AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011289.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011289.01.T01 MTR_2g101620 78.916 498 30 3 1 423 58 555 0.0 779
MsG0280011289.01.T01 MTR_2g009800 73.494 498 57 3 1 423 58 555 0.0 727
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011289.01.T01 AT5G14050 52.929 495 153 7 1 423 60 546 5.95e-166 477

Find 102 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTACTCCCCTCTTGAATT+TGG 0.174548 2:+82029993 MsG0280011289.01.T01:CDS
CAAGGAAGATGCTTTGAATT+TGG 0.186731 2:-82030647 None:intergenic
ATGGAACTGAACCGAATTAA+TGG 0.250496 2:-82030548 None:intergenic
TGAAATCCACTTCAGTATTC+AGG 0.253259 2:-82031196 None:intergenic
TAGCCAAAGTTAACAGATTA+AGG 0.262384 2:+82030213 MsG0280011289.01.T01:CDS
GACTGGGCACAGCTTGATTC+AGG 0.267674 2:+82030327 MsG0280011289.01.T01:intron
CCGAGAAGTCAGAATCTTCT+TGG 0.315985 2:-82030112 None:intergenic
TTAACAGATTAAGGAAGTTA+AGG 0.316539 2:+82030222 MsG0280011289.01.T01:CDS
TCCCCTCTTGAATTTGGAAA+TGG 0.323398 2:+82029999 MsG0280011289.01.T01:CDS
TACAATAGTGAGGAATTTCT+TGG 0.340204 2:+82031140 MsG0280011289.01.T01:CDS
CCAAGAAGATTCTGACTTCT+CGG 0.345962 2:+82030112 MsG0280011289.01.T01:CDS
CTTTGCGAAGAAAGCCGGTT+TGG 0.353761 2:+82030156 MsG0280011289.01.T01:CDS
TTCTAATGGTCCCATTAATT+CGG 0.354772 2:+82030537 MsG0280011289.01.T01:CDS
AGGTTATCAATGGTCTTGAT+AGG 0.358287 2:-82031176 None:intergenic
GGTCAGCTGCTTCTTGCTGC+TGG 0.365476 2:+82030577 MsG0280011289.01.T01:CDS
ACTTGTAGATGCAAACATTC+AGG 0.369324 2:+82030510 MsG0280011289.01.T01:CDS
TTTGAAAGAATCTAAGATTC+CGG 0.381602 2:-82030606 None:intergenic
CCCCTCTTGAATTTGGAAAT+GGG 0.395309 2:+82030000 MsG0280011289.01.T01:CDS
TGGACTAAAATCAATACAAC+GGG 0.404884 2:-82031351 None:intergenic
CTTCCTTAATCTGTTAACTT+TGG 0.410710 2:-82030216 None:intergenic
TTTGCGAAGAAAGCCGGTTT+GGG 0.414670 2:+82030157 MsG0280011289.01.T01:CDS
ATGGCTGTAGGAAACGCTTC+AGG 0.416776 2:+82031386 MsG0280011289.01.T01:CDS
TTCGAAAGGGGCAATCTTCA+AGG 0.417875 2:-82030665 None:intergenic
ACAAAATCAAAGCAATTAGT+TGG 0.426886 2:+82030894 MsG0280011289.01.T01:CDS
ACAATAGTGAGGAATTTCTT+GGG 0.430835 2:+82031141 MsG0280011289.01.T01:CDS
TGGAACTGAACCGAATTAAT+GGG 0.436701 2:-82030547 None:intergenic
GAATTAATGGGACCATTAGA+AGG 0.443632 2:-82030535 None:intergenic
TCGGTTCAGTTCCATCGAAA+TGG 0.445521 2:+82030556 MsG0280011289.01.T01:CDS
TTCAGTATTCAGGTTATCAA+TGG 0.445525 2:-82031186 None:intergenic
AAGTAATGTTGTTGCGAGCC+GGG 0.448192 2:+82030395 MsG0280011289.01.T01:CDS
AACATTCAGGATCCTTCTAA+TGG 0.448781 2:+82030523 MsG0280011289.01.T01:CDS
TTCTAAAATGATAGCCTTTG+TGG 0.449352 2:+82030842 MsG0280011289.01.T01:CDS
AAATATTAGCAATCTGTTCA+AGG 0.450551 2:+82031240 MsG0280011289.01.T01:CDS
