCAAAGGGTTTAGCTAT+CGG 0.445632 1:+81212064 MsG0180004685.01.T01:CDS
TTATTCACAATTGTCTCCGT+TGG 0.459045 1:-81212013 None:intergenic
GTCTTGGTCATCTGCTCCAC+AGG 0.464244 1:-81213257 None:intergenic
ATGGCCCGATTTCAAGAATA+CGG 0.466039 1:+81212796 MsG0180004685.01.T01:CDS
TGTTGTCATGCTTCTGAGAA+TGG 0.469604 1:+81213511 MsG0180004685.01.T01:three_prime_UTR
GTTCCCATTGTTCCTTCAGA+TGG 0.480004 1:+81212777 MsG0180004685.01.T01:CDS
GACAATTGTGAATAATTTGA+CGG 0.484102 1:+81212021 MsG0180004685.01.T01:CDS
TACAGCAGCTGAAGGACCTT+CGG 0.491990 1:-81211939 None:intergenic
CCTCCCGTATTCTTGAAATC+GGG 0.492780 1:-81212800 None:intergenic
CGGGAGGTAAATTGGATCGC+TGG 0.500551 1:+81212816 MsG0180004685.01.T01:CDS
TGTAGTATTCTTGATACTGC+TGG 0.503788 1:+81212091 MsG0180004685.01.T01:CDS
CTCCCGATAGCTAAACCCTT+TGG 0.506502 1:-81212067 None:intergenic
TAACGGCAGATTGCAATGTC+TGG 0.506661 1:-81212138 None:intergenic
ATCATAACCTTTCGTTGCCA+AGG 0.509665 1:-81213279 None:intergenic
AATCAATCCTATTCCTTACC+AGG 0.513996 1:-81210995 None:intergenic
ACAGGTTTCAAGAAATTCCA+TGG 0.515589 1:+81211977 MsG0180004685.01.T01:CDS
TTCCGTTGCCCTGATGAAAT+GGG 0.529742 1:+81212649 MsG0180004685.01.T01:CDS
AAGTTCCAATAAAATTATCT+GGG 0.531862 1:+81212183 MsG0180004685.01.T01:CDS
CGGGCCATCTGAAGGAACAA+TGG 0.539127 1:-81212781 None:intergenic
GGGCCATCTGAAGGAACAAT+GGG 0.541004 1:-81212780 None:intergenic
TGGTCATCTGCTCCACAGGA+AGG 0.549415 1:-81213253 None:intergenic
TCATTTATTGTGAAGAAGAG+TGG 0.550686 1:-81213407 None:intergenic
ATTAGACCATCATCCGCAGA+TGG 0.550739 1:+81211913 MsG0180004685.01.T01:exon
CTTGAAATCGGGCCATCTGA+AGG 0.551552 1:-81212789 None:intergenic
GTACAAATATGAAAATGTTG+AGG 0.558685 1:+81212887 MsG0180004685.01.T01:CDS
TTGGATTCTCTGAAACTCCA+TGG 0.559574 1:-81211994 None:intergenic
ATGTCTGGTACAGCGGAACA+AGG 0.561027 1:-81212123 None:intergenic
ATCCCATTTCATCAGGGCAA+CGG 0.562088 1:-81212651 None:intergenic
GCAATCTGCCGTTATTGCCA+AGG 0.564914 1:+81212147 MsG0180004685.01.T01:CDS
GATTGCAATGTCTGGTACAG+CGG 0.575127 1:-81212130 None:intergenic
CATCCGCAGATGGGGTCCGA+AGG 0.582064 1:+81211923 MsG0180004685.01.T01:exon
ACCAAGACCTTGGCAACGAA+AGG 0.585242 1:+81213272 MsG0180004685.01.T01:CDS
ATTCTTGATACTGCTGGACA+AGG 0.586542 1:+81212097 MsG0180004685.01.T01:CDS
TTAGACCATCATCCGCAGAT+GGG 0.590599 1:+81211914 MsG0180004685.01.T01:exon
GGAGCAGATGACCAAGACCT+TGG 0.592442 1:+81213262 MsG0180004685.01.T01:CDS
CTTCGGTTCGCGGAGCAGAA+CGG 0.596040 1:+81213359 MsG0180004685.01.T01:CDS
CCAGAACAATTACATGAGCT+GGG 0.598749 1:-81213488 None:intergenic
