AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021277.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021277.01.T01 MTR_4g070570 97.000 300 9 0 2 301 4 303 0.0 591
MsG0480021277.01.T01 MTR_4g070590 58.333 300 113 6 8 301 8 301 2.13e-110 322
MsG0480021277.01.T01 MTR_3g116950 55.814 301 119 7 6 301 5 296 6.03e-102 300
MsG0480021277.01.T01 MTR_3g116920 56.333 300 121 6 6 301 5 298 2.40e-99 294
MsG0480021277.01.T01 MTR_4g070540 43.253 289 154 4 16 301 15 296 7.57e-63 201
MsG0480021277.01.T01 MTR_4g070530 41.176 289 160 4 16 301 15 296 1.06e-59 192
MsG0480021277.01.T01 MTR_8g074530 34.576 295 182 6 10 300 17 304 1.37e-46 159
MsG0480021277.01.T01 MTR_5g023550 31.525 295 185 8 11 301 3 284 4.70e-39 139
MsG0480021277.01.T01 MTR_8g074510 29.655 290 195 5 13 300 5 287 1.02e-38 138
MsG0480021277.01.T01 MTR_8g074450 32.646 291 185 6 13 299 5 288 1.09e-38 138
MsG0480021277.01.T01 MTR_5g023290 30.573 314 198 9 1 300 1 308 8.09e-38 136
MsG0480021277.01.T01 MTR_5g023390 30.847 295 187 8 9 299 2 283 1.74e-37 135
MsG0480021277.01.T01 MTR_5g023600 31.081 296 185 9 9 299 2 283 6.24e-37 133
MsG0480021277.01.T01 MTR_5g023370 30.847 295 187 8 9 299 2 283 8.38e-37 133
MsG0480021277.01.T01 MTR_8g074420 30.479 292 192 6 13 300 5 289 8.46e-37 133
MsG0480021277.01.T01 MTR_5g023340 30.612 294 189 6 9 299 2 283 1.07e-36 132
MsG0480021277.01.T01 MTR_5g023350 29.831 295 190 8 9 299 2 283 1.72e-35 129
MsG0480021277.01.T01 MTR_5g023520 30.872 298 184 11 11 301 3 285 1.74e-35 129
MsG0480021277.01.T01 MTR_5g023530 30.743 296 190 8 7 299 1 284 2.44e-35 129
MsG0480021277.01.T01 MTR_5g023470 30.743 296 190 8 7 299 1 284 1.76e-34 127
MsG0480021277.01.T01 MTR_5g023320 29.966 297 185 10 9 299 2 281 3.09e-34 126
MsG0480021277.01.T01 MTR_5g023580 29.592 294 192 7 10 298 3 286 5.78e-34 127
MsG0480021277.01.T01 MTR_8g074380 31.475 305 174 9 4 299 3 281 7.85e-27 107
MsG0480021277.01.T01 MTR_3g116940 43.307 127 46 1 1 127 1 101 4.20e-24 95.9
MsG0480021277.01.T01 MTR_5g023650 30.519 308 189 7 9 301 8 305 6.96e-23 96.3
MsG0480021277.01.T01 MTR_1g037820 32.900 231 126 8 72 301 24 226 8.71e-21 89.4
MsG0480021277.01.T01 MTR_5g023460 31.633 196 117 6 105 299 11 190 5.66e-19 83.2
MsG0480021277.01.T01 MTR_3g115150 26.144 306 197 11 9 299 23 314 5.62e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021277.01.T01 AT2G17080 40.625 288 137 9 16 301 8 263 5.64e-51 169
MsG0480021277.01.T01 AT2G17070 38.889 288 142 8 16 301 8 263 7.79e-51 169
MsG0480021277.01.T01 AT4G35210 39.576 283 131 9 16 298 8 250 1.36e-48 162
MsG0480021277.01.T01 AT4G35200 39.223 283 135 9 16 298 8 253 2.01e-46 157
