Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027766.01.T01 | XP_004506453.1 | 78.689 | 61 | 12 | 1 | 17 | 76 | 380 | 440 | 8.59E-19 | 90.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027766.01.T01 | A0A1S2YL64 | 78.689 | 61 | 12 | 1 | 17 | 76 | 380 | 440 | 4.10e-19 | 90.1 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0580027766.01 | 0.828584 | 8.288608e-55 | 8.273511e-52 |
| MsG0080048618.01 | MsG0580027766.01 | 0.804475 | 2.122484e-49 | 1.095372e-46 |
| MsG0080048874.01 | MsG0580027766.01 | 0.826955 | 2.041605e-54 | 1.943416e-51 |
| MsG0180000240.01 | MsG0580027766.01 | -0.803860 | 2.849464e-49 | 1.447571e-46 |
| MsG0180000470.01 | MsG0580027766.01 | 0.848025 | 7.968417e-60 | 1.442799e-56 |
| MsG0180000546.01 | MsG0580027766.01 | 0.818905 | 1.535504e-52 | 1.164414e-49 |
| MsG0180000550.01 | MsG0580027766.01 | 0.839413 | 1.608254e-57 | 2.219389e-54 |
| MsG0180000650.01 | MsG0580027766.01 | 0.814794 | 1.285238e-51 | 8.706900e-49 |
| MsG0180001063.01 | MsG0580027766.01 | 0.835437 | 1.679194e-56 | 2.053310e-53 |
| MsG0180001141.01 | MsG0580027766.01 | 0.816547 | 5.228286e-52 | 3.715577e-49 |
| MsG0180001342.01 | MsG0580027766.01 | 0.824990 | 5.981199e-54 | 5.383035e-51 |
| MsG0180001663.01 | MsG0580027766.01 | 0.806534 | 7.850515e-50 | 4.271521e-47 |
| MsG0180002008.01 | MsG0580027766.01 | 0.800971 | 1.122226e-48 | 5.296734e-46 |
| MsG0180002089.01 | MsG0580027766.01 | 0.801652 | 8.139082e-49 | 3.907878e-46 |
| MsG0180002282.01 | MsG0580027766.01 | 0.849681 | 2.766926e-60 | 5.287200e-57 |
| MsG0180002482.01 | MsG0580027766.01 | 0.801950 | 7.070993e-49 | 3.420817e-46 |
| MsG0180002488.01 | MsG0580027766.01 | 0.803345 | 3.643949e-49 | 1.826726e-46 |
| MsG0180002842.01 | MsG0580027766.01 | 0.838301 | 3.119410e-57 | 4.160279e-54 |
| MsG0180003669.01 | MsG0580027766.01 | 0.834889 | 2.308834e-56 | 2.777076e-53 |
| MsG0180003673.01 | MsG0580027766.01 | 0.806909 | 6.541014e-50 | 3.593537e-47 |
| MsG0180003686.01 | MsG0580027766.01 | 0.813038 | 3.132702e-51 | 2.023659e-48 |
| MsG0180003952.01 | MsG0580027766.01 | 0.814666 | 1.371901e-51 | 9.261717e-49 |
| MsG0180004264.01 | MsG0580027766.01 | 0.823880 | 1.091495e-53 | 9.516780e-51 |
| MsG0180004926.01 | MsG0580027766.01 | 0.805783 | 1.129645e-49 | 6.029907e-47 |
| MsG0180005050.01 | MsG0580027766.01 | 0.818997 | 1.463354e-52 | 1.112553e-49 |
| MsG0180005960.01 | MsG0580027766.01 | 0.806332 | 8.658111e-50 | 4.686752e-47 |
| MsG0180006220.01 | MsG0580027766.01 | 0.805453 | 1.325040e-49 | 7.013061e-47 |
