AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024873.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024873.01.T01 MTR_5g018590 95.031 161 8 0 1 161 1 161 7.53e-105 297
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024873.01.T01 AT2G23670 64.706 119 42 0 43 161 49 167 4.28e-44 143

Find 58 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGTGGTGAATGGTATTT+TGG 0.123106 5:-11089613 MsG0580024873.01.T01:CDS
GGGAGTGGGGCTTGGGAGTT+TGG 0.256873 5:-11089688 MsG0580024873.01.T01:CDS
GGCAAGGGAATCGGTGTCTA+TGG 0.348419 5:-11089883 MsG0580024873.01.T01:CDS
TAGAGGCGGGAGTGGGGCTT+GGG 0.373454 5:-11089695 MsG0580024873.01.T01:CDS
ATAGAGGCGGGAGTGGGGCT+TGG 0.382300 5:-11089696 MsG0580024873.01.T01:CDS
GACGGCGTCTACTGCTTTAA+CGG 0.384186 5:-11089904 MsG0580024873.01.T01:CDS
CGCAGGATATTCCTCTGTTC+GGG 0.384628 5:-11089860 MsG0580024873.01.T01:CDS
GCGCAGGATATTCCTCTGTT+CGG 0.388532 5:-11089861 MsG0580024873.01.T01:CDS
TGCTTTAACGGCAAGGGAAT+CGG 0.400881 5:-11089892 MsG0580024873.01.T01:CDS
AGGATTGGAGGAGGCGGAGA+AGG 0.404820 5:-11089721 MsG0580024873.01.T01:CDS
GTTGCTTCGGCGGTGGTGAA+TGG 0.416909 5:-11089621 MsG0580024873.01.T01:CDS
GAAGGAGATAGAGGCGGGAG+TGG 0.419322 5:-11089703 MsG0580024873.01.T01:CDS
GTAACAGCGTCTCCATCATC+CGG 0.439205 5:-11089951 MsG0580024873.01.T01:CDS
AGACGCTGTTACGGAGTTTG+TGG 0.442900 5:+11089962 None:intergenic
GAAGGCGGAAGTAGGATTGG+AGG 0.443492 5:-11089733 MsG0580024873.01.T01:CDS
CACGTTGAAAGGAGAGAGTT+GGG 0.450299 5:+11090027 None:intergenic
GGGGCTTGGGAGTTTGGCAC+AGG 0.455310 5:-11089682 MsG0580024873.01.T01:CDS
AAGGAGATAGAGGCGGGAGT+GGG 0.465218 5:-11089702 MsG0580024873.01.T01:CDS
AGTCATGACTTAGAACCTTC+AGG 0.466701 5:+11089588 None:intergenic
GGATTTGAATCGGCGGAGAA+AGG 0.475547 5:-11089783 MsG0580024873.01.T01:CDS
AGCTCCCTTCGCCCGGATGA+TGG 0.478842 5:+11089939 None:intergenic
ATATTCCTCTGTTCGGGATA+CGG 0.481304 5:-11089854 MsG0580024873.01.T01:CDS
AGGAGATAGAGGCGGGAGTG+GGG 0.482697 5:-11089701 MsG0580024873.01.T01:CDS
GGAGGCGGAGAAGGAGATAG+AGG 0.485080 5:-11089712 MsG0580024873.01.T01:CDS
TGAGAAGGCGGAAGTAGGAT+TGG 0.487305 5:-11089736 MsG0580024873.01.T01:CDS
GGATTGGAGACGGCGGAGAA+AGG 0.490920 5:-11089762 MsG0580024873.01.T01:CDS
GCACAGGCGATTGTGGTGGC+CGG 0.505389 5:-11089666 MsG0580024873.01.T01:CDS
GCTGAGGAGATTGTGAAGGA+AGG 0.507532 5:-11089804 MsG0580024873.01.T01:CDS
CGTCTCCATCATCCGGGCGA+AGG 0.507772 5:-11089944 MsG0580024873.01.T01:CDS
ACACGTTGAAAGGAGAGAGT+TGG 0.515766 5:+11090026 None:intergenic
TGAATCGGCGGAGAAAGGAT+TGG 0.515965 5:-11089778 MsG0580024873.01.T01:CDS
AAACAGCAGCTCCCTTCGCC+CGG 0.527420 5:+11089932 None:intergenic
GGACTTGAGAAGGCGGAAGT+AGG 0.529383 5:-11089741 MsG0580024873.01.T01:CDS
GTCTCCATCATCCGGGCGAA+GGG 0.529471 5:-11089943 MsG0580024873.01.T01:CDS
