Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010571.01.T01 | XP_003596929.2 | 96.947 | 393 | 12 | 0 | 1 | 393 | 4 | 396 | 0 | 789 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010571.01.T01 | Q8VWF6 | 67.098 | 386 | 125 | 1 | 7 | 392 | 6 | 389 | 0 | 556 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010571.01.T01 | G7IRH8 | 96.947 | 393 | 12 | 0 | 1 | 393 | 4 | 396 | 0.0 | 789 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280008419.01 | MsG0280010571.01 | PPI |
| MsG0780040415.01 | MsG0280010571.01 | PPI |
| MsG0280008419.01 | MsG0280010571.01 | PPI |
| MsG0280008419.01 | MsG0280010571.01 | PPI |
| MsG0780040414.01 | MsG0280010571.01 | PPI |
| MsG0280011115.01 | MsG0280010571.01 | PPI |
| MsG0280008418.01 | MsG0280010571.01 | PPI |
| MsG0780040417.01 | MsG0280010571.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010571.01.T01 | MTR_2g087780 | 96.947 | 393 | 12 | 0 | 1 | 393 | 4 | 396 | 0.0 | 789 |
| MsG0280010571.01.T01 | MTR_4g050190 | 78.316 | 392 | 84 | 1 | 1 | 392 | 1 | 391 | 0.0 | 646 |
| MsG0280010571.01.T01 | MTR_7g090300 | 50.568 | 352 | 161 | 5 | 44 | 392 | 28 | 369 | 1.13e-116 | 344 |
| MsG0280010571.01.T01 | MTR_7g490350 | 50.568 | 352 | 161 | 5 | 44 | 392 | 28 | 369 | 6.16e-116 | 342 |
| MsG0280010571.01.T01 | MTR_2g099030 | 44.670 | 394 | 208 | 6 | 3 | 389 | 13 | 403 | 1.66e-108 | 325 |
| MsG0280010571.01.T01 | MTR_7g490330 | 48.673 | 339 | 124 | 4 | 57 | 392 | 34 | 325 | 2.13e-104 | 311 |
| MsG0280010571.01.T01 | MTR_5g091980 | 47.159 | 352 | 132 | 6 | 44 | 392 | 23 | 323 | 1.10e-99 | 299 |
| MsG0280010571.01.T01 | MTR_7g090310 | 42.943 | 333 | 182 | 6 | 66 | 391 | 54 | 385 | 4.24e-88 | 272 |
| MsG0280010571.01.T01 | MTR_7g090320 | 44.242 | 330 | 175 | 7 | 70 | 391 | 58 | 386 | 1.09e-87 | 271 |
| MsG0280010571.01.T01 | MTR_4g112670 | 37.313 | 335 | 177 | 7 | 60 | 390 | 29 | 334 | 7.59e-69 | 221 |
| MsG0280010571.01.T01 | MTR_4g112650 | 37.576 | 330 | 167 | 8 | 68 | 390 | 36 | 333 | 7.94e-64 | 207 |
| MsG0280010571.01.T01 | MTR_4g112630 | 36.667 | 330 | 161 | 6 | 68 | 390 | 36 | 324 | 5.29e-63 | 205 |
| MsG0280010571.01.T01 | MTR_2g058450 | 35.152 | 330 | 178 | 7 | 68 | 392 | 38 | 336 | 5.61e-57 | 190 |
| MsG0280010571.01.T01 | MTR_4g112620 | 32.110 | 327 | 151 | 6 | 68 | 390 | 40 | 299 | 5.12e-45 | 157 |
| MsG0280010571.01.T01 | MTR_8g074170 | 31.316 | 380 | 214 | 9 | 10 | 387 | 27 | 361 | 2.72e-42 | 152 |
| MsG0280010571.01.T01 | MTR_8g074210 | 31.926 | 379 | 215 | 10 | 10 | 388 | 27 | 362 | 3.64e-42 | 152 |
| MsG0280010571.01.T01 | MTR_8g074270 | 31.662 | 379 | 214 | 9 | 10 | 387 | 27 | 361 | 5.03e-42 | 151 |
| MsG0280010571.01.T01 | MTR_2g058410 | 34.375 | 256 | 140 | 4 | 136 | 389 | 1 | 230 | 6.95e-34 | 126 |
| MsG0280010571.01.T01 | MTR_0003s0530 | 32.039 | 206 | 115 | 3 | 180 | 384 | 2 | 183 | 3.24e-30 | 115 |
| MsG0280010571.01.T01 | MTR_2g042370 | 28.626 | 262 | 159 | 5 | 143 | 392 | 112 | 357 | 4.29e-30 | 119 |
| MsG0280010571.01.T01 | MTR_2g042420 | 29.762 | 252 | 168 | 7 | 143 | 392 | 105 | 349 | 2.93e-28 | 114 |
| MsG0280010571.01.T01 | MTR_2g042430 | 30.769 | 247 | 164 | 6 | 143 | 388 | 124 | 364 | 9.81e-28 | 112 |
| MsG0280010571.01.T01 | MTR_8g074320 | 42.063 | 126 | 69 | 2 | 133 | 255 | 95 | 219 | 2.09e-25 | 103 |
| MsG0280010571.01.T01 | MTR_1g075865 | 44.762 | 105 | 58 | 0 | 184 | 288 | 7 | 111 | 2.83e-25 | 100 |
| MsG0280010571.01.T01 | MTR_4g112840 | 37.500 | 96 | 60 | 0 | 205 | 300 | 56 | 151 | 1.37e-17 | 80.1 |
| MsG0280010571.01.T01 | MTR_2g058430 | 50.633 | 79 | 38 | 1 | 127 | 204 | 52 | 130 | 2.72e-15 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010571.01.T01 | AT1G08470 | 67.098 | 386 | 125 | 1 | 7 | 392 | 6 | 389 | 0.0 | 556 |
