AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028957.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028957.01.T01 MTR_5g078780 85.337 416 23 2 1 416 1 378 0.0 649
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028957.01.T01 AT2G17250 52.323 409 154 5 12 416 8 379 3.80e-140 412

Find 89 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATTATTGTTACGTTTCT+TGG 0.096967 5:-89577029 None:intergenic
CCACGTGAGGTAAATGAATT+TGG 0.192695 5:-89574973 None:intergenic
CTTCATCTTCTTCGCAATTC+TGG 0.241906 5:-89577265 None:intergenic
TTGGAGGGAAAAGATGTTTC+AGG 0.244460 5:+89575894 MsG0580028957.01.T01:CDS
AGAAAAGCTCACAAGAAATT+TGG 0.246404 5:+89575875 MsG0580028957.01.T01:CDS
AAGCTTGGATTGCGTATCTT+AGG 0.300738 5:+89577301 MsG0580028957.01.T01:CDS
CCAATCCATGCTGGGTCATT+AGG 0.303403 5:-89578496 None:intergenic
ATTAGTTCCATCTATCTTCA+TGG 0.328121 5:+89578553 MsG0580028957.01.T01:CDS
TGAGCTTAATTGACTTTCAT+TGG 0.332356 5:-89576083 None:intergenic
TGAATGCGATGAAACTATTA+AGG 0.339514 5:+89575052 MsG0580028957.01.T01:CDS
GAAGGGAGTTGAGGGAGGAG+AGG 0.348058 5:-89574906 None:intergenic
GTGGTGGTTGCGGTGGCGGA+AGG 0.353194 5:-89574924 None:intergenic
ATGTGGTGAGGTGGAGAAGA+AGG 0.362280 5:-89574846 None:intergenic
GCTGGTGGTGGTTGCGGTGG+CGG 0.371522 5:-89574928 None:intergenic
TCAGTGATGCTGTGTTGTTT+TGG 0.385668 5:-89574744 None:intergenic
GCTGGGTCATTAGGATATAA+AGG 0.391038 5:-89578487 None:intergenic
TGTGGTGAGGTGGAGAAGAA+GGG 0.400686 5:-89574845 None:intergenic
GAGTTTGTTAAGGTTGCTAA+TGG 0.401292 5:+89575098 MsG0580028957.01.T01:CDS
GAGATGAATGTGAGGAGGAG+AGG 0.410717 5:-89574822 None:intergenic
AGATGAATGTGAGGAGGAGA+GGG 0.412733 5:-89574821 None:intergenic
AAGGGAGTTGAGGGAGGAGA+GGG 0.413318 5:-89574905 None:intergenic
TTAGAAATGTGGAGTGTATC+AGG 0.418848 5:+89576528 MsG0580028957.01.T01:CDS
GGAGGAGCTGGTGGTGGTTG+CGG 0.422441 5:-89574934 None:intergenic
TCTTAGGTTGCCACTTCCAT+TGG 0.431065 5:+89577317 MsG0580028957.01.T01:CDS
TACTTTGATGGAGTTTGTTA+AGG 0.432512 5:+89575088 MsG0580028957.01.T01:CDS
AGTTGTGCTTGATACTTTGA+TGG 0.436883 5:+89575076 MsG0580028957.01.T01:CDS
TGTCTAATCCCATCATGTTA+TGG 0.440426 5:+89578324 MsG0580028957.01.T01:CDS
AGGGAGGAGAGGGAGGAAGA+AGG 0.440748 5:-89574895 None:intergenic
GGAGTTGAGGGAGGAGAGGG+AGG 0.440858 5:-89574902 None:intergenic
AGTTAAAATTTACTAAAGCT+TGG 0.442017 5:+89577286 MsG0580028957.01.T01:CDS
TGGTGGTTGCGGTGGCGGAA+GGG 0.462845 5:-89574923 None:intergenic
CGGTGGCGGAAGGGAGTTGA+GGG 0.464726 5:-89574914 None:intergenic
ACCACCACCAGCTCCTCCTC+TGG 0.470797 5:+89574939 MsG0580028957.01.T01:CDS
TTTCTTCATTGCAGTTTCCA+TGG 0.473343 5:-89574697 None:intergenic
TTGGGTATTCCAATCCATGC+TGG 0.473646 5:-89578505 None:intergenic
GTAAATGAATTTGGATTGAT+CGG 0.478506 5:-89574964 None:intergenic
CCTAATGACCCAGCATGGAT+TGG 0.485238 5:+89578496 MsG0580028957.01.T01:CDS
ATTTCAAGTATATTGATGTT+CGG 0.489524 5:+89575336 MsG0580028957.01.T01:CDS
GAAGAGAGAGAATCGATTCG+AGG 0.495834 5:-89574869 None:intergenic
GGAGAGAAGTTCTTGACCTA+AGG 0.496473 5:-89574775 None:intergenic
ATTGTTGATGTGTGAGGCGG+AGG 0.498436 5:-89574796 None:intergenic
GTGGTGAGGTGGAGAAGAAG+GGG 0.502021 5:-89574844 None:intergenic
ATCACTGAAATCAAAACCTT+AGG 0.502878 5:+89574759 MsG0580028957.01.T01:CDS
TTGACAAGGTCGTATGATGT+AGG 0.506754 5:+89578434 MsG0580028957.01.T01:CDS
ATCGGAACCAGAGGAGGAGC+TGG 0.519725 5:-89574946 None:intergenic
GGAGCTGGTGGTGGTTGCGG+TGG 0.521533 5:-89574931 None:intergenic
TGGGTATTCCAATCCATGCT+GGG 0.522547 5:-89578504 None:intergenic
AATAATAACTTACAATACTA+CGG 0.526362 5:-89575157 None:intergenic
TTTGTTAAGGTTGCTAATGG+TGG 0.527835 5:+89575101 MsG0580028957.01.T01:CDS
