AlfalfaGEDB | MsG0280008689.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008689.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008689.01.T01	XP_003595473.1	91.028	457	40	1	1	457	1	456	0	850

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008689.01.T01	Q9C7X8	31.961	413	258	10	16	414	22	425	1.51E-57	200

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008689.01.T01	G7ISV8	91.028	457	40	1	1	457	1	456	0.0	850

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0280008687.01	MsG0280008689.01	PPI
MsG0280008689.01	MsG0580028297.01	PPI
MsG0280008689.01	MsG0580024319.01	PPI
MsG0280008689.01	MsG0780038508.01	PPI
MsG0280008689.01	MsG0280007849.01	PPI
MsG0280008689.01	MsG0480022017.01	PPI
MsG0280008689.01	MsG0780037872.01	PPI
MsG0280008689.01	MsG0780038510.01	PPI
MsG0280008689.01	MsG0780037227.01	PPI
MsG0280008689.01	MsG0780038148.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008689.01.T01	MTR_2g048140	91.028	457	40	1	1	457	1	456	0.0	850
MsG0280008689.01.T01	MTR_1g090907	80.525	457	87	2	1	457	1	455	0.0	737
MsG0280008689.01.T01	MTR_1g090903	59.307	462	132	6	1	457	1	411	3.73e-175	497
MsG0280008689.01.T01	MTR_3g448340	36.933	463	271	11	5	454	4	458	1.63e-82	262
MsG0280008689.01.T01	MTR_5g080150	34.975	406	224	7	5	404	3	374	4.27e-67	220
MsG0280008689.01.T01	MTR_3g049530	30.146	481	237	15	5	455	4	415	1.02e-45	164
MsG0280008689.01.T01	MTR_1g071060	73.333	90	24	0	40	129	7	96	2.61e-38	134
MsG0280008689.01.T01	MTR_7g029260	34.921	126	81	1	304	428	34	159	9.20e-17	79.7
MsG0280008689.01.T01	MTR_2g047858	23.782	349	213	14	20	353	128	438	7.02e-14	73.9
MsG0280008689.01.T01	MTR_8g468470	21.814	408	275	16	30	414	36	422	5.22e-13	71.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008689.01.T01	AT1G56400	31.961	413	258	10	16	414	22	425	1.54e-58	200
MsG0280008689.01.T01	AT5G54820	29.979	467	295	13	9	454	9	464	7.84e-57	195
MsG0280008689.01.T01	AT3G29830	32.196	469	273	18	10	454	11	458	1.98e-55	191
MsG0280008689.01.T01	AT3G29830	31.557	469	277	17	10	454	11	459	8.24e-54	187
MsG0280008689.01.T01	AT3G62230	28.571	427	287	13	6	422	7	425	1.24e-47	170
MsG0280008689.01.T01	AT1G56400	32.092	349	214	9	80	414	45	384	2.40e-46	166
MsG0280008689.01.T01	AT1G61330	27.346	373	232	13	6	361	9	359	5.88e-20	92.4
MsG0280008689.01.T01	AT2G17305	30.120	166	108	4	296	456	106	268	4.25e-19	87.4
MsG0280008689.01.T01	AT5G56410	31.174	247	146	9	16	248	12	248	2.13e-15	79.0
MsG0280008689.01.T01	AT3G28410	23.951	405	248	18	4	392	25	385	6.36e-12	67.8

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Find 70 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCACTTTGCATCTTCAC+CGG	0.537449	2:-36390690	None:intergenic
AAATCGTTATCTTCCCATTT+TGG	0.155222	2:-36389919	None:intergenic
AAATGTTAGGGCTATAGAAG+AGG	0.577818	2:+36390311	MsG0280008689.01.T01:CDS
AACAGATTAATATTAAGTTG+TGG	0.543730	2:+36391106	MsG0280008689.01.T01:CDS
AACCATGGCAAGAGATAACA+TGG	0.562222	2:+36389534	None:intergenic
AACCTGTTAATGCCGCGGCT+AGG	0.547285	2:-36389790	None:intergenic
AACGGTTAGAATCTTGGCGG+CGG	0.607837	2:-36390409	None:intergenic
AAGGATAGATGTTCTTGTTG+TGG	0.519897	2:-36389623	None:intergenic
AAGTCCACAAGTTATATTTG+AGG	0.508872	2:+36390821	MsG0280008689.01.T01:CDS
AATTTGGCGAACTTCGATTT+GGG	0.411419	2:+36390168	MsG0280008689.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTCCACAAGTTATATTTG+AGG	+	Chr2:36390821-36390840	MsG0280008689.01.T01:CDS	30.0%
ATGCATTCATATGAACTTTG+TGG	+	Chr2:36390744-36390763	MsG0280008689.01.T01:CDS	30.0%
ATTGCTTGAAAAACTCACAT+TGG	+	Chr2:36390794-36390813	MsG0280008689.01.T01:CDS	30.0%
CCGAAAAATGGTTAAAGATA+TGG	+	Chr2:36389647-36389666	MsG0280008689.01.T01:CDS	30.0%
CTTCAATGAAAACTTCTTTG+TGG	+	Chr2:36389690-36389709	MsG0280008689.01.T01:CDS	30.0%
CTTGAAAAATACATTGAAGG+TGG	+	Chr2:36391008-36391027	MsG0280008689.01.T01:CDS	30.0%
GAGTTTGCCAATATTTCTTA+AGG	-	Chr2:36390969-36390988	None:intergenic	30.0%
TGATTTCTCTGATCCAAAAT+GGG	+	Chr2:36389906-36389925	MsG0280008689.01.T01:CDS	30.0%
TTCTTAAGGTCTACATCATA+GGG	-	Chr2:36390955-36390974	None:intergenic	30.0%
TTGAATCGCTAAAACTGTAT+TGG	+	Chr2:36390001-36390020	MsG0280008689.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	36389538	36391227	36389538	ID=MsG0280008689.01;Name=MsG0280008689.01
Chr2	mRNA	36389538	36391227	36389538	ID=MsG0280008689.01.T01;Parent=MsG0280008689.01;Name=MsG0280008689.01.T01;_AED=0.18;_eAED=0.18;_QI=0\|0\|0\|1\|1\|1\|3\|0\|457
Chr2	exon	36389538	36390449	36389538	ID=MsG0280008689.01.T01:exon:15473;Parent=MsG0280008689.01.T01
Chr2	exon	36390669	36390842	36390669	ID=MsG0280008689.01.T01:exon:15474;Parent=MsG0280008689.01.T01
Chr2	exon	36390940	36391227	36390940	ID=MsG0280008689.01.T01:exon:15475;Parent=MsG0280008689.01.T01
Chr2	CDS	36389538	36390449	36389538	ID=MsG0280008689.01.T01:cds;Parent=MsG0280008689.01.T01
Chr2	CDS	36390669	36390842	36390669	ID=MsG0280008689.01.T01:cds;Parent=MsG0280008689.01.T01
Chr2	CDS	36390940	36391227	36390940	ID=MsG0280008689.01.T01:cds;Parent=MsG0280008689.01.T01

