AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007445.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007445.01.T01 MTR_3g096190 96.842 190 6 0 4 193 93 282 8.58e-139 389
MsG0280007445.01.T01 MTR_4g032390 95.789 190 8 0 4 193 95 284 3.90e-136 382
MsG0280007445.01.T01 MTR_3g096190 97.414 116 3 0 4 119 93 208 1.15e-80 239
MsG0280007445.01.T01 MTR_5g464770 52.217 203 86 4 2 193 37 239 1.54e-68 209
MsG0280007445.01.T01 MTR_1g112880 54.118 170 77 1 24 193 2 170 2.02e-62 191
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007445.01.T01 AT3G57000 68.229 192 59 2 4 193 107 298 4.99e-97 283

Find 49 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGCTTTGATACCAATT+AGG 0.201791 2:+15571900 None:intergenic
AGGAACTCTTGTTCCCTAAT+TGG 0.233083 2:-15571914 MsG0280007445.01.T01:three_prime_UTR
GCGAACCGTTTGAATGTTCT+TGG 0.244591 2:+15573432 None:intergenic
ATACAGTAAGCAGCACTTAA+AGG 0.273684 2:+15572156 None:intergenic
GAGAGTTGACGGGCAAATAC+TGG 0.282558 2:+15573189 None:intergenic
AAATGCTTTGATACCAATTA+GGG 0.323531 2:+15571901 None:intergenic
ACGGGCAAATACTGGGTTAC+GGG 0.353988 2:+15573197 None:intergenic
TAAAGCTGGGAGGTTGAAAA+TGG 0.355073 2:-15573511 MsG0280007445.01.T01:CDS
CAATCAGGACCTTGTCTTTG+TGG 0.379821 2:-15572711 MsG0280007445.01.T01:intron
TTAGCTTCTTCTATTTATGC+TGG 0.390838 2:-15572086 MsG0280007445.01.T01:three_prime_UTR
CAGGATTCTTGCTATGTTTG+CGG 0.403073 2:+15576108 None:intergenic
GTCAACAATTCCAAGCAATC+AGG 0.407962 2:-15572726 MsG0280007445.01.T01:CDS
GTATACATAAGGACTGAAAA+AGG 0.415050 2:-15573489 MsG0280007445.01.T01:CDS
TACAGTAAGCAGCACTTAAA+GGG 0.420498 2:+15572157 None:intergenic
CAGACCTGACATTTGTCATC+AGG 0.431148 2:-15576078 MsG0280007445.01.T01:intron
ACCTCCCAGCTTTATTTAGC+GGG 0.432591 2:+15573520 None:intergenic
CTTCTTCTATTTATGCTGGA+TGG 0.447976 2:-15572082 MsG0280007445.01.T01:three_prime_UTR
AAGGATTACCAATGCTATTG+AGG 0.448693 2:-15572130 MsG0280007445.01.T01:CDS
ATCTCTATCTTCTACATCAT+AGG 0.452246 2:-15571934 MsG0280007445.01.T01:three_prime_UTR
CCCGTCAACTCTCGAAAAGC+AGG 0.471030 2:-15573179 MsG0280007445.01.T01:intron
AGGATTACCAATGCTATTGA+GGG 0.471656 2:-15572129 MsG0280007445.01.T01:CDS
GATAGCCCGCTAAATAAAGC+TGG 0.474848 2:-15573525 MsG0280007445.01.T01:CDS
CCACTTTCCCTCAATAGCAT+TGG 0.478446 2:+15572122 None:intergenic
TACACAGATGATTATATAGC+TGG 0.482001 2:-15572574 MsG0280007445.01.T01:intron
AAGACAAGGTCCTGATTGCT+TGG 0.489499 2:+15572716 None:intergenic
AAATTGAGTATAACTGCTGC+TGG 0.491203 2:-15573254 MsG0280007445.01.T01:CDS
AACCTCCCAGCTTTATTTAG+CGG 0.492235 2:+15573519 None:intergenic
GTTCTTGGAATGCGAACATA+GGG 0.511003 2:+15573447 None:intergenic
GGTTGAAAATGGTATACATA+AGG 0.511316 2:-15573500 MsG0280007445.01.T01:CDS
CCAATGCTATTGAGGGAAAG+TGG 0.511925 2:-15572122 MsG0280007445.01.T01:CDS
CGGGCAAATACTGGGTTACG+GGG 0.520947 2:+15573198 None:intergenic
GACGGGCAAATACTGGGTTA+CGG 0.523874 2:+15573196 None:intergenic
CGCAAACATAGCAAGAATCC+TGG 0.525671 2:-15576107 MsG0280007445.01.T01:CDS
TGTTCTTGGAATGCGAACAT+AGG 0.529404 2:+15573446 None:intergenic
GTGCTGCTTACTGTATTACA+AGG 0.530206 2:-15572149 MsG0280007445.01.T01:CDS
AGAGTTGACGGGCAAATACT+GGG 0.541627 2:+15573190 None:intergenic
AGAACATTCAAACGGTTCGC+TGG 0.554467 2:-15573429 MsG0280007445.01.T01:CDS
GCATTCCAAGAACATTCAAA+CGG 0.560811 2:-15573437 MsG0280007445.01.T01:CDS
AGGAGCAATGGCACATGGGA+AGG 0.566159 2:-15572608 MsG0280007445.01.T01:CDS
AAGGTAGGAGCAATGGCACA+TGG 0.567635 2:-15572613 MsG0280007445.01.T01:CDS
CTAATTTAAGGTAGGAGCAA+TGG 0.567697 2:-15572620 MsG0280007445.01.T01:intron
CCAACCTGATGACAAATGTC+AGG 0.576918 2:+15576074 None:intergenic
ATGTCAGGTCTGTAATCACC+AGG 0.611984 2:+15576089 None:intergenic
ATAGCCCGCTAAATAAAGCT+GGG 0.615108 2:-15573524 MsG0280007445.01.T01:CDS
