AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016066.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016066.01.T01 MTR_3g082750 92.391 184 14 0 1 184 1 184 6.34e-119 353
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016066.01.T01 AT3G07700 50.000 190 72 8 1 184 1 173 1.83e-46 162
MsG0380016066.01.T01 AT3G07700 50.000 190 72 8 1 184 1 173 1.83e-46 162
MsG0380016066.01.T01 AT3G07700 50.000 190 72 8 1 184 1 173 1.83e-46 162
MsG0380016066.01.T01 AT3G07700 50.000 190 72 8 1 184 1 173 2.69e-46 162

Find 35 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTCAAAGCAAGCTGTTAA+TGG 0.283062 3:-78624165 MsG0380016066.01.T01:CDS
GAAATGCCGCCATCAAAATT+AGG 0.325630 3:-78624276 MsG0380016066.01.T01:CDS
ACCCTAAATGGCAGTAATAA+TGG 0.329958 3:-78624475 None:intergenic
AGCTGTTAATGGAGCAAGTT+TGG 0.341642 3:-78624154 MsG0380016066.01.T01:CDS
TTCGTTGTCTTTGTCAATGC+TGG 0.352992 3:+78624114 None:intergenic
ACAATGCAAAATGGGCATAT+CGG 0.381897 3:-78623936 MsG0380016066.01.T01:CDS
CCAATGAAAATTACAGTTCC+TGG 0.383016 3:-78624017 MsG0380016066.01.T01:CDS
TTCAAGTGGTGGAAGTTCAT+TGG 0.384033 3:+78624080 None:intergenic
TAATGGTAGAGCTGTTAAAA+TGG 0.385147 3:-78624250 MsG0380016066.01.T01:CDS
CCATCAAAATTAGGTAATAA+TGG 0.389209 3:-78624267 MsG0380016066.01.T01:CDS
CGAAGATCACTAGACAATCT+TGG 0.421184 3:-78624408 MsG0380016066.01.T01:CDS
CAATGCAAAATGGGCATATC+GGG 0.428931 3:-78623935 MsG0380016066.01.T01:CDS
GGAAGTTCATTGGAGATTGT+AGG 0.432975 3:+78624090 None:intergenic
CCTCAGATGAAGGCTTCAGT+TGG 0.474873 3:-78624041 MsG0380016066.01.T01:CDS
TGGTAAATTTCGTAGATTTG+TGG 0.513813 3:-78624316 MsG0380016066.01.T01:CDS
GGCAGAGATCAATTGATGTA+TGG 0.520886 3:-78623996 MsG0380016066.01.T01:CDS
CTCAGATGAAGGCTTCAGTT+GGG 0.526088 3:-78624040 MsG0380016066.01.T01:CDS
TTATTGTAACAATATAGAAT+CGG 0.529878 3:-78624439 MsG0380016066.01.T01:CDS
TAAATTTCGTAGATTTGTGG+TGG 0.532222 3:-78624313 MsG0380016066.01.T01:CDS
GTCTCCACTTTATTCTCCGA+CGG 0.532763 3:+78624195 None:intergenic
GTCTTTGTCAATGCTGGAGT+AGG 0.534650 3:+78624120 None:intergenic
CCAACTGAAGCCTTCATCTG+AGG 0.557528 3:+78624041 None:intergenic
ATAAATTGTCAAAGAATGAA+AGG 0.558325 3:-78624362 MsG0380016066.01.T01:CDS
CAACTGAAGCCTTCATCTGA+GGG 0.559152 3:+78624042 None:intergenic
AATAAAGTGGAGACAGTCAA+TGG 0.564916 3:-78624186 MsG0380016066.01.T01:CDS
TAGAGCTGTTAAAATGGTAC+CGG 0.578175 3:-78624244 MsG0380016066.01.T01:CDS
TACATCAATTGATCTCTGCC+AGG 0.581512 3:+78623999 None:intergenic
TGGCTTCACAGAAGAAAAGC+AGG 0.586577 3:-78623912 MsG0380016066.01.T01:CDS
GACACCGTCGGAGAATAAAG+TGG 0.590443 3:-78624199 MsG0380016066.01.T01:CDS
TGCAAAATGGGCATATCGGG+TGG 0.607669 3:-78623932 MsG0380016066.01.T01:CDS
ACATCTGATAAGTTAAAGAG+TGG 0.637198 3:-78624336 MsG0380016066.01.T01:CDS
TTGCATTGTCGAAAAGAACA+CGG 0.647652 3:+78623950 None:intergenic
GCAGTAATAATGGCTTTGCA+TGG 0.669620 3:-78624465 MsG0380016066.01.T01:CDS
GACACGTAAAGAAAGAACAA+AGG 0.679744 3:+78623972 None:intergenic
TTCACAGAAGAAAAGCAGGT+AGG 0.687391 3:-78623908 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTGTAACAATATAGAAT+CGG - Chr3:78623938-78623957 MsG0380016066.01.T01:CDS 15.0%
!! ATAAATTGTCAAAGAATGAA+AGG - Chr3:78624015-78624034 MsG0380016066.01.T01:CDS 20.0%
! CCATCAAAATTAGGTAATAA+TGG - Chr3:78624110-78624129 MsG0380016066.01.T01:CDS 25.0%
!! CCATTATTACCTAATTTTGA+TGG + Chr3:78624113-78624132 None:intergenic 25.0%
!!! TTATTACCTAATTTTGATGG+CGG + Chr3:78624110-78624129 None:intergenic 25.0%
