Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009317.01.T01 | XP_024630669.2 | 98.81 | 84 | 1 | 0 | 1 | 84 | 93 | 176 | 1.12E-55 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009317.01.T01 | A0A396JWR6 | 95.238 | 84 | 4 | 0 | 1 | 84 | 15 | 98 | 1.15e-54 | 173 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280009317.01 | MsG0280009646.01 | 0.802843 | 4.627684e-49 | 2.290525e-46 |
| MsG0280009317.01 | MsG0480022259.01 | 0.823896 | 1.082000e-53 | 9.438099e-51 |
| MsG0280009317.01 | MsG0480022262.01 | 0.819098 | 1.387776e-52 | 1.057935e-49 |
| MsG0280009317.01 | MsG0580027766.01 | 0.802714 | 4.921270e-49 | 2.427743e-46 |
| MsG0280009317.01 | MsG0680032158.01 | 0.816319 | 5.878140e-52 | 4.151896e-49 |
| MsG0280009317.01 | MsG0780036080.01 | 0.813909 | 2.016062e-51 | 1.333447e-48 |
| MsG0280009317.01 | MsG0880043812.01 | 0.808430 | 3.109987e-50 | 1.777878e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009317.01.T01 | MTR_5g077900 | 95.238 | 84 | 4 | 0 | 1 | 84 | 143 | 226 | 9.93e-57 | 174 |
| MsG0280009317.01.T01 | MTR_0002s1270 | 95.238 | 84 | 4 | 0 | 1 | 84 | 317 | 400 | 2.10e-55 | 176 |
| MsG0280009317.01.T01 | MTR_7g046380 | 97.260 | 73 | 2 | 0 | 1 | 73 | 325 | 397 | 7.76e-48 | 156 |
| MsG0280009317.01.T01 | MTR_0057s0200 | 98.305 | 59 | 1 | 0 | 26 | 84 | 1 | 59 | 2.39e-39 | 124 |
| MsG0280009317.01.T01 | MTR_4g066320 | 84.127 | 63 | 9 | 1 | 23 | 84 | 59 | 121 | 1.00e-34 | 115 |
| MsG0280009317.01.T01 | MTR_3g028430 | 74.074 | 54 | 5 | 1 | 31 | 84 | 15 | 59 | 1.67e-21 | 79.7 |
| MsG0280009317.01.T01 | MTR_6g010930 | 57.333 | 75 | 0 | 1 | 2 | 76 | 10 | 52 | 2.61e-20 | 76.6 |
| MsG0280009317.01.T01 | MTR_4g066300 | 88.235 | 34 | 4 | 0 | 22 | 55 | 5 | 38 | 1.09e-15 | 65.1 |
| MsG0280009317.01.T01 | MTR_2g086200 | 64.583 | 48 | 17 | 0 | 35 | 82 | 185 | 232 | 2.10e-15 | 68.2 |
| MsG0280009317.01.T01 | MTR_8g017000 | 38.824 | 85 | 50 | 1 | 2 | 84 | 605 | 689 | 1.75e-14 | 67.0 |
| MsG0280009317.01.T01 | MTR_2g037270 | 38.824 | 85 | 50 | 1 | 2 | 84 | 605 | 689 | 2.51e-14 | 66.6 |
| MsG0280009317.01.T01 | MTR_2g092960 | 37.179 | 78 | 49 | 0 | 4 | 81 | 1139 | 1216 | 2.85e-14 | 66.6 |
| MsG0280009317.01.T01 | MTR_0462s0020 | 37.037 | 81 | 49 | 1 | 2 | 80 | 211 | 291 | 7.09e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCCATTAGGTTGCTTTCT+TGG | 0.248489 | 2:+50227782 | None:intergenic |
| TGGTTAACACAGAGCTTTAG+AGG | 0.329159 | 2:-50227957 | None:intergenic |
| GCAATAGAAAGAAGGCTAAA+TGG | 0.337305 | 2:-50227916 | MsG0280009317.01.T01:CDS |
| AGAGGTTCATCAACTTGCTC+AGG | 0.406730 | 2:-50227939 | MsG0280009317.01.T01:CDS |
| TATCCACAATCATTGCCATT+AGG | 0.411955 | 2:+50227770 | None:intergenic |
| TCTTGACTTGTATAACGCTT+AGG | 0.431501 | 2:-50227515 | None:intergenic |
| CTGCTAATATTACTAAGTCT+TGG | 0.472781 | 2:-50227715 | MsG0280009317.01.T01:CDS |
| TCATCTTCAGTTAATGTCGT+TGG | 0.484681 | 2:+50227574 | None:intergenic |
| GTATGATTTGCGAAACCAAT+GGG | 0.494818 | 2:-50227548 | MsG0280009317.01.T01:CDS |
| TAGCCAAGAAAGCAACCTAA+TGG | 0.495668 | 2:-50227785 | MsG0280009317.01.T01:CDS |
| CAACCTAATGGCAATGATTG+TGG | 0.497368 | 2:-50227773 | MsG0280009317.01.T01:CDS |
| CAAGAAAACAAGTTGCCCAT+TGG | 0.504204 | 2:+50227533 | None:intergenic |
| TGCTCAGGGCAATAGAAAGA+AGG | 0.548493 | 2:-50227924 | MsG0280009317.01.T01:CDS |
| GAGGTTCATCAACTTGCTCA+GGG | 0.558482 | 2:-50227938 | MsG0280009317.01.T01:CDS |