TGTGAAGGAAGGATTTGAAT+CGG 0.532246 5:-11089793 MsG0580024873.01.T01:CDS
GGAAGTAGGATTGGAGGAGG+CGG 0.536091 5:-11089727 MsG0580024873.01.T01:CDS
GGATGATGGAGACGCTGTTA+CGG 0.541392 5:+11089953 None:intergenic
GAGTTTGGCACAGGCGATTG+TGG 0.547955 5:-11089673 MsG0580024873.01.T01:CDS
GATTGTGGTGGCCGGAGCAG+AGG 0.555003 5:-11089658 MsG0580024873.01.T01:CDS
GCGAGCGATGACACGTTGAA+AGG 0.558528 5:+11090016 None:intergenic
AAAAGCTGAGGAGATTGTGA+AGG 0.558586 5:-11089808 MsG0580024873.01.T01:CDS
GCGGAGAAGGAGATAGAGGC+GGG 0.560902 5:-11089708 MsG0580024873.01.T01:CDS
GTCTACTGCTTTAACGGCAA+GGG 0.563427 5:-11089898 MsG0580024873.01.T01:CDS
GGAGCTGCTGTTTCTTCTGA+CGG 0.565357 5:-11089922 MsG0580024873.01.T01:CDS
TTTGGCACAGGCGATTGTGG+TGG 0.576692 5:-11089670 MsG0580024873.01.T01:CDS
GGCGGAGAAAGGACTTGAGA+AGG 0.576857 5:-11089751 MsG0580024873.01.T01:CDS
GGAATCGGTGTCTATGGCGC+AGG 0.576983 5:-11089877 MsG0580024873.01.T01:CDS
GGCGGAAGTAGGATTGGAGG+AGG 0.581019 5:-11089730 MsG0580024873.01.T01:CDS
CGTCTACTGCTTTAACGGCA+AGG 0.585643 5:-11089899 MsG0580024873.01.T01:CDS
TAACAGCGTCTCCATCATCC+GGG 0.601839 5:-11089950 MsG0580024873.01.T01:CDS
GTGTGTCGTGAAGCAATGGT+TGG 0.601927 5:-11090068 None:intergenic
GGCGGAGAAGGAGATAGAGG+CGG 0.609342 5:-11089709 MsG0580024873.01.T01:CDS
GGCGGAGAAAGGATTGGAGA+CGG 0.612290 5:-11089772 MsG0580024873.01.T01:CDS
TGTGTCGTGAAGCAATGGTT+GGG 0.636465 5:-11090067 None:intergenic
GGAGAAAGGATTGGAGACGG+CGG 0.673797 5:-11089769 MsG0580024873.01.T01:CDS
GGAGAAAGGACTTGAGAAGG+CGG 0.676092 5:-11089748 MsG0580024873.01.T01:CDS
CTCTTCCGTATCCCGAACAG+AGG 0.679487 5:+11089849 None:intergenic
GAAGGAAGGATTTGAATCGG+CGG 0.697190 5:-11089790 MsG0580024873.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGTGAAGGAAGGATTTGAAT+CGG - Chr5:11089850-11089869 MsG0580024873.01.T01:CDS 35.0%
!! TTTTGGTCTTTTGGTTGCTT+CGG - Chr5:11090009-11090028 MsG0580024873.01.T01:CDS 35.0%
AAAAGCTGAGGAGATTGTGA+AGG - Chr5:11089835-11089854 MsG0580024873.01.T01:CDS 40.0%
ATATTCCTCTGTTCGGGATA+CGG - Chr5:11089789-11089808 MsG0580024873.01.T01:CDS 40.0%
GAAAGCAGAAGAAAAAGCTG+AGG - Chr5:11089823-11089842 MsG0580024873.01.T01:CDS 40.0%
!!! AGCAGAGGTTTTTGGTCTTT+TGG - Chr5:11090000-11090019 MsG0580024873.01.T01:CDS 40.0%
AAGACCAAAAACCTCTGCTC+CGG + Chr5:11089999-11090018 None:intergenic 45.0%
ACACGTTGAAAGGAGAGAGT+TGG + Chr5:11089620-11089639 None:intergenic 45.0%
CACGTTGAAAGGAGAGAGTT+GGG + Chr5:11089619-11089638 None:intergenic 45.0%
GAAGGAAGGATTTGAATCGG+CGG - Chr5:11089853-11089872 MsG0580024873.01.T01:CDS 45.0%
GTCTACTGCTTTAACGGCAA+GGG - Chr5:11089745-11089764 MsG0580024873.01.T01:CDS 45.0%
TGCTTTAACGGCAAGGGAAT+CGG - Chr5:11089751-11089770 MsG0580024873.01.T01:CDS 45.0%
