AlfalfaGEDB | MsG0180005730.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005730.01

KEGG: K00913
GO: GO:0000287 GO:0005524 GO:0005622 GO:0032957 GO:0047325 GO:0052725 GO:0052726
PFAM: PF05770

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005730.01.T01	MCI12621.1	74.468	47	12	0	282	328	6	52	4.29E-15	79.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005730.01.T01	O81893	81.081	37	7	0	283	319	107	143	9.64E-13	72

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005730.01.T01	A0A072VQB8	100.000	38	0	0	282	319	56	93	2.30e-14	84.0

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047797.01	MsG0180005730.01	0.809646	1.707534e-50	1.008144e-47
MsG0080047957.01	MsG0180005730.01	0.828138	1.062244e-54	1.046661e-51
MsG0080047958.01	MsG0180005730.01	0.810998	8.719535e-51	5.335924e-48
MsG0080047959.01	MsG0180005730.01	0.804922	1.711669e-49	8.937147e-47
MsG0080048463.01	MsG0180005730.01	0.805899	1.068314e-49	5.719368e-47
MsG0080048464.01	MsG0180005730.01	0.835724	1.420278e-56	1.751789e-53
MsG0080048672.01	MsG0180005730.01	0.825020	5.884804e-54	5.300842e-51
MsG0080048683.01	MsG0180005730.01	0.837150	6.160297e-57	7.931493e-54
MsG0080048787.01	MsG0180005730.01	0.803942	2.739404e-49	1.394666e-46
MsG0080049002.01	MsG0180005730.01	0.807971	3.895192e-50	2.199894e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005730.01.T01	MTR_1g105930	100.000	38	0	0	282	319	56	93	5.84e-18	84.0
MsG0180005730.01.T01	MTR_1g105930	65.909	88	23	3	157	244	1	81	1.13e-14	74.3
MsG0180005730.01.T01	MTR_5g005370	78.947	38	8	0	282	319	2	39	2.74e-13	69.7
MsG0180005730.01.T01	MTR_5g005370	78.947	38	8	0	282	319	9	46	2.85e-13	69.7
MsG0180005730.01.T01	MTR_5g005370	78.947	38	8	0	282	319	37	74	2.89e-13	70.1
MsG0180005730.01.T01	MTR_5g005370	78.947	38	8	0	282	319	37	74	3.44e-13	70.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005730.01.T01	AT4G33770	64.151	53	11	1	267	319	6	50	5.12e-14	72.0
MsG0180005730.01.T01	AT4G33770	81.081	37	7	0	283	319	107	143	9.83e-14	72.0
MsG0180005730.01.T01	AT4G08170	71.429	42	12	0	282	323	66	107	2.88e-12	67.4

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Find 54 sgRNAs with CRISPR-Local

Find 579 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGCCACTGTCAGAACA+AGG	0.605835	1:-95380788	MsG0180005730.01.T01:CDS
AAGTTGCGTGTTCTGACAAT+GGG	0.504150	1:-95389309	MsG0180005730.01.T01:CDS
AATGGACCTTGTTCTGACAG+TGG	0.636759	1:+95380782	None:intergenic
AATTATGCGTTGTCAGTTGG+GGG	0.627727	1:-95383808	MsG0180005730.01.T01:CDS
AATTGAGAAAGAGGAGAACC+TGG	0.546028	1:-95389425	MsG0180005730.01.T01:CDS
ACGGAGTTCGATGGAAATCA+CGG	0.610047	1:-95383322	MsG0180005730.01.T01:CDS
ACTGACAACGCATAATTAAG+TGG	0.528911	1:+95383814	None:intergenic
AGAAGTGATGGCGAAATCTG+AGG	0.629932	1:-95382217	MsG0180005730.01.T01:CDS
AGATTAGATTTATTTAGTTT+GGG	0.362125	1:-95389255	MsG0180005730.01.T01:CDS
AGGTAAAGGCAATGACCATT+TGG	0.447715	1:-95383782	MsG0180005730.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATGCTAGCTACTTACAA+AGG	+	Chr1:95384725-95384744	None:intergenic	30.0%
AAAGAAAAAAGGAAGGAGAA+GGG	-	Chr1:95383058-95383077	MsG0180005730.01.T01:intron	30.0%
AAATTATTAACACTCAGCAG+TGG	-	Chr1:95388915-95388934	MsG0180005730.01.T01:intron	30.0%
AACACGTAGGAAATAAAAAC+AGG	+	Chr1:95382639-95382658	None:intergenic	30.0%
AACATTATTAGCTAGTACCT+CGG	+	Chr1:95385624-95385643	None:intergenic	30.0%
AATGAGGAAAGAAAAAAGGA+AGG	-	Chr1:95383051-95383070	MsG0180005730.01.T01:intron	30.0%
ACACGTAGGAAATAAAAACA+GGG	+	Chr1:95382638-95382657	None:intergenic	30.0%
AGATTTGATCTTTGAATCAG+GGG	-	Chr1:95387646-95387665	MsG0180005730.01.T01:intron	30.0%
ATATATGTTTGAAGGATGTG+TGG	+	Chr1:95384166-95384185	None:intergenic	30.0%
ATGAATTATAGCTGAGAATC+GGG	+	Chr1:95385067-95385086	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	95380472	95389487	95380472	ID=MsG0180005730.01;Name=MsG0180005730.01
Chr1	mRNA	95380472	95389487	95380472	ID=MsG0180005730.01.T01;Parent=MsG0180005730.01;Name=MsG0180005730.01.T01;_AED=0.70;_eAED=0.84;_QI=0\|0.16\|0.14\|0.57\|0.66\|0.71\|7\|0\|334
Chr1	exon	95389246	95389487	95389246	ID=MsG0180005730.01.T01:exon:10464;Parent=MsG0180005730.01.T01
Chr1	exon	95383753	95383840	95383753	ID=MsG0180005730.01.T01:exon:10463;Parent=MsG0180005730.01.T01
Chr1	exon	95383301	95383425	95383301	ID=MsG0180005730.01.T01:exon:10462;Parent=MsG0180005730.01.T01
Chr1	exon	95382066	95382293	95382066	ID=MsG0180005730.01.T01:exon:10461;Parent=MsG0180005730.01.T01
Chr1	exon	95381700	95381858	95381700	ID=MsG0180005730.01.T01:exon:10460;Parent=MsG0180005730.01.T01
Chr1	exon	95380763	95380844	95380763	ID=MsG0180005730.01.T01:exon:10459;Parent=MsG0180005730.01.T01
Chr1	exon	95380472	95380552	95380472	ID=MsG0180005730.01.T01:exon:10458;Parent=MsG0180005730.01.T01
Chr1	CDS	95389246	95389487	95389246	ID=MsG0180005730.01.T01:cds;Parent=MsG0180005730.01.T01
Chr1	CDS	95383753	95383840	95383753	ID=MsG0180005730.01.T01:cds;Parent=MsG0180005730.01.T01
Chr1	CDS	95383301	95383425	95383301	ID=MsG0180005730.01.T01:cds;Parent=MsG0180005730.01.T01
Chr1	CDS	95382066	95382293	95382066	ID=MsG0180005730.01.T01:cds;Parent=MsG0180005730.01.T01
Chr1	CDS	95381700	95381858	95381700	ID=MsG0180005730.01.T01:cds;Parent=MsG0180005730.01.T01
Chr1	CDS	95380763	95380844	95380763	ID=MsG0180005730.01.T01:cds;Parent=MsG0180005730.01.T01
Chr1	CDS	95380472	95380552	95380472	ID=MsG0180005730.01.T01:cds;Parent=MsG0180005730.01.T01

