Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004728.01.T01 | XP_013469117.1 | 94.521 | 219 | 11 | 1 | 1 | 218 | 1 | 219 | 1.00E-145 | 415 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004728.01.T01 | Q9M263 | 69.091 | 220 | 64 | 3 | 1 | 217 | 1 | 219 | 6.14E-104 | 302 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004728.01.T01 | A0A072VYK0 | 94.521 | 219 | 11 | 1 | 1 | 218 | 1 | 219 | 4.79e-146 | 415 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000260.01 | MsG0180004728.01 | 0.846039 | 2.788906e-59 | 4.738954e-56 |
| MsG0180004728.01 | MsG0380016193.01 | 0.806987 | 6.298099e-50 | 3.467049e-47 |
| MsG0180004728.01 | MsG0480018596.01 | 0.860841 | 1.558985e-63 | 4.320836e-60 |
| MsG0180004728.01 | MsG0680031666.01 | 0.812570 | 3.966230e-51 | 2.530619e-48 |
| MsG0180004728.01 | MsG0780040906.01 | 0.843016 | 1.816020e-58 | 2.803418e-55 |
| MsG0180004728.01 | MsG0780040921.01 | 0.851435 | 8.890165e-61 | 1.799349e-57 |
| MsG0180004728.01 | MsG0880047577.01 | 0.824944 | 6.134072e-54 | 5.513271e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004728.01.T01 | MTR_1g085500 | 94.521 | 219 | 11 | 1 | 1 | 218 | 1 | 219 | 1.22e-149 | 415 |
| MsG0180004728.01.T01 | MTR_1g085140 | 70.500 | 200 | 58 | 1 | 19 | 217 | 20 | 219 | 8.44e-98 | 283 |
| MsG0180004728.01.T01 | MTR_7g111240 | 69.898 | 196 | 58 | 1 | 22 | 216 | 19 | 214 | 3.90e-93 | 271 |
| MsG0180004728.01.T01 | MTR_7g111240 | 69.744 | 195 | 57 | 1 | 22 | 216 | 19 | 211 | 1.63e-92 | 270 |
| MsG0180004728.01.T01 | MTR_1g079490 | 45.540 | 213 | 109 | 3 | 6 | 212 | 5 | 216 | 4.14e-58 | 183 |
| MsG0180004728.01.T01 | MTR_2g019250 | 47.115 | 208 | 102 | 4 | 11 | 212 | 10 | 215 | 2.26e-57 | 181 |
| MsG0180004728.01.T01 | MTR_4g017040 | 46.635 | 208 | 103 | 4 | 11 | 212 | 10 | 215 | 2.39e-57 | 181 |
| MsG0180004728.01.T01 | MTR_5g046410 | 46.635 | 208 | 103 | 4 | 11 | 212 | 10 | 215 | 3.53e-57 | 181 |
| MsG0180004728.01.T01 | MTR_5g046430 | 46.635 | 208 | 103 | 4 | 11 | 212 | 10 | 215 | 3.53e-57 | 181 |
| MsG0180004728.01.T01 | MTR_4g017030 | 46.635 | 208 | 103 | 4 | 11 | 212 | 10 | 215 | 3.53e-57 | 181 |
| MsG0180004728.01.T01 | MTR_4g017050 | 46.635 | 208 | 103 | 4 | 11 | 212 | 10 | 215 | 3.69e-57 | 181 |
| MsG0180004728.01.T01 | MTR_6g005340 | 47.761 | 201 | 98 | 3 | 17 | 212 | 16 | 214 | 9.21e-57 | 179 |
| MsG0180004728.01.T01 | MTR_6g005360 | 47.761 | 201 | 98 | 3 | 17 | 212 | 16 | 214 | 1.41e-56 | 179 |
| MsG0180004728.01.T01 | MTR_6g005310 | 47.761 | 201 | 98 | 3 | 17 | 212 | 16 | 214 | 3.66e-56 | 178 |
| MsG0180004728.01.T01 | MTR_6g005330 | 46.445 | 211 | 105 | 4 | 8 | 212 | 6 | 214 | 4.75e-56 | 177 |
| MsG0180004728.01.T01 | MTR_2g030855 | 46.429 | 196 | 99 | 3 | 20 | 212 | 19 | 211 | 8.06e-56 | 177 |
| MsG0180004728.01.T01 | MTR_1g023770 | 43.137 | 204 | 109 | 2 | 11 | 212 | 10 | 208 | 1.56e-55 | 176 |
