AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004881.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004881.01.T01 MTR_1g088850 97.727 220 5 0 1 220 1 220 1.70e-164 452
MsG0180004881.01.T01 MTR_3g450790 48.571 210 105 3 1 209 1 208 2.65e-69 211
MsG0180004881.01.T01 MTR_3g450930 45.714 210 111 3 1 209 1 208 8.54e-63 195
MsG0180004881.01.T01 MTR_5g090920 44.076 211 114 4 1 209 1 209 1.29e-56 179
MsG0180004881.01.T01 MTR_1g088845 40.865 208 122 1 3 210 4 210 1.48e-56 179
MsG0180004881.01.T01 MTR_3g064700 43.062 209 117 2 1 208 1 208 3.73e-56 178
MsG0180004881.01.T01 MTR_1g088840 39.423 208 125 1 3 210 4 210 1.88e-55 176
MsG0180004881.01.T01 MTR_1g088825 39.423 208 125 1 3 210 4 210 1.88e-55 176
MsG0180004881.01.T01 MTR_5g090910 42.453 212 116 5 1 209 1 209 2.33e-54 173
MsG0180004881.01.T01 MTR_1g026140 37.736 212 127 1 3 209 5 216 5.31e-51 165
MsG0180004881.01.T01 MTR_1g088840 39.506 162 97 1 49 210 3 163 5.68e-41 137
MsG0180004881.01.T01 MTR_5g090910 41.497 147 81 4 66 209 8 152 2.27e-29 107
MsG0180004881.01.T01 MTR_1g088840 37.500 112 70 0 99 210 3 114 1.64e-24 94.0
MsG0180004881.01.T01 MTR_4g134770 26.316 228 144 5 1 219 1 213 2.99e-14 71.2
MsG0180004881.01.T01 MTR_3g058940 27.358 212 143 5 1 210 1 203 1.28e-13 69.3
MsG0180004881.01.T01 MTR_1g492670 42.188 64 37 0 147 210 2 65 2.62e-12 60.5
MsG0180004881.01.T01 MTR_2g005570 27.053 207 130 5 11 210 13 205 8.30e-12 63.9
MsG0180004881.01.T01 MTR_2g005570 27.184 206 131 5 11 210 13 205 1.09e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004881.01.T01 AT3G62760 56.221 217 95 0 1 217 1 217 6.52e-96 279
MsG0180004881.01.T01 AT1G02930 41.827 208 119 1 2 209 3 208 2.16e-58 183
MsG0180004881.01.T01 AT1G02930 41.827 208 119 1 2 209 3 208 2.16e-58 183
MsG0180004881.01.T01 AT1G02920 41.346 208 121 1 2 209 3 209 2.31e-56 178
MsG0180004881.01.T01 AT2G30860 43.721 215 118 3 1 214 1 213 3.38e-55 176
MsG0180004881.01.T01 AT2G47730 41.951 205 119 0 5 209 54 258 2.16e-54 175
MsG0180004881.01.T01 AT2G47730 41.951 205 119 0 5 209 54 258 2.16e-54 175
MsG0180004881.01.T01 AT4G02520 40.476 210 121 2 2 208 3 211 1.70e-53 171
MsG0180004881.01.T01 AT2G30870 43.541 209 115 3 1 208 1 207 8.15e-53 169
MsG0180004881.01.T01 AT2G02930 39.524 210 125 1 2 209 3 212 1.01e-52 169
MsG0180004881.01.T01 AT1G02950 35.437 206 133 0 1 206 23 228 2.78e-49 161
MsG0180004881.01.T01 AT1G02950 35.437 206 133 0 1 206 23 228 2.78e-49 161
MsG0180004881.01.T01 AT3G03190 37.619 210 129 2 1 209 1 209 4.17e-49 160
MsG0180004881.01.T01 AT1G02950 35.437 206 133 0 1 206 35 240 4.78e-49 161
MsG0180004881.01.T01 AT1G02950 35.961 203 130 0 4 206 28 230 5.81e-49 160