TGGCCCGATTTCAAGAATAC+GGG 0.601050 1:+81212797 MsG0180004685.01.T01:CDS
GGGTTAACAGTGGTGCAACA+AGG 0.614278 1:+81212590 MsG0180004685.01.T01:CDS
GGTCATCTGCTCCACAGGAA+GGG 0.614706 1:-81213252 None:intergenic
GGACCTTCGGACCCCATCTG+CGG 0.617904 1:-81211926 None:intergenic
TGTAGCCGATAAGTATGTCT+CGG 0.621628 1:+81213758 MsG0180004685.01.T01:three_prime_UTR
TAGACCATCATCCGCAGATG+GGG 0.622906 1:+81211915 MsG0180004685.01.T01:exon
TGAAATGGGATGGAAGCCAC+AGG 0.624135 1:+81212663 MsG0180004685.01.T01:CDS
ACTGTTGTTACAGCAGCTGA+AGG 0.629270 1:-81211947 None:intergenic
CATTTCATCAGGGCAACGGA+AGG 0.630663 1:-81212647 None:intergenic
TATGCAGACTACCCTTCCTG+TGG 0.633375 1:+81213241 MsG0180004685.01.T01:intron
GCTTCCATCCCATTTCATCA+GGG 0.640372 1:-81212657 None:intergenic
GTTGCCCTGATGAAATGGGA+TGG 0.648441 1:+81212653 MsG0180004685.01.T01:CDS
TGTCTGGTACAGCGGAACAA+GGG 0.649049 1:-81212122 None:intergenic
CCCGATTTCAAGAATACGGG+AGG 0.650919 1:+81212800 MsG0180004685.01.T01:CDS
TGACATCTGATGATGCAGGA+AGG 0.659977 1:+81213306 MsG0180004685.01.T01:CDS
GTAATGACATCTGATGATGC+AGG 0.664436 1:+81213302 MsG0180004685.01.T01:CDS
AGAACGTCAAGCTGTCAATG+AGG 0.679006 1:+81212621 MsG0180004685.01.T01:CDS
TGGAGTTTCAGAGAATCCAA+CGG 0.696242 1:+81211997 MsG0180004685.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATATGATAATATAATTA+TGG + Chr1:81212390-81212409 MsG0180004685.01.T01:intron 10.0%
!! AATAAGATATTGTTATGAAA+TGG + Chr1:81213585-81213604 MsG0180004685.01.T01:three_prime_UTR 15.0%
!! AATTTATAACATTTCATTCA+AGG - Chr1:81213548-81213567 None:intergenic 15.0%
!! TATATTATCATATCTAAACA+AGG - Chr1:81212386-81212405 None:intergenic 15.0%
!! TTATGAAATGGAAATAAAAA+TGG + Chr1:81213597-81213616 MsG0180004685.01.T01:three_prime_UTR 15.0%
!! AAGTTCCAATAAAATTATCT+GGG + Chr1:81212183-81212202 MsG0180004685.01.T01:CDS 20.0%
!! ATTCAACATGAAAACATAAT+TGG - Chr1:81211317-81211336 None:intergenic 20.0%
!! TACATAAATAAAACTTCAGA+AGG - Chr1:81212282-81212301 None:intergenic 20.0%
!! TTCAACATGAAAACATAATT+GGG - Chr1:81211316-81211335 None:intergenic 20.0%
!!! ACATAATCTTGCAAATTTTA+AGG - Chr1:81211285-81211304 None:intergenic 20.0%
!!! AGTAATTTACAGTGTTTTTT+CGG + Chr1:81213798-81213817 MsG0180004685.01.T01:three_prime_UTR 20.0%
!!! GTATTTTTAAGTGATGATTT+TGG + Chr1:81211751-81211770 MsG0180004685.01.T01:intron 20.0%
!!! TTTCTGCAATAAAAGTTTTA+TGG - Chr1:81212757-81212776 None:intergenic 20.0%
! ATCATATCTAAACAAGGATA+CGG - Chr1:81212380-81212399 None:intergenic 25.0%
! ATGTTTACTATCATTCTCTT+CGG + Chr1:81213342-81213361 MsG0180004685.01.T01:CDS 25.0%
