AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010696.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010696.01.T01 MTR_2g090155 86.322 329 12 1 1 296 1 329 0.0 562
MsG0280010696.01.T01 MTR_2g090155 85.030 334 12 1 1 296 1 334 0.0 561
MsG0280010696.01.T01 MTR_2g090155 84.543 317 11 1 1 279 1 317 0.0 527
MsG0280010696.01.T01 MTR_2g080040 60.246 244 59 1 68 273 122 365 1.45e-101 302
MsG0280010696.01.T01 MTR_2g090170 44.737 114 17 2 139 251 1 69 1.15e-17 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010696.01.T01 AT4G03410 56.550 313 89 3 5 276 13 319 1.57e-119 348
MsG0280010696.01.T01 AT4G03410 56.958 309 86 3 5 272 13 315 2.56e-119 345
MsG0280010696.01.T01 AT1G52870 50.000 352 88 6 2 267 3 352 1.07e-106 315
MsG0280010696.01.T01 AT1G52870 42.395 309 86 7 2 224 3 305 2.15e-69 218

Find 80 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCATCTACTTTGTGGTTT+TGG 0.095576 2:+73496774 MsG0280010696.01.T01:CDS
CTGTGGAACAAAGGCTTCTT+TGG 0.173165 2:+73497403 MsG0280010696.01.T01:CDS
GTTTGGTGCGAAATTAATTA+AGG 0.189591 2:-73495219 None:intergenic
TCTTAGTTACCTTAGAATTT+TGG 0.194039 2:-73498338 None:intergenic
CACAGGCAGGCTGGAAGCTT+TGG 0.250296 2:+73497346 MsG0280010696.01.T01:intron
TTCGCCCATCTGATTACTTA+TGG 0.254055 2:+73497372 MsG0280010696.01.T01:CDS
GACTCTTTGGATAACATCTA+TGG 0.262207 2:+73496811 MsG0280010696.01.T01:CDS
TTTGTTGCGTTTCGACTCTT+TGG 0.263346 2:+73496798 MsG0280010696.01.T01:CDS
GCTGCAATCGTTCTCGGATT+TGG 0.274226 2:+73495518 MsG0280010696.01.T01:CDS
AAACTGTGTGGTCAGCTATT+TGG 0.279478 2:+73496749 MsG0280010696.01.T01:CDS
TGGACTGTATTGAGCTCATC+TGG 0.292811 2:+73497427 MsG0280010696.01.T01:CDS
GAAAGGAGGTAGGGAGATGT+GGG 0.356126 2:-73495321 None:intergenic
TCACCATTGATCATGATTAC+TGG 0.368079 2:+73495564 MsG0280010696.01.T01:CDS
TGTGGAACAAAGGCTTCTTT+GGG 0.369696 2:+73497404 MsG0280010696.01.T01:CDS
AGAAAGGAGGTAGGGAGATG+TGG 0.370599 2:-73495322 None:intergenic
ATGATCAATGGTGAGCAATT+TGG 0.384077 2:-73495555 None:intergenic
TACACCAAGCCAAAATTCTA+AGG 0.390257 2:+73498329 MsG0280010696.01.T01:CDS
TTTGGTGCGAAATTAATTAA+GGG 0.392774 2:-73495218 None:intergenic
AGTGGAGTTGTCTACTCAAT+TGG 0.403111 2:+73496364 MsG0280010696.01.T01:CDS
TTGGAACACCATCTACTTTG+TGG 0.406483 2:+73496768 MsG0280010696.01.T01:CDS
TGCGAAATTAATTAAGGGAT+TGG 0.407194 2:-73495213 None:intergenic
TGGTCAAAAGCAACTTTAGC+AGG 0.412786 2:-73496727 None:intergenic
ATCAATGCTGCAATCGTTCT+CGG 0.414955 2:+73495512 MsG0280010696.01.T01:CDS
GAGAACGATTGCAGCATTGA+TGG 0.419624 2:-73495510 None:intergenic
AAATAGCTGACCACACAGTT+TGG 0.423298 2:-73496747 None:intergenic
CAGGGACAACCCACCATTGT+TGG 0.429883 2:-73496708 None:intergenic
TTGTCTACTCAATTGGAGAT+TGG 0.442062 2:+73496371 MsG0280010696.01.T01:CDS
TAGGCAATCCAATTGTGTTC+AGG 0.443916 2:-73496295 None:intergenic
TGCAGCATTGATGGCACGTC+CGG 0.446757 2:-73495501 None:intergenic
GAGATGTGGGGCTTTGAGAA+GGG 0.453423 2:-73495308 None:intergenic
TAAGTTTCACGAGGACTCTC+TGG 0.453787 2:-73498398 None:intergenic
ACCCAAAACCACAAAGTAGA+TGG 0.457211 2:-73496776 None:intergenic
AGAGGATCGTGAAGCAGCTT+CGG 0.458460 2:+73495391 MsG0280010696.01.T01:CDS
TTTGGGAAGAGAAAGGAGGT+AGG 0.471467 2:-73495331 None:intergenic
