Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008466.01.T01 | XP_013463600.1 | 87.097 | 186 | 20 | 2 | 1 | 186 | 1 | 182 | 2.90E-106 | 322 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008466.01.T01 | A0A072VHK0 | 87.097 | 186 | 20 | 2 | 1 | 186 | 1 | 182 | 1.39e-106 | 322 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000502.01 | MsG0280008466.01 | 0.802896 | 4.513859e-49 | 2.237080e-46 |
| MsG0180003649.01 | MsG0280008466.01 | 0.802090 | 6.616511e-49 | 3.212478e-46 |
| MsG0180003949.01 | MsG0280008466.01 | 0.853670 | 2.050186e-61 | 4.463900e-58 |
| MsG0180004187.01 | MsG0280008466.01 | 0.810893 | 9.190305e-51 | 5.607779e-48 |
| MsG0180004302.01 | MsG0280008466.01 | 0.827452 | 1.552742e-54 | 1.499700e-51 |
| MsG0180004937.01 | MsG0280008466.01 | 0.863362 | 2.628696e-64 | 7.948317e-61 |
| MsG0180004987.01 | MsG0280008466.01 | 0.814096 | 1.833098e-51 | 1.218624e-48 |
| MsG0280006566.01 | MsG0280008466.01 | 0.800276 | 1.555298e-48 | 7.212149e-46 |
| MsG0280007142.01 | MsG0280008466.01 | 0.805024 | 1.629989e-49 | 8.532058e-47 |
| MsG0280008466.01 | MsG0280010618.01 | 0.820582 | 6.356228e-53 | 5.049121e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008466.01.T01 | MTR_2g043710 | 87.097 | 186 | 20 | 2 | 1 | 186 | 1 | 182 | 3.52e-110 | 322 |
| MsG0280008466.01.T01 | MTR_4g124850 | 40.306 | 196 | 78 | 10 | 1 | 186 | 1 | 167 | 3.73e-27 | 107 |
| MsG0280008466.01.T01 | MTR_8g057880 | 48.760 | 121 | 50 | 4 | 193 | 306 | 19 | 134 | 1.65e-26 | 107 |
| MsG0280008466.01.T01 | MTR_4g078000 | 60.714 | 84 | 31 | 1 | 226 | 307 | 26 | 109 | 2.72e-26 | 106 |
| MsG0280008466.01.T01 | MTR_7g113060 | 62.651 | 83 | 29 | 1 | 226 | 306 | 55 | 137 | 1.46e-25 | 102 |
| MsG0280008466.01.T01 | MTR_8g015280 | 63.095 | 84 | 29 | 1 | 226 | 307 | 36 | 119 | 2.61e-25 | 100 |
| MsG0280008466.01.T01 | MTR_7g117680 | 55.952 | 84 | 35 | 1 | 226 | 307 | 28 | 111 | 2.77e-24 | 97.1 |
| MsG0280008466.01.T01 | MTR_7g027013 | 60.465 | 86 | 32 | 1 | 224 | 307 | 578 | 663 | 3.53e-24 | 105 |
| MsG0280008466.01.T01 | MTR_3g030470 | 65.476 | 84 | 27 | 1 | 226 | 307 | 84 | 167 | 3.64e-24 | 99.8 |
| MsG0280008466.01.T01 | MTR_4g062370 | 63.953 | 86 | 29 | 1 | 224 | 307 | 573 | 658 | 7.42e-24 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008466.01.T01 | AT5G20790 | 38.172 | 186 | 100 | 7 | 5 | 186 | 4 | 178 | 7.04e-23 | 95.5 |
