Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004987.01.T01 | PKU82897.1 | 68.571 | 105 | 17 | 1 | 3 | 107 | 24 | 112 | 4.13E-41 | 146 |
| MsG0180004987.01.T02 | PKU82897.1 | 68.571 | 105 | 17 | 1 | 3 | 107 | 24 | 112 | 4.13E-41 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004987.01.T01 | A0A1D1YKD6 | 56.757 | 185 | 28 | 4 | 3 | 163 | 179 | 335 | 3.20e-54 | 184 |
| MsG0180004987.01.T02 | A0A1D1YKD6 | 56.757 | 185 | 28 | 4 | 3 | 163 | 179 | 335 | 3.20e-54 | 184 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0180004987.01 | 0.861209 | 1.205177e-63 | 3.383352e-60 |
| MsG0080047945.01 | MsG0180004987.01 | 0.823198 | 1.575703e-53 | 1.347572e-50 |
| MsG0080047955.01 | MsG0180004987.01 | 0.840888 | 6.628756e-58 | 9.575416e-55 |
| MsG0080047956.01 | MsG0180004987.01 | 0.827045 | 1.943340e-54 | 1.854580e-51 |
| MsG0080047989.01 | MsG0180004987.01 | 0.841272 | 5.254943e-58 | 7.680915e-55 |
| MsG0080048068.01 | MsG0180004987.01 | 0.885627 | 6.909049e-72 | 4.777170e-68 |
| MsG0080048191.01 | MsG0180004987.01 | 0.804120 | 2.515961e-49 | 1.286702e-46 |
| MsG0080048198.01 | MsG0180004987.01 | 0.824508 | 7.770845e-54 | 6.896992e-51 |
| MsG0080048273.01 | MsG0180004987.01 | 0.834764 | 2.481439e-56 | 2.973333e-53 |
| MsG0080048505.01 | MsG0180004987.01 | 0.839500 | 1.526901e-57 | 2.112662e-54 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004987.01.T01 | MTR_1g090730 | 60.870 | 138 | 18 | 3 | 50 | 163 | 312 | 437 | 9.85e-41 | 142 |
| MsG0180004987.01.T01 | MTR_1g090730 | 94.340 | 53 | 3 | 0 | 3 | 55 | 168 | 220 | 1.13e-26 | 104 |
| MsG0180004987.01.T01 | MTR_1g090730 | 69.565 | 92 | 7 | 1 | 50 | 120 | 312 | 403 | 9.05e-33 | 120 |
| MsG0180004987.01.T01 | MTR_1g090730 | 92.593 | 54 | 4 | 0 | 3 | 56 | 168 | 221 | 5.15e-27 | 105 |
| MsG0180004987.01.T02 | MTR_1g090730 | 60.870 | 138 | 18 | 3 | 50 | 163 | 312 | 437 | 9.85e-41 | 142 |
| MsG0180004987.01.T02 | MTR_1g090730 | 94.340 | 53 | 3 | 0 | 3 | 55 | 168 | 220 | 1.13e-26 | 104 |
| MsG0180004987.01.T02 | MTR_1g090730 | 69.565 | 92 | 7 | 1 | 50 | 120 | 312 | 403 | 9.05e-33 | 120 |
| MsG0180004987.01.T02 | MTR_1g090730 | 92.593 | 54 | 4 | 0 | 3 | 56 | 168 | 221 | 5.15e-27 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004987.01.T01 | AT5G58330 | 55.725 | 131 | 29 | 6 | 54 | 163 | 212 | 334 | 8.01e-34 | 122 |
| MsG0180004987.01.T01 | AT5G58330 | 84.906 | 53 | 8 | 0 | 3 | 55 | 64 | 116 | 1.31e-23 | 94.7 |
| MsG0180004987.01.T01 | AT5G58330 | 55.725 | 131 | 29 | 6 | 54 | 163 | 320 | 442 | 4.16e-33 | 122 |