TCTTCCTCATCCACCCAAAC+CGG 0.450796 2:-82030170 None:intergenic
TTTGCAGCTGGTTCGGCTAG+TGG 0.463183 2:+82031104 MsG0280011289.01.T01:CDS
CTTAGATTCTTTCAAATCGA+TGG 0.465769 2:+82030613 MsG0280011289.01.T01:CDS
GTAGATGAAGGCTGTTTAAA+CGG 0.466326 2:+82031047 MsG0280011289.01.T01:CDS
GCTGCAAAATGTGTCCCTAC+TGG 0.467974 2:-82031089 None:intergenic
CAGATTAAGGAAGTTAAGGA+AGG 0.470846 2:+82030226 MsG0280011289.01.T01:CDS
ACTGACCGTTCTGCTGATAG+TGG 0.477341 2:+82030077 MsG0280011289.01.T01:CDS
AAGCATCTTTCTTGCCCGAT+GGG 0.479466 2:+82030688 MsG0280011289.01.T01:CDS
GATGATGAATTGACAGATGA+TGG 0.480610 2:+82030372 MsG0280011289.01.T01:CDS
GAAGTAATGTTGTTGCGAGC+CGG 0.483388 2:+82030394 MsG0280011289.01.T01:CDS
GTGATGGCCATGTTTACCAT+TGG 0.483908 2:+82030991 MsG0280011289.01.T01:CDS
GGAAATGGGGATAACGATGA+AGG 0.486981 2:+82030014 MsG0280011289.01.T01:CDS
ACGGTCAGTGAAGAACAAAT+TGG 0.494840 2:-82030063 None:intergenic
TGATGGCCATGTTTACCATT+GGG 0.497623 2:+82030992 MsG0280011289.01.T01:CDS
TCTTTCAAATCGATGGCAAA+CGG 0.500797 2:+82030620 MsG0280011289.01.T01:CDS
AAGGAAGTTAAGGAAGGAAG+AGG 0.502686 2:+82030232 MsG0280011289.01.T01:CDS
GGAAGCCTTTGCAGCAGTCA+TGG 0.506331 2:-82030042 None:intergenic
TGATGCTTTGCGAAGAAAGC+CGG 0.516338 2:+82030151 MsG0280011289.01.T01:CDS
ATAGCCTTTGTGGGTGATGA+AGG 0.525756 2:+82030852 MsG0280011289.01.T01:CDS
CCCTACTGGAGAAGTACAAA+GGG 0.526256 2:-82031075 None:intergenic
AAGAGGACGAGAATGTGATC+TGG 0.530284 2:+82030249 MsG0280011289.01.T01:CDS
AAAGCCGGTTTGGGTGGATG+AGG 0.539602 2:+82030166 MsG0280011289.01.T01:CDS
TTTCTAATCTACTTTAGGCA+TGG 0.540589 2:-82031442 None:intergenic
CCCTCTTGAATTTGGAAATG+GGG 0.544010 2:+82030001 MsG0280011289.01.T01:CDS
ACTGGGCACAGCTTGATTCA+GGG 0.545660 2:+82030328 MsG0280011289.01.T01:intron
GGAAGCCATGACTGCTGCAA+AGG 0.547123 2:+82030037 MsG0280011289.01.T01:CDS
CCCCATTTCCAAATTCAAGA+GGG 0.548968 2:-82030001 None:intergenic
GTAAACATGGCCATCACCAC+CGG 0.549474 2:-82030985 None:intergenic
CAATACAACGGGGATAATGC+AGG 0.553209 2:-82031340 None:intergenic
TTCTTGCTGCTGGATTAGAC+CGG 0.554524 2:+82030587 MsG0280011289.01.T01:CDS
TGTGAATATTTACAATAGTG+AGG 0.554925 2:+82031130 MsG0280011289.01.T01:CDS
CAATAGTGAGGAATTTCTTG+GGG 0.554998 2:+82031142 MsG0280011289.01.T01:CDS
AAAGCATCTTTCTTGCCCGA+TGG 0.557386 2:+82030687 MsG0280011289.01.T01:CDS
GAAGATTCTGACTTCTCGGA+TGG 0.559324 2:+82030116 MsG0280011289.01.T01:CDS
TTGTTGAGCACCGGTGGTGA+TGG 0.564654 2:+82030975 MsG0280011289.01.T01:CDS
TCTAAAATGATAGCCTTTGT+GGG 0.566464 2:+82030843 MsG0280011289.01.T01:CDS
CCCTTTGTACTTCTCCAGTA+GGG 0.569388 2:+82031075 MsG0280011289.01.T01:CDS
GCGAAGAAAGCCGGTTTGGG+TGG 0.571477 2:+82030160 MsG0280011289.01.T01:CDS
