AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009597.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009597.01.T01 MTR_2g067440 89.286 224 20 1 12 231 166 389 3.14e-144 408
MsG0280009597.01.T01 MTR_2g077990 89.189 222 20 1 14 231 187 408 3.21e-141 401
MsG0280009597.01.T01 MTR_2g078560 87.444 223 23 2 14 231 100 322 4.82e-140 395
MsG0280009597.01.T01 MTR_2g078610 87.838 222 23 1 14 231 187 408 6.06e-137 390
MsG0280009597.01.T01 MTR_2g078280 87.864 206 21 1 14 215 187 392 6.08e-128 367
MsG0280009597.01.T01 MTR_2g067450 75.893 224 20 2 12 231 119 312 3.87e-116 334
MsG0280009597.01.T01 MTR_2g077980 75.130 193 39 3 48 231 94 286 2.80e-96 283
MsG0280009597.01.T01 MTR_0147s0010 41.860 215 118 3 14 228 174 381 1.55e-43 150
MsG0280009597.01.T01 MTR_7g093370 31.944 216 119 4 15 228 181 370 8.21e-32 119
MsG0280009597.01.T01 MTR_2g099175 32.547 212 120 4 14 225 143 331 3.09e-31 117
MsG0280009597.01.T01 MTR_2g040000 31.674 221 121 4 15 231 138 332 4.19e-31 117
MsG0280009597.01.T01 MTR_1g054205 31.336 217 121 4 14 228 135 325 1.12e-30 115
MsG0280009597.01.T01 MTR_2g029730 33.488 215 115 5 14 226 135 323 3.80e-30 114
MsG0280009597.01.T01 MTR_2g437770 33.019 212 110 5 15 225 157 337 4.12e-30 114
MsG0280009597.01.T01 MTR_2g067350 78.788 66 12 1 168 231 12 77 2.65e-29 105
MsG0280009597.01.T01 MTR_4g133800 32.710 214 113 5 14 226 147 330 1.54e-28 110
MsG0280009597.01.T01 MTR_1g090760 30.374 214 124 2 15 228 141 329 2.31e-28 109
MsG0280009597.01.T01 MTR_4g125940 31.222 221 121 5 15 231 82 275 4.38e-28 107
MsG0280009597.01.T01 MTR_4g114210 32.258 217 117 5 16 228 129 319 8.70e-28 108
MsG0280009597.01.T01 MTR_2g008730 33.333 219 109 5 15 226 110 298 1.51e-27 107
MsG0280009597.01.T01 MTR_4g125940 32.127 221 119 5 15 231 137 330 2.35e-27 107
MsG0280009597.01.T01 MTR_4g029200 33.645 214 109 4 15 228 157 337 2.43e-27 107
MsG0280009597.01.T01 MTR_2g029740 31.308 214 119 4 15 226 140 327 3.44e-27 107
MsG0280009597.01.T01 MTR_3g466200 33.488 215 111 5 15 228 136 319 6.79e-27 105
MsG0280009597.01.T01 MTR_3g466180 32.243 214 112 4 15 228 142 322 1.85e-26 104
MsG0280009597.01.T01 MTR_4g029190 34.579 214 109 5 15 228 157 339 2.31e-26 104
MsG0280009597.01.T01 MTR_2g008710 32.877 219 110 5 15 226 134 322 2.37e-26 104
MsG0280009597.01.T01 MTR_2g029750 30.841 214 120 4 15 226 140 327 5.31e-26 103
MsG0280009597.01.T01 MTR_4g127670 29.907 214 124 3 14 227 134 321 7.46e-26 102
MsG0280009597.01.T01 MTR_7g072510 30.332 211 114 4 15 225 131 308 8.99e-26 102
MsG0280009597.01.T01 MTR_2g029560 30.698 215 121 4 14 226 134 322 1.36e-25 102
MsG0280009597.01.T01 MTR_3g094670 30.047 213 118 4 15 227 139 320 1.36e-25 102
MsG0280009597.01.T01 MTR_1g115900 34.434 212 106 7 15 225 138 317 1.66e-25 102
MsG0280009597.01.T01 MTR_5g021060 30.233 215 123 4 15 228 141 329 2.11e-25 102
MsG0280009597.01.T01 MTR_2g029880 30.986 213 109 4 16 226 9 185 2.37e-25 99.4
MsG0280009597.01.T01 MTR_1g101830 31.280 211 116 5 15 225 137 318 3.79e-25 100
MsG0280009597.01.T01 MTR_2g029800 29.245 212 122 4 16 225 141 326 3.89e-25 101
MsG0280009597.01.T01 MTR_7g086870 31.776 214 107 5 14 225 131 307 8.21e-25 100
MsG0280009597.01.T01 MTR_2g029815 30.516 213 118 5 16 225 141 326 8.88e-25 100
MsG0280009597.01.T01 MTR_2g029820 30.189 212 120 4 16 225 141 326 1.48e-24 100
