AlfalfaGEDB | MsG0280009933.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009933.01

KEGG: K16055
GO: GO:0003824 GO:0005992
PFAM: PF00982 PF02358

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009933.01.T01	XP_024632343.1	90.55	963	32	5	1	905	1	962	0	1769

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009933.01.T01	Q9SYM4	66.254	969	223	11	9	902	1	940	0	1239

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009933.01.T01	A0A396JEH8	90.550	963	32	5	1	905	1	962	0.0	1769

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004277.01	MsG0280009933.01	0.805908	1.063653e-49	5.695708e-47
MsG0180004988.01	MsG0280009933.01	0.801936	7.115375e-49	3.441125e-46
MsG0280009933.01	MsG0380014794.01	0.826960	2.035695e-54	1.938121e-51
MsG0280009933.01	MsG0380015486.01	0.827113	1.871278e-54	1.789454e-51
MsG0280009933.01	MsG0580028719.01	0.817114	3.899417e-52	2.814644e-49
MsG0280009933.01	MsG0880044333.01	0.801369	9.300005e-49	4.433759e-46
MsG0280009933.01	MsG0880046406.01	0.807763	4.312670e-50	2.422426e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009933.01.T01	MTR_2g073260	90.456	964	32	6	1	905	1	963	0.0	1766
MsG0280009933.01.T01	MTR_0034s0170	68.724	956	196	10	9	898	1	919	0.0	1273
MsG0280009933.01.T01	MTR_8g087930	62.134	478	153	7	443	902	56	523	0.0	578
MsG0280009933.01.T01	MTR_8g087910	79.819	332	60	1	115	446	70	394	0.0	570
MsG0280009933.01.T01	MTR_1g032730	34.519	759	377	24	120	777	61	800	1.65e-126	402
MsG0280009933.01.T01	MTR_1g109620	34.570	755	376	26	120	776	58	792	1.13e-124	398
MsG0280009933.01.T01	MTR_8g105740	34.211	760	379	25	120	777	59	799	8.00e-123	393
MsG0280009933.01.T01	MTR_4g080160	31.921	755	394	22	122	778	67	799	3.75e-121	389
MsG0280009933.01.T01	MTR_8g063790	32.941	765	377	26	120	777	52	787	1.00e-116	377
MsG0280009933.01.T01	MTR_4g129270	32.543	759	390	23	120	777	55	792	1.24e-115	374

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009933.01.T01	AT1G78580	66.254	969	223	11	9	902	1	940	0.0	1239
MsG0280009933.01.T01	AT1G78580	66.254	969	223	11	9	902	1	940	0.0	1239
MsG0280009933.01.T01	AT1G78580	66.254	969	223	11	9	902	1	940	0.0	1239
MsG0280009933.01.T01	AT1G78580	66.254	969	223	11	9	902	1	940	0.0	1239
MsG0280009933.01.T01	AT1G16980	60.414	725	227	5	112	780	3	723	0.0	905
MsG0280009933.01.T01	AT4G27550	60.388	722	223	6	120	782	3	720	0.0	884
MsG0280009933.01.T01	AT4G27550	59.781	731	222	7	120	782	3	729	0.0	876
MsG0280009933.01.T01	AT1G16980	59.344	610	197	4	112	674	3	608	0.0	754
MsG0280009933.01.T01	AT4G17770	33.554	754	386	23	120	777	59	793	1.45e-124	397
MsG0280009933.01.T01	AT4G17770	33.554	754	386	23	120	777	59	793	1.45e-124	397

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Find 220 sgRNAs with CRISPR-Local