MsG0180004881.01.T01 AT1G49860 41.500 200 112 3 14 209 16 214 1.14e-47 157
MsG0180004881.01.T01 AT5G17220 33.333 210 138 2 1 209 1 209 5.91e-46 152
MsG0180004881.01.T01 AT1G02940 36.408 206 131 0 4 209 40 245 1.83e-45 152
MsG0180004881.01.T01 AT1G02940 36.408 206 131 0 4 209 40 245 1.83e-45 152
MsG0180004881.01.T01 AT1G02940 36.408 206 131 0 4 209 53 258 2.36e-45 152
MsG0180004881.01.T01 AT1G02940 36.408 206 131 0 4 209 65 270 2.78e-45 152
MsG0180004881.01.T01 AT2G30860 40.000 155 91 2 1 155 1 153 3.78e-32 115
MsG0180004881.01.T01 AT1G02950 30.070 143 100 0 64 206 60 202 7.39e-26 100
MsG0180004881.01.T01 AT1G09640 26.415 212 146 5 1 210 1 204 1.13e-11 63.9
MsG0180004881.01.T01 AT1G57720 26.606 218 138 7 1 210 1 204 1.22e-11 63.5
MsG0180004881.01.T01 AT1G57720 26.606 218 138 7 1 210 1 204 1.22e-11 63.5
MsG0180004881.01.T01 AT1G09640 26.415 212 146 5 1 210 1 204 1.50e-11 62.8

Find 43 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTGACCTGTTTGGTTCT+TGG 0.194862 1:-83923566 None:intergenic
ATCATGGCACTCAAGATTTA+TGG 0.277244 1:+83922776 None:intergenic
ACAAAGTATTCATAGATAAT+TGG 0.322317 1:-83923528 None:intergenic
TTCAGCTACATATGAGGTTA+TGG 0.327058 1:-83923394 None:intergenic
GATGATGATCTCACCCTCTT+TGG 0.366830 1:+83923028 MsG0180004881.01.T01:CDS
CTTTCAATCACTGACCTGTT+TGG 0.367204 1:-83923573 None:intergenic
CAATGAACGGATTCATGTTA+AGG 0.371100 1:+83923746 MsG0180004881.01.T01:CDS
TTTGGTTCTTGGCCTTGAAA+TGG 0.382658 1:-83923555 None:intergenic
AAAATGGACCTGATTTGATT+AGG 0.390014 1:+83923427 MsG0180004881.01.T01:CDS
TACTTTGTGGTTCCATTTCA+AGG 0.391654 1:+83923543 MsG0180004881.01.T01:CDS
CTTACGTTCTTGTTGAGAAA+AGG 0.412482 1:-83922908 None:intergenic
GGTGGGAAGATATATCTTCT+AGG 0.418080 1:+83923772 MsG0180004881.01.T01:CDS
TCTTTGTTCATGCAGCCCTT+TGG 0.422040 1:+83922986 MsG0180004881.01.T01:intron
AGCACAACAAAATACCTTGC+AGG 0.425763 1:+83923645 MsG0180004881.01.T01:CDS
AATTATCTATGAATACTTTG+TGG 0.442647 1:+83923530 MsG0180004881.01.T01:CDS
TGACACAAAAGAAGCTTCAT+TGG 0.444080 1:+83923455 MsG0180004881.01.T01:CDS
TAGATAATTGGTGTGACTGC+AGG 0.445936 1:-83923516 None:intergenic
GTTGTGGGAGGTATGACCTT+TGG 0.467331 1:+83923810 MsG0180004881.01.T01:CDS
AGATCATCATCTTCAAGTAC+TGG 0.467902 1:-83923016 None:intergenic
GGCACTCAAGATTTATGGTC+TGG 0.469062 1:+83922781 MsG0180004881.01.T01:CDS
TTCAAGGCCAAGAACCAAAC+AGG 0.470857 1:+83923559 MsG0180004881.01.T01:CDS
TCATTGTGCCTAATCAAATC+AGG 0.483923 1:-83923435 None:intergenic
AACGGATTCATGTTAAGGCT+TGG 0.491423 1:+83923751 MsG0180004881.01.T01:CDS
GCACAACAAAATACCTTGCA+GGG 0.513385 1:+83923646 MsG0180004881.01.T01:CDS
ACGAGTGGTGTTTGTGGACA+TGG 0.514578 1:-83922804 None:intergenic
CATGGCACGAGTGGTGTTTG+TGG 0.516433 1:-83922810 None:intergenic