ACATAGAGATAATTTAGACT+AGG 0.635079 2:+15571997 None:intergenic
GATTCTTGCTATGTTTGCGG+AGG 0.637726 2:+15576111 None:intergenic
AGGTAGGAGCAATGGCACAT+GGG 0.662380 2:-15572612 MsG0280007445.01.T01:CDS
TAGGGAGTACCACAAAGACA+AGG 0.712278 2:+15572702 None:intergenic
GCCCGCTAAATAAAGCTGGG+AGG 0.725844 2:-15573521 MsG0280007445.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTTACATATGAA+TGG - Chr2:15573084-15573103 MsG0280007445.01.T01:intron 10.0%
!! AAATACATTAAGATCATATT+TGG + Chr2:15575813-15575832 None:intergenic 15.0%
!! AACAAAAGAAAGATAAAATA+AGG - Chr2:15573893-15573912 MsG0280007445.01.T01:intron 15.0%
!! ACAAAAGAAAGATAAAATAA+GGG - Chr2:15573894-15573913 MsG0280007445.01.T01:intron 15.0%
!! TATGATACTATCTTAAAATT+TGG + Chr2:15572699-15572718 None:intergenic 15.0%
!!! AAATTTTAAGATAGTATCAT+AGG - Chr2:15572698-15572717 MsG0280007445.01.T01:intron 15.0%
!!! CAAATTTTTTTTTTTGCTTT+TGG - Chr2:15572505-15572524 MsG0280007445.01.T01:intron 15.0%
!!! TGATACATTTCTAAAAATTA+AGG - Chr2:15574376-15574395 MsG0280007445.01.T01:intron 15.0%
!! AACATTATATATGCTCTTAA+CGG + Chr2:15573418-15573437 None:intergenic 20.0%
!! ACTATCTTAAAATTTGGATT+AGG + Chr2:15572693-15572712 None:intergenic 20.0%
!! ATTGAACCTTTAAATCAATT+CGG + Chr2:15573782-15573801 None:intergenic 20.0%
!! GAATATTTGTAGCAATTATT+GGG - Chr2:15572454-15572473 MsG0280007445.01.T01:intron 20.0%
!! GTTCTTAATTTATTCTAAGT+GGG - Chr2:15573543-15573562 MsG0280007445.01.T01:CDS 20.0%
!! TAATCAAATGAAATAACATG+CGG + Chr2:15575154-15575173 None:intergenic 20.0%
!! TACAATTGATAAAAACAGTT+TGG + Chr2:15572013-15572032 None:intergenic 20.0%
!! TGAATATTTGTAGCAATTAT+TGG - Chr2:15572453-15572472 MsG0280007445.01.T01:intron 20.0%
!! TGTTCTTAATTTATTCTAAG+TGG - Chr2:15573542-15573561 MsG0280007445.01.T01:CDS 20.0%
!!! CTTTTGAATGATCTAATTTA+AGG - Chr2:15575427-15575446 MsG0280007445.01.T01:intron 20.0%
!!! GCAAATATTATCATATTTTG+AGG - Chr2:15574432-15574451 MsG0280007445.01.T01:intron 20.0%
!!! TTTTAGAAATGTATCAGTTT+TGG + Chr2:15574372-15574391 None:intergenic 20.0%
!!! TTTTAGCAATCTTTAATTCT+AGG - Chr2:15573015-15573034 MsG0280007445.01.T01:intron 20.0%
!!! TTTTTGTTCTTCAAACATTT+TGG - Chr2:15573447-15573466 MsG0280007445.01.T01:CDS 20.0%
! AAAATGCTTTGATACCAATT+AGG + Chr2:15576162-15576181 None:intergenic 25.0%
! AAATGCTTTGATACCAATTA+GGG + Chr2:15576161-15576180 None:intergenic 25.0%
! AAGATAAACCTAACATTCAA+AGG + Chr2:15575276-15575295 None:intergenic 25.0%
! AATCATCAACTGAGATAAAT+AGG + Chr2:15575405-15575424 None:intergenic 25.0%
! AATTGTTTCCTTTGAATGTT+AGG - Chr2:15575265-15575284 MsG0280007445.01.T01:intron 25.0%
! ACATAAAAAACAAGTTCTCA+TGG - Chr2:15573701-15573720 MsG0280007445.01.T01:intron 25.0%
! ACATAGAGATAATTTAGACT+AGG + Chr2:15576065-15576084 None:intergenic 25.0%
! ACATCTTACTTCATTATTCA+TGG + Chr2:15573151-15573170 None:intergenic 25.0%
! AGTTTATCATATTTAGCATG+AGG + Chr2:15572163-15572182 None:intergenic 25.0%
! ATTGTCTTCATACAAGTTAT+AGG + Chr2:15575710-15575729 None:intergenic 25.0%
! GAACAATAGATCAGAAAAAT+AGG + Chr2:15572947-15572966 None:intergenic 25.0%
! TGAAAGTTGAATACAAATCA+AGG + Chr2:15574148-15574167 None:intergenic 25.0%
! TGCGAATCAAATTATAGTTT+AGG - Chr2:15572218-15572237 MsG0280007445.01.T01:intron 25.0%
!! CCACATATAAACACATTTTT+GGG + Chr2:15572624-15572643 None:intergenic 25.0%
!! TCCACATATAAACACATTTT+TGG + Chr2:15572625-15572644 None:intergenic 25.0%
!! TGAATGATCTAATTTAAGGT+AGG - Chr2:15575431-15575450 MsG0280007445.01.T01:intron 25.0%
!!! ATATTACAAGACAGTTTTGT+AGG - Chr2:15572854-15572873 MsG0280007445.01.T01:intron 25.0%
!!! CATTCACTTTTCCTTAAATT+TGG - Chr2:15575521-15575540 MsG0280007445.01.T01:intron 25.0%
!!! CGATATAAAATGTTGTTTTC+AGG - Chr2:15574494-15574513 MsG0280007445.01.T01:intron 25.0%
