AlfalfaGEDB | MsG0280011289.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011289.01

KEGG: K14553
GO: GO:0005515
PFAM: PF00400

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011289.01.T01	RHN71712.1	73.987	469	64	4	1	423	58	514	0	686

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011289.01.T01	Q9FMU5	52.929	495	153	7	1	423	60	546	5.83E-165	477

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011289.01.T01	Q2HU46	78.916	498	30	3	1	423	58	555	0.0	779

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280011289.01	MsG0480018401.01	0.810404	1.172513e-50	7.060952e-48
MsG0280011289.01	MsG0480023962.01	0.822706	2.052156e-53	1.730822e-50

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PPI

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Gene1	Gene2	Type
MsG0280011289.01	MsG0680035883.01	PPI
MsG0280011289.01	MsG0480022712.01	PPI
MsG0280011289.01	MsG0880046321.01	PPI
MsG0280011289.01	MsG0780039623.01	PPI
MsG0280011289.01	MsG0780041305.01	PPI
MsG0280011289.01	MsG0680030624.01	PPI
MsG0280011289.01	MsG0180004915.01	PPI
MsG0280011289.01	MsG0380014607.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011289.01.T01	MTR_2g101620	78.916	498	30	3	1	423	58	555	0.0	779
MsG0280011289.01.T01	MTR_2g009800	73.494	498	57	3	1	423	58	555	0.0	727

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011289.01.T01	AT5G14050	52.929	495	153	7	1	423	60	546	5.95e-166	477

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Find 102 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCATCTTTCTTGCCCGA+TGG	0.557386	2:+82030687	MsG0280011289.01.T01:CDS
AAAGCCGGTTTGGGTGGATG+AGG	0.539602	2:+82030166	MsG0280011289.01.T01:CDS
AAATATTAGCAATCTGTTCA+AGG	0.450551	2:+82031240	MsG0280011289.01.T01:CDS
AAATGGGGATAACGATGAAG+GGG	0.648646	2:+82030016	MsG0280011289.01.T01:CDS
AACATTCAGGATCCTTCTAA+TGG	0.448781	2:+82030523	MsG0280011289.01.T01:CDS
AAGAGGACGAGAATGTGATC+TGG	0.530284	2:+82030249	MsG0280011289.01.T01:CDS
AAGCATCTTTCTTGCCCGAT+GGG	0.479466	2:+82030688	MsG0280011289.01.T01:CDS
AAGGAAGTTAAGGAAGGAAG+AGG	0.502686	2:+82030232	MsG0280011289.01.T01:CDS
AAGTAATGTTGTTGCGAGCC+GGG	0.448192	2:+82030395	MsG0280011289.01.T01:CDS
AATAGTGAGGAATTTCTTGG+GGG	0.659821	2:+82031143	MsG0280011289.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAATAGTGAGGAATTTCTT+GGG	+	Chr2:82031141-82031160	MsG0280011289.01.T01:CDS	30.0%
CTGGACTAAAATCAATACAA+CGG	-	Chr2:82031355-82031374	None:intergenic	30.0%
CTTAGATTCTTTCAAATCGA+TGG	+	Chr2:82030613-82030632	MsG0280011289.01.T01:CDS	30.0%
CTTCCTTAATCTGTTAACTT+TGG	-	Chr2:82030219-82030238	None:intergenic	30.0%
TACAATAGTGAGGAATTTCT+TGG	+	Chr2:82031140-82031159	MsG0280011289.01.T01:CDS	30.0%
TAGCCAAAGTTAACAGATTA+AGG	+	Chr2:82030213-82030232	MsG0280011289.01.T01:CDS	30.0%
TGGACTAAAATCAATACAAC+GGG	-	Chr2:82031354-82031373	None:intergenic	30.0%
TTCTAATGGTCCCATTAATT+CGG	+	Chr2:82030537-82030556	MsG0280011289.01.T01:CDS	30.0%
AACATTCAGGATCCTTCTAA+TGG	+	Chr2:82030523-82030542	MsG0280011289.01.T01:CDS	35.0%
AATAGTGAGGAATTTCTTGG+GGG	+	Chr2:82031143-82031162	MsG0280011289.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	82029960	82031451	82029960	ID=MsG0280011289.01;Name=MsG0280011289.01
Chr2	mRNA	82029960	82031451	82029960	ID=MsG0280011289.01.T01;Parent=MsG0280011289.01;Name=MsG0280011289.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|0\|0\|1\|1\|1\|4\|0\|423
Chr2	exon	82029960	82030277	82029960	ID=MsG0280011289.01.T01:exon:12198;Parent=MsG0280011289.01.T01
Chr2	exon	82030339	82030417	82030339	ID=MsG0280011289.01.T01:exon:12199;Parent=MsG0280011289.01.T01
Chr2	exon	82030491	82030716	82030491	ID=MsG0280011289.01.T01:exon:12200;Parent=MsG0280011289.01.T01
Chr2	exon	82030803	82031451	82030803	ID=MsG0280011289.01.T01:exon:12201;Parent=MsG0280011289.01.T01
Chr2	CDS	82029960	82030277	82029960	ID=MsG0280011289.01.T01:cds;Parent=MsG0280011289.01.T01
Chr2	CDS	82030339	82030417	82030339	ID=MsG0280011289.01.T01:cds;Parent=MsG0280011289.01.T01
Chr2	CDS	82030491	82030716	82030491	ID=MsG0280011289.01.T01:cds;Parent=MsG0280011289.01.T01
Chr2	CDS	82030803	82031451	82030803	ID=MsG0280011289.01.T01:cds;Parent=MsG0280011289.01.T01