Find 621 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCTTGGAAGAGAATG+AGG	0.435584	2:-61217167	MsG0280009933.01.T01:CDS
AAAGGGAAGCTTCCCTTGTA+TGG	0.415736	2:-61212693	MsG0280009933.01.T01:CDS
AAATTGATATTCAACTTCAA+TGG	0.475514	2:-61221130	MsG0280009933.01.T01:exon
AACCCACACTGCGCAGGAAT+GGG	0.490104	2:-61214525	MsG0280009933.01.T01:CDS
AACTAATCGATTGCTTATTT+TGG	0.140569	2:-61213947	MsG0280009933.01.T01:intron
AAGAGAGAGAGAAGCGACAT+AGG	0.520818	2:-61214568	MsG0280009933.01.T01:CDS
AAGTCAGCAAATCAAAGCAA+GGG	0.617111	2:-61211910	MsG0280009933.01.T01:CDS
AATAGTGATCCGGGTCGTGT+TGG	0.515868	2:-61221057	MsG0280009933.01.T01:CDS
AATGCCAGAACAACTAAACA+TGG	0.613238	2:-61212872	MsG0280009933.01.T01:CDS
AATGGATATGAAAGGGAAGA+TGG	0.497447	2:-61220787	MsG0280009933.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTCTTGGAAGAGAATG+AGG	-	Chr2:61215840-61215859	MsG0280009933.01.T01:intron	30.0%
AAATTACCGAAAGAGTAACA+AGG	+	Chr2:61217600-61217619	None:intergenic	30.0%
AACAAAAGTTCATCGAAAAC+TGG	-	Chr2:61216808-61216827	MsG0280009933.01.T01:intron	30.0%
AAGTCCAAGGTAATGAAAAA+GGG	+	Chr2:61213034-61213053	None:intergenic	30.0%
AAGTTTGTGGTTTACAATGA+GGG	-	Chr2:61218562-61218581	MsG0280009933.01.T01:intron	30.0%
AATAGCAAATATCCGCAAAT+CGG	+	Chr2:61214971-61214990	None:intergenic	30.0%
AATTTCAGAAGATGGAAATG+GGG	+	Chr2:61213262-61213281	None:intergenic	30.0%
ACAATCATAGATGTTGAGTT+TGG	-	Chr2:61220423-61220442	MsG0280009933.01.T01:intron	30.0%
AGAAAACTCTATGTAACCTA+GGG	+	Chr2:61212672-61212691	None:intergenic	30.0%
AGAAAGAAAAAAAGGCTTCA+AGG	-	Chr2:61214283-61214302	MsG0280009933.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	61211457	61221572	61211457	ID=MsG0280009933.01;Name=MsG0280009933.01
Chr2	mRNA	61211457	61221572	61211457	ID=MsG0280009933.01.T01;Parent=MsG0280009933.01;Name=MsG0280009933.01.T01;_AED=0.41;_eAED=0.41;_QI=416\|0.86\|0.93\|1\|0.93\|0.87\|16\|212\|905
Chr2	exon	61211457	61212073	61211457	ID=MsG0280009933.01.T01:exon:13621;Parent=MsG0280009933.01.T01
Chr2	exon	61212264	61212362	61212264	ID=MsG0280009933.01.T01:exon:13620;Parent=MsG0280009933.01.T01
Chr2	exon	61212466	61212596	61212466	ID=MsG0280009933.01.T01:exon:13619;Parent=MsG0280009933.01.T01
Chr2	exon	61212677	61212737	61212677	ID=MsG0280009933.01.T01:exon:13618;Parent=MsG0280009933.01.T01
Chr2	exon	61212852	61212974	61212852	ID=MsG0280009933.01.T01:exon:13617;Parent=MsG0280009933.01.T01
Chr2	exon	61213044	61213211	61213044	ID=MsG0280009933.01.T01:exon:13616;Parent=MsG0280009933.01.T01
Chr2	exon	61213948	61214065	61213948	ID=MsG0280009933.01.T01:exon:13615;Parent=MsG0280009933.01.T01
Chr2	exon	61214509	61214696	61214509	ID=MsG0280009933.01.T01:exon:13614;Parent=MsG0280009933.01.T01
Chr2	exon	61214840	61214940	61214840	ID=MsG0280009933.01.T01:exon:13613;Parent=MsG0280009933.01.T01
Chr2	exon	61217104	61217254	61217104	ID=MsG0280009933.01.T01:exon:13612;Parent=MsG0280009933.01.T01
Chr2	exon	61218959	61219060	61218959	ID=MsG0280009933.01.T01:exon:13611;Parent=MsG0280009933.01.T01
Chr2	exon	61219225	61219339	61219225	ID=MsG0280009933.01.T01:exon:13610;Parent=MsG0280009933.01.T01
Chr2	exon	61219686	61220074	61219686	ID=MsG0280009933.01.T01:exon:13609;Parent=MsG0280009933.01.T01
Chr2	exon	61220199	61220296	61220199	ID=MsG0280009933.01.T01:exon:13608;Parent=MsG0280009933.01.T01
Chr2	exon	61220665	61221378	61220665	ID=MsG0280009933.01.T01:exon:13607;Parent=MsG0280009933.01.T01
Chr2	exon	61221402	61221572	61221402	ID=MsG0280009933.01.T01:exon:13606;Parent=MsG0280009933.01.T01
Chr2	five_prime_UTR	61221402	61221572	61221402	ID=MsG0280009933.01.T01:five_prime_utr;Parent=MsG0280009933.01.T01
Chr2	five_prime_UTR	61221134	61221378	61221134	ID=MsG0280009933.01.T01:five_prime_utr;Parent=MsG0280009933.01.T01
Chr2	CDS	61220665	61221133	61220665	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61220199	61220296	61220199	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61219686	61220074	61219686	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61219225	61219339	61219225	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61218959	61219060	61218959	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61217104	61217254	61217104	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61214840	61214940	61214840	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61214509	61214696	61214509	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61213948	61214065	61213948	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61213044	61213211	61213044	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61212852	61212974	61212852	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61212677	61212737	61212677	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61212466	61212596	61212466	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61212264	61212362	61212264	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	CDS	61211669	61212073	61211669	ID=MsG0280009933.01.T01:cds;Parent=MsG0280009933.01.T01
Chr2	three_prime_UTR	61211457	61211668	61211457	ID=MsG0280009933.01.T01:three_prime_utr;Parent=MsG0280009933.01.T01