| MsG0180004728.01.T01 | MTR_1g112200 | 42.584 | 209 | 114 | 3 | 7 | 215 | 7 | 209 | 1.56e-54 | 173 |
| MsG0180004728.01.T01 | MTR_6g005380 | 48.020 | 202 | 96 | 4 | 8 | 202 | 6 | 205 | 2.11e-54 | 173 |
| MsG0180004728.01.T01 | MTR_2g031270 | 44.724 | 199 | 103 | 4 | 18 | 212 | 17 | 212 | 2.52e-52 | 168 |
| MsG0180004728.01.T01 | MTR_6g005350 | 42.654 | 211 | 112 | 5 | 8 | 212 | 6 | 213 | 5.80e-50 | 162 |
| MsG0180004728.01.T01 | MTR_5g036570 | 43.655 | 197 | 105 | 3 | 22 | 214 | 26 | 220 | 5.76e-48 | 157 |
| MsG0180004728.01.T01 | MTR_1g060120 | 37.104 | 221 | 127 | 4 | 6 | 217 | 8 | 225 | 2.46e-47 | 155 |
| MsG0180004728.01.T01 | MTR_2g030895 | 44.444 | 171 | 92 | 2 | 45 | 212 | 7 | 177 | 8.71e-46 | 150 |
| MsG0180004728.01.T01 | MTR_2g030865 | 44.444 | 171 | 92 | 2 | 45 | 212 | 7 | 177 | 4.02e-45 | 148 |
| MsG0180004728.01.T01 | MTR_8g007300 | 43.243 | 185 | 100 | 3 | 6 | 187 | 10 | 192 | 2.29e-40 | 137 |
| MsG0180004728.01.T01 | MTR_8g020590 | 38.919 | 185 | 108 | 2 | 8 | 191 | 4 | 184 | 8.34e-39 | 133 |
| MsG0180004728.01.T01 | MTR_2g086640 | 40.102 | 197 | 95 | 6 | 19 | 210 | 22 | 200 | 4.96e-38 | 131 |
| MsG0180004728.01.T01 | MTR_2g086620 | 40.102 | 197 | 95 | 6 | 19 | 210 | 23 | 201 | 8.18e-38 | 130 |
| MsG0180004728.01.T01 | MTR_2g086630 | 40.102 | 197 | 95 | 6 | 19 | 210 | 23 | 201 | 1.05e-37 | 130 |
| MsG0180004728.01.T01 | MTR_8g020650 | 35.938 | 192 | 118 | 4 | 26 | 216 | 21 | 208 | 1.18e-36 | 127 |
| MsG0180004728.01.T01 | MTR_8g020630 | 35.176 | 199 | 124 | 4 | 19 | 216 | 14 | 208 | 1.62e-36 | 127 |
| MsG0180004728.01.T01 | MTR_2g036120 | 37.824 | 193 | 115 | 4 | 26 | 217 | 21 | 209 | 1.39e-35 | 125 |
| MsG0180004728.01.T01 | MTR_3g096330 | 38.350 | 206 | 120 | 4 | 9 | 209 | 561 | 764 | 1.43e-35 | 133 |
| MsG0180004728.01.T01 | MTR_2g031300 | 41.250 | 160 | 72 | 2 | 45 | 202 | 7 | 146 | 1.99e-35 | 123 |
| MsG0180004728.01.T01 | MTR_2g045100 | 37.500 | 208 | 125 | 4 | 8 | 214 | 3 | 206 | 6.14e-35 | 123 |
| MsG0180004728.01.T01 | MTR_3g019680 | 37.895 | 190 | 113 | 4 | 26 | 214 | 20 | 205 | 8.17e-35 | 123 |
| MsG0180004728.01.T01 | MTR_3g019650 | 36.842 | 190 | 115 | 4 | 26 | 214 | 20 | 205 | 8.53e-35 | 123 |
| MsG0180004728.01.T01 | MTR_4g010340 | 39.614 | 207 | 108 | 8 | 9 | 212 | 8 | 200 | 1.06e-34 | 122 |
| MsG0180004728.01.T01 | MTR_3g019710 | 37.000 | 200 | 115 | 5 | 19 | 214 | 14 | 206 | 1.45e-34 | 122 |
| MsG0180004728.01.T01 | MTR_4g052770 | 43.284 | 134 | 73 | 2 | 78 | 210 | 71 | 202 | 1.55e-34 | 122 |
| MsG0180004728.01.T01 | MTR_4g052780 | 43.284 | 134 | 73 | 2 | 78 | 210 | 71 | 202 | 2.18e-34 | 122 |
| MsG0180004728.01.T01 | MTR_5g031460 | 34.123 | 211 | 128 | 5 | 8 | 214 | 3 | 206 | 7.03e-34 | 120 |
| MsG0180004728.01.T01 | MTR_8g020670 | 32.381 | 210 | 133 | 5 | 8 | 216 | 7 | 208 | 1.14e-33 | 120 |