AGAGAATCGATTCGAGGATG+TGG 0.533005 5:-89574863 None:intergenic
ACCAGAGGAGGAGCTGGTGG+TGG 0.535853 5:-89574940 None:intergenic
AAGAAGGGGAGATGAATGTG+AGG 0.536341 5:-89574830 None:intergenic
CCAAATTCATTTACCTCACG+TGG 0.537243 5:+89574973 MsG0580028957.01.T01:CDS
GCGGTGGCGGAAGGGAGTTG+AGG 0.538128 5:-89574915 None:intergenic
GATAAAACTGCAAGCGCAGA+TGG 0.542599 5:+89576058 MsG0580028957.01.T01:CDS
ATCACTTATCGATTGTCTTG+TGG 0.543229 5:+89575025 MsG0580028957.01.T01:CDS
ATGAAAGTCAATTAAGCTCA+AGG 0.545065 5:+89576086 MsG0580028957.01.T01:CDS
GAGGAATCACTGCTTGGTGA+AGG 0.547446 5:-89578298 None:intergenic
ACCTCTTTGAACACATCCAA+TGG 0.549353 5:-89577333 None:intergenic
AAAGCTCACAAGAAATTTGG+AGG 0.555352 5:+89575878 MsG0580028957.01.T01:CDS
TTGAACACATCCAATGGAAG+TGG 0.555934 5:-89577327 None:intergenic
AGGTGTTTACCATAACATGA+TGG 0.563701 5:-89578333 None:intergenic
TCCATTGGATGTGTTCAAAG+AGG 0.564996 5:+89577332 MsG0580028957.01.T01:CDS
GGTGTTTACCATAACATGAT+GGG 0.565453 5:-89578332 None:intergenic
ATATGGTGTAGTACATATTG+TGG 0.566500 5:-89576464 None:intergenic
AAGGGGAGATGAATGTGAGG+AGG 0.575380 5:-89574827 None:intergenic
TGGCGGAAGGGAGTTGAGGG+AGG 0.579360 5:-89574911 None:intergenic
ACAGATGAGGAATCACTGCT+TGG 0.595439 5:-89578304 None:intergenic
GATTCGAGGATGTGGTGAGG+TGG 0.595441 5:-89574855 None:intergenic
TCATGGCAAAACATCGTGCA+AGG 0.595702 5:+89578570 MsG0580028957.01.T01:CDS
TATATCCTAATGACCCAGCA+TGG 0.606697 5:+89578491 MsG0580028957.01.T01:CDS
ACTTATCGATTGTCTTGTGG+CGG 0.611390 5:+89575028 MsG0580028957.01.T01:CDS
TTATAGCATATTGTATGACA+TGG 0.611395 5:-89576240 None:intergenic
TTTGGATTGATCGGAACCAG+AGG 0.619745 5:-89574955 None:intergenic
CATATTGTGGATGATGCACT+CGG 0.624795 5:-89576451 None:intergenic
ATACATCTGATTTAGAAATG+TGG 0.629117 5:+89576517 MsG0580028957.01.T01:CDS
AGGGAGATTGTTGATGTGTG+AGG 0.631552 5:-89574802 None:intergenic
GGATTGATCGGAACCAGAGG+AGG 0.638563 5:-89574952 None:intergenic
AAGCTCACAAGAAATTTGGA+GGG 0.638699 5:+89575879 MsG0580028957.01.T01:CDS
GGAACCAGAGGAGGAGCTGG+TGG 0.642590 5:-89574943 None:intergenic
GAAGCTTAAGAAGAAAACCA+TGG 0.645029 5:+89574680 MsG0580028957.01.T01:exon
ATCGATTCGAGGATGTGGTG+AGG 0.646651 5:-89574858 None:intergenic
GAAACTCGTCGAACTTCGAA+CGG 0.658319 5:-89574998 None:intergenic
ACTTCGAACGGAGCCACGTG+AGG 0.663920 5:-89574986 None:intergenic
GAGATTGTTGATGTGTGAGG+CGG 0.664098 5:-89574799 None:intergenic
TGTGCAGTGATTTCTTGACA+AGG 0.666059 5:+89578420 MsG0580028957.01.T01:intron
AGGTGGTGTAGTCAGCGTGA+TGG 0.671351 5:+89578454 MsG0580028957.01.T01:CDS
ACAAGGTCGTATGATGTAGG+TGG 0.691770 5:+89578437 MsG0580028957.01.T01:CDS
ATGATGGGATTAGACAGATG+AGG 0.697642 5:-89578317 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATATTTCATAATCAAT+TGG + Chr5:89576593-89576612 MsG0580028957.01.T01:intron 10.0%
!!! TACATTTATATTTTGTAATT+TGG + Chr5:89576935-89576954 MsG0580028957.01.T01:intron 10.0%
!! AATAATAACTTACAATACTA+CGG - Chr5:89575160-89575179 None:intergenic 15.0%
!!! AGAGTTTTTCATAAAATTTT+GGG - Chr5:89578526-89578545 None:intergenic 15.0%
!!! ATTTGTTAGTTTGATTTTAT+TGG + Chr5:89575251-89575270 MsG0580028957.01.T01:intron 15.0%
!!! CATTTTATGATCATTTTATA+TGG - Chr5:89576969-89576988 None:intergenic 15.0%
!!! TAGAGTTTTTCATAAAATTT+TGG - Chr5:89578527-89578546 None:intergenic 15.0%
!!! TGCATTTATATTTTGTAATT+TGG + Chr5:89576847-89576866 MsG0580028957.01.T01:intron 15.0%
!!! TTATTTTCAAAGATTTGTTT+AGG - Chr5:89577617-89577636 None:intergenic 15.0%
!! AAAGAAAATGCAAAATCAAA+AGG - Chr5:89578259-89578278 None:intergenic 20.0%
!! AAGAAAATGCAAAATCAAAA+GGG - Chr5:89578258-89578277 None:intergenic 20.0%
