AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017286.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017286.01.T01 MTR_3g104640 100.000 110 0 0 1 110 1 110 1.70e-74 216
MsG0380017286.01.T01 MTR_4g125990 55.906 127 35 5 1 110 1 123 8.82e-35 116
MsG0380017286.01.T01 MTR_1g015810 67.241 116 30 5 1 108 1 116 6.66e-32 108
MsG0380017286.01.T01 MTR_2g099630 49.541 109 44 4 6 109 4 106 4.86e-26 93.6
MsG0380017286.01.T01 MTR_8g018320 50.495 101 49 1 6 106 4 103 7.14e-22 83.2
MsG0380017286.01.T01 MTR_8g018360 50.000 102 50 1 6 107 4 104 7.42e-21 82.8
MsG0380017286.01.T01 MTR_7g109290 46.667 60 32 0 51 110 32 91 5.46e-14 62.4
MsG0380017286.01.T01 MTR_1g098310 57.407 54 20 1 56 109 35 85 2.62e-13 60.8
MsG0380017286.01.T01 MTR_1g087730 42.373 59 34 0 52 110 48 106 9.93e-13 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017286.01.T01 AT5G11970 59.091 110 39 3 1 110 1 104 1.86e-35 117
MsG0380017286.01.T01 AT2G19460 45.455 121 46 5 1 107 1 115 4.28e-21 81.6
MsG0380017286.01.T01 AT2G19460 45.455 121 46 5 1 107 53 167 2.02e-20 81.3
MsG0380017286.01.T01 AT2G47480 51.613 62 27 1 50 108 47 108 5.95e-15 65.5
MsG0380017286.01.T01 AT3G13910 58.621 58 23 1 45 101 40 97 2.56e-14 63.9
MsG0380017286.01.T01 AT3G13910 58.621 58 23 1 45 101 40 97 5.56e-14 63.2
MsG0380017286.01.T01 AT1G72720 62.222 45 17 0 65 109 81 125 6.49e-14 63.5
MsG0380017286.01.T01 AT3G62640 51.111 45 22 0 65 109 66 110 6.38e-13 60.5
MsG0380017286.01.T01 AT3G05725 35.115 131 55 7 1 109 1 123 1.66e-12 59.7
MsG0380017286.01.T01 AT4G09890 53.846 39 18 0 66 104 39 77 5.75e-11 54.7