GCCCTTTGTACTTCTCCAGT+AGG 0.573251 2:+82031074 MsG0280011289.01.T01:CDS
TGATAACCTGAATACTGAAG+TGG 0.580415 2:+82031190 MsG0280011289.01.T01:CDS
GGACAAAAGTTGTTGAGCAC+CGG 0.580640 2:+82030966 MsG0280011289.01.T01:CDS
ATTATCACTTGAGACCCATC+GGG 0.584332 2:-82030702 None:intergenic
TATTATCACTTGAGACCCAT+CGG 0.587218 2:-82030703 None:intergenic
GAAATGGGGATAACGATGAA+GGG 0.589801 2:+82030015 MsG0280011289.01.T01:CDS
CTTCAGTAAAAGCTAACGCA+CGG 0.597212 2:-82030941 None:intergenic
AGTAATGTTGTTGCGAGCCG+GGG 0.597573 2:+82030396 MsG0280011289.01.T01:CDS
GAAGCAGCTGACCATTTCGA+TGG 0.604108 2:-82030567 None:intergenic
TCCCCATTTCCAAATTCAAG+AGG 0.605102 2:-82030002 None:intergenic
TCAGATCCCAATGGTAAACA+TGG 0.606421 2:-82030998 None:intergenic
TTCAGTAAAAGCTAACGCAC+GGG 0.612384 2:-82030940 None:intergenic
TCCCTACTGGAGAAGTACAA+AGG 0.621550 2:-82031076 None:intergenic
CTGGACTAAAATCAATACAA+CGG 0.621707 2:-82031352 None:intergenic
ACCATTGGGATCTGAGAACA+AGG 0.624102 2:+82031006 MsG0280011289.01.T01:CDS
CCCATTTCCAAATTCAAGAG+GGG 0.625050 2:-82030000 None:intergenic
CCAGTTAGAGAAAACAGTGT+AGG 0.639938 2:-82031300 None:intergenic
GTTGGTACTCTGAAGATGAA+CGG 0.640176 2:+82030912 MsG0280011289.01.T01:CDS
TTAGAGAAAACAGTGTAGGA+TGG 0.640295 2:-82031296 None:intergenic
TGCAGGCTCGCATTTGAACG+CGG 0.645712 2:-82031323 None:intergenic
ACAGCCATAAAACCACCACC+TGG 0.646807 2:-82031371 None:intergenic
ATAACCTTCATCACCCACAA+AGG 0.647464 2:-82030856 None:intergenic
AAATGGGGATAACGATGAAG+GGG 0.648646 2:+82030016 MsG0280011289.01.T01:CDS
CAAAAGTTGTTGAGCACCGG+TGG 0.649375 2:+82030969 MsG0280011289.01.T01:CDS
AATAGTGAGGAATTTCTTGG+GGG 0.659821 2:+82031143 MsG0280011289.01.T01:CDS
ATGCACAAAGGTGTAGATGA+AGG 0.661644 2:+82031035 MsG0280011289.01.T01:CDS
AATCTTCTTGGTAAACAGTG+AGG 0.688434 2:-82030100 None:intergenic
TGAGGCCACTATCAGCAGAA+CGG 0.695939 2:-82030082 None:intergenic
ACAAGGACTTGTATGCACAA+AGG 0.714733 2:+82031023 MsG0280011289.01.T01:CDS
TCCTTGTTCTCAGATCCCAA+TGG 0.719029 2:-82031007 None:intergenic
GGACTAAAATCAATACAACG+GGG 0.796660 2:-82031350 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATAGTCTTGATTTAGTTA+AGG + Chr2:82030746-82030765 MsG0280011289.01.T01:intron 20.0%
! AAATATTAGCAATCTGTTCA+AGG + Chr2:82031240-82031259 MsG0280011289.01.T01:CDS 25.0%
! ACAAAATCAAAGCAATTAGT+TGG + Chr2:82030894-82030913 MsG0280011289.01.T01:CDS 25.0%
! TGTGAATATTTACAATAGTG+AGG + Chr2:82031130-82031149 MsG0280011289.01.T01:CDS 25.0%
! TTAACAGATTAAGGAAGTTA+AGG + Chr2:82030222-82030241 MsG0280011289.01.T01:CDS 25.0%
!! AAACTTGAAAGCTTTTTGTT+TGG + Chr2:82029966-82029985 MsG0280011289.01.T01:CDS 25.0%
!! TGATGAAGGTTATATTTTAC+TGG + Chr2:82030866-82030885 MsG0280011289.01.T01:CDS 25.0%