! CAAGTTCCAATAAAATTATC+TGG + Chr1:81212182-81212201 MsG0180004685.01.T01:CDS 25.0%
! CTAAATTTCAAGACTTTACA+AGG - Chr1:81211445-81211464 None:intergenic 25.0%
! GACAATTGTGAATAATTTGA+CGG + Chr1:81212021-81212040 MsG0180004685.01.T01:CDS 25.0%
! GTAACAACAGTTTATATAAC+AGG + Chr1:81211959-81211978 MsG0180004685.01.T01:CDS 25.0%
! GTACAAATATGAAAATGTTG+AGG + Chr1:81212887-81212906 MsG0180004685.01.T01:CDS 25.0%
! TTAGCATAGAACTGAAATAA+TGG + Chr1:81212468-81212487 MsG0180004685.01.T01:intron 25.0%
! TTATCATGAATCTTATGTTC+TGG + Chr1:81212543-81212562 MsG0180004685.01.T01:intron 25.0%
! TTGTGAAATGATACTTAGAA+TGG - Chr1:81212969-81212988 None:intergenic 25.0%
!! AATGTTTCTGTATATGATGA+TGG + Chr1:81211784-81211803 MsG0180004685.01.T01:intron 25.0%
!! CTGTTCTTTAAATATTTCTG+AGG - Chr1:81212942-81212961 None:intergenic 25.0%
!! TTATGTTCTTTTGTATTTCG+AGG + Chr1:81211114-81211133 MsG0180004685.01.T01:five_prime_UTR 25.0%
!!! ATGTTTTTTTTCTTCTTCTG+AGG - Chr1:81211079-81211098 None:intergenic 25.0%
!!! GTTGTTAAGATTTTAAGTTG+TGG + Chr1:81211484-81211503 MsG0180004685.01.T01:intron 25.0%
!!! TAAGATTTTAAGTTGTGGTT+TGG + Chr1:81211489-81211508 MsG0180004685.01.T01:intron 25.0%
!!! TTTTACTTGTCATTTAATGC+AGG + Chr1:81211851-81211870 MsG0180004685.01.T01:intron 25.0%
AAAACATGGAACAGTCATTA+AGG - Chr1:81213174-81213193 None:intergenic 30.0%
AACATGAAAACATAATTGGG+TGG - Chr1:81211313-81211332 None:intergenic 30.0%
ACGGAATATGTGAAAAAGAA+GGG + Chr1:81212040-81212059 MsG0180004685.01.T01:CDS 30.0%
ATGAAAACAAGTTTAACCTG+TGG - Chr1:81212682-81212701 None:intergenic 30.0%
ATTAGCAAGTACCTCTAATA+AGG - Chr1:81213458-81213477 None:intergenic 30.0%
TCATGTTGAATACTGTCATT+AGG + Chr1:81211326-81211345 MsG0180004685.01.T01:intron 30.0%
TCATTTATTGTGAAGAAGAG+TGG - Chr1:81213410-81213429 None:intergenic 30.0%
TGCACTATGATGCTTAATAT+TGG + Chr1:81211518-81211537 MsG0180004685.01.T01:intron 30.0%
TTAGGACAAAAGACAAAGTT+AGG - Chr1:81213218-81213237 None:intergenic 30.0%
! AATGGTAAGAAAGTTCTAGT+TGG + Chr1:81212486-81212505 MsG0180004685.01.T01:intron 30.0%
! ACTCACCCAGATAATTTTAT+TGG - Chr1:81212191-81212210 None:intergenic 30.0%
! CAAAGTTTGATAACATGATG+TGG + Chr1:81211720-81211739 MsG0180004685.01.T01:intron 30.0%
! TAATAGAGTACTTCAAGATC+AGG - Chr1:81212718-81212737 None:intergenic 30.0%
! TTCATTTTCAAAAACTTGCG+AGG + Chr1:81213032-81213051 MsG0180004685.01.T01:intron 30.0%
! TTGTTTTCATCAACCCTTTT+AGG + Chr1:81212692-81212711 MsG0180004685.01.T01:intron 30.0%
! TTTTACCGAGACATACTTAT+CGG - Chr1:81213766-81213785 None:intergenic 30.0%