| MsG0180004728.01.T01 | MTR_2g041550 | 37.297 | 185 | 109 | 3 | 29 | 210 | 23 | 203 | 1.89e-33 | 119 |
| MsG0180004728.01.T01 | MTR_2g044040 | 35.096 | 208 | 126 | 5 | 8 | 214 | 7 | 206 | 1.13e-32 | 117 |
| MsG0180004728.01.T01 | MTR_2g041570 | 34.300 | 207 | 128 | 3 | 6 | 211 | 5 | 204 | 1.39e-32 | 117 |
| MsG0180004728.01.T01 | MTR_2g041580 | 35.437 | 206 | 125 | 4 | 6 | 210 | 5 | 203 | 2.13e-32 | 117 |
| MsG0180004728.01.T01 | MTR_0284s0010 | 35.533 | 197 | 122 | 4 | 19 | 214 | 14 | 206 | 3.25e-31 | 114 |
| MsG0180004728.01.T01 | MTR_7g011340 | 37.037 | 189 | 114 | 5 | 27 | 214 | 22 | 206 | 7.57e-31 | 112 |
| MsG0180004728.01.T01 | MTR_8g020750 | 36.275 | 204 | 120 | 4 | 9 | 210 | 10 | 205 | 2.82e-30 | 111 |
| MsG0180004728.01.T01 | MTR_8g020770 | 41.420 | 169 | 89 | 5 | 8 | 172 | 7 | 169 | 4.18e-30 | 111 |
| MsG0180004728.01.T01 | MTR_8g020763 | 36.047 | 172 | 107 | 3 | 41 | 212 | 17 | 185 | 5.74e-29 | 107 |
| MsG0180004728.01.T01 | MTR_8g020610 | 32.524 | 206 | 130 | 4 | 6 | 210 | 3 | 200 | 7.81e-28 | 105 |
| MsG0180004728.01.T01 | MTR_2g019780 | 31.754 | 211 | 134 | 6 | 5 | 212 | 4 | 207 | 6.03e-27 | 102 |
| MsG0180004728.01.T01 | MTR_8g020760 | 34.518 | 197 | 120 | 6 | 19 | 212 | 16 | 206 | 2.02e-26 | 101 |
| MsG0180004728.01.T01 | MTR_3g019810 | 34.146 | 205 | 122 | 7 | 8 | 210 | 109 | 302 | 1.86e-25 | 101 |
| MsG0180004728.01.T01 | MTR_3g073170 | 33.333 | 186 | 115 | 5 | 29 | 212 | 27 | 205 | 3.14e-25 | 98.6 |
| MsG0180004728.01.T01 | MTR_4g027415 | 43.089 | 123 | 67 | 3 | 27 | 148 | 22 | 142 | 3.12e-22 | 88.6 |
| MsG0180004728.01.T01 | MTR_3g019610 | 42.308 | 104 | 58 | 2 | 8 | 110 | 3 | 105 | 4.80e-16 | 71.6 |
| MsG0180004728.01.T01 | MTR_2g072560 | 40.351 | 114 | 66 | 2 | 8 | 120 | 3 | 115 | 8.32e-15 | 69.3 |
| MsG0180004728.01.T01 | MTR_2g030915 | 44.776 | 67 | 25 | 2 | 93 | 159 | 1 | 55 | 1.03e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004728.01.T01 | AT3G62020 | 69.091 | 220 | 64 | 3 | 1 | 217 | 1 | 219 | 6.26e-105 | 302 |
| MsG0180004728.01.T01 | AT1G02335 | 64.455 | 211 | 74 | 1 | 8 | 217 | 9 | 219 | 6.53e-99 | 286 |
| MsG0180004728.01.T01 | AT1G09560 | 70.707 | 198 | 57 | 1 | 21 | 217 | 22 | 219 | 4.34e-98 | 284 |
| MsG0180004728.01.T01 | AT3G62020 | 74.444 | 180 | 43 | 2 | 40 | 217 | 12 | 190 | 5.64e-96 | 278 |
| MsG0180004728.01.T01 | AT5G26700 | 59.278 | 194 | 75 | 2 | 25 | 217 | 23 | 213 | 4.61e-84 | 249 |
| MsG0180004728.01.T01 | AT3G05930 | 53.425 | 219 | 100 | 2 | 1 | 217 | 1 | 219 | 6.05e-76 | 228 |
| MsG0180004728.01.T01 | AT1G18980 | 47.368 | 209 | 107 | 2 | 7 | 214 | 13 | 219 | 8.63e-65 | 200 |
| MsG0180004728.01.T01 | AT1G18970 | 47.847 | 209 | 106 | 2 | 7 | 214 | 13 | 219 | 3.15e-64 | 199 |
| MsG0180004728.01.T01 | AT5G38930 | 49.246 | 199 | 96 | 2 | 19 | 212 | 22 | 220 | 1.78e-60 | 189 |
