AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009758.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009758.01.T01 MTR_2g070110 89.756 205 13 1 1 197 14 218 1.17e-124 350
MsG0280009758.01.T01 MTR_2g070060 83.495 206 25 1 1 197 14 219 7.08e-124 348
MsG0280009758.01.T01 MTR_2g070070 85.437 206 21 1 1 197 14 219 8.43e-121 341
MsG0280009758.01.T01 MTR_2g070180 63.500 200 64 1 2 192 15 214 2.45e-95 276
MsG0280009758.01.T01 MTR_2g070140 62.069 203 68 1 4 197 1 203 1.01e-94 274
MsG0280009758.01.T01 MTR_2g070150 64.737 190 58 1 2 182 15 204 3.71e-92 268
MsG0280009758.01.T01 MTR_8g056940 63.107 206 67 1 1 197 14 219 5.32e-91 265
MsG0280009758.01.T01 MTR_2g070130 58.537 205 76 1 2 197 15 219 2.40e-89 261
MsG0280009758.01.T01 MTR_4g124130 59.903 207 73 2 1 197 14 220 1.02e-86 254
MsG0280009758.01.T01 MTR_3g467420 57.073 205 79 1 2 197 20 224 1.87e-85 251
MsG0280009758.01.T01 MTR_2g070210 57.971 207 76 2 2 197 15 221 5.63e-85 250
MsG0280009758.01.T01 MTR_2g070200 57.282 206 77 2 2 196 15 220 5.75e-85 250
MsG0280009758.01.T01 MTR_3g467430 58.209 201 75 2 1 192 19 219 1.81e-82 244
MsG0280009758.01.T01 MTR_2g070120 56.021 191 75 1 2 183 15 205 7.01e-76 227
MsG0280009758.01.T01 MTR_5g076900 47.087 206 98 3 2 197 17 221 1.05e-60 189
MsG0280009758.01.T01 MTR_0186s0030 64.062 128 37 1 1 119 14 141 5.03e-52 164
MsG0280009758.01.T01 MTR_5g037380 40.758 211 107 3 2 194 16 226 1.18e-45 150
MsG0280009758.01.T01 MTR_5g040430 37.143 210 113 5 2 195 18 224 7.47e-42 140
MsG0280009758.01.T01 MTR_8g087410 41.026 195 101 4 3 184 18 211 3.54e-41 139
MsG0280009758.01.T01 MTR_1g090060 40.102 197 100 5 2 183 14 207 2.27e-40 137
MsG0280009758.01.T01 MTR_7g065750 38.863 211 107 6 2 192 18 226 7.28e-39 133
MsG0280009758.01.T01 MTR_7g065720 37.245 196 108 5 2 184 18 211 9.68e-39 133
MsG0280009758.01.T01 MTR_1g090070 39.698 199 97 5 2 182 20 213 5.66e-38 131
MsG0280009758.01.T01 MTR_8g087425 37.438 203 115 3 2 193 16 217 7.88e-38 130
MsG0280009758.01.T01 MTR_4g059730 40.513 195 102 5 2 183 15 208 8.70e-38 130
MsG0280009758.01.T01 MTR_8g061950 39.535 215 100 6 2 194 16 222 1.01e-37 130
MsG0280009758.01.T01 MTR_7g065660 38.462 195 106 5 2 184 18 210 2.85e-37 129
MsG0280009758.01.T01 MTR_1g090100 40.704 199 96 5 2 183 13 206 3.59e-37 129
MsG0280009758.01.T01 MTR_4g019780 36.082 194 112 3 2 184 19 211 4.24e-37 128
MsG0280009758.01.T01 MTR_6g080440 37.500 192 108 3 5 185 17 207 4.69e-37 128
MsG0280009758.01.T01 MTR_7g065630 37.436 195 104 5 2 184 36 224 1.85e-36 127