MsG0480021277.01.T01 AT4G35690 34.783 299 163 11 13 301 8 284 3.73e-42 147
MsG0480021277.01.T01 AT4G35710 31.313 297 173 9 14 301 9 283 5.45e-29 112
MsG0480021277.01.T01 AT2G17680 29.642 307 168 11 16 300 11 291 8.38e-27 107
MsG0480021277.01.T01 AT4G35680 29.618 314 196 11 4 301 6 310 2.26e-20 89.7
MsG0480021277.01.T01 AT4G35720 28.308 325 196 11 7 300 6 324 2.31e-20 89.7
MsG0480021277.01.T01 AT1G76210 29.555 247 143 6 57 301 9 226 1.96e-19 85.5
MsG0480021277.01.T01 AT3G51400 29.412 204 130 4 100 300 82 274 8.61e-18 82.0
MsG0480021277.01.T01 AT4G35660 27.815 302 182 7 9 299 10 286 6.97e-15 73.9
MsG0480021277.01.T01 AT1G20520 26.506 249 152 6 54 300 6 225 9.20e-13 67.0

Find 49 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGAACCAATTGGAAAATT+TGG 0.112535 4:-56200823 MsG0480021277.01.T01:CDS
TGAGAAAATTCTTGGCTTCT+AGG 0.199154 4:-56201208 MsG0480021277.01.T01:CDS
ATTTATACAACTAGTGATTA+AGG 0.290293 4:+56200718 None:intergenic
AATCGAAACCAAGTAATTAT+TGG 0.313179 4:-56200989 MsG0480021277.01.T01:CDS
GGCCGAGGTGAGAAAATTCT+TGG 0.322610 4:-56201216 MsG0480021277.01.T01:CDS
ACATGATTGCGTCGAAAGTT+TGG 0.329591 4:-56201432 MsG0480021277.01.T01:CDS
TGATGTGATGTTGGACTAAT+AGG 0.335874 4:+56201596 None:intergenic
ATTATCTCCTAGCAACAATA+AGG 0.373881 4:-56201111 MsG0480021277.01.T01:CDS
TCAAATTCTCCAAAGCTTTG+AGG 0.381031 4:+56201165 None:intergenic
CAATCATTTGCAGAACCAAT+TGG 0.396017 4:-56200832 MsG0480021277.01.T01:CDS
GAAAGCAATCCTCAAAGCTT+TGG 0.399242 4:-56201174 MsG0480021277.01.T01:CDS
CAAAGCTTTGGAGAATTTGA+AGG 0.400637 4:-56201162 MsG0480021277.01.T01:CDS
ACCAAGGACCAATAATTACT+TGG 0.401947 4:+56200981 None:intergenic
TCCACGCACGTCAAGAGAAA+TGG 0.417810 4:-56201379 MsG0480021277.01.T01:CDS
ACCAAGTAATTATTGGTCCT+TGG 0.430617 4:-56200982 MsG0480021277.01.T01:CDS
TTTAATCTTTATATCTGGAC+CGG 0.469654 4:-56201015 MsG0480021277.01.T01:CDS
ATCAACTCTTCATCGTTGTT+CGG 0.473180 4:-56201482 MsG0480021277.01.T01:CDS
TTGCTACAGAACACACATCT+AGG 0.484586 4:+56201324 None:intergenic
GTCTGTTGTTCAAGGGTTTG+TGG 0.488801 4:-56200799 MsG0480021277.01.T01:CDS
AGTGATCACTCTTTCAATGT+TGG 0.496374 4:-56201045 MsG0480021277.01.T01:CDS
ACAAGGACTTGTTGAGTAAG+AGG 0.502400 4:+56201401 None:intergenic
ATTTGGAGTCTGTTGTTCAA+GGG 0.504312 4:-56200806 MsG0480021277.01.T01:CDS
CGAAATGGAGGTGACGGCCG+AGG 0.520093 4:-56201231 MsG0480021277.01.T01:CDS
AAAATTCTTGGCTTCTAGGA+AGG 0.521847 4:-56201204 MsG0480021277.01.T01:CDS
TCTCTTGACGTGCGTGGACA+AGG 0.524756 4:+56201384 None:intergenic
TCATGATCCTTATTGTTGCT+AGG 0.526232 4:+56201104 None:intergenic
TTGAAGGCTAATGTGAAGAA+AGG 0.526883 4:-56201146 MsG0480021277.01.T01:CDS
GCTAATGTGAAGAAAGGTAA+AGG 0.528831 4:-56201140 MsG0480021277.01.T01:CDS