!! TTTGAAAGAATCTAAGATTC+CGG - Chr2:82030609-82030628 None:intergenic 25.0%
!!! AACTTGAAAGCTTTTTGTTT+GGG + Chr2:82029967-82029986 MsG0280011289.01.T01:CDS 25.0%
ACAATAGTGAGGAATTTCTT+GGG + Chr2:82031141-82031160 MsG0280011289.01.T01:CDS 30.0%
CTGGACTAAAATCAATACAA+CGG - Chr2:82031355-82031374 None:intergenic 30.0%
CTTAGATTCTTTCAAATCGA+TGG + Chr2:82030613-82030632 MsG0280011289.01.T01:CDS 30.0%
CTTCCTTAATCTGTTAACTT+TGG - Chr2:82030219-82030238 None:intergenic 30.0%
TACAATAGTGAGGAATTTCT+TGG + Chr2:82031140-82031159 MsG0280011289.01.T01:CDS 30.0%
TAGCCAAAGTTAACAGATTA+AGG + Chr2:82030213-82030232 MsG0280011289.01.T01:CDS 30.0%
TGGACTAAAATCAATACAAC+GGG - Chr2:82031354-82031373 None:intergenic 30.0%
TTCTAATGGTCCCATTAATT+CGG + Chr2:82030537-82030556 MsG0280011289.01.T01:CDS 30.0%
! AAGAAAGATGCTTTTCGAAA+GGG - Chr2:82030681-82030700 None:intergenic 30.0%
! TCTAAAATGATAGCCTTTGT+GGG + Chr2:82030843-82030862 MsG0280011289.01.T01:CDS 30.0%
! TTCAGTATTCAGGTTATCAA+TGG - Chr2:82031189-82031208 None:intergenic 30.0%
!! AAGGCTAGAGTTGATAAAAT+TGG + Chr2:82030765-82030784 MsG0280011289.01.T01:intron 30.0%
!! TTCTAAAATGATAGCCTTTG+TGG + Chr2:82030842-82030861 MsG0280011289.01.T01:CDS 30.0%
!!! AAGGCTATCATTTTAGAATC+AGG - Chr2:82030840-82030859 None:intergenic 30.0%
!!! GAAGAGTATTGAAGTTTTTG+AGG + Chr2:82030809-82030828 MsG0280011289.01.T01:CDS 30.0%
AACATTCAGGATCCTTCTAA+TGG + Chr2:82030523-82030542 MsG0280011289.01.T01:CDS 35.0%
AATAGTGAGGAATTTCTTGG+GGG + Chr2:82031143-82031162 MsG0280011289.01.T01:CDS 35.0%
AATCTTCTTGGTAAACAGTG+AGG - Chr2:82030103-82030122 None:intergenic 35.0%
ACTTGTAGATGCAAACATTC+AGG + Chr2:82030510-82030529 MsG0280011289.01.T01:CDS 35.0%
AGAGTATGTGTCAAGATTGA+GGG + Chr2:82030275-82030294 MsG0280011289.01.T01:intron 35.0%
ATGGAACTGAACCGAATTAA+TGG - Chr2:82030551-82030570 None:intergenic 35.0%
CAATAGTGAGGAATTTCTTG+GGG + Chr2:82031142-82031161 MsG0280011289.01.T01:CDS 35.0%
CAGATTAAGGAAGTTAAGGA+AGG + Chr2:82030226-82030245 MsG0280011289.01.T01:CDS 35.0%
GAATTAATGGGACCATTAGA+AGG - Chr2:82030538-82030557 None:intergenic 35.0%
GATGATGAATTGACAGATGA+TGG + Chr2:82030372-82030391 MsG0280011289.01.T01:CDS 35.0%
GGACTAAAATCAATACAACG+GGG - Chr2:82031353-82031372 None:intergenic 35.0%
GTAGATGAAGGCTGTTTAAA+CGG + Chr2:82031047-82031066 MsG0280011289.01.T01:CDS 35.0%
TATTATCACTTGAGACCCAT+CGG - Chr2:82030706-82030725 None:intergenic 35.0%
TCTCAGAACAAATGAAGACT+TGG + Chr2:82030440-82030459 MsG0280011289.01.T01:intron 35.0%
TCTTTCAAATCGATGGCAAA+CGG + Chr2:82030620-82030639 MsG0280011289.01.T01:CDS 35.0%
TGAAATCCACTTCAGTATTC+AGG - Chr2:82031199-82031218 None:intergenic 35.0%
TGATAACCTGAATACTGAAG+TGG + Chr2:82031190-82031209 MsG0280011289.01.T01:CDS 35.0%