!!! GAGTCATTTTTTAAACCATT+GGG + Chr2:15574922-15574941 None:intergenic 25.0%
!!! TTTTTTATGTTCTATCCCAT+TGG + Chr2:15573691-15573710 None:intergenic 25.0%
AAACAATATTCACGTATCCA+TGG + Chr2:15574262-15574281 None:intergenic 30.0%
AAACACAACAGTGTACAAAA+AGG + Chr2:15575222-15575241 None:intergenic 30.0%
AACTTTGTGTTCTCAATCTT+TGG + Chr2:15573828-15573847 None:intergenic 30.0%
AATAAGCTGAAAACAGCTTA+TGG + Chr2:15575653-15575672 None:intergenic 30.0%
AGAATCAGATTTCAAGGTTA+AGG + Chr2:15572278-15572297 None:intergenic 30.0%
ATAAGCTGAAAACAGCTTAT+GGG + Chr2:15575652-15575671 None:intergenic 30.0%
ATCTCTATCTTCTACATCAT+AGG - Chr2:15576125-15576144 MsG0280007445.01.T01:CDS 30.0%
ATGTCGTTGGATAGTAAAAA+GGG - Chr2:15574066-15574085 MsG0280007445.01.T01:intron 30.0%
ATGTGAAGAAAGCAAAAATC+TGG + Chr2:15573296-15573315 None:intergenic 30.0%
ATTACTTGTACTATGCACTT+TGG + Chr2:15573068-15573087 None:intergenic 30.0%
ATTTGACTGTGTTAACACTT+AGG - Chr2:15574676-15574695 MsG0280007445.01.T01:intron 30.0%
CAACATTGTACATTCTTTGA+GGG + Chr2:15572429-15572448 None:intergenic 30.0%
CAACTGAGATAAATAGGTAA+AGG + Chr2:15575399-15575418 None:intergenic 30.0%
CCCAAAAATGTGTTTATATG+TGG - Chr2:15572621-15572640 MsG0280007445.01.T01:intron 30.0%
CCTAATAGAATCAGATTTCA+AGG + Chr2:15572284-15572303 None:intergenic 30.0%
CCTTGAAATCTGATTCTATT+AGG - Chr2:15572281-15572300 MsG0280007445.01.T01:intron 30.0%
CTAATTGTGAATGTAGCATT+GGG + Chr2:15573186-15573205 None:intergenic 30.0%
CTCCAGATATACAGAATTTA+GGG - Chr2:15572766-15572785 MsG0280007445.01.T01:CDS 30.0%
CTTACTTCATTATTCATGGT+TGG + Chr2:15573147-15573166 None:intergenic 30.0%
GAATTTGAACAATTCGCTAT+TGG + Chr2:15575076-15575095 None:intergenic 30.0%
GACAATACATTAAAGCTTTG+AGG + Chr2:15572362-15572381 None:intergenic 30.0%
GGTTGAAAATGGTATACATA+AGG - Chr2:15574559-15574578 MsG0280007445.01.T01:intron 30.0%
GTATACATAAGGACTGAAAA+AGG - Chr2:15574570-15574589 MsG0280007445.01.T01:intron 30.0%
TACACAGATGATTATATAGC+TGG - Chr2:15575485-15575504 MsG0280007445.01.T01:intron 30.0%
TATATATGCTCTTAACGGTT+TGG + Chr2:15573413-15573432 None:intergenic 30.0%
TCATTTGATTAGATAGACAG+TGG - Chr2:15575163-15575182 MsG0280007445.01.T01:intron 30.0%
TGCATTAGAGTAAAACTACA+TGG + Chr2:15573509-15573528 None:intergenic 30.0%
TGTCAGAAAAAGAAGAAAAC+AGG + Chr2:15573619-15573638 None:intergenic 30.0%
TTAGCTTCTTCTATTTATGC+TGG - Chr2:15575973-15575992 MsG0280007445.01.T01:intron 30.0%
TTCAAGCTTTCACTATATTG+TGG + Chr2:15573368-15573387 None:intergenic 30.0%
TTTAAGGAAAAGTGAATGCA+AGG + Chr2:15575519-15575538 None:intergenic 30.0%
! AAAAACGAGACAAAGTTTTG+GGG - Chr2:15574401-15574420 MsG0280007445.01.T01:intron 30.0%
! ACAAAAACGAGACAAAGTTT+TGG - Chr2:15574399-15574418 MsG0280007445.01.T01:intron 30.0%
! AGAGATTATTCATTTTCCTC+AGG + Chr2:15574738-15574757 None:intergenic 30.0%
! ATTTGATTCGCAGTATTTGT+TGG + Chr2:15572210-15572229 None:intergenic 30.0%
! CAAAAACGAGACAAAGTTTT+GGG - Chr2:15574400-15574419 MsG0280007445.01.T01:intron 30.0%
! CTCAAACCGAATTGATTTAA+AGG - Chr2:15573773-15573792 MsG0280007445.01.T01:intron 30.0%
! CTTTTTCTGACAAGTTATTG+CGG - Chr2:15573627-15573646 MsG0280007445.01.T01:intron 30.0%
! TAGTTTTCGGACCAAATTTA+AGG + Chr2:15575535-15575554 None:intergenic 30.0%
! TGTTTTCTGATTTCAGTTTC+TGG - Chr2:15575876-15575895 MsG0280007445.01.T01:intron 30.0%
!! GTTGTTTTCATAAGCTATCT+TGG + Chr2:15575688-15575707 None:intergenic 30.0%
!! TGAAAAAGGTGTTCTTATAG+AGG - Chr2:15574584-15574603 MsG0280007445.01.T01:intron 30.0%
!! TGTTCTTCAAACATTTTGGT+CGG - Chr2:15573451-15573470 MsG0280007445.01.T01:CDS 30.0%
!! TTTATCACCATTTTTCGTTG+AGG - Chr2:15574178-15574197 MsG0280007445.01.T01:intron 30.0%
!! TTTGAGACACTTCTGATTTT+TGG - Chr2:15574279-15574298 MsG0280007445.01.T01:intron 30.0%
!!! GGAGTCATTTTTTAAACCAT+TGG + Chr2:15574923-15574942 None:intergenic 30.0%
