AlfalfaGEDB | MsG0280011369.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011369.01

KEGG: -
GO: -
PFAM: PF04862

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011369.01.T01	XP_003597843.1	98.857	350	4	0	5	354	24	373	0	715

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011369.01.T01	G7IMH9	98.857	350	4	0	5	354	24	373	0.0	715

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005569.01	MsG0280011369.01	0.800086	1.699317e-48	7.842286e-46
MsG0280011369.01	MsG0380011846.01	0.821128	4.759832e-53	3.839262e-50
MsG0280011369.01	MsG0380012818.01	0.831668	1.465229e-55	1.600950e-52
MsG0280011369.01	MsG0380017079.01	0.847432	1.160811e-59	2.062940e-56
MsG0280011369.01	MsG0480021277.01	0.848958	4.397408e-60	8.208073e-57
MsG0280011369.01	MsG0780040990.01	0.801746	7.786338e-49	3.747400e-46
MsG0280011369.01	MsG0880043483.01	0.842730	2.162715e-58	3.308870e-55
MsG0280011369.01	MsG0880046858.01	0.805626	1.219289e-49	6.481910e-47

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PPI

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Gene1	Gene2	Type
MsG0280011369.01	MsG0480019817.01	PPI
MsG0380012818.01	MsG0280011369.01	PPI
MsG0480019818.01	MsG0280011369.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011369.01.T01	MTR_2g103170	98.857	350	4	0	5	354	24	373	0.0	715
MsG0280011369.01.T01	MTR_1g011800	68.876	347	102	2	5	350	35	376	3.78e-180	504
MsG0280011369.01.T01	MTR_0045s0110	50.595	336	160	2	7	342	32	361	3.99e-119	349
MsG0280011369.01.T01	MTR_4g039740	50.599	334	159	2	9	342	34	361	2.77e-115	339
MsG0280011369.01.T01	MTR_4g039720	50.000	334	161	2	9	342	38	365	1.07e-114	338
MsG0280011369.01.T01	MTR_2g019600	46.647	343	178	1	2	344	28	365	2.46e-111	330
MsG0280011369.01.T01	MTR_4g039680	47.904	334	168	2	9	342	34	361	2.49e-110	327

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011369.01.T01	AT5G11420	67.341	346	108	1	5	350	26	366	7.15e-179	500
MsG0280011369.01.T01	AT5G25460	67.919	346	106	1	5	350	29	369	6.51e-178	498
MsG0280011369.01.T01	AT4G32460	67.630	346	107	1	5	350	25	365	4.93e-175	491
MsG0280011369.01.T01	AT4G32460	67.630	346	107	1	5	350	25	365	4.93e-175	491
MsG0280011369.01.T01	AT1G80240	66.472	343	110	1	5	347	28	365	1.55e-168	474
MsG0280011369.01.T01	AT4G32460	67.273	330	103	1	21	350	1	325	1.91e-165	465
MsG0280011369.01.T01	AT4G32460	67.273	330	103	1	21	350	1	325	1.91e-165	465
MsG0280011369.01.T01	AT3G08030	52.786	341	156	1	2	342	24	359	9.91e-123	358
MsG0280011369.01.T01	AT2G41800	49.709	344	168	1	5	348	32	370	2.56e-119	349
MsG0280011369.01.T01	AT3G08030	53.106	322	146	1	21	342	1	317	1.17e-116	341

