Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008206.01.T01 | XP_024631816.1 | 55.851 | 188 | 22 | 3 | 2 | 128 | 169 | 356 | 7.78E-55 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008206.01.T01 | A2Q1L7 | 55.851 | 188 | 22 | 3 | 2 | 128 | 217 | 404 | 1.74e-54 | 186 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280008206.01 | MsG0280008220.01 | 0.823057 | 1.699629e-53 | 1.447875e-50 |
| MsG0280008206.01 | MsG0380017286.01 | 0.802066 | 6.691910e-49 | 3.247078e-46 |
| MsG0280008206.01 | MsG0580024114.01 | 0.801798 | 7.594867e-49 | 3.660247e-46 |
| MsG0280008206.01 | MsG0580029758.01 | 0.811715 | 6.095666e-51 | 3.800797e-48 |
| MsG0280008206.01 | MsG0680031459.01 | 0.828934 | 6.820742e-55 | 6.877603e-52 |
| MsG0280008206.01 | MsG0780039875.01 | 0.805826 | 1.106683e-49 | 5.913690e-47 |
| MsG0280008206.01 | MsG0780040679.01 | 0.810448 | 1.146948e-50 | 6.915343e-48 |
| MsG0280008206.01 | MsG0880043288.01 | 0.803501 | 3.382689e-49 | 1.702702e-46 |
| MsG0280008206.01 | MsG0880046731.01 | 0.802203 | 6.270057e-49 | 3.052971e-46 |
| MsG0280008206.01 | MsG0880047425.01 | 0.806201 | 9.229955e-50 | 4.979697e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008206.01.T01 | MTR_2g036480 | 55.851 | 188 | 22 | 3 | 2 | 128 | 217 | 404 | 4.41e-58 | 186 |
| MsG0280008206.01.T01 | MTR_2g036460 | 46.635 | 208 | 51 | 3 | 2 | 149 | 269 | 476 | 1.27e-51 | 171 |
| MsG0280008206.01.T01 | MTR_2g036490 | 46.154 | 208 | 52 | 3 | 2 | 149 | 259 | 466 | 2.31e-49 | 164 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAAGCCTTCCACATTTC+TGG | 0.164271 | 2:+27185374 | None:intergenic |
| AGCCAAACAAAGTGCATTTC+AGG | 0.238346 | 2:-27184922 | MsG0280008206.01.T01:CDS |
| CCTTGTCAAGAAGAATATTT+TGG | 0.252969 | 2:+27185501 | None:intergenic |
| GAGAGCTGAGAGAATGTTAA+AGG | 0.275216 | 2:-27185160 | MsG0280008206.01.T01:CDS |
| ACAAAACCTTTGAGGGAATC+TGG | 0.304013 | 2:-27184951 | MsG0280008206.01.T01:CDS |
| GGCTCGATTTCCCTCATAAT+CGG | 0.307208 | 2:-27185046 | MsG0280008206.01.T01:CDS |
| GTACCGAAGGTAGCTGATAT+TGG | 0.335910 | 2:-27185473 | MsG0280008206.01.T01:CDS |
| AACCTGAAATGCACTTTGTT+TGG | 0.339205 | 2:+27184920 | None:intergenic |
| TTTCCCTCATAATCGGGTTT+CGG | 0.381697 | 2:-27185039 | MsG0280008206.01.T01:CDS |
| GCAATGAATACTCGCTTTGA+AGG | 0.383153 | 2:-27185416 | MsG0280008206.01.T01:CDS |
| AATGTTTGAGAATAATGAAT+TGG | 0.409015 | 2:-27185226 | MsG0280008206.01.T01:CDS |
| TTAGCCGAAACCCGATTATG+AGG | 0.427804 | 2:+27185035 | None:intergenic |
| GCTCGATTTCCCTCATAATC+GGG | 0.461935 | 2:-27185045 | MsG0280008206.01.T01:CDS |
| ATGGCATACAAAACCTTTGA+GGG | 0.500318 | 2:-27184958 | MsG0280008206.01.T01:CDS |
| CCATGATGAGGTAGCAGTAT+CGG | 0.507031 | 2:+27184977 | None:intergenic |
| CTGAAACCAGATTCCCTCAA+AGG | 0.528164 | 2:+27184945 | None:intergenic |
| CCAAAATATTCTTCTTGACA+AGG | 0.545366 | 2:-27185501 | MsG0280008206.01.T01:CDS |