| MsG0180004987.01.T01 | AT5G58330 | 84.906 | 53 | 8 | 0 | 3 | 55 | 172 | 224 | 2.04e-23 | 95.5 |
| MsG0180004987.01.T01 | AT5G58330 | 55.725 | 131 | 29 | 6 | 54 | 163 | 321 | 443 | 4.21e-33 | 122 |
| MsG0180004987.01.T01 | AT5G58330 | 84.906 | 53 | 8 | 0 | 3 | 55 | 173 | 225 | 2.07e-23 | 95.5 |
| MsG0180004987.01.T02 | AT5G58330 | 55.725 | 131 | 29 | 6 | 54 | 163 | 212 | 334 | 8.01e-34 | 122 |
| MsG0180004987.01.T02 | AT5G58330 | 84.906 | 53 | 8 | 0 | 3 | 55 | 64 | 116 | 1.31e-23 | 94.7 |
| MsG0180004987.01.T02 | AT5G58330 | 55.725 | 131 | 29 | 6 | 54 | 163 | 320 | 442 | 4.16e-33 | 122 |
| MsG0180004987.01.T02 | AT5G58330 | 84.906 | 53 | 8 | 0 | 3 | 55 | 172 | 224 | 2.04e-23 | 95.5 |
Find 35 sgRNAs with CRISPR-Local
Find 488 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACACACACATGGATAACTT+TGG | 0.419395 | 1:+85265419 | None:intergenic |
| AACCATTATTCTACATCAAT+TGG | 0.372547 | 1:+85265627 | None:intergenic |
| AATAATGGTTATGTAATAAA+AGG | 0.243255 | 1:-85265614 | MsG0180004987.01.T01:three_prime_UTR |
| AATTATTGTGTTCTACAAGC+TGG | 0.418187 | 1:-85265115 | MsG0180004987.01.T01:intron |
| ACACACACATGGATAACTTT+GGG | 0.349611 | 1:+85265420 | None:intergenic |
| ACACACATGGATAACTTTGG+GGG | 0.678822 | 1:+85265422 | None:intergenic |
| ACATTTAGAAAGTTTCAAGT+TGG | 0.545610 | 1:+85265332 | None:intergenic |
| ACTTGAAACTTTCTAAATGT+AGG | 0.440684 | 1:-85265329 | MsG0180004987.01.T01:three_prime_UTR |
| AGAGTTACCTCAAAATATGA+CGG | 0.635658 | 1:+85265466 | None:intergenic |
| AGCTCGTCAACATGTATAAG+TGG | 0.470207 | 1:-85265188 | MsG0180004987.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAACATTTGTTTGAAGGC+AGG | + | Chr1:85269722-85269741 | None:intergenic | 30.0% | |
| AATACCGTGGAAAATTTGAA+AGG | + | Chr1:85264046-85264065 | None:intergenic | 30.0% | |
| AATGTCAAAGTTATAGTCGT+GGG | - | Chr1:85264809-85264828 | MsG0180004987.01.T01:intron | 30.0% | |
| AATTAATACCATTGCAGTCT+CGG | - | Chr1:85264148-85264167 | MsG0180004987.01.T01:intron | 30.0% | |
| AATTATTGTGTTCTACAAGC+TGG | - | Chr1:85266878-85266897 | MsG0180004987.01.T01:intron | 30.0% | |
| ACCACATAAATGTGATCTAA+TGG | - | Chr1:85268676-85268695 | MsG0180004987.01.T01:intron | 30.0% | |
| ACCATTAGATCACATTTATG+TGG | + | Chr1:85268680-85268699 | None:intergenic | 30.0% | |
| AGAGTTACCTCAAAATATGA+CGG | + | Chr1:85266530-85266549 | None:intergenic | 30.0% | |
| ATAGAATAGACTGTGATGAA+AGG | + | Chr1:85267741-85267760 | None:intergenic | 30.0% | |