!! AAGAAAGAAGAAAAGATAAA+TGG - Chr5:89578083-89578102 None:intergenic 20.0%
!! AATGAGAATAACCAAATATT+TGG - Chr5:89576008-89576027 None:intergenic 20.0%
!! AGTTAAAATTTACTAAAGCT+TGG + Chr5:89577286-89577305 MsG0580028957.01.T01:CDS 20.0%
!! CACAAAACAAAACTAAAATA+GGG - Chr5:89575758-89575777 None:intergenic 20.0%
!! CATAAAATATAGAACTCAAA+AGG - Chr5:89576634-89576653 None:intergenic 20.0%
!! GAACTAAAAATACAAATTCT+TGG - Chr5:89575401-89575420 None:intergenic 20.0%
!! TAAAACAAAAAAGTCAAACT+GGG - Chr5:89577921-89577940 None:intergenic 20.0%
!! TATGCATAACAATTATGTAA+TGG - Chr5:89577229-89577248 None:intergenic 20.0%
!! TATGTTCTGTTTAATATGAA+TGG + Chr5:89578202-89578221 MsG0580028957.01.T01:intron 20.0%
!! TTAAAACAAAAAAGTCAAAC+TGG - Chr5:89577922-89577941 None:intergenic 20.0%
!! TTTAGTAATATATGACACTA+TGG + Chr5:89577452-89577471 MsG0580028957.01.T01:intron 20.0%
!!! AAAGTGAATTCTATTTGTTT+TGG + Chr5:89578150-89578169 MsG0580028957.01.T01:intron 20.0%
!!! AAGTGAATTCTATTTGTTTT+GGG + Chr5:89578151-89578170 MsG0580028957.01.T01:intron 20.0%
!!! ATTTCAAGTATATTGATGTT+CGG + Chr5:89575336-89575355 MsG0580028957.01.T01:CDS 20.0%
!!! CAGAAATTGACATTTTATTA+TGG + Chr5:89578362-89578381 MsG0580028957.01.T01:intron 20.0%
!!! TAAAAAGTGTTGTTAAATAG+AGG + Chr5:89575630-89575649 MsG0580028957.01.T01:intron 20.0%
!!! TCTTTTCAATTCCAAATATT+TGG + Chr5:89575994-89576013 MsG0580028957.01.T01:intron 20.0%
!!! TTTGCATTTTCTTTTTTACT+AGG + Chr5:89578265-89578284 MsG0580028957.01.T01:intron 20.0%
! AAAACAAAAAAGTCAAACTG+GGG - Chr5:89577920-89577939 None:intergenic 25.0%
! AGAAAATGCAAAATCAAAAG+GGG - Chr5:89578257-89578276 None:intergenic 25.0%
! ATACATCTGATTTAGAAATG+TGG + Chr5:89576517-89576536 MsG0580028957.01.T01:CDS 25.0%
! GAAAATTACAATGTAATCCA+AGG - Chr5:89577118-89577137 None:intergenic 25.0%
! GCACAAAACAAAACTAAAAT+AGG - Chr5:89575759-89575778 None:intergenic 25.0%
! GTAAATGAATTTGGATTGAT+CGG - Chr5:89574967-89574986 None:intergenic 25.0%
! TACATAATTGTTATGCATAC+AGG + Chr5:89577230-89577249 MsG0580028957.01.T01:intron 25.0%
! TACATTATTGTTACGTTTCT+TGG - Chr5:89577032-89577051 None:intergenic 25.0%
! TAGGAAATGAAATGAAAACA+AGG - Chr5:89577598-89577617 None:intergenic 25.0%
! TCATCTTCTTCTCTTTATAA+AGG - Chr5:89576156-89576175 None:intergenic 25.0%
! TGTATATCTAAGTGTTTGTT+TGG + Chr5:89577527-89577546 MsG0580028957.01.T01:intron 25.0%
! TTATAGCATATTGTATGACA+TGG - Chr5:89576243-89576262 None:intergenic 25.0%
!! AGAGTCTTGATATAACATTT+TGG + Chr5:89577769-89577788 MsG0580028957.01.T01:intron 25.0%
!! ATACATCTAGTTTCAAAACT+AGG - Chr5:89576368-89576387 None:intergenic 25.0%
!! ATGTGTTTGAAATTTCAGTA+TGG + Chr5:89577369-89577388 MsG0580028957.01.T01:intron 25.0%
!! TTGTCATTAAAAGTGTAAAC+AGG + Chr5:89577665-89577684 MsG0580028957.01.T01:intron 25.0%
!!! AGTGAATTCTATTTGTTTTG+GGG + Chr5:89578152-89578171 MsG0580028957.01.T01:intron 25.0%
!!! TTTACAGATATGCTATTTCT+AGG + Chr5:89577487-89577506 MsG0580028957.01.T01:intron 25.0%
AAAATGCAAAATCAAAAGGG+GGG - Chr5:89578255-89578274 None:intergenic 30.0%
AACCAAAAGCCTATTTAAGT+GGG - Chr5:89576884-89576903 None:intergenic 30.0%
AGAAAAGCTCACAAGAAATT+TGG + Chr5:89575875-89575894 MsG0580028957.01.T01:CDS 30.0%
AGATTAAGAAAAGCAAGCTA+AGG - Chr5:89575930-89575949 None:intergenic 30.0%
ATAAAAGGTCCTCATTAATG+GGG - Chr5:89577095-89577114 None:intergenic 30.0%
ATATGGTGTAGTACATATTG+TGG - Chr5:89576467-89576486 None:intergenic 30.0%
ATCACTGAAATCAAAACCTT+AGG + Chr5:89574759-89574778 MsG0580028957.01.T01:CDS 30.0%
ATCTAAGTGTTTGTTTGGTT+TGG + Chr5:89577532-89577551 MsG0580028957.01.T01:intron 30.0%
ATGAAAGTCAATTAAGCTCA+AGG + Chr5:89576086-89576105 MsG0580028957.01.T01:CDS 30.0%