GGATTCTTCTTCGCCTTCCA+AGG 0.473472 2:+73495442 MsG0280010696.01.T01:CDS
TCTTTCTTTAAATTCTCTCA+TGG 0.476604 2:+73495250 MsG0280010696.01.T01:exon
GGTTCTAGTTGAGAATTGAT+TGG 0.477105 2:-73495158 None:intergenic
AAATTAATTAAGGGATTGGT+GGG 0.477447 2:-73495209 None:intergenic
GGAGATGTGGGGCTTTGAGA+AGG 0.481259 2:-73495309 None:intergenic
AATATGTGCCTGAACACAAT+TGG 0.484652 2:+73496287 MsG0280010696.01.T01:CDS
TTTGATTCTACAGAGAAAGA+AGG 0.485854 2:-73495179 None:intergenic
AATTGGAGATTGGATTGCTC+AGG 0.486533 2:+73496381 MsG0280010696.01.T01:CDS
TGCTTTCAGAGATTGCTCAT+AGG 0.490513 2:-73496314 None:intergenic
GAGGAAGTGTCAGAAACAAC+AGG 0.491215 2:+73498300 MsG0280010696.01.T01:CDS
TTAGCTAAAATGGCAATAAG+TGG 0.494093 2:+73496346 MsG0280010696.01.T01:CDS
TTGGGAAGAGAAAGGAGGTA+GGG 0.500264 2:-73495330 None:intergenic
AATATGCTTCACAGGCAGGC+TGG 0.502341 2:+73497337 MsG0280010696.01.T01:intron
GAAATTAATTAAGGGATTGG+TGG 0.516216 2:-73495210 None:intergenic
TACTTATGGTGTGGTTCCTG+TGG 0.517838 2:+73497386 MsG0280010696.01.T01:CDS
GGGGCTTTGAGAAGGGAAGG+AGG 0.527578 2:-73495301 None:intergenic
CAAAGAAGCCTTTGTTCCAC+AGG 0.539036 2:-73497402 None:intergenic
TTGGTTTGGGAAGAGAAAGG+AGG 0.539173 2:-73495335 None:intergenic
TGTGGGGCTTTGAGAAGGGA+AGG 0.541270 2:-73495304 None:intergenic
GGAACAAAGGCTTCTTTGGG+TGG 0.543292 2:+73497407 MsG0280010696.01.T01:CDS
ATAAGTTCTTACAGTGAGCA+TGG 0.545807 2:-73496857 None:intergenic
AGCAACCTCTGTAATACCCT+TGG 0.551215 2:-73495459 None:intergenic
ATGCCAGTAATCATGATCAA+TGG 0.555376 2:-73495567 None:intergenic
CCACACCATAAGTAATCAGA+TGG 0.560187 2:-73497377 None:intergenic
GGACTGTATTGAGCTCATCT+GGG 0.562988 2:+73497428 MsG0280010696.01.T01:CDS
GATTCTTCTTCGCCTTCCAA+GGG 0.565561 2:+73495443 MsG0280010696.01.T01:CDS
CCATCTGATTACTTATGGTG+TGG 0.569311 2:+73497377 MsG0280010696.01.T01:CDS
ACAACCCACCATTGTTGGAA+AGG 0.581867 2:-73496703 None:intergenic
TAAGTTCTTACAGTGAGCAT+GGG 0.590336 2:-73496856 None:intergenic
GGGCTTTGAGAAGGGAAGGA+GGG 0.590501 2:-73495300 None:intergenic
ATCAGAATCCCGAAAATCTG+AGG 0.598586 2:+73498281 MsG0280010696.01.T01:CDS
GGTCAAAAGCAACTTTAGCA+GGG 0.608265 2:-73496726 None:intergenic
TAAGTAATCAGATGGGCGAA+AGG 0.609767 2:-73497369 None:intergenic
CATAATCAGCTCCGTTACAG+AGG 0.615880 2:+73495373 MsG0280010696.01.T01:CDS
TGCTTCACGATCCTCTGTAA+CGG 0.624438 2:-73495384 None:intergenic
AATAGAAGAAGCAACTGACA+TGG 0.637698 2:-73495273 None:intergenic
GCCTTCCAAGGGTATTACAG+AGG 0.638135 2:+73495454 MsG0280010696.01.T01:CDS
ACCTCTGTAATACCCTTGGA+AGG 0.643738 2:-73495455 None:intergenic
GTGTGGTTCCTGTGGAACAA+AGG 0.645875 2:+73497394 MsG0280010696.01.T01:CDS
AAAAGACAGAAAAGTCCTTG+AGG 0.646563 2:+73495421 MsG0280010696.01.T01:CDS
CATTGATGGCACGTCCGGTG+AGG 0.652622 2:-73495496 None:intergenic
GCTGACACTGATATCCTCAC+CGG 0.657443 2:+73495482 MsG0280010696.01.T01:CDS
AAGGCGAAGAAGAATCCTCA+AGG 0.659435 2:-73495436 None:intergenic
AAAGGAGGTAGGGAGATGTG+GGG 0.663791 2:-73495320 None:intergenic
CACACCATAAGTAATCAGAT+GGG 0.679652 2:-73497376 None:intergenic
ATTTCGTGATAAGTTTCACG+AGG 0.681834 2:-73498407 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTGTTTCT+AGG + Chr2:73498244-73498263 MsG0280010696.01.T01:intron 10.0%