MsG0280009597.01.T01 MTR_3g092990 30.734 218 125 4 14 231 125 316 1.51e-24 99.4
MsG0280009597.01.T01 MTR_1g086490 32.719 217 121 4 14 226 142 337 1.58e-24 99.8
MsG0280009597.01.T01 MTR_3g092990 30.734 218 125 4 14 231 136 327 3.46e-24 98.6
MsG0280009597.01.T01 MTR_3g094650 30.986 213 116 4 15 227 139 320 3.79e-24 98.2
MsG0280009597.01.T01 MTR_2g029830 29.108 213 123 4 16 226 72 258 4.69e-24 97.4
MsG0280009597.01.T01 MTR_1g086320 32.394 213 120 5 14 226 142 330 5.38e-24 98.2
MsG0280009597.01.T01 MTR_5g017860 32.243 214 119 5 12 225 135 322 5.58e-24 97.8
MsG0280009597.01.T01 MTR_1g066380 27.358 212 124 4 15 225 69 251 6.61e-24 96.3
MsG0280009597.01.T01 MTR_2g029850 31.163 215 120 4 14 226 139 327 6.68e-24 98.2
MsG0280009597.01.T01 MTR_2g028980 29.577 213 122 4 16 226 141 327 7.29e-24 98.2
MsG0280009597.01.T01 MTR_5g074970 32.243 214 114 6 15 227 141 324 8.84e-24 97.4
MsG0280009597.01.T01 MTR_8g062780 28.910 211 125 2 15 225 135 320 9.03e-24 97.4
MsG0280009597.01.T01 MTR_4g029170 31.481 216 110 5 15 227 139 319 9.22e-24 97.4
MsG0280009597.01.T01 MTR_2g088770 30.047 213 123 3 15 226 135 322 1.06e-23 97.1
MsG0280009597.01.T01 MTR_8g076820 30.516 213 121 5 14 225 140 326 1.09e-23 97.4
MsG0280009597.01.T01 MTR_2g029830 29.245 212 122 4 16 225 140 325 1.26e-23 97.4
MsG0280009597.01.T01 MTR_6g043280 29.907 214 116 5 12 225 149 328 1.29e-23 97.1
MsG0280009597.01.T01 MTR_1g066380 27.358 212 124 4 15 225 137 319 2.00e-23 96.3
MsG0280009597.01.T01 MTR_4g087965 30.841 214 122 4 14 226 155 343 2.45e-23 96.7
MsG0280009597.01.T01 MTR_5g016010 27.907 215 129 3 14 228 140 328 2.61e-23 96.3
MsG0280009597.01.T01 MTR_3g094630 30.516 213 117 4 15 227 139 320 3.23e-23 95.9
MsG0280009597.01.T01 MTR_1g066640 27.830 212 123 4 15 225 137 319 4.05e-23 95.5
MsG0280009597.01.T01 MTR_5g033470 29.167 216 127 5 12 225 182 373 6.28e-23 95.9
MsG0280009597.01.T01 MTR_1g077000 29.493 217 117 6 12 228 146 326 8.67e-23 94.7
MsG0280009597.01.T01 MTR_4g029180 30.841 214 114 5 15 227 139 319 8.77e-23 94.7
MsG0280009597.01.T01 MTR_1g038680 31.050 219 126 4 8 226 136 329 1.01e-22 94.7
MsG0280009597.01.T01 MTR_5g017850 29.907 214 124 4 12 225 135 322 1.13e-22 94.4
MsG0280009597.01.T01 MTR_7g086820 31.455 213 110 8 15 225 130 308 1.18e-22 94.4
MsG0280009597.01.T01 MTR_7g072480 29.858 211 115 4 15 225 135 312 1.44e-22 94.0
MsG0280009597.01.T01 MTR_2g029860 28.837 215 125 4 16 228 141 329 1.46e-22 94.4
MsG0280009597.01.T01 MTR_7g029030 29.907 214 120 5 15 228 136 319 1.47e-22 94.0
MsG0280009597.01.T01 MTR_2g084110 30.876 217 114 6 15 230 136 317 2.72e-22 93.2
MsG0280009597.01.T01 MTR_2g084090 31.019 216 116 7 15 230 136 318 2.93e-22 93.2
MsG0280009597.01.T01 MTR_2g084080 29.767 215 117 5 15 229 136 316 3.10e-22 93.2
MsG0280009597.01.T01 MTR_1g066710 27.962 211 124 4 15 225 137 319 4.27e-22 92.8
MsG0280009597.01.T01 MTR_7g026990 33.175 211 107 5 15 225 142 318 7.43e-22 92.0
MsG0280009597.01.T01 MTR_5g074860 30.734 218 113 6 15 228 141 324 1.37e-21 91.3
MsG0280009597.01.T01 MTR_0155s0040 29.439 214 115 5 15 228 137 314 1.85e-21 90.9
MsG0280009597.01.T01 MTR_4g031140 29.954 217 122 4 15 231 139 325 2.04e-21 91.3
MsG0280009597.01.T01 MTR_2g067310 77.778 54 12 0 168 221 8 61 2.38e-21 89.4