ATTAGTTCCATCTATCTTCA+TGG + Chr5:89578553-89578572 MsG0580028957.01.T01:CDS 30.0%
CAAAAAGATATCCACATTTC+AGG - Chr5:89577802-89577821 None:intergenic 30.0%
CAATGTAATCCAAGGATAAA+AGG - Chr5:89577110-89577129 None:intergenic 30.0%
CAGTACTAAATAGCATATCA+CGG - Chr5:89575610-89575629 None:intergenic 30.0%
CATCAATCTCCAGAATATAA+TGG - Chr5:89577398-89577417 None:intergenic 30.0%
CCTTAGACAAAATACATGAT+GGG - Chr5:89578055-89578074 None:intergenic 30.0%
CTTAGACAAAATACATGATG+GGG - Chr5:89578054-89578073 None:intergenic 30.0%
GAAAATGCAAAATCAAAAGG+GGG - Chr5:89578256-89578275 None:intergenic 30.0%
GAAATCAAGTGAAATCAATG+TGG + Chr5:89577637-89577656 MsG0580028957.01.T01:intron 30.0%
GAAATGAAATGAAAACAAGG+TGG - Chr5:89577595-89577614 None:intergenic 30.0%
GATAAAAGGTCCTCATTAAT+GGG - Chr5:89577096-89577115 None:intergenic 30.0%
GCAAGAATAAAATTAACCAC+TGG - Chr5:89576127-89576146 None:intergenic 30.0%
TAAAATGCTTTCTGTAGATG+TGG + Chr5:89576982-89577001 MsG0580028957.01.T01:intron 30.0%
TAACCAAAAGCCTATTTAAG+TGG - Chr5:89576885-89576904 None:intergenic 30.0%
TCCTTAGACAAAATACATGA+TGG - Chr5:89578056-89578075 None:intergenic 30.0%
TCGTCAATGTTGTTAAATAG+CGG + Chr5:89575523-89575542 MsG0580028957.01.T01:intron 30.0%
TGAATGCGATGAAACTATTA+AGG + Chr5:89575052-89575071 MsG0580028957.01.T01:CDS 30.0%
TGTACATACGTGTATATATG+TGG + Chr5:89577735-89577754 MsG0580028957.01.T01:intron 30.0%
TTTATGTGGGATCAGTTATA+TGG - Chr5:89576783-89576802 None:intergenic 30.0%
! AAATACATGATGGGGATTTA+CGG - Chr5:89578046-89578065 None:intergenic 30.0%
! AAATCATTTAAGACCAGCTA+TGG - Chr5:89576179-89576198 None:intergenic 30.0%
! AACCAAAAGCTTGTTTATGT+GGG - Chr5:89576796-89576815 None:intergenic 30.0%
! AATTTCTTGTGAGCTTTTCT+AGG - Chr5:89575875-89575894 None:intergenic 30.0%
! ACATCTCTAATTTTTCTGCT+AGG + Chr5:89576332-89576351 MsG0580028957.01.T01:intron 30.0%
! TAACCAAAAGCTTGTTTATG+TGG - Chr5:89576797-89576816 None:intergenic 30.0%
! TCTAAGTGTTTGTTTGGTTT+GGG + Chr5:89577533-89577552 MsG0580028957.01.T01:intron 30.0%
! TGAGCTTAATTGACTTTCAT+TGG - Chr5:89576086-89576105 None:intergenic 30.0%
!! CTTGTTGTTCTAAGGAAAAA+TGG + Chr5:89576819-89576838 MsG0580028957.01.T01:intron 30.0%
!! GATCAAGTAAGAAGTATCTT+TGG + Chr5:89576722-89576741 MsG0580028957.01.T01:intron 30.0%
!! TACTTTGATGGAGTTTGTTA+AGG + Chr5:89575088-89575107 MsG0580028957.01.T01:CDS 30.0%
!! TGGTTATTCTTGTTGTTCTA+AGG + Chr5:89576811-89576830 MsG0580028957.01.T01:intron 30.0%
!! TGGTTATTCTTGTTGTTCTA+TGG + Chr5:89576811-89576830 MsG0580028957.01.T01:intron 30.0%
!!! GTGAATTCTATTTGTTTTGG+GGG + Chr5:89578153-89578172 MsG0580028957.01.T01:intron 30.0%
!!! TATCGTCTGATTTTTCAAGA+GGG - Chr5:89576500-89576519 None:intergenic 30.0%
AAAGCTCACAAGAAATTTGG+AGG + Chr5:89575878-89575897 MsG0580028957.01.T01:CDS 35.0%
AAATAGCATATCACGGATAG+CGG - Chr5:89575603-89575622 None:intergenic 35.0%
AAATGCAAAATCAAAAGGGG+GGG - Chr5:89578254-89578273 None:intergenic 35.0%
AAGCTCACAAGAAATTTGGA+GGG + Chr5:89575879-89575898 MsG0580028957.01.T01:CDS 35.0%
AATGTCAATTTCTGTAGCAC+AGG - Chr5:89578356-89578375 None:intergenic 35.0%
ACGTATGTACAGTTGTATTC+AGG - Chr5:89577726-89577745 None:intergenic 35.0%
AGGTGTTTACCATAACATGA+TGG - Chr5:89578336-89578355 None:intergenic 35.0%
AGTTGTGCTTGATACTTTGA+TGG + Chr5:89575076-89575095 MsG0580028957.01.T01:CDS 35.0%
ATAGCTGATCCCACTTAAAT+AGG + Chr5:89576872-89576891 MsG0580028957.01.T01:intron 35.0%
CCTCATTAATGGGGAAAAAA+GGG - Chr5:89577086-89577105 None:intergenic 35.0%
CTCATTAATGGGGAAAAAAG+GGG - Chr5:89577085-89577104 None:intergenic 35.0%
GAAGCTTAAGAAGAAAACCA+TGG + Chr5:89574680-89574699 MsG0580028957.01.T01:exon 35.0%