| CCGATACTGCTACCTCATCA+TGG | 0.547827 | 2:-27184977 | MsG0280008206.01.T01:CDS |
| TAAAGAAAATTTGACGCAAG+AGG | 0.578657 | 2:-27185015 | MsG0280008206.01.T01:CDS |
| GAATGTTAAAGGTTGCTCAA+TGG | 0.578669 | 2:-27185149 | MsG0280008206.01.T01:intron |
| CACACCGTCACACACTAAGT+AGG | 0.582076 | 2:+27184891 | None:intergenic |
| CATGGCATACAAAACCTTTG+AGG | 0.585048 | 2:-27184959 | MsG0280008206.01.T01:CDS |
| TGACAAGGAATTTGTACCGA+AGG | 0.588835 | 2:-27185486 | MsG0280008206.01.T01:CDS |
| TAGCCGAAACCCGATTATGA+GGG | 0.592753 | 2:+27185036 | None:intergenic |
| AATCCAATATCAGCTACCTT+CGG | 0.599205 | 2:+27185470 | None:intergenic |
| GATATAGCAAATCACAAATG+TGG | 0.606433 | 2:-27185269 | MsG0280008206.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTTGAGAATAATGAAT+TGG | - | Chr2:27185191-27185210 | MsG0280008206.01.T01:CDS | 20.0% |
| !!! | AAATGTGATGTTTATAGTTT+TGG | - | Chr2:27185076-27185095 | MsG0280008206.01.T01:intron | 20.0% |
| !!! | ATTCTTCTATTTGAAATTGT+AGG | - | Chr2:27185100-27185119 | MsG0280008206.01.T01:intron | 20.0% |
| ! | ACATCACATTTATATGTTTC+CGG | + | Chr2:27185067-27185086 | None:intergenic | 25.0% |
| CCAAAATATTCTTCTTGACA+AGG | - | Chr2:27184916-27184935 | MsG0280008206.01.T01:CDS | 30.0% | |
| GATATAGCAAATCACAAATG+TGG | - | Chr2:27185148-27185167 | MsG0280008206.01.T01:intron | 30.0% | |
| TAAAGAAAATTTGACGCAAG+AGG | - | Chr2:27185402-27185421 | MsG0280008206.01.T01:CDS | 30.0% | |
| ! | CACAAATGTGGTTTTCAAAA+AGG | - | Chr2:27185160-27185179 | MsG0280008206.01.T01:CDS | 30.0% |
| ! | CCTTGTCAAGAAGAATATTT+TGG | + | Chr2:27184919-27184938 | None:intergenic | 30.0% |
| ! | GGGTGAAAAAGAAATTTTAC+AGG | - | Chr2:27185350-27185369 | MsG0280008206.01.T01:intron | 30.0% |
| AACCTGAAATGCACTTTGTT+TGG | + | Chr2:27185500-27185519 | None:intergenic | 35.0% | |
| AATCCAATATCAGCTACCTT+CGG | + | Chr2:27184950-27184969 | None:intergenic | 35.0% | |
| ACTGTTGTGAAAATGTTGGA+GGG | - | Chr2:27185330-27185349 | MsG0280008206.01.T01:intron | 35.0% | |
| ATGGCATACAAAACCTTTGA+GGG | - | Chr2:27185459-27185478 | MsG0280008206.01.T01:CDS | 35.0% | |
| GAATGTTAAAGGTTGCTCAA+TGG | - | Chr2:27185268-27185287 | MsG0280008206.01.T01:CDS | 35.0% | |
| TACTGTTGTGAAAATGTTGG+AGG | - | Chr2:27185329-27185348 | MsG0280008206.01.T01:intron | 35.0% | |
| TTCACAACAGTACTCATTAG+AGG | + | Chr2:27185321-27185340 | None:intergenic | 35.0% | |
| ! | GAGTACTGTTGTGAAAATGT+TGG | - | Chr2:27185326-27185345 | MsG0280008206.01.T01:intron | 35.0% |
| ! | GTGGTTTTCAAAAAGGACAT+GGG | - | Chr2:27185167-27185186 | MsG0280008206.01.T01:CDS | 35.0% |
| ! | GTTGCAATGCTTGAATTTTG+TGG | - | Chr2:27185213-27185232 | MsG0280008206.01.T01:CDS | 35.0% |
| ! | TGTGGTTTTCAAAAAGGACA+TGG | - | Chr2:27185166-27185185 | MsG0280008206.01.T01:CDS | 35.0% |
| AGAAATGTGGAAGGCTTATC+CGG | - | Chr2:27185045-27185064 | MsG0280008206.01.T01:CDS | 40.0% | |
