AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038508.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038508.01.T01 MTR_7g056433 58.824 323 126 4 67 385 65 384 4.45e-117 346
MsG0780038508.01.T01 MTR_7g115270 54.328 335 133 6 68 385 66 397 3.13e-107 321
MsG0780038508.01.T01 MTR_5g013500 49.240 329 131 8 72 385 53 360 1.49e-90 277
MsG0780038508.01.T01 MTR_7g098510 42.202 327 178 7 64 386 68 387 3.84e-71 228
MsG0780038508.01.T01 MTR_7g056420 47.493 339 129 10 67 392 62 364 1.15e-70 240
MsG0780038508.01.T01 MTR_7g056430 47.493 339 129 10 67 392 62 364 1.37e-70 240
MsG0780038508.01.T01 MTR_1g041245 44.377 329 160 12 68 385 65 381 3.69e-70 226
MsG0780038508.01.T01 MTR_7g098570 42.813 327 176 7 64 386 70 389 5.21e-66 218
MsG0780038508.01.T01 MTR_7g032370 41.975 324 162 11 68 385 44 347 2.30e-63 207
MsG0780038508.01.T01 MTR_7g098480 41.411 326 174 9 64 383 68 382 4.11e-61 204
MsG0780038508.01.T01 MTR_7g032380 42.202 327 141 14 68 385 64 351 1.64e-58 194
MsG0780038508.01.T01 MTR_7g033915 40.615 325 157 10 68 385 65 360 7.61e-58 193
MsG0780038508.01.T01 MTR_7g034115 41.720 314 166 10 68 375 65 367 9.27e-57 191
MsG0780038508.01.T01 MTR_3g036250 41.772 316 149 10 80 385 71 361 1.05e-56 190
MsG0780038508.01.T01 MTR_7g088260 38.485 330 188 9 61 385 138 457 1.85e-56 192
MsG0780038508.01.T01 MTR_7g034120 41.358 324 152 14 78 385 2 303 1.14e-55 186
MsG0780038508.01.T01 MTR_7g033990 42.812 320 154 11 69 377 68 369 8.23e-54 184
MsG0780038508.01.T01 MTR_3g108560 38.438 333 182 10 61 385 67 384 3.08e-50 174
MsG0780038508.01.T01 MTR_3g040700 37.107 318 166 8 64 373 93 384 1.85e-49 173
MsG0780038508.01.T01 MTR_3g108150 36.937 333 192 11 61 385 60 382 2.96e-47 166
MsG0780038508.01.T01 MTR_3g082890 36.556 331 180 7 71 378 151 474 7.21e-47 167
MsG0780038508.01.T01 MTR_0492s0020 35.625 320 181 9 62 375 134 434 1.07e-46 166
MsG0780038508.01.T01 MTR_0543s0010 35.625 320 181 9 62 375 134 434 1.93e-46 165
MsG0780038508.01.T01 MTR_5g012840 34.356 326 151 9 68 385 43 313 2.20e-46 162
MsG0780038508.01.T01 MTR_3g023630 36.615 325 186 11 64 383 58 367 2.13e-45 160
MsG0780038508.01.T01 MTR_3g082890 32.880 368 180 7 71 378 71 431 7.13e-42 152
MsG0780038508.01.T01 MTR_8g085430 37.864 309 112 10 75 377 80 314 2.24e-41 149
MsG0780038508.01.T01 MTR_3g467400 36.119 335 168 12 68 385 57 362 5.02e-41 149
MsG0780038508.01.T01 MTR_5g007410 35.540 287 166 8 86 362 102 379 6.35e-41 150
MsG0780038508.01.T01 MTR_3g109982 38.000 250 140 6 135 377 220 461 3.46e-40 152
MsG0780038508.01.T01 MTR_3g109982 36.957 138 72 5 68 201 67 193 6.56e-13 70.9
MsG0780038508.01.T01 MTR_6g045333 34.194 310 189 9 86 386 139 442 1.27e-39 147
MsG0780038508.01.T01 MTR_5g008920 32.335 334 200 12 63 385 58 376 1.36e-38 142
MsG0780038508.01.T01 MTR_3g087260 37.722 281 151 10 116 385 185 452 2.79e-38 143
MsG0780038508.01.T01 MTR_3g087260 37.879 132 70 5 100 224 82 208 1.20e-12 69.3
MsG0780038508.01.T01 MTR_3g071850 34.146 287 173 9 86 363 102 381 3.08e-37 144
MsG0780038508.01.T01 MTR_7g056277 34.954 329 190 10 69 389 315 627 4.21e-37 142
MsG0780038508.01.T01 MTR_3g107100 31.871 342 198 11 67 385 64 393 1.30e-36 138
MsG0780038508.01.T01 MTR_2g007450 31.847 314 163 11 86 360 93 394 5.74e-36 136
MsG0780038508.01.T01 MTR_5g045170 32.344 337 213 8 64 391 62 392 7.53e-36 135
MsG0780038508.01.T01 MTR_7g056287 34.036 332 189 11 69 389 59 371 2.05e-35 134
MsG0780038508.01.T01 MTR_5g008950 32.808 317 185 12 86 390 65 365 3.55e-35 133
MsG0780038508.01.T01 MTR_7g056020 33.233 331 173 8 80 388 75 379 6.92e-34 133
MsG0780038508.01.T01 MTR_7g056447 56.589 129 51 2 67 195 62 185 1.15e-33 127