GGATAAAAGGTCCTCATTAA+TGG - Chr5:89577097-89577116 None:intergenic 35.0%
GGTGTTTACCATAACATGAT+GGG - Chr5:89578335-89578354 None:intergenic 35.0%
GTATGGTTGCCATTATATTC+TGG + Chr5:89577386-89577405 MsG0580028957.01.T01:intron 35.0%
GTCAAAGTCAAATCCATTGT+TGG + Chr5:89577855-89577874 MsG0580028957.01.T01:intron 35.0%
GTGTAAACAGGAATGAATGT+AGG + Chr5:89577677-89577696 MsG0580028957.01.T01:intron 35.0%
TAACCAAACATTTCTCCACT+TGG + Chr5:89577992-89578011 MsG0580028957.01.T01:intron 35.0%
TATGTACAGTTGTATTCAGG+TGG - Chr5:89577723-89577742 None:intergenic 35.0%
TCATTAATGGGGAAAAAAGG+GGG - Chr5:89577084-89577103 None:intergenic 35.0%
TCCTCATTAATGGGGAAAAA+AGG - Chr5:89577087-89577106 None:intergenic 35.0%
TGTCTAATCCCATCATGTTA+TGG + Chr5:89578324-89578343 MsG0580028957.01.T01:CDS 35.0%
TTAGAAATGTGGAGTGTATC+AGG + Chr5:89576528-89576547 MsG0580028957.01.T01:CDS 35.0%
TTAGTAATGCTATAGCGTAG+CGG + Chr5:89575553-89575572 MsG0580028957.01.T01:intron 35.0%
TTCCTACTATAGACCAACAA+TGG - Chr5:89577871-89577890 None:intergenic 35.0%
TTTCTTCATTGCAGTTTCCA+TGG - Chr5:89574700-89574719 None:intergenic 35.0%
TTTGTTAAGGTTGCTAATGG+TGG + Chr5:89575101-89575120 MsG0580028957.01.T01:CDS 35.0%
! AATGGATTTGACTTTGACCT+TGG - Chr5:89577853-89577872 None:intergenic 35.0%
! ATCACTTATCGATTGTCTTG+TGG + Chr5:89575025-89575044 MsG0580028957.01.T01:CDS 35.0%
! ATCCATTGTTGGTCTATAGT+AGG + Chr5:89577866-89577885 MsG0580028957.01.T01:intron 35.0%
! ATCCCACATAAACAAGCTTT+TGG + Chr5:89576791-89576810 MsG0580028957.01.T01:intron 35.0%
! ATCCCACTTAAATAGGCTTT+TGG + Chr5:89576879-89576898 MsG0580028957.01.T01:intron 35.0%
! CCCATCATGTATTTTGTCTA+AGG + Chr5:89578052-89578071 MsG0580028957.01.T01:intron 35.0%
! GCTATAAAGCTGTCACTTAT+TGG - Chr5:89576750-89576769 None:intergenic 35.0%
! TAATGAGGACCTTTTATCCT+TGG + Chr5:89577098-89577117 MsG0580028957.01.T01:intron 35.0%
! TGTTTTGCCATGAAGATAGA+TGG - Chr5:89578563-89578582 None:intergenic 35.0%
!! GAGTTTGTTAAGGTTGCTAA+TGG + Chr5:89575098-89575117 MsG0580028957.01.T01:CDS 35.0%
!!! ATCGTCTGATTTTTCAAGAG+GGG - Chr5:89576499-89576518 None:intergenic 35.0%
!!! GTATCGTCTGATTTTTCAAG+AGG - Chr5:89576501-89576520 None:intergenic 35.0%
!!! TTTTTTGCTTAGCGCCTTTA+TGG + Chr5:89575472-89575491 MsG0580028957.01.T01:intron 35.0%
AAGAGGGGGAATATGTGATA+TGG - Chr5:89576484-89576503 None:intergenic 40.0%
AAGCTTGGATTGCGTATCTT+AGG + Chr5:89577301-89577320 MsG0580028957.01.T01:CDS 40.0%
ACCTCTTTGAACACATCCAA+TGG - Chr5:89577336-89577355 None:intergenic 40.0%
ACTATAGCACTGTTGCATAG+GGG + Chr5:89575654-89575673 MsG0580028957.01.T01:intron 40.0%
CACTATAGCACTGTTGCATA+GGG + Chr5:89575653-89575672 MsG0580028957.01.T01:intron 40.0%
CATATTGTGGATGATGCACT+CGG - Chr5:89576454-89576473 None:intergenic 40.0%
CATGAATCGTGTCATATGCA+GGG + Chr5:89575795-89575814 MsG0580028957.01.T01:intron 40.0%
CCAAATTCATTTACCTCACG+TGG + Chr5:89574973-89574992 MsG0580028957.01.T01:CDS 40.0%
CCACGTGAGGTAAATGAATT+TGG - Chr5:89574976-89574995 None:intergenic 40.0%
CTAGTTTCAAAACTAGGACC+AGG - Chr5:89576362-89576381 None:intergenic 40.0%
CTTCATCTTCTTCGCAATTC+TGG - Chr5:89577268-89577287 None:intergenic 40.0%
GCTGGGTCATTAGGATATAA+AGG - Chr5:89578490-89578509 None:intergenic 40.0%
TATATCCTAATGACCCAGCA+TGG + Chr5:89578491-89578510 MsG0580028957.01.T01:CDS 40.0%
TCATGAATCGTGTCATATGC+AGG + Chr5:89575794-89575813 MsG0580028957.01.T01:intron 40.0%
TCCATTGGATGTGTTCAAAG+AGG + Chr5:89577332-89577351 MsG0580028957.01.T01:CDS 40.0%
TGAGAACTTCCCTTCCATAA+AGG - Chr5:89575489-89575508 None:intergenic 40.0%
TGTGCAGTGATTTCTTGACA+AGG + Chr5:89578420-89578439 MsG0580028957.01.T01:intron 40.0%