!! AATTAAATCTCATTTAAGAA+TGG + Chr2:73497694-73497713 MsG0280010696.01.T01:intron 15.0%
!! ATAAATTAAGAAACATAGTT+TGG - Chr2:73496013-73496032 None:intergenic 15.0%
!! TTAAATGAGATTTAATTACA+AGG - Chr2:73497691-73497710 None:intergenic 15.0%
!!! ACAATTTTTTTTTTTTCAGT+CGG + Chr2:73497511-73497530 MsG0280010696.01.T01:intron 15.0%
!!! TAAGCAAAAATCAATTTTTT+AGG + Chr2:73497860-73497879 MsG0280010696.01.T01:intron 15.0%
!!! TAAGCAAAAATCAATTTTTT+AGG - Chr2:73497879-73497860 None:intergenic 15.0%
!! ATAGAAAAAGAAGCAAAATT+TGG - Chr2:73495615-73495634 None:intergenic 20.0%
!! ATGACAGAAAAATAATACAA+CGG - Chr2:73498367-73498386 None:intergenic 20.0%
!! CATTCATTAAATGATGTATT+TGG + Chr2:73497885-73497904 MsG0280010696.01.T01:intron 20.0%
!! GATAAAAAATCAATTCAAAC+AGG - Chr2:73496896-73496915 None:intergenic 20.0%
!! TGTTACAAAAATAAAGCAAA+AGG + Chr2:73497159-73497178 MsG0280010696.01.T01:intron 20.0%
!!! AAAGAAAGATTTTGTTTGTT+TGG - Chr2:73495239-73495258 None:intergenic 20.0%
!!! AAGAACTTATTTGTTTCTTA+GGG + Chr2:73496870-73496889 MsG0280010696.01.T01:intron 20.0%
!!! TAAGAACTTATTTGTTTCTT+AGG + Chr2:73496869-73496888 MsG0280010696.01.T01:intron 20.0%
!!! TATTTTTCTGTCATGTTTTA+AGG + Chr2:73498373-73498392 MsG0280010696.01.T01:CDS 20.0%
!!! TCAATAATAAGCTAGTTTAT+TGG - Chr2:73495689-73495708 None:intergenic 20.0%
!!! TGAATTGATTTTTTATCTAC+TGG + Chr2:73496899-73496918 MsG0280010696.01.T01:intron 20.0%
!!! TGTATTTTCTGTATTGTAAA+TGG + Chr2:73496203-73496222 MsG0280010696.01.T01:intron 20.0%
! AAAAACCAAACCAGATTAAT+TGG + Chr2:73497597-73497616 MsG0280010696.01.T01:intron 25.0%
! AAAAACCAGACCAATTTAAT+TGG + Chr2:73498151-73498170 MsG0280010696.01.T01:intron 25.0%
! AAAATCCAATTAAATTGGTC+TGG - Chr2:73498159-73498178 None:intergenic 25.0%
! AACGCAAAATCCAATTAAAT+TGG - Chr2:73498164-73498183 None:intergenic 25.0%
! AAGAAGTTGAAAACTGATTA+GGG + Chr2:73496226-73496245 MsG0280010696.01.T01:intron 25.0%
! AATATAGAGAAGAGTAGAAA+AGG + Chr2:73497725-73497744 MsG0280010696.01.T01:intron 25.0%
! AGAAAATTGATGAAAGAAAG+GGG + Chr2:73497182-73497201 MsG0280010696.01.T01:intron 25.0%
! AGTTTGGTATAGATAGAATA+TGG - Chr2:73495997-73496016 None:intergenic 25.0%
! ATGTATTATAGTAAAGCAGA+CGG + Chr2:73496156-73496175 MsG0280010696.01.T01:intron 25.0%
! ATTATTGTACATCTTTGGTT+TGG + Chr2:73496609-73496628 MsG0280010696.01.T01:intron 25.0%
! CAAAATATATAGTGCTATGA+AGG + Chr2:73496480-73496499 MsG0280010696.01.T01:intron 25.0%
! GAGAAAATTGATGAAAGAAA+GGG + Chr2:73497181-73497200 MsG0280010696.01.T01:intron 25.0%
! TAAAGCCAAATTAAGAAACA+CGG - Chr2:73496057-73496076 None:intergenic 25.0%
! TATGAGATACTTATCCAAAA+GGG - Chr2:73497665-73497684 None:intergenic 25.0%
! TCTTTCTTTAAATTCTCTCA+TGG + Chr2:73495250-73495269 MsG0280010696.01.T01:exon 25.0%
! TGCTATAATCTAGTAGAAAA+AGG + Chr2:73495815-73495834 MsG0280010696.01.T01:intron 25.0%
!! AAATTAATTAAGGGATTGGT+GGG - Chr2:73495212-73495231 None:intergenic 25.0%
!! AAATTATGAAGTGCTTATGT+TGG + Chr2:73496428-73496447 MsG0280010696.01.T01:intron 25.0%
!! AGTCAAACTATTTTCATACA+AGG - Chr2:73496974-73496993 None:intergenic 25.0%
!! CTTCATAGCACTATATATTT+TGG - Chr2:73496482-73496501 None:intergenic 25.0%