AATTTGGAGTCTGTTGTTCA+AGG 0.538470 4:-56200807 MsG0480021277.01.T01:CDS
TCCATTTCTCTTGACGTGCG+TGG 0.539554 4:+56201378 None:intergenic
CATTGTAGAATGAGAGGGTG+TGG 0.553149 4:+56201542 None:intergenic
CGAGGTTGGTGATGTGATGT+TGG 0.563264 4:+56201587 None:intergenic
AGGAGGCGAAATGGAGGTGA+CGG 0.564930 4:-56201237 MsG0480021277.01.T01:CDS
ATGAGAGGGTGTGGTTTCGA+TGG 0.575082 4:+56201551 None:intergenic
GAAGAGTTGATTGCGTCGAA+AGG 0.582245 4:+56201494 None:intergenic
GCTTATGATGCAGAGCAAGA+AGG 0.582612 4:-56200952 MsG0480021277.01.T01:CDS
TCTACAATGCAATCAACACT+TGG 0.582703 4:-56201528 MsG0480021277.01.T01:CDS
AGATGTGTGTTCTGTAGCAA+AGG 0.585332 4:-56201321 MsG0480021277.01.T01:CDS
CATCATAAGCTTTGAAACCA+AGG 0.589484 4:+56200965 None:intergenic
AGTGATTAAGGACATTGAGA+AGG 0.590750 4:+56200730 None:intergenic
GTGATTAAGGACATTGAGAA+GGG 0.597371 4:+56200731 None:intergenic
TTACTTGGTTTCGATTGCGC+CGG 0.602841 4:+56200996 None:intergenic
GGTTAGTTTGTTAAGAGACG+TGG 0.607951 4:-56201069 MsG0480021277.01.T01:CDS
TGATTGCATTGTAGAATGAG+AGG 0.610266 4:+56201536 None:intergenic
ATCATGTAAAGCTTGTAGAT+CGG 0.622289 4:+56201448 None:intergenic
GGATTCTCTAATTCATACGA+AGG 0.644514 4:-56201300 MsG0480021277.01.T01:CDS
TGATGTTGGACTAATAGGAA+TGG 0.654192 4:+56201601 None:intergenic
AGATCCGTCTAAAAGCTCAT+CGG 0.663380 4:+56201355 None:intergenic
GATTGCATTGTAGAATGAGA+GGG 0.668472 4:+56201537 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCACTTTTAATCTTTATATC+TGG - Chr4:56201319-56201338 MsG0480021277.01.T01:CDS 20.0%
! AATCGAAACCAAGTAATTAT+TGG - Chr4:56201350-56201369 MsG0480021277.01.T01:CDS 25.0%
! TTTAATCTTTATATCTGGAC+CGG - Chr4:56201324-56201343 MsG0480021277.01.T01:CDS 25.0%
ATCATGTAAAGCTTGTAGAT+CGG + Chr4:56200894-56200913 None:intergenic 30.0%
ATTATCTCCTAGCAACAATA+AGG - Chr4:56201228-56201247 MsG0480021277.01.T01:CDS 30.0%
ACCAAGGACCAATAATTACT+TGG + Chr4:56201361-56201380 None:intergenic 35.0%
ACCAAGTAATTATTGGTCCT+TGG - Chr4:56201357-56201376 MsG0480021277.01.T01:CDS 35.0%
AGTGATCACTCTTTCAATGT+TGG - Chr4:56201294-56201313 MsG0480021277.01.T01:CDS 35.0%
AGTGATTAAGGACATTGAGA+AGG + Chr4:56201612-56201631 None:intergenic 35.0%
ATCAACTCTTCATCGTTGTT+CGG - Chr4:56200857-56200876 MsG0480021277.01.T01:CDS 35.0%
ATTATGCGAAGAAAAAGAGG+AGG - Chr4:56201085-56201104 MsG0480021277.01.T01:CDS 35.0%
CAATCATTTGCAGAACCAAT+TGG - Chr4:56201507-56201526 MsG0480021277.01.T01:CDS 35.0%
GATTGCATTGTAGAATGAGA+GGG + Chr4:56200805-56200824 None:intergenic 35.0%
GCAGAACCAATTGGAAAATT+TGG - Chr4:56201516-56201535 MsG0480021277.01.T01:CDS 35.0%
GCTAATGTGAAGAAAGGTAA+AGG - Chr4:56201199-56201218 MsG0480021277.01.T01:CDS 35.0%
