AlfalfaGEDB | MsG0280011450.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011450.01

KEGG: -
GO: GO:0046983
PFAM: PF00010

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011450.01.T01	AES68218.2	77.5	280	46	5	1	273	1	270	1.74E-145	419

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011450.01.T01	Q9T072	39.157	166	96	4	103	267	152	313	2.77E-26	108

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049040.01	MsG0280011450.01	0.819855	9.327452e-53	7.261871e-50
MsG0080049068.01	MsG0280011450.01	0.805625	1.219502e-49	6.482950e-47
MsG0180001270.01	MsG0280011450.01	0.813178	2.919277e-51	1.892834e-48
MsG0280011450.01	MsG0380017703.01	0.800787	1.223107e-48	5.745589e-46
MsG0280011450.01	MsG0480019284.01	0.800357	1.496727e-48	6.955140e-46
MsG0280011450.01	MsG0480021814.01	0.800241	1.580797e-48	7.323757e-46
MsG0280011450.01	MsG0580024873.01	0.805771	1.136711e-49	6.065722e-47
MsG0280011450.01	MsG0580029312.01	0.808590	2.874694e-50	1.650150e-47
MsG0280011450.01	MsG0680031469.01	0.812380	4.365997e-51	2.771651e-48
MsG0280011450.01	MsG0680034064.01	0.800869	1.177238e-48	5.541946e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011450.01.T01	MTR_2g104500	77.500	280	46	5	1	273	1	270	2.11e-149	419
MsG0280011450.01.T01	MTR_2g104530	63.273	275	62	9	1	258	1	253	5.00e-102	299
MsG0280011450.01.T01	MTR_2g104490	42.705	281	132	9	2	260	16	289	4.78e-52	172
MsG0280011450.01.T01	MTR_2g104490	42.294	279	132	9	2	258	16	287	3.55e-51	170
MsG0280011450.01.T01	MTR_2g104550	42.199	282	134	11	1	258	15	291	8.71e-51	169
MsG0280011450.01.T01	MTR_4g009540	42.396	217	114	4	50	258	97	310	3.97e-44	152
MsG0280011450.01.T01	MTR_4g009540	41.935	217	115	4	50	258	97	310	2.99e-43	150
MsG0280011450.01.T01	MTR_4g009110	40.909	220	117	3	50	258	112	329	6.89e-43	150
MsG0280011450.01.T01	MTR_8g009020	48.148	162	76	4	103	258	143	302	2.38e-36	132
MsG0280011450.01.T01	MTR_2g104560	44.048	168	82	5	104	268	55	213	1.30e-34	125

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011450.01.T01	AT4G37850	39.157	166	96	4	103	267	152	313	2.82e-27	108
MsG0280011450.01.T01	AT4G37850	39.157	166	96	4	103	267	152	313	2.82e-27	108
MsG0280011450.01.T01	AT2G22760	37.037	162	100	2	103	263	119	279	1.63e-26	105
MsG0280011450.01.T01	AT2G22760	36.747	166	102	3	103	267	119	282	4.21e-26	104
MsG0280011450.01.T01	AT2G22770	35.955	178	101	3	103	268	132	308	2.24e-23	97.8
MsG0280011450.01.T01	AT2G22750	35.196	179	90	4	103	268	126	291	1.33e-18	84.3
MsG0280011450.01.T01	AT2G22750	35.196	179	90	4	103	268	154	319	1.66e-18	84.3
MsG0280011450.01.T01	AT2G22750	35.196	179	90	4	103	268	150	315	2.04e-18	84.0
MsG0280011450.01.T01	AT2G22750	35.714	168	83	3	103	257	126	281	2.93e-18	83.2
MsG0280011450.01.T01	AT2G22750	35.714	168	83	3	103	257	154	309	3.21e-18	83.6