!! TATTTTCTAATGACTCTGTT+CGG - Chr2:73495920-73495939 None:intergenic 25.0%
!! TCTTAGTTACCTTAGAATTT+TGG - Chr2:73498341-73498360 None:intergenic 25.0%
!! TTTGGTGCGAAATTAATTAA+GGG - Chr2:73495221-73495240 None:intergenic 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + Chr2:73497959-73497978 MsG0280010696.01.T01:intron 25.0%
!!! AAATCCAGTTTTAGCTAAAA+TGG + Chr2:73496336-73496355 MsG0280010696.01.T01:CDS 25.0%
AAATGTTGAACAGAATGCAT+TGG + Chr2:73498116-73498135 MsG0280010696.01.T01:intron 30.0%
ACAATTCACACCAATTAATC+TGG - Chr2:73497610-73497629 None:intergenic 30.0%
AGAGTAGAAAAGGAAAACAA+AGG + Chr2:73497735-73497754 MsG0280010696.01.T01:intron 30.0%
AGGTGATTATTGTACATCTT+TGG + Chr2:73496604-73496623 MsG0280010696.01.T01:intron 30.0%
ATTGTTCATCCTTGAATTGT+TGG - Chr2:73497233-73497252 None:intergenic 30.0%
GAAATTAATTAAGGGATTGG+TGG - Chr2:73495213-73495232 None:intergenic 30.0%
GAAGAAGTTGAAAACTGATT+AGG + Chr2:73496225-73496244 MsG0280010696.01.T01:intron 30.0%
GATTAGGGTTTCATAATTCA+GGG + Chr2:73496241-73496260 MsG0280010696.01.T01:intron 30.0%
GGAGAAAATTGATGAAAGAA+AGG + Chr2:73497180-73497199 MsG0280010696.01.T01:intron 30.0%
GTATGAGATACTTATCCAAA+AGG - Chr2:73497666-73497685 None:intergenic 30.0%
TAGTTCAAACCAACAATTCA+AGG + Chr2:73497221-73497240 MsG0280010696.01.T01:intron 30.0%
TCTAAACAAACAATTGTCTG+TGG - Chr2:73497281-73497300 None:intergenic 30.0%
TGATTAGGGTTTCATAATTC+AGG + Chr2:73496240-73496259 MsG0280010696.01.T01:intron 30.0%
TGATTGAACTTAGCTTATAG+AGG + Chr2:73495644-73495663 MsG0280010696.01.T01:intron 30.0%
TGCGAAATTAATTAAGGGAT+TGG - Chr2:73495216-73495235 None:intergenic 30.0%
TGTATTATAGTAAAGCAGAC+GGG + Chr2:73496157-73496176 MsG0280010696.01.T01:intron 30.0%
TTAGCTAAAATGGCAATAAG+TGG + Chr2:73496346-73496365 MsG0280010696.01.T01:CDS 30.0%
TTTGACTATAATGATCTGTC+AGG - Chr2:73496081-73496100 None:intergenic 30.0%
TTTGATTCTACAGAGAAAGA+AGG - Chr2:73495182-73495201 None:intergenic 30.0%
! AATATTTCGCAAAGCATCAA+GGG - Chr2:73498078-73498097 None:intergenic 30.0%
! AATTGGATTTTGCGTTCATT+GGG + Chr2:73498168-73498187 MsG0280010696.01.T01:intron 30.0%
! ATTGAGTTGTTTATCCAAAG+AGG + Chr2:73495955-73495974 MsG0280010696.01.T01:intron 30.0%
! TAATTGGATTTTGCGTTCAT+TGG + Chr2:73498167-73498186 MsG0280010696.01.T01:intron 30.0%
!! CTCAGGTATGTTTTTGAATT+TGG + Chr2:73496398-73496417 MsG0280010696.01.T01:intron 30.0%
!! GTTTGGTGCGAAATTAATTA+AGG - Chr2:73495222-73495241 None:intergenic 30.0%
!! TATTTAGATCAGGTCTTGTT+GGG + Chr2:73496535-73496554 MsG0280010696.01.T01:intron 30.0%
!!! ATTGCCATTTTAGCTAAAAC+TGG - Chr2:73496343-73496362 None:intergenic 30.0%
!!! GATTTTTTATCTACTGGCAT+AGG + Chr2:73496905-73496924 MsG0280010696.01.T01:intron 30.0%
!!! TACATCTTTGGTTTGGATTT+TGG + Chr2:73496616-73496635 MsG0280010696.01.T01:intron 30.0%
!!! TTGGTTTTTTTCCCTAGTTT+GGG - Chr2:73497586-73497605 None:intergenic 30.0%
!!! TTTGGTTTTTTTCCCTAGTT+TGG - Chr2:73497587-73497606 None:intergenic 30.0%
!!! TTTTAAGTTCTTTGAGTCGT+TGG + Chr2:73497632-73497651 MsG0280010696.01.T01:intron 30.0%
AAAAGACAGAAAAGTCCTTG+AGG + Chr2:73495421-73495440 MsG0280010696.01.T01:CDS 35.0%
AATAGAAGAAGCAACTGACA+TGG - Chr2:73495276-73495295 None:intergenic 35.0%
