AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006514.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006514.01.T01 MTR_2g009800 81.081 555 7 3 1 457 1 555 0.0 872
MsG0280006514.01.T01 MTR_2g101620 75.135 555 40 3 1 457 1 555 0.0 812
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006514.01.T01 AT5G14050 50.628 557 164 10 1 457 1 546 9.12e-173 496

Find 89 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTGGGCGCAGCTGGATTC+AGG 0.199184 2:+2946220 MsG0280006514.01.T01:CDS
ATGGAACTGAACTGAATTAA+TGG 0.221155 2:-2946443 None:intergenic
CTGAGAAGTCAGCATCTTCT+TGG 0.323321 2:-2946004 None:intergenic
AAAGCTATCATTTGAGAATC+AGG 0.336697 2:-2946733 None:intergenic
AAGGAAGATACTTTCAATCT+TGG 0.340340 2:-2946542 None:intergenic
GAAGAGTATTGAAGTGTTTG+AGG 0.342182 2:+2946705 MsG0280006514.01.T01:CDS
AGATTATCAATGGTCTTGAT+AGG 0.358024 2:-2947071 None:intergenic
TACTTCAGGCATGGTGATAA+TGG 0.362722 2:-2947328 None:intergenic
GGTCAGCTGCTTCTTGCTGC+TGG 0.365476 2:+2946472 MsG0280006514.01.T01:CDS
AGGAACTGACTGGGCGCAGC+TGG 0.391668 2:+2946213 MsG0280006514.01.T01:CDS
TGGGCTAAAATCAATACAAC+GGG 0.399262 2:-2947246 None:intergenic
TGGAACTGAACTGAATTAAT+GGG 0.402013 2:-2946442 None:intergenic
GGGTCTCAGGTGATAATATC+AGG 0.411556 2:+2946604 MsG0280006514.01.T01:CDS
TTTCTTGCCCGATGGGTCTC+AGG 0.414581 2:+2946591 MsG0280006514.01.T01:CDS
ATGGCTGTAGGAAATGCTTC+AGG 0.420095 2:+2947281 MsG0280006514.01.T01:CDS
TTATAGTCTTGATTTAGTTA+AGG 0.420893 2:+2946642 MsG0280006514.01.T01:CDS
TTCGAATGGGGCAATCTTCA+AGG 0.424695 2:-2946561 None:intergenic
TCAGTTCAGTTCCATCGAAA+TGG 0.438541 2:+2946451 MsG0280006514.01.T01:CDS
AAATACTAGCAATCTGTTCA+AGG 0.442805 2:+2947135 MsG0280006514.01.T01:CDS
TTCAGTATTCAGATTATCAA+TGG 0.445525 2:-2947081 None:intergenic
AATATACAAGATCCCGCTAA+TGG 0.453091 2:+2946418 MsG0280006514.01.T01:CDS
TACAATAGCGAGGAATTTCT+CGG 0.460074 2:+2947035 MsG0280006514.01.T01:CDS
GAGCCGAGGTTACGAGGATC+TGG 0.461776 2:+2946303 MsG0280006514.01.T01:CDS
AAGGCTAGAGTTGATAAGAT+TGG 0.470242 2:+2946661 MsG0280006514.01.T01:CDS
AGCTCAACCGAGGAACTGAC+TGG 0.472580 2:+2946203 MsG0280006514.01.T01:CDS
GATAAGATTGGTCCGCTGTT+GGG 0.475592 2:+2946673 MsG0280006514.01.T01:CDS
ACTGGGCGCAGCTGGATTCA+GGG 0.478998 2:+2946221 MsG0280006514.01.T01:CDS
AAGCATCTTTCTTGCCCGAT+GGG 0.479466 2:+2946584 MsG0280006514.01.T01:CDS
TTTGCAGCTGGTTCAGCTAG+TGG 0.484592 2:+2946999 MsG0280006514.01.T01:CDS
AAAGCCGGTGTGGGTGGATG+AGG 0.489666 2:+2946058 MsG0280006514.01.T01:CDS
ACAAAGTCGAAGCAATTAGT+TGG 0.498298 2:+2946790 MsG0280006514.01.T01:CDS
ACAATAGCGAGGAATTTCTC+GGG 0.503579 2:+2947036 MsG0280006514.01.T01:CDS