MsG0780038508.01.T01 MTR_3g105060 32.508 323 195 10 67 386 67 369 2.64e-33 128
MsG0780038508.01.T01 MTR_7g026500 31.447 318 180 12 67 362 67 368 6.96e-33 127
MsG0780038508.01.T01 MTR_5g073420 31.475 305 183 10 61 358 59 344 3.41e-32 125
MsG0780038508.01.T01 MTR_7g114710 33.229 319 171 12 75 385 75 359 5.45e-32 124
MsG0780038508.01.T01 MTR_5g039470 30.293 307 174 11 69 355 42 328 1.17e-31 122
MsG0780038508.01.T01 MTR_5g058000 30.702 342 211 11 51 385 48 370 2.85e-31 122
MsG0780038508.01.T01 MTR_1089s0010 31.307 329 188 13 63 377 54 358 3.44e-31 122
MsG0780038508.01.T01 MTR_7g007080 30.539 334 186 13 80 392 73 381 4.00e-31 122
MsG0780038508.01.T01 MTR_6g040270 31.511 311 184 11 86 385 84 376 4.65e-31 122
MsG0780038508.01.T01 MTR_5g063950 31.392 309 196 7 84 386 60 358 6.34e-31 121
MsG0780038508.01.T01 MTR_5g031970 34.058 276 155 7 84 358 74 323 8.31e-31 121
MsG0780038508.01.T01 MTR_1g108820 30.357 336 202 12 67 385 61 381 1.46e-30 120
MsG0780038508.01.T01 MTR_0009s0080 32.095 296 172 7 86 366 94 375 2.46e-30 121
MsG0780038508.01.T01 MTR_7g034110 33.333 318 157 9 68 380 65 332 4.47e-30 119
MsG0780038508.01.T01 MTR_1g066830 29.598 348 219 12 51 385 48 382 5.26e-30 119
MsG0780038508.01.T01 MTR_4g039440 29.042 334 203 10 71 385 89 407 1.10e-29 119
MsG0780038508.01.T01 MTR_2g020180 36.715 207 109 5 178 377 13 204 4.20e-29 113
MsG0780038508.01.T01 MTR_7g083740 30.068 296 170 9 102 385 92 362 4.39e-29 116
MsG0780038508.01.T01 MTR_6g022500 30.699 329 196 13 59 377 149 455 4.77e-29 118
MsG0780038508.01.T01 MTR_2g007400 31.837 245 136 6 137 362 147 379 7.06e-29 116
MsG0780038508.01.T01 MTR_5g073370 31.373 306 180 11 67 358 58 347 7.35e-29 116
MsG0780038508.01.T01 MTR_1g107385 33.948 271 141 9 135 385 166 418 8.36e-29 116
MsG0780038508.01.T01 MTR_1g085120 28.818 347 217 9 54 385 135 466 8.50e-29 117
MsG0780038508.01.T01 MTR_2g008310 34.855 241 132 8 131 358 115 343 9.31e-29 116
MsG0780038508.01.T01 MTR_2g008320 33.884 242 135 7 131 358 169 399 1.30e-28 116
MsG0780038508.01.T01 MTR_3g107120 30.973 339 201 12 67 393 77 394 1.35e-28 115
MsG0780038508.01.T01 MTR_1g085120 28.818 347 217 9 54 385 210 541 1.78e-28 117
MsG0780038508.01.T01 MTR_3g099830 30.240 334 189 12 67 385 65 369 4.13e-28 114
MsG0780038508.01.T01 MTR_4g080610 34.894 235 135 4 163 385 124 352 6.58e-28 113
MsG0780038508.01.T01 MTR_1g108800 32.537 335 181 15 70 385 29 337 8.45e-28 112
MsG0780038508.01.T01 MTR_2g005990 31.215 362 201 14 54 385 47 390 1.75e-27 112
MsG0780038508.01.T01 MTR_1g053025 29.268 246 143 7 135 360 129 363 2.81e-27 112
MsG0780038508.01.T01 MTR_5g083890 34.661 251 133 10 130 360 137 376 6.10e-27 112
MsG0780038508.01.T01 MTR_5g058100 30.746 335 205 12 64 385 59 379 6.50e-27 110
MsG0780038508.01.T01 MTR_5g054920 35.000 180 109 3 183 358 2 177 1.83e-26 105
MsG0780038508.01.T01 MTR_3g026920 27.616 344 162 12 101 390 95 405 2.02e-26 109
MsG0780038508.01.T01 MTR_7g056417 47.059 153 63 4 132 271 4 151 2.64e-26 103
MsG0780038508.01.T01 MTR_7g081290 49.612 129 54 5 256 377 121 245 2.85e-26 106
MsG0780038508.01.T01 MTR_5g045510 27.437 277 172 5 86 358 88 339 4.77e-26 108
MsG0780038508.01.T01 MTR_6g011460 30.060 336 194 11 61 380 93 403 1.04e-25 108
MsG0780038508.01.T01 MTR_3g115430 50.505 99 46 2 278 375 16 112 1.85e-25 100
MsG0780038508.01.T01 MTR_7g075820 31.599 269 153 9 131 385 138 389 1.88e-25 107
MsG0780038508.01.T01 MTR_3g107160 32.836 268 159 10 106 364 107 362 2.52e-25 106
MsG0780038508.01.T01 MTR_2g036470 32.618 233 144 6 135 358 135 363 5.26e-25 105
MsG0780038508.01.T01 MTR_7g055943 29.444 360 217 14 55 392 84 428 9.03e-25 105