! TTTTATGGAACTGTCACTAA+AGG - Chr1:81212742-81212761 None:intergenic 30.0%
!!! TTTTGAGTTCTTGAGTGAAA+TGG + Chr1:81211380-81211399 MsG0180004685.01.T01:intron 30.0%
AAAAAGAAGGGTTTGCCAAA+GGG + Chr1:81212052-81212071 MsG0180004685.01.T01:CDS 35.0%
AACCAAATGATGAAGGAACA+AGG - Chr1:81211151-81211170 None:intergenic 35.0%
AAGATCACTGGAATTTGACA+TGG + Chr1:81212428-81212447 MsG0180004685.01.T01:intron 35.0%
AATACTGTCATTAGGATGTC+TGG + Chr1:81211334-81211353 MsG0180004685.01.T01:intron 35.0%
AATCAATCCTATTCCTTACC+AGG - Chr1:81210998-81211017 None:intergenic 35.0%
AATTGGATCACAATTGTTGC+AGG + Chr1:81211611-81211630 MsG0180004685.01.T01:intron 35.0%
ACAGGTTTCAAGAAATTCCA+TGG + Chr1:81211977-81211996 MsG0180004685.01.T01:CDS 35.0%
ACTTGAAGATTCAGATTCCT+TGG - Chr1:81212167-81212186 None:intergenic 35.0%
ATACTGTCATTAGGATGTCT+GGG + Chr1:81211335-81211354 MsG0180004685.01.T01:intron 35.0%
ATGAAAACATAATTGGGTGG+TGG - Chr1:81211310-81211329 None:intergenic 35.0%
ATTGGATCACAATTGTTGCA+GGG + Chr1:81211612-81211631 MsG0180004685.01.T01:intron 35.0%
CAACAATTGTGATCATGTTG+TGG + Chr1:81211579-81211598 MsG0180004685.01.T01:intron 35.0%
CTACACACAAAGTATGACTA+AGG - Chr1:81212245-81212264 None:intergenic 35.0%
CTTAATATTGGTGAGAATCG+CGG + Chr1:81211530-81211549 MsG0180004685.01.T01:intron 35.0%
CTTAGTCATACTTTGTGTGT+AGG + Chr1:81212243-81212262 MsG0180004685.01.T01:intron 35.0%
GACGGAATATGTGAAAAAGA+AGG + Chr1:81212039-81212058 MsG0180004685.01.T01:CDS 35.0%
TGTAGTATTCTTGATACTGC+TGG + Chr1:81212091-81212110 MsG0180004685.01.T01:CDS 35.0%
TTATTCACAATTGTCTCCGT+TGG - Chr1:81212016-81212035 None:intergenic 35.0%
TTCAGATTCCTTGGCAATAA+CGG - Chr1:81212158-81212177 None:intergenic 35.0%
TTCCTTGTTCCTTCATCATT+TGG + Chr1:81211146-81211165 MsG0180004685.01.T01:intron 35.0%
! ATGTTTGTTCAGCTGCATTT+TGG + Chr1:81212568-81212587 MsG0180004685.01.T01:intron 35.0%
! ATTTGCTGCTTCCTTATTAG+AGG + Chr1:81213444-81213463 MsG0180004685.01.T01:CDS 35.0%
! CACAAAGTTTCTAGATACCA+AGG - Chr1:81213011-81213030 None:intergenic 35.0%
! GATGTCTGGGATTACAAAAT+TGG + Chr1:81211348-81211367 MsG0180004685.01.T01:intron 35.0%
! TGTTTGTTCAGCTGCATTTT+GGG + Chr1:81212569-81212588 MsG0180004685.01.T01:intron 35.0%
! TTTTAGCCCTTAAAATGCTG+AGG + Chr1:81213631-81213650 MsG0180004685.01.T01:three_prime_UTR 35.0%
! TTTTCGATTATCTGAGTCCT+TGG + Chr1:81212991-81213010 MsG0180004685.01.T01:intron 35.0%
!!! TGTTCCATGTTTTTGAGATG+CGG + Chr1:81213181-81213200 MsG0180004685.01.T01:intron 35.0%
AACAAGTCTTTGCCTCTTGA+TGG + Chr1:81213075-81213094 MsG0180004685.01.T01:intron 40.0%