TGGAACTGAACCGAATTAAT+GGG - Chr2:82030550-82030569 None:intergenic 35.0%
TTAGAGAAAACAGTGTAGGA+TGG - Chr2:82031299-82031318 None:intergenic 35.0%
TTCGAGTATTCAATATGACC+TGG - Chr2:82030490-82030509 None:intergenic 35.0%
! AACAAATGAAGACTTGGTTG+TGG + Chr2:82030446-82030465 MsG0280011289.01.T01:intron 35.0%
! AGAAAGATGCTTTTCGAAAG+GGG - Chr2:82030680-82030699 None:intergenic 35.0%
! AGGTTATCAATGGTCTTGAT+AGG - Chr2:82031179-82031198 None:intergenic 35.0%
! CAAGAAAGATGCTTTTCGAA+AGG - Chr2:82030682-82030701 None:intergenic 35.0%
! CAAGGAAGATGCTTTGAATT+TGG - Chr2:82030650-82030669 None:intergenic 35.0%
!!! CGTTGTATTGATTTTAGTCC+AGG + Chr2:82031353-82031372 MsG0280011289.01.T01:CDS 35.0%
!!! TGTATTGATTTTAGTCCAGG+TGG + Chr2:82031356-82031375 MsG0280011289.01.T01:CDS 35.0%
AAATGGGGATAACGATGAAG+GGG + Chr2:82030016-82030035 MsG0280011289.01.T01:CDS 40.0%
AAGGAAGTTAAGGAAGGAAG+AGG + Chr2:82030232-82030251 MsG0280011289.01.T01:CDS 40.0%
ACAAGGACTTGTATGCACAA+AGG + Chr2:82031023-82031042 MsG0280011289.01.T01:CDS 40.0%
ACGGTCAGTGAAGAACAAAT+TGG - Chr2:82030066-82030085 None:intergenic 40.0%
ATAACCTTCATCACCCACAA+AGG - Chr2:82030859-82030878 None:intergenic 40.0%
ATTATCACTTGAGACCCATC+GGG - Chr2:82030705-82030724 None:intergenic 40.0%
CAGAGTATGTGTCAAGATTG+AGG + Chr2:82030274-82030293 MsG0280011289.01.T01:intron 40.0%
CCAAGAAGATTCTGACTTCT+CGG + Chr2:82030112-82030131 MsG0280011289.01.T01:CDS 40.0%
CCAGTTAGAGAAAACAGTGT+AGG - Chr2:82031303-82031322 None:intergenic 40.0%
CCCATTTCCAAATTCAAGAG+GGG - Chr2:82030003-82030022 None:intergenic 40.0%
CCCCATTTCCAAATTCAAGA+GGG - Chr2:82030004-82030023 None:intergenic 40.0%
CCCCTCTTGAATTTGGAAAT+GGG + Chr2:82030000-82030019 MsG0280011289.01.T01:CDS 40.0%
CCCTCTTGAATTTGGAAATG+GGG + Chr2:82030001-82030020 MsG0280011289.01.T01:CDS 40.0%
CTTCAGTAAAAGCTAACGCA+CGG - Chr2:82030944-82030963 None:intergenic 40.0%
GAAATGGGGATAACGATGAA+GGG + Chr2:82030015-82030034 MsG0280011289.01.T01:CDS 40.0%
GATTCAGGGTCAAAAATGGA+TGG + Chr2:82030342-82030361 MsG0280011289.01.T01:CDS 40.0%
GCTTGATTCAGGGTCAAAAA+TGG + Chr2:82030338-82030357 MsG0280011289.01.T01:intron 40.0%
GGGTCTCAAGTGATAATATC+TGG + Chr2:82030708-82030727 MsG0280011289.01.T01:intron 40.0%
TCAGATCCCAATGGTAAACA+TGG - Chr2:82031001-82031020 None:intergenic 40.0%
TCCCCATTTCCAAATTCAAG+AGG - Chr2:82030005-82030024 None:intergenic 40.0%
TCCCCTCTTGAATTTGGAAA+TGG + Chr2:82029999-82030018 MsG0280011289.01.T01:CDS 40.0%
TGATAAAATTGGTCCGCTGT+TGG + Chr2:82030776-82030795 MsG0280011289.01.T01:intron 40.0%
TGATGGCCATGTTTACCATT+GGG + Chr2:82030992-82031011 MsG0280011289.01.T01:CDS 40.0%
TTCAGTAAAAGCTAACGCAC+GGG - Chr2:82030943-82030962 None:intergenic 40.0%