| MsG0280010571.01.T01 | AT5G22020 | 64.894 | 376 | 130 | 1 | 17 | 392 | 22 | 395 | 0.0 | 521 |
| MsG0280010571.01.T01 | AT5G22020 | 64.894 | 376 | 130 | 1 | 17 | 392 | 22 | 395 | 0.0 | 521 |
| MsG0280010571.01.T01 | AT5G22020 | 64.894 | 376 | 130 | 1 | 17 | 392 | 22 | 395 | 0.0 | 521 |
| MsG0280010571.01.T01 | AT3G57030 | 50.733 | 341 | 158 | 3 | 55 | 392 | 41 | 374 | 3.97e-121 | 356 |
| MsG0280010571.01.T01 | AT3G59530 | 43.544 | 395 | 204 | 6 | 1 | 389 | 13 | 394 | 4.36e-106 | 318 |
| MsG0280010571.01.T01 | AT3G59530 | 43.544 | 395 | 204 | 6 | 1 | 389 | 13 | 394 | 4.36e-106 | 318 |
| MsG0280010571.01.T01 | AT3G59530 | 43.544 | 395 | 204 | 6 | 1 | 389 | 13 | 394 | 4.36e-106 | 318 |
| MsG0280010571.01.T01 | AT3G57010 | 46.201 | 329 | 157 | 7 | 68 | 389 | 52 | 367 | 6.73e-93 | 284 |
| MsG0280010571.01.T01 | AT3G57020 | 45.289 | 329 | 160 | 7 | 68 | 389 | 51 | 366 | 6.47e-90 | 276 |
| MsG0280010571.01.T01 | AT2G41300 | 44.828 | 319 | 161 | 5 | 75 | 389 | 83 | 390 | 9.91e-90 | 276 |
| MsG0280010571.01.T01 | AT3G57020 | 44.073 | 329 | 150 | 7 | 68 | 389 | 51 | 352 | 2.03e-84 | 261 |
| MsG0280010571.01.T01 | AT2G41290 | 41.983 | 343 | 162 | 10 | 70 | 392 | 51 | 376 | 7.37e-80 | 250 |
| MsG0280010571.01.T01 | AT1G74010 | 37.538 | 333 | 162 | 12 | 66 | 392 | 33 | 325 | 2.96e-51 | 174 |
| MsG0280010571.01.T01 | AT1G74000 | 34.347 | 329 | 171 | 9 | 70 | 392 | 40 | 329 | 4.08e-49 | 169 |
| MsG0280010571.01.T01 | AT1G74020 | 34.242 | 330 | 172 | 11 | 69 | 392 | 37 | 327 | 6.58e-47 | 163 |
| MsG0280010571.01.T01 | AT3G51420 | 29.254 | 335 | 207 | 7 | 54 | 388 | 53 | 357 | 8.93e-36 | 135 |
| MsG0280010571.01.T01 | AT3G51440 | 32.959 | 267 | 155 | 6 | 53 | 318 | 52 | 295 | 5.12e-33 | 127 |
| MsG0280010571.01.T01 | AT3G51430 | 30.097 | 309 | 185 | 9 | 10 | 318 | 18 | 295 | 5.54e-32 | 124 |
| MsG0280010571.01.T01 | AT3G51450 | 30.566 | 265 | 162 | 5 | 54 | 318 | 53 | 295 | 2.50e-31 | 122 |
| MsG0280010571.01.T01 | AT3G51430 | 30.690 | 290 | 170 | 9 | 10 | 299 | 18 | 276 | 2.86e-31 | 122 |
Find 110 sgRNAs with CRISPR-Local
Find 149 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATTCAGTTGATACTTTC+TGG | 0.149122 | 2:+71670675 | MsG0280010571.01.T01:CDS |
| TACATAGCAGATGCATATTT+CGG | 0.221241 | 2:+71670372 | MsG0280010571.01.T01:CDS |
| GACATCTGCGGGAGGCCTTT+GGG | 0.272968 | 2:+71670318 | MsG0280010571.01.T01:CDS |
| GGACATCTGCGGGAGGCCTT+TGG | 0.299116 | 2:+71670317 | MsG0280010571.01.T01:CDS |
| ACATAGCAGATGCATATTTC+GGG | 0.314837 | 2:+71670373 | MsG0280010571.01.T01:CDS |
| CTAAGCAAGTACTGGCTAAA+AGG | 0.321104 | 2:+71671002 | MsG0280010571.01.T01:CDS |
| ATACTTTCTGGTGATAATAA+TGG | 0.334662 | 2:+71670687 | MsG0280010571.01.T01:CDS |
| TTGGATAGGGCTTTGCTTGA+AGG | 0.344074 | 2:-71669568 | None:intergenic |
| TTGCTTAAGGAAATCCCATT+TGG | 0.360210 | 2:-71670774 | None:intergenic |
| CGGTAAACTTCTGCAAATAT+TGG | 0.362581 | 2:+71671268 | MsG0280010571.01.T01:CDS |
| TGGGATAGGTAGCTTGAGTA+TGG | 0.373674 | 2:-71671177 | None:intergenic |
| ATTCAATACCTGCTACATAT+TGG | 0.377715 | 2:+71671206 | MsG0280010571.01.T01:CDS |
| CAACGAAAATGGTGACCTTT+GGG | 0.392894 | 2:+71671088 | MsG0280010571.01.T01:CDS |
| TTCGTTGACTCTCACATTGT+CGG | 0.397838 | 2:-71671072 | None:intergenic |
| CACAAGAACAGTGGTTTCCT+TGG | 0.403080 | 2:-71670736 | None:intergenic |
| CTAAGATTTGACAAGAAAAC+TGG | 0.404603 | 2:+71670342 | MsG0280010571.01.T01:CDS |
| AGAAATGTTCAATTTCCAAA+TGG | 0.409686 | 2:+71670759 | MsG0280010571.01.T01:CDS |
| TCAACGAAAATGGTGACCTT+TGG | 0.415508 | 2:+71671087 | MsG0280010571.01.T01:CDS |
| ATCATGGTCTGAAGTTCCTC+TGG | 0.416576 | 2:+71669629 | MsG0280010571.01.T01:CDS |
| GGGTGGCTATCCATTGTAGA+AGG | 0.420650 | 2:+71671108 | MsG0280010571.01.T01:CDS |
