Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007685.01.T01 | XP_003594495.2 | 97.183 | 355 | 6 | 1 | 1 | 351 | 1 | 355 | 0 | 697 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047877.01 | MsG0280007685.01 | 0.813982 | 1.942572e-51 | 1.287384e-48 |
| MsG0280007685.01 | MsG0280008419.01 | 0.837942 | 3.859695e-57 | 5.092096e-54 |
| MsG0280007685.01 | MsG0380015547.01 | 0.803243 | 3.826461e-49 | 1.913215e-46 |
| MsG0280007685.01 | MsG0380016194.01 | 0.820761 | 5.783152e-53 | 4.617113e-50 |
| MsG0280007685.01 | MsG0480018848.01 | 0.805458 | 1.321818e-49 | 6.996962e-47 |
| MsG0280007685.01 | MsG0480021207.01 | 0.805920 | 1.057318e-49 | 5.663586e-47 |
| MsG0280007685.01 | MsG0480021817.01 | 0.807582 | 4.712019e-50 | 2.634171e-47 |
| MsG0280007685.01 | MsG0480022537.01 | 0.812266 | 4.622614e-51 | 2.925652e-48 |
| MsG0280007685.01 | MsG0580027082.01 | 0.800449 | 1.433590e-48 | 6.677056e-46 |
| MsG0280007685.01 | MsG0580027236.01 | 0.817909 | 2.582102e-52 | 1.904803e-49 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007685.01.T01 | MTR_2g029830 | 97.183 | 355 | 6 | 1 | 1 | 351 | 1 | 355 | 0.0 | 697 |
| MsG0280007685.01.T01 | MTR_2g029820 | 79.272 | 357 | 65 | 3 | 1 | 350 | 1 | 355 | 0.0 | 572 |
| MsG0280007685.01.T01 | MTR_2g029815 | 80.460 | 348 | 63 | 2 | 1 | 343 | 1 | 348 | 0.0 | 569 |
| MsG0280007685.01.T01 | MTR_2g029800 | 80.870 | 345 | 57 | 3 | 1 | 338 | 1 | 343 | 0.0 | 566 |
| MsG0280007685.01.T01 | MTR_2g029830 | 96.809 | 282 | 5 | 1 | 74 | 351 | 6 | 287 | 0.0 | 551 |
| MsG0280007685.01.T01 | MTR_2g028980 | 70.112 | 358 | 97 | 4 | 1 | 351 | 1 | 355 | 1.01e-179 | 502 |
| MsG0280007685.01.T01 | MTR_2g029740 | 70.112 | 358 | 97 | 4 | 1 | 351 | 1 | 355 | 3.39e-178 | 498 |
| MsG0280007685.01.T01 | MTR_2g029750 | 69.553 | 358 | 98 | 4 | 1 | 351 | 1 | 354 | 1.51e-177 | 496 |
| MsG0280007685.01.T01 | MTR_2g029860 | 69.602 | 352 | 95 | 4 | 4 | 349 | 6 | 351 | 1.23e-173 | 486 |
| MsG0280007685.01.T01 | MTR_2g029850 | 70.506 | 356 | 92 | 5 | 1 | 349 | 1 | 350 | 6.70e-173 | 484 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007685.01.T01 | AT5G06730 | 53.602 | 347 | 153 | 2 | 8 | 350 | 13 | 355 | 2.33e-127 | 369 |
| MsG0280007685.01.T01 | AT2G38380 | 55.090 | 334 | 135 | 4 | 23 | 351 | 26 | 349 | 8.43e-126 | 365 |
| MsG0280007685.01.T01 | AT5G06720 | 55.280 | 322 | 140 | 1 | 8 | 325 | 12 | 333 | 2.05e-125 | 363 |
| MsG0280007685.01.T01 | AT2G38390 | 53.453 | 333 | 140 | 4 | 24 | 351 | 27 | 349 | 3.66e-123 | 358 |
| MsG0280007685.01.T01 | AT3G32980 | 53.977 | 352 | 153 | 4 | 1 | 343 | 1 | 352 | 1.11e-122 | 357 |
