Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000400.01 | MsG0380016812.01 | 0.810497 | 1.119290e-50 | 6.757406e-48 |
| MsG0180000854.01 | MsG0380016812.01 | -0.839116 | 1.920957e-57 | 2.626536e-54 |
| MsG0180004399.01 | MsG0380016812.01 | -0.851299 | 9.713905e-61 | 1.957522e-57 |
| MsG0380015804.01 | MsG0380016812.01 | -0.811398 | 7.143850e-51 | 4.417443e-48 |
| MsG0380016810.01 | MsG0380016812.01 | 0.822855 | 1.894777e-53 | 1.604876e-50 |
| MsG0380016812.01 | MsG0480021141.01 | -0.818516 | 1.881914e-52 | 1.411743e-49 |
| MsG0380016812.01 | MsG0580026786.01 | 0.818477 | 1.920659e-52 | 1.439286e-49 |
| MsG0380016812.01 | MsG0680031130.01 | 0.811556 | 6.598698e-51 | 4.097426e-48 |
| MsG0380016812.01 | MsG0680032814.01 | -0.805496 | 1.298163e-49 | 6.878025e-47 |
| MsG0280007445.01 | MsG0380016812.01 | 0.806026 | 1.004515e-49 | 5.395318e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTGGTCCCCTATGGTTT+TGG | 0.176501 | 3:-88164676 | None:intergenic |
| TCTCTTTCCATCTGGGCTTC+TGG | 0.187458 | 3:+88164749 | MsG0380016812.01.T01:CDS |
| TCGAGCTCGGTAGCGTGGGC+TGG | 0.310680 | 3:+88164964 | MsG0380016812.01.T01:CDS |
| ACCAAGCCTCTCTTTCCATC+TGG | 0.320650 | 3:+88164741 | MsG0380016812.01.T01:CDS |
| ACTGGGGAGCGAATGCGAAC+TGG | 0.377768 | 3:+88164920 | MsG0380016812.01.T01:CDS |
| CGGGCCAAAGGTGAAATTCT+TGG | 0.382916 | 3:-88164692 | None:intergenic |
| ATTTCACTGGACTCCTGCTC+CGG | 0.404898 | 3:+88164835 | MsG0380016812.01.T01:CDS |
| TTTCACCTTTGGCCCGAGTC+AGG | 0.412665 | 3:+88164699 | MsG0380016812.01.T01:CDS |
| CGAGCTCGGTAGCGTGGGCT+GGG | 0.420545 | 3:+88164965 | MsG0380016812.01.T01:CDS |
| TTCGCATTCGCTCCCCAGTT+CGG | 0.421685 | 3:-88164916 | None:intergenic |
| GCAGGAGTCCAGTGAAATCT+AGG | 0.423588 | 3:-88164830 | None:intergenic |
| GTGAAATTCTTGGTCCCCTA+TGG | 0.428064 | 3:-88164682 | None:intergenic |
| GGAGAGGGGCGAGATCGATC+GGG | 0.428321 | 3:+88164884 | MsG0380016812.01.T01:CDS |
| AGGAGAGGGGCGAGATCGAT+CGG | 0.435576 | 3:+88164883 | MsG0380016812.01.T01:CDS |
| CCAAGCCTCTCTTTCCATCT+GGG | 0.443449 | 3:+88164742 | MsG0380016812.01.T01:CDS |
| CTGGGGAGCGAATGCGAACT+GGG | 0.445099 | 3:+88164921 | MsG0380016812.01.T01:CDS |
| GGGACCAAGAATTTCACCTT+TGG | 0.450099 | 3:+88164688 | MsG0380016812.01.T01:CDS |
| CGGCGATGGTTCTCCTGACT+CGG | 0.465118 | 3:-88164712 | None:intergenic |
| CGCCCCTCTCCTGTGTAGAA+AGG | 0.466075 | 3:-88164872 | None:intergenic |
| ATCTTCGAGCTCGGTAGCGT+GGG | 0.489627 | 3:+88164960 | MsG0380016812.01.T01:CDS |
| ATGGTGATCGGCTTCTCCTT+CGG | 0.490001 | 3:+88164996 | MsG0380016812.01.T01:CDS |