| TGCTCTCCGGTCCCTGCACT+TGG | 0.422805 | 2:-71669701 | None:intergenic |
| TGGATAGGGCTTTGCTTGAA+GGG | 0.424691 | 2:-71669567 | None:intergenic |
| AGTGGTGTTGCAGATGCCTT+TGG | 0.424846 | 2:-71670276 | None:intergenic |
| GAAGGAACCATCCTTGCTTA+AGG | 0.426528 | 2:-71670787 | None:intergenic |
| AGTGAGTGAAGTGGAGGAGA+AGG | 0.431067 | 2:+71671319 | MsG0280010571.01.T01:CDS |
| CCATCAGCAACACCGGTGTA+AGG | 0.434665 | 2:-71669750 | None:intergenic |
| TTGCATATACCTCGCCCAAC+AGG | 0.446476 | 2:+71669811 | MsG0280010571.01.T01:CDS |
| AGACAACTTGTTGAAGAAAT+CGG | 0.446631 | 2:+71669662 | MsG0280010571.01.T01:CDS |
| GAAATGTTCAATTTCCAAAT+GGG | 0.451526 | 2:+71670760 | MsG0280010571.01.T01:CDS |
| AATGTGAGAGTCAACGAAAA+TGG | 0.457761 | 2:+71671077 | MsG0280010571.01.T01:CDS |
| CTGATGAAGGTGGGGCCTCA+AGG | 0.459786 | 2:+71670396 | MsG0280010571.01.T01:CDS |
| GCAGGTATTGAATCCTTGTT+GGG | 0.464036 | 2:-71671196 | None:intergenic |
| TCTGTAGGCTAAGCAAGTAC+TGG | 0.465199 | 2:+71670994 | MsG0280010571.01.T01:intron |
| ATTTGCAGAAGTTTACCGTC+AGG | 0.467604 | 2:-71671263 | None:intergenic |
| GAAGAGAAGCGTGACCTGTT+GGG | 0.474973 | 2:-71669825 | None:intergenic |
| ACAAAATTAACTTGCCATCA+TGG | 0.475567 | 2:+71669613 | MsG0280010571.01.T01:CDS |
| CCTCAAAATCAGGGAAGCCT+TGG | 0.484602 | 2:-71669587 | None:intergenic |
| TATTGAATCCTTGTTGGGAT+AGG | 0.492693 | 2:-71671191 | None:intergenic |
| TGTACATATACCTTCTACAA+TGG | 0.493786 | 2:-71671118 | None:intergenic |
| TGCATATTTCGGGCTGATGA+AGG | 0.499203 | 2:+71670383 | MsG0280010571.01.T01:CDS |
| AGCATTGCCTTCGATTATCA+TGG | 0.499411 | 2:+71669720 | MsG0280010571.01.T01:CDS |
| TAATCGAAGGCAATGCTCTC+CGG | 0.499769 | 2:-71669714 | None:intergenic |
| AAGAACAGTGGTTTCCTTGG+TGG | 0.500187 | 2:-71670733 | None:intergenic |
| ACGTCATTAGTAAATCTAAA+CGG | 0.500664 | 2:-71670459 | None:intergenic |
| AGTGAAGTGGAGGAGAAGGA+TGG | 0.504056 | 2:+71671323 | MsG0280010571.01.T01:CDS |
| CGCAACATCGCTTGCAACTG+AGG | 0.505541 | 2:+71670425 | MsG0280010571.01.T01:CDS |
| GGGATTTCCTTAAGCAAGGA+TGG | 0.509307 | 2:+71670780 | MsG0280010571.01.T01:CDS |
| AGCAGGTATTGAATCCTTGT+TGG | 0.510712 | 2:-71671197 | None:intergenic |
| AAATCAGGGAAGCCTTGGAT+AGG | 0.512244 | 2:-71669582 | None:intergenic |
| GCCTTCGATTATCATGGCCG+TGG | 0.516919 | 2:+71669726 | MsG0280010571.01.T01:CDS |
| GAAATCTTAGCAATCCTACC+TGG | 0.521950 | 2:+71671044 | MsG0280010571.01.T01:CDS |
| TGGCTAAAAGGAGATAAAGC+TGG | 0.528508 | 2:+71671014 | MsG0280010571.01.T01:CDS |
| CTCTTGTTTGCTCTCTACTG+TGG | 0.530078 | 2:+71669537 | MsG0280010571.01.T01:CDS |
| GGATAGGGCTTTGCTTGAAG+GGG | 0.530593 | 2:-71669566 | None:intergenic |
| AAATGGGATTTCCTTAAGCA+AGG | 0.531261 | 2:+71670776 | MsG0280010571.01.T01:CDS |
| TAAAGCAGTGAGTGAAGTGG+AGG | 0.531558 | 2:+71671313 | MsG0280010571.01.T01:CDS |
| GCAAATATTGGAGGACAATG+AGG | 0.532562 | 2:+71671280 | MsG0280010571.01.T01:CDS |
| TTGTCAAATCTTAGTCCCAA+AGG | 0.536364 | 2:-71670333 | None:intergenic |
| CATGTTTGTGAACCAAGTGC+AGG | 0.540963 | 2:+71669689 | MsG0280010571.01.T01:CDS |
| AAATGATAAACCGCAATGAA+AGG | 0.541109 | 2:-71671374 | None:intergenic |
| TAAACTTCTGCAAATATTGG+AGG | 0.544725 | 2:+71671271 | MsG0280010571.01.T01:CDS |
| CTCACATTGTCGGCAAAACC+AGG | 0.545473 | 2:-71671062 | None:intergenic |
| TTTCAGAAGGAGTATTTGGA+AGG | 0.549054 | 2:+71670817 | MsG0280010571.01.T01:CDS |
| ACACCGGTGTTGCTGATGGA+AGG | 0.549231 | 2:+71669754 | MsG0280010571.01.T01:CDS |
| TATTTCGGGCTGATGAAGGT+GGG | 0.549565 | 2:+71670387 | MsG0280010571.01.T01:CDS |
| TGTTAAAGCAGTGAGTGAAG+TGG | 0.551000 | 2:+71671310 | MsG0280010571.01.T01:CDS |
| AATCAGGGAAGCCTTGGATA+GGG | 0.552957 | 2:-71669581 | None:intergenic |