MsG0780038508.01.T01 MTR_2g007500 32.530 249 141 10 103 333 91 330 1.30e-24 103
MsG0780038508.01.T01 MTR_7g060470 32.258 279 147 14 110 365 107 366 2.96e-24 103
MsG0780038508.01.T01 MTR_5g065100 30.029 343 169 15 58 385 58 344 2.59e-23 101
MsG0780038508.01.T01 MTR_7g007340 33.468 248 137 8 124 358 97 329 3.31e-23 100
MsG0780038508.01.T01 MTR_1g059860 29.391 279 148 9 111 351 44 311 3.41e-23 99.4
MsG0780038508.01.T01 MTR_3g108560 35.366 246 137 9 61 299 67 297 1.30e-22 97.4
MsG0780038508.01.T01 MTR_2g007360 29.304 273 144 10 144 385 115 369 1.88e-22 98.2
MsG0780038508.01.T01 MTR_4g098590 28.713 303 175 13 86 363 73 359 3.18e-21 94.7
MsG0780038508.01.T01 MTR_5g061410 34.000 200 119 5 104 299 77 267 4.99e-21 92.4
MsG0780038508.01.T01 MTR_1g043170 59.091 66 27 0 310 375 10 75 3.73e-20 84.7
MsG0780038508.01.T01 MTR_8g062310 27.536 345 186 13 82 385 102 423 1.07e-19 90.9
MsG0780038508.01.T01 MTR_7g075800 27.362 307 161 13 135 391 141 435 1.32e-19 91.3
MsG0780038508.01.T01 MTR_4g134150 40.571 175 84 9 63 231 56 216 1.72e-19 87.0
MsG0780038508.01.T01 MTR_5g039450 27.187 320 176 13 69 368 63 345 2.46e-19 89.0
MsG0780038508.01.T01 MTR_5g061680 31.449 283 154 13 49 326 47 294 5.56e-19 87.4
MsG0780038508.01.T01 MTR_6g022360 29.119 261 160 9 139 385 154 403 1.21e-18 87.4
MsG0780038508.01.T01 MTR_6g044572 30.268 261 147 11 102 358 123 352 3.09e-18 86.3
MsG0780038508.01.T01 MTR_6g044560 30.268 261 147 11 102 358 299 528 3.17e-18 87.0
MsG0780038508.01.T01 MTR_6g022520 30.496 282 177 7 85 358 10 280 3.63e-18 85.1
MsG0780038508.01.T01 MTR_3g071790 31.351 185 118 6 176 352 19 202 4.48e-18 82.4
MsG0780038508.01.T01 MTR_7g033945 49.091 110 49 5 68 173 65 171 4.23e-17 79.0
MsG0780038508.01.T01 MTR_3g107940 39.669 121 61 2 260 377 88 199 5.88e-17 81.6
MsG0780038508.01.T01 MTR_3g110010 34.286 175 100 6 135 302 220 386 7.13e-17 83.2
MsG0780038508.01.T01 MTR_3g110010 37.313 134 77 4 68 201 67 193 9.88e-13 70.1
MsG0780038508.01.T01 MTR_7g022060 29.110 292 150 11 98 383 83 323 8.16e-17 81.3
MsG0780038508.01.T01 MTR_1g085120 29.231 260 157 7 54 299 222 468 1.01e-16 82.0
MsG0780038508.01.T01 MTR_2g007090 31.250 208 107 6 163 360 87 268 1.45e-16 80.1
MsG0780038508.01.T01 MTR_7g007370 32.203 236 140 7 135 360 94 319 2.26e-16 80.1
MsG0780038508.01.T01 MTR_5g073340 32.174 230 134 8 114 327 77 300 2.84e-16 79.3
MsG0780038508.01.T01 MTR_7g021930 29.110 292 150 11 98 383 83 323 6.00e-16 78.6
MsG0780038508.01.T01 MTR_7g021970 29.110 292 150 11 98 383 83 323 6.00e-16 78.6
MsG0780038508.01.T01 MTR_3g095630 32.653 147 87 4 214 358 15 151 6.55e-16 75.9
MsG0780038508.01.T01 MTR_2g007550 29.442 197 103 7 214 391 157 336 7.04e-16 78.6
MsG0780038508.01.T01 MTR_1g108820 29.762 252 152 8 67 303 61 302 2.37e-15 77.0
MsG0780038508.01.T01 MTR_3g094980 32.759 116 68 2 278 383 33 148 8.82e-15 72.4
MsG0780038508.01.T01 MTR_3g108140 44.872 78 42 1 310 386 67 144 9.88e-15 72.4
MsG0780038508.01.T01 MTR_1377s0010 34.234 111 66 3 259 367 31 136 2.57e-14 70.1
MsG0780038508.01.T01 MTR_7g056280 28.507 221 117 9 187 385 367 568 4.85e-14 73.9
MsG0780038508.01.T01 MTR_5g024060 30.935 139 93 2 255 393 76 211 5.37e-14 71.6
MsG0780038508.01.T01 MTR_3g106960 38.532 109 60 4 254 358 66 171 2.61e-13 69.3
MsG0780038508.01.T01 MTR_7g022105 27.138 269 168 8 121 373 152 408 4.63e-13 70.5
MsG0780038508.01.T01 MTR_2g007530 31.618 136 69 3 255 390 226 337 7.43e-13 69.3
MsG0780038508.01.T01 MTR_5g080710 25.685 292 163 8 123 363 137 425 2.89e-12 68.2
MsG0780038508.01.T01 MTR_3g113785 37.755 98 44 3 260 348 3 92 6.77e-12 62.0