AACCAAACCTTCTTATCCAG+TGG - Chr1:81211811-81211830 None:intergenic 40.0%
ACAACCGCATCTCAAAAACA+TGG - Chr1:81213188-81213207 None:intergenic 40.0%
ACCAGAACAATTACATGAGC+TGG - Chr1:81213492-81213511 None:intergenic 40.0%
ACCTCCCGTATTCTTGAAAT+CGG - Chr1:81212804-81212823 None:intergenic 40.0%
ACTTCAAGATCAGGCCTAAA+AGG - Chr1:81212709-81212728 None:intergenic 40.0%
AGGAAGCAGCAAATTGCATT+TGG - Chr1:81213438-81213457 None:intergenic 40.0%
AGGGTAGTCTGCATAAAGTT+AGG - Chr1:81213236-81213255 None:intergenic 40.0%
AGGTGCAAGTGCAAAGATAT+TGG + Chr1:81213106-81213125 MsG0180004685.01.T01:intron 40.0%
ATCACGCAACCAAATGATGA+AGG - Chr1:81211158-81211177 None:intergenic 40.0%
ATCATAACCTTTCGTTGCCA+AGG - Chr1:81213282-81213301 None:intergenic 40.0%
ATGGCCCGATTTCAAGAATA+CGG + Chr1:81212796-81212815 MsG0180004685.01.T01:CDS 40.0%
ATTCTTGATACTGCTGGACA+AGG + Chr1:81212097-81212116 MsG0180004685.01.T01:CDS 40.0%
CAAGAATACGGGAGGTAAAT+TGG + Chr1:81212808-81212827 MsG0180004685.01.T01:CDS 40.0%
CCAGAACAATTACATGAGCT+GGG - Chr1:81213491-81213510 None:intergenic 40.0%
CTTCAAGATCAGGCCTAAAA+GGG - Chr1:81212708-81212727 None:intergenic 40.0%
GAAAAAGAAGGGTTTGCCAA+AGG + Chr1:81212051-81212070 MsG0180004685.01.T01:CDS 40.0%
GGAAGCAGCAAATTGCATTT+GGG - Chr1:81213437-81213456 None:intergenic 40.0%
TAACTGCGACTAAAGATCAC+TGG + Chr1:81212416-81212435 MsG0180004685.01.T01:intron 40.0%
TCCATTTCCTGGTAAGGAAT+AGG + Chr1:81210988-81211007 MsG0180004685.01.T01:five_prime_UTR 40.0%
TCCTATTCCTTACCAGGAAA+TGG - Chr1:81210992-81211011 None:intergenic 40.0%
TGGAGTTTCAGAGAATCCAA+CGG + Chr1:81211997-81212016 MsG0180004685.01.T01:CDS 40.0%
TGTAGCCGATAAGTATGTCT+CGG + Chr1:81213758-81213777 MsG0180004685.01.T01:three_prime_UTR 40.0%
TGTTGTGGTGATATCGCAAT+TGG + Chr1:81211594-81211613 MsG0180004685.01.T01:intron 40.0%
TTGCCAAAGGGTTTAGCTAT+CGG + Chr1:81212064-81212083 MsG0180004685.01.T01:CDS 40.0%
! AGCTTGCCTCAGCATTTTAA+GGG - Chr1:81213640-81213659 None:intergenic 40.0%
! GTAATGACATCTGATGATGC+AGG + Chr1:81213302-81213321 MsG0180004685.01.T01:CDS 40.0%
! GTTTGTTCAGCTGCATTTTG+GGG + Chr1:81212570-81212589 MsG0180004685.01.T01:intron 40.0%
! TGTTGTCATGCTTCTGAGAA+TGG + Chr1:81213511-81213530 MsG0180004685.01.T01:three_prime_UTR 40.0%
!! TGTATATGATGATGGTCCAC+TGG + Chr1:81211792-81211811 MsG0180004685.01.T01:intron 40.0%
!! TTGGATTCTCTGAAACTCCA+TGG - Chr1:81211997-81212016 None:intergenic 40.0%
AAGTCTTTGCCTCTTGATGG+AGG + Chr1:81213078-81213097 MsG0180004685.01.T01:intron 45.0%
ACCTATATGCCTCCATCAAG+AGG - Chr1:81213090-81213109 None:intergenic 45.0%