| MsG0180004728.01.T01 | AT5G38940 | 48.980 | 196 | 95 | 2 | 22 | 212 | 25 | 220 | 3.53e-60 | 188 |
| MsG0180004728.01.T01 | AT5G38940 | 48.980 | 196 | 95 | 2 | 22 | 212 | 49 | 244 | 5.36e-60 | 189 |
| MsG0180004728.01.T01 | AT5G38910 | 48.558 | 208 | 102 | 3 | 11 | 213 | 12 | 219 | 5.90e-60 | 187 |
| MsG0180004728.01.T01 | AT5G39160 | 45.024 | 211 | 111 | 2 | 9 | 214 | 10 | 220 | 9.53e-60 | 187 |
| MsG0180004728.01.T01 | AT5G39190 | 45.024 | 211 | 111 | 2 | 9 | 214 | 10 | 220 | 1.42e-59 | 187 |
| MsG0180004728.01.T01 | AT5G39130 | 45.024 | 211 | 111 | 2 | 9 | 214 | 10 | 220 | 1.83e-59 | 186 |
| MsG0180004728.01.T01 | AT3G04200 | 45.283 | 212 | 109 | 3 | 11 | 215 | 13 | 224 | 3.92e-59 | 186 |
| MsG0180004728.01.T01 | AT4G14630 | 50.000 | 198 | 93 | 3 | 21 | 212 | 24 | 221 | 4.09e-59 | 186 |
| MsG0180004728.01.T01 | AT5G39110 | 46.602 | 206 | 104 | 2 | 13 | 212 | 14 | 219 | 4.56e-59 | 186 |
| MsG0180004728.01.T01 | AT5G38960 | 48.500 | 200 | 97 | 3 | 21 | 214 | 24 | 223 | 6.31e-59 | 185 |
| MsG0180004728.01.T01 | AT5G39150 | 45.498 | 211 | 109 | 2 | 8 | 212 | 8 | 218 | 6.34e-59 | 185 |
| MsG0180004728.01.T01 | AT5G39180 | 45.498 | 211 | 109 | 2 | 8 | 212 | 8 | 218 | 1.82e-58 | 184 |
| MsG0180004728.01.T01 | AT5G39120 | 47.475 | 198 | 98 | 2 | 21 | 212 | 21 | 218 | 1.82e-58 | 184 |
| MsG0180004728.01.T01 | AT3G05950 | 47.143 | 210 | 104 | 3 | 12 | 214 | 13 | 222 | 2.15e-58 | 184 |
| MsG0180004728.01.T01 | AT5G39160 | 44.498 | 209 | 111 | 2 | 9 | 214 | 10 | 216 | 2.38e-58 | 183 |
| MsG0180004728.01.T01 | AT5G39160 | 40.670 | 209 | 101 | 2 | 9 | 214 | 10 | 198 | 1.23e-50 | 163 |
| MsG0180004728.01.T01 | AT3G04170 | 43.192 | 213 | 111 | 4 | 11 | 216 | 12 | 221 | 1.61e-50 | 164 |
| MsG0180004728.01.T01 | AT5G39190 | 41.063 | 207 | 99 | 2 | 9 | 212 | 10 | 196 | 2.01e-50 | 162 |
| MsG0180004728.01.T01 | AT3G04150 | 41.063 | 207 | 115 | 3 | 11 | 210 | 12 | 218 | 4.95e-50 | 162 |
| MsG0180004728.01.T01 | AT3G04180 | 42.574 | 202 | 105 | 4 | 17 | 210 | 20 | 218 | 1.10e-48 | 159 |
| MsG0180004728.01.T01 | AT3G04150 | 39.091 | 220 | 114 | 3 | 11 | 210 | 12 | 231 | 1.83e-48 | 159 |
| MsG0180004728.01.T01 | AT3G04190 | 41.709 | 199 | 111 | 2 | 17 | 210 | 20 | 218 | 1.46e-47 | 156 |
| MsG0180004728.01.T01 | AT3G10080 | 40.394 | 203 | 112 | 3 | 19 | 215 | 22 | 221 | 1.76e-47 | 156 |
| MsG0180004728.01.T01 | AT3G10080 | 40.394 | 203 | 112 | 3 | 19 | 215 | 93 | 292 | 8.95e-47 | 156 |
| MsG0180004728.01.T01 | AT1G74820 | 37.441 | 211 | 124 | 3 | 8 | 212 | 16 | 224 | 8.89e-43 | 144 |
| MsG0180004728.01.T01 | AT5G38910 | 40.976 | 205 | 83 | 3 | 11 | 212 | 12 | 181 | 2.61e-40 | 136 |
| MsG0180004728.01.T01 | AT1G72610 | 37.634 | 186 | 113 | 2 | 26 | 210 | 18 | 201 | 1.83e-39 | 135 |
| MsG0180004728.01.T01 | AT1G10460 | 38.725 | 204 | 107 | 5 | 22 | 213 | 18 | 215 | 6.52e-39 | 134 |