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Find 48 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGGCAATGTGCTAGTGC+TGG	0.603776	2:-84173531	None:intergenic
AAAGGAAGTCTTCATCTTCG+TGG	0.550705	2:-84172234	None:intergenic
AAATTTGGTGATGCGAACCT+TGG	0.588692	2:-84173585	None:intergenic
AAGATTCATTTCACTAGATG+TGG	0.558723	2:-84173360	None:intergenic
AATGCAAGCTCTAGAGGATG+TGG	0.535694	2:-84172353	None:intergenic
ACAACGTGGTGGTTTGGATT+TGG	0.302005	2:-84172422	None:intergenic
ACATCAGGAAGCAATTCATT+TGG	0.411022	2:-84173398	None:intergenic
ACGCAATGGGAGAGAGGAAA+AGG	0.480162	2:+84172515	MsG0280011450.01.T01:CDS
ACGCTCGGTAGCAGCAGCAA+TGG	0.570572	2:-84172290	None:intergenic
AGACTTCCTTTGTGACTTCA+TGG	0.350365	2:+84172246	MsG0280011450.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACATCTCTTCAAATCGT+TGG	+	Chr2:84172780-84172799	MsG0280011450.01.T01:intron	30.0%
AAGATTCATTTCACTAGATG+TGG	-	Chr2:84173363-84173382	None:intergenic	30.0%
AGTAGAATCTACCTTTAAAC+CGG	+	Chr2:84173137-84173156	MsG0280011450.01.T01:intron	30.0%
ATCAATTGTGAGAAGGAAAA+TGG	+	Chr2:84173458-84173477	MsG0280011450.01.T01:CDS	30.0%
CATTTACATCAATTGTGAGA+AGG	+	Chr2:84173451-84173470	MsG0280011450.01.T01:CDS	30.0%
GTATCAACAACAAATGTTGA+GGG	-	Chr2:84172871-84172890	None:intergenic	30.0%
TGACATAATACCATTCTTCA+GGG	+	Chr2:84172820-84172839	MsG0280011450.01.T01:intron	30.0%
TGGCATTCAACACAAAATAT+TGG	+	Chr2:84173478-84173497	MsG0280011450.01.T01:CDS	30.0%
TTGACATAATACCATTCTTC+AGG	+	Chr2:84172819-84172838	MsG0280011450.01.T01:intron	30.0%
ACATCAGGAAGCAATTCATT+TGG	-	Chr2:84173401-84173420	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	84172205	84173664	84172205	ID=MsG0280011450.01;Name=MsG0280011450.01
Chr2	mRNA	84172205	84173664	84172205	ID=MsG0280011450.01.T01;Parent=MsG0280011450.01;Name=MsG0280011450.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|1\|0\|1\|1\|1\|2\|0\|281
Chr2	exon	84172205	84172597	84172205	ID=MsG0280011450.01.T01:exon:12609;Parent=MsG0280011450.01.T01
Chr2	exon	84173212	84173664	84173212	ID=MsG0280011450.01.T01:exon:12610;Parent=MsG0280011450.01.T01
Chr2	CDS	84172205	84172597	84172205	ID=MsG0280011450.01.T01:cds;Parent=MsG0280011450.01.T01
Chr2	CDS	84173212	84173664	84173212	ID=MsG0280011450.01.T01:cds;Parent=MsG0280011450.01.T01

Gene Sequence

>MsG0280011450.01.T01

ATGGATGTGGAATATTTGTTAGAGATGTGCCACGAAGATGAAGACTTCCTTTGTGACTTCATGGCACAGCCAGTAGCTGGAGCTGCCATTGCTGCTGCTACCGAGCGTATTGTAGTCTCTGATGAGTCACCGGAGCTTCTTCCTCCGTCCACATCCTCTAGAGCTTGCATTATCTCTTTGGATAATTCCGCGAAGATACTGTTACCAGCTGTGATGTCCAAATCCAAACCACCACGTTGTCCCACAAAAAAGATGATGACTTCTGAGAATCGAAACATGGAACCAAAAGCAATTATTAGATCTCTAGATCACGCAATGGGAGAGAGGAAAAGGAGACTTGAACTTGCACACAAATTCATAGAGCTTTCAGCTATTATTCCTCGCTCAAACAAGACAGACAAAGCTTCTATCGTTGCTGGCGCAACCAATTACGTGGAGCAACTCCAGAAACGAGTGAAGGAGTTAGAAGTACAACAAAACAAGAGAGGTAAAGAACCAATGATACTTCTCAATAAGAAAAATTCTTGTGGAGTTTCACGTACCACATCTAGTGAAATGAATCTTGACAATTATCTTGGACCAAATGAATTGCTTCCTGATGTCAAAGTGAAAGTGTCGGAGAATAATATACTCATTTACATCAATTGTGAGAAGGAAAATGGCATTCAACACAAAATATTGGACATGCTTCAAAATCTTCATTTGTTTGTCACCAGCACTAGCACATTGCCGTTTGGCAATTCAACTCTTGGCATAACTATCATCGCCAAGGTTCGCATCACCAAATTTTCATTAACTATTGAAATGAATATGTTTTTAAGGGAAATGTTGTTGAATGAATGTTGA

Protein sequence

>MsG0280011450.01.T01

MDVEYLLEMCHEDEDFLCDFMAQPVAGAAIAAATERIVVSDESPELLPPSTSSRACIISLDNSAKILLPAVMSKSKPPRCPTKKMMTSENRNMEPKAIIRSLDHAMGERKRRLELAHKFIELSAIIPRSNKTDKASIVAGATNYVEQLQKRVKELEVQQNKRGKEPMILLNKKNSCGVSRTTSSEMNLDNYLGPNELLPDVKVKVSENNILIYINCEKENGIQHKILDMLQNLHLFVTSTSTLPFGNSTLGITIIAKVRITKFSLTIEMNMFLREMLLNEC*