TTGAACACATCCAATGGAAG+TGG - Chr5:89577330-89577349 None:intergenic 40.0%
TTGACAAGGTCGTATGATGT+AGG + Chr5:89578434-89578453 MsG0580028957.01.T01:CDS 40.0%
TTGGAGGGAAAAGATGTTTC+AGG + Chr5:89575894-89575913 MsG0580028957.01.T01:CDS 40.0%
TTTATGGAAGGGAAGTTCTC+AGG + Chr5:89575488-89575507 MsG0580028957.01.T01:intron 40.0%
! ACTTATCGATTGTCTTGTGG+CGG + Chr5:89575028-89575047 MsG0580028957.01.T01:CDS 40.0%
! ATGATGGGATTAGACAGATG+AGG - Chr5:89578320-89578339 None:intergenic 40.0%
! TTGGTAGTAGTCCTGAAATG+TGG + Chr5:89577788-89577807 MsG0580028957.01.T01:intron 40.0%
!! ACATCACCTACTTTTCTGCT+AGG + Chr5:89576305-89576324 MsG0580028957.01.T01:intron 40.0%
!!! CCCTTTTTTCCCCATTAATG+AGG + Chr5:89577083-89577102 MsG0580028957.01.T01:intron 40.0%
!!! TCAGTGATGCTGTGTTGTTT+TGG - Chr5:89574747-89574766 None:intergenic 40.0%
!!! TCGTCTGATTTTTCAAGAGG+GGG - Chr5:89576498-89576517 None:intergenic 40.0%
AAACATTTCTCCACTTGGCG+GGG + Chr5:89577997-89578016 MsG0580028957.01.T01:intron 45.0%
AAGAAGGGGAGATGAATGTG+AGG - Chr5:89574833-89574852 None:intergenic 45.0%
ACAAGGTCGTATGATGTAGG+TGG + Chr5:89578437-89578456 MsG0580028957.01.T01:CDS 45.0%
ACAGATGAGGAATCACTGCT+TGG - Chr5:89578307-89578326 None:intergenic 45.0%
AGATGAATGTGAGGAGGAGA+GGG - Chr5:89574824-89574843 None:intergenic 45.0%
AGGGAGATTGTTGATGTGTG+AGG - Chr5:89574805-89574824 None:intergenic 45.0%
CAAACATTTCTCCACTTGGC+GGG + Chr5:89577996-89578015 MsG0580028957.01.T01:intron 45.0%
CAATCTGCGTTTGACAACAG+TGG + Chr5:89575702-89575721 MsG0580028957.01.T01:intron 45.0%
CCAAACATTTCTCCACTTGG+CGG + Chr5:89577995-89578014 MsG0580028957.01.T01:intron 45.0%
GAAACTCGTCGAACTTCGAA+CGG - Chr5:89575001-89575020 None:intergenic 45.0%
GAGAAGAAGATGACCATAGC+TGG + Chr5:89576163-89576182 MsG0580028957.01.T01:intron 45.0%
GAGATTGTTGATGTGTGAGG+CGG - Chr5:89574802-89574821 None:intergenic 45.0%
GATAAAACTGCAAGCGCAGA+TGG + Chr5:89576058-89576077 MsG0580028957.01.T01:CDS 45.0%
GCACTATAGCACTGTTGCAT+AGG + Chr5:89575652-89575671 MsG0580028957.01.T01:intron 45.0%
GGAGAGAAGTTCTTGACCTA+AGG - Chr5:89574778-89574797 None:intergenic 45.0%
GGGAGCAACATTAACGAGTA+CGG - Chr5:89577064-89577083 None:intergenic 45.0%
GTTAGTAAGACTCTAGCCAG+TGG + Chr5:89576108-89576127 MsG0580028957.01.T01:intron 45.0%
GTTCTGCCTAGCAGAAAAGT+AGG - Chr5:89576314-89576333 None:intergenic 45.0%
TCATGGCAAAACATCGTGCA+AGG + Chr5:89578570-89578589 MsG0580028957.01.T01:CDS 45.0%
TGCTATTAAAGCCCCTGTCA+TGG + Chr5:89577820-89577839 MsG0580028957.01.T01:intron 45.0%
TGCTTAGCGCCTTTATGGAA+GGG + Chr5:89575477-89575496 MsG0580028957.01.T01:intron 45.0%
TGGGTATTCCAATCCATGCT+GGG - Chr5:89578507-89578526 None:intergenic 45.0%
TTGCTTAGCGCCTTTATGGA+AGG + Chr5:89575476-89575495 MsG0580028957.01.T01:intron 45.0%
TTGGGTATTCCAATCCATGC+TGG - Chr5:89578508-89578527 None:intergenic 45.0%
TTTGGATTGATCGGAACCAG+AGG - Chr5:89574958-89574977 None:intergenic 45.0%
! ACAGTGGTCAGCGTGTTATT+TGG + Chr5:89575718-89575737 MsG0580028957.01.T01:intron 45.0%
! ACATCGTGCAAGGTTTTTCC+AGG + Chr5:89578580-89578599 MsG0580028957.01.T01:CDS 45.0%
! ATTTTTCTGCTAGGCAGACC+TGG + Chr5:89576341-89576360 MsG0580028957.01.T01:intron 45.0%
! TCTTAGGTTGCCACTTCCAT+TGG + Chr5:89577317-89577336 MsG0580028957.01.T01:CDS 45.0%
!! AGAGAATCGATTCGAGGATG+TGG - Chr5:89574866-89574885 None:intergenic 45.0%
!! GAAGAGAGAGAATCGATTCG+AGG - Chr5:89574872-89574891 None:intergenic 45.0%
!! TTAAAAAAAATCATATAAAA+AGG - Chr5:89575378-89575397 None:intergenic 5.0%
AAGGGGAGATGAATGTGAGG+AGG - Chr5:89574830-89574849 None:intergenic 50.0%
AGTAGGAACCTGTGACCTTC+TGG + Chr5:89577883-89577902 MsG0580028957.01.T01:intron 50.0%