| MsG0180004728.01.T01 | AT5G61750 | 39.535 | 172 | 101 | 1 | 11 | 179 | 7 | 178 | 4.13e-38 | 131 |
| MsG0180004728.01.T01 | AT5G20630 | 36.275 | 204 | 123 | 3 | 8 | 210 | 7 | 204 | 1.31e-35 | 125 |
| MsG0180004728.01.T01 | AT5G38950 | 39.744 | 78 | 45 | 1 | 142 | 217 | 2 | 79 | 1.30e-12 | 62.4 |
Find 41 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTATAAATTAAGTCTTCTT+AGG | 0.231546 | 1:+81884384 | None:intergenic |
| TGATAATGCACCAACCCTTT+TGG | 0.273835 | 1:+81884585 | None:intergenic |
| CAACAACACTGTAGGATCTT+TGG | 0.279663 | 1:-81884826 | MsG0180004728.01.T01:CDS |
| TAATCATGTCACCAACAAAT+TGG | 0.290827 | 1:-81885622 | None:intergenic |
| CAAAGGTCTTGAAGTGTATC+AGG | 0.335291 | 1:+81885548 | None:intergenic |
| GTGCATTATCAGAAGAATAA+TGG | 0.349181 | 1:-81884573 | MsG0180004728.01.T01:CDS |
| TGCATTATCAGAAGAATAAT+GGG | 0.349638 | 1:-81884572 | MsG0180004728.01.T01:CDS |
| CTTTCAGATTGATACTAAAC+AGG | 0.392793 | 1:-81884433 | MsG0180004728.01.T01:CDS |
| TATGTAGGTGTTAAGGTGAA+TGG | 0.396371 | 1:-81884924 | MsG0180004728.01.T01:intron |
| ACAGTGTTGTTGACAACCTT+AGG | 0.398287 | 1:+81884837 | None:intergenic |
| CACGAGGGTGTGTGTGTGGC+GGG | 0.405545 | 1:+81884715 | None:intergenic |
| GAAATCTTTGTGTTCCCAAA+AGG | 0.405554 | 1:-81884600 | MsG0180004728.01.T01:CDS |
| TCCTGATGATGTTCTTGCTC+AGG | 0.416572 | 1:-81884457 | MsG0180004728.01.T01:CDS |
| TGTGTAGCTCTTCCATCATC+AGG | 0.427639 | 1:-81885527 | MsG0180004728.01.T01:CDS |
| TGTGGCGGGTTAAGACCATT+AGG | 0.441756 | 1:+81884729 | None:intergenic |
| AGAATAGACTACAAACCTAA+TGG | 0.465489 | 1:-81884744 | MsG0180004728.01.T01:CDS |
| GCCTGAGCAAGAACATCATC+AGG | 0.472294 | 1:+81884456 | None:intergenic |
| CAGTGTTGTTGACAACCTTA+GGG | 0.477171 | 1:+81884838 | None:intergenic |
| AAATCTTTGTGTTCCCAAAA+GGG | 0.493371 | 1:-81884599 | MsG0180004728.01.T01:CDS |
| CTTTGTGTTCCCAAAAGGGT+TGG | 0.498869 | 1:-81884595 | MsG0180004728.01.T01:CDS |
| CATTATACATTACCTGATGA+TGG | 0.506437 | 1:+81885515 | None:intergenic |
| ATTCCTGGACTCAACACATT+GGG | 0.515180 | 1:-81884780 | MsG0180004728.01.T01:CDS |
| GCACGAGGGTGTGTGTGTGG+CGG | 0.527458 | 1:+81884714 | None:intergenic |
| ACTCCCAATGTGTTGAGTCC+AGG | 0.538199 | 1:+81884777 | None:intergenic |
| TGATGGTTTAGCAAACCCTA+AGG | 0.553318 | 1:-81884853 | MsG0180004728.01.T01:CDS |
| TTGGAAGGTGAATTAGATGT+AGG | 0.564991 | 1:-81884672 | MsG0180004728.01.T01:CDS |
| AATTCCTGGACTCAACACAT+TGG | 0.570200 | 1:-81884781 | MsG0180004728.01.T01:CDS |
| TGTATCAGGATCAGAAGCGA+AGG | 0.579047 | 1:+81885562 | None:intergenic |
| AGAAGCGAAGGTAGATGAGA+TGG | 0.579500 | 1:+81885574 | None:intergenic |
| GAAACAATTGACAAAGTACC+AGG | 0.582389 | 1:+81884507 | None:intergenic |
| GGTGAATGGATTTGCATGCA+AGG | 0.593738 | 1:-81884910 | MsG0180004728.01.T01:CDS |
| CTGTTTAGTATCAATCTGAA+AGG | 0.609012 | 1:+81884434 | None:intergenic |