| MsG0180006257.01 | MsG0580027766.01 | 0.806228 | 9.108529e-50 | 4.917726e-47 |
| MsG0280006324.01 | MsG0580027766.01 | 0.800547 | 1.369540e-48 | 6.394458e-46 |
| MsG0280010722.01 | MsG0580027766.01 | 0.829570 | 4.781501e-55 | 4.912433e-52 |
| MsG0280011032.01 | MsG0580027766.01 | 0.819584 | 1.075432e-52 | 8.310028e-50 |
| MsG0280011156.01 | MsG0580027766.01 | 0.804700 | 1.904758e-49 | 9.887595e-47 |
| MsG0280011175.01 | MsG0580027766.01 | 0.820784 | 5.710584e-53 | 4.562081e-50 |
| MsG0280011376.01 | MsG0580027766.01 | 0.818653 | 1.751575e-52 | 1.318863e-49 |
| MsG0380011609.01 | MsG0580027766.01 | 0.833842 | 4.226257e-56 | 4.927017e-53 |
| MsG0380011895.01 | MsG0580027766.01 | 0.801889 | 7.277008e-49 | 3.515338e-46 |
| MsG0380012330.01 | MsG0580027766.01 | 0.804423 | 2.175316e-49 | 1.121130e-46 |
| MsG0380014173.01 | MsG0580027766.01 | 0.821116 | 4.790951e-53 | 3.863075e-50 |
| MsG0380014251.01 | MsG0580027766.01 | 0.802377 | 5.775761e-49 | 2.824996e-46 |
| MsG0380014770.01 | MsG0580027766.01 | 0.842774 | 2.106193e-58 | 3.226889e-55 |
| MsG0480018659.01 | MsG0580027766.01 | 0.810650 | 1.037181e-50 | 6.287567e-48 |
| MsG0480018660.01 | MsG0580027766.01 | 0.812604 | 3.900151e-51 | 2.490597e-48 |
| MsG0480018883.01 | MsG0580027766.01 | 0.831709 | 1.431076e-55 | 1.565722e-52 |
| MsG0480018964.01 | MsG0580027766.01 | 0.805763 | 1.141125e-49 | 6.088053e-47 |
| MsG0480021164.01 | MsG0580027766.01 | 0.806736 | 7.118381e-50 | 3.893266e-47 |
| MsG0480021274.01 | MsG0580027766.01 | 0.837607 | 4.703707e-57 | 6.142271e-54 |
| MsG0480021536.01 | MsG0580027766.01 | 0.828256 | 9.944664e-55 | 9.832023e-52 |
| MsG0480021558.01 | MsG0580027766.01 | 0.821684 | 3.543044e-53 | 2.902481e-50 |
| MsG0480021853.01 | MsG0580027766.01 | 0.830634 | 2.629480e-55 | 2.786317e-52 |
| MsG0480021925.01 | MsG0580027766.01 | 0.828487 | 8.750395e-55 | 8.710107e-52 |
| MsG0480022068.01 | MsG0580027766.01 | 0.820599 | 6.299109e-53 | 5.006029e-50 |
| MsG0480022213.01 | MsG0580027766.01 | -0.823771 | 1.157651e-53 | 1.006332e-50 |
| MsG0480022222.01 | MsG0580027766.01 | 0.810627 | 1.049117e-50 | 6.356045e-48 |
| MsG0480022259.01 | MsG0580027766.01 | 0.861464 | 1.007358e-63 | 2.853099e-60 |
| MsG0480022262.01 | MsG0580027766.01 | 0.850740 | 1.396371e-60 | 2.762362e-57 |
| MsG0480022951.01 | MsG0580027766.01 | 0.814408 | 1.564023e-51 | 1.048668e-48 |
| MsG0480023251.01 | MsG0580027766.01 | 0.803418 | 3.519573e-49 | 1.767760e-46 |
| MsG0480023654.01 | MsG0580027766.01 | 0.812587 | 3.933207e-51 | 2.510669e-48 |