! ATGCACAAAGGTGTAGATGA+AGG + Chr2:82031035-82031054 MsG0280011289.01.T01:CDS 40.0%
! CCTACACTGTTTTCTCTAAC+TGG + Chr2:82031300-82031319 MsG0280011289.01.T01:CDS 40.0%
! GTTGGTACTCTGAAGATGAA+CGG + Chr2:82030912-82030931 MsG0280011289.01.T01:CDS 40.0%
!! ATTGATTTTAGTCCAGGTGG+TGG + Chr2:82031359-82031378 MsG0280011289.01.T01:CDS 40.0%
!!! GCGTTAGCTTTTACTGAAGA+TGG + Chr2:82030945-82030964 MsG0280011289.01.T01:CDS 40.0%
AAATTGGTCCGCTGTTGGAT+AGG + Chr2:82030781-82030800 MsG0280011289.01.T01:intron 45.0%
AAGAGGACGAGAATGTGATC+TGG + Chr2:82030249-82030268 MsG0280011289.01.T01:CDS 45.0%
AAGCATCTTTCTTGCCCGAT+GGG + Chr2:82030688-82030707 MsG0280011289.01.T01:CDS 45.0%
AAGTAATGTTGTTGCGAGCC+GGG + Chr2:82030395-82030414 MsG0280011289.01.T01:CDS 45.0%
AATTGGTCCGCTGTTGGATA+GGG + Chr2:82030782-82030801 MsG0280011289.01.T01:intron 45.0%
ACCATTGGGATCTGAGAACA+AGG + Chr2:82031006-82031025 MsG0280011289.01.T01:CDS 45.0%
AGCAGCTCAAAATTGTTGCC+AGG + Chr2:82030469-82030488 MsG0280011289.01.T01:intron 45.0%
ATAGCCTTTGTGGGTGATGA+AGG + Chr2:82030852-82030871 MsG0280011289.01.T01:CDS 45.0%
CAATACAACGGGGATAATGC+AGG - Chr2:82031343-82031362 None:intergenic 45.0%
CCCTTTGTACTTCTCCAGTA+GGG + Chr2:82031075-82031094 MsG0280011289.01.T01:CDS 45.0%
CCGAGAAGTCAGAATCTTCT+TGG - Chr2:82030115-82030134 None:intergenic 45.0%
CTCTACTCCCCTCTTGAATT+TGG + Chr2:82029993-82030012 MsG0280011289.01.T01:CDS 45.0%
GAAGATTCTGACTTCTCGGA+TGG + Chr2:82030116-82030135 MsG0280011289.01.T01:CDS 45.0%
GAAGTAATGTTGTTGCGAGC+CGG + Chr2:82030394-82030413 MsG0280011289.01.T01:CDS 45.0%
GGAAATGGGGATAACGATGA+AGG + Chr2:82030014-82030033 MsG0280011289.01.T01:CDS 45.0%
GGACAAAAGTTGTTGAGCAC+CGG + Chr2:82030966-82030985 MsG0280011289.01.T01:CDS 45.0%
GTGATGGCCATGTTTACCAT+TGG + Chr2:82030991-82031010 MsG0280011289.01.T01:CDS 45.0%
TCCTTGTTCTCAGATCCCAA+TGG - Chr2:82031010-82031029 None:intergenic 45.0%
TCGGTTCAGTTCCATCGAAA+TGG + Chr2:82030556-82030575 MsG0280011289.01.T01:CDS 45.0%
TGATGCTTTGCGAAGAAAGC+CGG + Chr2:82030151-82030170 MsG0280011289.01.T01:CDS 45.0%
TTCGAAAGGGGCAATCTTCA+AGG - Chr2:82030668-82030687 None:intergenic 45.0%
TTCTCTTCCCTATCCAACAG+CGG - Chr2:82030792-82030811 None:intergenic 45.0%
! AAAGCATCTTTCTTGCCCGA+TGG + Chr2:82030687-82030706 MsG0280011289.01.T01:CDS 45.0%
! CCCTACTGGAGAAGTACAAA+GGG - Chr2:82031078-82031097 None:intergenic 45.0%
! GACACATTTTGCAGCTGGTT+CGG + Chr2:82031097-82031116 MsG0280011289.01.T01:CDS 45.0%
! TCCCTACTGGAGAAGTACAA+AGG - Chr2:82031079-82031098 None:intergenic 45.0%
! TTCTTGCTGCTGGATTAGAC+CGG + Chr2:82030587-82030606 MsG0280011289.01.T01:CDS 45.0%
! TTTGCGAAGAAAGCCGGTTT+GGG + Chr2:82030157-82030176 MsG0280011289.01.T01:CDS 45.0%
!! TAGTCCAGGTGGTGGTTTTA+TGG + Chr2:82031367-82031386 MsG0280011289.01.T01:CDS 45.0%