AATAGTGAGTTGAAGATATG+TGG 0.530360 1:-83923695 None:intergenic
GATTCATGTTAAGGCTTGGT+GGG 0.536813 1:+83923755 MsG0180004881.01.T01:CDS
GGATTCATGTTAAGGCTTGG+TGG 0.539482 1:+83923754 MsG0180004881.01.T01:CDS
AGATAATTGGTGTGACTGCA+GGG 0.554379 1:-83923515 None:intergenic
AAAAGAAAGCATACCAAAGA+GGG 0.568007 1:-83923041 None:intergenic
TCAGAAGCAAACACATTGAC+TGG 0.571377 1:-83922872 None:intergenic
ACGTTCTTGTTGAGAAAAGG+AGG 0.590052 1:-83922905 None:intergenic
AGTACTGGAATCAAACCAAA+GGG 0.590562 1:-83923001 None:intergenic
AAGCTTCATTGGTCAAAATG+TGG 0.591373 1:+83923466 MsG0180004881.01.T01:CDS
GTGCTTCAATGATCAATGAA+CGG 0.607609 1:+83923733 MsG0180004881.01.T01:CDS
ATTGATCATTGAAGCACAAG+GGG 0.612739 1:-83923727 None:intergenic
AAGTACTGGAATCAAACCAA+AGG 0.617639 1:-83923002 None:intergenic
TCATTGATCATTGAAGCACA+AGG 0.633784 1:-83923729 None:intergenic
CATTGATCATTGAAGCACAA+GGG 0.636560 1:-83923728 None:intergenic
CACAACAAAATACCTTGCAG+GGG 0.673341 1:+83923647 MsG0180004881.01.T01:CDS
TAGACATATCATGGCACGAG+TGG 0.683620 1:-83922819 None:intergenic
ATTGGTCAAAATGTGGACAG+AGG 0.694356 1:+83923473 MsG0180004881.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTCGAATAAACTTAAA+GGG - Chr1:83923106-83923125 None:intergenic 15.0%
!!! TATTTTTCGAATAAACTTAA+AGG - Chr1:83923107-83923126 None:intergenic 15.0%
!! AATTATCTATGAATACTTTG+TGG + Chr1:83923530-83923549 MsG0180004881.01.T01:CDS 20.0%
!! GAATTAGTAACAAAAATTAG+AGG - Chr1:83923239-83923258 None:intergenic 20.0%
!!! ACAAAGTATTCATAGATAAT+TGG - Chr1:83923531-83923550 None:intergenic 20.0%
! AATAGGTTACATATCATTAC+AGG - Chr1:83923337-83923356 None:intergenic 25.0%
! AATCAAGCAAAAATACTACT+AGG + Chr1:83923154-83923173 MsG0180004881.01.T01:intron 25.0%
! ATATGTAGCTGAAAAATACA+AGG + Chr1:83923404-83923423 MsG0180004881.01.T01:CDS 25.0%
! ATCATTACAGGTTCAAAAAA+TGG - Chr1:83923325-83923344 None:intergenic 25.0%
!! TAACCTATTTTATGTGACTA+TGG + Chr1:83923348-83923367 MsG0180004881.01.T01:intron 25.0%
!! TTTTTCATGTAGACATATCA+TGG - Chr1:83922831-83922850 None:intergenic 25.0%
AGTCCATAGTCACATAAAAT+AGG - Chr1:83923354-83923373 None:intergenic 30.0%
GCTGAAAAATACAAGGAAAA+TGG + Chr1:83923411-83923430 MsG0180004881.01.T01:CDS 30.0%
TTTCGAATAAACTTAAAGGG+TGG - Chr1:83923103-83923122 None:intergenic 30.0%
! AAAAAGAAAGCATACCAAAG+AGG - Chr1:83923045-83923064 None:intergenic 30.0%
! AAAAGAAAGCATACCAAAGA+GGG - Chr1:83923044-83923063 None:intergenic 30.0%
! AAAATGGACCTGATTTGATT+AGG + Chr1:83923427-83923446 MsG0180004881.01.T01:CDS 30.0%
! AATAGTGAGTTGAAGATATG+TGG - Chr1:83923698-83923717 None:intergenic 30.0%
!! GTATTTTTCAGCTACATATG+AGG - Chr1:83923403-83923422 None:intergenic 30.0%