| MsG0480023707.01 | MsG0580027766.01 | 0.814279 | 1.670515e-51 | 1.116097e-48 |
| MsG0580024363.01 | MsG0580027766.01 | -0.815181 | 1.054550e-51 | 7.219011e-49 |
| MsG0580024552.01 | MsG0580027766.01 | 0.842759 | 2.125766e-58 | 3.255240e-55 |
| MsG0580025135.01 | MsG0580027766.01 | 0.815513 | 8.894113e-52 | 6.144992e-49 |
| MsG0580025201.01 | MsG0580027766.01 | 0.802605 | 5.181322e-49 | 2.548899e-46 |
| MsG0580025448.01 | MsG0580027766.01 | 0.828532 | 8.530960e-55 | 8.502912e-52 |
| MsG0580025638.01 | MsG0580027766.01 | 0.855504 | 6.036136e-62 | 1.396538e-58 |
| MsG0380015164.01 | MsG0580027766.01 | 0.805291 | 1.433113e-49 | 7.553257e-47 |
| MsG0380015566.01 | MsG0580027766.01 | 0.826188 | 3.111368e-54 | 2.897248e-51 |
| MsG0380015720.01 | MsG0580027766.01 | 0.802046 | 6.754585e-49 | 3.275857e-46 |
| MsG0380016285.01 | MsG0580027766.01 | 0.816419 | 5.584646e-52 | 3.954989e-49 |
| MsG0380016475.01 | MsG0580027766.01 | 0.811867 | 5.647004e-51 | 3.535371e-48 |
| MsG0380016581.01 | MsG0580027766.01 | 0.800230 | 1.588639e-48 | 7.358156e-46 |
| MsG0380016910.01 | MsG0580027766.01 | 0.827262 | 1.723715e-54 | 1.655691e-51 |
| MsG0380017343.01 | MsG0580027766.01 | 0.813597 | 2.361078e-51 | 1.548614e-48 |
| MsG0380017543.01 | MsG0580027766.01 | 0.822045 | 2.921774e-53 | 2.418156e-50 |
| MsG0380017551.01 | MsG0580027766.01 | -0.809052 | 2.289515e-50 | 1.330439e-47 |
| MsG0380017680.01 | MsG0580027766.01 | 0.841675 | 4.114461e-58 | 6.090104e-55 |
| MsG0480018163.01 | MsG0580027766.01 | 0.814258 | 1.688809e-51 | 1.127648e-48 |
| MsG0480018236.01 | MsG0580027766.01 | 0.824664 | 7.140929e-54 | 6.367349e-51 |
| MsG0480018372.01 | MsG0580027766.01 | 0.858229 | 9.504575e-63 | 2.408660e-59 |
| MsG0480018425.01 | MsG0580027766.01 | 0.809985 | 1.443225e-50 | 8.597395e-48 |
| MsG0580027679.01 | MsG0580027766.01 | 0.807361 | 5.246990e-50 | 2.916636e-47 |
| MsG0580027688.01 | MsG0580027766.01 | 0.806388 | 8.428948e-50 | 4.569023e-47 |
| MsG0580027766.01 | MsG0580027767.01 | 0.893537 | 5.731726e-75 | 5.482743e-71 |
| MsG0580027766.01 | MsG0580027833.01 | 0.806834 | 6.786258e-50 | 3.721045e-47 |
| MsG0580027766.01 | MsG0580027922.01 | 0.816464 | 5.454986e-52 | 3.867920e-49 |
| MsG0580027766.01 | MsG0580028990.01 | 0.831308 | 1.797230e-55 | 1.942737e-52 |
| MsG0580027766.01 | MsG0580029500.01 | -0.813470 | 2.518034e-51 | 1.645907e-48 |
| MsG0580027766.01 | MsG0580029999.01 | -0.812197 | 4.785760e-51 | 3.023241e-48 |
| MsG0580027766.01 | MsG0680030316.01 | 0.804703 | 1.902320e-49 | 9.875660e-47 |