MsG0280009597.01.T01 MTR_6g043460 30.986 213 113 5 13 225 146 324 2.69e-21 90.9
MsG0280009597.01.T01 MTR_3g072190 29.907 214 117 4 15 228 70 250 3.42e-21 89.0
MsG0280009597.01.T01 MTR_0286s0010 30.841 214 121 6 15 228 147 333 3.52e-21 90.5
MsG0280009597.01.T01 MTR_6g008075 30.841 214 121 6 15 228 147 333 3.52e-21 90.5
MsG0280009597.01.T01 MTR_7g107520 29.333 225 132 4 2 226 120 317 9.82e-21 89.0
MsG0280009597.01.T01 MTR_4g132110 28.241 216 123 6 13 226 145 330 1.02e-20 89.4
MsG0280009597.01.T01 MTR_1g009750 27.315 216 131 3 13 228 121 310 1.32e-20 88.6
MsG0280009597.01.T01 MTR_3g072190 29.907 214 117 4 15 228 137 317 1.35e-20 88.6
MsG0280009597.01.T01 MTR_4g132110 28.037 214 122 6 15 226 142 325 1.60e-20 88.6
MsG0280009597.01.T01 MTR_2g084010 29.954 217 117 6 15 230 136 318 1.62e-20 88.6
MsG0280009597.01.T01 MTR_1g009750 27.570 214 129 3 15 228 140 327 1.77e-20 88.6
MsG0280009597.01.T01 MTR_4g030890 29.907 214 115 5 15 225 211 392 2.33e-20 89.0
MsG0280009597.01.T01 MTR_6g027440 31.905 210 112 5 16 225 132 310 2.44e-20 87.8
MsG0280009597.01.T01 MTR_2g029910 29.577 213 121 5 16 225 142 328 3.48e-20 87.8
MsG0280009597.01.T01 MTR_6g043240 28.774 212 117 5 15 226 151 328 3.81e-20 87.8
MsG0280009597.01.T01 MTR_4g046713 28.169 213 126 4 15 226 137 323 4.38e-20 87.4
MsG0280009597.01.T01 MTR_5g022870 29.646 226 122 7 3 228 127 315 6.21e-20 86.7
MsG0280009597.01.T01 MTR_1g066680 26.887 212 125 5 15 225 136 318 1.25e-19 86.3
MsG0280009597.01.T01 MTR_1g025980 30.806 211 116 5 15 225 134 314 1.62e-19 85.5
MsG0280009597.01.T01 MTR_5g083860 28.436 211 120 4 15 225 142 321 2.02e-19 85.5
MsG0280009597.01.T01 MTR_4g083710 28.837 215 123 5 15 228 138 323 2.33e-19 85.5
MsG0280009597.01.T01 MTR_1g022970 31.897 232 125 4 4 228 141 346 4.41e-19 84.7
MsG0280009597.01.T01 MTR_2g084020 29.167 216 118 7 15 229 136 317 1.28e-18 83.2
MsG0280009597.01.T01 MTR_3g467600 27.982 218 120 6 15 228 139 323 1.60e-18 83.2
MsG0280009597.01.T01 MTR_2g084000 28.241 216 120 6 15 229 137 318 1.89e-18 82.8
MsG0280009597.01.T01 MTR_5g074740 28.169 213 117 5 18 228 142 320 5.45e-18 81.6
MsG0280009597.01.T01 MTR_5g074760 27.700 213 118 5 18 228 142 320 7.81e-18 81.3
MsG0280009597.01.T01 MTR_5g049280 27.477 222 129 8 15 231 144 338 2.97e-17 79.7
MsG0280009597.01.T01 MTR_4g122640 27.830 212 124 5 15 225 149 332 2.04e-16 77.4
MsG0280009597.01.T01 MTR_4g095450 26.512 215 127 4 14 228 140 323 4.01e-16 76.3
MsG0280009597.01.T01 MTR_8g075100 28.372 215 122 8 15 229 136 318 4.77e-16 76.3
MsG0280009597.01.T01 MTR_2g008160 28.372 215 123 5 16 226 133 320 5.34e-16 75.9
MsG0280009597.01.T01 MTR_5g074710 26.761 213 120 5 18 228 142 320 1.00e-15 75.1
MsG0280009597.01.T01 MTR_3g072210 28.372 215 119 6 15 227 55 236 1.31e-15 73.6
MsG0280009597.01.T01 MTR_4g074995 26.977 215 114 6 15 228 135 307 4.87e-15 73.2
MsG0280009597.01.T01 MTR_0147s0040 45.783 83 44 1 146 228 1 82 2.85e-14 66.2
MsG0280009597.01.T01 MTR_4g074990 25.701 214 115 5 15 228 135 304 8.31e-13 66.6
MsG0280009597.01.T01 MTR_5g058120 27.273 187 97 4 40 225 7 155 1.89e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009597.01.T01 AT4G33870 40.889 225 120 5 14 227 177 399 2.16e-45 156
MsG0280009597.01.T01 AT2G38380 34.579 214 111 5 15 225 142 329 1.11e-31 119
MsG0280009597.01.T01 AT1G49570 36.150 213 109 6 15 225 159 346 1.52e-31 118