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Find 92 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAGGTGACATGCTTT+TGG	0.342997	2:+83142398	MsG0280011369.01.T01:CDS
AAAAGGTGAAATTAAATAAA+GGG	0.413489	2:+83142475	MsG0280011369.01.T01:CDS
AAATTACCTCTGGTTCTTCT+AGG	0.274083	2:-83142765	None:intergenic
AACCCACCTAGAAGAACCAG+AGG	0.693541	2:+83142759	MsG0280011369.01.T01:CDS
AAGGCAACAGAATCAATAAG+TGG	0.552750	2:-83142726	None:intergenic
AATACTCCCCATGATGCATT+TGG	0.357606	2:-83143361	None:intergenic
ACCAGAGTTATCATTCTTCA+TGG	0.360617	2:-83143761	None:intergenic
ACCATGAAGAATGATAACTC+TGG	0.691442	2:+83143760	MsG0280011369.01.T01:CDS
ACTTCTGAGAAAAGAGATTA+TGG	0.344578	2:+83142573	MsG0280011369.01.T01:CDS
AGAGAAGCTTAATGTGTCAG+TGG	0.619453	2:+83142545	MsG0280011369.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGTTGCAAATCAAAATGCA+TGG	+	Chr2:83143856-83143875	MsG0280011369.01.T01:three_prime_UTR	30.0%
ATTACTTAGGTATGCTTGTA+GGG	-	Chr2:83143838-83143857	None:intergenic	30.0%
ATTCCAATACAGACTATGTA+TGG	+	Chr2:83142600-83142619	MsG0280011369.01.T01:CDS	30.0%
CAAGTTCCTTATCAATCTAA+GGG	+	Chr2:83143655-83143674	MsG0280011369.01.T01:CDS	30.0%
CTTGTAATAATATAGCTAGG+AGG	+	Chr2:83143932-83143951	MsG0280011369.01.T01:three_prime_UTR	30.0%
GAAATTGTTATCCATAACTC+TGG	+	Chr2:83142678-83142697	MsG0280011369.01.T01:CDS	30.0%
TCAAGTTCCTTATCAATCTA+AGG	+	Chr2:83143654-83143673	MsG0280011369.01.T01:CDS	30.0%
TCATGGTGTAAAATGTACTT+AGG	-	Chr2:83143747-83143766	None:intergenic	30.0%
TTATGATGTGTTTGGATTGA+CGG	+	Chr2:83143034-83143053	MsG0280011369.01.T01:intron	30.0%
AAGGCAACAGAATCAATAAG+TGG	-	Chr2:83142729-83142748	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	83141971	83144083	83141971	ID=MsG0280011369.01;Name=MsG0280011369.01
Chr2	mRNA	83141971	83144083	83141971	ID=MsG0280011369.01.T01;Parent=MsG0280011369.01;Name=MsG0280011369.01.T01;_AED=0.47;_eAED=0.48;_QI=0\|0.5\|0.33\|1\|1\|1\|3\|231\|354
Chr2	exon	83141971	83141983	83141971	ID=MsG0280011369.01.T01:exon:12652;Parent=MsG0280011369.01.T01
Chr2	exon	83142268	83142780	83142268	ID=MsG0280011369.01.T01:exon:12653;Parent=MsG0280011369.01.T01
Chr2	exon	83143314	83144083	83143314	ID=MsG0280011369.01.T01:exon:12654;Parent=MsG0280011369.01.T01
Chr2	CDS	83141971	83141983	83141971	ID=MsG0280011369.01.T01:cds;Parent=MsG0280011369.01.T01
Chr2	CDS	83142268	83142780	83142268	ID=MsG0280011369.01.T01:cds;Parent=MsG0280011369.01.T01
Chr2	CDS	83143314	83143852	83143314	ID=MsG0280011369.01.T01:cds;Parent=MsG0280011369.01.T01
Chr2	three_prime_UTR	83143853	83144083	83143853	ID=MsG0280011369.01.T01:three_prime_utr;Parent=MsG0280011369.01.T01

Gene Sequence

>MsG0280011369.01.T01

ATGGCTTACAAAGGTTTATTGCCAAATGGTGACTTTGAAGTTGGTCCAAAAGCATCAAACCTAAAGGGCACAGTAGTGACAACCCATGATGCCATACCTAATTGGACAGTTTCAGGATACGTTGAATACATAAAATCAGGACAAAAACAAGGTGACATGCTTTTGGTTGTACCAGAAGGTGCTTATGCTGTTAGGCTAGGAAATGAAGCATACATTAAACAAAAGGTGAAATTAAATAAAGGGTCATCTTATTCCATCACGTTCAGTGCAGCTCGCACGTGTGCACAAGAAGAGAAGCTTAATGTGTCAGTGGTACCAACTTCTGAGAAAAGAGATTATGGTATCATTCCAATACAGACTATGTATGGTAGCAATGGTTGGGAATCATTTGCTTGTGGGTTTAGAGCTGATTATCCAGAAGGGGAAATTGTTATCCATAACTCTGGTGTTGAAGATGATCCAGCTTGTGGCCCACTTATTGATTCTGTTGCCTTGAAGGTTTTGAACCCACCTAGAAGAACCAGAGCTAATTTATTGAAAAATGGAAACTTTGAAGAAGGACCATATGTGTTCCCAAATGCATCATGGGGAGTATTGATCCCACCACACATTGAGGATGCTCATGGTCCATTACCAGGATGGATAGTTGAATCTCTCAAAGCAGTGAAATACATTGATTCTGAACACTTCACAGTACCAGAAGGAAAAAGAGCAATTGAACTTGTAGCAGGGAAAGAAAGTGCACTTGCACAAGAAGTCTTCACCACAATTGGAAAAGTTTATGTCCTCACTTTTGCAGTTGGTGATGCTAATAATGCATGTGAAGGTTCTATGACTGTAGAAGCATTTGCTGGTAGAGACACTGTTCAAGTTCCTTATCAATCTAAGGGCAAAGGAGGGTTTGTTAGAGGCAAACTTCGTTTTAAGGCTACGACAAGACGTACTAGGATTAGGTTCCTAAGTACATTTTACACCATGAAGAATGATAACTCTGGTTCTCTTTGTGGACCTGTTATTGATGATGTAAAATTGCTTAGTGTTCGTTATCCCTACAAGCATACCTAA

Protein sequence

>MsG0280011369.01.T01

MAYKGLLPNGDFEVGPKASNLKGTVVTTHDAIPNWTVSGYVEYIKSGQKQGDMLLVVPEGAYAVRLGNEAYIKQKVKLNKGSSYSITFSAARTCAQEEKLNVSVVPTSEKRDYGIIPIQTMYGSNGWESFACGFRADYPEGEIVIHNSGVEDDPACGPLIDSVALKVLNPPRRTRANLLKNGNFEEGPYVFPNASWGVLIPPHIEDAHGPLPGWIVESLKAVKYIDSEHFTVPEGKRAIELVAGKESALAQEVFTTIGKVYVLTFAVGDANNACEGSMTVEAFAGRDTVQVPYQSKGKGGFVRGKLRFKATTRRTRIRFLSTFYTMKNDNSGSLCGPVIDDVKLLSVRYPYKHT*