MsG0280009758.01.T01 MTR_3g099757 39.706 204 111 5 2 194 16 218 2.54e-36 126
MsG0280009758.01.T01 MTR_1g090150 40.741 189 99 4 2 178 14 201 4.23e-36 126
MsG0280009758.01.T01 MTR_4g019790 41.270 189 97 5 2 178 18 204 5.62e-36 125
MsG0280009758.01.T01 MTR_7g065740 37.441 211 110 7 2 192 18 226 2.12e-35 124
MsG0280009758.01.T01 MTR_7g065710 36.923 195 105 5 2 184 15 203 4.35e-35 123
MsG0280009758.01.T01 MTR_7g065265 40.212 189 99 5 2 178 18 204 1.17e-34 122
MsG0280009758.01.T01 MTR_1g090090 38.974 195 105 5 2 183 14 207 3.06e-34 121
MsG0280009758.01.T01 MTR_2g072120 88.333 60 7 0 34 93 1 60 3.31e-34 116
MsG0280009758.01.T01 MTR_7g065590 38.095 189 103 4 2 178 18 204 6.33e-34 120
MsG0280009758.01.T01 MTR_7g065640 36.702 188 101 5 2 184 18 192 2.33e-32 116
MsG0280009758.01.T01 MTR_7g065270 37.566 189 104 4 2 178 18 204 5.09e-32 115
MsG0280009758.01.T01 MTR_7g065290 38.095 189 103 4 2 178 18 204 6.65e-32 115
MsG0280009758.01.T01 MTR_1g115195 36.041 197 109 5 2 183 14 208 8.99e-32 115
MsG0280009758.01.T01 MTR_7g065600 37.563 197 102 6 1 178 10 204 6.57e-31 112
MsG0280009758.01.T01 MTR_7g065680 36.410 195 106 5 2 184 18 206 6.87e-31 112
MsG0280009758.01.T01 MTR_7g065230 39.894 188 96 6 2 178 18 199 1.02e-30 112
MsG0280009758.01.T01 MTR_7g065700 36.269 193 105 5 2 182 18 204 2.09e-28 106
MsG0280009758.01.T01 MTR_7g065260 36.548 197 104 6 1 178 17 211 6.39e-27 102
MsG0280009758.01.T01 MTR_4g043640 62.500 40 15 0 128 167 46 85 2.87e-13 63.2
MsG0280009758.01.T01 MTR_5g037390 40.476 84 48 1 15 96 11 94 9.25e-12 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009758.01.T01 AT1G17180 58.738 206 76 1 1 197 14 219 4.34e-89 261
MsG0280009758.01.T01 AT1G78380 61.194 201 69 1 1 192 14 214 3.06e-87 256
MsG0280009758.01.T01 AT1G78370 60.938 192 66 1 1 183 14 205 1.52e-85 251
MsG0280009758.01.T01 AT1G17170 57.767 206 77 2 1 197 14 218 1.34e-84 249
MsG0280009758.01.T01 AT1G78360 59.903 207 73 2 1 197 14 220 1.67e-84 249
MsG0280009758.01.T01 AT1G78340 59.686 191 68 1 2 183 15 205 1.35e-82 244
MsG0280009758.01.T01 AT1G78320 58.454 207 76 2 1 197 14 220 1.94e-82 244
MsG0280009758.01.T01 AT1G17190 55.882 204 81 1 1 195 15 218 9.09e-81 239
MsG0280009758.01.T01 AT1G53680 53.623 207 85 1 2 197 18 224 1.73e-80 239
MsG0280009758.01.T01 AT3G43800 50.239 209 91 4 1 196 15 223 1.80e-69 211
MsG0280009758.01.T01 AT1G78320 50.242 207 66 3 1 197 14 193 2.09e-63 194
MsG0280009758.01.T01 AT1G10360 41.500 200 100 5 1 183 15 214 2.02e-45 150
