AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009646.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009646.01.T01 MTR_1g014250 88.372 129 15 0 19 147 198 326 1.49e-72 223
MsG0280009646.01.T01 MTR_1g014250 88.372 129 15 0 19 147 321 449 8.52e-71 222
MsG0280009646.01.T01 MTR_5g007060 73.469 98 11 1 19 116 185 267 1.49e-36 127
MsG0280009646.01.T01 MTR_1g014220 37.500 136 44 1 21 156 318 412 4.13e-18 80.9
MsG0280009646.01.T01 MTR_3g051190 34.959 123 80 0 21 143 349 471 9.91e-15 71.2
MsG0280009646.01.T01 MTR_1g103540 43.478 69 37 2 78 145 18 85 1.31e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009646.01.T01 AT5G25050 65.354 127 44 0 21 147 318 444 1.17e-50 169
MsG0280009646.01.T01 AT5G25040 60.630 127 50 0 21 147 169 295 6.22e-47 156
MsG0280009646.01.T01 AT5G25040 60.630 127 50 0 21 147 316 442 1.34e-45 156
MsG0280009646.01.T01 AT5G25040 60.630 127 50 0 21 147 341 467 1.55e-45 156
MsG0280009646.01.T01 AT1G79710 59.055 127 52 0 21 147 322 448 5.96e-44 152
MsG0280009646.01.T01 AT1G79710 59.055 127 52 0 21 147 322 448 5.96e-44 152
MsG0280009646.01.T01 AT5G10820 54.610 141 62 1 16 156 328 466 6.57e-41 144
MsG0280009646.01.T01 AT5G25040 61.818 110 42 0 21 130 316 425 7.16e-37 132
MsG0280009646.01.T01 AT1G79710 53.247 77 36 0 21 97 322 398 4.32e-20 86.3
MsG0280009646.01.T01 AT5G25040 56.250 64 28 0 21 84 316 379 5.11e-16 74.7
MsG0280009646.01.T01 AT5G54860 31.250 128 88 0 16 143 339 466 1.17e-14 71.2
MsG0280009646.01.T01 AT1G04570 34.884 129 82 2 19 146 356 483 4.12e-13 66.6
MsG0280009646.01.T01 AT1G04570 34.884 129 82 2 19 146 208 335 5.07e-13 66.2
MsG0280009646.01.T01 AT2G32040 34.921 126 80 2 21 145 272 396 9.41e-13 65.5
MsG0280009646.01.T01 AT2G32040 34.921 126 80 2 21 145 403 527 1.17e-12 65.5
MsG0280009646.01.T01 AT2G33280 33.333 129 84 2 19 146 175 302 4.75e-12 63.5
MsG0280009646.01.T01 AT2G33280 33.333 129 84 2 19 146 249 376 5.56e-12 63.2
MsG0280009646.01.T01 AT2G33280 33.333 129 84 2 19 146 357 484 6.31e-12 63.2