ACATCTGATAAGTTAAAGAG+TGG - Chr3:78624041-78624060 MsG0380016066.01.T01:CDS 30.0%
TAAATTTCGTAGATTTGTGG+TGG - Chr3:78624064-78624083 MsG0380016066.01.T01:CDS 30.0%
TAATGGTAGAGCTGTTAAAA+TGG - Chr3:78624127-78624146 MsG0380016066.01.T01:CDS 30.0%
TGGTAAATTTCGTAGATTTG+TGG - Chr3:78624061-78624080 MsG0380016066.01.T01:CDS 30.0%
! TCTTTTCGACAATGCAAAAT+GGG - Chr3:78624433-78624452 MsG0380016066.01.T01:CDS 30.0%
! TTCTTTTCGACAATGCAAAA+TGG - Chr3:78624432-78624451 MsG0380016066.01.T01:CDS 30.0%
!!! AAAACTTTTAGCTCTTCAAG+TGG + Chr3:78624314-78624333 None:intergenic 30.0%
AATAAAGTGGAGACAGTCAA+TGG - Chr3:78624191-78624210 MsG0380016066.01.T01:CDS 35.0%
ACAATGCAAAATGGGCATAT+CGG - Chr3:78624441-78624460 MsG0380016066.01.T01:CDS 35.0%
CCAATGAAAATTACAGTTCC+TGG - Chr3:78624360-78624379 MsG0380016066.01.T01:CDS 35.0%
GACACGTAAAGAAAGAACAA+AGG + Chr3:78624408-78624427 None:intergenic 35.0%
TAGAGCTGTTAAAATGGTAC+CGG - Chr3:78624133-78624152 MsG0380016066.01.T01:CDS 35.0%
TTGCATTGTCGAAAAGAACA+CGG + Chr3:78624430-78624449 None:intergenic 35.0%
! CCAGGAACTGTAATTTTCAT+TGG + Chr3:78624363-78624382 None:intergenic 35.0%
AGCAAAAAGAAAGACACCGT+CGG - Chr3:78624166-78624185 MsG0380016066.01.T01:CDS 40.0%
AGCTGTTAATGGAGCAAGTT+TGG - Chr3:78624223-78624242 MsG0380016066.01.T01:CDS 40.0%
CAATGCAAAATGGGCATATC+GGG - Chr3:78624442-78624461 MsG0380016066.01.T01:CDS 40.0%
CGAAGATCACTAGACAATCT+TGG - Chr3:78623969-78623988 MsG0380016066.01.T01:CDS 40.0%
GAAATGCCGCCATCAAAATT+AGG - Chr3:78624101-78624120 MsG0380016066.01.T01:CDS 40.0%
GGAAGTTCATTGGAGATTGT+AGG + Chr3:78624290-78624309 None:intergenic 40.0%
GGCAGAGATCAATTGATGTA+TGG - Chr3:78624381-78624400 MsG0380016066.01.T01:CDS 40.0%
TACATCAATTGATCTCTGCC+AGG + Chr3:78624381-78624400 None:intergenic 40.0%
TTCGTTGTCTTTGTCAATGC+TGG + Chr3:78624266-78624285 None:intergenic 40.0%
! AAAGTTTTGCCCTCAGATGA+AGG - Chr3:78624326-78624345 MsG0380016066.01.T01:CDS 40.0%
! GCAGTAATAATGGCTTTGCA+TGG - Chr3:78623912-78623931 MsG0380016066.01.T01:CDS 40.0%
! TTCAAGTGGTGGAAGTTCAT+TGG + Chr3:78624300-78624319 None:intergenic 40.0%
!! CTTTTTGCTATCTCAGTTGC+CGG + Chr3:78624155-78624174 None:intergenic 40.0%
!!! ACTTTTAGCTCTTCAAGTGG+TGG + Chr3:78624311-78624330 None:intergenic 40.0%
CAACTGAAGCCTTCATCTGA+GGG + Chr3:78624338-78624357 None:intergenic 45.0%
CTCAGATGAAGGCTTCAGTT+GGG - Chr3:78624337-78624356 MsG0380016066.01.T01:CDS 45.0%
GGCTCAAAGCAAGCTGTTAA+TGG - Chr3:78624212-78624231 MsG0380016066.01.T01:CDS 45.0%
GTCTCCACTTTATTCTCCGA+CGG + Chr3:78624185-78624204 None:intergenic 45.0%
GTCTTTGTCAATGCTGGAGT+AGG + Chr3:78624260-78624279 None:intergenic 45.0%
TGGCTTCACAGAAGAAAAGC+AGG - Chr3:78624465-78624484 MsG0380016066.01.T01:CDS 45.0%
CCAACTGAAGCCTTCATCTG+AGG + Chr3:78624339-78624358 None:intergenic 50.0%
CCTCAGATGAAGGCTTCAGT+TGG - Chr3:78624336-78624355 MsG0380016066.01.T01:CDS 50.0%
GACACCGTCGGAGAATAAAG+TGG - Chr3:78624178-78624197 MsG0380016066.01.T01:CDS 50.0%
TGCAAAATGGGCATATCGGG+TGG - Chr3:78624445-78624464 MsG0380016066.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 78623909 78624490 78623909 ID=MsG0380016066.01;Name=MsG0380016066.01
Chr3 mRNA 78623909 78624490 78623909 ID=MsG0380016066.01.T01;Parent=MsG0380016066.01;Name=MsG0380016066.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|193
Chr3 exon 78623909 78624490 78623909 ID=MsG0380016066.01.T01:exon:17525;Parent=MsG0380016066.01.T01
Chr3 CDS 78623909 78624490 78623909 ID=MsG0380016066.01.T01:cds;Parent=MsG0380016066.01.T01
Gene Sequence