GAATTAATGGGACCATTAGC+GGG 0.514356 2:-2946430 None:intergenic
TGATAAGATTGGTCCGCTGT+TGG 0.514998 2:+2946672 MsG0280006514.01.T01:CDS
GATTGGTCCGCTGTTGGGTA+GGG 0.516250 2:+2946678 MsG0280006514.01.T01:CDS
GGATGCCGCGGCTTCTGCAA+AGG 0.518753 2:+2945929 MsG0280006514.01.T01:CDS
GCTGCGCCCAGTCAGTTCCT+CGG 0.520974 2:-2946210 None:intergenic
TGAATTAATGGGACCATTAG+CGG 0.525771 2:-2946431 None:intergenic
GGTGGATGAGGAAGAAGAAA+AGG 0.532095 2:+2946070 MsG0280006514.01.T01:CDS
TTCGAGTATTCAAGATGTCC+TGG 0.533881 2:-2946382 None:intergenic
AAAGGAAATGAGGTTGAAGA+TGG 0.535620 2:+2945742 MsG0280006514.01.T01:CDS
AGATTGGTCCGCTGTTGGGT+AGG 0.539038 2:+2946677 MsG0280006514.01.T01:CDS
ATTATCACCTGAGACCCATC+GGG 0.550131 2:-2946598 None:intergenic
AGATGGAAAGGAAGAACAGT+TGG 0.550771 2:+2945810 MsG0280006514.01.T01:CDS
TTGAGCTACTCTTCACAACC+AGG 0.551752 2:-2946351 None:intergenic
CAATACAACGGGGATAATGC+AGG 0.553209 2:-2947235 None:intergenic
AAAGAGGAAGCTAGATGGAA+AGG 0.553316 2:+2945798 MsG0280006514.01.T01:CDS
TGATAATCTGAATACTGAAG+TGG 0.554817 2:+2947085 MsG0280006514.01.T01:CDS
AAAGCATCTTTCTTGCCCGA+TGG 0.557386 2:+2946583 MsG0280006514.01.T01:CDS
TTCTTGCTGCTGGATTAGAC+CGG 0.558175 2:+2946482 MsG0280006514.01.T01:CDS
TATTATCACCTGAGACCCAT+CGG 0.558404 2:-2946599 None:intergenic
AAATGAGGTTGAAGATGGGA+AGG 0.558756 2:+2945747 MsG0280006514.01.T01:CDS
AAGGAAATGAGGTTGAAGAT+GGG 0.562237 2:+2945743 MsG0280006514.01.T01:CDS
AGTAGCTCAAAATTGTTGCC+AGG 0.564255 2:+2946364 MsG0280006514.01.T01:CDS
GGAGTTTGATGTTGATACTG+AGG 0.566399 2:+2945768 MsG0280006514.01.T01:CDS
TGCTGTGAGCCGAGGTTACG+AGG 0.576924 2:+2946297 MsG0280006514.01.T01:CDS
CATCCAGATCCTCGTAACCT+CGG 0.587962 2:-2946306 None:intergenic
AATACTAGCAATCTGTTCAA+GGG 0.593971 2:+2947136 MsG0280006514.01.T01:CDS
GGAAGTTGTGAAAGGAAATG+AGG 0.594864 2:+2945732 MsG0280006514.01.T01:CDS
TTTCTGATCTACTTCAGGCA+TGG 0.600646 2:-2947337 None:intergenic
GAAGCAGCTGACCATTTCGA+TGG 0.604108 2:-2946462 None:intergenic
CTTCAGTAAAAGCTAATGAA+CGG 0.605560 2:-2946837 None:intergenic
TGTGAATATTTACAATAGCG+AGG 0.608077 2:+2947025 MsG0280006514.01.T01:CDS
AAAAGGGAGGAAGTTGTGAA+AGG 0.616065 2:+2945724 MsG0280006514.01.T01:CDS
AATAAAGCTGCTGTGAGCCG+AGG 0.616455 2:+2946289 MsG0280006514.01.T01:CDS
AGAAGCCTTTGCAGAAGCCG+CGG 0.617253 2:-2945934 None:intergenic
TCTCAGAACAAATGAAGACC+TGG 0.621951 2:+2946333 MsG0280006514.01.T01:CDS
CTGGGCTAAAATCAATACAA+CGG 0.628701 2:-2947247 None:intergenic
ACAGCCATAAAACCACCACC+TGG 0.630227 2:-2947266 None:intergenic
TGATGATGAGGTGGATGCCG+CGG 0.633167 2:+2945917 MsG0280006514.01.T01:intron
AAAGGAAGAACAGTTGGCAA+AGG 0.637301 2:+2945816 MsG0280006514.01.T01:CDS