| MsG0280007685.01.T01 | AT3G49120 | 54.412 | 340 | 146 | 4 | 13 | 343 | 14 | 353 | 6.06e-122 | 355 |
| MsG0280007685.01.T01 | AT3G49110 | 54.070 | 344 | 147 | 5 | 9 | 343 | 13 | 354 | 5.01e-120 | 350 |
| MsG0280007685.01.T01 | AT4G08770 | 54.375 | 320 | 140 | 3 | 24 | 337 | 20 | 339 | 5.73e-119 | 348 |
| MsG0280007685.01.T01 | AT4G08780 | 53.125 | 320 | 144 | 3 | 24 | 337 | 20 | 339 | 4.38e-117 | 343 |
| MsG0280007685.01.T01 | AT5G19880 | 48.936 | 329 | 159 | 4 | 4 | 325 | 3 | 329 | 1.57e-106 | 315 |
Find 75 sgRNAs with CRISPR-Local
Find 132 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCATCCCTTCTTCCTAA+AGG | 0.473767 | 2:-19171410 | None:intergenic |
| AAATTGGTAAGGTTTGTGCC+AGG | 0.593226 | 2:-19171781 | None:intergenic |
| AAGAGCTCATTGCGCACAAT+TGG | 0.433277 | 2:+19171660 | MsG0280007685.01.T01:CDS |
| AAGGCTGCAATGATTAAAAT+GGG | 0.452725 | 2:+19171970 | MsG0280007685.01.T01:CDS |
| AAGGCTGCATTTACTGCTCA+AGG | 0.536802 | 2:+19171497 | MsG0280007685.01.T01:CDS |
| AAGTAAGTTGTGTTAAGAGT+TGG | 0.480928 | 2:-19171724 | None:intergenic |
| AAGTTGTGCATCTGAGGAAA+AGG | 0.427974 | 2:-19169741 | None:intergenic |
| ACACCACATAGAAGTTGCTA+AGG | 0.602167 | 2:-19169687 | None:intergenic |
| ACGAGACTAGCAAGCATGCG+GGG | 0.672753 | 2:-19169845 | None:intergenic |
| ACTCCTTAGCAACTTCTATG+TGG | 0.626841 | 2:+19169684 | MsG0280007685.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGCTGCAATGATTAAAAT+GGG | + | Chr2:19171970-19171989 | MsG0280007685.01.T01:CDS | 30.0% | |
| AAGTAAGTTGTGTTAAGAGT+TGG | - | Chr2:19171727-19171746 | None:intergenic | 30.0% | |
| ACTTGCAAATCAAAATCTTC+CGG | + | Chr2:19171448-19171467 | MsG0280007685.01.T01:CDS | 30.0% | |
| ATAGTAGAATCATTAGAGGA+TGG | + | Chr2:19172093-19172112 | MsG0280007685.01.T01:CDS | 30.0% | |
| ATATATATGTACCAGAACAG+AGG | - | Chr2:19170991-19171010 | None:intergenic | 30.0% | |
| ATCCATAGTAGAATCATTAG+AGG | + | Chr2:19172089-19172108 | MsG0280007685.01.T01:CDS | 30.0% | |
| ATCCTCTAATGATTCTACTA+TGG | - | Chr2:19172094-19172113 | None:intergenic | 30.0% | |
| TAAGGCTGCAATGATTAAAA+TGG | + | Chr2:19171969-19171988 | MsG0280007685.01.T01:CDS | 30.0% | |
| TACCTATGCTGAAATAATAC+AGG | + | Chr2:19171364-19171383 | MsG0280007685.01.T01:intron | 30.0% | |
| TCCAATCTTCAAGTGAAAAA+GGG | + | Chr2:19171844-19171863 | MsG0280007685.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 19169587 | 19172340 | 19169587 | ID=MsG0280007685.01;Name=MsG0280007685.01 |
| Chr2 | mRNA | 19169587 | 19172340 | 19169587 | ID=MsG0280007685.01.T01;Parent=MsG0280007685.01;Name=MsG0280007685.01.T01;_AED=0.49;_eAED=0.49;_QI=93|0.66|0.75|1|1|1|4|206|351 |