ATGTGGTGAGGTGGAGAAGA+AGG - Chr5:89574849-89574868 None:intergenic 50.0%
ATTAAAGCCCCTGTCATGGC+AGG + Chr5:89577824-89577843 MsG0580028957.01.T01:intron 50.0%
ATTGTTGATGTGTGAGGCGG+AGG - Chr5:89574799-89574818 None:intergenic 50.0%
CCAATCCATGCTGGGTCATT+AGG - Chr5:89578499-89578518 None:intergenic 50.0%
CCGCCAAGTGGAGAAATGTT+TGG - Chr5:89577998-89578017 None:intergenic 50.0%
CCTAATGACCCAGCATGGAT+TGG + Chr5:89578496-89578515 MsG0580028957.01.T01:CDS 50.0%
GAGATGAATGTGAGGAGGAG+AGG - Chr5:89574825-89574844 None:intergenic 50.0%
TGTGGTGAGGTGGAGAAGAA+GGG - Chr5:89574848-89574867 None:intergenic 50.0%
TTAAAGCCCCTGTCATGGCA+GGG + Chr5:89577825-89577844 MsG0580028957.01.T01:intron 50.0%
TTGTGTGACCAGAAGGTCAC+AGG - Chr5:89577894-89577913 None:intergenic 50.0%
! GAGGAATCACTGCTTGGTGA+AGG - Chr5:89578301-89578320 None:intergenic 50.0%
!! ATCGATTCGAGGATGTGGTG+AGG - Chr5:89574861-89574880 None:intergenic 50.0%
!! GTGGTCAGCGTGTTATTTGG+TGG + Chr5:89575721-89575740 MsG0580028957.01.T01:intron 50.0%
AAGGGAGTTGAGGGAGGAGA+GGG - Chr5:89574908-89574927 None:intergenic 55.0%
AGGTGGTGTAGTCAGCGTGA+TGG + Chr5:89578454-89578473 MsG0580028957.01.T01:CDS 55.0%
ATTTCTCCACTTGGCGGGGT+TGG + Chr5:89578001-89578020 MsG0580028957.01.T01:intron 55.0%
CTTGGAACCCTGCCATGACA+GGG - Chr5:89577835-89577854 None:intergenic 55.0%
GATTCGAGGATGTGGTGAGG+TGG - Chr5:89574858-89574877 None:intergenic 55.0%
GGATTGATCGGAACCAGAGG+AGG - Chr5:89574955-89574974 None:intergenic 55.0%
GGGGCTATTGTGTGACCAGA+AGG - Chr5:89577901-89577920 None:intergenic 55.0%
GTGGTGAGGTGGAGAAGAAG+GGG - Chr5:89574847-89574866 None:intergenic 55.0%
TTGGAACCCTGCCATGACAG+GGG - Chr5:89577834-89577853 None:intergenic 55.0%
ACTTCGAACGGAGCCACGTG+AGG - Chr5:89574989-89575008 None:intergenic 60.0%
AGGGAGGAGAGGGAGGAAGA+AGG - Chr5:89574898-89574917 None:intergenic 60.0%
ATCGGAACCAGAGGAGGAGC+TGG - Chr5:89574949-89574968 None:intergenic 60.0%
ATGTAGCCAACCCCGCCAAG+TGG - Chr5:89578010-89578029 None:intergenic 60.0%
CCTGTCATGGCAGGGTTCCA+AGG + Chr5:89577833-89577852 MsG0580028957.01.T01:intron 60.0%
CCTTGGAACCCTGCCATGAC+AGG - Chr5:89577836-89577855 None:intergenic 60.0%
GAAGGGAGTTGAGGGAGGAG+AGG - Chr5:89574909-89574928 None:intergenic 60.0%
!! ACTTGGCGGGGTTGGCTACA+TGG + Chr5:89578009-89578028 MsG0580028957.01.T01:intron 60.0%
ACCACCACCAGCTCCTCCTC+TGG + Chr5:89574939-89574958 MsG0580028957.01.T01:CDS 65.0%
ACCAGAGGAGGAGCTGGTGG+TGG - Chr5:89574943-89574962 None:intergenic 65.0%
GGAACCAGAGGAGGAGCTGG+TGG - Chr5:89574946-89574965 None:intergenic 65.0%
GGAGTTGAGGGAGGAGAGGG+AGG - Chr5:89574905-89574924 None:intergenic 65.0%
TGGCGGAAGGGAGTTGAGGG+AGG - Chr5:89574914-89574933 None:intergenic 65.0%
!! CGGTGGCGGAAGGGAGTTGA+GGG - Chr5:89574917-89574936 None:intergenic 65.0%
!! GGAGGAGCTGGTGGTGGTTG+CGG - Chr5:89574937-89574956 None:intergenic 65.0%
!! TGGTGGTTGCGGTGGCGGAA+GGG - Chr5:89574926-89574945 None:intergenic 65.0%
!! GCGGTGGCGGAAGGGAGTTG+AGG - Chr5:89574918-89574937 None:intergenic 70.0%
!! GCTGGTGGTGGTTGCGGTGG+CGG - Chr5:89574931-89574950 None:intergenic 70.0%
!! GGAGCTGGTGGTGGTTGCGG+TGG - Chr5:89574934-89574953 None:intergenic 70.0%
!! GTGGTGGTTGCGGTGGCGGA+AGG - Chr5:89574927-89574946 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 89574679 89578619 89574679 ID=MsG0580028957.01;Name=MsG0580028957.01
Chr5 mRNA 89574679 89578619 89574679 ID=MsG0580028957.01.T01;Parent=MsG0580028957.01;Name=MsG0580028957.01.T01;_AED=0.43;_eAED=0.48;_QI=20|0.77|0.6|1|0.55|0.6|10|0|420
Chr5 exon 89574679 89575166 89574679 ID=MsG0580028957.01.T01:exon:25965;Parent=MsG0580028957.01.T01