MsG0780038508.01.T01 MTR_7g021950 25.000 288 178 7 121 387 151 421 9.86e-12 66.6
MsG0780038508.01.T01 MTR_3g107140 30.366 191 118 6 19 203 7 188 1.03e-11 64.3
MsG0780038508.01.T01 MTR_5g076150 29.108 213 112 8 139 323 26 227 1.09e-11 64.7
MsG0780038508.01.T01 MTR_7g021990 25.000 288 178 7 121 387 151 421 1.39e-11 66.2
MsG0780038508.01.T01 MTR_8g062010 23.747 379 212 15 66 385 65 425 3.24e-11 65.1
MsG0780038508.01.T01 MTR_5g073400 41.880 117 52 5 86 198 7 111 9.03e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038508.01.T01 AT5G56370 25.676 370 215 12 68 386 57 417 2.87e-18 86.3
MsG0780038508.01.T01 AT5G56370 25.676 370 215 12 68 386 57 417 2.87e-18 86.3
MsG0780038508.01.T01 AT4G10400 26.901 342 196 16 86 385 75 404 4.85e-13 70.5
MsG0780038508.01.T01 AT4G10400 26.901 342 196 16 86 385 75 404 4.85e-13 70.5
MsG0780038508.01.T01 AT4G10400 26.901 342 196 16 86 385 75 404 4.85e-13 70.5
MsG0780038508.01.T01 AT5G56370 29.044 272 146 9 68 301 57 319 1.10e-11 65.9
MsG0780038508.01.T01 AT4G00160 27.839 273 136 12 135 354 136 400 4.97e-11 64.7

Find 88 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTTAAATGCATGGAATT+TGG 0.120045 7:-47807017 MsG0780038508.01.T01:CDS
TAGTAGGTAAATTGACTTTA+AGG 0.163602 7:+47807800 None:intergenic
AAGAGGGAGTTGTGGATAAT+TGG 0.248533 7:-47807100 MsG0780038508.01.T01:CDS
AGCTGGGTTGAAAGGCATTC+TGG 0.263569 7:+47807059 None:intergenic
GATCAATCCCGACCGAAAAT+AGG 0.278633 7:-47808168 MsG0780038508.01.T01:CDS
AATGATTAGATTTCGAAGTT+TGG 0.300529 7:+47807236 None:intergenic
TGAAAGTATGTGATGTATAA+TGG 0.309816 7:+47808126 None:intergenic
GCTTTAAATGCATGGAATTT+GGG 0.312871 7:-47807016 MsG0780038508.01.T01:CDS
TTTCGATGTTCTTGAAGATT+TGG 0.315877 7:-47807659 MsG0780038508.01.T01:CDS
TTTCTCATTTGATGATTAGT+AGG 0.320115 7:-47807516 MsG0780038508.01.T01:CDS
CTCAAAACATGTTCGCTTGA+AGG 0.330623 7:-47807038 MsG0780038508.01.T01:CDS
ATGATTAGATTTCGAAGTTT+GGG 0.348309 7:+47807237 None:intergenic
AAATAGGATTAGCGCATTAC+CGG 0.348768 7:-47808152 MsG0780038508.01.T01:CDS
CTAGAGGAATTGCAAATAGA+TGG 0.356981 7:-47807610 MsG0780038508.01.T01:CDS
ATGTTTGCTTTAATCAAATT+CGG 0.361688 7:+47807538 None:intergenic
AGGGAAGTCACTTGGTTTAG+TGG 0.375224 7:-47807578 MsG0780038508.01.T01:CDS
GGAGGACGCAAATCTGAAAT+AGG 0.381672 7:+47807987 None:intergenic
GCTTGTTGCAGCAGAGAGTT+TGG 0.388243 7:+47808093 None:intergenic
TATTTGCAATTCCTCTAGAA+TGG 0.389646 7:+47807615 None:intergenic
GAAATAGGAGTCAGTAGTTC+GGG 0.390414 7:+47808002 None:intergenic
GATTTGGTAAAGCTTCTATC+AGG 0.394092 7:-47807643 MsG0780038508.01.T01:CDS
ATGAAGATTTCATTAACATT+TGG 0.395120 7:+47806901 None:intergenic
TGTAACTCTTATTTAAATGA+TGG 0.404168 7:-47806858 MsG0780038508.01.T01:CDS
ATTGATATCGTGTGAGTAAT+GGG 0.412796 7:+47807897 None:intergenic
TTTCATTAACATTTGGTACT+TGG 0.417644 7:+47806908 None:intergenic
GAAAGTATGTGATGTATAAT+GGG 0.421631 7:+47808127 None:intergenic
CTTGTCGTTCTTCATTTGAA+AGG 0.423191 7:-47807757 MsG0780038508.01.T01:CDS
TTGTGTTTACTTTGCTTCGC+AGG 0.442108 7:-47807483 MsG0780038508.01.T01:CDS
TGAAATAGGAGTCAGTAGTT+CGG 0.443462 7:+47808001 None:intergenic
AGGCATTCTGGAATAATTGT+TGG 0.444056 7:+47807071 None:intergenic
GTTGCAGCAGAGAGTTTGGT+TGG 0.462655 7:+47808097 None:intergenic
ATTTGCAATTCCTCTAGAAT+GGG 0.471933 7:+47807616 None:intergenic
TGAATTGTTGACAGAAGTCT+AGG 0.474487 7:-47807127 MsG0780038508.01.T01:intron
TTGTCGTTCTTCATTTGAAA+GGG 0.480804 7:-47807756 MsG0780038508.01.T01:CDS
CTACAGCTGAAAATACTAGT+AGG 0.492188 7:+47807784 None:intergenic
TGCTTCAACGCTGTGAGAAG+TGG 0.492985 7:-47807290 MsG0780038508.01.T01:CDS
TAGGATTGAAGAGGGAGTTG+TGG 0.495134 7:-47807108 MsG0780038508.01.T01:CDS