Gene Sequence

>MsG0280011289.01.T01

ATGAAAAAACTTGAAAGCTTTTTGTTTGGGTCTCTCTACTCCCCTCTTGAATTTGGAAATGGGGATAACGATGAAGGGGAAGCCATGACTGCTGCAAAGGCTTCCAATTTGTTCTTCACTGACCGTTCTGCTGATAGTGGCCTCACTGTTTACCAAGAAGATTCTGACTTCTCGGATGGAAGTTGTGATGATGATGCTTTGCGAAGAAAGCCGGTTTGGGTGGATGAGGAAGAAGAGAAAGTCACTGTTAACATAGCCAAAGTTAACAGATTAAGGAAGTTAAGGAAGGAAGAGGACGAGAATGTGATCTGGTTCAGACTTGATTCAGGGTCAAAAATGGATGGATCTTCTGATGATGAATTGACAGATGATGGAAGTAATGTTGTTGCGAGCCGGGGTCATATTGAATACTCGAAACTTGTAGATGCAAACATTCAGGATCCTTCTAATGGTCCCATTAATTCGGTTCAGTTCCATCGAAATGGTCAGCTGCTTCTTGCTGCTGGATTAGACCGGAATCTTAGATTCTTTCAAATCGATGGCAAACGGAACACCAAATTCAAAGCATCTTCCTTGAAGATTGCCCCTTTCGAAAAGCATCTTTCTTGCCCGATGGGTCTCAAGGAAGAGAAGAGTATTGAAGTTTTTGAGGTCTCTCCTGATTCTAAAATGATAGCCTTTGTGGGTGATGAAGGTTATATTTTACTGGTTTCAACAAAATCAAAGCAATTAGTTGGTACTCTGAAGATGAACGGAACAGCCCGTGCGTTAGCTTTTACTGAAGATGGACAAAAGTTGTTGAGCACCGGTGGTGATGGCCATGTTTACCATTGGGATCTGAGAACAAGGACTTGTATGCACAAAGGTGTAGATGAAGGCTGTTTAAACGGCACAGCCCTTTGTACTTCTCCAGTAGGGACACATTTTGCAGCTGGTTCGGCTAGTGGAGTTGTGAATATTTACAATAGTGAGGAATTTCTTGGGGGAAAGAGAAAACCTATCAAGACCATTGATAACCTGAATACTGAAGTGGATTTCATGAAATTTAATCATGACTCTCAAATATTAGCAATCTGTTCAAGGTCAAAGAATAGTAGTTTGAAATTAATACATATACCATCCTACACTGTTTTCTCTAACTGGCCGCGTTCAAATGCGAGCCTGCATTATCCCCGTTGTATTGATTTTAGTCCAGGTGGTGGTTTTATGGCTGTAGGAAACGCTTCAGGCAAAGTGTTACTATATAAGTTGCATCATTATCACCATGCCTAA

Protein sequence

>MsG0280011289.01.T01

MKKLESFLFGSLYSPLEFGNGDNDEGEAMTAAKASNLFFTDRSADSGLTVYQEDSDFSDGSCDDDALRRKPVWVDEEEEKVTVNIAKVNRLRKLRKEEDENVIWFRLDSGSKMDGSSDDELTDDGSNVVASRGHIEYSKLVDANIQDPSNGPINSVQFHRNGQLLLAAGLDRNLRFFQIDGKRNTKFKASSLKIAPFEKHLSCPMGLKEEKSIEVFEVSPDSKMIAFVGDEGYILLVSTKSKQLVGTLKMNGTARALAFTEDGQKLLSTGGDGHVYHWDLRTRTCMHKGVDEGCLNGTALCTSPVGTHFAAGSASGVVNIYNSEEFLGGKRKPIKTIDNLNTEVDFMKFNHDSQILAICSRSKNSSLKLIHIPSYTVFSNWPRSNASLHYPRCIDFSPGGGFMAVGNASGKVLLYKLHHYHHA*