GGATCATGAAAAAGTGACTT+TGG - Chr4:56201249-56201268 MsG0480021277.01.T01:CDS 35.0%
GGATTCTCTAATTCATACGA+AGG - Chr4:56201039-56201058 MsG0480021277.01.T01:CDS 35.0%
GTGATTAAGGACATTGAGAA+GGG + Chr4:56201611-56201630 None:intergenic 35.0%
TCAAATTCTCCAAAGCTTTG+AGG + Chr4:56201177-56201196 None:intergenic 35.0%
TCATGATCCTTATTGTTGCT+AGG + Chr4:56201238-56201257 None:intergenic 35.0%
TCGATTATGCGAAGAAAAAG+AGG - Chr4:56201082-56201101 MsG0480021277.01.T01:CDS 35.0%
TCTACAATGCAATCAACACT+TGG - Chr4:56200811-56200830 MsG0480021277.01.T01:CDS 35.0%
TGAGAAAATTCTTGGCTTCT+AGG - Chr4:56201131-56201150 MsG0480021277.01.T01:CDS 35.0%
TGATGTGATGTTGGACTAAT+AGG + Chr4:56200746-56200765 None:intergenic 35.0%
TGATGTTGGACTAATAGGAA+TGG + Chr4:56200741-56200760 None:intergenic 35.0%
TGATTGCATTGTAGAATGAG+AGG + Chr4:56200806-56200825 None:intergenic 35.0%
TTGAAGGCTAATGTGAAGAA+AGG - Chr4:56201193-56201212 MsG0480021277.01.T01:CDS 35.0%
! AAAATTCTTGGCTTCTAGGA+AGG - Chr4:56201135-56201154 MsG0480021277.01.T01:CDS 35.0%
! AATTTGGAGTCTGTTGTTCA+AGG - Chr4:56201532-56201551 MsG0480021277.01.T01:CDS 35.0%
! AGCTTTTAGACGGATCTTTA+AGG - Chr4:56200990-56201009 MsG0480021277.01.T01:CDS 35.0%
! ATTTGGAGTCTGTTGTTCAA+GGG - Chr4:56201533-56201552 MsG0480021277.01.T01:CDS 35.0%
! CAGACTCCAAATTTTCCAAT+TGG + Chr4:56201525-56201544 None:intergenic 35.0%
!! CAAAGCTTTGGAGAATTTGA+AGG - Chr4:56201177-56201196 MsG0480021277.01.T01:CDS 35.0%
!! CATCATAAGCTTTGAAACCA+AGG + Chr4:56201377-56201396 None:intergenic 35.0%
AAGAAAAAGAGGAGGCGAAA+TGG - Chr4:56201093-56201112 MsG0480021277.01.T01:CDS 40.0%
ACATGATTGCGTCGAAAGTT+TGG - Chr4:56200907-56200926 MsG0480021277.01.T01:CDS 40.0%
AGATCCGTCTAAAAGCTCAT+CGG + Chr4:56200987-56201006 None:intergenic 40.0%
AGATGTGTGTTCTGTAGCAA+AGG - Chr4:56201018-56201037 MsG0480021277.01.T01:CDS 40.0%
GAAAGCAATCCTCAAAGCTT+TGG - Chr4:56201165-56201184 MsG0480021277.01.T01:CDS 40.0%
TTGCTACAGAACACACATCT+AGG + Chr4:56201018-56201037 None:intergenic 40.0%
! AGCCAAGAATTTTCTCACCT+CGG + Chr4:56201128-56201147 None:intergenic 40.0%
!! ACAAGGACTTGTTGAGTAAG+AGG + Chr4:56200941-56200960 None:intergenic 40.0%
!! GGTTAGTTTGTTAAGAGACG+TGG - Chr4:56201270-56201289 MsG0480021277.01.T01:CDS 40.0%
AAAAAGAGGAGGCGAAATGG+AGG - Chr4:56201096-56201115 MsG0480021277.01.T01:CDS 45.0%
CATTGTAGAATGAGAGGGTG+TGG + Chr4:56200800-56200819 None:intergenic 45.0%
GCTTATGATGCAGAGCAAGA+AGG - Chr4:56201387-56201406 MsG0480021277.01.T01:CDS 45.0%
GTCTGTTGTTCAAGGGTTTG+TGG - Chr4:56201540-56201559 MsG0480021277.01.T01:CDS 45.0%
TTACTTGGTTTCGATTGCGC+CGG + Chr4:56201346-56201365 None:intergenic 45.0%
! GAAGAGTTGATTGCGTCGAA+AGG + Chr4:56200848-56200867 None:intergenic 45.0%