| MsG0280008466.01.T01 | AT3G43110 | 35.106 | 188 | 93 | 8 | 5 | 186 | 4 | 168 | 4.36e-19 | 85.1 |
| MsG0280008466.01.T01 | AT5G20790 | 36.364 | 132 | 74 | 4 | 5 | 135 | 4 | 126 | 5.40e-14 | 69.3 |
Find 91 sgRNAs with CRISPR-Local
Find 710 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAATTCTTACGTCAAA+TGG | 0.376512 | 2:+32413692 | None:intergenic |
| AACAAATTACAAGTATGCAA+TGG | 0.593688 | 2:-32413821 | MsG0280008466.01.T01:CDS |
| AACAGACTTCTGGAAACATC+TGG | 0.466195 | 2:-32405586 | MsG0280008466.01.T01:intron |
| AACCGAAAGTTGAAGGTGAG+TGG | 0.681098 | 2:-32416766 | MsG0280008466.01.T01:CDS |
| AACGGAGCTTAATGGAAACG+TGG | 0.662549 | 2:-32416669 | MsG0280008466.01.T01:intron |
| AAGAGTGGTAGTAATTGTTA+CGG | 0.506110 | 2:-32416815 | MsG0280008466.01.T01:CDS |
| AAGGTGAGTGGTGGAAATGT+TGG | 0.461562 | 2:-32416754 | MsG0280008466.01.T01:CDS |
| AAGGTGTTAAGGTTGAGAAA+TGG | 0.424600 | 2:-32416728 | MsG0280008466.01.T01:CDS |
| AAGTTGGAGTGTTAGTTATG+CGG | 0.601843 | 2:-32413853 | MsG0280008466.01.T01:CDS |
| AATCTTCCAGAAGCTGAGAG+CGG | 0.686867 | 2:+32417096 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAATTAACATTCCTGGG+AGG | + | Chr2:32413193-32413212 | None:intergenic | 30.0% | |
| AACATAGCTAACTACTAACA+GGG | + | Chr2:32413002-32413021 | None:intergenic | 30.0% | |
| AAGAGTGGTAGTAATTGTTA+CGG | - | Chr2:32405715-32405734 | MsG0280008466.01.T01:intron | 30.0% | |
| AAGTTGAAGCAAACAATTTG+AGG | + | Chr2:32410790-32410809 | None:intergenic | 30.0% | |
| AATGTGTAGTCCAAATCAAT+CGG | - | Chr2:32410660-32410679 | MsG0280008466.01.T01:intron | 30.0% | |
| AATGTGTGTTCTGATCTAAT+GGG | + | Chr2:32414768-32414787 | None:intergenic | 30.0% | |
| AATTGTTTGCTTCAACTTCT+TGG | - | Chr2:32410792-32410811 | MsG0280008466.01.T01:intron | 30.0% | |
| ACAGTAAATATACACAGTCA+GGG | + | Chr2:32413828-32413847 | None:intergenic | 30.0% | |
| ACAGTAAGTATACACAGTTA+GGG | + | Chr2:32413900-32413919 | None:intergenic | 30.0% | |
| ACCAAAGTTTAAGTAGTTGA+AGG | + | Chr2:32405368-32405387 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 32405328 | 32417224 | 32405328 | ID=MsG0280008466.01;Name=MsG0280008466.01 |
| Chr2 | mRNA | 32405328 | 32417224 | 32405328 | ID=MsG0280008466.01.T01;Parent=MsG0280008466.01;Name=MsG0280008466.01.T01;_AED=0.48;_eAED=0.60;_QI=0|0|0|1|1|1|4|0|397 |
| Chr2 | exon | 32416670 | 32417224 | 32416670 | ID=MsG0280008466.01.T01:exon:22620;Parent=MsG0280008466.01.T01 |