GCTCAACCGAGGAACTGACT+GGG 0.639373 2:+2946204 MsG0280006514.01.T01:CDS
CCAGTTAGAGAAAACAGTGT+AGG 0.639938 2:-2947195 None:intergenic
GTTGGTACTCTGAAGATGAA+CGG 0.640176 2:+2946808 MsG0280006514.01.T01:CDS
TTAGAGAAAACAGTGTAGGA+TGG 0.640295 2:-2947191 None:intergenic
CAATAGCGAGGAATTTCTCG+GGG 0.640898 2:+2947037 MsG0280006514.01.T01:CDS
GAAAAGAAAGCCGGTGTGGG+TGG 0.643363 2:+2946052 MsG0280006514.01.T01:CDS
TGCAGGCTCGCATTTGAACG+CGG 0.645712 2:-2947218 None:intergenic
TCTTCCTCATCCACCCACAC+CGG 0.648855 2:-2946062 None:intergenic
CTTTGAAAAGAAAGCCGGTG+TGG 0.649038 2:+2946048 MsG0280006514.01.T01:CDS
TTCTCTTCCCTACCCAACAG+CGG 0.649632 2:-2946685 None:intergenic
TGATGCTTTGAAAAGAAAGC+CGG 0.651576 2:+2946043 MsG0280006514.01.T01:CDS
CAGCCATAAAACCACCACCT+GGG 0.668450 2:-2947265 None:intergenic
TGAGGAGACTATCTGCAGAA+CGG 0.681037 2:-2945974 None:intergenic
CATCTTCTTGGTAATCAGTG+AGG 0.681529 2:-2945992 None:intergenic
TTTGAAAAGAAAGCCGGTGT+GGG 0.693980 2:+2946049 MsG0280006514.01.T01:CDS
CAGCATGCAAAGCTCAACCG+AGG 0.712589 2:+2946193 MsG0280006514.01.T01:intron
AATAGCGAGGAATTTCTCGG+GGG 0.758971 2:+2947038 MsG0280006514.01.T01:CDS
GGGCTAAAATCAATACAACG+GGG 0.814054 2:-2947245 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAAATTTTTTCAAATTGA+TGG + Chr2:2946508-2946527 MsG0280006514.01.T01:CDS 15.0%
!! TTTGAAAAAATTTAAGCTTC+CGG - Chr2:2946504-2946523 None:intergenic 20.0%
!!! TTATAGTCTTGATTTAGTTA+AGG + Chr2:2946642-2946661 MsG0280006514.01.T01:CDS 20.0%
! TTCAGTATTCAGATTATCAA+TGG - Chr2:2947084-2947103 None:intergenic 25.0%
!! CAATGAAGGTTATATTTTAC+TGG + Chr2:2946762-2946781 MsG0280006514.01.T01:CDS 25.0%
!!! TTTTTCAAATTGATGGCAAA+CGG + Chr2:2946515-2946534 MsG0280006514.01.T01:CDS 25.0%
AAAGCTATCATTTGAGAATC+AGG - Chr2:2946736-2946755 None:intergenic 30.0%
AAATACTAGCAATCTGTTCA+AGG + Chr2:2947135-2947154 MsG0280006514.01.T01:CDS 30.0%
AAGGAAGATACTTTCAATCT+TGG - Chr2:2946545-2946564 None:intergenic 30.0%
AATACTAGCAATCTGTTCAA+GGG + Chr2:2947136-2947155 MsG0280006514.01.T01:CDS 30.0%
ATGGAACTGAACTGAATTAA+TGG - Chr2:2946446-2946465 None:intergenic 30.0%
CTTCAGTAAAAGCTAATGAA+CGG - Chr2:2946840-2946859 None:intergenic 30.0%
TCAACAGATTAAGGAAGTTA+AGG + Chr2:2946114-2946133 MsG0280006514.01.T01:intron 30.0%
TGATAATCTGAATACTGAAG+TGG + Chr2:2947085-2947104 MsG0280006514.01.T01:CDS 30.0%
TGATGGTCATATTTACCATT+GGG + Chr2:2946888-2946907 MsG0280006514.01.T01:intron 30.0%
TGGAACTGAACTGAATTAAT+GGG - Chr2:2946445-2946464 None:intergenic 30.0%
TGTGAATATTTACAATAGCG+AGG + Chr2:2947025-2947044 MsG0280006514.01.T01:CDS 30.0%