AATAGTGAGAAAGAGATCCA+TGG - Chr2:73496569-73496588 None:intergenic 35.0%
AATATGTGCCTGAACACAAT+TGG + Chr2:73496287-73496306 MsG0280010696.01.T01:CDS 35.0%
ATAAGTTCTTACAGTGAGCA+TGG - Chr2:73496860-73496879 None:intergenic 35.0%
ATCTCATATTCACACCTCTT+TGG - Chr2:73495972-73495991 None:intergenic 35.0%
ATGATCAATGGTGAGCAATT+TGG - Chr2:73495558-73495577 None:intergenic 35.0%
ATGCCAGTAATCATGATCAA+TGG - Chr2:73495570-73495589 None:intergenic 35.0%
ATTACAGCATGTGAACAAAG+AGG - Chr2:73497083-73497102 None:intergenic 35.0%
ATTTCGTGATAAGTTTCACG+AGG - Chr2:73498410-73498429 None:intergenic 35.0%
CACACCATAAGTAATCAGAT+GGG - Chr2:73497379-73497398 None:intergenic 35.0%
GAAAAGGAAAACAAAGGAGA+AGG + Chr2:73497741-73497760 MsG0280010696.01.T01:intron 35.0%
GACTCTTTGGATAACATCTA+TGG + Chr2:73496811-73496830 MsG0280010696.01.T01:CDS 35.0%
GCGAAATATTGAGAGTTTGT+CGG + Chr2:73498088-73498107 MsG0280010696.01.T01:intron 35.0%
GCTCGATCAAACTCAAAAAT+AGG - Chr2:73496510-73496529 None:intergenic 35.0%
GTATTATAGTAAAGCAGACG+GGG + Chr2:73496158-73496177 MsG0280010696.01.T01:intron 35.0%
TAAGTTCTTACAGTGAGCAT+GGG - Chr2:73496859-73496878 None:intergenic 35.0%
TACACCAAGCCAAAATTCTA+AGG + Chr2:73498329-73498348 MsG0280010696.01.T01:CDS 35.0%
TCACACCAATTAATCTGGTT+TGG - Chr2:73497605-73497624 None:intergenic 35.0%
TCACCATTGATCATGATTAC+TGG + Chr2:73495564-73495583 MsG0280010696.01.T01:CDS 35.0%
TGCTTATAATAGTGACCAGA+GGG - Chr2:73497846-73497865 None:intergenic 35.0%
TTCACTCTAATATGCTTCAC+AGG + Chr2:73497329-73497348 MsG0280010696.01.T01:intron 35.0%
TTCATTATTGTAGCCGATTG+CGG - Chr2:73497789-73497808 None:intergenic 35.0%
TTGCTTATAATAGTGACCAG+AGG - Chr2:73497847-73497866 None:intergenic 35.0%
TTGTCTACTCAATTGGAGAT+TGG + Chr2:73496371-73496390 MsG0280010696.01.T01:CDS 35.0%
! ACCATCTACTTTGTGGTTTT+GGG + Chr2:73496775-73496794 MsG0280010696.01.T01:CDS 35.0%
! ATGAAGTGCTTATGTTGGAT+AGG + Chr2:73496433-73496452 MsG0280010696.01.T01:intron 35.0%
! CAATATTTCGCAAAGCATCA+AGG - Chr2:73498079-73498098 None:intergenic 35.0%
! CTATTTAGATCAGGTCTTGT+TGG + Chr2:73496534-73496553 MsG0280010696.01.T01:intron 35.0%
! CTCTTTTTCCTTTCCAACAA+TGG + Chr2:73496695-73496714 MsG0280010696.01.T01:CDS 35.0%
! GGTTCTAGTTGAGAATTGAT+TGG - Chr2:73495161-73495180 None:intergenic 35.0%
! TGAAGTGCTTATGTTGGATA+GGG + Chr2:73496434-73496453 MsG0280010696.01.T01:intron 35.0%
! TTTTCCTTTCCAACAATGGT+GGG + Chr2:73496699-73496718 MsG0280010696.01.T01:CDS 35.0%
! TTTTTCCTTTCCAACAATGG+TGG + Chr2:73496698-73496717 MsG0280010696.01.T01:CDS 35.0%
!!! GTTACCTTAGAATTTTGGCT+TGG - Chr2:73498336-73498355 None:intergenic 35.0%
!!! TATGATACGACGTCGTTTTT+TGG - Chr2:73495357-73495376 None:intergenic 35.0%
!!! TTTTTGGTTTGGGAAGAGAA+AGG - Chr2:73495341-73495360 None:intergenic 35.0%
AAACTGTGTGGTCAGCTATT+TGG + Chr2:73496749-73496768 MsG0280010696.01.T01:CDS 40.0%
AAATAGCTGACCACACAGTT+TGG - Chr2:73496750-73496769 None:intergenic 40.0%
AATTGGAGATTGGATTGCTC+AGG + Chr2:73496381-73496400 MsG0280010696.01.T01:CDS 40.0%
ACCCAAAACCACAAAGTAGA+TGG - Chr2:73496779-73496798 None:intergenic 40.0%
AGGGTTTCATAATTCAGGGT+TGG + Chr2:73496245-73496264 MsG0280010696.01.T01:intron 40.0%