MsG0280009597.01.T01 AT4G36430 32.258 217 120 4 15 230 141 331 4.09e-31 117
MsG0280009597.01.T01 AT5G06720 33.180 217 117 5 14 228 142 332 1.37e-30 115
MsG0280009597.01.T01 AT2G38390 34.112 214 112 5 15 225 142 329 2.38e-30 115
MsG0280009597.01.T01 AT3G03670 31.308 214 121 3 12 225 130 317 9.27e-30 113
MsG0280009597.01.T01 AT5G24070 31.132 212 123 5 14 225 143 331 1.22e-29 113
MsG0280009597.01.T01 AT4G08770 34.234 222 115 5 14 231 134 328 9.20e-29 111
MsG0280009597.01.T01 AT5G17820 30.233 215 118 4 14 228 130 312 1.57e-28 110
MsG0280009597.01.T01 AT2G43480 29.767 215 128 4 14 228 143 334 1.66e-28 110
MsG0280009597.01.T01 AT4G08780 33.333 222 117 5 14 231 134 328 3.46e-28 109
MsG0280009597.01.T01 AT3G50990 30.233 215 123 3 15 228 152 340 7.67e-28 108
MsG0280009597.01.T01 AT5G17820 30.233 215 118 4 14 228 183 365 9.08e-28 108
MsG0280009597.01.T01 AT4G30170 30.374 214 123 3 15 228 137 324 1.31e-27 107
MsG0280009597.01.T01 AT2G41480 32.877 219 113 5 16 228 137 327 6.58e-27 105
MsG0280009597.01.T01 AT5G64120 33.178 214 113 5 16 228 143 327 2.27e-26 104
MsG0280009597.01.T01 AT2G35380 33.178 214 117 8 15 225 53 243 2.95e-26 102
MsG0280009597.01.T01 AT5G06730 29.907 214 122 4 14 225 143 330 1.03e-25 103
MsG0280009597.01.T01 AT1G30870 31.336 217 118 4 13 227 156 343 1.25e-25 102
MsG0280009597.01.T01 AT2G18150 28.899 218 128 4 15 231 147 338 1.33e-25 102
MsG0280009597.01.T01 AT2G35380 33.178 214 117 8 15 225 141 331 1.73e-25 102
MsG0280009597.01.T01 AT3G49110 34.685 222 114 6 14 231 143 337 3.69e-25 101
MsG0280009597.01.T01 AT2G18980 29.439 214 125 3 15 228 135 322 5.05e-25 100
MsG0280009597.01.T01 AT5G19880 29.358 218 125 5 14 228 137 328 5.81e-25 100
MsG0280009597.01.T01 AT5G15180 30.876 217 119 4 16 230 142 329 1.95e-24 99.4
MsG0280009597.01.T01 AT4G37530 29.717 212 123 3 14 225 140 325 2.91e-24 99.0
MsG0280009597.01.T01 AT3G49120 34.234 222 115 6 14 231 142 336 3.30e-24 99.0
MsG0280009597.01.T01 AT3G32980 33.784 222 116 6 14 231 141 335 4.70e-24 98.6
MsG0280009597.01.T01 AT1G05240 28.972 214 124 3 15 226 135 322 5.47e-24 98.2
MsG0280009597.01.T01 AT1G05250 28.972 214 124 3 15 226 135 322 5.47e-24 98.2
MsG0280009597.01.T01 AT3G21770 31.925 213 119 3 15 226 138 325 5.79e-24 98.2
MsG0280009597.01.T01 AT1G05260 31.925 213 119 3 15 226 135 322 1.73e-23 96.7
MsG0280009597.01.T01 AT3G01190 30.660 212 115 4 16 225 136 317 1.92e-23 96.7
MsG0280009597.01.T01 AT5G58390 31.776 214 114 5 15 227 132 314 3.44e-23 95.9
MsG0280009597.01.T01 AT5G51890 29.858 211 118 5 15 224 136 317 4.46e-23 95.5
MsG0280009597.01.T01 AT5G66390 28.372 215 127 3 15 228 144 332 5.77e-23 95.5
MsG0280009597.01.T01 AT2G18140 28.440 218 129 4 15 231 146 337 7.31e-23 95.1
MsG0280009597.01.T01 AT2G22420 28.910 211 121 3 15 225 134 315 1.36e-22 94.4
MsG0280009597.01.T01 AT1G14550 31.776 214 112 6 15 226 137 318 1.48e-22 94.0
MsG0280009597.01.T01 AT5G40150 33.175 211 111 6 15 225 144 324 2.66e-22 93.6
MsG0280009597.01.T01 AT4G37520 27.830 212 127 3 14 225 137 322 2.95e-22 93.2
MsG0280009597.01.T01 AT4G37520 27.830 212 127 3 14 225 140 325 3.17e-22 93.2
MsG0280009597.01.T01 AT4G21960 29.439 214 119 6 15 226 141 324 3.27e-22 93.2
MsG0280009597.01.T01 AT1G68850 31.481 216 118 7 15 225 141 331 5.97e-22 92.8