! AAGAAAGATGCTTTTCGAAT+GGG - Chr2:2946577-2946596 None:intergenic 30.0%
! AGATTATCAATGGTCTTGAT+AGG - Chr2:2947074-2947093 None:intergenic 30.0%
! CTCTAAAAATGAGACGAAAA+GGG + Chr2:2945708-2945727 MsG0280006514.01.T01:CDS 30.0%
! TCTCAAATGATAGCTTTTGT+GGG + Chr2:2946739-2946758 MsG0280006514.01.T01:CDS 30.0%
! TTCTCAAATGATAGCTTTTG+TGG + Chr2:2946738-2946757 MsG0280006514.01.T01:CDS 30.0%
!!! TCATTAGCTTTTACTGAAGA+TGG + Chr2:2946841-2946860 MsG0280006514.01.T01:CDS 30.0%
AAAGGAAATGAGGTTGAAGA+TGG + Chr2:2945742-2945761 MsG0280006514.01.T01:CDS 35.0%
AAGGAAATGAGGTTGAAGAT+GGG + Chr2:2945743-2945762 MsG0280006514.01.T01:CDS 35.0%
AATATACAAGATCCCGCTAA+TGG + Chr2:2946418-2946437 MsG0280006514.01.T01:CDS 35.0%
ACAAAGTCGAAGCAATTAGT+TGG + Chr2:2946790-2946809 MsG0280006514.01.T01:CDS 35.0%
ACAAGGACTTGTATACACAA+AGG + Chr2:2946919-2946938 MsG0280006514.01.T01:intron 35.0%
AGAGTATGTGTCAAGATTGA+GGG + Chr2:2946168-2946187 MsG0280006514.01.T01:intron 35.0%
ATACTGAGGAAACGAAAAAG+AGG + Chr2:2945782-2945801 MsG0280006514.01.T01:CDS 35.0%
CAAGGACTTGTATACACAAA+GGG + Chr2:2946920-2946939 MsG0280006514.01.T01:intron 35.0%
CAGATTAAGGAAGTTAAGGA+AGG + Chr2:2946118-2946137 MsG0280006514.01.T01:intron 35.0%
CTGGGCTAAAATCAATACAA+CGG - Chr2:2947250-2947269 None:intergenic 35.0%
GTAGATGAAGGCTGTTTAAA+CGG + Chr2:2946942-2946961 MsG0280006514.01.T01:intron 35.0%
GTGATGGTCATATTTACCAT+TGG + Chr2:2946887-2946906 MsG0280006514.01.T01:intron 35.0%
TAAAAATGAGACGAAAAGGG+AGG + Chr2:2945711-2945730 MsG0280006514.01.T01:CDS 35.0%
TACAATAGCGAGGAATTTCT+CGG + Chr2:2947035-2947054 MsG0280006514.01.T01:CDS 35.0%
TAGCCAAAGTCAACAGATTA+AGG + Chr2:2946105-2946124 MsG0280006514.01.T01:intron 35.0%
TATACACAAAGGGTAGATGA+AGG + Chr2:2946930-2946949 MsG0280006514.01.T01:intron 35.0%
TGAATTAATGGGACCATTAG+CGG - Chr2:2946434-2946453 None:intergenic 35.0%
TGGGCTAAAATCAATACAAC+GGG - Chr2:2947249-2947268 None:intergenic 35.0%
TTAGAGAAAACAGTGTAGGA+TGG - Chr2:2947194-2947213 None:intergenic 35.0%
! AAGCTTGAGAGCTTTTTGTT+TGG + Chr2:2945859-2945878 MsG0280006514.01.T01:intron 35.0%
! AGAAAGATGCTTTTCGAATG+GGG - Chr2:2946576-2946595 None:intergenic 35.0%
! CAAGAAAGATGCTTTTCGAA+TGG - Chr2:2946578-2946597 None:intergenic 35.0%
!! AAGGCTAGAGTTGATAAGAT+TGG + Chr2:2946661-2946680 MsG0280006514.01.T01:CDS 35.0%
!! AGCTTGAGAGCTTTTTGTTT+GGG + Chr2:2945860-2945879 MsG0280006514.01.T01:intron 35.0%
!! CTTCCTTAATCTGTTGACTT+TGG - Chr2:2946111-2946130 None:intergenic 35.0%
!! GAAGAGTATTGAAGTGTTTG+AGG + Chr2:2946705-2946724 MsG0280006514.01.T01:CDS 35.0%
!! GCTCTAAAAATGAGACGAAA+AGG + Chr2:2945707-2945726 MsG0280006514.01.T01:CDS 35.0%