AGTGGAGTTGTCTACTCAAT+TGG + Chr2:73496364-73496383 MsG0280010696.01.T01:CDS 40.0%
ATCAATGCTGCAATCGTTCT+CGG + Chr2:73495512-73495531 MsG0280010696.01.T01:CDS 40.0%
ATCAGAATCCCGAAAATCTG+AGG + Chr2:73498281-73498300 MsG0280010696.01.T01:CDS 40.0%
CAATAATCACCTCACACAGT+TGG - Chr2:73496596-73496615 None:intergenic 40.0%
CCACACCATAAGTAATCAGA+TGG - Chr2:73497380-73497399 None:intergenic 40.0%
CCATCTGATTACTTATGGTG+TGG + Chr2:73497377-73497396 MsG0280010696.01.T01:CDS 40.0%
CGTTGCCGTGTTTCTTAATT+TGG + Chr2:73496049-73496068 MsG0280010696.01.T01:intron 40.0%
CTATTACTACCAACTGTGTG+AGG + Chr2:73496584-73496603 MsG0280010696.01.T01:intron 40.0%
GAGTAAGTATATACTCCCTC+TGG + Chr2:73497828-73497847 MsG0280010696.01.T01:intron 40.0%
GAGTTCAATAAGACCGCAAT+CGG + Chr2:73497773-73497792 MsG0280010696.01.T01:intron 40.0%
GGTCAAAAGCAACTTTAGCA+GGG - Chr2:73496729-73496748 None:intergenic 40.0%
TAAGTAATCAGATGGGCGAA+AGG - Chr2:73497372-73497391 None:intergenic 40.0%
TAGGCAATCCAATTGTGTTC+AGG - Chr2:73496298-73496317 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG + Chr2:73497963-73497982 MsG0280010696.01.T01:intron 40.0%
TGCTTTCAGAGATTGCTCAT+AGG - Chr2:73496317-73496336 None:intergenic 40.0%
TGGTCAAAAGCAACTTTAGC+AGG - Chr2:73496730-73496749 None:intergenic 40.0%
TGTGGAACAAAGGCTTCTTT+GGG + Chr2:73497404-73497423 MsG0280010696.01.T01:CDS 40.0%
TTCGCCCATCTGATTACTTA+TGG + Chr2:73497372-73497391 MsG0280010696.01.T01:CDS 40.0%
TTGCTTATAATAGTGACCGG+AGG + Chr2:73497962-73497981 MsG0280010696.01.T01:intron 40.0%
TTGGAACACCATCTACTTTG+TGG + Chr2:73496768-73496787 MsG0280010696.01.T01:CDS 40.0%
TTGTTTGCGTTGTGTAACAC+AGG + Chr2:73496672-73496691 MsG0280010696.01.T01:intron 40.0%
TTTGTTGCGTTTCGACTCTT+TGG + Chr2:73496798-73496817 MsG0280010696.01.T01:CDS 40.0%
! CACCATCTACTTTGTGGTTT+TGG + Chr2:73496774-73496793 MsG0280010696.01.T01:CDS 40.0%
! CTGACACTTCCTCAGATTTT+CGG - Chr2:73498293-73498312 None:intergenic 40.0%
! TGACACTTCCTCAGATTTTC+GGG - Chr2:73498292-73498311 None:intergenic 40.0%
!! TTGCTTTTGACCAAACTGTG+TGG + Chr2:73496737-73496756 MsG0280010696.01.T01:CDS 40.0%
!!! ACGACGTCGTTTTTTGGTTT+GGG - Chr2:73495351-73495370 None:intergenic 40.0%
!!! TACGACGTCGTTTTTTGGTT+TGG - Chr2:73495352-73495371 None:intergenic 40.0%
!!! TTTTTGAGTTTGATCGAGCG+CGG + Chr2:73496511-73496530 MsG0280010696.01.T01:intron 40.0%
AAACATTGAGCGGAATGCGT+TGG + Chr2:73497559-73497578 MsG0280010696.01.T01:intron 45.0%
AAGGCGAAGAAGAATCCTCA+AGG - Chr2:73495439-73495458 None:intergenic 45.0%
AATGCGTTGGACCCAAACTA+GGG + Chr2:73497572-73497591 MsG0280010696.01.T01:intron 45.0%
ACAACCCACCATTGTTGGAA+AGG - Chr2:73496706-73496725 None:intergenic 45.0%
ACCTCTGTAATACCCTTGGA+AGG - Chr2:73495458-73495477 None:intergenic 45.0%
AGCAACCTCTGTAATACCCT+TGG - Chr2:73495462-73495481 None:intergenic 45.0%
ATGAAAGAAAGGGGTCAGAG+AGG + Chr2:73497191-73497210 MsG0280010696.01.T01:intron 45.0%
CAAAGAAGCCTTTGTTCCAC+AGG - Chr2:73497405-73497424 None:intergenic 45.0%
CATAATCAGCTCCGTTACAG+AGG + Chr2:73495373-73495392 MsG0280010696.01.T01:CDS 45.0%
CTCTAATATGCTTCACAGGC+AGG + Chr2:73497333-73497352 MsG0280010696.01.T01:intron 45.0%
CTGTGGAACAAAGGCTTCTT+TGG + Chr2:73497403-73497422 MsG0280010696.01.T01:CDS 45.0%