!!! TATACATTGTTGTTTTGCAG+TGG - Chr2:15574771-15574790 MsG0280007445.01.T01:intron 30.0%
AAAAGAAGAAAACAGGAGAG+AGG + Chr2:15573612-15573631 None:intergenic 35.0%
AAAGAAGAAAACAGGAGAGA+GGG + Chr2:15573611-15573630 None:intergenic 35.0%
AAATTGAGTATAACTGCTGC+TGG - Chr2:15574805-15574824 MsG0280007445.01.T01:intron 35.0%
AAGGATTACCAATGCTATTG+AGG - Chr2:15575929-15575948 MsG0280007445.01.T01:intron 35.0%
AGCAGCATAAACATCAAACT+AGG + Chr2:15572055-15572074 None:intergenic 35.0%
AGGAATTCGAAAATTGAGCT+AGG + Chr2:15575379-15575398 None:intergenic 35.0%
AGGATTACCAATGCTATTGA+GGG - Chr2:15575930-15575949 MsG0280007445.01.T01:intron 35.0%
ATACAGTAAGCAGCACTTAA+AGG + Chr2:15575906-15575925 None:intergenic 35.0%
ATCCATAAACTGAACCAAAC+TGG + Chr2:15574306-15574325 None:intergenic 35.0%
ATTGCTAAAAAGCCCTTTGA+GGG + Chr2:15573005-15573024 None:intergenic 35.0%
CAACAATTGACAACACTGAA+TGG + Chr2:15574944-15574963 None:intergenic 35.0%
CACCCTAAATTCTGTATATC+TGG + Chr2:15572771-15572790 None:intergenic 35.0%
CATGTCGTTGGATAGTAAAA+AGG - Chr2:15574065-15574084 MsG0280007445.01.T01:intron 35.0%
CCAACATTGTACATTCTTTG+AGG + Chr2:15572430-15572449 None:intergenic 35.0%
CCTCAAAGAATGTACAATGT+TGG - Chr2:15572427-15572446 MsG0280007445.01.T01:intron 35.0%
CTCCTACTTATAACAGTTTC+AGG + Chr2:15572825-15572844 None:intergenic 35.0%
CTTAATGTATTTGTGCACGA+TGG - Chr2:15575821-15575840 MsG0280007445.01.T01:intron 35.0%
CTTCTTCTATTTATGCTGGA+TGG - Chr2:15575977-15575996 MsG0280007445.01.T01:intron 35.0%
GAAAGCTTGAATCATCACTT+CGG - Chr2:15573377-15573396 MsG0280007445.01.T01:intron 35.0%
GATCGCAGAAAATAACAGTT+CGG - Chr2:15574963-15574982 MsG0280007445.01.T01:intron 35.0%
GATTAGATAGACAGTGGAAT+TGG - Chr2:15575169-15575188 MsG0280007445.01.T01:intron 35.0%
GCATTCCAAGAACATTCAAA+CGG - Chr2:15574622-15574641 MsG0280007445.01.T01:intron 35.0%
GCTAATTGTGAATGTAGCAT+TGG + Chr2:15573187-15573206 None:intergenic 35.0%
GCTCCAGATATACAGAATTT+AGG - Chr2:15572765-15572784 MsG0280007445.01.T01:CDS 35.0%
GGAATTCGAAAATTGAGCTA+GGG + Chr2:15575378-15575397 None:intergenic 35.0%
TAAAAGGACAAAGTATGTCC+TGG - Chr2:15573234-15573253 MsG0280007445.01.T01:CDS 35.0%
TAAGCATTTGTGAGACTGTT+TGG - Chr2:15575598-15575617 MsG0280007445.01.T01:intron 35.0%
TACAGTAAGCAGCACTTAAA+GGG + Chr2:15575905-15575924 None:intergenic 35.0%
TATGTTCTATCCCATTGGAA+TGG + Chr2:15573686-15573705 None:intergenic 35.0%
TCAAATTAGCCAATCCAAAC+AGG + Chr2:15575563-15575582 None:intergenic 35.0%
TCATAACCGAGTCAAAAACT+TGG + Chr2:15572535-15572554 None:intergenic 35.0%
TGACAACACTGATATCATGA+AGG - Chr2:15575128-15575147 MsG0280007445.01.T01:intron 35.0%
TTTAGCACCTCAACGAAAAA+TGG + Chr2:15574188-15574207 None:intergenic 35.0%
! ACAGAATTTAGGGTGTGTTT+AGG - Chr2:15572776-15572795 MsG0280007445.01.T01:intron 35.0%
! CAGAATTTAGGGTGTGTTTA+GGG - Chr2:15572777-15572796 MsG0280007445.01.T01:intron 35.0%
! CTAATTTAAGGTAGGAGCAA+TGG - Chr2:15575439-15575458 MsG0280007445.01.T01:intron 35.0%
! TAGTTATTGTTGTTGCCCAA+TGG - Chr2:15574904-15574923 MsG0280007445.01.T01:intron 35.0%
! TGCATGATTTTCTTTTCCTG+AGG - Chr2:15574719-15574738 MsG0280007445.01.T01:intron 35.0%
! TGTTCTATGCTTTGTTCTTG+AGG + Chr2:15573727-15573746 None:intergenic 35.0%
!! AAAACGAGACAAAGTTTTGG+GGG - Chr2:15574402-15574421 MsG0280007445.01.T01:intron 35.0%
!! CAAACAGGCTTCTTAGTTTT+CGG + Chr2:15575548-15575567 None:intergenic 35.0%
!! TGACTTTGTTGGGTTGATTA+AGG - Chr2:15572667-15572686 MsG0280007445.01.T01:intron 35.0%
!! TGCAAGGTGTTGTTAAATAG+TGG + Chr2:15575035-15575054 None:intergenic 35.0%
!!! ATCTTTTAGTTCAGAGAAGC+TGG - Chr2:15575291-15575310 MsG0280007445.01.T01:intron 35.0%
!!! TCCTTTTAAAGCAAGCTAAC+CGG + Chr2:15573222-15573241 None:intergenic 35.0%