| AAGGATGGTAAACTCTGGAT+AGG | 0.555622 | 2:+71671338 | MsG0280010571.01.T01:CDS |
| TCCTTCTGAAAATACAAAGA+AGG | 0.555909 | 2:-71670805 | None:intergenic |
| TGTGAACCAAGTGCAGGGAC+CGG | 0.556419 | 2:+71669695 | MsG0280010571.01.T01:CDS |
| CAATACCTGCTACATATTGG+AGG | 0.561063 | 2:+71671209 | MsG0280010571.01.T01:CDS |
| TTCTGGTGATAATAATGGAA+GGG | 0.568249 | 2:+71670692 | MsG0280010571.01.T01:CDS |
| GAAGCGTGACCTGTTGGGCG+AGG | 0.570009 | 2:-71669820 | None:intergenic |
| TTTGCAGAAGTTTACCGTCA+GGG | 0.572710 | 2:-71671262 | None:intergenic |
| CCTTACACCGGTGTTGCTGA+TGG | 0.584330 | 2:+71669750 | MsG0280010571.01.T01:CDS |
| CATGGCCGTGGCCCTTACAC+CGG | 0.584344 | 2:+71669738 | MsG0280010571.01.T01:CDS |
| GACGTAGACGTTGATGCAGA+AGG | 0.584580 | 2:+71670477 | MsG0280010571.01.T01:CDS |
| CATCAGCAACACCGGTGTAA+GGG | 0.586543 | 2:-71669749 | None:intergenic |
| TTTCTGGTGATAATAATGGA+AGG | 0.588715 | 2:+71670691 | MsG0280010571.01.T01:CDS |
| CAAATATTGGAGGACAATGA+GGG | 0.596205 | 2:+71671281 | MsG0280010571.01.T01:CDS |
| GAAATACAATTCTGCCACCA+AGG | 0.601190 | 2:+71670719 | MsG0280010571.01.T01:CDS |
| AATCCTTCCATCAGCAACAC+CGG | 0.602555 | 2:-71669757 | None:intergenic |
| ATTTCGGGCTGATGAAGGTG+GGG | 0.604505 | 2:+71670388 | MsG0280010571.01.T01:CDS |
| AGGAGAAGGATGGTAAACTC+TGG | 0.608335 | 2:+71671333 | MsG0280010571.01.T01:CDS |
| CAACACCGGTGTAAGGGCCA+CGG | 0.609330 | 2:-71669743 | None:intergenic |
| GTCGTTAAGTATAGCCCTGA+CGG | 0.613243 | 2:+71671248 | MsG0280010571.01.T01:CDS |
| GCCACGGCCATGATAATCGA+AGG | 0.615291 | 2:-71669727 | None:intergenic |
| TCAGTGTCAACATAACTAAG+TGG | 0.615561 | 2:-71670294 | None:intergenic |
| ACGTAGACGTTGATGCAGAA+GGG | 0.617394 | 2:+71670478 | MsG0280010571.01.T01:CDS |
| TTGTCTTTGTCATGATCCAG+AGG | 0.617900 | 2:-71669645 | None:intergenic |
| TTGACACTGAGGACATCTGC+GGG | 0.621256 | 2:+71670307 | MsG0280010571.01.T01:CDS |
| ATATTTCGGGCTGATGAAGG+TGG | 0.623437 | 2:+71670386 | MsG0280010571.01.T01:CDS |
| ATGAAGGTGGGGCCTCAAGG+TGG | 0.624685 | 2:+71670399 | MsG0280010571.01.T01:CDS |
| AACATTTCTCACAAGAACAG+TGG | 0.629432 | 2:-71670745 | None:intergenic |
| TCGCTTGCAACTGAGGCAGA+AGG | 0.629466 | 2:+71670432 | MsG0280010571.01.T01:CDS |
| GTCAGAACTATGCAATCCAA+AGG | 0.629677 | 2:+71670260 | MsG0280010571.01.T01:CDS |
| TTCAAGCAAAGCCCTATCCA+AGG | 0.630560 | 2:+71669570 | MsG0280010571.01.T01:CDS |
| GCAGCACTAAATATCAGAGA+AGG | 0.631433 | 2:+71670520 | MsG0280010571.01.T01:CDS |
| ATGTTTGTGAACCAAGTGCA+GGG | 0.633422 | 2:+71669690 | MsG0280010571.01.T01:CDS |
| AGAGGAACTTCAGACCATGA+TGG | 0.637623 | 2:-71669627 | None:intergenic |
| TACAATGGATAGCCACCCAA+AGG | 0.641529 | 2:-71671103 | None:intergenic |
| CGAAAATGGTGACCTTTGGG+TGG | 0.646638 | 2:+71671091 | MsG0280010571.01.T01:CDS |
| GTTGACACTGAGGACATCTG+CGG | 0.649702 | 2:+71670306 | MsG0280010571.01.T01:CDS |
| CATTGTCGGCAAAACCAGGT+AGG | 0.653789 | 2:-71671058 | None:intergenic |
| TCAAGCTACCTATCCCAACA+AGG | 0.673835 | 2:+71671183 | MsG0280010571.01.T01:CDS |
| TCATACATGACGCTAACTCG+AGG | 0.676086 | 2:+71669498 | None:intergenic |
| GTCAGGGCTATACTTAACGA+CGG | 0.676277 | 2:-71671246 | None:intergenic |
| ACACTGAGGACATCTGCGGG+AGG | 0.694284 | 2:+71670310 | MsG0280010571.01.T01:CDS |
| ACTTAGTTATGTTGACACTG+AGG | 0.721000 | 2:+71670296 | MsG0280010571.01.T01:CDS |
| GATGTTGCGAAACCACCTTG+AGG | 0.764503 | 2:-71670411 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAATGTTGATAATTTCAA+AGG | - | Chr2:71670579-71670598 | None:intergenic | 15.0% |
| !! | TATAGTTTCTAACTTAATGT+TGG | + | Chr2:71670021-71670040 | MsG0280010571.01.T01:intron | 20.0% |
| !!! | TTTAAGTTGGAAAAGTTATA+AGG | - | Chr2:71669854-71669873 | None:intergenic | 20.0% |