| TCTGTTTGACTCTACACCAA+CGG | 0.619003 | 1:-81884481 | MsG0180004728.01.T01:CDS |
| GTGGCACGAGGGTGTGTGTG+TGG | 0.621879 | 1:+81884711 | None:intergenic |
| AGAACATCATCAGGAACCGT+TGG | 0.626429 | 1:+81884465 | None:intergenic |
| AAGGTTGTCAACAACACTGT+AGG | 0.630939 | 1:-81884834 | MsG0180004728.01.T01:CDS |
| AAAACAACCTCAGTGGCACG+AGG | 0.637231 | 1:+81884699 | None:intergenic |
| CACACACCCTCGTGCCACTG+AGG | 0.678341 | 1:-81884706 | MsG0180004728.01.T01:CDS |
| AAACAACCTCAGTGGCACGA+GGG | 0.703640 | 1:+81884700 | None:intergenic |
| GCATTATCAGAAGAATAATG+GGG | 0.712368 | 1:-81884571 | MsG0180004728.01.T01:CDS |
| ATGATGGAAGAGCTACACAA+AGG | 0.722041 | 1:+81885531 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATATAAAAATAGTTGAAC+TGG | - | Chr1:81884968-81884987 | MsG0180004728.01.T01:intron | 15.0% |
| !!! | TACTACTATTATCATTTTTT+TGG | - | Chr1:81884561-81884580 | MsG0180004728.01.T01:CDS | 15.0% |
| !! | AAAAAAATTGTCCAATTTGT+TGG | + | Chr1:81884401-81884420 | None:intergenic | 20.0% |
| !! | ATTTCTAAGTCCATAAAAAA+AGG | - | Chr1:81885385-81885404 | MsG0180004728.01.T01:intron | 20.0% |
| !! | CAAAGTTATGTCTAATATTA+TGG | - | Chr1:81885055-81885074 | MsG0180004728.01.T01:intron | 20.0% |
| !!! | AAAGATTTCACCTTTTTTTA+TGG | + | Chr1:81885398-81885417 | None:intergenic | 20.0% |
| ! | ATTTGAAGGAGTTAAAGAAT+CGG | + | Chr1:81884860-81884879 | None:intergenic | 25.0% |
| ! | GATTAAATTGTTTATGCAAC+TGG | - | Chr1:81885011-81885030 | MsG0180004728.01.T01:intron | 25.0% |
| ! | TATTATGGTATGTCTTATGT+AGG | - | Chr1:81885070-81885089 | MsG0180004728.01.T01:intron | 25.0% |
| ! | TGCATTATCAGAAGAATAAT+GGG | - | Chr1:81885437-81885456 | MsG0180004728.01.T01:intron | 25.0% |
| ! | TGTAACTATTGCTAAAATTG+TGG | - | Chr1:81884668-81884687 | MsG0180004728.01.T01:CDS | 25.0% |
| ! | TGTATAATGCTTCATGTATA+TGG | - | Chr1:81884508-81884527 | MsG0180004728.01.T01:CDS | 25.0% |
| !!! | AAGTTGTTTTCATAATCACA+CGG | + | Chr1:81884950-81884969 | None:intergenic | 25.0% |
| AAATCTTTGTGTTCCCAAAA+GGG | - | Chr1:81885410-81885429 | MsG0180004728.01.T01:intron | 30.0% | |
| AACTATTGCTAAAATTGTGG+AGG | - | Chr1:81884671-81884690 | MsG0180004728.01.T01:CDS | 30.0% | |
| AGAATAGACTACAAACCTAA+TGG | - | Chr1:81885265-81885284 | MsG0180004728.01.T01:intron | 30.0% | |
| CATTATACATTACCTGATGA+TGG | + | Chr1:81884497-81884516 | None:intergenic | 30.0% | |
| CTCTAACTTAGTGTTATGTT+TGG | - | Chr1:81884587-81884606 | MsG0180004728.01.T01:CDS | 30.0% | |
| CTGTTTAGTATCAATCTGAA+AGG | + | Chr1:81885578-81885597 | None:intergenic | 30.0% | |
| CTTTCAGATTGATACTAAAC+AGG | - | Chr1:81885576-81885595 | MsG0180004728.01.T01:CDS | 30.0% | |
| GCATTATCAGAAGAATAATG+GGG | - | Chr1:81885438-81885457 | MsG0180004728.01.T01:intron | 30.0% | |
| GTGCATTATCAGAAGAATAA+TGG | - | Chr1:81885436-81885455 | MsG0180004728.01.T01:intron | 30.0% | |