GTACTTGGCATTTAAATCTA+CGG 0.498335 7:+47806923 None:intergenic
TGTATCTCACTGCAGGACAT+AGG 0.498962 7:-47807366 MsG0780038508.01.T01:intron
TTATTTAAATGATGGAAGGT+AGG 0.499013 7:-47806850 None:intergenic
GATTGATATCGTGTGAGTAA+TGG 0.501484 7:+47807896 None:intergenic
TTGCAGCAGAGAGTTTGGTT+GGG 0.508486 7:+47808098 None:intergenic
CAAGCATCCTTTCAAAGAGA+TGG 0.510650 7:-47808075 MsG0780038508.01.T01:CDS
TTGCAAATAGATGGGGTACA+TGG 0.512169 7:-47807601 MsG0780038508.01.T01:CDS
TTTAAGGAAAACCATGCTGC+TGG 0.513860 7:+47807816 None:intergenic
GTGGTTTCCATCTCTTTGAA+AGG 0.514966 7:+47808068 None:intergenic
TAGAGGAATTGCAAATAGAT+GGG 0.517788 7:-47807609 MsG0780038508.01.T01:CDS
TGCTGGATATTTCGAGATTG+AGG 0.518337 7:+47807833 None:intergenic
GGCATTTAAATCTACGGAAG+AGG 0.520093 7:+47806929 None:intergenic
TTCTCATTTGATGATTAGTA+GGG 0.520724 7:-47807515 MsG0780038508.01.T01:CDS
GCTTGAAGGCTTTAAATGCA+TGG 0.521098 7:-47807024 MsG0780038508.01.T01:CDS
ATGTGATGTATAATGGGATC+CGG 0.523772 7:+47808133 None:intergenic
TATTTAAATGATGGAAGGTA+GGG 0.533136 7:-47806849 None:intergenic
GTACATGGAGGGAAGTCACT+TGG 0.536365 7:-47807586 MsG0780038508.01.T01:CDS
GAAGTTTGGGACAACGTTTG+AGG 0.540821 7:+47807250 None:intergenic
GGGAGTTGTGGATAATTGGA+TGG 0.542611 7:-47807096 MsG0780038508.01.T01:CDS
ACAGAAGTCTAGGATTGAAG+AGG 0.548692 7:-47807117 MsG0780038508.01.T01:intron
AACATTTGAGGTGGAATGAT+CGG 0.555465 7:+47807932 None:intergenic
TCGAAATCTAATCATTCATG+AGG 0.557538 7:-47807229 MsG0780038508.01.T01:intron
CAGAAGTCTAGGATTGAAGA+GGG 0.558293 7:-47807116 MsG0780038508.01.T01:CDS
TAATGAAATCTTCATTCACA+AGG 0.564688 7:-47806893 MsG0780038508.01.T01:CDS
ACTCTTATTTAAATGATGGA+AGG 0.568656 7:-47806854 MsG0780038508.01.T01:CDS
TGTGTTTACTTTGCTTCGCA+GGG 0.569835 7:-47807482 MsG0780038508.01.T01:CDS
TAGAAGCTGGTGAACATTTG+AGG 0.571282 7:+47807920 None:intergenic
TCATTTGATGATTAGTAGGG+AGG 0.572505 7:-47807512 MsG0780038508.01.T01:CDS
TTTGAGGTGGAATGATCGGA+TGG 0.579598 7:+47807936 None:intergenic
TTGTCGTCGAAGCGGAGAGT+CGG 0.585099 7:+47808031 None:intergenic
GTTTCAAAATCTAATCCACA+TGG 0.586722 7:-47807325 MsG0780038508.01.T01:CDS
AAATAGATGGGGTACATGGA+GGG 0.592790 7:-47807597 MsG0780038508.01.T01:CDS
CAAATAGATGGGGTACATGG+AGG 0.601670 7:-47807598 MsG0780038508.01.T01:CDS
AATGAAATCTTCATTCACAA+GGG 0.611924 7:-47806892 MsG0780038508.01.T01:CDS
GATTCCTCCTCGTTGAGCAG+AGG 0.618410 7:+47807861 None:intergenic
ATCAGGATGTCCCATTCTAG+AGG 0.622759 7:-47807626 MsG0780038508.01.T01:CDS
TGTGAGTAATGGGTAGAAGC+TGG 0.640754 7:+47807907 None:intergenic
GGAAGGCTTTGTCGTCGAAG+CGG 0.646835 7:+47808023 None:intergenic
AAGCTGGTGAACATTTGAGG+TGG 0.654046 7:+47807923 None:intergenic
TTGAGGTGGAATGATCGGAT+GGG 0.654910 7:+47807937 None:intergenic
AGAGGAATTGCAAATAGATG+GGG 0.659055 7:-47807608 MsG0780038508.01.T01:CDS
CTTTCAAATGAAGAACGACA+AGG 0.659260 7:+47807758 None:intergenic
TAGGAGTCAGTAGTTCGGGA+AGG 0.664606 7:+47808006 None:intergenic
TCTCGAAATATCCAGCAGCA+TGG 0.675557 7:-47807827 MsG0780038508.01.T01:CDS
GATGACTCGTAAGAGAAAGA+CGG 0.678545 7:+47807966 None:intergenic
TTTCAAATGAAGAACGACAA+GGG 0.680011 7:+47807759 None:intergenic
CAAAGAGATGGAAACCACTG+TGG 0.681506 7:-47808063 MsG0780038508.01.T01:CDS
TATGCCTCTGCTCAACGAGG+AGG 0.681876 7:-47807865 MsG0780038508.01.T01:CDS
GTCTATGCCTCTGCTCAACG+AGG 0.693871 7:-47807868 MsG0780038508.01.T01:CDS
GTCGGAACTGAGAGCCACAG+TGG 0.710460 7:+47808049 None:intergenic
GACTCGTAAGAGAAAGACGG+AGG 0.711358 7:+47807969 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTCATTGAAATATTTAT+TGG + Chr7:47807630-47807649 None:intergenic 10.0%
!! TTTGTTAATAAAAAATAAAG+AGG + Chr7:47807888-47807907 None:intergenic 10.0%