!!! TTCTGATTTTTGGTCCAGTT+TGG - Chr2:15574289-15574308 MsG0280007445.01.T01:intron 35.0%
AACAAAGCATAGAACAGCGT+TGG - Chr2:15573731-15573750 MsG0280007445.01.T01:intron 40.0%
AACAAAGTCAGCTTGTAGGT+TGG + Chr2:15572657-15572676 None:intergenic 40.0%
AAGAAGAAAACAGGAGAGAG+GGG + Chr2:15573610-15573629 None:intergenic 40.0%
AATTAGCCAACTATCAGTGC+CGG - Chr2:15573200-15573219 MsG0280007445.01.T01:CDS 40.0%
ACAAAGTCAGCTTGTAGGTT+GGG + Chr2:15572656-15572675 None:intergenic 40.0%
ACATATGAATGGCAGTGAAG+AGG - Chr2:15573095-15573114 MsG0280007445.01.T01:intron 40.0%
ACCTTGCACTAATAGTTGGT+GGG - Chr2:15575047-15575066 MsG0280007445.01.T01:intron 40.0%
ACGTTGCTAACGAATCTACT+TGG - Chr2:15572133-15572152 MsG0280007445.01.T01:CDS 40.0%
ACTAAGAAGCCTGTTTGGAT+TGG - Chr2:15575551-15575570 MsG0280007445.01.T01:intron 40.0%
AGCACTGTGTATACTATTGC+TGG - Chr2:15572974-15572993 MsG0280007445.01.T01:intron 40.0%
AGGAACTCTTGTTCCCTAAT+TGG - Chr2:15576145-15576164 MsG0280007445.01.T01:CDS 40.0%
ATAGCCCGCTAAATAAAGCT+GGG - Chr2:15574535-15574554 MsG0280007445.01.T01:intron 40.0%
ATAGGCTATCCAAGATTCGT+TGG - Chr2:15572716-15572735 MsG0280007445.01.T01:CDS 40.0%
CAACACCTTGCACTAATAGT+TGG - Chr2:15575043-15575062 MsG0280007445.01.T01:intron 40.0%
CCATGTCATTCCATTCCAAT+GGG - Chr2:15573673-15573692 MsG0280007445.01.T01:intron 40.0%
CGAAAACTAAGAAGCCTGTT+TGG - Chr2:15575546-15575565 MsG0280007445.01.T01:intron 40.0%
CTACTGACCAATCAATGTCA+GGG - Chr2:15573041-15573060 MsG0280007445.01.T01:intron 40.0%
GATTGCTAAAAAGCCCTTTG+AGG + Chr2:15573006-15573025 None:intergenic 40.0%
GCTTAGCTTAGCTAAATGTG+AGG + Chr2:15572319-15572338 None:intergenic 40.0%
GGCAGTGTGTATGACTTTAA+AGG - Chr2:15574228-15574247 MsG0280007445.01.T01:intron 40.0%
GGCCTGAAACTGTTATAAGT+AGG - Chr2:15572820-15572839 MsG0280007445.01.T01:intron 40.0%
GTCAACAATTCCAAGCAATC+AGG - Chr2:15575333-15575352 MsG0280007445.01.T01:intron 40.0%
GTTCTTGGAATGCGAACATA+GGG + Chr2:15574615-15574634 None:intergenic 40.0%
TAAAGCTGGGAGGTTGAAAA+TGG - Chr2:15574548-15574567 MsG0280007445.01.T01:intron 40.0%
TAACCGAGTCAAAAACTTGG+TGG + Chr2:15572532-15572551 None:intergenic 40.0%
TAGGCTATCCAAGATTCGTT+GGG - Chr2:15572717-15572736 MsG0280007445.01.T01:CDS 40.0%
TCCATGTCATTCCATTCCAA+TGG - Chr2:15573672-15573691 MsG0280007445.01.T01:intron 40.0%
TGTTCTTGGAATGCGAACAT+AGG + Chr2:15574616-15574635 None:intergenic 40.0%
TTATGCTGCTAGATACCACT+AGG - Chr2:15572065-15572084 MsG0280007445.01.T01:three_prime_UTR 40.0%
! AACCTACAAGCTGACTTTGT+TGG - Chr2:15572656-15572675 MsG0280007445.01.T01:intron 40.0%
! ACCTACAAGCTGACTTTGTT+GGG - Chr2:15572657-15572676 MsG0280007445.01.T01:intron 40.0%
! CAGGATTCTTGCTATGTTTG+CGG + Chr2:15571954-15571973 None:intergenic 40.0%
! CTTTAAAGGCTTGAAGACCA+TGG - Chr2:15574242-15574261 MsG0280007445.01.T01:intron 40.0%
! GTCCAGTTTGGTTCAGTTTA+TGG - Chr2:15574301-15574320 MsG0280007445.01.T01:intron 40.0%
! TAGTGTTTTCCCGATTCAAG+CGG + Chr2:15572101-15572120 None:intergenic 40.0%
! TGTGAGGTGCTTCAATATAG+AGG - Chr2:15573976-15573995 MsG0280007445.01.T01:intron 40.0%
! TGTTGGTTAATGCAGTCGAA+TGG + Chr2:15572193-15572212 None:intergenic 40.0%
!! AACCTCCCAGCTTTATTTAG+CGG + Chr2:15574543-15574562 None:intergenic 40.0%
!! GTGCTGCTTACTGTATTACA+AGG - Chr2:15575910-15575929 MsG0280007445.01.T01:intron 40.0%
!!! AGTTTTGGTCTTGTAGCTAG+GGG + Chr2:15574357-15574376 None:intergenic 40.0%
!!! CAGTTTTGGTCTTGTAGCTA+GGG + Chr2:15574358-15574377 None:intergenic 40.0%
!!! TCAGTTTTGGTCTTGTAGCT+AGG + Chr2:15574359-15574378 None:intergenic 40.0%
AACGACATGTCTCGATCAGA+CGG + Chr2:15574054-15574073 None:intergenic 45.0%
AAGACAAGGTCCTGATTGCT+TGG + Chr2:15575346-15575365 None:intergenic 45.0%
AAGCACCTCACACTTCTCTT+TGG + Chr2:15573968-15573987 None:intergenic 45.0%
AAGCTATCTTGGAGAGCTTG+AGG + Chr2:15575677-15575696 None:intergenic 45.0%