CTTGTTGGGTTTACTCTCCA+TGG + Chr2:73496549-73496568 MsG0280010696.01.T01:intron 45.0%
GATTCTTCTTCGCCTTCCAA+GGG + Chr2:73495443-73495462 MsG0280010696.01.T01:CDS 45.0%
GGACTGTATTGAGCTCATCT+GGG + Chr2:73497428-73497447 MsG0280010696.01.T01:CDS 45.0%
TAAGTTTCACGAGGACTCTC+TGG - Chr2:73498401-73498420 None:intergenic 45.0%
TACTTATGGTGTGGTTCCTG+TGG + Chr2:73497386-73497405 MsG0280010696.01.T01:CDS 45.0%
TAGGCACTTGCGAAACTCTT+TGG + Chr2:73496924-73496943 MsG0280010696.01.T01:intron 45.0%
TCCGCTCAATGTTTCAGTGT+GGG - Chr2:73497553-73497572 None:intergenic 45.0%
TGCTTATTGTGTACTCCCTC+CGG - Chr2:73497981-73498000 None:intergenic 45.0%
TGCTTCACGATCCTCTGTAA+CGG - Chr2:73495387-73495406 None:intergenic 45.0%
TGGACTGTATTGAGCTCATC+TGG + Chr2:73497427-73497446 MsG0280010696.01.T01:CDS 45.0%
TTATAGTAAAGCAGACGGGG+AGG + Chr2:73496161-73496180 MsG0280010696.01.T01:intron 45.0%
TTCCGCTCAATGTTTCAGTG+TGG - Chr2:73497554-73497573 None:intergenic 45.0%
TTGGGAAGAGAAAGGAGGTA+GGG - Chr2:73495333-73495352 None:intergenic 45.0%
TTTGGGAAGAGAAAGGAGGT+AGG - Chr2:73495334-73495353 None:intergenic 45.0%
! AAAGCATCAAGGGTTTGAGG+GGG - Chr2:73498068-73498087 None:intergenic 45.0%
! CAAAGCATCAAGGGTTTGAG+GGG - Chr2:73498069-73498088 None:intergenic 45.0%
! GAGAACGATTGCAGCATTGA+TGG - Chr2:73495513-73495532 None:intergenic 45.0%
! GCAAAGCATCAAGGGTTTGA+GGG - Chr2:73498070-73498089 None:intergenic 45.0%
!! GAGGAAGTGTCAGAAACAAC+AGG + Chr2:73498300-73498319 MsG0280010696.01.T01:CDS 45.0%
!! TTGGTTTGGGAAGAGAAAGG+AGG - Chr2:73495338-73495357 None:intergenic 45.0%
!!! TCGTTGGTTCAATGCCCTTT+TGG + Chr2:73497648-73497667 MsG0280010696.01.T01:intron 45.0%
AAAGGAGGTAGGGAGATGTG+GGG - Chr2:73495323-73495342 None:intergenic 50.0%
AATATGCTTCACAGGCAGGC+TGG + Chr2:73497337-73497356 MsG0280010696.01.T01:intron 50.0%
AGAAAGGAGGTAGGGAGATG+TGG - Chr2:73495325-73495344 None:intergenic 50.0%
AGAGGATCGTGAAGCAGCTT+CGG + Chr2:73495391-73495410 MsG0280010696.01.T01:CDS 50.0%
GAAAGGAGGTAGGGAGATGT+GGG - Chr2:73495324-73495343 None:intergenic 50.0%
GAATGCGTTGGACCCAAACT+AGG + Chr2:73497571-73497590 MsG0280010696.01.T01:intron 50.0%
GCCCACACTGAAACATTGAG+CGG + Chr2:73497549-73497568 MsG0280010696.01.T01:intron 50.0%
GCTGACACTGATATCCTCAC+CGG + Chr2:73495482-73495501 MsG0280010696.01.T01:CDS 50.0%
GCTGCAATCGTTCTCGGATT+TGG + Chr2:73495518-73495537 MsG0280010696.01.T01:CDS 50.0%
GGAACAAAGGCTTCTTTGGG+TGG + Chr2:73497407-73497426 MsG0280010696.01.T01:CDS 50.0%
GGATTCTTCTTCGCCTTCCA+AGG + Chr2:73495442-73495461 MsG0280010696.01.T01:CDS 50.0%
GTGTGGTTCCTGTGGAACAA+AGG + Chr2:73497394-73497413 MsG0280010696.01.T01:CDS 50.0%
! CGCAAAGCATCAAGGGTTTG+AGG - Chr2:73498071-73498090 None:intergenic 50.0%
!! GAGATGTGGGGCTTTGAGAA+GGG - Chr2:73495311-73495330 None:intergenic 50.0%
!! GCCTTCCAAGGGTATTACAG+AGG + Chr2:73495454-73495473 MsG0280010696.01.T01:CDS 50.0%
AGTAAAGCAGACGGGGAGGA+TGG + Chr2:73496165-73496184 MsG0280010696.01.T01:intron 55.0%
CAGGGACAACCCACCATTGT+TGG - Chr2:73496711-73496730 None:intergenic 55.0%
CGAGCGCGGCTATTTAGATC+AGG + Chr2:73496525-73496544 MsG0280010696.01.T01:intron 55.0%
!! GGAGATGTGGGGCTTTGAGA+AGG - Chr2:73495312-73495331 None:intergenic 55.0%