!! AAAAATTATGTGTCTAATGT+TGG - Chr7:47807582-47807601 MsG0780038508.01.T01:CDS 20.0%
!! ATGAAGATTTCATTAACATT+TGG + Chr7:47808142-47808161 None:intergenic 20.0%
!! ATGTTTGCTTTAATCAAATT+CGG + Chr7:47807505-47807524 None:intergenic 20.0%
!!! TATTGTCAATTATTTCTCTT+TGG - Chr7:47807840-47807859 MsG0780038508.01.T01:CDS 20.0%
!!! TGTAACTCTTATTTAAATGA+TGG - Chr7:47808182-47808201 MsG0780038508.01.T01:CDS 20.0%
! AATGAAATCTTCATTCACAA+GGG - Chr7:47808148-47808167 MsG0780038508.01.T01:CDS 25.0%
! AATGATTAGATTTCGAAGTT+TGG + Chr7:47807807-47807826 None:intergenic 25.0%
! ATGATTAGATTTCGAAGTTT+GGG + Chr7:47807806-47807825 None:intergenic 25.0%
! TAATGAAATCTTCATTCACA+AGG - Chr7:47808147-47808166 MsG0780038508.01.T01:CDS 25.0%
! TTTCATTAACATTTGGTACT+TGG + Chr7:47808135-47808154 None:intergenic 25.0%
!! ACTCTTATTTAAATGATGGA+AGG - Chr7:47808186-47808205 MsG0780038508.01.T01:CDS 25.0%
!! GAAAGTATGTGATGTATAAT+GGG + Chr7:47806916-47806935 None:intergenic 25.0%
!! TAGTAGGTAAATTGACTTTA+AGG + Chr7:47807243-47807262 None:intergenic 25.0%
!! TGAAAGTATGTGATGTATAA+TGG + Chr7:47806917-47806936 None:intergenic 25.0%
!! TTCTCATTTGATGATTAGTA+GGG - Chr7:47807525-47807544 MsG0780038508.01.T01:CDS 25.0%
!! TTTCTCATTTGATGATTAGT+AGG - Chr7:47807524-47807543 MsG0780038508.01.T01:CDS 25.0%
!!! GATTTTGAAACAAAGGAAAA+TGG + Chr7:47807706-47807725 None:intergenic 25.0%
!!! TGGATTAGATTTTGAAACAA+AGG + Chr7:47807713-47807732 None:intergenic 25.0%
AAAGAGGTGAAAAAATGTTG+AGG + Chr7:47807872-47807891 None:intergenic 30.0%
ATTGATATCGTGTGAGTAAT+GGG + Chr7:47807146-47807165 None:intergenic 30.0%
CCTAAAAACTCTTCATCTAA+GGG - Chr7:47807351-47807370 MsG0780038508.01.T01:CDS 30.0%
GTACTTGGCATTTAAATCTA+CGG + Chr7:47808120-47808139 None:intergenic 30.0%
GTTTCAAAATCTAATCCACA+TGG - Chr7:47807715-47807734 MsG0780038508.01.T01:CDS 30.0%
TAGAGGAATTGCAAATAGAT+GGG - Chr7:47807431-47807450 MsG0780038508.01.T01:intron 30.0%
TCCTAAAAACTCTTCATCTA+AGG - Chr7:47807350-47807369 MsG0780038508.01.T01:CDS 30.0%
TCGAAATCTAATCATTCATG+AGG - Chr7:47807811-47807830 MsG0780038508.01.T01:CDS 30.0%
TTGTCGTTCTTCATTTGAAA+GGG - Chr7:47807284-47807303 MsG0780038508.01.T01:CDS 30.0%
TTTCAAATGAAGAACGACAA+GGG + Chr7:47807284-47807303 None:intergenic 30.0%
TTTCGATGTTCTTGAAGATT+TGG - Chr7:47807381-47807400 MsG0780038508.01.T01:intron 30.0%
! GCTTTAAATGCATGGAATTT+GGG - Chr7:47808024-47808043 MsG0780038508.01.T01:CDS 30.0%
! TAATGCGCTAATCCTATTTT+CGG + Chr7:47806887-47806906 None:intergenic 30.0%
! TATTTGCAATTCCTCTAGAA+TGG + Chr7:47807428-47807447 None:intergenic 30.0%
!! ATTTGCAATTCCTCTAGAAT+GGG + Chr7:47807427-47807446 None:intergenic 30.0%
AGAGGAATTGCAAATAGATG+GGG - Chr7:47807432-47807451 MsG0780038508.01.T01:intron 35.0%
CTACAGCTGAAAATACTAGT+AGG + Chr7:47807259-47807278 None:intergenic 35.0%
CTAGAGGAATTGCAAATAGA+TGG - Chr7:47807430-47807449 MsG0780038508.01.T01:intron 35.0%
CTTGTCGTTCTTCATTTGAA+AGG - Chr7:47807283-47807302 MsG0780038508.01.T01:CDS 35.0%
CTTTCAAATGAAGAACGACA+AGG + Chr7:47807285-47807304 None:intergenic 35.0%
GATTGATATCGTGTGAGTAA+TGG + Chr7:47807147-47807166 None:intergenic 35.0%
GCATAAAAAGAAGTTCCATG+TGG + Chr7:47807733-47807752 None:intergenic 35.0%
TGAAATAGGAGTCAGTAGTT+CGG + Chr7:47807042-47807061 None:intergenic 35.0%
TGAATTGTTGACAGAAGTCT+AGG - Chr7:47807913-47807932 MsG0780038508.01.T01:CDS 35.0%
! AAATAGGATTAGCGCATTAC+CGG - Chr7:47806888-47806907 MsG0780038508.01.T01:CDS 35.0%
! AACATTTGAGGTGGAATGAT+CGG + Chr7:47807111-47807130 None:intergenic 35.0%
! ATGTGATGTATAATGGGATC+CGG + Chr7:47806910-47806929 None:intergenic 35.0%