Find 22 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAAAGTTTGAGAATCTT+TGG 0.246673 2:-55761420 MsG0280009646.01.T01:CDS
TGGTATGTAATGCGCCTAAA+AGG 0.258930 2:+55761769 None:intergenic
CAGTTGCTCTATGGTCTATC+AGG 0.276942 2:-55761700 MsG0280009646.01.T01:CDS
CTGGAATTCTTTCATCATCA+TGG 0.371519 2:-55761480 MsG0280009646.01.T01:CDS
CTTTATCCATGTGGCATTGA+AGG 0.384331 2:-55761538 MsG0280009646.01.T01:CDS
ATGAGATTGAACCTGAAGTT+TGG 0.425035 2:-55761655 MsG0280009646.01.T01:CDS
TTTCTTCCACAGGAGAGTAT+AGG 0.435044 2:-55761822 None:intergenic
CTTTCATCATCATGGGGTGT+TGG 0.456678 2:-55761472 MsG0280009646.01.T01:CDS
ATCACAACAAAGACGTAATC+CGG 0.462520 2:+55761628 None:intergenic
AATCCGGTATACCAAACTTC+AGG 0.469780 2:+55761644 None:intergenic
TGGAATTCTTTCATCATCAT+GGG 0.479682 2:-55761479 MsG0280009646.01.T01:CDS
GAACCTGAAGTTTGGTATAC+CGG 0.479911 2:-55761647 MsG0280009646.01.T01:CDS
GGTATGTAATGCGCCTAAAA+GGG 0.490485 2:+55761770 None:intergenic
TACATACCAACACGTGCTAA+AGG 0.519509 2:-55761755 MsG0280009646.01.T01:CDS
ACAAGCATAGGCATCCACTT+TGG 0.558532 2:+55761574 None:intergenic
CGTAATCCTTTAGCACGTGT+TGG 0.560104 2:+55761749 None:intergenic
TTAATGTCGATTGATAATGC+TGG 0.562494 2:-55761499 MsG0280009646.01.T01:CDS
GGAATTCTTTCATCATCATG+GGG 0.599338 2:-55761478 MsG0280009646.01.T01:CDS
TTTGAGCTGAGTACAAGCAT+AGG 0.628993 2:+55761562 None:intergenic
AGCTCAAAGCTTTATCCATG+TGG 0.635750 2:-55761547 MsG0280009646.01.T01:CDS
AGTTGCTCTATGGTCTATCA+GGG 0.638082 2:-55761699 MsG0280009646.01.T01:CDS
AATGTTCCTTCAATGCCACA+TGG 0.644480 2:+55761532 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTATATTTTAAATGTGGTT+TGG - Chr2:55761773-55761792 MsG0280009646.01.T01:CDS 20.0%
!!! ATGACAGTATATTTTAAATG+TGG - Chr2:55761768-55761787 MsG0280009646.01.T01:CDS 20.0%
!! TAAGGTTGATTACCAAAAAA+GGG + Chr2:55761813-55761832 None:intergenic 25.0%
!! TTAAGGTTGATTACCAAAAA+AGG + Chr2:55761814-55761833 None:intergenic 25.0%
!!! TTTGGTTAGTGATTTTGATT+TGG - Chr2:55761724-55761743 MsG0280009646.01.T01:CDS 25.0%
!!! TTTTTTGGTAATCAACCTTA+AGG - Chr2:55761813-55761832 MsG0280009646.01.T01:CDS 25.0%
GAACAAAGTTTGAGAATCTT+TGG - Chr2:55761706-55761725 MsG0280009646.01.T01:CDS 30.0%
TGGAATTCTTTCATCATCAT+GGG - Chr2:55761647-55761666 MsG0280009646.01.T01:CDS 30.0%
! ATATTTTCAATCAGCTCAGT+CGG - Chr2:55761331-55761350 MsG0280009646.01.T01:CDS 30.0%
!! TTAATGTCGATTGATAATGC+TGG - Chr2:55761627-55761646 MsG0280009646.01.T01:CDS 30.0%
!!! ATAGTAATCCAACCCTTTTT+TGG - Chr2:55761798-55761817 MsG0280009646.01.T01:CDS 30.0%
ATCACAACAAAGACGTAATC+CGG + Chr2:55761501-55761520 None:intergenic 35.0%
ATGAGATTGAACCTGAAGTT+TGG - Chr2:55761471-55761490 MsG0280009646.01.T01:CDS 35.0%
CTGGAATTCTTTCATCATCA+TGG - Chr2:55761646-55761665 MsG0280009646.01.T01:CDS 35.0%
GGAATTCTTTCATCATCATG+GGG - Chr2:55761648-55761667 MsG0280009646.01.T01:CDS 35.0%
GTTGATTACCAAAAAAGGGT+TGG + Chr2:55761809-55761828 None:intergenic 35.0%
! ATATGACTTTTAGGCCAAAG+TGG - Chr2:55761538-55761557 MsG0280009646.01.T01:CDS 35.0%
! GCATAGCTCATATGACTTTT+AGG - Chr2:55761529-55761548 MsG0280009646.01.T01:CDS 35.0%
AATCCGGTATACCAAACTTC+AGG + Chr2:55761485-55761504 None:intergenic 40.0%
AATGTTCCTTCAATGCCACA+TGG + Chr2:55761597-55761616 None:intergenic 40.0%
AGTTGCTCTATGGTCTATCA+GGG - Chr2:55761427-55761446 MsG0280009646.01.T01:CDS 40.0%
GAACCTGAAGTTTGGTATAC+CGG - Chr2:55761479-55761498 MsG0280009646.01.T01:CDS 40.0%
GGTATGTAATGCGCCTAAAA+GGG + Chr2:55761359-55761378 None:intergenic 40.0%
TACATACCAACACGTGCTAA+AGG - Chr2:55761371-55761390 MsG0280009646.01.T01:CDS 40.0%
TGGTATGTAATGCGCCTAAA+AGG + Chr2:55761360-55761379 None:intergenic 40.0%
TTTGAGCTGAGTACAAGCAT+AGG + Chr2:55761567-55761586 None:intergenic 40.0%
! AGCTCAAAGCTTTATCCATG+TGG - Chr2:55761579-55761598 MsG0280009646.01.T01:CDS 40.0%
! CTTTATCCATGTGGCATTGA+AGG - Chr2:55761588-55761607 MsG0280009646.01.T01:CDS 40.0%
! TTTTCGACTCAGTTGCTCTA+TGG - Chr2:55761417-55761436 MsG0280009646.01.T01:CDS 40.0%
ACAAGCATAGGCATCCACTT+TGG + Chr2:55761555-55761574 None:intergenic 45.0%
CAGTTGCTCTATGGTCTATC+AGG - Chr2:55761426-55761445 MsG0280009646.01.T01:CDS 45.0%
CGTAATCCTTTAGCACGTGT+TGG + Chr2:55761380-55761399 None:intergenic 45.0%
CTTTCATCATCATGGGGTGT+TGG - Chr2:55761654-55761673 MsG0280009646.01.T01:CDS 45.0%
!! AGCTCAGTCGGTTCCCTTTT+AGG - Chr2:55761343-55761362 MsG0280009646.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 55761315 55761833 55761315 ID=MsG0280009646.01;Name=MsG0280009646.01
Chr2 mRNA 55761315 55761833 55761315 ID=MsG0280009646.01.T01;Parent=MsG0280009646.01;Name=MsG0280009646.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|172
Chr2 exon 55761315 55761833 55761315 ID=MsG0280009646.01.T01:exon:5904;Parent=MsG0280009646.01.T01
Chr2 CDS 55761315 55761833 55761315 ID=MsG0280009646.01.T01:cds;Parent=MsG0280009646.01.T01
Gene Sequence