| MsG0580027766.01 | MsG0680030880.01 | 0.820961 | 5.200256e-53 | 4.175379e-50 |
| MsG0580027766.01 | MsG0680030882.01 | 0.827703 | 1.350995e-54 | 1.314312e-51 |
| MsG0580027766.01 | MsG0680030886.01 | 0.814400 | 1.570465e-51 | 1.052737e-48 |
| MsG0580027766.01 | MsG0680031355.01 | 0.815938 | 7.152741e-52 | 4.999332e-49 |
| MsG0580027766.01 | MsG0680031379.01 | 0.810729 | 9.973256e-51 | 6.058827e-48 |
| MsG0580027766.01 | MsG0680031646.01 | 0.807704 | 4.437435e-50 | 2.488741e-47 |
| MsG0580027766.01 | MsG0680032157.01 | 0.814551 | 1.454220e-51 | 9.787898e-49 |
| MsG0580027766.01 | MsG0680032158.01 | 0.814265 | 1.682151e-51 | 1.123440e-48 |
| MsG0580027766.01 | MsG0680033412.01 | 0.809973 | 1.452209e-50 | 8.647799e-48 |
| MsG0580027766.01 | MsG0680034464.01 | 0.812569 | 3.968352e-51 | 2.531919e-48 |
| MsG0580027766.01 | MsG0680034565.01 | 0.807766 | 4.304955e-50 | 2.418324e-47 |
| MsG0580027766.01 | MsG0680035694.01 | 0.833661 | 4.690358e-56 | 5.437700e-53 |
| MsG0580027766.01 | MsG0680035828.01 | 0.825278 | 5.114418e-54 | 4.640654e-51 |
| MsG0580027766.01 | MsG0680035909.01 | 0.829750 | 4.322576e-55 | 4.465030e-52 |
| MsG0580027766.01 | MsG0780035948.01 | 0.826969 | 2.025605e-54 | 1.929050e-51 |
| MsG0580027766.01 | MsG0780037054.01 | 0.802011 | 6.868219e-49 | 3.327955e-46 |
| MsG0580027766.01 | MsG0780037592.01 | 0.804899 | 1.730521e-49 | 9.030473e-47 |
| MsG0580027766.01 | MsG0780037965.01 | 0.804947 | 1.691399e-49 | 8.836739e-47 |
| MsG0580027766.01 | MsG0780038439.01 | 0.812635 | 3.838338e-51 | 2.453249e-48 |
| MsG0580027766.01 | MsG0780038906.01 | 0.810352 | 1.203190e-50 | 7.236076e-48 |
| MsG0580027766.01 | MsG0780039144.01 | 0.801884 | 7.294368e-49 | 3.523251e-46 |
| MsG0580027766.01 | MsG0780039631.01 | 0.811933 | 5.464596e-51 | 3.427068e-48 |
| MsG0580027766.01 | MsG0780039867.01 | -0.827851 | 1.245353e-54 | 1.216819e-51 |
| MsG0580027766.01 | MsG0780040576.01 | -0.806283 | 8.866944e-50 | 4.793811e-47 |
| MsG0580027766.01 | MsG0780040596.01 | 0.845519 | 3.860712e-59 | 6.450642e-56 |
| MsG0580027766.01 | MsG0780040704.01 | 0.826960 | 2.036147e-54 | 1.938528e-51 |
| MsG0580027766.01 | MsG0780040788.01 | 0.807778 | 4.280647e-50 | 2.405436e-47 |
| MsG0580027766.01 | MsG0780041456.01 | 0.835185 | 1.944117e-56 | 2.359349e-53 |
| MsG0580027766.01 | MsG0780041507.01 | 0.820921 | 5.313619e-53 | 4.261343e-50 |
| MsG0580027766.01 | MsG0880041836.01 | 0.824303 | 8.683291e-54 | 7.661023e-51 |
| MsG0580027766.01 | MsG0880042161.01 | 0.806805 | 6.882473e-50 | 3.770979e-47 |