| ! | ACGTCATTAGTAAATCTAAA+CGG | - | Chr2:71670462-71670481 | None:intergenic | 25.0% |
| ! | AGAAATAGCAAAACAATGTT+TGG | + | Chr2:71670605-71670624 | MsG0280010571.01.T01:intron | 25.0% |
| ! | AGAAATGTTCAATTTCCAAA+TGG | + | Chr2:71670759-71670778 | MsG0280010571.01.T01:CDS | 25.0% |
| ! | ATCGTTATTAGACGAAAAAA+GGG | - | Chr2:71670863-71670882 | None:intergenic | 25.0% |
| ! | GAAATGTTCAATTTCCAAAT+GGG | + | Chr2:71670760-71670779 | MsG0280010571.01.T01:CDS | 25.0% |
| ! | TAAGATGAATGGATAATTTG+TGG | + | Chr2:71670179-71670198 | MsG0280010571.01.T01:intron | 25.0% |
| ! | TATCGTTATTAGACGAAAAA+AGG | - | Chr2:71670864-71670883 | None:intergenic | 25.0% |
| !! | AAGACTTTTCTTAAGATGAA+TGG | + | Chr2:71670168-71670187 | MsG0280010571.01.T01:intron | 25.0% |
| !! | ATACTTTCTGGTGATAATAA+TGG | + | Chr2:71670687-71670706 | MsG0280010571.01.T01:CDS | 25.0% |
| AAATGATAAACCGCAATGAA+AGG | - | Chr2:71671377-71671396 | None:intergenic | 30.0% | |
| ATAGTGCAAAACCTATTAGT+AGG | + | Chr2:71669975-71669994 | MsG0280010571.01.T01:intron | 30.0% | |
| ATTCAATACCTGCTACATAT+TGG | + | Chr2:71671206-71671225 | MsG0280010571.01.T01:CDS | 30.0% | |
| CTAAACATATTGTGTCAGAA+TGG | - | Chr2:71670933-71670952 | None:intergenic | 30.0% | |
| CTAAGATTTGACAAGAAAAC+TGG | + | Chr2:71670342-71670361 | MsG0280010571.01.T01:CDS | 30.0% | |
| CTTGTACTAAGCATTTAAGT+TGG | - | Chr2:71669867-71669886 | None:intergenic | 30.0% | |
| TAAACTTCTGCAAATATTGG+AGG | + | Chr2:71671271-71671290 | MsG0280010571.01.T01:CDS | 30.0% | |
| TACATAGCAGATGCATATTT+CGG | + | Chr2:71670372-71670391 | MsG0280010571.01.T01:CDS | 30.0% | |
| TAGTGCAAAACCTATTAGTA+GGG | + | Chr2:71669976-71669995 | MsG0280010571.01.T01:intron | 30.0% | |
| TGTACATATACCTTCTACAA+TGG | - | Chr2:71671121-71671140 | None:intergenic | 30.0% | |
| TTTAATAGTATGAGCGTATG+AGG | - | Chr2:71670072-71670091 | None:intergenic | 30.0% | |
| ! | AGTATGGCTTTTCTAATCTT+TGG | - | Chr2:71671164-71671183 | None:intergenic | 30.0% |
| ! | GTATGGCTTTTCTAATCTTT+GGG | - | Chr2:71671163-71671182 | None:intergenic | 30.0% |
| ! | TCCTTCTGAAAATACAAAGA+AGG | - | Chr2:71670808-71670827 | None:intergenic | 30.0% |
| ! | TCCTTCTTTGTATTTTCAGA+AGG | + | Chr2:71670804-71670823 | MsG0280010571.01.T01:CDS | 30.0% |
| ! | TTCATTCAGTTGATACTTTC+TGG | + | Chr2:71670675-71670694 | MsG0280010571.01.T01:CDS | 30.0% |
| ! | TTCTGGTGATAATAATGGAA+GGG | + | Chr2:71670692-71670711 | MsG0280010571.01.T01:CDS | 30.0% |
| ! | TTTCTGGTGATAATAATGGA+AGG | + | Chr2:71670691-71670710 | MsG0280010571.01.T01:CDS | 30.0% |
| !! | ACAAAATTAACTTGCCATCA+TGG | + | Chr2:71669613-71669632 | MsG0280010571.01.T01:CDS | 30.0% |
| !! | AGACAACTTGTTGAAGAAAT+CGG | + | Chr2:71669662-71669681 | MsG0280010571.01.T01:CDS | 30.0% |
| !!! | GTATTTTCAGAAGGAGTATT+TGG | + | Chr2:71670813-71670832 | MsG0280010571.01.T01:CDS | 30.0% |
| !!! | TCTGTAACTTTTTCACAGAT+CGG | - | Chr2:71670112-71670131 | None:intergenic | 30.0% |
| AAATGGGATTTCCTTAAGCA+AGG | + | Chr2:71670776-71670795 | MsG0280010571.01.T01:CDS | 35.0% | |
| AACATTTCTCACAAGAACAG+TGG | - | Chr2:71670748-71670767 | None:intergenic | 35.0% | |
| AATGTGAGAGTCAACGAAAA+TGG | + | Chr2:71671077-71671096 | MsG0280010571.01.T01:CDS | 35.0% | |
| ACATAGCAGATGCATATTTC+GGG | + | Chr2:71670373-71670392 | MsG0280010571.01.T01:CDS | 35.0% | |
| ACTTAGTTATGTTGACACTG+AGG | + | Chr2:71670296-71670315 | MsG0280010571.01.T01:CDS | 35.0% | |
| AGTGCAAAACCTATTAGTAG+GGG | + | Chr2:71669977-71669996 | MsG0280010571.01.T01:intron | 35.0% | |
| ATAGCAAAACAATGTTTGGC+TGG | + | Chr2:71670609-71670628 | MsG0280010571.01.T01:intron | 35.0% | |
| CAAATATTGGAGGACAATGA+GGG | + | Chr2:71671281-71671300 | MsG0280010571.01.T01:CDS | 35.0% | |
| CACATATATCCCCTACTAAT+AGG | - | Chr2:71669989-71670008 | None:intergenic | 35.0% | |