! CCCTTAGATGAAGAGTTTTT+AGG + Chr7:47807354-47807373 None:intergenic 35.0%
! CTACTAGTATTTTCAGCTGT+AGG - Chr7:47807257-47807276 MsG0780038508.01.T01:CDS 35.0%
! GATTTGGTAAAGCTTCTATC+AGG - Chr7:47807397-47807416 MsG0780038508.01.T01:intron 35.0%
! TAACTTTTGTATCTCACTGC+AGG - Chr7:47807667-47807686 MsG0780038508.01.T01:CDS 35.0%
! TCATTTGATGATTAGTAGGG+AGG - Chr7:47807528-47807547 MsG0780038508.01.T01:CDS 35.0%
!! AGATGAAGAGTTTTTAGGAG+AGG + Chr7:47807349-47807368 None:intergenic 35.0%
!! AGGCATTCTGGAATAATTGT+TGG + Chr7:47807972-47807991 None:intergenic 35.0%
!! GATGAAGAGTTTTTAGGAGA+GGG + Chr7:47807348-47807367 None:intergenic 35.0%
!! GGCTTTAAATGCATGGAATT+TGG - Chr7:47808023-47808042 MsG0780038508.01.T01:CDS 35.0%
AAATAGATGGGGTACATGGA+GGG - Chr7:47807443-47807462 MsG0780038508.01.T01:intron 40.0%
AAGAGGGAGTTGTGGATAAT+TGG - Chr7:47807940-47807959 MsG0780038508.01.T01:CDS 40.0%
CAAGCATCCTTTCAAAGAGA+TGG - Chr7:47806965-47806984 MsG0780038508.01.T01:CDS 40.0%
CTCAAAACATGTTCGCTTGA+AGG - Chr7:47808002-47808021 MsG0780038508.01.T01:CDS 40.0%
GAAATAGGAGTCAGTAGTTC+GGG + Chr7:47807041-47807060 None:intergenic 40.0%
GATGACTCGTAAGAGAAAGA+CGG + Chr7:47807077-47807096 None:intergenic 40.0%
GGCATTTAAATCTACGGAAG+AGG + Chr7:47808114-47808133 None:intergenic 40.0%
GTGGTTTCCATCTCTTTGAA+AGG + Chr7:47806975-47806994 None:intergenic 40.0%
TAGAAGCTGGTGAACATTTG+AGG + Chr7:47807123-47807142 None:intergenic 40.0%
TGCTGGATATTTCGAGATTG+AGG + Chr7:47807210-47807229 None:intergenic 40.0%
TGTGTTTACTTTGCTTCGCA+GGG - Chr7:47807558-47807577 MsG0780038508.01.T01:CDS 40.0%
TTGCAAATAGATGGGGTACA+TGG - Chr7:47807439-47807458 MsG0780038508.01.T01:intron 40.0%
TTGTGTTTACTTTGCTTCGC+AGG - Chr7:47807557-47807576 MsG0780038508.01.T01:CDS 40.0%
TTTAAGGAAAACCATGCTGC+TGG + Chr7:47807227-47807246 None:intergenic 40.0%
! ACAGAAGTCTAGGATTGAAG+AGG - Chr7:47807923-47807942 MsG0780038508.01.T01:CDS 40.0%
! CAGAAGTCTAGGATTGAAGA+GGG - Chr7:47807924-47807943 MsG0780038508.01.T01:CDS 40.0%
!! AAGCGAACATGTTTTGAGCT+GGG + Chr7:47808000-47808019 None:intergenic 40.0%
!! ATGTTTTGAGCTGGGTTGAA+AGG + Chr7:47807992-47808011 None:intergenic 40.0%
!! GCTTGAAGGCTTTAAATGCA+TGG - Chr7:47808016-47808035 MsG0780038508.01.T01:CDS 40.0%
AAGCTGGTGAACATTTGAGG+TGG + Chr7:47807120-47807139 None:intergenic 45.0%
AGGGAAGTCACTTGGTTTAG+TGG - Chr7:47807462-47807481 MsG0780038508.01.T01:CDS 45.0%
ATCAGGATGTCCCATTCTAG+AGG - Chr7:47807414-47807433 MsG0780038508.01.T01:intron 45.0%
CAAAGAGATGGAAACCACTG+TGG - Chr7:47806977-47806996 MsG0780038508.01.T01:CDS 45.0%
CAAATAGATGGGGTACATGG+AGG - Chr7:47807442-47807461 MsG0780038508.01.T01:intron 45.0%
GATCAATCCCGACCGAAAAT+AGG - Chr7:47806872-47806891 MsG0780038508.01.T01:CDS 45.0%
GGGAGTTGTGGATAATTGGA+TGG - Chr7:47807944-47807963 MsG0780038508.01.T01:CDS 45.0%
TAGGATTGAAGAGGGAGTTG+TGG - Chr7:47807932-47807951 MsG0780038508.01.T01:CDS 45.0%
TCTCGAAATATCCAGCAGCA+TGG - Chr7:47807213-47807232 MsG0780038508.01.T01:intron 45.0%
TGTATCTCACTGCAGGACAT+AGG - Chr7:47807674-47807693 MsG0780038508.01.T01:CDS 45.0%
TTGAGGTGGAATGATCGGAT+GGG + Chr7:47807106-47807125 None:intergenic 45.0%
TTGCAGCAGAGAGTTTGGTT+GGG + Chr7:47806945-47806964 None:intergenic 45.0%
! CGCTAATCCTATTTTCGGTC+GGG + Chr7:47806882-47806901 None:intergenic 45.0%
! GAAGTTTGGGACAACGTTTG+AGG + Chr7:47807793-47807812 None:intergenic 45.0%
! GCGCTAATCCTATTTTCGGT+CGG + Chr7:47806883-47806902 None:intergenic 45.0%
! GGAGGACGCAAATCTGAAAT+AGG + Chr7:47807056-47807075 None:intergenic 45.0%
! TTTGAGGTGGAATGATCGGA+TGG + Chr7:47807107-47807126 None:intergenic 45.0%
!! CAAGCGAACATGTTTTGAGC+TGG + Chr7:47808001-47808020 None:intergenic 45.0%