Gene Sequence

>MsG0180005730.01.T01

ATGTTGTTAATCAAATCGCGTTGGAAAATCGGAAAAGTGAAATTGAGAAAGAGGAGAACCTGGATGAGTTTCGAACTGAATCTGGCGGCGTCGGCGTTTTCCGGTCAGATTTTGGTTTTGACGGCGGAGATGAGGTCAGCACTATGGACTGAGTACAAGTTGCGTGTTCTGACAATGGGAAAGATAATGACGCTACTGAATTTTAGTGTTAGATTAGATTTATTTAGTTTGGGAGTTTTAGGTTGCCCACTTAATTATGCGTTGTCAGTTGGGGGCAAAGGTAAAGGCAATGACCATTTGGACATGACATTTACTGTGAAGCTTCATCATTTTGCAGCATCTATGAAGACGGTTAGATCTGGCAAGAAATTTGTATCTCTCTATGTGTCTTGTTGGTACAATGTTTGTAAAACGGAGTTCGATGGAAATCACGGTAGAATTGCACTAGATAACCTGGGTATTTTCGGTATGAGACTAAACGGAGAAATCTCAAGCGAAGAAGAAGAAGAAGAAGTGATGGCGAAATCTGAGGTGAGTACGACACCGTTTTTGGGTCAGAAAATTGTTGTTGGGTATGCTTTAACTGCGAAGAAGAAGAAAAGTTTTCTTCAGCCAAATTTCATTTCTCTTGCAAGGTACAATTTTTACTATTTACCAATGATTTTCTTTTTGATCATTTTCATTGCGAGTAAAAGTCTCACTTTTGATGAAAAAGCTGACATAGAACTACATATAAGTGAGATGACTGATATATCCGATGTAATGTCTGTAGCTCAATGTGATGATCCCCGAGCTCCATTCGTGACCGACACTTTTAGATTTCTTGTAAACATACCAAACTGGAGCAAGGGGATATACTTTGTTAACATTGATCTAAACAAGCCACTGTCAGAACAAGGTCCATTTGACATTGTCTTGCATAAGTTGTTAGGCGAGGAATGGCGAGAGATCATTGAGGTTTGTAAACTCCACTCACTTCAGCCTACTTTCACATTGTACTTGTAA

Protein sequence

>MsG0180005730.01.T01

MLLIKSRWKIGKVKLRKRRTWMSFELNLAASAFSGQILVLTAEMRSALWTEYKLRVLTMGKIMTLLNFSVRLDLFSLGVLGCPLNYALSVGGKGKGNDHLDMTFTVKLHHFAASMKTVRSGKKFVSLYVSCWYNVCKTEFDGNHGRIALDNLGIFGMRLNGEISSEEEEEEVMAKSEVSTTPFLGQKIVVGYALTAKKKKSFLQPNFISLARYNFYYLPMIFFLIIFIASKSLTFDEKADIELHISEMTDISDVMSVAQCDDPRAPFVTDTFRFLVNIPNWSKGIYFVNIDLNKPLSEQGPFDIVLHKLLGEEWREIIEVCKLHSLQPTFTLYL*