!! TGATGCTTTGAAAAGAAAGC+CGG + Chr2:2946043-2946062 MsG0280006514.01.T01:CDS 35.0%
AAAAGGGAGGAAGTTGTGAA+AGG + Chr2:2945724-2945743 MsG0280006514.01.T01:CDS 40.0%
AAAGAGGAAGCTAGATGGAA+AGG + Chr2:2945798-2945817 MsG0280006514.01.T01:CDS 40.0%
AAAGGAAGAACAGTTGGCAA+AGG + Chr2:2945816-2945835 MsG0280006514.01.T01:CDS 40.0%
AAATGAGGTTGAAGATGGGA+AGG + Chr2:2945747-2945766 MsG0280006514.01.T01:CDS 40.0%
ACAATAGCGAGGAATTTCTC+GGG + Chr2:2947036-2947055 MsG0280006514.01.T01:CDS 40.0%
ACGAAAAAGAGGAAGCTAGA+TGG + Chr2:2945793-2945812 MsG0280006514.01.T01:CDS 40.0%
AGATGGAAAGGAAGAACAGT+TGG + Chr2:2945810-2945829 MsG0280006514.01.T01:CDS 40.0%
AGTAGCTCAAAATTGTTGCC+AGG + Chr2:2946364-2946383 MsG0280006514.01.T01:CDS 40.0%
ATCATCCCCTTTCCAAATTC+AGG - Chr2:2945901-2945920 None:intergenic 40.0%
CAGAGTATGTGTCAAGATTG+AGG + Chr2:2946167-2946186 MsG0280006514.01.T01:intron 40.0%
CCAGTTAGAGAAAACAGTGT+AGG - Chr2:2947198-2947217 None:intergenic 40.0%
CTCTATTCACCTCCTGAATT+TGG + Chr2:2945886-2945905 MsG0280006514.01.T01:intron 40.0%
CTCTTTGTACTTCTCCAGTA+GGG + Chr2:2946970-2946989 MsG0280006514.01.T01:intron 40.0%
GAATTAATGGGACCATTAGC+GGG - Chr2:2946433-2946452 None:intergenic 40.0%
GAGGATGAGGATTTGATATC+TGG + Chr2:2946142-2946161 MsG0280006514.01.T01:intron 40.0%
GATTCAGGGTCAAAAATGGA+TGG + Chr2:2946235-2946254 MsG0280006514.01.T01:CDS 40.0%
GGAAGTTGTGAAAGGAAATG+AGG + Chr2:2945732-2945751 MsG0280006514.01.T01:CDS 40.0%
GGACAAAAATTGTTAAGCGC+TGG + Chr2:2946862-2946881 MsG0280006514.01.T01:CDS 40.0%
GGAGTTTGATGTTGATACTG+AGG + Chr2:2945768-2945787 MsG0280006514.01.T01:CDS 40.0%
GGGCTAAAATCAATACAACG+GGG - Chr2:2947248-2947267 None:intergenic 40.0%
TAAGGAAGTTAAGGAAGGAG+AGG + Chr2:2946123-2946142 MsG0280006514.01.T01:intron 40.0%
TATTATCACCTGAGACCCAT+CGG - Chr2:2946602-2946621 None:intergenic 40.0%
TCACCTCCTGAATTTGGAAA+GGG + Chr2:2945892-2945911 MsG0280006514.01.T01:intron 40.0%
TCAGTTCAGTTCCATCGAAA+TGG + Chr2:2946451-2946470 MsG0280006514.01.T01:CDS 40.0%
TCTCAGAACAAATGAAGACC+TGG + Chr2:2946333-2946352 MsG0280006514.01.T01:CDS 40.0%
TTCACCTCCTGAATTTGGAA+AGG + Chr2:2945891-2945910 MsG0280006514.01.T01:intron 40.0%
TTCGAGTATTCAAGATGTCC+TGG - Chr2:2946385-2946404 None:intergenic 40.0%
TTTGAAAAGAAAGCCGGTGT+GGG + Chr2:2946049-2946068 MsG0280006514.01.T01:CDS 40.0%
! ATAGCTTTTGTGGGCAATGA+AGG + Chr2:2946748-2946767 MsG0280006514.01.T01:CDS 40.0%
! CATCTTCTTGGTAATCAGTG+AGG - Chr2:2945995-2946014 None:intergenic 40.0%
! CCTACACTGTTTTCTCTAAC+TGG + Chr2:2947195-2947214 MsG0280006514.01.T01:CDS 40.0%
! GTTGGTACTCTGAAGATGAA+CGG + Chr2:2946808-2946827 MsG0280006514.01.T01:CDS 40.0%