>MsG0380016066.01.T01

ATGGCAGTAATAATGGCTTTGCATGGTTGTTATTGTAACAATATAGAATCGGTAAATCAGCGAAGATCACTAGACAATCTTGGTTTTACAAGCTCAATTTCATCTCATAAATTGTCAAAGAATGAAAGGTCAACATCTGATAAGTTAAAGAGTGGTAAATTTCGTAGATTTGTGGTGGAAATGCGACAGACAGAAATGCCGCCATCAAAATTAGGTAATAATGGTAGAGCTGTTAAAATGGTACCGGCAACTGAGATAGCAAAAAGAAAGACACCGTCGGAGAATAAAGTGGAGACAGTCAATGGCTCAAAGCAAGCTGTTAATGGAGCAAGTTTGGTAAGAAGAGATCCTACTCCAGCATTGACAAAGACAACGAAACCTACAATCTCCAATGAACTTCCACCACTTGAAGAGCTAAAAGTTTTGCCCTCAGATGAAGGCTTCAGTTGGGCCAATGAAAATTACAGTTCCTGGCAGAGATCAATTGATGTATGGTCCTTTGTTCTTTCTTTACGTGTCCGTGTTCTTTTCGACAATGCAAAATGGGCATATCGGGTGGCTTCACAGAAGAAAAGCAGGTAG

Protein sequence

>MsG0380016066.01.T01

MAVIMALHGCYCNNIESVNQRRSLDNLGFTSSISSHKLSKNERSTSDKLKSGKFRRFVVEMRQTEMPPSKLGNNGRAVKMVPATEIAKRKTPSENKVETVNGSKQAVNGASLVRRDPTPALTKTTKPTISNELPPLEELKVLPSDEGFSWANENYSSWQRSIDVWSFVLSLRVRVLFDNAKWAYRVASQKKSR*