MsG0280009758.01.T01 AT3G09270 41.294 201 106 4 2 190 17 217 4.16e-43 144
MsG0280009758.01.T01 AT2G29420 40.526 190 101 4 2 180 20 208 1.62e-41 140
MsG0280009758.01.T01 AT1G10370 41.791 201 96 6 2 183 16 214 3.44e-41 139
MsG0280009758.01.T01 AT1G59700 40.099 202 103 4 2 185 17 218 2.19e-40 137
MsG0280009758.01.T01 AT1G27140 41.463 205 101 5 2 187 17 221 3.41e-40 137
MsG0280009758.01.T01 AT1G27130 40.000 200 102 5 2 183 17 216 4.10e-39 134
MsG0280009758.01.T01 AT2G29490 37.561 205 115 4 2 194 18 221 3.92e-38 131
MsG0280009758.01.T01 AT2G29480 38.835 206 108 7 2 194 18 218 6.98e-38 130
MsG0280009758.01.T01 AT1G74590 39.344 183 96 4 2 169 18 200 1.26e-37 130
MsG0280009758.01.T01 AT1G59670 41.414 198 98 5 4 183 19 216 1.75e-37 130
MsG0280009758.01.T01 AT2G29440 40.488 205 101 7 2 190 17 216 6.29e-37 128
MsG0280009758.01.T01 AT2G29450 41.624 197 87 8 2 178 17 205 1.29e-35 125
MsG0280009758.01.T01 AT2G29460 37.379 206 111 7 2 194 18 218 1.81e-35 124
MsG0280009758.01.T01 AT1G69930 38.384 198 106 4 2 183 23 220 3.20e-35 124
MsG0280009758.01.T01 AT1G69920 38.071 197 100 5 2 178 45 239 3.30e-35 124
MsG0280009758.01.T01 AT2G29470 37.019 208 112 8 2 195 18 220 3.94e-34 121
MsG0280009758.01.T01 AT5G62480 33.491 212 116 7 2 193 19 225 5.17e-32 116
MsG0280009758.01.T01 AT5G62480 27.830 212 102 8 2 193 19 199 2.00e-16 74.7
MsG0280009758.01.T01 AT5G62480 27.830 212 101 8 2 193 19 198 2.32e-16 74.3

Find 34 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTAAAACCAGCTGTAAAA+AGG 0.236430 2:-57662687 None:intergenic
GCTTGATTATATTGACAAAA+AGG 0.245841 2:+57662029 MsG0280009758.01.T01:CDS
ACGTTTGGATTTCTTGATAT+TGG 0.276844 2:+57662657 MsG0280009758.01.T01:CDS
CTGGGATCTTCTTGTGAATT+GGG 0.295027 2:-57661879 None:intergenic
ACTGGGATCTTCTTGTGAAT+TGG 0.304684 2:-57661880 None:intergenic
TGCAGTACATAGATGAAGTT+TGG 0.332448 2:+57661947 MsG0280009758.01.T01:CDS
GATACATGGTTTAGCATGTT+TGG 0.342998 2:+57661760 None:intergenic
GCATGTTTGGTATGAGGGTT+AGG 0.343737 2:+57661773 None:intergenic
TTGACCCAAGTCATGAGTTT+AGG 0.351167 2:-57662750 None:intergenic
TTTCCATTGTGGATAAGAAC+TGG 0.366518 2:-57661898 None:intergenic
AACGTAGCACCGCCATAAAA+AGG 0.386993 2:-57662639 None:intergenic
GGTTTAGCATGTTTGGTATG+AGG 0.387485 2:+57661767 None:intergenic
TCATACACCTTCTTCTCATC+AGG 0.397779 2:-57662810 None:intergenic
AGAGAGCTCAAGCTAGATTC+TGG 0.444664 2:+57662007 MsG0280009758.01.T01:CDS
GTTTAGCATGTTTGGTATGA+GGG 0.448271 2:+57661768 None:intergenic