AATATAGAGGCCACGACGAT+GGG - Chr2:15573989-15574008 MsG0280007445.01.T01:intron 45.0%
ACCCAACAAAGTCAGCTTGT+AGG + Chr2:15572661-15572680 None:intergenic 45.0%
AGAACATTCAAACGGTTCGC+TGG - Chr2:15574630-15574649 MsG0280007445.01.T01:intron 45.0%
AGCACCTCACACTTCTCTTT+GGG + Chr2:15573967-15573986 None:intergenic 45.0%
ATAGCAAGCCCAACGAATCT+TGG + Chr2:15572728-15572747 None:intergenic 45.0%
ATAGCAGCGCTATAGCAAAG+CGG + Chr2:15575098-15575117 None:intergenic 45.0%
ATGGAATGACATGGAAGCCA+CGG + Chr2:15573667-15573686 None:intergenic 45.0%
ATGTCAGGTCTGTAATCACC+AGG + Chr2:15571973-15571992 None:intergenic 45.0%
CAAAGTCAGCTTGTAGGTTG+GGG + Chr2:15572655-15572674 None:intergenic 45.0%
CAATCAACATGAGACATGCC+AGG + Chr2:15573255-15573274 None:intergenic 45.0%
CAATCAGGACCTTGTCTTTG+TGG - Chr2:15575348-15575367 MsG0280007445.01.T01:intron 45.0%
CACCTTGCACTAATAGTTGG+TGG - Chr2:15575046-15575065 MsG0280007445.01.T01:intron 45.0%
CAGACCTGACATTTGTCATC+AGG - Chr2:15571981-15572000 MsG0280007445.01.T01:three_prime_UTR 45.0%
CCAACCTGATGACAAATGTC+AGG + Chr2:15571988-15572007 None:intergenic 45.0%
CCAATGCTATTGAGGGAAAG+TGG - Chr2:15575937-15575956 MsG0280007445.01.T01:intron 45.0%
CCACTTTCCCTCAATAGCAT+TGG + Chr2:15575940-15575959 None:intergenic 45.0%
CCCATTGGAATGGAATGACA+TGG + Chr2:15573676-15573695 None:intergenic 45.0%
CCTGACATTTGTCATCAGGT+TGG - Chr2:15571985-15572004 MsG0280007445.01.T01:three_prime_UTR 45.0%
CGCAAACATAGCAAGAATCC+TGG - Chr2:15571952-15571971 MsG0280007445.01.T01:three_prime_UTR 45.0%
CTTTGGACCCTGACATTGAT+TGG + Chr2:15573051-15573070 None:intergenic 45.0%
GAATCCACATGTGGCAATGA+TGG + Chr2:15573478-15573497 None:intergenic 45.0%
GATAGCCCGCTAAATAAAGC+TGG - Chr2:15574534-15574553 MsG0280007445.01.T01:intron 45.0%
GCGAACCGTTTGAATGTTCT+TGG + Chr2:15574630-15574649 None:intergenic 45.0%
GCTACTGACCAATCAATGTC+AGG - Chr2:15573040-15573059 MsG0280007445.01.T01:intron 45.0%
GCTGTTCTTGAATCCACATG+TGG + Chr2:15573487-15573506 None:intergenic 45.0%
GTGCTAAAACTCTAGGTTGC+TGG - Chr2:15574200-15574219 MsG0280007445.01.T01:intron 45.0%
TATTCTAAGTGGGTCCCACA+CGG - Chr2:15573553-15573572 MsG0280007445.01.T01:intron 45.0%
TCCCACCAACTATTAGTGCA+AGG + Chr2:15575051-15575070 None:intergenic 45.0%
! AAAGTCAGCTTGTAGGTTGG+GGG + Chr2:15572654-15572673 None:intergenic 45.0%
! AGAGTTGACGGGCAAATACT+GGG + Chr2:15574872-15574891 None:intergenic 45.0%
! AGCGTTGGAGATGCTCTAAT+AGG - Chr2:15573746-15573765 MsG0280007445.01.T01:intron 45.0%
! AGTGTTTTCCCGATTCAAGC+GGG + Chr2:15572100-15572119 None:intergenic 45.0%
! CGTTGAGGTGCTAAAACTCT+AGG - Chr2:15574193-15574212 MsG0280007445.01.T01:intron 45.0%
! GATTCTTGCTATGTTTGCGG+AGG + Chr2:15571951-15571970 None:intergenic 45.0%
! GTACATTCTTTGAGGGAGAG+AGG + Chr2:15572422-15572441 None:intergenic 45.0%
! TTACGGGGTTTTTGATCGTG+CGG + Chr2:15574849-15574868 None:intergenic 45.0%
! TTTAGGGTCCCACATTGGAT+GGG - Chr2:15572793-15572812 MsG0280007445.01.T01:intron 45.0%
!! AACTCTAGGTTGCTGGTTCA+AGG - Chr2:15574207-15574226 MsG0280007445.01.T01:intron 45.0%
!! ACCTCCCAGCTTTATTTAGC+GGG + Chr2:15574542-15574561 None:intergenic 45.0%
!! ACCTGCTTTTCGAGAGTTGA+CGG + Chr2:15574884-15574903 None:intergenic 45.0%
!! GATTCGTTGGGCTTGCTATT+AGG - Chr2:15572729-15572748 MsG0280007445.01.T01:CDS 45.0%
!! GCCGGTTAGCTTGCTTTAAA+AGG - Chr2:15573218-15573237 MsG0280007445.01.T01:CDS 45.0%
!! GGACCACCAAGTTTTTGACT+CGG - Chr2:15572526-15572545 MsG0280007445.01.T01:intron 45.0%
!! TAGGGAGTACCACAAAGACA+AGG + Chr2:15575360-15575379 None:intergenic 45.0%
AAAACAGGAGAGAGGGGAAG+TGG + Chr2:15573604-15573623 None:intergenic 50.0%
AAACAGGAGAGAGGGGAAGT+GGG + Chr2:15573603-15573622 None:intergenic 50.0%
AAGGTAGGAGCAATGGCACA+TGG - Chr2:15575446-15575465 MsG0280007445.01.T01:intron 50.0%