| AGCCAAACAAAGTGCATTTC+AGG | - | Chr2:27185495-27185514 | MsG0280008206.01.T01:CDS | 40.0% | |
| CATGGCATACAAAACCTTTG+AGG | - | Chr2:27185458-27185477 | MsG0280008206.01.T01:CDS | 40.0% | |
| CTCATTAGAGGTCTATCATC+TGG | + | Chr2:27185309-27185328 | None:intergenic | 40.0% | |
| GAGAGCTGAGAGAATGTTAA+AGG | - | Chr2:27185257-27185276 | MsG0280008206.01.T01:CDS | 40.0% | |
| GCAATGAATACTCGCTTTGA+AGG | - | Chr2:27185001-27185020 | MsG0280008206.01.T01:CDS | 40.0% | |
| TGACAAGGAATTTGTACCGA+AGG | - | Chr2:27184931-27184950 | MsG0280008206.01.T01:CDS | 40.0% | |
| TTTCCCTCATAATCGGGTTT+CGG | - | Chr2:27185378-27185397 | MsG0280008206.01.T01:intron | 40.0% | |
| ! | ACAAAACCTTTGAGGGAATC+TGG | - | Chr2:27185466-27185485 | MsG0280008206.01.T01:CDS | 40.0% |
| !! | AGGTTTTGTATGCCATGATG+AGG | + | Chr2:27185455-27185474 | None:intergenic | 40.0% |
| CCATGATGAGGTAGCAGTAT+CGG | + | Chr2:27185443-27185462 | None:intergenic | 45.0% | |
| CTGAAACCAGATTCCCTCAA+AGG | + | Chr2:27185475-27185494 | None:intergenic | 45.0% | |
| CTTATGCAGCACCAGAAATG+TGG | - | Chr2:27185032-27185051 | MsG0280008206.01.T01:CDS | 45.0% | |
| GCTCGATTTCCCTCATAATC+GGG | - | Chr2:27185372-27185391 | MsG0280008206.01.T01:intron | 45.0% | |
| GGATAAGCCTTCCACATTTC+TGG | + | Chr2:27185046-27185065 | None:intergenic | 45.0% | |
| GGCTCGATTTCCCTCATAAT+CGG | - | Chr2:27185371-27185390 | MsG0280008206.01.T01:intron | 45.0% | |
| GTACCGAAGGTAGCTGATAT+TGG | - | Chr2:27184944-27184963 | MsG0280008206.01.T01:CDS | 45.0% | |
| TAGCCGAAACCCGATTATGA+GGG | + | Chr2:27185384-27185403 | None:intergenic | 45.0% | |
| TTAGCCGAAACCCGATTATG+AGG | + | Chr2:27185385-27185404 | None:intergenic | 45.0% | |
| CCGATACTGCTACCTCATCA+TGG | - | Chr2:27185440-27185459 | MsG0280008206.01.T01:CDS | 50.0% | |
| TGCAGCACCAGAAATGTGGA+AGG | - | Chr2:27185036-27185055 | MsG0280008206.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 27184905 | 27185534 | 27184905 | ID=MsG0280008206.01;Name=MsG0280008206.01 |
| Chr2 | mRNA | 27184905 | 27185534 | 27184905 | ID=MsG0280008206.01.T01;Parent=MsG0280008206.01;Name=MsG0280008206.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|3|0|149 |
| Chr2 | exon | 27185389 | 27185534 | 27185389 | ID=MsG0280008206.01.T01:exon:18785;Parent=MsG0280008206.01.T01 |
| Chr2 | exon | 27185150 | 27185290 | 27185150 | ID=MsG0280008206.01.T01:exon:18784;Parent=MsG0280008206.01.T01 |
| Chr2 | exon | 27184905 | 27185067 | 27184905 | ID=MsG0280008206.01.T01:exon:18783;Parent=MsG0280008206.01.T01 |
| Chr2 | CDS | 27185389 | 27185534 | 27185389 | ID=MsG0280008206.01.T01:cds;Parent=MsG0280008206.01.T01 |
| Chr2 | CDS | 27185150 | 27185290 | 27185150 | ID=MsG0280008206.01.T01:cds;Parent=MsG0280008206.01.T01 |
| Chr2 | CDS | 27184905 | 27185067 | 27184905 | ID=MsG0280008206.01.T01:cds;Parent=MsG0280008206.01.T01 |
| Gene Sequence |
| Protein sequence |