AGGACAATGTAGCTTTCAAA+AGG 0.449548 2:+57662543 MsG0280009758.01.T01:intron
AACTCTTCCTGATGAGAAGA+AGG 0.458010 2:+57662803 MsG0280009758.01.T01:CDS
CTAGCTTGAGCTCTCTCATA+AGG 0.462092 2:-57662000 None:intergenic
TGGCAACTTTAAATTTGAAG+TGG 0.517103 2:+57662722 MsG0280009758.01.T01:CDS
GGAGCATGAGGAAAGAAAGA+AGG 0.533036 2:+57662569 MsG0280009758.01.T01:CDS
GGACAATGTAGCTTTCAAAA+GGG 0.544680 2:+57662544 MsG0280009758.01.T01:intron
AGTGTCCTAAACTCATGACT+TGG 0.544683 2:+57662745 MsG0280009758.01.T01:CDS
CAAGACACTAGAAGAAACCT+TGG 0.557125 2:+57662608 MsG0280009758.01.T01:CDS
TTCCATTGTGGATAAGAACT+GGG 0.563471 2:-57661897 None:intergenic
AAGACACTAGAAGAAACCTT+GGG 0.566763 2:+57662609 MsG0280009758.01.T01:CDS
AGCTCTCTCATAAGGATCAG+AGG 0.588678 2:-57661992 None:intergenic
GACAATGTAGCTTTCAAAAG+GGG 0.605449 2:+57662545 MsG0280009758.01.T01:intron
ATCCCAGTTCTTATCCACAA+TGG 0.607984 2:+57661895 MsG0280009758.01.T01:CDS
TTCAAAAGGGGAGGAGCATG+AGG 0.609682 2:+57662557 MsG0280009758.01.T01:CDS
GTGTCCTAAACTCATGACTT+GGG 0.610120 2:+57662746 MsG0280009758.01.T01:CDS
GCTCTCTCATAAGGATCAGA+GGG 0.618631 2:-57661991 None:intergenic
GTGAGCATGAAGAAAGCACT+TGG 0.630117 2:+57662840 MsG0280009758.01.T01:CDS
GACTTGGGTCAAGAGATGCA+TGG 0.639170 2:+57662761 MsG0280009758.01.T01:CDS
AATGTAGCTTTCAAAAGGGG+AGG 0.641248 2:+57662548 MsG0280009758.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAGGATGAAAATTAT+AGG - Chr2:57662075-57662094 None:intergenic 15.0%
!!! ATAATTTTCATCCTTAATTT+TGG + Chr2:57662075-57662094 MsG0280009758.01.T01:intron 15.0%
!! TTAAGCTGTTTATCAAAATA+AGG + Chr2:57662452-57662471 MsG0280009758.01.T01:intron 20.0%
!!! CTATTATCAATTTTTTACTC+TGG + Chr2:57662147-57662166 MsG0280009758.01.T01:intron 20.0%
!!! GAATTTTGAGATTGATTTTT+AGG + Chr2:57662111-57662130 MsG0280009758.01.T01:intron 20.0%
! CATTTACTTGTGAGATTATT+TGG + Chr2:57662239-57662258 MsG0280009758.01.T01:intron 25.0%
! GCTTGATTATATTGACAAAA+AGG + Chr2:57662029-57662048 MsG0280009758.01.T01:CDS 25.0%
!! ATTTTCATCCTTAATTTTGG+TGG + Chr2:57662078-57662097 MsG0280009758.01.T01:intron 25.0%
!!! TTTTACACTTTTGAGACTTA+TGG + Chr2:57662702-57662721 MsG0280009758.01.T01:CDS 25.0%
!!! TTTTCATCCTTAATTTTGGT+GGG + Chr2:57662079-57662098 MsG0280009758.01.T01:intron 25.0%
ACGTTTGGATTTCTTGATAT+TGG + Chr2:57662657-57662676 MsG0280009758.01.T01:CDS 30.0%
ATAAGCTGAAAGCAACTTAT+CGG - Chr2:57662294-57662313 None:intergenic 30.0%
GATGTTTCCATAAACTATCT+TGG - Chr2:57662330-57662349 None:intergenic 30.0%
TGGCAACTTTAAATTTGAAG+TGG + Chr2:57662722-57662741 MsG0280009758.01.T01:CDS 30.0%