| MsG0580027766.01 | MsG0880042232.01 | 0.829915 | 3.940517e-55 | 4.089662e-52 |
| MsG0580027766.01 | MsG0880042294.01 | 0.848686 | 5.233568e-60 | 9.682188e-57 |
| MsG0580027766.01 | MsG0880042301.01 | 0.802637 | 5.105036e-49 | 2.513348e-46 |
| MsG0580027766.01 | MsG0880042314.01 | 0.826146 | 3.183491e-54 | 2.960818e-51 |
| MsG0580027766.01 | MsG0880042404.01 | 0.827074 | 1.912742e-54 | 1.827005e-51 |
| MsG0580027766.01 | MsG0880042698.01 | 0.826256 | 2.997769e-54 | 2.796826e-51 |
| MsG0580027766.01 | MsG0880042966.01 | 0.812392 | 4.338064e-51 | 2.754973e-48 |
| MsG0580027766.01 | MsG0880043454.01 | 0.815638 | 8.343768e-52 | 5.784567e-49 |
| MsG0580027766.01 | MsG0880044416.01 | 0.816918 | 4.316965e-52 | 3.099332e-49 |
| MsG0580027766.01 | MsG0880044455.01 | -0.821649 | 3.609759e-53 | 2.954144e-50 |
| MsG0580027766.01 | MsG0880044574.01 | 0.832132 | 1.125405e-55 | 1.246469e-52 |
| MsG0580027766.01 | MsG0880045111.01 | 0.807635 | 4.591002e-50 | 2.570211e-47 |
| MsG0580027766.01 | MsG0880045371.01 | 0.804666 | 1.936012e-49 | 1.004105e-46 |
| MsG0580027766.01 | MsG0880045555.01 | -0.824987 | 5.993279e-54 | 5.393307e-51 |
| MsG0580027766.01 | MsG0880045736.01 | 0.822160 | 2.748368e-53 | 2.282102e-50 |
| MsG0580027766.01 | MsG0880045896.01 | 0.825348 | 4.923615e-54 | 4.476207e-51 |
| MsG0580027766.01 | MsG0880045937.01 | 0.850350 | 1.797673e-60 | 3.511006e-57 |
| MsG0580027766.01 | MsG0880046425.01 | 0.816967 | 4.208825e-52 | 3.026056e-49 |
| MsG0580027766.01 | MsG0880047144.01 | 0.807995 | 3.849378e-50 | 2.175422e-47 |
| MsG0580027766.01 | MsG0880047294.01 | 0.800784 | 1.224991e-48 | 5.754010e-46 |
| MsG0280007058.01 | MsG0580027766.01 | -0.807123 | 5.893036e-50 | 3.255445e-47 |
| MsG0280007547.01 | MsG0580027766.01 | 0.805687 | 1.183514e-49 | 6.301930e-47 |
| MsG0280007864.01 | MsG0580027766.01 | -0.805102 | 1.569765e-49 | 8.233513e-47 |
| MsG0280008615.01 | MsG0580027766.01 | 0.808056 | 3.735675e-50 | 2.114513e-47 |
| MsG0280008894.01 | MsG0580027766.01 | 0.840384 | 8.983840e-58 | 1.277581e-54 |
| MsG0280009317.01 | MsG0580027766.01 | 0.802714 | 4.921270e-49 | 2.427743e-46 |
| MsG0280009395.01 | MsG0580027766.01 | -0.812091 | 5.046927e-51 | 3.178972e-48 |
| MsG0280010443.01 | MsG0580027766.01 | 0.828187 | 1.033372e-54 | 1.019671e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCGATAATGTTGCTGTTAT+AGG | 0.252556 | 5:-68249259 | MsG0580027766.01.T01:CDS |
| CTTCTGTGTCTCTGTTAAAT+AGG | 0.287511 | 5:-68249285 | MsG0580027766.01.T01:CDS |