!! CAAAAATTGTTAAGCGCTGG+TGG + Chr2:2946865-2946884 MsG0280006514.01.T01:CDS 40.0%
!!! CGTTGTATTGATTTTAGCCC+AGG + Chr2:2947248-2947267 MsG0280006514.01.T01:CDS 40.0%
!!! TGTATTGATTTTAGCCCAGG+TGG + Chr2:2947251-2947270 MsG0280006514.01.T01:CDS 40.0%
AAGCATCTTTCTTGCCCGAT+GGG + Chr2:2946584-2946603 MsG0280006514.01.T01:CDS 45.0%
AATAGCGAGGAATTTCTCGG+GGG + Chr2:2947038-2947057 MsG0280006514.01.T01:CDS 45.0%
ACCATTGGGATCTGAGAACA+AGG + Chr2:2946902-2946921 MsG0280006514.01.T01:intron 45.0%
AGTTAAGGAAGGAGAGGATG+AGG + Chr2:2946129-2946148 MsG0280006514.01.T01:intron 45.0%
ATCCCCTTTCCAAATTCAGG+AGG - Chr2:2945898-2945917 None:intergenic 45.0%
ATGGCTGTAGGAAATGCTTC+AGG + Chr2:2947281-2947300 MsG0280006514.01.T01:CDS 45.0%
ATTATCACCTGAGACCCATC+GGG - Chr2:2946601-2946620 None:intergenic 45.0%
CAATACAACGGGGATAATGC+AGG - Chr2:2947238-2947257 None:intergenic 45.0%
CAATAGCGAGGAATTTCTCG+GGG + Chr2:2947037-2947056 MsG0280006514.01.T01:CDS 45.0%
CACCTCCTGAATTTGGAAAG+GGG + Chr2:2945893-2945912 MsG0280006514.01.T01:intron 45.0%
CTGAGAAGTCAGCATCTTCT+TGG - Chr2:2946007-2946026 None:intergenic 45.0%
GCTCTTTGTACTTCTCCAGT+AGG + Chr2:2946969-2946988 MsG0280006514.01.T01:intron 45.0%
GCTGGATTCAGGGTCAAAAA+TGG + Chr2:2946231-2946250 MsG0280006514.01.T01:CDS 45.0%
GGGTCTCAGGTGATAATATC+AGG + Chr2:2946604-2946623 MsG0280006514.01.T01:CDS 45.0%
GGTGGATGAGGAAGAAGAAA+AGG + Chr2:2946070-2946089 MsG0280006514.01.T01:CDS 45.0%
TCCTTGTTCTCAGATCCCAA+TGG - Chr2:2946906-2946925 None:intergenic 45.0%
TGAGGAGACTATCTGCAGAA+CGG - Chr2:2945977-2945996 None:intergenic 45.0%
TTCGAATGGGGCAATCTTCA+AGG - Chr2:2946564-2946583 None:intergenic 45.0%
TTGAGCTACTCTTCACAACC+AGG - Chr2:2946354-2946373 None:intergenic 45.0%
! AAAGCATCTTTCTTGCCCGA+TGG + Chr2:2946583-2946602 MsG0280006514.01.T01:CDS 45.0%
! CTTTGAAAAGAAAGCCGGTG+TGG + Chr2:2946048-2946067 MsG0280006514.01.T01:CDS 45.0%
! GATAAGATTGGTCCGCTGTT+GGG + Chr2:2946673-2946692 MsG0280006514.01.T01:CDS 45.0%
! TGATAAGATTGGTCCGCTGT+TGG + Chr2:2946672-2946691 MsG0280006514.01.T01:CDS 45.0%
! TTCTTGCTGCTGGATTAGAC+CGG + Chr2:2946482-2946501 MsG0280006514.01.T01:CDS 45.0%
! TTGGAAAGGGGATGATGATG+AGG + Chr2:2945905-2945924 MsG0280006514.01.T01:intron 45.0%
!! ATTGATTTTAGCCCAGGTGG+TGG + Chr2:2947254-2947273 MsG0280006514.01.T01:CDS 45.0%
AATAAAGCTGCTGTGAGCCG+AGG + Chr2:2946289-2946308 MsG0280006514.01.T01:CDS 50.0%
ACAGCCATAAAACCACCACC+TGG - Chr2:2947269-2947288 None:intergenic 50.0%
CAGCCATAAAACCACCACCT+GGG - Chr2:2947268-2947287 None:intergenic 50.0%
CATCCAGATCCTCGTAACCT+CGG - Chr2:2946309-2946328 None:intergenic 50.0%
GAAGCAGCTGACCATTTCGA+TGG - Chr2:2946465-2946484 None:intergenic 50.0%