MsG0280009597.01.T01 AT5G19890 29.302 215 119 6 15 225 138 323 8.81e-22 92.0
MsG0280009597.01.T01 AT3G28200 31.754 211 113 5 15 225 133 312 9.45e-22 91.7
MsG0280009597.01.T01 AT5G42180 30.415 217 115 7 15 228 133 316 1.16e-21 91.7
MsG0280009597.01.T01 AT4G25980 31.163 215 118 4 16 226 135 323 1.60e-21 91.3
MsG0280009597.01.T01 AT5G05340 27.700 213 123 4 15 227 141 322 6.64e-21 89.7
MsG0280009597.01.T01 AT4G11290 30.660 212 123 3 15 226 136 323 7.45e-21 89.4
MsG0280009597.01.T01 AT3G17070 28.704 216 126 6 15 228 86 275 1.55e-20 87.8
MsG0280009597.01.T01 AT1G44970 32.710 214 114 4 15 225 156 342 1.56e-20 89.0
MsG0280009597.01.T01 AT2G37130 27.805 205 120 4 21 225 116 292 1.58e-20 88.2
MsG0280009597.01.T01 AT2G37130 27.805 205 120 4 21 225 147 323 1.91e-20 88.6
MsG0280009597.01.T01 AT3G17070 28.704 216 126 6 15 228 149 338 3.54e-20 87.8
MsG0280009597.01.T01 AT5G64110 28.571 217 126 4 14 228 140 329 4.00e-20 87.4
MsG0280009597.01.T01 AT4G33420 29.907 214 113 6 12 225 145 321 5.50e-20 87.0
MsG0280009597.01.T01 AT4G33420 29.907 214 113 6 12 225 192 368 7.22e-20 87.4
MsG0280009597.01.T01 AT5G39580 30.374 214 120 7 16 228 133 318 2.15e-19 85.5
MsG0280009597.01.T01 AT2G34060 28.638 213 126 4 14 225 155 342 3.32e-19 85.1
MsG0280009597.01.T01 AT2G39040 30.556 216 123 6 17 230 160 350 4.21e-19 85.1
MsG0280009597.01.T01 AT5G14130 28.774 212 124 3 15 226 143 327 5.40e-19 84.3
MsG0280009597.01.T01 AT5G22410 27.700 213 126 6 14 225 136 321 5.56e-19 84.3
MsG0280009597.01.T01 AT5G14130 28.774 212 124 3 15 226 184 368 7.79e-19 84.3
MsG0280009597.01.T01 AT1G14540 30.516 213 115 5 15 226 132 312 9.57e-19 83.6
MsG0280009597.01.T01 AT5G58400 29.439 214 119 5 15 227 141 323 1.10e-18 83.6
MsG0280009597.01.T01 AT1G24110 27.014 211 128 3 15 225 134 318 3.61e-18 82.0
MsG0280009597.01.T01 AT4G17690 27.488 211 123 5 15 225 138 318 4.36e-18 82.0
MsG0280009597.01.T01 AT5G47000 26.540 211 126 4 15 225 145 326 5.74e-18 81.6
MsG0280009597.01.T01 AT4G37530 29.762 168 93 2 14 181 140 282 2.38e-17 79.3
MsG0280009597.01.T01 AT5G64100 26.728 217 126 5 15 228 144 330 4.76e-17 79.0
MsG0280009597.01.T01 AT3G49960 24.528 212 134 3 14 225 140 325 4.72e-16 76.3
MsG0280009597.01.T01 AT2G24800 26.168 214 135 4 15 228 138 328 4.21e-15 73.6
MsG0280009597.01.T01 AT1G34510 25.352 213 120 6 14 225 131 305 5.68e-14 70.1
MsG0280009597.01.T01 AT5G67400 24.286 210 133 3 16 225 142 325 4.82e-13 67.8
MsG0280009597.01.T01 AT4G31760 26.512 215 134 4 14 228 135 325 2.36e-11 62.8

Find 51 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGAGTGATGATCATTATT+TGG 0.228393 2:-54943807 None:intergenic
CTGCACCTCTTCAATCTTAG+AGG 0.300466 2:+54943336 MsG0280009597.01.T01:CDS
GAATTAGATCAGTTGCTTCC+TGG 0.320680 2:-54943284 None:intergenic
CATGGCAATGTCGATAAATA+AGG 0.324278 2:-54943198 None:intergenic
TCTCGTCTGCCTGTTAAAAC+TGG 0.372361 2:-54943243 None:intergenic
CTGTACGACTTTCAAGGAAC+AGG 0.416515 2:+54943666 MsG0280009597.01.T01:CDS
GTTTGCTGACCAACAGCTAA+TGG 0.417017 2:+54943923 MsG0280009597.01.T01:CDS
CAGAGTTTGTTGAGAGGAAG+AGG 0.433418 2:+54943894 MsG0280009597.01.T01:CDS
CGCTTAAGAGCTTACGAGAC+GGG 0.438043 2:-54944090 None:intergenic
TCGCAAGGGTTATGATGAAA+TGG 0.438179 2:+54944015 MsG0280009597.01.T01:CDS
TGAAGAGAAGACAGCCAGAT+TGG 0.447979 2:+54943947 MsG0280009597.01.T01:CDS