TGTAAAACCAGCTGTAAAAA+GGG - Chr2:57662689-57662708 None:intergenic 30.0%
! CACAAATAGATTTTCCATTG+TGG - Chr2:57661912-57661931 None:intergenic 30.0%
! TTTACTCTGGATTAGAAGAT+TGG + Chr2:57662160-57662179 MsG0280009758.01.T01:intron 30.0%
AACACAACCCACCAAAATTA+AGG - Chr2:57662089-57662108 None:intergenic 35.0%
AAGACACTAGAAGAAACCTT+GGG + Chr2:57662609-57662628 MsG0280009758.01.T01:CDS 35.0%
AAGCTCTCCAAGATAGTTTA+TGG + Chr2:57662320-57662339 MsG0280009758.01.T01:intron 35.0%
AGGACAATGTAGCTTTCAAA+AGG + Chr2:57662543-57662562 MsG0280009758.01.T01:intron 35.0%
GACAATGTAGCTTTCAAAAG+GGG + Chr2:57662545-57662564 MsG0280009758.01.T01:intron 35.0%
GGACAATGTAGCTTTCAAAA+GGG + Chr2:57662544-57662563 MsG0280009758.01.T01:intron 35.0%
GTGTAAAACCAGCTGTAAAA+AGG - Chr2:57662690-57662709 None:intergenic 35.0%
TGCAGTACATAGATGAAGTT+TGG + Chr2:57661947-57661966 MsG0280009758.01.T01:CDS 35.0%
TTACAGTCAAATACGACTAC+CGG + Chr2:57662494-57662513 MsG0280009758.01.T01:intron 35.0%
TTCCATTGTGGATAAGAACT+GGG - Chr2:57661900-57661919 None:intergenic 35.0%
TTTCCATTGTGGATAAGAAC+TGG - Chr2:57661901-57661920 None:intergenic 35.0%
AACTCTTCCTGATGAGAAGA+AGG + Chr2:57662803-57662822 MsG0280009758.01.T01:CDS 40.0%
AATGTAGCTTTCAAAAGGGG+AGG + Chr2:57662548-57662567 MsG0280009758.01.T01:intron 40.0%
ACTGGGATCTTCTTGTGAAT+TGG - Chr2:57661883-57661902 None:intergenic 40.0%
AGTGTCCTAAACTCATGACT+TGG + Chr2:57662745-57662764 MsG0280009758.01.T01:CDS 40.0%
ATAAAAAGGCTTGTCACCCA+AGG - Chr2:57662628-57662647 None:intergenic 40.0%
ATCCCAGTTCTTATCCACAA+TGG + Chr2:57661895-57661914 MsG0280009758.01.T01:CDS 40.0%
CAAGACACTAGAAGAAACCT+TGG + Chr2:57662608-57662627 MsG0280009758.01.T01:CDS 40.0%
CTGGGATCTTCTTGTGAATT+GGG - Chr2:57661882-57661901 None:intergenic 40.0%
GAATGTAGGTGTATGCTACT+TGG + Chr2:57662520-57662539 MsG0280009758.01.T01:intron 40.0%
GTGTCCTAAACTCATGACTT+GGG + Chr2:57662746-57662765 MsG0280009758.01.T01:CDS 40.0%
TCATACACCTTCTTCTCATC+AGG - Chr2:57662813-57662832 None:intergenic 40.0%
TTGACCCAAGTCATGAGTTT+AGG - Chr2:57662753-57662772 None:intergenic 40.0%
! CGGCATCTCATAAGTTGTTT+TGG - Chr2:57662274-57662293 None:intergenic 40.0%
! GTTTGATCCCTTTTTACAGC+TGG + Chr2:57662679-57662698 MsG0280009758.01.T01:CDS 40.0%
! TTGGGTGACAAGCCTTTTTA+TGG + Chr2:57662627-57662646 MsG0280009758.01.T01:CDS 40.0%
AACGTAGCACCGCCATAAAA+AGG - Chr2:57662642-57662661 None:intergenic 45.0%
AGAGAGCTCAAGCTAGATTC+TGG + Chr2:57662007-57662026 MsG0280009758.01.T01:CDS 45.0%