GCTGCAAAATGTGTCCCTAC+TGG - Chr2:2946987-2947006 None:intergenic 50.0%
TTCTCTTCCCTACCCAACAG+CGG - Chr2:2946688-2946707 None:intergenic 50.0%
! GAAAGGGGATGATGATGAGG+TGG + Chr2:2945908-2945927 MsG0280006514.01.T01:intron 50.0%
! GTAGGGACACATTTTGCAGC+TGG + Chr2:2946987-2947006 MsG0280006514.01.T01:intron 50.0%
! TTTGCAGCTGGTTCAGCTAG+TGG + Chr2:2946999-2947018 MsG0280006514.01.T01:CDS 50.0%
!! TAGCCCAGGTGGTGGTTTTA+TGG + Chr2:2947262-2947281 MsG0280006514.01.T01:CDS 50.0%
!! TTGTTAAGCGCTGGTGGTGA+TGG + Chr2:2946871-2946890 MsG0280006514.01.T01:intron 50.0%
!!! GGTGGTGGTTTTATGGCTGT+AGG + Chr2:2947269-2947288 MsG0280006514.01.T01:CDS 50.0%
AGAAGCCTTTGCAGAAGCCG+CGG - Chr2:2945937-2945956 None:intergenic 55.0%
AGCTCAACCGAGGAACTGAC+TGG + Chr2:2946203-2946222 MsG0280006514.01.T01:CDS 55.0%
CAGCATGCAAAGCTCAACCG+AGG + Chr2:2946193-2946212 MsG0280006514.01.T01:intron 55.0%
GAAAAGAAAGCCGGTGTGGG+TGG + Chr2:2946052-2946071 MsG0280006514.01.T01:CDS 55.0%
GCTCAACCGAGGAACTGACT+GGG + Chr2:2946204-2946223 MsG0280006514.01.T01:CDS 55.0%
TCTTCCTCATCCACCCACAC+CGG - Chr2:2946065-2946084 None:intergenic 55.0%
TGCAGGCTCGCATTTGAACG+CGG - Chr2:2947221-2947240 None:intergenic 55.0%
TTTCTTGCCCGATGGGTCTC+AGG + Chr2:2946591-2946610 MsG0280006514.01.T01:CDS 55.0%
! AGATTGGTCCGCTGTTGGGT+AGG + Chr2:2946677-2946696 MsG0280006514.01.T01:CDS 55.0%
! GATTGGTCCGCTGTTGGGTA+GGG + Chr2:2946678-2946697 MsG0280006514.01.T01:CDS 55.0%
! TGATGATGAGGTGGATGCCG+CGG + Chr2:2945917-2945936 MsG0280006514.01.T01:intron 55.0%
AAAGCCGGTGTGGGTGGATG+AGG + Chr2:2946058-2946077 MsG0280006514.01.T01:CDS 60.0%
ACTGGGCGCAGCTGGATTCA+GGG + Chr2:2946221-2946240 MsG0280006514.01.T01:CDS 60.0%
GAGCCGAGGTTACGAGGATC+TGG + Chr2:2946303-2946322 MsG0280006514.01.T01:CDS 60.0%
GGTCAGCTGCTTCTTGCTGC+TGG + Chr2:2946472-2946491 MsG0280006514.01.T01:CDS 60.0%
TGCTGTGAGCCGAGGTTACG+AGG + Chr2:2946297-2946316 MsG0280006514.01.T01:CDS 60.0%
AGGAACTGACTGGGCGCAGC+TGG + Chr2:2946213-2946232 MsG0280006514.01.T01:CDS 65.0%
GACTGGGCGCAGCTGGATTC+AGG + Chr2:2946220-2946239 MsG0280006514.01.T01:CDS 65.0%
GCTGCGCCCAGTCAGTTCCT+CGG - Chr2:2946213-2946232 None:intergenic 65.0%
GGATGCCGCGGCTTCTGCAA+AGG + Chr2:2945929-2945948 MsG0280006514.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 2945682 2947346 2945682 ID=MsG0280006514.01;Name=MsG0280006514.01
Chr2 mRNA 2945682 2947346 2945682 ID=MsG0280006514.01.T01;Parent=MsG0280006514.01;Name=MsG0280006514.01.T01;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|1|1|4|0|457
Chr2 exon 2945682 2945843 2945682 ID=MsG0280006514.01.T01:exon:1575;Parent=MsG0280006514.01.T01