! CGGTGGTGAATGGTATTTTG+GGG - Chr5:11090032-11090051 MsG0580024873.01.T01:CDS 45.0%
! GCGGTGGTGAATGGTATTTT+GGG - Chr5:11090031-11090050 MsG0580024873.01.T01:CDS 45.0%
!!! AATGGTATTTTGGGGCCTGA+AGG - Chr5:11090040-11090059 MsG0580024873.01.T01:CDS 45.0%
AGACGCTGTTACGGAGTTTG+TGG + Chr5:11089684-11089703 None:intergenic 50.0%
CGCAGGATATTCCTCTGTTC+GGG - Chr5:11089783-11089802 MsG0580024873.01.T01:CDS 50.0%
CGTCTACTGCTTTAACGGCA+AGG - Chr5:11089744-11089763 MsG0580024873.01.T01:CDS 50.0%
GACGGCGTCTACTGCTTTAA+CGG - Chr5:11089739-11089758 MsG0580024873.01.T01:CDS 50.0%
GCGCAGGATATTCCTCTGTT+CGG - Chr5:11089782-11089801 MsG0580024873.01.T01:CDS 50.0%
GCTGAGGAGATTGTGAAGGA+AGG - Chr5:11089839-11089858 MsG0580024873.01.T01:CDS 50.0%
GGAGAAAGGACTTGAGAAGG+CGG - Chr5:11089895-11089914 MsG0580024873.01.T01:CDS 50.0%
GGATTTGAATCGGCGGAGAA+AGG - Chr5:11089860-11089879 MsG0580024873.01.T01:CDS 50.0%
GTAACAGCGTCTCCATCATC+CGG - Chr5:11089692-11089711 MsG0580024873.01.T01:CDS 50.0%
TAACAGCGTCTCCATCATCC+GGG - Chr5:11089693-11089712 MsG0580024873.01.T01:CDS 50.0%
TGAATCGGCGGAGAAAGGAT+TGG - Chr5:11089865-11089884 MsG0580024873.01.T01:CDS 50.0%
TGAGAAGGCGGAAGTAGGAT+TGG - Chr5:11089907-11089926 MsG0580024873.01.T01:CDS 50.0%
! GGCGGTGGTGAATGGTATTT+TGG - Chr5:11090030-11090049 MsG0580024873.01.T01:CDS 50.0%
!! GGAGCTGCTGTTTCTTCTGA+CGG - Chr5:11089721-11089740 MsG0580024873.01.T01:CDS 50.0%
!! GGATGATGGAGACGCTGTTA+CGG + Chr5:11089693-11089712 None:intergenic 50.0%
!! TGGTCTTTTGGTTGCTTCGG+CGG - Chr5:11090012-11090031 MsG0580024873.01.T01:CDS 50.0%
AAGGAGATAGAGGCGGGAGT+GGG - Chr5:11089941-11089960 MsG0580024873.01.T01:CDS 55.0%
CTCTTCCGTATCCCGAACAG+AGG + Chr5:11089797-11089816 None:intergenic 55.0%
GAAGGCGGAAGTAGGATTGG+AGG - Chr5:11089910-11089929 MsG0580024873.01.T01:CDS 55.0%
GAGTTTGGCACAGGCGATTG+TGG - Chr5:11089970-11089989 MsG0580024873.01.T01:CDS 55.0%
GCGAGCGATGACACGTTGAA+AGG + Chr5:11089630-11089649 None:intergenic 55.0%
GGAAGTAGGATTGGAGGAGG+CGG - Chr5:11089916-11089935 MsG0580024873.01.T01:CDS 55.0%
GGACTTGAGAAGGCGGAAGT+AGG - Chr5:11089902-11089921 MsG0580024873.01.T01:CDS 55.0%
GGAGAAAGGATTGGAGACGG+CGG - Chr5:11089874-11089893 MsG0580024873.01.T01:CDS 55.0%
GGCGGAGAAAGGACTTGAGA+AGG - Chr5:11089892-11089911 MsG0580024873.01.T01:CDS 55.0%
GGCGGAGAAAGGATTGGAGA+CGG - Chr5:11089871-11089890 MsG0580024873.01.T01:CDS 55.0%
TTTGGCACAGGCGATTGTGG+TGG - Chr5:11089973-11089992 MsG0580024873.01.T01:CDS 55.0%
! GGCAAGGGAATCGGTGTCTA+TGG - Chr5:11089760-11089779 MsG0580024873.01.T01:CDS 55.0%
!! TCTTTTGGTTGCTTCGGCGG+TGG - Chr5:11090015-11090034 MsG0580024873.01.T01:CDS 55.0%
AAACAGCAGCTCCCTTCGCC+CGG + Chr5:11089714-11089733 None:intergenic 60.0%
AGGAGATAGAGGCGGGAGTG+GGG - Chr5:11089942-11089961 MsG0580024873.01.T01:CDS 60.0%