| Chr2 | exon | 32415227 | 32415344 | 32415227 | ID=MsG0280008466.01.T01:exon:22619;Parent=MsG0280008466.01.T01 |
| Chr2 | exon | 32413706 | 32413950 | 32413706 | ID=MsG0280008466.01.T01:exon:22618;Parent=MsG0280008466.01.T01 |
| Chr2 | exon | 32405328 | 32405603 | 32405328 | ID=MsG0280008466.01.T01:exon:22617;Parent=MsG0280008466.01.T01 |
| Chr2 | CDS | 32416670 | 32417224 | 32416670 | ID=MsG0280008466.01.T01:cds;Parent=MsG0280008466.01.T01 |
| Chr2 | CDS | 32415227 | 32415344 | 32415227 | ID=MsG0280008466.01.T01:cds;Parent=MsG0280008466.01.T01 |
| Chr2 | CDS | 32413706 | 32413950 | 32413706 | ID=MsG0280008466.01.T01:cds;Parent=MsG0280008466.01.T01 |
| Chr2 | CDS | 32405328 | 32405603 | 32405328 | ID=MsG0280008466.01.T01:cds;Parent=MsG0280008466.01.T01 |
| Gene Sequence |
>MsG0280008466.01.T01 ATGAACCTCTTGTTAGATTCCAACGTTGAGGCTTTAGCCTTCAACTACTTAAACTTTGGTTTAATCACAATTCTCAACAACTTATGGACTTGGCTTGCTATCACTGCCGCTCTCAGCTTCTGGAAGATTCGCTCATCCGGTTGTCCCAAATCACTAGACCCAGTTTCAGTAAAACCGGATCAAGGTGTCTCAGTTTCCGCTGTTAAATCTTCGGTGGAAATAAAACCATACCAGAGTGTCTTAATTTCCACCGTTACAGATGAAGATGTAACGTCGGAAACTGAGACGCCTCGAAAAACGTCATCAAATGTTTTTGATGATGTTGACGGTGTGAGGAAGGGGAAGTTTACGTTGTATTACGAAGATGACATGCAATGTGGATTCGACAAGAGTGGTAGTAATTGTTACGGGCAATTGCCGGTTGTTGAAGGGTGGGAACCGAAAGTTGAAGGTGAGTGGTGGAAATGTTGGGAGAAGGTGTTAAGGTTGAGAAATGGAGAGAATGAGAATGGGTGGTACACGTGTCAGGATTTAACGGAGCTTAATGGAAACGTGGTTGTTGAGCCACAAGTTAGCAAACTATGCATGGAGGTTGAAGAATTCATTTTTATGGAAAAGTGGTTGGAGCTGGTATCGTTGATGATTACTTATGCTGAATTGAAATTTCTCAAAGGGAATGATGTTCACACTCCTAAATTTCTCAAAACAATTACTATTTTGGGTCTTTCAAATCTCAAACTGAAATTAAAAGTTGGAGTGTTAGTTATGCGGAATATAGATAACAAATTACAAGTATGCAATGGAACAAGACTGACAATAACAAGGATGAGAAAATATGTGCTTGAAAGAAAGCTGATATCGGAATATAATATCGACGACAAAGTTTTTATACCTAGGTTATCTCTGACACCATTTGACACTTCTGGAAACATCTGGAAGTATCCTAAACAACCCTCATTCTTACTCCTAAGACCAACTCATGACTACACGTGTGAAAGGATCGCAAGGGAAACAAATTGGGCACTAACAGTGTTACTCGCAATGGCGAGTACAATTGCTCGCACCGGCGAGCAAAATGTGCTCTCTGGAACTCCTAAAGGACTTGCTCGCAACGACGAGCTACTTTGCTCGCAATGGCGAGTACTGGGATTATTCGCAATGGCGAGGGTTCTAGCTCGAAATGGCGAGCGATGA |
| Protein sequence |
>MsG0280008466.01.T01 MNLLLDSNVEALAFNYLNFGLITILNNLWTWLAITAALSFWKIRSSGCPKSLDPVSVKPDQGVSVSAVKSSVEIKPYQSVLISTVTDEDVTSETETPRKTSSNVFDDVDGVRKGKFTLYYEDDMQCGFDKSGSNCYGQLPVVEGWEPKVEGEWWKCWEKVLRLRNGENENGWYTCQDLTELNGNVVVEPQVSKLCMEVEEFIFMEKWLELVSLMITYAELKFLKGNDVHTPKFLKTITILGLSNLKLKLKVGVLVMRNIDNKLQVCNGTRLTITRMRKYVLERKLISEYNIDDKVFIPRLSLTPFDTSGNIWKYPKQPSFLLLRPTHDYTCERIARETNWALTVLLAMASTIARTGEQNVLSGTPKGLARNDELLCSQWRVLGLFAMARVLARNGER* |