ACAGCCATAAAACCACCACC+TGG - Chr2:82031374-82031393 None:intergenic 50.0%
ACTGACCGTTCTGCTGATAG+TGG + Chr2:82030077-82030096 MsG0280011289.01.T01:CDS 50.0%
ACTGGGCACAGCTTGATTCA+GGG + Chr2:82030328-82030347 MsG0280011289.01.T01:intron 50.0%
AGTAATGTTGTTGCGAGCCG+GGG + Chr2:82030396-82030415 MsG0280011289.01.T01:CDS 50.0%
ATGGCTGTAGGAAACGCTTC+AGG + Chr2:82031386-82031405 MsG0280011289.01.T01:CDS 50.0%
CAACATGTGAAGCTCAACCG+AGG + Chr2:82030300-82030319 MsG0280011289.01.T01:intron 50.0%
CATCCAGATCCTCATAACCC+CGG - Chr2:82030416-82030435 None:intergenic 50.0%
CTTTGCGAAGAAAGCCGGTT+TGG + Chr2:82030156-82030175 MsG0280011289.01.T01:CDS 50.0%
GAAGCAGCTGACCATTTCGA+TGG - Chr2:82030570-82030589 None:intergenic 50.0%
GCCCTTTGTACTTCTCCAGT+AGG + Chr2:82031074-82031093 MsG0280011289.01.T01:CDS 50.0%
GCTGCAAAATGTGTCCCTAC+TGG - Chr2:82031092-82031111 None:intergenic 50.0%
GTAAACATGGCCATCACCAC+CGG - Chr2:82030988-82031007 None:intergenic 50.0%
TCTTCCTCATCCACCCAAAC+CGG - Chr2:82030173-82030192 None:intergenic 50.0%
TGAGGCCACTATCAGCAGAA+CGG - Chr2:82030085-82030104 None:intergenic 50.0%
! GTAGGGACACATTTTGCAGC+TGG + Chr2:82031092-82031111 MsG0280011289.01.T01:CDS 50.0%
!! CAAAAGTTGTTGAGCACCGG+TGG + Chr2:82030969-82030988 MsG0280011289.01.T01:CDS 50.0%
!!! GGTGGTGGTTTTATGGCTGT+AGG + Chr2:82031374-82031393 MsG0280011289.01.T01:CDS 50.0%
AGCTCAACCGAGGAACTGAC+TGG + Chr2:82030310-82030329 MsG0280011289.01.T01:intron 55.0%
GACTGGGCACAGCTTGATTC+AGG + Chr2:82030327-82030346 MsG0280011289.01.T01:intron 55.0%
GCTCAACCGAGGAACTGACT+GGG + Chr2:82030311-82030330 MsG0280011289.01.T01:intron 55.0%
GGAAGCCATGACTGCTGCAA+AGG + Chr2:82030037-82030056 MsG0280011289.01.T01:CDS 55.0%
GGAAGCCTTTGCAGCAGTCA+TGG - Chr2:82030045-82030064 None:intergenic 55.0%
TGCAGGCTCGCATTTGAACG+CGG - Chr2:82031326-82031345 None:intergenic 55.0%
!! AAAGCCGGTTTGGGTGGATG+AGG + Chr2:82030166-82030185 MsG0280011289.01.T01:CDS 55.0%
!! TTGTTGAGCACCGGTGGTGA+TGG + Chr2:82030975-82030994 MsG0280011289.01.T01:CDS 55.0%
!! TTTGCAGCTGGTTCGGCTAG+TGG + Chr2:82031104-82031123 MsG0280011289.01.T01:CDS 55.0%
GAGCCGGGGTTATGAGGATC+TGG + Chr2:82030410-82030429 MsG0280011289.01.T01:intron 60.0%
GCTGTGCCCAGTCAGTTCCT+CGG - Chr2:82030320-82030339 None:intergenic 60.0%
GGTCAGCTGCTTCTTGCTGC+TGG + Chr2:82030577-82030596 MsG0280011289.01.T01:CDS 60.0%
TGTTGCGAGCCGGGGTTATG+AGG + Chr2:82030404-82030423 MsG0280011289.01.T01:intron 60.0%
!! GCGAAGAAAGCCGGTTTGGG+TGG + Chr2:82030160-82030179 MsG0280011289.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 82029960 82031451 82029960 ID=MsG0280011289.01;Name=MsG0280011289.01