!! TGTGAGTAATGGGTAGAAGC+TGG + Chr7:47807136-47807155 None:intergenic 45.0%
GACTCGTAAGAGAAAGACGG+AGG + Chr7:47807074-47807093 None:intergenic 50.0%
GTACATGGAGGGAAGTCACT+TGG - Chr7:47807454-47807473 MsG0780038508.01.T01:intron 50.0%
GTTGCAGCAGAGAGTTTGGT+TGG + Chr7:47806946-47806965 None:intergenic 50.0%
TAGGAGTCAGTAGTTCGGGA+AGG + Chr7:47807037-47807056 None:intergenic 50.0%
TGCTTCAACGCTGTGAGAAG+TGG - Chr7:47807750-47807769 MsG0780038508.01.T01:CDS 50.0%
! GCTTGTTGCAGCAGAGAGTT+TGG + Chr7:47806950-47806969 None:intergenic 50.0%
!! AGCTGGGTTGAAAGGCATTC+TGG + Chr7:47807984-47808003 None:intergenic 50.0%
GATTCCTCCTCGTTGAGCAG+AGG + Chr7:47807182-47807201 None:intergenic 55.0%
GTCTATGCCTCTGCTCAACG+AGG - Chr7:47807172-47807191 MsG0780038508.01.T01:intron 55.0%
TATGCCTCTGCTCAACGAGG+AGG - Chr7:47807175-47807194 MsG0780038508.01.T01:intron 55.0%
TTGTCGTCGAAGCGGAGAGT+CGG + Chr7:47807012-47807031 None:intergenic 55.0%
! GGAAGGCTTTGTCGTCGAAG+CGG + Chr7:47807020-47807039 None:intergenic 55.0%
GTCGGAACTGAGAGCCACAG+TGG + Chr7:47806994-47807013 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 47806851 47808211 47806851 ID=MsG0780038508.01;Name=MsG0780038508.01
Chr7 mRNA 47806851 47808211 47806851 ID=MsG0780038508.01.T01;Parent=MsG0780038508.01;Name=MsG0780038508.01.T01;_AED=0.30;_eAED=0.40;_QI=0|0|0|1|1|1|3|0|393
Chr7 exon 47807459 47808211 47807459 ID=MsG0780038508.01.T01:exon:5039;Parent=MsG0780038508.01.T01
Chr7 exon 47807230 47807373 47807230 ID=MsG0780038508.01.T01:exon:5038;Parent=MsG0780038508.01.T01
Chr7 exon 47806851 47807135 47806851 ID=MsG0780038508.01.T01:exon:5037;Parent=MsG0780038508.01.T01
Chr7 CDS 47807459 47808211 47807459 ID=MsG0780038508.01.T01:cds;Parent=MsG0780038508.01.T01
Chr7 CDS 47807230 47807373 47807230 ID=MsG0780038508.01.T01:cds;Parent=MsG0780038508.01.T01
Chr7 CDS 47806851 47807135 47806851 ID=MsG0780038508.01.T01:cds;Parent=MsG0780038508.01.T01
Gene Sequence

>MsG0780038508.01.T01

TTGAGTTTTGAAATATGTCACGATCAATCCCGACCGAAAATAGGATTAGCGCATTACCGGATCCCATTATACATCACATACTTTCATTTCTCCCAACCAAACTCTCTGCTGCAACAAGCATCCTTTCAAAGAGATGGAAACCACTGTGGCTCTCAGTTCCGACTCTCCGCTTCGACGACAAAGCCTTCCCGAACTACTGACTCCTATTTCAGATTTGCGTCCTCCGTCTTTCTCTTACGAGTCATCACGTTACCCATCCGATCATTCCACCTCAAATGTTCACCAGCTTCTACCCATTACTCACACGATATCAATCGATTTGTCTATGCCTCTGCTCAACGAGGAGGAATCGAGAGCCTCAATCTCGAAATATCCAGCAGCATGGTTTTCCTTAAAGTCAATTTACCTACTAGTATTTTCAGCTGTAGGACCCTTGTCGTTCTTCATTTGAAAGGGTTAAGAGTGAATGATCTTTCTCAATTGATTGTAGATTTCCCTCTCCTAAAAACTCTTCATCTAAGGGTTATGCGTTTCGATGTTCTTGAAGATTTGGTAAAGCTTCTATCAGGATGTCCCATTCTAGAGGAATTGCAAATAGATGGGGTACATGGAGGGAAGTCACTTGGTTTAGTGGAAAATTCTCAAAGCTTACCGAATTTGATTAAAGCAAACATTTCTCATTTGATGATTAGTAGGGAGGCGAGTGTTGTGTTTACTTTGCTTCGCAGGGCAAAAATTATGTGTCTAATGTTGGACATAGGTTATATCCATTTTCCTTTGTTTCAAAATCTAATCCACATGGAACTTCTTTTTATGCTTCAACGCTGTGAGAAGTGGAAGTGCGTTATAGAAGTCCTCAAACGTTGTCCCAAACTTCGAAATCTAATCATTCATGAGAAGTCTAGGATTGAAGAGGGAGTTGTGGATAATTGGATGGATCCAACAATTATTCCAGAATGCCTTTCAACCCAGCTCAAAACATGTTCGCTTGAAGGCTTTAAATGCATGGAATTTGGGATTCAATTTGCAAAATATATTATGCAGAATTCAAAAGTACTGAACATCATGTCAATCAAGAGTGCCTCTTCCGTAGATTTAAATGCCAAGTACCAAATGTTAATGAAATCTTCATTCACAAGGGCTTCTACAAAATGTAACTCTTATTTAAATGATGGAAGGTAG
Protein sequence

>MsG0780038508.01.T01

LSFEICHDQSRPKIGLAHYRIPLYITYFHFSQPNSLLQQASFQRDGNHCGSQFRLSASTTKPSRTTDSYFRFASSVFLLRVITLPIRSFHLKCSPASTHYSHDINRFVYASAQRGGIESLNLEISSSMVFLKVNLPTSIFSCRTLVVLHLKGLRVNDLSQLIVDFPLLKTLHLRVMRFDVLEDLVKLLSGCPILEELQIDGVHGGKSLGLVENSQSLPNLIKANISHLMISREASVVFTLLRRAKIMCLMLDIGYIHFPLFQNLIHMELLFMLQRCEKWKCVIEVLKRCPKLRNLIIHEKSRIEEGVVDNWMDPTIIPECLSTQLKTCSLEGFKCMEFGIQFAKYIMQNSKVLNIMSIKSASSVDLNAKYQMLMKSSFTRASTKCNSYLNDGR*