| ACAGAAGACCTAGATTTCAC+TGG | 0.493926 | 3:+88164822 | MsG0380016812.01.T01:CDS |
| AAAGGCATTCTTCGTACTCC+CGG | 0.505081 | 3:-88164854 | None:intergenic |
| ATTCTTCGTACTCCCGGAGC+AGG | 0.508408 | 3:-88164848 | None:intergenic |
| AGCGAATGCGAACTGGGCTG+CGG | 0.509557 | 3:+88164927 | MsG0380016812.01.T01:CDS |
| CAACAGTACCCAAAACCATA+GGG | 0.531341 | 3:+88164667 | MsG0380016812.01.T01:CDS |
| GAGAGGGGCGAGATCGATCG+GGG | 0.536427 | 3:+88164885 | MsG0380016812.01.T01:CDS |
| ATGCCTTTCTACACAGGAGA+GGG | 0.541516 | 3:+88164869 | MsG0380016812.01.T01:CDS |
| GTTCTCCTGACTCGGGCCAA+AGG | 0.543507 | 3:-88164704 | None:intergenic |
| CGTGGGCTGGGCGAAACAGA+TGG | 0.544805 | 3:+88164977 | MsG0380016812.01.T01:CDS |
| TTTCACTGGACTCCTGCTCC+GGG | 0.544930 | 3:+88164836 | MsG0380016812.01.T01:CDS |
| AAGAGAGGCTTGGTCTCATA+CGG | 0.546509 | 3:-88164732 | None:intergenic |
| CACGAAAAGCAAGTAGAGAC+AGG | 0.548672 | 3:-88164785 | None:intergenic |
| AGAAGCCCAGATGGAAAGAG+AGG | 0.548943 | 3:-88164747 | None:intergenic |
| TCATCATCCAGAAGCCCAGA+TGG | 0.554328 | 3:-88164756 | None:intergenic |
| ACAACAGTACCCAAAACCAT+AGG | 0.556486 | 3:+88164666 | MsG0380016812.01.T01:CDS |
| TATCTTCGAGCTCGGTAGCG+TGG | 0.562882 | 3:+88164959 | MsG0380016812.01.T01:CDS |
| CCCAGATGGAAAGAGAGGCT+TGG | 0.563233 | 3:-88164742 | None:intergenic |
| TGGGCGAAACAGATGGTGAT+CGG | 0.566190 | 3:+88164984 | MsG0380016812.01.T01:CDS |
| CGAAGAATGCCTTTCTACAC+AGG | 0.578528 | 3:+88164863 | MsG0380016812.01.T01:CDS |
| TTCTGCTATATCTTCGAGCT+CGG | 0.582459 | 3:+88164951 | MsG0380016812.01.T01:CDS |
| GGCTTGGTCTCATACGGCGA+TGG | 0.588268 | 3:-88164726 | None:intergenic |
| GGAATATACTTAGGTACCGA+AGG | 0.599526 | 3:-88165012 | None:intergenic |
| GGCGATGGTTCTCCTGACTC+GGG | 0.602378 | 3:-88164711 | None:intergenic |
| AGAGGGGCGAGATCGATCGG+GGG | 0.622482 | 3:+88164886 | MsG0380016812.01.T01:CDS |
| TGCCTTTCTACACAGGAGAG+GGG | 0.673060 | 3:+88164870 | MsG0380016812.01.T01:CDS |
| AATGCCTTTCTACACAGGAG+AGG | 0.680912 | 3:+88164868 | MsG0380016812.01.T01:CDS |
| AACAGTACCCAAAACCATAG+GGG | 0.683927 | 3:+88164668 | MsG0380016812.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAGTACCCAAAACCATAG+GGG | + | Chr3:88164668-88164687 | MsG0380016812.01.T01:CDS | 40.0% | |
| ACAACAGTACCCAAAACCAT+AGG | + | Chr3:88164666-88164685 | MsG0380016812.01.T01:CDS | 40.0% | |
| ACAGAAGACCTAGATTTCAC+TGG | + | Chr3:88164822-88164841 | MsG0380016812.01.T01:CDS | 40.0% | |
| CAACAGTACCCAAAACCATA+GGG | + | Chr3:88164667-88164686 | MsG0380016812.01.T01:CDS | 40.0% | |