| TGTATGATTTGCGAAACCAA+TGG | 0.592485 | 2:-50227549 | MsG0280009317.01.T01:CDS |
| TAATATTACTAAGTCTTGGA+TGG | 0.604101 | 2:-50227711 | MsG0280009317.01.T01:CDS |
| TATTACTAAGTCTTGGATGG+AGG | 0.643493 | 2:-50227708 | MsG0280009317.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAGGATATGTTTATATT+TGG | + | Chr2:50227822-50227841 | None:intergenic | 15.0% |
| !! | ATGAACTCACTATATAAAAA+TGG | - | Chr2:50227644-50227663 | MsG0280009317.01.T01:intron | 20.0% |
| ! | ATTGAATACTTACAATGTGT+AGG | + | Chr2:50227866-50227885 | None:intergenic | 25.0% |
| ! | TAATATTACTAAGTCTTGGA+TGG | - | Chr2:50227752-50227771 | MsG0280009317.01.T01:CDS | 25.0% |
| AATCTACACCATACTTACTT+TGG | + | Chr2:50227580-50227599 | None:intergenic | 30.0% | |
| ATCTACACCATACTTACTTT+GGG | + | Chr2:50227579-50227598 | None:intergenic | 30.0% | |
| CTGCTAATATTACTAAGTCT+TGG | - | Chr2:50227748-50227767 | MsG0280009317.01.T01:CDS | 30.0% | |
| CTTTCTTGGCTACAAATGAA+TGG | + | Chr2:50227670-50227689 | None:intergenic | 35.0% | |
| GCAATAGAAAGAAGGCTAAA+TGG | - | Chr2:50227547-50227566 | MsG0280009317.01.T01:CDS | 35.0% | |
| TATCCACAATCATTGCCATT+AGG | + | Chr2:50227696-50227715 | None:intergenic | 35.0% | |
| TATTACTAAGTCTTGGATGG+AGG | - | Chr2:50227755-50227774 | MsG0280009317.01.T01:CDS | 35.0% | |
| TCATCTTCAGTTAATGTCGT+TGG | + | Chr2:50227892-50227911 | None:intergenic | 35.0% | |
| ! | GTATGATTTGCGAAACCAAT+GGG | - | Chr2:50227915-50227934 | MsG0280009317.01.T01:CDS | 35.0% |
| ! | TGTATGATTTGCGAAACCAA+TGG | - | Chr2:50227914-50227933 | MsG0280009317.01.T01:CDS | 35.0% |
| CAACCTAATGGCAATGATTG+TGG | - | Chr2:50227690-50227709 | MsG0280009317.01.T01:intron | 40.0% | |
| CAAGAAAACAAGTTGCCCAT+TGG | + | Chr2:50227933-50227952 | None:intergenic | 40.0% | |
| GTTTCGTCCCAAAGTAAGTA+TGG | - | Chr2:50227569-50227588 | MsG0280009317.01.T01:CDS | 40.0% | |
| TAGCCAAGAAAGCAACCTAA+TGG | - | Chr2:50227678-50227697 | MsG0280009317.01.T01:intron | 40.0% | |
| ! | TTGCCATTAGGTTGCTTTCT+TGG | + | Chr2:50227684-50227703 | None:intergenic | 40.0% |
| TGCTCAGGGCAATAGAAAGA+AGG | - | Chr2:50227539-50227558 | MsG0280009317.01.T01:CDS | 45.0% | |
| ! | AGAGGTTCATCAACTTGCTC+AGG | - | Chr2:50227524-50227543 | MsG0280009317.01.T01:CDS | 45.0% |
| ! | GAGGTTCATCAACTTGCTCA+GGG | - | Chr2:50227525-50227544 | MsG0280009317.01.T01:CDS | 45.0% |
| !! | ATAAGTAATTAATATTAATA+AGG | + | Chr2:50227838-50227857 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 50227516 | 50227969 | 50227516 | ID=MsG0280009317.01;Name=MsG0280009317.01 |
| Chr2 | mRNA | 50227516 | 50227969 | 50227516 | ID=MsG0280009317.01.T01;Parent=MsG0280009317.01;Name=MsG0280009317.01.T01;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|0|0|3|0|84 |
| Chr2 | exon | 50227516 | 50227608 | 50227516 | ID=MsG0280009317.01.T01:exon:8202;Parent=MsG0280009317.01.T01 |
| Chr2 | exon | 50227709 | 50227804 | 50227709 | ID=MsG0280009317.01.T01:exon:8201;Parent=MsG0280009317.01.T01 |
| Chr2 | exon | 50227904 | 50227969 | 50227904 | ID=MsG0280009317.01.T01:exon:8200;Parent=MsG0280009317.01.T01 |
| Chr2 | CDS | 50227904 | 50227969 | 50227904 | ID=MsG0280009317.01.T01:cds;Parent=MsG0280009317.01.T01 |
| Chr2 | CDS | 50227709 | 50227804 | 50227709 | ID=MsG0280009317.01.T01:cds;Parent=MsG0280009317.01.T01 |
| Chr2 | CDS | 50227516 | 50227608 | 50227516 | ID=MsG0280009317.01.T01:cds;Parent=MsG0280009317.01.T01 |
| Gene Sequence |
| Protein sequence |