Chr2 exon 2945927 2946091 2945927 ID=MsG0280006514.01.T01:exon:1576;Parent=MsG0280006514.01.T01
Chr2 exon 2946196 2946886 2946196 ID=MsG0280006514.01.T01:exon:1577;Parent=MsG0280006514.01.T01
Chr2 exon 2946991 2947346 2946991 ID=MsG0280006514.01.T01:exon:1578;Parent=MsG0280006514.01.T01
Chr2 CDS 2945682 2945843 2945682 ID=MsG0280006514.01.T01:cds;Parent=MsG0280006514.01.T01
Chr2 CDS 2945927 2946091 2945927 ID=MsG0280006514.01.T01:cds;Parent=MsG0280006514.01.T01
Chr2 CDS 2946196 2946886 2946196 ID=MsG0280006514.01.T01:cds;Parent=MsG0280006514.01.T01
Chr2 CDS 2946991 2947346 2946991 ID=MsG0280006514.01.T01:cds;Parent=MsG0280006514.01.T01
Gene Sequence

>MsG0280006514.01.T01

ATGAGTTTAATATCTCAAAATGCTCGCTCTAAAAATGAGACGAAAAGGGAGGAAGTTGTGAAAGGAAATGAGGTTGAAGATGGGAAGGAGTTTGATGTTGATACTGAGGAAACGAAAAAGAGGAAGCTAGATGGAAAGGAAGAACAGTTGGCAAAGGAACAAGTGGATGCCGCGGCTTCTGCAAAGGCTTCTAATTTGTTCTTCACTGACCGTTCTGCAGATAGTCTCCTCACTGATTACCAAGAAGATGCTGACTTCTCAGATGAAAGTGATAATGATGATGCTTTGAAAAGAAAGCCGGTGTGGGTGGATGAGGAAGAAGAAAAGCATGCAAAGCTCAACCGAGGAACTGACTGGGCGCAGCTGGATTCAGGGTCAAAAATGGATGGATCTTCTGATGATGAATTGACAGATGATGAAAATAAAGCTGCTGTGAGCCGAGGTTACGAGGATCTGGATGATATTCTCAGAACAAATGAAGACCTGGTTGTGAAGAGTAGCTCAAAATTGTTGCCAGGACATCTTGAATACTCGAAGCTTGTCGATGCAAATATACAAGATCCCGCTAATGGTCCCATTAATTCAGTTCAGTTCCATCGAAATGGTCAGCTGCTTCTTGCTGCTGGATTAGACCGGAAGCTTAAATTTTTTCAAATTGATGGCAAACGGAACACCAAGATTGAAAGTATCTTCCTTGAAGATTGCCCCATTCGAAAAGCATCTTTCTTGCCCGATGGGTCTCAGGTGATAATATCAGGAAGAAGAAAGTTCTTTTATAGTCTTGATTTAGTTAAGGCTAGAGTTGATAAGATTGGTCCGCTGTTGGGTAGGGAAGAGAAGAGTATTGAAGTGTTTGAGGTCTCTCCTGATTCTCAAATGATAGCTTTTGTGGGCAATGAAGGTTATATTTTACTGGTTTCAACAAAGTCGAAGCAATTAGTTGGTACTCTGAAGATGAACGGAACAGTCCGTTCATTAGCTTTTACTGAAGATGGACAAAAATTGTTAAGCGCTGGTGGGACACATTTTGCAGCTGGTTCAGCTAGTGGAGTTGTGAATATTTACAATAGCGAGGAATTTCTCGGGGGAAAGAGAAAGCCTATCAAGACCATTGATAATCTGAATACTGAAGTGGATTTCATGAAATTCAATCATGACTCTCAAATACTAGCAATCTGTTCAAGGGCGAAGAACAGCAGTTTGAAATTAGTACATATACCATCCTACACTGTTTTCTCTAACTGGCCGCGTTCAAATGCGAGCCTGCATTATCCCCGTTGTATTGATTTTAGCCCAGGTGGTGGTTTTATGGCTGTAGGAAATGCTTCAGGCAAAGTGTTACTGTATAAGTTGCACCATTATCACCATGCCTGA
Protein sequence

>MsG0280006514.01.T01

MSLISQNARSKNETKREEVVKGNEVEDGKEFDVDTEETKKRKLDGKEEQLAKEQVDAAASAKASNLFFTDRSADSLLTDYQEDADFSDESDNDDALKRKPVWVDEEEEKHAKLNRGTDWAQLDSGSKMDGSSDDELTDDENKAAVSRGYEDLDDILRTNEDLVVKSSSKLLPGHLEYSKLVDANIQDPANGPINSVQFHRNGQLLLAAGLDRKLKFFQIDGKRNTKIESIFLEDCPIRKASFLPDGSQVIISGRRKFFYSLDLVKARVDKIGPLLGREEKSIEVFEVSPDSQMIAFVGNEGYILLVSTKSKQLVGTLKMNGTVRSLAFTEDGQKLLSAGGTHFAAGSASGVVNIYNSEEFLGGKRKPIKTIDNLNTEVDFMKFNHDSQILAICSRAKNSSLKLVHIPSYTVFSNWPRSNASLHYPRCIDFSPGGGFMAVGNASGKVLLYKLHHYHHA*