| TTCTGCTATATCTTCGAGCT+CGG | + | Chr3:88164951-88164970 | MsG0380016812.01.T01:CDS | 40.0% | |
| AATGCCTTTCTACACAGGAG+AGG | + | Chr3:88164868-88164887 | MsG0380016812.01.T01:CDS | 45.0% | |
| ATGCCTTTCTACACAGGAGA+GGG | + | Chr3:88164869-88164888 | MsG0380016812.01.T01:CDS | 45.0% | |
| CACGAAAAGCAAGTAGAGAC+AGG | - | Chr3:88164788-88164807 | None:intergenic | 45.0% | |
| CGAAGAATGCCTTTCTACAC+AGG | + | Chr3:88164863-88164882 | MsG0380016812.01.T01:CDS | 45.0% | |
| GGGACCAAGAATTTCACCTT+TGG | + | Chr3:88164688-88164707 | MsG0380016812.01.T01:CDS | 45.0% | |
| GTGAAATTCTTGGTCCCCTA+TGG | - | Chr3:88164685-88164704 | None:intergenic | 45.0% | |
| ! | AAGAGAGGCTTGGTCTCATA+CGG | - | Chr3:88164735-88164754 | None:intergenic | 45.0% |
| ! | TCTTGGTCCCCTATGGTTTT+GGG | - | Chr3:88164678-88164697 | None:intergenic | 45.0% |
| ! | TTCTTGGTCCCCTATGGTTT+TGG | - | Chr3:88164679-88164698 | None:intergenic | 45.0% |
| !! | AAAGGCATTCTTCGTACTCC+CGG | - | Chr3:88164857-88164876 | None:intergenic | 45.0% |
| !! | CTACTTGCTTTTCGTGGAGA+TGG | + | Chr3:88164792-88164811 | MsG0380016812.01.T01:CDS | 45.0% |
| !! | CTGTCTCTACTTGCTTTTCG+TGG | + | Chr3:88164786-88164805 | MsG0380016812.01.T01:CDS | 45.0% |
| ACCAAGCCTCTCTTTCCATC+TGG | + | Chr3:88164741-88164760 | MsG0380016812.01.T01:CDS | 50.0% | |
| AGAAGCCCAGATGGAAAGAG+AGG | - | Chr3:88164750-88164769 | None:intergenic | 50.0% | |
| ATTTCACTGGACTCCTGCTC+CGG | + | Chr3:88164835-88164854 | MsG0380016812.01.T01:CDS | 50.0% | |
| CCAAGCCTCTCTTTCCATCT+GGG | + | Chr3:88164742-88164761 | MsG0380016812.01.T01:CDS | 50.0% | |
| CGGGCCAAAGGTGAAATTCT+TGG | - | Chr3:88164695-88164714 | None:intergenic | 50.0% | |
| GCAGGAGTCCAGTGAAATCT+AGG | - | Chr3:88164833-88164852 | None:intergenic | 50.0% | |
| TCATCATCCAGAAGCCCAGA+TGG | - | Chr3:88164759-88164778 | None:intergenic | 50.0% | |
| TCTCTTTCCATCTGGGCTTC+TGG | + | Chr3:88164749-88164768 | MsG0380016812.01.T01:CDS | 50.0% | |
| TGCCTTTCTACACAGGAGAG+GGG | + | Chr3:88164870-88164889 | MsG0380016812.01.T01:CDS | 50.0% | |
| TGGGCGAAACAGATGGTGAT+CGG | + | Chr3:88164984-88165003 | MsG0380016812.01.T01:CDS | 50.0% | |
| ! | ATGGTGATCGGCTTCTCCTT+CGG | + | Chr3:88164996-88165015 | MsG0380016812.01.T01:CDS | 50.0% |
| ATCTTCGAGCTCGGTAGCGT+GGG | + | Chr3:88164960-88164979 | MsG0380016812.01.T01:CDS | 55.0% | |
| ATTCTTCGTACTCCCGGAGC+AGG | - | Chr3:88164851-88164870 | None:intergenic | 55.0% | |
| CCCAGATGGAAAGAGAGGCT+TGG | - | Chr3:88164745-88164764 | None:intergenic | 55.0% | |
| TATCTTCGAGCTCGGTAGCG+TGG | + | Chr3:88164959-88164978 | MsG0380016812.01.T01:CDS | 55.0% | |