| ATATCTTCAGCTATTCCATA+AGG | + | Chr1:85265748-85265767 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 85262197 | 85269818 | 85262197 | ID=MsG0180004987.01;Name=MsG0180004987.01 |
| Chr1 | mRNA | 85262197 | 85269818 | 85262197 | ID=MsG0180004987.01.T01;Parent=MsG0180004987.01;Name=MsG0180004987.01.T01;_AED=0.24;_eAED=0.24;_QI=651|0.66|0.7|1|0.11|0.1|10|572|163 |
| Chr1 | exon | 85262197 | 85262234 | 85262197 | ID=MsG0180004987.01.T01:exon:28114;Parent=MsG0180004987.01.T01 |
| Chr1 | exon | 85265165 | 85265752 | 85265165 | ID=MsG0180004987.01.T01:exon:28113;Parent=MsG0180004987.01.T01 |
| Chr1 | five_prime_UTR | 85269497 | 85269818 | 85269497 | ID=MsG0180004987.01.T01:five_prime_utr;Parent=MsG0180004987.01.T01 |
| Chr1 | five_prime_UTR | 85269321 | 85269411 | 85269321 | ID=MsG0180004987.01.T01:five_prime_utr;Parent=MsG0180004987.01.T01 |
| Chr1 | five_prime_UTR | 85267807 | 85267892 | 85267807 | ID=MsG0180004987.01.T01:five_prime_utr;Parent=MsG0180004987.01.T01 |
| Chr1 | five_prime_UTR | 85267623 | 85267706 | 85267623 | ID=MsG0180004987.01.T01:five_prime_utr;Parent=MsG0180004987.01.T01 |
| Chr1 | five_prime_UTR | 85267437 | 85267504 | 85267437 | ID=MsG0180004987.01.T01:five_prime_utr;Parent=MsG0180004987.01.T01 |
| Chr1 | CDS | 85267326 | 85267436 | 85267326 | ID=MsG0180004987.01.T01:cds;Parent=MsG0180004987.01.T01 |
| Chr1 | CDS | 85267189 | 85267236 | 85267189 | ID=MsG0180004987.01.T01:cds;Parent=MsG0180004987.01.T01 |
| Chr1 | CDS | 85266427 | 85266627 | 85266427 | ID=MsG0180004987.01.T01:cds;Parent=MsG0180004987.01.T01 |
| Chr1 | CDS | 85265834 | 85265911 | 85265834 | ID=MsG0180004987.01.T01:cds;Parent=MsG0180004987.01.T01 |
| Chr1 | CDS | 85265699 | 85265752 | 85265699 | ID=MsG0180004987.01.T01:cds;Parent=MsG0180004987.01.T01 |
| Chr1 | three_prime_UTR | 85265165 | 85265698 | 85265165 | ID=MsG0180004987.01.T01:three_prime_utr;Parent=MsG0180004987.01.T01 |
| Chr1 | three_prime_UTR | 85262197 | 85262234 | 85262197 | ID=MsG0180004987.01.T01:three_prime_utr;Parent=MsG0180004987.01.T01 |
| Chr1 | mRNA | 85265098 | 85269818 | 85265098 | ID=MsG0180004987.01.T02;Parent=MsG0180004987.01;Name=MsG0180004987.01.T02;_AED=0.24;_eAED=0.24;_QI=651|0.62|0.66|1|0.12|0.22|9|601|163 |
| Chr1 | exon | 85265098 | 85265752 | 85265098 | ID=MsG0180004987.01.T02:exon:28115;Parent=MsG0180004987.01.T02 |
| Chr1 | five_prime_UTR | 85269497 | 85269818 | 85269497 | ID=MsG0180004987.01.T02:five_prime_utr;Parent=MsG0180004987.01.T02 |
| Chr1 | five_prime_UTR | 85269321 | 85269411 | 85269321 | ID=MsG0180004987.01.T02:five_prime_utr;Parent=MsG0180004987.01.T02 |