!! GGGCTTTGAGAAGGGAAGGA+GGG - Chr2:73495303-73495322 None:intergenic 55.0%
!! TGCAGCATTGATGGCACGTC+CGG - Chr2:73495504-73495523 None:intergenic 55.0%
!! TGTGGGGCTTTGAGAAGGGA+AGG - Chr2:73495307-73495326 None:intergenic 55.0%
CACAGGCAGGCTGGAAGCTT+TGG + Chr2:73497346-73497365 MsG0280010696.01.T01:intron 60.0%
! CATTGATGGCACGTCCGGTG+AGG - Chr2:73495499-73495518 None:intergenic 60.0%
!! GGGGCTTTGAGAAGGGAAGG+AGG - Chr2:73495304-73495323 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 73495136 73498431 73495136 ID=MsG0280010696.01;Name=MsG0280010696.01
Chr2 mRNA 73495136 73498431 73495136 ID=MsG0280010696.01.T01;Parent=MsG0280010696.01;Name=MsG0280010696.01.T01;_AED=0.41;_eAED=0.46;_QI=133|0.75|0.8|1|1|1|5|0|305
Chr2 exon 73495136 73495589 73495136 ID=MsG0280010696.01.T01:exon:7279;Parent=MsG0280010696.01.T01
Chr2 exon 73496262 73496402 73496262 ID=MsG0280010696.01.T01:exon:7280;Parent=MsG0280010696.01.T01
Chr2 exon 73496694 73496867 73496694 ID=MsG0280010696.01.T01:exon:7281;Parent=MsG0280010696.01.T01
Chr2 exon 73497351 73497466 73497351 ID=MsG0280010696.01.T01:exon:7282;Parent=MsG0280010696.01.T01
Chr2 exon 73498266 73498431 73498266 ID=MsG0280010696.01.T01:exon:7283;Parent=MsG0280010696.01.T01
Chr2 five_prime_UTR 73495136 73495268 73495136 ID=MsG0280010696.01.T01:five_prime_utr;Parent=MsG0280010696.01.T01
Chr2 CDS 73495269 73495589 73495269 ID=MsG0280010696.01.T01:cds;Parent=MsG0280010696.01.T01
Chr2 CDS 73496262 73496402 73496262 ID=MsG0280010696.01.T01:cds;Parent=MsG0280010696.01.T01
Chr2 CDS 73496694 73496867 73496694 ID=MsG0280010696.01.T01:cds;Parent=MsG0280010696.01.T01
Chr2 CDS 73497351 73497466 73497351 ID=MsG0280010696.01.T01:cds;Parent=MsG0280010696.01.T01
Chr2 CDS 73498266 73498431 73498266 ID=MsG0280010696.01.T01:cds;Parent=MsG0280010696.01.T01
Gene Sequence

>MsG0280010696.01.T01

ATGGCCATGTCAGTTGCTTCTTCTATTTGCACCCTCCTTCCCTTCTCAAAGCCCCACATCTCCCTACCTCCTTTCTCTTCCCAAACCAAAAAACGACGTCGTATCATAATCAGCTCCGTTACAGAGGATCGTGAAGCAGCTTCGGTTGTGTCAAAAGACAGAAAAGTCCTTGAGGATTCTTCTTCGCCTTCCAAGGGTATTACAGAGGTTGCTGCTGACACTGATATCCTCACCGGACGTGCCATCAATGCTGCAATCGTTCTCGGATTTGGAGCTTTTGCTGTCACCAAATTGCTCACCATTGATCATGATTACTGGCATGGTTGGACACTTTATGAGATACTGAAATATGTGCCTGAACACAATTGGATTGCCTATGAGCAATCTCTGAAAGCAAATCCAGTTTTAGCTAAAATGGCAATAAGTGGAGTTGTCTACTCAATTGGAGATTGGATTGCTCAGGCTCTTTTTCCTTTCCAACAATGGTGGGTTGTCCCTGCTAAAGTTGCTTTTGACCAAACTGTGTGGTCAGCTATTTGGAACACCATCTACTTTGTGGTTTTGGGTTTGTTGCGTTTCGACTCTTTGGATAACATCTATGGTGAACTCAAATCAACATTTTTTCCCATGCTCACTGCAGGCTGGAAGCTTTGGCCTTTCGCCCATCTGATTACTTATGGTGTGGTTCCTGTGGAACAAAGGCTTCTTTGGGTGGACTGTATTGAGCTCATCTGGGTCACAATACTTTCAACGTATTCAAATGAGAAATCAGAATCCCGAAAATCTGAGGAAGTGTCAGAAACAACAGGATCATCTACACCAAGCCAAAATTCTAAGGTAACTAAGAAGACCGTTGTATTATTTTTCTGTCATGTTTTAAGGTTCCAGAGAGTCCTCGTGAAACTTATCACGAAATGA
Protein sequence

>MsG0280010696.01.T01

MAMSVASSICTLLPFSKPHISLPPFSSQTKKRRRIIISSVTEDREAASVVSKDRKVLEDSSSPSKGITEVAADTDILTGRAINAAIVLGFGAFAVTKLLTIDHDYWHGWTLYEILKYVPEHNWIAYEQSLKANPVLAKMAISGVVYSIGDWIAQALFPFQQWWVVPAKVAFDQTVWSAIWNTIYFVVLGLLRFDSLDNIYGELKSTFFPMLTAGWKLWPFAHLITYGVVPVEQRLLWVDCIELIWVTILSTYSNEKSESRKSEEVSETTGSSTPSQNSKVTKKTVVLFFCHVLRFQRVLVKLITK*