ACGACATGTCTCGATCAGAC+GGG + Chr2:15574053-15574072 None:intergenic 50.0%
ACGGGCAAATACTGGGTTAC+GGG + Chr2:15574865-15574884 None:intergenic 50.0%
AGAACAGACCCCGCTTGAAT+CGG - Chr2:15572088-15572107 MsG0280007445.01.T01:three_prime_UTR 50.0%
AGAGAGGAACCCTTCCAATC+AGG + Chr2:15572406-15572425 None:intergenic 50.0%
AGGCCATATCCCATCCAATG+TGG + Chr2:15572805-15572824 None:intergenic 50.0%
AGGTAGGAGCAATGGCACAT+GGG - Chr2:15575447-15575466 MsG0280007445.01.T01:intron 50.0%
ATATAGAGGCCACGACGATG+GGG - Chr2:15573990-15574009 MsG0280007445.01.T01:intron 50.0%
ATGCCTTTAGTCGTCAGCCT+CGG + Chr2:15574026-15574045 None:intergenic 50.0%
ATTGCTGGCATCTCCCTCAA+AGG - Chr2:15572989-15573008 MsG0280007445.01.T01:intron 50.0%
CAATATAGAGGCCACGACGA+TGG - Chr2:15573988-15574007 MsG0280007445.01.T01:intron 50.0%
CCTCACACTTCTCTTTGGGT+TGG + Chr2:15573963-15573982 None:intergenic 50.0%
CTACCATGGCCTGATTGGAA+GGG - Chr2:15572394-15572413 MsG0280007445.01.T01:intron 50.0%
GACGGGCAAATACTGGGTTA+CGG + Chr2:15574866-15574885 None:intergenic 50.0%
GGCCATATCCCATCCAATGT+GGG + Chr2:15572804-15572823 None:intergenic 50.0%
GTCCCACATTGGATGGGATA+TGG - Chr2:15572799-15572818 MsG0280007445.01.T01:intron 50.0%
GTCTGATCGAGACATGTCGT+TGG - Chr2:15574053-15574072 MsG0280007445.01.T01:intron 50.0%
TCTTGTTCCACAGCCTACCA+TGG - Chr2:15572380-15572399 MsG0280007445.01.T01:intron 50.0%
TGGAATGACATGGAAGCCAC+GGG + Chr2:15573666-15573685 None:intergenic 50.0%
TTGCTGGCATCTCCCTCAAA+GGG - Chr2:15572990-15573009 MsG0280007445.01.T01:intron 50.0%
! CCAACCCAAAGAGAAGTGTG+AGG - Chr2:15573960-15573979 MsG0280007445.01.T01:intron 50.0%
! GAGAGTTGACGGGCAAATAC+TGG + Chr2:15574873-15574892 None:intergenic 50.0%
! GCTAGGGGTGTGCAAAAATG+TGG + Chr2:15574342-15574361 None:intergenic 50.0%
! GGAAGCGCGCTTAATTACTG+TGG + Chr2:15572341-15572360 None:intergenic 50.0%
! GTGTGTTTAGGGTCCCACAT+TGG - Chr2:15572788-15572807 MsG0280007445.01.T01:intron 50.0%
! GTGTTTTCCCGATTCAAGCG+GGG + Chr2:15572099-15572118 None:intergenic 50.0%
! GTTTAGGGTCCCACATTGGA+TGG - Chr2:15572792-15572811 MsG0280007445.01.T01:intron 50.0%
!! AGCTAACCGGCACTGATAGT+TGG + Chr2:15573209-15573228 None:intergenic 50.0%
!! CACACTTCTCTTTGGGTTGG+TGG + Chr2:15573960-15573979 None:intergenic 50.0%
!! CCTGCTTTTCGAGAGTTGAC+GGG + Chr2:15574883-15574902 None:intergenic 50.0%
AATCAGGCCATGGTAGGCTG+TGG + Chr2:15572390-15572409 None:intergenic 55.0%
ACAGCCTACCATGGCCTGAT+TGG - Chr2:15572389-15572408 MsG0280007445.01.T01:intron 55.0%
AGGAGCAATGGCACATGGGA+AGG - Chr2:15575451-15575470 MsG0280007445.01.T01:intron 55.0%
CCCGTCAACTCTCGAAAAGC+AGG - Chr2:15574880-15574899 MsG0280007445.01.T01:intron 55.0%
CCTACCATGGCCTGATTGGA+AGG - Chr2:15572393-15572412 MsG0280007445.01.T01:intron 55.0%
CCTTCCAATCAGGCCATGGT+AGG + Chr2:15572396-15572415 None:intergenic 55.0%
CGACATGTCTCGATCAGACG+GGG + Chr2:15574052-15574071 None:intergenic 55.0%
CGGAAAACAGTGTTCTCCCG+TGG - Chr2:15573647-15573666 MsG0280007445.01.T01:intron 55.0%
CGGGCAAATACTGGGTTACG+GGG + Chr2:15574864-15574883 None:intergenic 55.0%
CGGTCCATCATTGCCACATG+TGG - Chr2:15573471-15573490 MsG0280007445.01.T01:CDS 55.0%
GAACAGACCCCGCTTGAATC+GGG - Chr2:15572089-15572108 MsG0280007445.01.T01:three_prime_UTR 55.0%
GAACCCTTCCAATCAGGCCA+TGG + Chr2:15572400-15572419 None:intergenic 55.0%
GCCCGCTAAATAAAGCTGGG+AGG - Chr2:15574538-15574557 MsG0280007445.01.T01:intron 55.0%
TATAGAGGCCACGACGATGG+GGG - Chr2:15573991-15574010 MsG0280007445.01.T01:intron 55.0%
TGTCCGAGGCTGACGACTAA+AGG - Chr2:15574020-15574039 MsG0280007445.01.T01:intron 55.0%
TTAGTCGTCAGCCTCGGACA+CGG + Chr2:15574020-15574039 None:intergenic 55.0%
! TATCTGGAGCATGAGCCGAG+TGG + Chr2:15572755-15572774 None:intergenic 55.0%
!! GGGCTTGCTATTAGGCCACT+CGG - Chr2:15572737-15572756 MsG0280007445.01.T01:CDS 55.0%