| CGGTAAACTTCTGCAAATAT+TGG | + | Chr2:71671268-71671287 | MsG0280010571.01.T01:CDS | 35.0% | |
| CTATTAGTAGGGGATATATG+TGG | + | Chr2:71669987-71670006 | MsG0280010571.01.T01:intron | 35.0% | |
| GTCAGAATGGCAAAATATGT+TGG | - | Chr2:71670920-71670939 | None:intergenic | 35.0% | |
| TATTGAATCCTTGTTGGGAT+AGG | - | Chr2:71671194-71671213 | None:intergenic | 35.0% | |
| TCAGTGTCAACATAACTAAG+TGG | - | Chr2:71670297-71670316 | None:intergenic | 35.0% | |
| TGCTTAGCCTACAGAAAAAA+CGG | - | Chr2:71670989-71671008 | None:intergenic | 35.0% | |
| TTGCTTAAGGAAATCCCATT+TGG | - | Chr2:71670777-71670796 | None:intergenic | 35.0% | |
| TTGTCAAATCTTAGTCCCAA+AGG | - | Chr2:71670336-71670355 | None:intergenic | 35.0% | |
| ! | ATTTTGTGCACCTCAAAATC+AGG | - | Chr2:71669600-71669619 | None:intergenic | 35.0% |
| ! | TTTTGTGCACCTCAAAATCA+GGG | - | Chr2:71669599-71669618 | None:intergenic | 35.0% |
| !! | CTGATGGAAGGATTTTGTTT+TGG | + | Chr2:71669766-71669785 | MsG0280010571.01.T01:CDS | 35.0% |
| !! | TTTCAGAAGGAGTATTTGGA+AGG | + | Chr2:71670817-71670836 | MsG0280010571.01.T01:CDS | 35.0% |
| !!! | ATCAAGTCCGTTTTTTCTGT+AGG | + | Chr2:71670979-71670998 | MsG0280010571.01.T01:intron | 35.0% |
| !!! | GGAAGGATTTTGTTTTGGAA+TGG | + | Chr2:71669771-71669790 | MsG0280010571.01.T01:CDS | 35.0% |
| !!! | TGTTTTGATGCCTTTCATTG+CGG | + | Chr2:71671364-71671383 | MsG0280010571.01.T01:CDS | 35.0% |
| AAGGATGGTAAACTCTGGAT+AGG | + | Chr2:71671338-71671357 | MsG0280010571.01.T01:CDS | 40.0% | |
| AGCATTGCCTTCGATTATCA+TGG | + | Chr2:71669720-71669739 | MsG0280010571.01.T01:CDS | 40.0% | |
| ATGTTTGTGAACCAAGTGCA+GGG | + | Chr2:71669690-71669709 | MsG0280010571.01.T01:CDS | 40.0% | |
| ATTCTGCTACATTGCATCTG+AGG | + | Chr2:71670145-71670164 | MsG0280010571.01.T01:intron | 40.0% | |
| ATTTGCAGAAGTTTACCGTC+AGG | - | Chr2:71671266-71671285 | None:intergenic | 40.0% | |
| CAACGAAAATGGTGACCTTT+GGG | + | Chr2:71671088-71671107 | MsG0280010571.01.T01:CDS | 40.0% | |
| CAATACCTGCTACATATTGG+AGG | + | Chr2:71671209-71671228 | MsG0280010571.01.T01:CDS | 40.0% | |
| CTGACCTGTGTTCAGAAATA+AGG | - | Chr2:71670245-71670264 | None:intergenic | 40.0% | |
| GAAATACAATTCTGCCACCA+AGG | + | Chr2:71670719-71670738 | MsG0280010571.01.T01:CDS | 40.0% | |
| GAAATCTTAGCAATCCTACC+TGG | + | Chr2:71671044-71671063 | MsG0280010571.01.T01:CDS | 40.0% | |
| GCAAATATTGGAGGACAATG+AGG | + | Chr2:71671280-71671299 | MsG0280010571.01.T01:CDS | 40.0% | |
| GCAGCACTAAATATCAGAGA+AGG | + | Chr2:71670520-71670539 | MsG0280010571.01.T01:CDS | 40.0% | |
| GTACAATGACATCAGAAGTC+AGG | - | Chr2:71670645-71670664 | None:intergenic | 40.0% | |
| GTCAGAACTATGCAATCCAA+AGG | + | Chr2:71670260-71670279 | MsG0280010571.01.T01:CDS | 40.0% | |
| TCAACGAAAATGGTGACCTT+TGG | + | Chr2:71671087-71671106 | MsG0280010571.01.T01:CDS | 40.0% | |
| TCTGATGTCATTGTACAAGC+AGG | + | Chr2:71670649-71670668 | MsG0280010571.01.T01:intron | 40.0% | |
| TGGCTAAAAGGAGATAAAGC+TGG | + | Chr2:71671014-71671033 | MsG0280010571.01.T01:CDS | 40.0% | |
| TGTTAAAGCAGTGAGTGAAG+TGG | + | Chr2:71671310-71671329 | MsG0280010571.01.T01:CDS | 40.0% | |
| TTGTCTTTGTCATGATCCAG+AGG | - | Chr2:71669648-71669667 | None:intergenic | 40.0% | |
| TTTGCAGAAGTTTACCGTCA+GGG | - | Chr2:71671265-71671284 | None:intergenic | 40.0% | |
| ! | TTTTGCCTCCAATATGTAGC+AGG | - | Chr2:71671217-71671236 | None:intergenic | 40.0% |
| !! | AGCAGGTATTGAATCCTTGT+TGG | - | Chr2:71671200-71671219 | None:intergenic | 40.0% |
| !! | CTAAGCAAGTACTGGCTAAA+AGG | + | Chr2:71671002-71671021 | MsG0280010571.01.T01:CDS | 40.0% |
| !! | GCAGGTATTGAATCCTTGTT+GGG | - | Chr2:71671199-71671218 | None:intergenic | 40.0% |