ACTCACAAAGTAGGGATCCT+TGG - Chr1:81212216-81212235 None:intergenic 45.0%
ACTGTTGTTACAGCAGCTGA+AGG - Chr1:81211950-81211969 None:intergenic 45.0%
AGAACGTCAAGCTGTCAATG+AGG + Chr1:81212621-81212640 MsG0180004685.01.T01:CDS 45.0%
AGGATCCCTACTTTGTGAGT+AGG + Chr1:81212216-81212235 MsG0180004685.01.T01:intron 45.0%
ATCCCATTTCATCAGGGCAA+CGG - Chr1:81212654-81212673 None:intergenic 45.0%
ATTAGACCATCATCCGCAGA+TGG + Chr1:81211913-81211932 MsG0180004685.01.T01:exon 45.0%
ATTATCTGGGTGAGTTGCCA+AGG + Chr1:81212196-81212215 MsG0180004685.01.T01:intron 45.0%
CCCAGCTCATGTAATTGTTC+TGG + Chr1:81213488-81213507 MsG0180004685.01.T01:three_prime_UTR 45.0%
CCTCCCGTATTCTTGAAATC+GGG - Chr1:81212803-81212822 None:intergenic 45.0%
GATTGCAATGTCTGGTACAG+CGG - Chr1:81212133-81212152 None:intergenic 45.0%
GCTTCCATCCCATTTCATCA+GGG - Chr1:81212660-81212679 None:intergenic 45.0%
GGATCCCTACTTTGTGAGTA+GGG + Chr1:81212217-81212236 MsG0180004685.01.T01:intron 45.0%
GTCCACTGGATAAGAAGGTT+TGG + Chr1:81211806-81211825 MsG0180004685.01.T01:intron 45.0%
GTTCCCATTGTTCCTTCAGA+TGG + Chr1:81212777-81212796 MsG0180004685.01.T01:CDS 45.0%
TAACGGCAGATTGCAATGTC+TGG - Chr1:81212141-81212160 None:intergenic 45.0%
TCTCTCGAGTTCGTAGTGTT+TGG + Chr1:81212865-81212884 MsG0180004685.01.T01:CDS 45.0%
TGAGATCGTGGTCCAAATTG+TGG + Chr1:81211656-81211675 MsG0180004685.01.T01:intron 45.0%
TGATGGTCCACTGGATAAGA+AGG + Chr1:81211801-81211820 MsG0180004685.01.T01:intron 45.0%
TGCCAAAGGGTTTAGCTATC+GGG + Chr1:81212065-81212084 MsG0180004685.01.T01:CDS 45.0%
TGGCCCGATTTCAAGAATAC+GGG + Chr1:81212797-81212816 MsG0180004685.01.T01:CDS 45.0%
TTAGACCATCATCCGCAGAT+GGG + Chr1:81211914-81211933 MsG0180004685.01.T01:exon 45.0%
TTCCGTTGCCCTGATGAAAT+GGG + Chr1:81212649-81212668 MsG0180004685.01.T01:CDS 45.0%
! CAGCTTGCCTCAGCATTTTA+AGG - Chr1:81213641-81213660 None:intergenic 45.0%
! CTATCATTCTCTTCGGTTCG+CGG + Chr1:81213349-81213368 MsG0180004685.01.T01:CDS 45.0%
! TGACATCTGATGATGCAGGA+AGG + Chr1:81213306-81213325 MsG0180004685.01.T01:CDS 45.0%
!! GTTTTGATGCGATGAGATCG+TGG + Chr1:81211644-81211663 MsG0180004685.01.T01:intron 45.0%
!!! CTGCATTTTGGGGTTAACAG+TGG + Chr1:81212580-81212599 MsG0180004685.01.T01:CDS 45.0%
!!! TTTTATAATATCATTTATTT+TGG + Chr1:81212914-81212933 MsG0180004685.01.T01:intron 5.0%
ACCAAGACCTTGGCAACGAA+AGG + Chr1:81213272-81213291 MsG0180004685.01.T01:CDS 50.0%
ACCTTTCGTTGCCAAGGTCT+TGG - Chr1:81213276-81213295 None:intergenic 50.0%
AGGCTCCCTACTCACAAAGT+AGG - Chr1:81212225-81212244 None:intergenic 50.0%