! TGGACCGATGAGCTTTTAGA+CGG - Chr4:56200980-56200999 MsG0480021277.01.T01:CDS 45.0%
GGCCGAGGTGAGAAAATTCT+TGG - Chr4:56201123-56201142 MsG0480021277.01.T01:CDS 50.0%
TCCACGCACGTCAAGAGAAA+TGG - Chr4:56200960-56200979 MsG0480021277.01.T01:CDS 50.0%
TCCATTTCTCTTGACGTGCG+TGG + Chr4:56200964-56200983 None:intergenic 50.0%
! ATGAGAGGGTGTGGTTTCGA+TGG + Chr4:56200791-56200810 None:intergenic 50.0%
!! CGAGGTTGGTGATGTGATGT+TGG + Chr4:56200755-56200774 None:intergenic 50.0%
AGGAGGCGAAATGGAGGTGA+CGG - Chr4:56201102-56201121 MsG0480021277.01.T01:CDS 55.0%
TCTCTTGACGTGCGTGGACA+AGG + Chr4:56200958-56200977 None:intergenic 55.0%
!! GATGGCAAGCTTTTCGAGCG+AGG + Chr4:56200773-56200792 None:intergenic 55.0%
!! GCAAGCTTTTCGAGCGAGGT+TGG + Chr4:56200769-56200788 None:intergenic 55.0%
! CGAAATGGAGGTGACGGCCG+AGG - Chr4:56201108-56201127 MsG0480021277.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 56200728 56201633 56200728 ID=MsG0480021277.01;Name=MsG0480021277.01
Chr4 mRNA 56200728 56201633 56200728 ID=MsG0480021277.01.T01;Parent=MsG0480021277.01;Name=MsG0480021277.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|301
Chr4 exon 56200728 56201633 56200728 ID=MsG0480021277.01.T01:exon:1251;Parent=MsG0480021277.01.T01
Chr4 CDS 56200728 56201633 56200728 ID=MsG0480021277.01.T01:cds;Parent=MsG0480021277.01.T01
Gene Sequence

>MsG0480021277.01.T01

ATGGCTACTACCATTCCTATTAGTCCAACATCACATCACCAACCTCGCTCGAAAAGCTTGCCATCGAAACCACACCCTCTCATTCTACAATGCAATCAACACTTGGCAAATTTAGAGCCTTTCGACGCAATCAACTCTTCATCGTTGTTCGGTAAAAAACTAACCGATCTACAAGCTTTACATGATTGCGTCGAAAGTTTGGTATTGCTACCTCTTACTCAACAAGTCCTTGTCCACGCACGTCAAGAGAAATGGACCGATGAGCTTTTAGACGGATCTTTAAGGCTCCTAGATGTGTGTTCTGTAGCAAAGGATTCTCTAATTCATACGAAGGAATGTGCACGCGAACTTCAATCGATTATGCGAAGAAAAAGAGGAGGCGAAATGGAGGTGACGGCCGAGGTGAGAAAATTCTTGGCTTCTAGGAAGGTAGTAAAGAAAGCAATCCTCAAAGCTTTGGAGAATTTGAAGGCTAATGTGAAGAAAGGTAAAGGAAAACTATTATCTCCTAGCAACAATAAGGATCATGAAAAAGTGACTTTGGTTAGTTTGTTAAGAGACGTGGAAGTGATCACTCTTTCAATGTTGGAGTCACTTTTAATCTTTATATCTGGACCGGCGCAATCGAAACCAAGTAATTATTGGTCCTTGGTTTCAAAGCTTATGATGCAGAGCAAGAAGGTTTCTTCTACACAAGAAGCTGATATCAACGAGTTTTCTAATGTTGATGCTGCATTGCAGTCTTTTGTGTTTAATGTCGCAAGAAAATCAGATAATGTCAATCATTTGCAGAACCAATTGGAAAATTTGGAGTCTGTTGTTCAAGGGTTTGTGGAAGCACTTGAAGCTCTTTTTAAACGCTTTATTAAGATTAGAGTTTCCCTTCTCAATGTCCTTAATCACTAG

Protein sequence

>MsG0480021277.01.T01

MATTIPISPTSHHQPRSKSLPSKPHPLILQCNQHLANLEPFDAINSSSLFGKKLTDLQALHDCVESLVLLPLTQQVLVHARQEKWTDELLDGSLRLLDVCSVAKDSLIHTKECARELQSIMRRKRGGEMEVTAEVRKFLASRKVVKKAILKALENLKANVKKGKGKLLSPSNNKDHEKVTLVSLLRDVEVITLSMLESLLIFISGPAQSKPSNYWSLVSKLMMQSKKVSSTQEADINEFSNVDAALQSFVFNVARKSDNVNHLQNQLENLESVVQGFVEALEALFKRFIKIRVSLLNVLNH*