| !! | TTCGTTGACTCTCACATTGT+CGG | - | Chr2:71671075-71671094 | None:intergenic | 40.0% |
| !!! | CAATGTTTGGCTGGTTTTGT+TGG | + | Chr2:71670618-71670637 | MsG0280010571.01.T01:intron | 40.0% |
| !!! | TGTTTTGGAATGGACTGTCT+TGG | + | Chr2:71669781-71669800 | MsG0280010571.01.T01:CDS | 40.0% |
| AAATCAGGGAAGCCTTGGAT+AGG | - | Chr2:71669585-71669604 | None:intergenic | 45.0% | |
| AAGAACAGTGGTTTCCTTGG+TGG | - | Chr2:71670736-71670755 | None:intergenic | 45.0% | |
| AATCAGGGAAGCCTTGGATA+GGG | - | Chr2:71669584-71669603 | None:intergenic | 45.0% | |
| AATCCTTCCATCAGCAACAC+CGG | - | Chr2:71669760-71669779 | None:intergenic | 45.0% | |
| ACGTAGACGTTGATGCAGAA+GGG | + | Chr2:71670478-71670497 | MsG0280010571.01.T01:CDS | 45.0% | |
| AGAGGAACTTCAGACCATGA+TGG | - | Chr2:71669630-71669649 | None:intergenic | 45.0% | |
| AGGAGAAGGATGGTAAACTC+TGG | + | Chr2:71671333-71671352 | MsG0280010571.01.T01:CDS | 45.0% | |
| ATATTTCGGGCTGATGAAGG+TGG | + | Chr2:71670386-71670405 | MsG0280010571.01.T01:CDS | 45.0% | |
| CACAAGAACAGTGGTTTCCT+TGG | - | Chr2:71670739-71670758 | None:intergenic | 45.0% | |
| CATGTTTGTGAACCAAGTGC+AGG | + | Chr2:71669689-71669708 | MsG0280010571.01.T01:CDS | 45.0% | |
| GAAGGAACCATCCTTGCTTA+AGG | - | Chr2:71670790-71670809 | None:intergenic | 45.0% | |
| GGGATTTCCTTAAGCAAGGA+TGG | + | Chr2:71670780-71670799 | MsG0280010571.01.T01:CDS | 45.0% | |
| GTCAGGGCTATACTTAACGA+CGG | - | Chr2:71671249-71671268 | None:intergenic | 45.0% | |
| GTCGTTAAGTATAGCCCTGA+CGG | + | Chr2:71671248-71671267 | MsG0280010571.01.T01:CDS | 45.0% | |
| TAAAGCAGTGAGTGAAGTGG+AGG | + | Chr2:71671313-71671332 | MsG0280010571.01.T01:CDS | 45.0% | |
| TAATCGAAGGCAATGCTCTC+CGG | - | Chr2:71669717-71669736 | None:intergenic | 45.0% | |
| TACAATGGATAGCCACCCAA+AGG | - | Chr2:71671106-71671125 | None:intergenic | 45.0% | |
| TATTTCGGGCTGATGAAGGT+GGG | + | Chr2:71670387-71670406 | MsG0280010571.01.T01:CDS | 45.0% | |
| TCAAGCTACCTATCCCAACA+AGG | + | Chr2:71671183-71671202 | MsG0280010571.01.T01:CDS | 45.0% | |
| TCTGTAGGCTAAGCAAGTAC+TGG | + | Chr2:71670994-71671013 | MsG0280010571.01.T01:intron | 45.0% | |
| TGCATATTTCGGGCTGATGA+AGG | + | Chr2:71670383-71670402 | MsG0280010571.01.T01:CDS | 45.0% | |
| TGGATAGGGCTTTGCTTGAA+GGG | - | Chr2:71669570-71669589 | None:intergenic | 45.0% | |
| TGGGATAGGTAGCTTGAGTA+TGG | - | Chr2:71671180-71671199 | None:intergenic | 45.0% | |
| TTCAAGCAAAGCCCTATCCA+AGG | + | Chr2:71669570-71669589 | MsG0280010571.01.T01:CDS | 45.0% | |
| TTGGATAGGGCTTTGCTTGA+AGG | - | Chr2:71669571-71669590 | None:intergenic | 45.0% | |
| ! | ATCATGGTCTGAAGTTCCTC+TGG | + | Chr2:71669629-71669648 | MsG0280010571.01.T01:CDS | 45.0% |
| ! | CTCTTGTTTGCTCTCTACTG+TGG | + | Chr2:71669537-71669556 | MsG0280010571.01.T01:CDS | 45.0% |
| !! | CGCACCTTATTTCTGAACAC+AGG | + | Chr2:71670238-71670257 | MsG0280010571.01.T01:intron | 45.0% |
| AGTGAAGTGGAGGAGAAGGA+TGG | + | Chr2:71671323-71671342 | MsG0280010571.01.T01:CDS | 50.0% | |
| AGTGAGTGAAGTGGAGGAGA+AGG | + | Chr2:71671319-71671338 | MsG0280010571.01.T01:CDS | 50.0% | |
| ATTTCGGGCTGATGAAGGTG+GGG | + | Chr2:71670388-71670407 | MsG0280010571.01.T01:CDS | 50.0% | |
| CATCAGCAACACCGGTGTAA+GGG | - | Chr2:71669752-71669771 | None:intergenic | 50.0% | |
| CATTGTCGGCAAAACCAGGT+AGG | - | Chr2:71671061-71671080 | None:intergenic | 50.0% | |
| CCTCAAAATCAGGGAAGCCT+TGG | - | Chr2:71669590-71669609 | None:intergenic | 50.0% | |
| CGAAAATGGTGACCTTTGGG+TGG | + | Chr2:71671091-71671110 | MsG0280010571.01.T01:CDS | 50.0% | |
| CTCACATTGTCGGCAAAACC+AGG | - | Chr2:71671065-71671084 | None:intergenic | 50.0% | |
| GACGTAGACGTTGATGCAGA+AGG | + | Chr2:71670477-71670496 | MsG0280010571.01.T01:CDS | 50.0% | |