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTACTCACAGGTGGTTTA+TGG 0.228498 3:-94492364 MsG0380017286.01.T01:CDS
CTCTTCTTCCTCTGAAGTTC+AGG 0.289847 3:+94492466 None:intergenic
AGCTTTCTATCTGCATCATT+TGG 0.373432 3:+94492629 None:intergenic
TATAAAATGTATTCTGTTGA+AGG 0.385236 3:-94492433 MsG0380017286.01.T01:CDS
CTAAGATCATAAGGCTTAGA+TGG 0.404348 3:+94492598 None:intergenic
ATGCAGATAGAAAGCTACTA+TGG 0.407129 3:-94492622 MsG0380017286.01.T01:CDS
TATCTGCATCATTTGGCCAT+TGG 0.440208 3:+94492636 None:intergenic
CTATAGCTTCTAAGATCATA+AGG 0.444134 3:+94492589 None:intergenic
CTGAACTTCAGAGGAAGAAG+AGG 0.478478 3:-94492465 MsG0380017286.01.T01:CDS
TTCTTCAACTTCAAATCCTT+AGG 0.481282 3:+94492544 None:intergenic
AAGGATTTGAAGTTGAAGAA+AGG 0.511800 3:-94492541 MsG0380017286.01.T01:CDS
CTTAGGAGTTTGTTGTACAT+AGG 0.522202 3:+94492561 None:intergenic
CTATGTACAACAAACTCCTA+AGG 0.555597 3:-94492560 MsG0380017286.01.T01:CDS
TCTGTTGAAGGCAAAGTCAA+AGG 0.571369 3:-94492421 MsG0380017286.01.T01:CDS
CTTTCAGAAAGAGTTTCAGA+TGG 0.576426 3:-94492396 MsG0380017286.01.T01:CDS
ACTCACAGGTGGTTTATGGT+TGG 0.580184 3:-94492360 MsG0380017286.01.T01:CDS
TGTCTGATCCTGAACTTCAG+AGG 0.591643 3:-94492474 MsG0380017286.01.T01:CDS
AGATCAAAATCTTATGCCAA+TGG 0.604385 3:-94492652 MsG0380017286.01.T01:CDS
GCTTAAGAACAAGTACTCAC+AGG 0.640169 3:-94492374 MsG0380017286.01.T01:CDS
TGAACTTCAGAGGAAGAAGA+GGG 0.657165 3:-94492464 MsG0380017286.01.T01:CDS
TAAGAACAAGTACTCACAGG+TGG 0.685797 3:-94492371 MsG0380017286.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAATGTATTCTGTTGA+AGG - Chr3:94492591-94492610 MsG0380017286.01.T01:CDS 20.0%
AGATCAAAATCTTATGCCAA+TGG - Chr3:94492372-94492391 MsG0380017286.01.T01:CDS 30.0%
TTCTTCAACTTCAAATCCTT+AGG + Chr3:94492483-94492502 None:intergenic 30.0%
! AAAGGTAAAAGCTTTTCATC+TGG - Chr3:94492501-94492520 MsG0380017286.01.T01:CDS 30.0%
! AAGGATTTGAAGTTGAAGAA+AGG - Chr3:94492483-94492502 MsG0380017286.01.T01:CDS 30.0%
!! CTATAGCTTCTAAGATCATA+AGG + Chr3:94492438-94492457 None:intergenic 30.0%
AGCTTTCTATCTGCATCATT+TGG + Chr3:94492398-94492417 None:intergenic 35.0%
ATGCAGATAGAAAGCTACTA+TGG - Chr3:94492402-94492421 MsG0380017286.01.T01:CDS 35.0%
CTAAGATCATAAGGCTTAGA+TGG + Chr3:94492429-94492448 None:intergenic 35.0%
CTATGTACAACAAACTCCTA+AGG - Chr3:94492464-94492483 MsG0380017286.01.T01:CDS 35.0%
CTTTCAGAAAGAGTTTCAGA+TGG - Chr3:94492628-94492647 MsG0380017286.01.T01:CDS 35.0%
!! CTTAGGAGTTTGTTGTACAT+AGG + Chr3:94492466-94492485 None:intergenic 35.0%
AAGTACTCACAGGTGGTTTA+TGG - Chr3:94492660-94492679 MsG0380017286.01.T01:CDS 40.0%
GCTTAAGAACAAGTACTCAC+AGG - Chr3:94492650-94492669 MsG0380017286.01.T01:CDS 40.0%
TAAGAACAAGTACTCACAGG+TGG - Chr3:94492653-94492672 MsG0380017286.01.T01:CDS 40.0%
TATCTGCATCATTTGGCCAT+TGG + Chr3:94492391-94492410 None:intergenic 40.0%
TCTGTTGAAGGCAAAGTCAA+AGG - Chr3:94492603-94492622 MsG0380017286.01.T01:CDS 40.0%
TGAACTTCAGAGGAAGAAGA+GGG - Chr3:94492560-94492579 MsG0380017286.01.T01:CDS 40.0%
ACTCACAGGTGGTTTATGGT+TGG - Chr3:94492664-94492683 MsG0380017286.01.T01:CDS 45.0%
CTGAACTTCAGAGGAAGAAG+AGG - Chr3:94492559-94492578 MsG0380017286.01.T01:CDS 45.0%
TGTCTGATCCTGAACTTCAG+AGG - Chr3:94492550-94492569 MsG0380017286.01.T01:CDS 45.0%
!! CTCTTCTTCCTCTGAAGTTC+AGG + Chr3:94492561-94492580 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr3 gene 94492357 94492689 94492357 ID=MsG0380017286.01;Name=MsG0380017286.01
Chr3 mRNA 94492357 94492689 94492357 ID=MsG0380017286.01.T01;Parent=MsG0380017286.01;Name=MsG0380017286.01.T01;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|110
Chr3 exon 94492357 94492689 94492357 ID=MsG0380017286.01.T01:exon:1511;Parent=MsG0380017286.01.T01
Chr3 CDS 94492357 94492689 94492357 ID=MsG0380017286.01.T01:cds;Parent=MsG0380017286.01.T01
Gene Sequence

>MsG0380017286.01.T01

ATGGAAGATTTTCAGAGATCAAAATCTTATGCCAATGGCCAAATGATGCAGATAGAAAGCTACTATGGACCATCTAAGCCTTATGATCTTAGAAGCTATAGTTCTTCCTATGTACAACAAACTCCTAAGGATTTGAAGTTGAAGAAAGGTAAAAGCTTTTCATCTGGTTCTTCTTTTTCAAAGTCTTTGAGTTTGTCTGATCCTGAACTTCAGAGGAAGAAGAGGGTTGCTAGCTATAAAATGTATTCTGTTGAAGGCAAAGTCAAAGGATCTTTCAGAAAGAGTTTCAGATGGCTTAAGAACAAGTACTCACAGGTGGTTTATGGTTGGTAG

Protein sequence

>MsG0380017286.01.T01

MEDFQRSKSYANGQMMQIESYYGPSKPYDLRSYSSSYVQQTPKDLKLKKGKSFSSGSSFSKSLSLSDPELQRKKRVASYKMYSVEGKVKGSFRKSFRWLKNKYSQVVYGW*