Chr5 exon 89575284 89575357 89575284 ID=MsG0580028957.01.T01:exon:25966;Parent=MsG0580028957.01.T01
Chr5 exon 89575854 89575915 89575854 ID=MsG0580028957.01.T01:exon:25967;Parent=MsG0580028957.01.T01
Chr5 exon 89576056 89576111 89576056 ID=MsG0580028957.01.T01:exon:25968;Parent=MsG0580028957.01.T01
Chr5 exon 89576226 89576289 89576226 ID=MsG0580028957.01.T01:exon:25969;Parent=MsG0580028957.01.T01
Chr5 exon 89576415 89576549 89576415 ID=MsG0580028957.01.T01:exon:25970;Parent=MsG0580028957.01.T01
Chr5 exon 89576999 89577048 89576999 ID=MsG0580028957.01.T01:exon:25971;Parent=MsG0580028957.01.T01
Chr5 exon 89577252 89577353 89577252 ID=MsG0580028957.01.T01:exon:25972;Parent=MsG0580028957.01.T01
Chr5 exon 89578287 89578345 89578287 ID=MsG0580028957.01.T01:exon:25973;Parent=MsG0580028957.01.T01
Chr5 exon 89578427 89578619 89578427 ID=MsG0580028957.01.T01:exon:25974;Parent=MsG0580028957.01.T01
Chr5 five_prime_UTR 89574679 89574698 89574679 ID=MsG0580028957.01.T01:five_prime_utr;Parent=MsG0580028957.01.T01
Chr5 CDS 89574699 89575166 89574699 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89575284 89575357 89575284 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89575854 89575915 89575854 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89576056 89576111 89576056 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89576226 89576289 89576226 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89576415 89576549 89576415 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89576999 89577048 89576999 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89577252 89577353 89577252 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89578287 89578345 89578287 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Chr5 CDS 89578427 89578619 89578427 ID=MsG0580028957.01.T01:cds;Parent=MsG0580028957.01.T01
Gene Sequence

>MsG0580028957.01.T01

ATGGAAACTGCAATGAAGAAAACGAAGAAGATGAAGAAGAAAACACCAAAACAACACAGCATCACTGAAATCAAAACCTTAGGTCAAGAACTTCTCTCCTCCGCCTCACACATCAACAATCTCCCTCTCCTCCTCACATTCATCTCCCCTTCTTCTCCACCTCACCACATCCTCGAATCGATTCTCTCTCTTCACTCCTTCTTCCTCCCTCTCCTCCCTCAACTCCCTTCCGCCACCGCAACCACCACCAGCTCCTCCTCTGGTTCCGATCAATCCAAATTCATTTACCTCACGTGGCTCCGTTCGAAGTTCGACGAGTTTCTCAAATCACTTATCGATTGTCTTGTGGCGGATGAATGCGATGAAACTATTAAGGAAGTTGTGCTTGATACTTTGATGGAGTTTGTTAAGGTTGCTAATGGTGGAGCATTTCATTCTTCATTATATAACAGAATTCTCCGTAGTATTATTCACTCTACTAGTTCTGCTGAATTTTTGATTGATTTACTAATATCGAAGTATTTCAAGTATATTGATGTTCGTTATTTTACATTCATAAGCCTAGAAAAGCTCACAAGAAATTTGGAGGGAAAAGATGTTTCAGATGATAAAACTGCAAGCGCAGATGGCACCAATGAAAGTCAATTAAGCTCAAGGTTAACAAAAATTGCGTGCCATGTCATACAATATGCTATAAGCAATATGTTTAGCTTTGCAACTCAATTTCTATTGATAACTTGTAAATGTATCTTTTGCAGTACCGAGTGCATCATCCACAATATGTACTACACCATATCACATATTCCCCCTCTTGAAAAATCAGACGATACATCTGATTTAGAAATGTGGAGTGTATCAGATGTGGATGCAGATGATAAGCAGCTTAAGTCCAAGAAACGTAACAATAATGTATTGTCTGCAGCCAGAATTGCGAAGAAGATGAAGTTAAAATTTACTAAAGCTTGGATTGCGTATCTTAGGTTGCCACTTCCATTGGATGTGTTCAAAGAGGTTCTTGTAAACCTTCACCAAGCAGTGATTCCTCATCTGTCTAATCCCATCATGTTATGTGATTTCTTGACAAGGTCGTATGATGTAGGTGGTGTAGTCAGCGTGATGGCTCTTAATAGCCTTTATATCCTAATGACCCAGCATGGATTGGAATACCCAAAATTTTATGAAAAACTCTATGCACTATTAGTTCCATCTATCTTCATGGCAAAACATCGTGCAAGGTTTTTCCAGGTAAAGCTTATAAAATGA
Protein sequence

>MsG0580028957.01.T01

METAMKKTKKMKKKTPKQHSITEIKTLGQELLSSASHINNLPLLLTFISPSSPPHHILESILSLHSFFLPLLPQLPSATATTTSSSSGSDQSKFIYLTWLRSKFDEFLKSLIDCLVADECDETIKEVVLDTLMEFVKVANGGAFHSSLYNRILRSIIHSTSSAEFLIDLLISKYFKYIDVRYFTFISLEKLTRNLEGKDVSDDKTASADGTNESQLSSRLTKIACHVIQYAISNMFSFATQFLLITCKCIFCSTECIIHNMYYTISHIPPLEKSDDTSDLEMWSVSDVDADDKQLKSKKRNNNVLSAARIAKKMKLKFTKAWIAYLRLPLPLDVFKEVLVNLHQAVIPHLSNPIMLCDFLTRSYDVGGVVSVMALNSLYILMTQHGLEYPKFYEKLYALLVPSIFMAKHRARFFQVKLIK*