AGCGGGGTCTGTTCTCCTAG+TGG + Chr2:15572083-15572102 None:intergenic 60.0%
AGGAGAGAGGGGAAGTGGGT+GGG + Chr2:15573599-15573618 None:intergenic 60.0%
CGACAGTGCCAGTGAAGGAG+AGG - Chr2:15573934-15573953 MsG0280007445.01.T01:intron 60.0%
GCTGGGGAAGGAAACCGTGT+GGG + Chr2:15573570-15573589 None:intergenic 60.0%
GTGTGCGACAGTGCCAGTGA+AGG - Chr2:15573929-15573948 MsG0280007445.01.T01:intron 60.0%
!! GTTGGTGGCCTCTCCTTCAC+TGG + Chr2:15573945-15573964 None:intergenic 60.0%
CAGGAGAGAGGGGAAGTGGG+TGG + Chr2:15573600-15573619 None:intergenic 65.0%
GGAGAGAGGGGAAGTGGGTG+GGG + Chr2:15573598-15573617 None:intergenic 65.0%
GGCTGGGGAAGGAAACCGTG+TGG + Chr2:15573571-15573590 None:intergenic 65.0%
AAGTGGGTGGGGCTGCGGCT+GGG + Chr2:15573587-15573606 None:intergenic 70.0%
AGAGGCCACGACGATGGGGG+AGG - Chr2:15573994-15574013 MsG0280007445.01.T01:intron 70.0%
AGGGGAAGTGGGTGGGGCTG+CGG + Chr2:15573592-15573611 None:intergenic 70.0%
AGTGGGTGGGGCTGCGGCTG+GGG + Chr2:15573586-15573605 None:intergenic 75.0%
CACGGCCTCCCCCATCGTCG+TGG + Chr2:15574002-15574021 None:intergenic 75.0%
GAAGTGGGTGGGGCTGCGGC+TGG + Chr2:15573588-15573607 None:intergenic 75.0%
GATGGGGGAGGCCGTGTCCG+AGG - Chr2:15574006-15574025 MsG0280007445.01.T01:intron 75.0%
! GGTGGGGCTGCGGCTGGGGA+AGG + Chr2:15573582-15573601 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr2 gene 15571898 15576183 15571898 ID=MsG0280007445.01;Name=MsG0280007445.01
Chr2 mRNA 15571898 15576183 15571898 ID=MsG0280007445.01.T01;Parent=MsG0280007445.01;Name=MsG0280007445.01.T01;_AED=0.45;_eAED=0.46;_QI=0|1|0.83|1|1|1|6|212|193
Chr2 exon 15571898 15572189 15571898 ID=MsG0280007445.01.T01:exon:1079;Parent=MsG0280007445.01.T01
Chr2 exon 15572575 15572632 15572575 ID=MsG0280007445.01.T01:exon:1078;Parent=MsG0280007445.01.T01
Chr2 exon 15572712 15572794 15572712 ID=MsG0280007445.01.T01:exon:1077;Parent=MsG0280007445.01.T01
Chr2 exon 15573180 15573290 15573180 ID=MsG0280007445.01.T01:exon:1076;Parent=MsG0280007445.01.T01
Chr2 exon 15573421 15573565 15573421 ID=MsG0280007445.01.T01:exon:1075;Parent=MsG0280007445.01.T01
Chr2 exon 15576079 15576183 15576079 ID=MsG0280007445.01.T01:exon:1074;Parent=MsG0280007445.01.T01
Chr2 CDS 15576079 15576183 15576079 ID=MsG0280007445.01.T01:cds;Parent=MsG0280007445.01.T01
Chr2 CDS 15573421 15573565 15573421 ID=MsG0280007445.01.T01:cds;Parent=MsG0280007445.01.T01
Chr2 CDS 15573180 15573290 15573180 ID=MsG0280007445.01.T01:cds;Parent=MsG0280007445.01.T01
Chr2 CDS 15572712 15572794 15572712 ID=MsG0280007445.01.T01:cds;Parent=MsG0280007445.01.T01
Chr2 CDS 15572575 15572632 15572575 ID=MsG0280007445.01.T01:cds;Parent=MsG0280007445.01.T01
Chr2 CDS 15572110 15572189 15572110 ID=MsG0280007445.01.T01:cds;Parent=MsG0280007445.01.T01
Chr2 three_prime_UTR 15571898 15572109 15571898 ID=MsG0280007445.01.T01:three_prime_utr;Parent=MsG0280007445.01.T01
Gene Sequence

>MsG0280007445.01.T01

CTGTGTCTACAGACATATCAGCTGTTGAACTCTGATGAGCATGCTAATTTCCTCCGCAAACATAGCAAGAATCCTGGTGATTACAGACCTGACATTTGTCATCAGGCACTTCTATCTATTTTAGATAGCCCGCTAAATAAAGCTGGGAGGTTGAAAATGGTATACATAAGGACTGAAAAAGGTGTTCTTATAGAGGTTAAGCCCTATGTTCGCATTCCAAGAACATTCAAACGGTTCGCTGGTGTCATGTTGGAACTGCTTCAGAAATTGAGTATAACTGCTGCTGGAAAACGTGAGAAACTTCTCCGCACGATCAAAAACCCCGTAACCCAGTATTTGCCCGTCAACTCTCGAAAAGCAGGTTTATCTTTTAGTTCAGAGAAGCTGGTAGACATGAACAACTATTTGTCAACAATTCCAAGCAATCAGGACCTTGTCTTTGTGGTAGGAGCAATGGCACATGGGAAGGTTGAGACAGATTACACAGATGATTATATAGCTGTTTCTGGTTACCCTTTAAGTGCTGCTTACTGTATTACAAGGATTACCAATGCTATTGAGGGAAAGTGGAACATATTGTGA
Protein sequence

>MsG0280007445.01.T01

LCLQTYQLLNSDEHANFLRKHSKNPGDYRPDICHQALLSILDSPLNKAGRLKMVYIRTEKGVLIEVKPYVRIPRTFKRFAGVMLELLQKLSITAAGKREKLLRTIKNPVTQYLPVNSRKAGLSFSSEKLVDMNNYLSTIPSNQDLVFVVGAMAHGKVETDYTDDYIAVSGYPLSAAYCITRITNAIEGKWNIL*