CAAAGGCTGTACGACTTTCA+AGG 0.449968 2:+54943660 MsG0280009597.01.T01:CDS
TTTATCGACATTGCCATGGC+AGG 0.451626 2:+54943203 MsG0280009597.01.T01:CDS
CAGATGACGGATCAACCTTT+CGG 0.458746 2:+54943985 MsG0280009597.01.T01:CDS
CGGGAAACAATCAGCCTCCT+TGG 0.466618 2:+54943369 MsG0280009597.01.T01:CDS
CCTGTTAAAACTGGATAGAA+AGG 0.468106 2:-54943234 None:intergenic
AGAGTTTGTTGAGAGGAAGA+GGG 0.471781 2:+54943895 MsG0280009597.01.T01:CDS
TCTGCTTATGCTTCAGATGA+CGG 0.489775 2:+54943972 MsG0280009597.01.T01:CDS
TACATGCAAGCATTGTCATC+TGG 0.489841 2:+54943846 MsG0280009597.01.T01:CDS
TCTCTTCAGCCATTAGCTGT+TGG 0.493900 2:-54943932 None:intergenic
AGCATAAGCAGAAACCAATC+TGG 0.500027 2:-54943961 None:intergenic
ATCTTAGAGGATTCAATGCA+CGG 0.504217 2:+54943349 MsG0280009597.01.T01:CDS
TTTGACTGGAAATCAAGGTC+AGG 0.507215 2:+54944052 MsG0280009597.01.T01:CDS
TCGAGAGGTATACTCGGGTC+TGG 0.511806 2:-54943693 None:intergenic
AAGAATAATGTCAGCAGCAA+CGG 0.514444 2:+54943756 MsG0280009597.01.T01:CDS
ACATTGCCATGGCAGGGTGG+TGG 0.516409 2:+54943210 MsG0280009597.01.T01:CDS
GATTTCCAGTCAAAACATCA+AGG 0.539193 2:-54944043 None:intergenic
GTAATGTTATCGTCAGGCCT+TGG 0.540087 2:-54943306 None:intergenic
TCTGAAGGTGCACACTACAT+TGG 0.541527 2:+54943612 MsG0280009597.01.T01:intron
GTGCGCGTAATGTTATCGTC+AGG 0.553348 2:-54943312 None:intergenic
TGACGGATCAACCTTTCGGA+TGG 0.559114 2:+54943989 MsG0280009597.01.T01:CDS
AGGAAATCGAGAGGTATACT+CGG 0.576740 2:-54943699 None:intergenic
CCTTTCGGATGGATTTCGCA+AGG 0.582493 2:+54944000 MsG0280009597.01.T01:CDS
CTTATTTATCGACATTGCCA+TGG 0.585488 2:+54943199 MsG0280009597.01.T01:CDS
TCTTAGAGGATTCAATGCAC+GGG 0.590163 2:+54943350 MsG0280009597.01.T01:CDS
GTTGCGATTTCATTCAGCAA+AGG 0.590568 2:+54943643 MsG0280009597.01.T01:CDS
CTTTCGGATGGATTTCGCAA+GGG 0.591202 2:+54944001 MsG0280009597.01.T01:CDS
TTATCGACATTGCCATGGCA+GGG 0.604928 2:+54943204 MsG0280009597.01.T01:CDS
AAAGGTCCACCACCCTGCCA+TGG 0.610798 2:-54943216 None:intergenic
TGAATCCTCTAAGATTGAAG+AGG 0.616668 2:-54943341 None:intergenic
GATCATCACTCTGATAACAA+AGG 0.617209 2:+54943816 MsG0280009597.01.T01:CDS
AAGCAACTGATCTAATTCCA+AGG 0.625494 2:+54943289 MsG0280009597.01.T01:CDS
TCGATTTCCTCAGTCAGATG+AGG 0.631033 2:+54943712 MsG0280009597.01.T01:CDS
GGAAATCGAGAGGTATACTC+GGG 0.660785 2:-54943698 None:intergenic
TACTATCAGAGTTTGTTGAG+AGG 0.661163 2:+54943888 MsG0280009597.01.T01:CDS
TCGCTTAAGAGCTTACGAGA+CGG 0.666615 2:-54944091 None:intergenic
CCTTGCGAAATCCATCCGAA+AGG 0.669038 2:-54944000 None:intergenic
TGTACGACTTTCAAGGAACA+GGG 0.687437 2:+54943667 MsG0280009597.01.T01:CDS
TCGACATTGCCATGGCAGGG+TGG 0.699268 2:+54943207 MsG0280009597.01.T01:CDS
ATCTGACTGAGGAAATCGAG+AGG 0.699340 2:-54943708 None:intergenic
TGTAAAGTAACTTACCAAGG+AGG 0.719696 2:-54943383 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGATTATAATACGAAAA+AGG + Chr2:54943415-54943434 MsG0280009597.01.T01:intron 15.0%
!!! ACATTATTCTTATTTTTGTC+AGG - Chr2:54943747-54943766 None:intergenic 20.0%
! GATCTCATTAGTCATATTAT+AGG - Chr2:54943575-54943594 None:intergenic 25.0%
AAGTGTAAAGTAACTTACCA+AGG - Chr2:54943389-54943408 None:intergenic 30.0%
ATATGACTAATGAGATCTGT+TGG + Chr2:54943578-54943597 MsG0280009597.01.T01:intron 30.0%
TACGAAAAAGGTTTGTATCT+TGG + Chr2:54943427-54943446 MsG0280009597.01.T01:intron 30.0%