ATGTCTGGTACAGCGGAACA+AGG - Chr1:81212126-81212145 None:intergenic 50.0%
CATTTCATCAGGGCAACGGA+AGG - Chr1:81212650-81212669 None:intergenic 50.0%
CCCGATTTCAAGAATACGGG+AGG + Chr1:81212800-81212819 MsG0180004685.01.T01:CDS 50.0%
CGGTCAACTGACGCTGATAT+GGG + Chr1:81211550-81211569 MsG0180004685.01.T01:intron 50.0%
CTCCCGATAGCTAAACCCTT+TGG - Chr1:81212070-81212089 None:intergenic 50.0%
CTGAGGCAAGCTGACATCTT+AGG + Chr1:81213648-81213667 MsG0180004685.01.T01:three_prime_UTR 50.0%
CTTCCGTTGCCCTGATGAAA+TGG + Chr1:81212648-81212667 MsG0180004685.01.T01:CDS 50.0%
CTTGAAATCGGGCCATCTGA+AGG - Chr1:81212792-81212811 None:intergenic 50.0%
GCAATCTGCCGTTATTGCCA+AGG + Chr1:81212147-81212166 MsG0180004685.01.T01:CDS 50.0%
GGCTCCCTACTCACAAAGTA+GGG - Chr1:81212224-81212243 None:intergenic 50.0%
GGCTTCCATCCCATTTCATC+AGG - Chr1:81212661-81212680 None:intergenic 50.0%
GGGCCATCTGAAGGAACAAT+GGG - Chr1:81212783-81212802 None:intergenic 50.0%
GGGTTAACAGTGGTGCAACA+AGG + Chr1:81212590-81212609 MsG0180004685.01.T01:CDS 50.0%
GTTGCCCTGATGAAATGGGA+TGG + Chr1:81212653-81212672 MsG0180004685.01.T01:CDS 50.0%
TACAGCAGCTGAAGGACCTT+CGG - Chr1:81211942-81211961 None:intergenic 50.0%
TAGACCATCATCCGCAGATG+GGG + Chr1:81211915-81211934 MsG0180004685.01.T01:exon 50.0%
TATGCAGACTACCCTTCCTG+TGG + Chr1:81213241-81213260 MsG0180004685.01.T01:intron 50.0%
TGAAATGGGATGGAAGCCAC+AGG + Chr1:81212663-81212682 MsG0180004685.01.T01:CDS 50.0%
TGTCTGGTACAGCGGAACAA+GGG - Chr1:81212125-81212144 None:intergenic 50.0%
! GCCTCTTGATGGAGGCATAT+AGG + Chr1:81213086-81213105 MsG0180004685.01.T01:intron 50.0%
CGGGAGGTAAATTGGATCGC+TGG + Chr1:81212816-81212835 MsG0180004685.01.T01:CDS 55.0%
CGGGCCATCTGAAGGAACAA+TGG - Chr1:81212784-81212803 None:intergenic 55.0%
GCACGTCTGCGACCACAATT+TGG - Chr1:81211671-81211690 None:intergenic 55.0%
GCGGTCAACTGACGCTGATA+TGG + Chr1:81211549-81211568 MsG0180004685.01.T01:intron 55.0%
GGAGCAGATGACCAAGACCT+TGG + Chr1:81213262-81213281 MsG0180004685.01.T01:CDS 55.0%
GGTCATCTGCTCCACAGGAA+GGG - Chr1:81213255-81213274 None:intergenic 55.0%
GTCTTGGTCATCTGCTCCAC+AGG - Chr1:81213260-81213279 None:intergenic 55.0%
TGGTCATCTGCTCCACAGGA+AGG - Chr1:81213256-81213275 None:intergenic 55.0%
! CTTCGGTTCGCGGAGCAGAA+CGG + Chr1:81213359-81213378 MsG0180004685.01.T01:CDS 60.0%
CATCCGCAGATGGGGTCCGA+AGG + Chr1:81211923-81211942 MsG0180004685.01.T01:exon 65.0%
CGGACCCCATCTGCGGATGA+TGG - Chr1:81211922-81211941 None:intergenic 65.0%
GGACCTTCGGACCCCATCTG+CGG - Chr1:81211929-81211948 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 81210986 81213829 81210986 ID=MsG0180004685.01;Name=MsG0180004685.01