Chr2 mRNA 82029960 82031451 82029960 ID=MsG0280011289.01.T01;Parent=MsG0280011289.01;Name=MsG0280011289.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|4|0|423
Chr2 exon 82029960 82030277 82029960 ID=MsG0280011289.01.T01:exon:12198;Parent=MsG0280011289.01.T01
Chr2 exon 82030339 82030417 82030339 ID=MsG0280011289.01.T01:exon:12199;Parent=MsG0280011289.01.T01
Chr2 exon 82030491 82030716 82030491 ID=MsG0280011289.01.T01:exon:12200;Parent=MsG0280011289.01.T01
Chr2 exon 82030803 82031451 82030803 ID=MsG0280011289.01.T01:exon:12201;Parent=MsG0280011289.01.T01
Chr2 CDS 82029960 82030277 82029960 ID=MsG0280011289.01.T01:cds;Parent=MsG0280011289.01.T01
Chr2 CDS 82030339 82030417 82030339 ID=MsG0280011289.01.T01:cds;Parent=MsG0280011289.01.T01
Chr2 CDS 82030491 82030716 82030491 ID=MsG0280011289.01.T01:cds;Parent=MsG0280011289.01.T01
Chr2 CDS 82030803 82031451 82030803 ID=MsG0280011289.01.T01:cds;Parent=MsG0280011289.01.T01
Gene Sequence

>MsG0280011289.01.T01

ATGAAAAAACTTGAAAGCTTTTTGTTTGGGTCTCTCTACTCCCCTCTTGAATTTGGAAATGGGGATAACGATGAAGGGGAAGCCATGACTGCTGCAAAGGCTTCCAATTTGTTCTTCACTGACCGTTCTGCTGATAGTGGCCTCACTGTTTACCAAGAAGATTCTGACTTCTCGGATGGAAGTTGTGATGATGATGCTTTGCGAAGAAAGCCGGTTTGGGTGGATGAGGAAGAAGAGAAAGTCACTGTTAACATAGCCAAAGTTAACAGATTAAGGAAGTTAAGGAAGGAAGAGGACGAGAATGTGATCTGGTTCAGACTTGATTCAGGGTCAAAAATGGATGGATCTTCTGATGATGAATTGACAGATGATGGAAGTAATGTTGTTGCGAGCCGGGGTCATATTGAATACTCGAAACTTGTAGATGCAAACATTCAGGATCCTTCTAATGGTCCCATTAATTCGGTTCAGTTCCATCGAAATGGTCAGCTGCTTCTTGCTGCTGGATTAGACCGGAATCTTAGATTCTTTCAAATCGATGGCAAACGGAACACCAAATTCAAAGCATCTTCCTTGAAGATTGCCCCTTTCGAAAAGCATCTTTCTTGCCCGATGGGTCTCAAGGAAGAGAAGAGTATTGAAGTTTTTGAGGTCTCTCCTGATTCTAAAATGATAGCCTTTGTGGGTGATGAAGGTTATATTTTACTGGTTTCAACAAAATCAAAGCAATTAGTTGGTACTCTGAAGATGAACGGAACAGCCCGTGCGTTAGCTTTTACTGAAGATGGACAAAAGTTGTTGAGCACCGGTGGTGATGGCCATGTTTACCATTGGGATCTGAGAACAAGGACTTGTATGCACAAAGGTGTAGATGAAGGCTGTTTAAACGGCACAGCCCTTTGTACTTCTCCAGTAGGGACACATTTTGCAGCTGGTTCGGCTAGTGGAGTTGTGAATATTTACAATAGTGAGGAATTTCTTGGGGGAAAGAGAAAACCTATCAAGACCATTGATAACCTGAATACTGAAGTGGATTTCATGAAATTTAATCATGACTCTCAAATATTAGCAATCTGTTCAAGGTCAAAGAATAGTAGTTTGAAATTAATACATATACCATCCTACACTGTTTTCTCTAACTGGCCGCGTTCAAATGCGAGCCTGCATTATCCCCGTTGTATTGATTTTAGTCCAGGTGGTGGTTTTATGGCTGTAGGAAACGCTTCAGGCAAAGTGTTACTATATAAGTTGCATCATTATCACCATGCCTAA
Protein sequence

>MsG0280011289.01.T01

MKKLESFLFGSLYSPLEFGNGDNDEGEAMTAAKASNLFFTDRSADSGLTVYQEDSDFSDGSCDDDALRRKPVWVDEEEEKVTVNIAKVNRLRKLRKEEDENVIWFRLDSGSKMDGSSDDELTDDGSNVVASRGHIEYSKLVDANIQDPSNGPINSVQFHRNGQLLLAAGLDRNLRFFQIDGKRNTKFKASSLKIAPFEKHLSCPMGLKEEKSIEVFEVSPDSKMIAFVGDEGYILLVSTKSKQLVGTLKMNGTARALAFTEDGQKLLSTGGDGHVYHWDLRTRTCMHKGVDEGCLNGTALCTSPVGTHFAAGSASGVVNIYNSEEFLGGKRKPIKTIDNLNTEVDFMKFNHDSQILAICSRSKNSSLKLIHIPSYTVFSNWPRSNASLHYPRCIDFSPGGGFMAVGNASGKVLLYKLHHYHHA*