Gene Sequence

>MsG0280008689.01.T01

ATGGCAAGAGATAACATGGAATTGTTTTCTTCGATACTAAAACCACTTCTTCTTGCAATCATTTCTTTCCTACCCTCCGAAGAAGCCACAACAAGAACATCTATCCTTACCGAAAAATGGTTAAAGATATGGCAGTCCGAAGAAAACATCGACTTCAATGAAAACTTCTTTGTGGATTCAACTTCTGATGAAAACAAACAAGAACAAAGAAAAGTTTTCATCAACTTTATTACAACTTGGATCACACACTTCCCTAGCCGCGGCATTAACAGGTTTTCTCTCACGGTTTTGAACCCTCAAACTTGTGGGGAAACAATTGAGCAGTGTGTTGCTTTCGCGATACAACGGGGCGTGAAAGAGTTAATTCTTGATTTCTCTGATCCAAAATGGGAAGATAACGATTTTGATGGTAAACATGCGTTGTTTCAATTGCCAACGCATGTTTATCAACTCGGATTGTCGCTTGAATCGCTAAAACTGTATTGGTGTGGTTTCAATACGCCAGATTTTCTCAACTTTGGTGCACTCAAAGACTTATCTTTGGGTCGGATTGAAGTTAAAATCGAAACTCTAAAAAGATTGTTATCAACCTGTCGAACAATTGAAAGTTTGAGTCTTGAGAAGTGTTGGAATTTGGCGAACTTCGATTTGGGAGATGAACCACTTGGATTAACAAGATTGGTGGTAAACAAGTGTGATTCTGAGTATTTCATATTTAATGCACCAAATCTTAAATACTTCAAATATTCTGGAGTTGTGTTTACTTCAGATATAAATGTTAGGGCTATAGAAGAGGTAGACATTGATTTTTCTCTAGAATCAGAATTCGATGAACGTGGTAATGAACTCTGCCAAATCCTTCATGATTTCTCCGCCGCCAAGATTCTAACCGTTTGCAGTTATCTACTTCAGGTCGTTCCATCTCGAGATGAACCGGTGAAGATGCAAAGTGGTTTGAATGTAAAACATTTGACTCTAAAGATTCAAATGCATTCATATGAACTTTGTGGATTTGAATTCATGCTCAACAGTTGCCCATTGCTTGAAAAACTCACATTGGACATAAGTCCACAAGTTATATTTGAGGACTATACACTTCCCTATGATGTAGACCTTAAGAAATATTGGCAAACTCATCGAGTGTTTCCTCAATGCTTGAAAAATACATTGAAGGTGGTAGAAGTAATTGGATTGAGAGCCACAAACGAAGAACTTATGACTTGTTGCTTTCTTATGCAAGGAAAAGTTTTGAAACAGATTAATATTAAGTTGTGGAATGAAGATGATGATAAAGAAGAATTTCGACGTGGATGTGCACAACTTTTAGTCAAAGCTCAAAAGGGTTCAAAGAATTTGCAAATATCAATTGATTGA

Protein sequence

>MsG0280008689.01.T01

MARDNMELFSSILKPLLLAIISFLPSEEATTRTSILTEKWLKIWQSEENIDFNENFFVDSTSDENKQEQRKVFINFITTWITHFPSRGINRFSLTVLNPQTCGETIEQCVAFAIQRGVKELILDFSDPKWEDNDFDGKHALFQLPTHVYQLGLSLESLKLYWCGFNTPDFLNFGALKDLSLGRIEVKIETLKRLLSTCRTIESLSLEKCWNLANFDLGDEPLGLTRLVVNKCDSEYFIFNAPNLKYFKYSGVVFTSDINVRAIEEVDIDFSLESEFDERGNELCQILHDFSAAKILTVCSYLLQVVPSRDEPVKMQSGLNVKHLTLKIQMHSYELCGFEFMLNSCPLLEKLTLDISPQVIFEDYTLPYDVDLKKYWQTHRVFPQCLKNTLKVVEVIGLRATNEELMTCCFLMQGKVLKQINIKLWNEDDDKEEFRRGCAQLLVKAQKGSKNLQISID*