| TTCGCATTCGCTCCCCAGTT+CGG | - | Chr3:88164919-88164938 | None:intergenic | 55.0% | |
| TTTCACCTTTGGCCCGAGTC+AGG | + | Chr3:88164699-88164718 | MsG0380016812.01.T01:CDS | 55.0% | |
| TTTCACTGGACTCCTGCTCC+GGG | + | Chr3:88164836-88164855 | MsG0380016812.01.T01:CDS | 55.0% | |
| ACTGGGGAGCGAATGCGAAC+TGG | + | Chr3:88164920-88164939 | MsG0380016812.01.T01:CDS | 60.0% | |
| AGCGAATGCGAACTGGGCTG+CGG | + | Chr3:88164927-88164946 | MsG0380016812.01.T01:CDS | 60.0% | |
| AGGAGAGGGGCGAGATCGAT+CGG | + | Chr3:88164883-88164902 | MsG0380016812.01.T01:CDS | 60.0% | |
| CGCCCCTCTCCTGTGTAGAA+AGG | - | Chr3:88164875-88164894 | None:intergenic | 60.0% | |
| CGGCGATGGTTCTCCTGACT+CGG | - | Chr3:88164715-88164734 | None:intergenic | 60.0% | |
| CTGGGGAGCGAATGCGAACT+GGG | + | Chr3:88164921-88164940 | MsG0380016812.01.T01:CDS | 60.0% | |
| GGCGATGGTTCTCCTGACTC+GGG | - | Chr3:88164714-88164733 | None:intergenic | 60.0% | |
| ! | GGCTTGGTCTCATACGGCGA+TGG | - | Chr3:88164729-88164748 | None:intergenic | 60.0% |
| !! | GTTCTCCTGACTCGGGCCAA+AGG | - | Chr3:88164707-88164726 | None:intergenic | 60.0% |
| AGAGGGGCGAGATCGATCGG+GGG | + | Chr3:88164886-88164905 | MsG0380016812.01.T01:CDS | 65.0% | |
| GAGAGGGGCGAGATCGATCG+GGG | + | Chr3:88164885-88164904 | MsG0380016812.01.T01:CDS | 65.0% | |
| GGAGAGGGGCGAGATCGATC+GGG | + | Chr3:88164884-88164903 | MsG0380016812.01.T01:CDS | 65.0% | |
| !! | CGGGGGAAGTGCTCCGAACT+GGG | + | Chr3:88164903-88164922 | MsG0380016812.01.T01:CDS | 65.0% |
| !! | CGTGGGCTGGGCGAAACAGA+TGG | + | Chr3:88164977-88164996 | MsG0380016812.01.T01:CDS | 65.0% |
| !! | GGGGGAAGTGCTCCGAACTG+GGG | + | Chr3:88164904-88164923 | MsG0380016812.01.T01:CDS | 65.0% |
| !! | TCGGGGGAAGTGCTCCGAAC+TGG | + | Chr3:88164902-88164921 | MsG0380016812.01.T01:CDS | 65.0% |
| CGAGCTCGGTAGCGTGGGCT+GGG | + | Chr3:88164965-88164984 | MsG0380016812.01.T01:CDS | 70.0% | |
| TCGAGCTCGGTAGCGTGGGC+TGG | + | Chr3:88164964-88164983 | MsG0380016812.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 88164663 | 88165025 | 88164663 | ID=MsG0380016812.01;Name=MsG0380016812.01 |
| Chr3 | mRNA | 88164663 | 88165025 | 88164663 | ID=MsG0380016812.01.T01;Parent=MsG0380016812.01;Name=MsG0380016812.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|120 |
| Chr3 | exon | 88164663 | 88165025 | 88164663 | ID=MsG0380016812.01.T01:exon:23767;Parent=MsG0380016812.01.T01 |
| Chr3 | CDS | 88164663 | 88165025 | 88164663 | ID=MsG0380016812.01.T01:cds;Parent=MsG0380016812.01.T01 |
| Gene Sequence |
| Protein sequence |