| Chr1 | five_prime_UTR | 85267807 | 85267892 | 85267807 | ID=MsG0180004987.01.T02:five_prime_utr;Parent=MsG0180004987.01.T02 |
| Chr1 | five_prime_UTR | 85267623 | 85267706 | 85267623 | ID=MsG0180004987.01.T02:five_prime_utr;Parent=MsG0180004987.01.T02 |
| Chr1 | five_prime_UTR | 85267437 | 85267504 | 85267437 | ID=MsG0180004987.01.T02:five_prime_utr;Parent=MsG0180004987.01.T02 |
| Chr1 | CDS | 85267326 | 85267436 | 85267326 | ID=MsG0180004987.01.T02:cds;Parent=MsG0180004987.01.T02 |
| Chr1 | CDS | 85267189 | 85267236 | 85267189 | ID=MsG0180004987.01.T02:cds;Parent=MsG0180004987.01.T02 |
| Chr1 | CDS | 85266427 | 85266627 | 85266427 | ID=MsG0180004987.01.T02:cds;Parent=MsG0180004987.01.T02 |
| Chr1 | CDS | 85265834 | 85265911 | 85265834 | ID=MsG0180004987.01.T02:cds;Parent=MsG0180004987.01.T02 |
| Chr1 | CDS | 85265699 | 85265752 | 85265699 | ID=MsG0180004987.01.T02:cds;Parent=MsG0180004987.01.T02 |
| Chr1 | three_prime_UTR | 85265098 | 85265698 | 85265098 | ID=MsG0180004987.01.T02:three_prime_utr;Parent=MsG0180004987.01.T02 |
| Gene Sequence |
>MsG0180004987.01.T02 ATGAGGTTCCAAGATGCAGAGTGGGCTTTGCTAATAGGTGCAAAGCCTCGAGGACCTGGAATGGAGCGTGCGGCTTTGCTAGACATAAATGGGCAGATTTTTGCGGAGCAGGGAAAAGCACTCAATGCGGTTGCATCTCGCAATGTCAAAGTTATAGTCAGAGGTGGTGCACTTATTCAAAAATGGGGAAGATCATCTGCTGCATCGACTTCTGTGTCTATAGTTGATGCCATACGATCTTTGATCACTCCTACTCCGGAGGGTGATTGGTTTTCTACTGGTGTAAGTTTGTATTTCTTAAACAAACTTGTGTATTTACTTAATATATTCACCAATGTCGAGACCCATGCGCATTCACGTACAGAAGCTGAGTTATTGGCGGAGAAAAAATGTGTGGCTCACCTAACCGGCGAGGTAAATATTGCGGCCCGAATCGCGGGGATTGCTGTTTGTGATCTACCTGGTGATACCATGCTCCCAGGAGAAATGTAA >MsG0180004987.01.T01 ATGAGGTTCCAAGATGCAGAGTGGGCTTTGCTAATAGGTGCAAAGCCTCGAGGACCTGGAATGGAGCGTGCGGCTTTGCTAGACATAAATGGGCAGATTTTTGCGGAGCAGGGAAAAGCACTCAATGCGGTTGCATCTCGCAATGTCAAAGTTATAGTCAGAGGTGGTGCACTTATTCAAAAATGGGGAAGATCATCTGCTGCATCGACTTCTGTGTCTATAGTTGATGCCATACGATCTTTGATCACTCCTACTCCGGAGGGTGATTGGTTTTCTACTGGTGTAAGTTTGTATTTCTTAAACAAACTTGTGTATTTACTTAATATATTCACCAATGTCGAGACCCATGCGCATTCACGTACAGAAGCTGAGTTATTGGCGGAGAAAAAATGTGTGGCTCACCTAACCGGCGAGGTAAATATTGCGGCCCGAATCGCGGGGATTGCTGTTTGTGATCTACCTGGTGATACCATGCTCCCAGGAGAAATGTAA |
| Protein sequence |
>MsG0180004987.01.T02 MRFQDAEWALLIGAKPRGPGMERAALLDINGQIFAEQGKALNAVASRNVKVIVRGGALIQKWGRSSAASTSVSIVDAIRSLITPTPEGDWFSTGVSLYFLNKLVYLLNIFTNVETHAHSRTEAELLAEKKCVAHLTGEVNIAARIAGIAVCDLPGDTMLPGEM* >MsG0180004987.01.T01 MRFQDAEWALLIGAKPRGPGMERAALLDINGQIFAEQGKALNAVASRNVKVIVRGGALIQKWGRSSAASTSVSIVDAIRSLITPTPEGDWFSTGVSLYFLNKLVYLLNIFTNVETHAHSRTEAELLAEKKCVAHLTGEVNIAARIAGIAVCDLPGDTMLPGEM* |