AAGCTTCATTGGTCAAAATG+TGG + Chr1:83923466-83923485 MsG0180004881.01.T01:CDS 35.0%
AGATCATCATCTTCAAGTAC+TGG - Chr1:83923019-83923038 None:intergenic 35.0%
ATTGATCATTGAAGCACAAG+GGG - Chr1:83923730-83923749 None:intergenic 35.0%
CATTGATCATTGAAGCACAA+GGG - Chr1:83923731-83923750 None:intergenic 35.0%
CTTACGTTCTTGTTGAGAAA+AGG - Chr1:83922911-83922930 None:intergenic 35.0%
GTGCTTCAATGATCAATGAA+CGG + Chr1:83923733-83923752 MsG0180004881.01.T01:CDS 35.0%
TACTTTGTGGTTCCATTTCA+AGG + Chr1:83923543-83923562 MsG0180004881.01.T01:CDS 35.0%
TCATTGATCATTGAAGCACA+AGG - Chr1:83923732-83923751 None:intergenic 35.0%
TCATTGTGCCTAATCAAATC+AGG - Chr1:83923438-83923457 None:intergenic 35.0%
TCTGCTTTCCAAAAAGTTGT+GGG + Chr1:83923795-83923814 MsG0180004881.01.T01:CDS 35.0%
TGACACAAAAGAAGCTTCAT+TGG + Chr1:83923455-83923474 MsG0180004881.01.T01:CDS 35.0%
TTCAGCTACATATGAGGTTA+TGG - Chr1:83923397-83923416 None:intergenic 35.0%
! AGTACTGGAATCAAACCAAA+GGG - Chr1:83923004-83923023 None:intergenic 35.0%
! CAATGAACGGATTCATGTTA+AGG + Chr1:83923746-83923765 MsG0180004881.01.T01:CDS 35.0%
! TTTTTGATCACTCTGACCAA+AGG - Chr1:83923829-83923848 None:intergenic 35.0%
!! AAGTACTGGAATCAAACCAA+AGG - Chr1:83923005-83923024 None:intergenic 35.0%
!! GTATTTTGTTGTGCTTAGCT+TGG - Chr1:83923640-83923659 None:intergenic 35.0%
ACGTTCTTGTTGAGAAAAGG+AGG - Chr1:83922908-83922927 None:intergenic 40.0%
AGATAATTGGTGTGACTGCA+GGG - Chr1:83923518-83923537 None:intergenic 40.0%
AGCACAACAAAATACCTTGC+AGG + Chr1:83923645-83923664 MsG0180004881.01.T01:CDS 40.0%
ATTGGTCAAAATGTGGACAG+AGG + Chr1:83923473-83923492 MsG0180004881.01.T01:CDS 40.0%
CACAACAAAATACCTTGCAG+GGG + Chr1:83923647-83923666 MsG0180004881.01.T01:CDS 40.0%
CTTTCAATCACTGACCTGTT+TGG - Chr1:83923576-83923595 None:intergenic 40.0%
GACTATAAAAATCCCCTGCA+AGG - Chr1:83923662-83923681 None:intergenic 40.0%
GCACAACAAAATACCTTGCA+GGG + Chr1:83923646-83923665 MsG0180004881.01.T01:CDS 40.0%
GCATGATTAGTGGTCTAGAT+TGG - Chr1:83923270-83923289 None:intergenic 40.0%
GGTGGGAAGATATATCTTCT+AGG + Chr1:83923772-83923791 MsG0180004881.01.T01:CDS 40.0%
GTCTGCTTTCCAAAAAGTTG+TGG + Chr1:83923794-83923813 MsG0180004881.01.T01:CDS 40.0%
TAGATAATTGGTGTGACTGC+AGG - Chr1:83923519-83923538 None:intergenic 40.0%
TCAGAAGCAAACACATTGAC+TGG - Chr1:83922875-83922894 None:intergenic 40.0%
TGACATGCATGCATGATTAG+TGG - Chr1:83923280-83923299 None:intergenic 40.0%
! AACGGATTCATGTTAAGGCT+TGG + Chr1:83923751-83923770 MsG0180004881.01.T01:CDS 40.0%
! GATTCATGTTAAGGCTTGGT+GGG + Chr1:83923755-83923774 MsG0180004881.01.T01:CDS 40.0%
! TCATACCTCCCACAACTTTT+TGG - Chr1:83923806-83923825 None:intergenic 40.0%
!! TTTGGTTCTTGGCCTTGAAA+TGG - Chr1:83923558-83923577 None:intergenic 40.0%