| GATGTTGCGAAACCACCTTG+AGG | - | Chr2:71670414-71670433 | None:intergenic | 50.0% | |
| GGATAGGGCTTTGCTTGAAG+GGG | - | Chr2:71669569-71669588 | None:intergenic | 50.0% | |
| GGGTGGCTATCCATTGTAGA+AGG | + | Chr2:71671108-71671127 | MsG0280010571.01.T01:CDS | 50.0% | |
| GTTGACACTGAGGACATCTG+CGG | + | Chr2:71670306-71670325 | MsG0280010571.01.T01:CDS | 50.0% | |
| TTGACACTGAGGACATCTGC+GGG | + | Chr2:71670307-71670326 | MsG0280010571.01.T01:CDS | 50.0% | |
| TTGCATATACCTCGCCCAAC+AGG | + | Chr2:71669811-71669830 | MsG0280010571.01.T01:CDS | 50.0% | |
| ! | AGAAGAGAAGCGTGACCTGT+TGG | - | Chr2:71669829-71669848 | None:intergenic | 50.0% |
| ! | CCAAGGCTTCCCTGATTTTG+AGG | + | Chr2:71669587-71669606 | MsG0280010571.01.T01:CDS | 50.0% |
| ! | GAAGAGAAGCGTGACCTGTT+GGG | - | Chr2:71669828-71669847 | None:intergenic | 50.0% |
| !! | AGTGGTGTTGCAGATGCCTT+TGG | - | Chr2:71670279-71670298 | None:intergenic | 50.0% |
| CCATCAGCAACACCGGTGTA+AGG | - | Chr2:71669753-71669772 | None:intergenic | 55.0% | |
| CGCAACATCGCTTGCAACTG+AGG | + | Chr2:71670425-71670444 | MsG0280010571.01.T01:CDS | 55.0% | |
| GCCACGGCCATGATAATCGA+AGG | - | Chr2:71669730-71669749 | None:intergenic | 55.0% | |
| GCCTTCGATTATCATGGCCG+TGG | + | Chr2:71669726-71669745 | MsG0280010571.01.T01:CDS | 55.0% | |
| TCGCTTGCAACTGAGGCAGA+AGG | + | Chr2:71670432-71670451 | MsG0280010571.01.T01:CDS | 55.0% | |
| TGTGAACCAAGTGCAGGGAC+CGG | + | Chr2:71669695-71669714 | MsG0280010571.01.T01:CDS | 55.0% | |
| !! | ACACCGGTGTTGCTGATGGA+AGG | + | Chr2:71669754-71669773 | MsG0280010571.01.T01:CDS | 55.0% |
| !! | CCTTACACCGGTGTTGCTGA+TGG | + | Chr2:71669750-71669769 | MsG0280010571.01.T01:CDS | 55.0% |
| ACACTGAGGACATCTGCGGG+AGG | + | Chr2:71670310-71670329 | MsG0280010571.01.T01:CDS | 60.0% | |
| ATGAAGGTGGGGCCTCAAGG+TGG | + | Chr2:71670399-71670418 | MsG0280010571.01.T01:CDS | 60.0% | |
| CAACACCGGTGTAAGGGCCA+CGG | - | Chr2:71669746-71669765 | None:intergenic | 60.0% | |
| CTGATGAAGGTGGGGCCTCA+AGG | + | Chr2:71670396-71670415 | MsG0280010571.01.T01:CDS | 60.0% | |
| GACATCTGCGGGAGGCCTTT+GGG | + | Chr2:71670318-71670337 | MsG0280010571.01.T01:CDS | 60.0% | |
| CATGGCCGTGGCCCTTACAC+CGG | + | Chr2:71669738-71669757 | MsG0280010571.01.T01:CDS | 65.0% | |
| GAAGCGTGACCTGTTGGGCG+AGG | - | Chr2:71669823-71669842 | None:intergenic | 65.0% | |
| GGACATCTGCGGGAGGCCTT+TGG | + | Chr2:71670317-71670336 | MsG0280010571.01.T01:CDS | 65.0% | |
| TGCTCTCCGGTCCCTGCACT+TGG | - | Chr2:71669704-71669723 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 71669504 | 71671403 | 71669504 | ID=MsG0280010571.01;Name=MsG0280010571.01 |
| Chr2 | mRNA | 71669504 | 71671403 | 71669504 | ID=MsG0280010571.01.T01;Parent=MsG0280010571.01;Name=MsG0280010571.01.T01;_AED=0.24;_eAED=0.24;_QI=0|0|0|1|1|1|4|0|393 |
| Chr2 | exon | 71669504 | 71669832 | 71669504 | ID=MsG0280010571.01.T01:exon:6860;Parent=MsG0280010571.01.T01 |
| Chr2 | exon | 71670260 | 71670541 | 71670260 | ID=MsG0280010571.01.T01:exon:6861;Parent=MsG0280010571.01.T01 |
| Chr2 | exon | 71670671 | 71670838 | 71670671 | ID=MsG0280010571.01.T01:exon:6862;Parent=MsG0280010571.01.T01 |
| Chr2 | exon | 71671001 | 71671403 | 71671001 | ID=MsG0280010571.01.T01:exon:6863;Parent=MsG0280010571.01.T01 |
| Chr2 | CDS | 71669504 | 71669832 | 71669504 | ID=MsG0280010571.01.T01:cds;Parent=MsG0280010571.01.T01 |
| Chr2 | CDS | 71670260 | 71670541 | 71670260 | ID=MsG0280010571.01.T01:cds;Parent=MsG0280010571.01.T01 |
| Chr2 | CDS | 71670671 | 71670838 | 71670671 | ID=MsG0280010571.01.T01:cds;Parent=MsG0280010571.01.T01 |
| Chr2 | CDS | 71671001 | 71671403 | 71671001 | ID=MsG0280010571.01.T01:cds;Parent=MsG0280010571.01.T01 |
| Gene Sequence |
| Protein sequence |