Gene Sequence

>MsG0280009933.01.T01

ATGGTGGGGTTCCAGAGTGAACATATGCCTGGAAACAAGTTTAATGGTAATTATAATAGTGATCCGGGTCGTGTTGGACGACTTGTGAAAATTAGAGAACAGATAAAAAGCAGCAGGGCATCATCTGATACTGTTCTTATTAACAATAATAACAATATTAATAGCAATAATAATAATAATGTTAATAATAACTATAATGATAGTGATAAACATGTGGTTACTGATTCTTTTGAGCATGACCAATGCTTCAAAGAATTTAATAACTCTGGTGCTTCCCATGTTGAACGATTCCTGGAAGGTGCCGCAGCAGCAAAGGCTCTTAGTAATGGATATGAAAGGGAAGATGGAAAACCTGTTAGACAACGGCTTTTGGTTGTGGCTAATAGATTGCCAGTTTCAGCTGTTAGGAAAGGCGAGGATACTTGGTCTCTGGAGATCAGTGCTGGCGGCTTGGTCAGCGCTCTTTTAGGTGTGAAGGATTTTGAAGCGAGGTGGATAGGTTGGGCCGGTGTCAATGTGGCGGATGCGGTTGGTCAGAAGACACTCACTGAAGCATTAGCTGAAAAGAGGTGTATCCCAGTATTTCTTACTGAAGAGATTGTTCATCAGTACTATAATGGCTATTGCAATAACATTTTGTGGCCCCTTTTTCATTACCTTGGACTTCCACAAGAAGATCGTCTAGCGACAACACGTGGATTCCAGTCTCAGTTTGATTCGTATCAGGAGGCAAACCAAATGTTTGCGGATGTTGTAAATCAGGTCTATGAAGAGGGAGATATTGTTTGGTGTCATGATTATCATCTCATGTTTCTACCTCAATGCTTAAAGAAATTTAACAGCAAAATGAAAGTTGGTTGGTTTCTCCATACCCCATTTCCATCTTCTGAAATTCATAGGACACTGCCATCTCGTTCAGAACTCTTGCGTGCAGTTCTTGCTGCTGATTTGGTTGGTTTCCACACATATGATTATGCACGACACTTTGTTAGTGCGTGTACTCGCATTCTTGGACTTGAGGCCACGCCAGAAGGGGTTGAGGATCAAGGGAAACTGACTCGAGTTGCTGCGTTTCCAATTGGTATAGACTCGGATCGATTCATACATGCACTTGACCATCCTAAAGTCCAAGCTCCATTCAAAGACTTAGTAGAAAGATTTAAAGGCAGAAAGGTAATGTTAGGTGTTGATCGCCTTGATATGATTAAAGGAATTCCTCAAAAACTTCTTGCTTTTGAAAAATTCTTGGAAGAGAATGAGGATTGGCGAGAAAAGGTCGTTCTGCTTCAAATTGCTGTGCCAACAAGAACAGATGTTCCCGAGTATGTTGCACTTGTCACGTCTTTGAGGGATGGAATGAATCTTGTCAGTTACGAGTTTGTAGCCTGTCAAGAAAAAAAGAAGGGGGTTCTCATTCTTAGTGAATTTGCTGGTGCTGCGCAGTCTCTTGGGGCTGGGGCACTTCTGGTCAACCCCTGGAACATCTCAAAAGTTGCCGAAGCAATTGCCAAAGCTCTGAACATGCCATCAGAAGAGAGAGAGAAGCGACATAGGCACAACTTCCATCACGTAACAACCCACACTGCGCAGGAATGGGCAGAAACTTTTGTGAGTGAGCTAAATGATACTGTTGTTGAGGCACAGTTAAGGACAAAACAAGTTCCACCACGGCTCGATACCAGGAAGACAATTCAACAATATCTGAAGTCAACTAATCGATTGCTTATTTTGGGATTCAATGGAACTTTAACTGAGCAAGTTGAGAGAAAAGGTGATCAACTGAAAGAAATGGAACTCTCAGTCCATCCAGAATTGAAACAACCGTTGTCACAACTTTGCAATGACCCAAATACTACAGTTGTCGTTCTTAGTGGAAGTGGTAGAACACTTCTAGATGAAAACTTCAAAGAATATAACACTTGGCTAGCAGCAGAGAATGGGATGTTTTTAAACCCTTCAAATGGAGAATGGATGACTACAATGCCAGAACAACTAAACATGGAATGGGTTGACAGTGTAAAGAACTCCTCGGTCGTATTTGAAGAAAGGGAAGCTTCCCTTGTATGGAATTACAGACATGCAGATGTTGAGTTTGGGAAACTTCAAGCAAGGGACATGCTCCAACATCTCTGCACAGGTCCAATTTCAAATGCATCAGTTGAAGTTGTTCAAGGGAGCCGATCAGTTGAGGTGCGAGCTGCTGGTGTTACAAAGGGAGCAGCCATTGACCGCATCCTGGGGGAGATTGTTCACAGTAAATCGATGACTACACCAATTGATTATGTTCTATGTATAGGACATTTTCTGGCAAAGGATGAAGATATATATGACTTTTTTGAGCCAGAGCCTTCTTCTTTTGGTGCAAGTCTTCAGCGAAGCAAGTTATCAGATGCAGCTAAGTTTCCCAATGAGAAGGTATCACCTATGAAGATCCCATCAATTAAAAATGGATTCAAGTCAGCAAATCAAAGCAAGGGACAGCGACCTATCTCAAATTCAGAGAAGAAAACAGCAAATAATGTCAACCGCACGGCACGGAAGTGTAAATCACCTTTGCCAGAAAAGATCTCTTGGAATGTCCTTGACCTCAAGAAGGAGAATTACTTCTCGTGTGCTGTTGGAAGAATTCAAACAAATGCTCGATTTACACTTCCATCGTCTGACGAGGTTGCTGAATTTCTGAAGAAACTTGCACATGCATCTTCTTGTGATTACTGA