| TATGTCTTATGTAGGTGTTA+AGG | - | Chr1:81885078-81885097 | MsG0180004728.01.T01:intron | 30.0% | |
| ! | CAATGAGTTTTGCAAAATCA+AGG | - | Chr1:81884615-81884634 | MsG0180004728.01.T01:CDS | 30.0% |
| !! | AGTTGTTTTCATAATCACAC+GGG | + | Chr1:81884949-81884968 | None:intergenic | 30.0% |
| !!! | TCCAGGAATTTTTTCAACAT+TGG | + | Chr1:81885218-81885237 | None:intergenic | 30.0% |
| ATTCCCTTATGTACTCTTGA+GGG | - | Chr1:81884749-81884768 | MsG0180004728.01.T01:CDS | 35.0% | |
| GAAATCTTTGTGTTCCCAAA+AGG | - | Chr1:81885409-81885428 | MsG0180004728.01.T01:intron | 35.0% | |
| GCCAATGTTGAAAAAATTCC+TGG | - | Chr1:81885214-81885233 | MsG0180004728.01.T01:intron | 35.0% | |
| TATAGAGTGCATAGTTCACT+CGG | + | Chr1:81884896-81884915 | None:intergenic | 35.0% | |
| TATGTAGGTGTTAAGGTGAA+TGG | - | Chr1:81885085-81885104 | MsG0180004728.01.T01:intron | 35.0% | |
| TTGGAAGGTGAATTAGATGT+AGG | - | Chr1:81885337-81885356 | MsG0180004728.01.T01:intron | 35.0% | |
| ! | ATGAGAGTGCAAAAAACTCA+AGG | + | Chr1:81884718-81884737 | None:intergenic | 35.0% |
| ! | GAAACAATTGACAAAGTACC+AGG | + | Chr1:81885505-81885524 | None:intergenic | 35.0% |
| !!! | GCAGCTGATTTTTTCTTTGA+TGG | - | Chr1:81885139-81885158 | MsG0180004728.01.T01:intron | 35.0% |
| AAGGTTGTCAACAACACTGT+AGG | - | Chr1:81885175-81885194 | MsG0180004728.01.T01:intron | 40.0% | |
| AATTCCTGGACTCAACACAT+TGG | - | Chr1:81885228-81885247 | MsG0180004728.01.T01:intron | 40.0% | |
| ATTCCTGGACTCAACACATT+GGG | - | Chr1:81885229-81885248 | MsG0180004728.01.T01:intron | 40.0% | |
| CAAAGGTCTTGAAGTGTATC+AGG | + | Chr1:81884464-81884483 | None:intergenic | 40.0% | |
| CAACAACACTGTAGGATCTT+TGG | - | Chr1:81885183-81885202 | MsG0180004728.01.T01:intron | 40.0% | |
| CAACACAAAAACAACCTCAG+TGG | + | Chr1:81885320-81885339 | None:intergenic | 40.0% | |
| GATTCCCTTATGTACTCTTG+AGG | - | Chr1:81884748-81884767 | MsG0180004728.01.T01:CDS | 40.0% | |
| GTTGAGCTAAGGGAATTTGA+AGG | + | Chr1:81884874-81884893 | None:intergenic | 40.0% | |
| TCTGTTTGACTCTACACCAA+CGG | - | Chr1:81885528-81885547 | MsG0180004728.01.T01:CDS | 40.0% | |
| TGATGGTTTAGCAAACCCTA+AGG | - | Chr1:81885156-81885175 | MsG0180004728.01.T01:intron | 40.0% | |
| ! | ACAGTGTTGTTGACAACCTT+AGG | + | Chr1:81885175-81885194 | None:intergenic | 40.0% |
| ! | AGACCCTCAAGAGTACATAA+GGG | + | Chr1:81884755-81884774 | None:intergenic | 40.0% |
| ! | ATGATGGAAGAGCTACACAA+AGG | + | Chr1:81884481-81884500 | None:intergenic | 40.0% |
| ! | CAGTGTTGTTGACAACCTTA+GGG | + | Chr1:81885174-81885193 | None:intergenic | 40.0% |
| ! | GATAATGCACCAACCCTTTT+GGG | + | Chr1:81885426-81885445 | None:intergenic | 40.0% |
| ! | GCTTTTAACAGTCAACTTCC+TGG | - | Chr1:81885484-81885503 | MsG0180004728.01.T01:intron | 40.0% |
| !! | TGATAATGCACCAACCCTTT+TGG | + | Chr1:81885427-81885446 | None:intergenic | 40.0% |