AGGATTGGAGGAGGCGGAGA+AGG - Chr5:11089922-11089941 MsG0580024873.01.T01:CDS 60.0%
GAAGGAGATAGAGGCGGGAG+TGG - Chr5:11089940-11089959 MsG0580024873.01.T01:CDS 60.0%
GCGGAGAAGGAGATAGAGGC+GGG - Chr5:11089935-11089954 MsG0580024873.01.T01:CDS 60.0%
GGAGGCGGAGAAGGAGATAG+AGG - Chr5:11089931-11089950 MsG0580024873.01.T01:CDS 60.0%
GGATTGGAGACGGCGGAGAA+AGG - Chr5:11089881-11089900 MsG0580024873.01.T01:CDS 60.0%
GGCGGAAGTAGGATTGGAGG+AGG - Chr5:11089913-11089932 MsG0580024873.01.T01:CDS 60.0%
GGCGGAGAAGGAGATAGAGG+CGG - Chr5:11089934-11089953 MsG0580024873.01.T01:CDS 60.0%
GTCTCCATCATCCGGGCGAA+GGG - Chr5:11089700-11089719 MsG0580024873.01.T01:CDS 60.0%
GTTGCTTCGGCGGTGGTGAA+TGG - Chr5:11090022-11090041 MsG0580024873.01.T01:CDS 60.0%
! GGAATCGGTGTCTATGGCGC+AGG - Chr5:11089766-11089785 MsG0580024873.01.T01:CDS 60.0%
! GTGGCCGGAGCAGAGGTTTT+TGG - Chr5:11089992-11090011 MsG0580024873.01.T01:CDS 60.0%
AGCTCCCTTCGCCCGGATGA+TGG + Chr5:11089707-11089726 None:intergenic 65.0%
ATAGAGGCGGGAGTGGGGCT+TGG - Chr5:11089947-11089966 MsG0580024873.01.T01:CDS 65.0%
CGTCTCCATCATCCGGGCGA+AGG - Chr5:11089699-11089718 MsG0580024873.01.T01:CDS 65.0%
GATTGTGGTGGCCGGAGCAG+AGG - Chr5:11089985-11090004 MsG0580024873.01.T01:CDS 65.0%
GCACAGGCGATTGTGGTGGC+CGG - Chr5:11089977-11089996 MsG0580024873.01.T01:CDS 65.0%
GGGAGTGGGGCTTGGGAGTT+TGG - Chr5:11089955-11089974 MsG0580024873.01.T01:CDS 65.0%
GGGGCTTGGGAGTTTGGCAC+AGG - Chr5:11089961-11089980 MsG0580024873.01.T01:CDS 65.0%
TAGAGGCGGGAGTGGGGCTT+GGG - Chr5:11089948-11089967 MsG0580024873.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 11089590 11090075 11089590 ID=MsG0580024873.01;Name=MsG0580024873.01
Chr5 mRNA 11089590 11090075 11089590 ID=MsG0580024873.01.T01;Parent=MsG0580024873.01;Name=MsG0580024873.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|161
Chr5 exon 11089590 11090075 11089590 ID=MsG0580024873.01.T01:exon:3093;Parent=MsG0580024873.01.T01
Chr5 CDS 11089590 11090075 11089590 ID=MsG0580024873.01.T01:cds;Parent=MsG0580024873.01.T01
Gene Sequence

>MsG0580024873.01.T01

ATGGTTGGGTATGCAATCTCGTCGTTCCCAACTCTCTCCTTTCAACGTGTCATCGCTCGCAATGCGACGTCGTTTTCATGCAAAGCGAAAGCCACAAACTCCGTAACAGCGTCTCCATCATCCGGGCGAAGGGAGCTGCTGTTTCTTCTGACGGCGTCTACTGCTTTAACGGCAAGGGAATCGGTGTCTATGGCGCAGGATATTCCTCTGTTCGGGATACGGAAGAGTTTGAAGAAAGCAGAAGAAAAAGCTGAGGAGATTGTGAAGGAAGGATTTGAATCGGCGGAGAAAGGATTGGAGACGGCGGAGAAAGGACTTGAGAAGGCGGAAGTAGGATTGGAGGAGGCGGAGAAGGAGATAGAGGCGGGAGTGGGGCTTGGGAGTTTGGCACAGGCGATTGTGGTGGCCGGAGCAGAGGTTTTTGGTCTTTTGGTTGCTTCGGCGGTGGTGAATGGTATTTTGGGGCCTGAAGGTTCTAAGTCATGA

Protein sequence

>MsG0580024873.01.T01

MVGYAISSFPTLSFQRVIARNATSFSCKAKATNSVTASPSSGRRELLFLLTASTALTARESVSMAQDIPLFGIRKSLKKAEEKAEEIVKEGFESAEKGLETAEKGLEKAEVGLEEAEKEIEAGVGLGSLAQAIVVAGAEVFGLLVASAVVNGILGPEGSKS*