GATGATGATCTCACCCTCTT+TGG + Chr1:83923028-83923047 MsG0180004881.01.T01:CDS 45.0%
GCTTTCCAAAAAGTTGTGGG+AGG + Chr1:83923798-83923817 MsG0180004881.01.T01:CDS 45.0%
GGATTCATGTTAAGGCTTGG+TGG + Chr1:83923754-83923773 MsG0180004881.01.T01:CDS 45.0%
GGCACTCAAGATTTATGGTC+TGG + Chr1:83922781-83922800 MsG0180004881.01.T01:CDS 45.0%
TAGACATATCATGGCACGAG+TGG - Chr1:83922822-83922841 None:intergenic 45.0%
TCTTTGTTCATGCAGCCCTT+TGG + Chr1:83922986-83923005 MsG0180004881.01.T01:intron 45.0%
TTCAAGGCCAAGAACCAAAC+AGG + Chr1:83923559-83923578 MsG0180004881.01.T01:CDS 45.0%
!! TCACTGACCTGTTTGGTTCT+TGG - Chr1:83923569-83923588 None:intergenic 45.0%
ACGAGTGGTGTTTGTGGACA+TGG - Chr1:83922807-83922826 None:intergenic 50.0%
! GTTGTGGGAGGTATGACCTT+TGG + Chr1:83923810-83923829 MsG0180004881.01.T01:CDS 50.0%
CATGGCACGAGTGGTGTTTG+TGG - Chr1:83922813-83922832 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 83922779 83923851 83922779 ID=MsG0180004881.01;Name=MsG0180004881.01
Chr1 mRNA 83922779 83923851 83922779 ID=MsG0180004881.01.T01;Parent=MsG0180004881.01;Name=MsG0180004881.01.T01;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|1|1|3|0|220
Chr1 exon 83922779 83922925 83922779 ID=MsG0180004881.01.T01:exon:9070;Parent=MsG0180004881.01.T01
Chr1 exon 83923001 83923049 83923001 ID=MsG0180004881.01.T01:exon:9071;Parent=MsG0180004881.01.T01
Chr1 exon 83923385 83923851 83923385 ID=MsG0180004881.01.T01:exon:9072;Parent=MsG0180004881.01.T01
Chr1 CDS 83922779 83922925 83922779 ID=MsG0180004881.01.T01:cds;Parent=MsG0180004881.01.T01
Chr1 CDS 83923001 83923049 83923001 ID=MsG0180004881.01.T01:cds;Parent=MsG0180004881.01.T01
Chr1 CDS 83923385 83923851 83923385 ID=MsG0180004881.01.T01:cds;Parent=MsG0180004881.01.T01
Gene Sequence

>MsG0180004881.01.T01

ATGGCACTCAAGATTTATGGTCTGGCCATGTCCACAAACACCACTCGTGCCATGATATGTCTACATGAAAAAGAAGTAGATTTTGAGCTTATTCCAGTCAATGTGTTTGCTTCTGAGCATAAACAGCCTCCTTTTCTCAACAAGAACCCCTTTGGTTTGATTCCAGTACTTGAAGATGATGATCTCACCCTCTTTGAGTCAAGAGCCATAACCTCATATGTAGCTGAAAAATACAAGGAAAATGGACCTGATTTGATTAGGCACAATGACACAAAAGAAGCTTCATTGGTCAAAATGTGGACAGAGGTAGAGTCTCACTACTATGACCCTGCAGTCACACCAATTATCTATGAATACTTTGTGGTTCCATTTCAAGGCCAAGAACCAAACAGGTCAGTGATTGAAAGTAACATTGAGAAGCTGAAGATTGTGCTTGATGTGTATGAGACCAAGCTAAGCACAACAAAATACCTTGCAGGGGATTTTTATAGTCTTGCTGATCTTAGCCACATATCTTCAACTCACTATTTTATGCAGACCCCTTGTGCTTCAATGATCAATGAACGGATTCATGTTAAGGCTTGGTGGGAAGATATATCTTCTAGGTCTGCTTTCCAAAAAGTTGTGGGAGGTATGACCTTTGGTCAGAGTGATCAAAAATGA

Protein sequence

>MsG0180004881.01.T01

MALKIYGLAMSTNTTRAMICLHEKEVDFELIPVNVFASEHKQPPFLNKNPFGLIPVLEDDDLTLFESRAITSYVAEKYKENGPDLIRHNDTKEASLVKMWTEVESHYYDPAVTPIIYEYFVVPFQGQEPNRSVIESNIEKLKIVLDVYETKLSTTKYLAGDFYSLADLSHISSTHYFMQTPCASMINERIHVKAWWEDISSRSAFQKVVGGMTFGQSDQK*