| ATCCTGTTGTTGCATGTAAT+AGG | 0.349513 | 5:-68249358 | MsG0580027766.01.T01:CDS |
| ACATGCAACAACAGGATATC+TGG | 0.351520 | 5:+68249364 | None:intergenic |
| TCCTGTTGTTGCATGTAATA+GGG | 0.398078 | 5:-68249357 | MsG0580027766.01.T01:CDS |
| CTTCTTCTACCGTTTGATGA+AGG | 0.424965 | 5:+68249187 | None:intergenic |
| AGTCATTTCGATATTCTTGT+TGG | 0.454214 | 5:+68249133 | None:intergenic |
| TTCTTCTACCGTTTGATGAA+GGG | 0.463767 | 5:+68249188 | None:intergenic |
| TCCCTATTACATGCAACAAC+AGG | 0.466654 | 5:+68249356 | None:intergenic |
| TCAAACGGTAGAAGAAGAAA+TGG | 0.486110 | 5:-68249181 | MsG0580027766.01.T01:CDS |
| CTGTTAGACCCTTCATCAAA+CGG | 0.512392 | 5:-68249196 | MsG0580027766.01.T01:CDS |
| TGCAGCTGTAGAATCAGTCA+CGG | 0.640246 | 5:+68249312 | None:intergenic |
| AGGCTCAGAAATTCAGACAA+CGG | 0.673554 | 5:-68249239 | MsG0580027766.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCCTGTTGTTGCATGTAAT+AGG | - | Chr5:68249150-68249169 | MsG0580027766.01.T01:CDS | 35.0% | |
| CTTCTGTGTCTCTGTTAAAT+AGG | - | Chr5:68249223-68249242 | MsG0580027766.01.T01:CDS | 35.0% | |
| TCAAACGGTAGAAGAAGAAA+TGG | - | Chr5:68249327-68249346 | MsG0580027766.01.T01:CDS | 35.0% | |
| TCCTGTTGTTGCATGTAATA+GGG | - | Chr5:68249151-68249170 | MsG0580027766.01.T01:CDS | 35.0% | |
| TTCTTCTACCGTTTGATGAA+GGG | + | Chr5:68249323-68249342 | None:intergenic | 35.0% | |
| ! | GTCGATAATGTTGCTGTTAT+AGG | - | Chr5:68249249-68249268 | MsG0580027766.01.T01:CDS | 35.0% |
| ACATGCAACAACAGGATATC+TGG | + | Chr5:68249147-68249166 | None:intergenic | 40.0% | |
| AGGCTCAGAAATTCAGACAA+CGG | - | Chr5:68249269-68249288 | MsG0580027766.01.T01:CDS | 40.0% | |
| CTGTTAGACCCTTCATCAAA+CGG | - | Chr5:68249312-68249331 | MsG0580027766.01.T01:CDS | 40.0% | |
| CTTCTTCTACCGTTTGATGA+AGG | + | Chr5:68249324-68249343 | None:intergenic | 40.0% | |
| TCCCTATTACATGCAACAAC+AGG | + | Chr5:68249155-68249174 | None:intergenic | 40.0% | |
| TGCAGCTGTAGAATCAGTCA+CGG | + | Chr5:68249199-68249218 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 68249135 | 68249395 | 68249135 | ID=MsG0580027766.01;Name=MsG0580027766.01 |
| Chr5 | mRNA | 68249135 | 68249395 | 68249135 | ID=MsG0580027766.01.T01;Parent=MsG0580027766.01;Name=MsG0580027766.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|86 |
| Chr5 | exon | 68249135 | 68249395 | 68249135 | ID=MsG0580027766.01.T01:exon:18867;Parent=MsG0580027766.01.T01 |
| Chr5 | CDS | 68249135 | 68249395 | 68249135 | ID=MsG0580027766.01.T01:cds;Parent=MsG0580027766.01.T01 |
| Gene Sequence |
| Protein sequence |