TCAGAGTGATGATCATTATT+TGG - Chr2:54943810-54943829 None:intergenic 30.0%
! TTGGTTTCTTGATAATCTGA+AGG + Chr2:54943597-54943616 MsG0280009597.01.T01:intron 30.0%
AAGAATAATGTCAGCAGCAA+CGG + Chr2:54943756-54943775 MsG0280009597.01.T01:CDS 35.0%
AAGCAACTGATCTAATTCCA+AGG + Chr2:54943289-54943308 MsG0280009597.01.T01:CDS 35.0%
ATCTTAGAGGATTCAATGCA+CGG + Chr2:54943349-54943368 MsG0280009597.01.T01:CDS 35.0%
CATGGCAATGTCGATAAATA+AGG - Chr2:54943201-54943220 None:intergenic 35.0%
CCTGTTAAAACTGGATAGAA+AGG - Chr2:54943237-54943256 None:intergenic 35.0%
CTTATTTATCGACATTGCCA+TGG + Chr2:54943199-54943218 MsG0280009597.01.T01:CDS 35.0%
GATCATCACTCTGATAACAA+AGG + Chr2:54943816-54943835 MsG0280009597.01.T01:CDS 35.0%
GATTTCCAGTCAAAACATCA+AGG - Chr2:54944046-54944065 None:intergenic 35.0%
GCACACTACATTGGAAAAAT+CGG + Chr2:54943621-54943640 MsG0280009597.01.T01:CDS 35.0%
TGTAAAGTAACTTACCAAGG+AGG - Chr2:54943386-54943405 None:intergenic 35.0%
!! CCTTTCTATCCAGTTTTAAC+AGG + Chr2:54943234-54943253 MsG0280009597.01.T01:CDS 35.0%
!! TACTATCAGAGTTTGTTGAG+AGG + Chr2:54943888-54943907 MsG0280009597.01.T01:CDS 35.0%
!! TGAATCCTCTAAGATTGAAG+AGG - Chr2:54943344-54943363 None:intergenic 35.0%
!!! GATGTTTTGACTGGAAATCA+AGG + Chr2:54944047-54944066 MsG0280009597.01.T01:CDS 35.0%
!!! TCTAACCTTGATGTTTTGAC+TGG + Chr2:54944038-54944057 MsG0280009597.01.T01:CDS 35.0%
AGCATAAGCAGAAACCAATC+TGG - Chr2:54943964-54943983 None:intergenic 40.0%
GAATTAGATCAGTTGCTTCC+TGG - Chr2:54943287-54943306 None:intergenic 40.0%
GCAATAACACGATTGCAATG+CGG - Chr2:54943453-54943472 None:intergenic 40.0%
GTTGCGATTTCATTCAGCAA+AGG + Chr2:54943643-54943662 MsG0280009597.01.T01:CDS 40.0%
TCGCAAGGGTTATGATGAAA+TGG + Chr2:54944015-54944034 MsG0280009597.01.T01:CDS 40.0%
TCTGCTTATGCTTCAGATGA+CGG + Chr2:54943972-54943991 MsG0280009597.01.T01:CDS 40.0%
TCTTAGAGGATTCAATGCAC+GGG + Chr2:54943350-54943369 MsG0280009597.01.T01:CDS 40.0%
TGTACGACTTTCAAGGAACA+GGG + Chr2:54943667-54943686 MsG0280009597.01.T01:CDS 40.0%
TTTGACTGGAAATCAAGGTC+AGG + Chr2:54944052-54944071 MsG0280009597.01.T01:CDS 40.0%
! AGGAAATCGAGAGGTATACT+CGG - Chr2:54943702-54943721 None:intergenic 40.0%
! TACATGCAAGCATTGTCATC+TGG + Chr2:54943846-54943865 MsG0280009597.01.T01:CDS 40.0%
!! AGAGTTTGTTGAGAGGAAGA+GGG + Chr2:54943895-54943914 MsG0280009597.01.T01:CDS 40.0%
ACCACAGTTGAGATTGCATC+AGG - Chr2:54943492-54943511 None:intergenic 45.0%
ACCTGATGCAATCTCAACTG+TGG + Chr2:54943488-54943507 MsG0280009597.01.T01:intron 45.0%
ATCTGACTGAGGAAATCGAG+AGG - Chr2:54943711-54943730 None:intergenic 45.0%
CAAAGGCTGTACGACTTTCA+AGG + Chr2:54943660-54943679 MsG0280009597.01.T01:CDS 45.0%
CAATTTCAGCTGCGACATCA+TGG - Chr2:54943532-54943551 None:intergenic 45.0%
CTGCACCTCTTCAATCTTAG+AGG + Chr2:54943336-54943355 MsG0280009597.01.T01:CDS 45.0%
CTGTACGACTTTCAAGGAAC+AGG + Chr2:54943666-54943685 MsG0280009597.01.T01:CDS 45.0%
CTTCCTGGAAAAACGATTGC+AGG - Chr2:54943272-54943291 None:intergenic 45.0%
GTAATGTTATCGTCAGGCCT+TGG - Chr2:54943309-54943328 None:intergenic 45.0%
TCGATTTCCTCAGTCAGATG+AGG + Chr2:54943712-54943731 MsG0280009597.01.T01:CDS 45.0%
TCTCGTCTGCCTGTTAAAAC+TGG - Chr2:54943246-54943265 None:intergenic 45.0%
TCTCTTCAGCCATTAGCTGT+TGG - Chr2:54943935-54943954 None:intergenic 45.0%