| Chr2 | exon | 19169587 | 19169898 | 19169587 | ID=MsG0280007685.01.T01:exon:10127;Parent=MsG0280007685.01.T01 |
| Chr2 | exon | 19170807 | 19170998 | 19170807 | ID=MsG0280007685.01.T01:exon:10128;Parent=MsG0280007685.01.T01 |
| Chr2 | exon | 19171386 | 19171538 | 19171386 | ID=MsG0280007685.01.T01:exon:10129;Parent=MsG0280007685.01.T01 |
| Chr2 | exon | 19171643 | 19172340 | 19171643 | ID=MsG0280007685.01.T01:exon:10130;Parent=MsG0280007685.01.T01 |
| Chr2 | five_prime_UTR | 19169587 | 19169679 | 19169587 | ID=MsG0280007685.01.T01:five_prime_utr;Parent=MsG0280007685.01.T01 |
| Chr2 | CDS | 19169680 | 19169898 | 19169680 | ID=MsG0280007685.01.T01:cds;Parent=MsG0280007685.01.T01 |
| Chr2 | CDS | 19170807 | 19170998 | 19170807 | ID=MsG0280007685.01.T01:cds;Parent=MsG0280007685.01.T01 |
| Chr2 | CDS | 19171386 | 19171538 | 19171386 | ID=MsG0280007685.01.T01:cds;Parent=MsG0280007685.01.T01 |
| Chr2 | CDS | 19171643 | 19172134 | 19171643 | ID=MsG0280007685.01.T01:cds;Parent=MsG0280007685.01.T01 |
| Chr2 | three_prime_UTR | 19172135 | 19172340 | 19172135 | ID=MsG0280007685.01.T01:three_prime_utr;Parent=MsG0280007685.01.T01 |
| Gene Sequence |
>MsG0280007685.01.T01 ATGAACTCCTTAGCAACTTCTATGTGGTGTGTTGTGCTTTTAGTTGTGCTTGGAGGACTACCCTTTTCCTCAGATGCACAACTTAGTCCCACTTTTTACAGCAAAACTTGTCCAACTGTTAGTTCCATTGTTAGCAATGTCTTAACAAATGTTTCTAAGACAGATCCCCGCATGCTTGCTAGTCTCGTCAGGCTTCACTTTCATGACTGTTTTGTTCTGGGATGTGATGCCTCAGTTTTGCTGAACAATACTGCTACAATCGTAAGCGAACAACAAGCTTTTCCAAATAACAACTCTCTAAGAGGTTTGGATGTTGTGAATCAGATCAAAACTGCTGTAGAAAGTGCTTGTCCTAACACAGTTTCTTGTGCTGATATTCTTGCACTTGCTGCTCAAGCATCCTCTGTTCTGGCACAAGGTCCTAGTTGGACGGTTCCTTTAGGAAGAAGGGATGGTTTAACCGCAAACCGAACACTTGCAAATCAAAATCTTCCGGCTCCATTCAATTCCTTGGATCAACTTAAGGCTGCATTTACTGCTCAAGGCCTCAATACTACTGATCTAGTGGCTCATACATTTGGAAGAGCTCATTGCGCACAATTGGTTATTCGATTGTACAACTTCAGCAGTACTGGAAGTCCCGATCCAACTCTTAACACAACTTACTTACAACAACTGCGCACAATATGTCCCAATGGTGGACCTGGCACAAACCTTACCAATTTCGATCCAACGACTCCTGATAAATTTGACAAGAACTACTACTCCAATCTTCAAGTGAAAAAGGGCTTACTCCAAAGTGATCAAGAGTTGTTCTCAACATCTGGTGCAGATACCATTAGCATTGTCAACAAATTCAGCACCGATCAAAACGCTTTCTTTGAGAGCTTTAAGGCTGCAATGATTAAAATGGGCAATATTGGTGTGCTAACAGGGACAAAAGGAGAGATTAGAAAACAATGCAACTTTGTGAACTCAAATTCAGCAGAACTAGATTTAGCCACCATAGCATCCATAGTAGAATCATTAGAGGATGGTATTGCTAGTGTAATATAA |
| Protein sequence |
>MsG0280007685.01.T01 MNSLATSMWCVVLLVVLGGLPFSSDAQLSPTFYSKTCPTVSSIVSNVLTNVSKTDPRMLASLVRLHFHDCFVLGCDASVLLNNTATIVSEQQAFPNNNSLRGLDVVNQIKTAVESACPNTVSCADILALAAQASSVLAQGPSWTVPLGRRDGLTANRTLANQNLPAPFNSLDQLKAAFTAQGLNTTDLVAHTFGRAHCAQLVIRLYNFSSTGSPDPTLNTTYLQQLRTICPNGGPGTNLTNFDPTTPDKFDKNYYSNLQVKKGLLQSDQELFSTSGADTISIVNKFSTDQNAFFESFKAAMIKMGNIGVLTGTKGEIRKQCNFVNSNSAELDLATIASIVESLEDGIASVI* |