Protein sequence

>MsG0280009933.01.T01

MVGFQSEHMPGNKFNGNYNSDPGRVGRLVKIREQIKSSRASSDTVLINNNNNINSNNNNNVNNNYNDSDKHVVTDSFEHDQCFKEFNNSGASHVERFLEGAAAAKALSNGYEREDGKPVRQRLLVVANRLPVSAVRKGEDTWSLEISAGGLVSALLGVKDFEARWIGWAGVNVADAVGQKTLTEALAEKRCIPVFLTEEIVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRGFQSQFDSYQEANQMFADVVNQVYEEGDIVWCHDYHLMFLPQCLKKFNSKMKVGWFLHTPFPSSEIHRTLPSRSELLRAVLAADLVGFHTYDYARHFVSACTRILGLEATPEGVEDQGKLTRVAAFPIGIDSDRFIHALDHPKVQAPFKDLVERFKGRKVMLGVDRLDMIKGIPQKLLAFEKFLEENEDWREKVVLLQIAVPTRTDVPEYVALVTSLRDGMNLVSYEFVACQEKKKGVLILSEFAGAAQSLGAGALLVNPWNISKVAEAIAKALNMPSEEREKRHRHNFHHVTTHTAQEWAETFVSELNDTVVEAQLRTKQVPPRLDTRKTIQQYLKSTNRLLILGFNGTLTEQVERKGDQLKEMELSVHPELKQPLSQLCNDPNTTVVVLSGSGRTLLDENFKEYNTWLAAENGMFLNPSNGEWMTTMPEQLNMEWVDSVKNSSVVFEEREASLVWNYRHADVEFGKLQARDMLQHLCTGPISNASVEVVQGSRSVEVRAAGVTKGAAIDRILGEIVHSKSMTTPIDYVLCIGHFLAKDEDIYDFFEPEPSSFGASLQRSKLSDAAKFPNEKVSPMKIPSIKNGFKSANQSKGQRPISNSEKKTANNVNRTARKCKSPLPEKISWNVLDLKKENYFSCAVGRIQTNARFTLPSSDEVAEFLKKLAHASSCDY*