>MsG0280009646.01.T01

GGAGAGTATAGGTTTCATATTTTCAATCAGCTCAGTCGGTTCCCTTTTAGGCGCATTACATACCAACACGTGCTAAAGGATTACGCATTCAAAGACTTACTCTTTTCGACTCAGTTGCTCTATGGTCTATCAGGGATGTTTGATCTAATTTTAGTCATGAGATTGAACCTGAAGTTTGGTATACCGGATTACGTCTTTGTTGTGATTGTCGAAAGCATAGCTCATATGACTTTTAGGCCAAAGTGGATGCCTATGCTTGTACTCAGCTCAAAGCTTTATCCATGTGGCATTGAAGGAACATTCTTTGCTCTATTAATGTCGATTGATAATGCTGGAATTCTTTCATCATCATGGGGTGTTGGATTTGTACTTCATGTACTAAAGATTACAAGAACAAAGTTTGAGAATCTTTGGTTAGTGATTTTGATTTGGAATATTTTATTTCGTATTTTAATGACAGTATATTTTAAATGTGGTTTGGATATAGTAATCCAACCCTTTTTTGGTAATCAACCTTAA

Protein sequence

>MsG0280009646.01.T01

GEYRFHIFNQLSRFPFRRITYQHVLKDYAFKDLLFSTQLLYGLSGMFDLILVMRLNLKFGIPDYVFVVIVESIAHMTFRPKWMPMLVLSSKLYPCGIEGTFFALLMSIDNAGILSSSWGVGFVLHVLKITRTKFENLWLVILIWNILFRILMTVYFKCGLDIVIQPFFGNQP*