TGAAGAGAAGACAGCCAGAT+TGG + Chr2:54943947-54943966 MsG0280009597.01.T01:CDS 45.0%
TTATCGACATTGCCATGGCA+GGG + Chr2:54943204-54943223 MsG0280009597.01.T01:CDS 45.0%
TTTATCGACATTGCCATGGC+AGG + Chr2:54943203-54943222 MsG0280009597.01.T01:CDS 45.0%
! AGTTTTGCCTCATCTGACTG+AGG - Chr2:54943722-54943741 None:intergenic 45.0%
! CTTTCGGATGGATTTCGCAA+GGG + Chr2:54944001-54944020 MsG0280009597.01.T01:CDS 45.0%
! GGAAATCGAGAGGTATACTC+GGG - Chr2:54943701-54943720 None:intergenic 45.0%
! GTTTGCTGACCAACAGCTAA+TGG + Chr2:54943923-54943942 MsG0280009597.01.T01:CDS 45.0%
! TCTGAAGGTGCACACTACAT+TGG + Chr2:54943612-54943631 MsG0280009597.01.T01:intron 45.0%
!! CAGAGTTTGTTGAGAGGAAG+AGG + Chr2:54943894-54943913 MsG0280009597.01.T01:CDS 45.0%
!! CAGATGACGGATCAACCTTT+CGG + Chr2:54943985-54944004 MsG0280009597.01.T01:CDS 45.0%
!! TAGCCTGCAATCGTTTTTCC+AGG + Chr2:54943266-54943285 MsG0280009597.01.T01:CDS 45.0%
CCTTGCGAAATCCATCCGAA+AGG - Chr2:54944003-54944022 None:intergenic 50.0%
GTGCGCGTAATGTTATCGTC+AGG - Chr2:54943315-54943334 None:intergenic 50.0%
! CCTTTCGGATGGATTTCGCA+AGG + Chr2:54944000-54944019 MsG0280009597.01.T01:CDS 50.0%
! TGACGGATCAACCTTTCGGA+TGG + Chr2:54943989-54944008 MsG0280009597.01.T01:CDS 50.0%
CGGGAAACAATCAGCCTCCT+TGG + Chr2:54943369-54943388 MsG0280009597.01.T01:CDS 55.0%
! TCGAGAGGTATACTCGGGTC+TGG - Chr2:54943696-54943715 None:intergenic 55.0%
AAAGGTCCACCACCCTGCCA+TGG - Chr2:54943219-54943238 None:intergenic 60.0%
ACATTGCCATGGCAGGGTGG+TGG + Chr2:54943210-54943229 MsG0280009597.01.T01:CDS 60.0%
TCGACATTGCCATGGCAGGG+TGG + Chr2:54943207-54943226 MsG0280009597.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 54943186 54944109 54943186 ID=MsG0280009597.01;Name=MsG0280009597.01
Chr2 mRNA 54943186 54944109 54943186 ID=MsG0280009597.01.T01;Parent=MsG0280009597.01;Name=MsG0280009597.01.T01;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|1|1|2|0|231
Chr2 exon 54943186 54943390 54943186 ID=MsG0280009597.01.T01:exon:20677;Parent=MsG0280009597.01.T01
Chr2 exon 54943619 54944109 54943619 ID=MsG0280009597.01.T01:exon:20676;Parent=MsG0280009597.01.T01
Chr2 CDS 54943186 54943390 54943186 ID=MsG0280009597.01.T01:cds;Parent=MsG0280009597.01.T01
Chr2 CDS 54943619 54944109 54943619 ID=MsG0280009597.01.T01:cds;Parent=MsG0280009597.01.T01
Gene Sequence

>MsG0280009597.01.T01

TTGTCTGATTGTCCTTATTTATCGACATTGCCATGGCAGGGTGGTGGACCTTTCTATCCAGTTTTAACAGGCAGACGAGATAGCCTGCAATCGTTTTTCCAGGAAGCAACTGATCTAATTCCAAGGCCTGACGATAACATTACGCGCACACTGCACCTCTTCAATCTTAGAGGATTCAATGCACGGGAAACAATCAGCCTCCTTGGTGCACACTACATTGGAAAAATCGGTTGCGATTTCATTCAGCAAAGGCTGTACGACTTTCAAGGAACAGGGCAACCAGACCCGAGTATACCTCTCGATTTCCTCAGTCAGATGAGGCAAAACTGTCCTGACAAAAATAAGAATAATGTCAGCAGCAACGGCACGTTTTCGACATTTACAGCTTCAAAGCCAAATAATGATCATCACTCTGATAACAAAGGAATGTCATACATGCAAGCATTGTCATCTGGCGCATCTTTTGTCACTCACTACTATCAGAGTTTGTTGAGAGGAAGAGGGTTATTGTTTGCTGACCAACAGCTAATGGCTGAAGAGAAGACAGCCAGATTGGTTTCTGCTTATGCTTCAGATGACGGATCAACCTTTCGGATGGATTTCGCAAGGGTTATGATGAAATGGTCTAACCTTGATGTTTTGACTGGAAATCAAGGTCAGGTTCGTCTGAATTGCTCCCGTCTCGTAAGCTCTTAA
Protein sequence

>MsG0280009597.01.T01

LSDCPYLSTLPWQGGGPFYPVLTGRRDSLQSFFQEATDLIPRPDDNITRTLHLFNLRGFNARETISLLGAHYIGKIGCDFIQQRLYDFQGTGQPDPSIPLDFLSQMRQNCPDKNKNNVSSNGTFSTFTASKPNNDHHSDNKGMSYMQALSSGASFVTHYYQSLLRGRGLLFADQQLMAEEKTARLVSAYASDDGSTFRMDFARVMMKWSNLDVLTGNQGQVRLNCSRLVSS*