Chr1 mRNA 81210986 81213829 81210986 ID=MsG0180004685.01.T01;Parent=MsG0180004685.01;Name=MsG0180004685.01.T01;_AED=0.31;_eAED=0.31;_QI=209|0.75|0.8|1|0.75|0.6|5|366|242
Chr1 exon 81210986 81211135 81210986 ID=MsG0180004685.01.T01:exon:34354;Parent=MsG0180004685.01.T01
Chr1 exon 81211873 81212204 81211873 ID=MsG0180004685.01.T01:exon:34355;Parent=MsG0180004685.01.T01
Chr1 exon 81212580 81212684 81212580 ID=MsG0180004685.01.T01:exon:34356;Parent=MsG0180004685.01.T01
Chr1 exon 81212774 81212908 81212774 ID=MsG0180004685.01.T01:exon:34357;Parent=MsG0180004685.01.T01
Chr1 exon 81213248 81213829 81213248 ID=MsG0180004685.01.T01:exon:34358;Parent=MsG0180004685.01.T01
Chr1 five_prime_UTR 81210986 81211135 81210986 ID=MsG0180004685.01.T01:five_prime_utr;Parent=MsG0180004685.01.T01
Chr1 five_prime_UTR 81211873 81211931 81211873 ID=MsG0180004685.01.T01:five_prime_utr;Parent=MsG0180004685.01.T01
Chr1 CDS 81211932 81212204 81211932 ID=MsG0180004685.01.T01:cds;Parent=MsG0180004685.01.T01
Chr1 CDS 81212580 81212684 81212580 ID=MsG0180004685.01.T01:cds;Parent=MsG0180004685.01.T01
Chr1 CDS 81212774 81212908 81212774 ID=MsG0180004685.01.T01:cds;Parent=MsG0180004685.01.T01
Chr1 CDS 81213248 81213463 81213248 ID=MsG0180004685.01.T01:cds;Parent=MsG0180004685.01.T01
Chr1 three_prime_UTR 81213464 81213829 81213464 ID=MsG0180004685.01.T01:three_prime_utr;Parent=MsG0180004685.01.T01
Gene Sequence

>MsG0180004685.01.T01

ATGGGGTCCGAAGGTCCTTCAGCTGCTGTAACAACAGTTTATATAACAGGTTTCAAGAAATTCCATGGAGTTTCAGAGAATCCAACGGAGACAATTGTGAATAATTTGACGGAATATGTGAAAAAGAAGGGTTTGCCAAAGGGTTTAGCTATCGGGAGCTGTAGTATTCTTGATACTGCTGGACAAGGAGCCCTTGTTCCGCTGTACCAGACATTGCAATCTGCCGTTATTGCCAAGGAATCTGAATCTTCAAGTTCCAATAAAATTATCTGGCTGCATTTTGGGGTTAACAGTGGTGCAACAAGGTTCGCTATAGAACGTCAAGCTGTCAATGAGGCTACCTTCCGTTGCCCTGATGAAATGGGATGGAAGCCACAGAAAGTTCCCATTGTTCCTTCAGATGGCCCGATTTCAAGAATACGGGAGGTAAATTGGATCGCTGGTTTTCTTTTGCTTCGTAAATCAATGTTCTCTCGAGTTCGTAGTGTTTGGTACAAATATGAAAATGTTGAGACTACCCTTCCTGTGGAGCAGATGACCAAGACCTTGGCAACGAAAGGTTATGATGTAATGACATCTGATGATGCAGGAAGGTTTGTGTGCAATTATGTTTACTATCATTCTCTTCGGTTCGCGGAGCAGAACGGAAACAAATCACTTTTCGTGCATGTGCCACTCTTCTTCACAATAAATGAGAAACCCAAATGCAATTTGCTGCTTCCTTATTAG
Protein sequence

>MsG0180004685.01.T01

MGSEGPSAAVTTVYITGFKKFHGVSENPTETIVNNLTEYVKKKGLPKGLAIGSCSILDTAGQGALVPLYQTLQSAVIAKESESSSSNKIIWLHFGVNSGATRFAIERQAVNEATFRCPDEMGWKPQKVPIVPSDGPISRIREVNWIAGFLLLRKSMFSRVRSVWYKYENVETTLPVEQMTKTLATKGYDVMTSDDAGRFVCNYVYYHSLRFAEQNGNKSLFVHVPLFFTINEKPKCNLLLPY*