AGCTCTCTCATAAGGATCAG+AGG - Chr2:57661995-57662014 None:intergenic 45.0%
CTAGCTTGAGCTCTCTCATA+AGG - Chr2:57662003-57662022 None:intergenic 45.0%
GCATACACCTACATTCAAGC+CGG - Chr2:57662516-57662535 None:intergenic 45.0%
GCTCTCTCATAAGGATCAGA+GGG - Chr2:57661994-57662013 None:intergenic 45.0%
GGAGCATGAGGAAAGAAAGA+AGG + Chr2:57662569-57662588 MsG0280009758.01.T01:CDS 45.0%
! GGTGACAAGCCTTTTTATGG+CGG + Chr2:57662630-57662649 MsG0280009758.01.T01:CDS 45.0%
! TGTAGGTGTATGCTACTTGG+AGG + Chr2:57662523-57662542 MsG0280009758.01.T01:intron 45.0%
!! GTGAGCATGAAGAAAGCACT+TGG + Chr2:57662840-57662859 MsG0280009758.01.T01:CDS 45.0%
!!! TTTTATGGCGGTGCTACGTT+TGG + Chr2:57662642-57662661 MsG0280009758.01.T01:CDS 45.0%
GACTTGGGTCAAGAGATGCA+TGG + Chr2:57662761-57662780 MsG0280009758.01.T01:CDS 50.0%
! TTCAAAAGGGGAGGAGCATG+AGG + Chr2:57662557-57662576 MsG0280009758.01.T01:CDS 50.0%
!! ACGACTACCGGCTTGAATGT+AGG + Chr2:57662506-57662525 MsG0280009758.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 57661775 57662872 57661775 ID=MsG0280009758.01;Name=MsG0280009758.01
Chr2 mRNA 57661775 57662872 57661775 ID=MsG0280009758.01.T01;Parent=MsG0280009758.01;Name=MsG0280009758.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|197
Chr2 exon 57661775 57662050 57661775 ID=MsG0280009758.01.T01:exon:6111;Parent=MsG0280009758.01.T01
Chr2 exon 57662555 57662872 57662555 ID=MsG0280009758.01.T01:exon:6112;Parent=MsG0280009758.01.T01
Chr2 CDS 57661775 57662050 57661775 ID=MsG0280009758.01.T01:cds;Parent=MsG0280009758.01.T01
Chr2 CDS 57662555 57662872 57662555 ID=MsG0280009758.01.T01:cds;Parent=MsG0280009758.01.T01
Gene Sequence

>MsG0280009758.01.T01

ATGTTTGGTATGAGGGTTAGGATTGCATTAGCTGAAAAAAGTGTTAAATATGAGTATAAAGAAGAAAATCTAAGAAATAAGAGTTCTTTACTTCTTCAGATGAACCCAATTCACAAGAAGATCCCAGTTCTTATCCACAATGGAAAATCTATTTGTGAATCTGCAATCATTGTGCAGTACATAGATGAAGTTTGGAATGATAAAGCTTCATTCATGCCCTCTGATCCTTATGAGAGAGCTCAAGCTAGATTCTGGCTTGATTATATTGACAAAAAGCTTTCAAAAGGGGAGGAGCATGAGGAAAGAAAGAAGGAGTTGATCTCTATCTTCAAGACACTAGAAGAAACCTTGGGTGACAAGCCTTTTTATGGCGGTGCTACGTTTGGATTTCTTGATATTGGTTTGATCCCTTTTTACAGCTGGTTTTACACTTTTGAGACTTATGGCAACTTTAAATTTGAAGTGGAGTGTCCTAAACTCATGACTTGGGTCAAGAGATGCATGGAGAAAGAGAGTGTTTCAAAAACTCTTCCTGATGAGAAGAAGGTGTATGATAATGTTGTGAGCATGAAGAAAGCACTTGGCTTTGATTAA

Protein sequence

>MsG0280009758.01.T01

MFGMRVRIALAEKSVKYEYKEENLRNKSSLLLQMNPIHKKIPVLIHNGKSICESAIIVQYIDEVWNDKASFMPSDPYERAQARFWLDYIDKKLSKGEEHEERKKELISIFKTLEETLGDKPFYGGATFGFLDIGLIPFYSWFYTFETYGNFKFEVECPKLMTWVKRCMEKESVSKTLPDEKKVYDNVVSMKKALGFD*