| !!! | CACTGAGGTTGTTTTTGTGT+TGG | - | Chr1:81885318-81885337 | MsG0180004728.01.T01:intron | 40.0% |
| !!! | GAGGTTGTTTTTGTGTTGGA+AGG | - | Chr1:81885322-81885341 | MsG0180004728.01.T01:intron | 40.0% |
| AGAACATCATCAGGAACCGT+TGG | + | Chr1:81885547-81885566 | None:intergenic | 45.0% | |
| AGAAGCGAAGGTAGATGAGA+TGG | + | Chr1:81884438-81884457 | None:intergenic | 45.0% | |
| AGTTCACTCGGTTGAGCTAA+GGG | + | Chr1:81884884-81884903 | None:intergenic | 45.0% | |
| CTTTGTGTTCCCAAAAGGGT+TGG | - | Chr1:81885414-81885433 | MsG0180004728.01.T01:intron | 45.0% | |
| TAGTTCACTCGGTTGAGCTA+AGG | + | Chr1:81884885-81884904 | None:intergenic | 45.0% | |
| TCTTGAGGGTCTGTTTGGAT+TGG | - | Chr1:81884763-81884782 | MsG0180004728.01.T01:CDS | 45.0% | |
| TGTACTCTTGAGGGTCTGTT+TGG | - | Chr1:81884758-81884777 | MsG0180004728.01.T01:CDS | 45.0% | |
| TGTATCAGGATCAGAAGCGA+AGG | + | Chr1:81884450-81884469 | None:intergenic | 45.0% | |
| TGTGTAGCTCTTCCATCATC+AGG | - | Chr1:81884482-81884501 | MsG0180004728.01.T01:CDS | 45.0% | |
| ! | CAGACCCTCAAGAGTACATA+AGG | + | Chr1:81884756-81884775 | None:intergenic | 45.0% |
| ! | GGTGAATGGATTTGCATGCA+AGG | - | Chr1:81885099-81885118 | MsG0180004728.01.T01:intron | 45.0% |
| ! | TCCTGATGATGTTCTTGCTC+AGG | - | Chr1:81885552-81885571 | MsG0180004728.01.T01:CDS | 45.0% |
| AAAACAACCTCAGTGGCACG+AGG | + | Chr1:81885313-81885332 | None:intergenic | 50.0% | |
| AAACAACCTCAGTGGCACGA+GGG | + | Chr1:81885312-81885331 | None:intergenic | 50.0% | |
| ACTCCCAATGTGTTGAGTCC+AGG | + | Chr1:81885235-81885254 | None:intergenic | 50.0% | |
| GCCTGAGCAAGAACATCATC+AGG | + | Chr1:81885556-81885575 | None:intergenic | 50.0% | |
| !! | TGTGGCGGGTTAAGACCATT+AGG | + | Chr1:81885283-81885302 | None:intergenic | 50.0% |
| ! | CACACACCCTCGTGCCACTG+AGG | - | Chr1:81885303-81885322 | MsG0180004728.01.T01:intron | 65.0% |
| ! | CACGAGGGTGTGTGTGTGGC+GGG | + | Chr1:81885297-81885316 | None:intergenic | 65.0% |
| ! | GCACGAGGGTGTGTGTGTGG+CGG | + | Chr1:81885298-81885317 | None:intergenic | 65.0% |
| ! | GTGGCACGAGGGTGTGTGTG+TGG | + | Chr1:81885301-81885320 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 81884392 | 81885639 | 81884392 | ID=MsG0180004728.01;Name=MsG0180004728.01 |
| Chr1 | mRNA | 81884392 | 81885639 | 81884392 | ID=MsG0180004728.01.T01;Parent=MsG0180004728.01;Name=MsG0180004728.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|218 |
| Chr1 | exon | 81885523 | 81885639 | 81885523 | ID=MsG0180004728.01.T01:exon:35068;Parent=MsG0180004728.01.T01 |
| Chr1 | exon | 81884392 | 81884931 | 81884392 | ID=MsG0180004728.01.T01:exon:35067;Parent=MsG0180004728.01.T01 |
| Chr1 | CDS | 81885523 | 81885639 | 81885523 | ID=MsG0180004728.01.T01:cds;Parent=MsG0180004728.01.T01 |
| Chr1 | CDS | 81884392 | 81884931 | 81884392 | ID=MsG0180004728.01.T01:cds;Parent=MsG0180004728.01.T01 |
| Gene Sequence |
| Protein sequence |
