AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380011531.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011531.01.T01 MTR_3g006190 77.011 87 15 3 1 85 1 84 1.27e-37 124
MsG0380011531.01.T01 MTR_4g030060 45.122 82 40 3 6 85 1 79 1.01e-13 62.4
MsG0380011531.01.T01 MTR_4g030060 43.902 82 41 3 6 85 1 79 1.37e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011531.01.T01 AT1G33490 56.322 87 33 3 1 85 1 84 2.85e-22 84.0
MsG0380011531.01.T01 AT1G33490 56.322 87 33 3 1 85 1 84 6.94e-22 84.0
MsG0380011531.01.T01 AT4G10140 50.000 86 40 2 1 85 1 84 7.53e-20 79.0
MsG0380011531.01.T01 AT1G33490 80.000 35 7 0 51 85 8 42 2.76e-13 60.8

Find 16 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATGCTCACGAACTTTA+TGG 0.165738 3:-1008673 MsG0380011531.01.T01:CDS
GCATATTACCTCAACTCTAT+TGG 0.377682 3:+1008645 None:intergenic
CTCTTGTTTCCATTCTTCAC+AGG 0.437303 3:-1010465 MsG0380011531.01.T01:CDS
TTACAATAACAAACAAATAG+TGG 0.443287 3:+1010407 None:intergenic
GGAAGATGGCTATTACCAAT+TGG 0.470067 3:-1010510 MsG0380011531.01.T01:CDS
TGAAGAGTACCTGTGAAGAA+TGG 0.505109 3:+1010456 None:intergenic
GGAATGGCTATTGCATTGCT+TGG 0.517080 3:-1008717 MsG0380011531.01.T01:CDS
CACTGTATTCCTCTGCGGAA+TGG 0.522128 3:-1008733 MsG0380011531.01.T01:CDS
TTTGAACGAAGATGGCGAAT+GGG 0.528659 3:-1010531 None:intergenic
GAAGATGGCGAATGGGAAGA+TGG 0.532129 3:-1010524 None:intergenic
CTTTGAACGAAGATGGCGAA+TGG 0.546126 3:-1010532 None:intergenic
AAGTTCGTGAGCATCATAGT+TGG 0.557950 3:+1008678 None:intergenic
TGGCGACGCCAATAGAGTTG+AGG 0.614691 3:-1008653 MsG0380011531.01.T01:CDS
CTGTGAAGAATGGAAACAAG+AGG 0.624294 3:+1010466 None:intergenic
ATGCAATAGCCATTCCGCAG+AGG 0.697698 3:+1008724 None:intergenic
GGTGTCACTGTATTCCTCTG+CGG 0.703627 3:-1008738 MsG0380011531.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTCTTTATATGAT+TGG - Chr3:1009822-1009841 MsG0380011531.01.T01:intron 10.0%
!! AAAGAAAAAAAAAAACAAAC+TGG + Chr3:1009815-1009834 None:intergenic 15.0%
!!! AATTTTATTTCTGTAATGAT+TGG - Chr3:1008805-1008824 MsG0380011531.01.T01:intron 15.0%
!! ATATATATCCAGGAAATTAT+GGG + Chr3:1009422-1009441 None:intergenic 20.0%
!! ATTGAATTACAGTTATCTTT+AGG + Chr3:1009585-1009604 None:intergenic 20.0%
!! TTACAATAACAAACAAATAG+TGG + Chr3:1008766-1008785 None:intergenic 20.0%
!! TTAGATATGAAATGTCTTTA+TGG - Chr3:1009521-1009540 MsG0380011531.01.T01:intron 20.0%
!!! ATAATCAAAGTCAGTTTTAA+AGG + Chr3:1008892-1008911 None:intergenic 20.0%
!!! TGATTTTGATATGTTTTGTT+CGG - Chr3:1009105-1009124 MsG0380011531.01.T01:intron 20.0%
!!! TTAAAGAAACTAATTTTGAG+TGG + Chr3:1009323-1009342 None:intergenic 20.0%
!!! TTATTTTAGCATATATATCC+AGG + Chr3:1009432-1009451 None:intergenic 20.0%
!!! TTCTTTGAAAAAAGTGATTT+TGG - Chr3:1008966-1008985 MsG0380011531.01.T01:intron 20.0%
! AAGCTAAAGTGATTTATGTT+TGG + Chr3:1009261-1009280 None:intergenic 25.0%
! AATTACAACATACCACATAT+AGG + Chr3:1009690-1009709 None:intergenic 25.0%
! CATATATATCCAGGAAATTA+TGG + Chr3:1009423-1009442 None:intergenic 25.0%
! CTCATGAAATTAAGCTTAAA+AGG + Chr3:1009952-1009971 None:intergenic 25.0%
! TATATGGATGTGAAATATGT+CGG - Chr3:1010365-1010384 MsG0380011531.01.T01:intron 25.0%
! TCTGCAATATGTTAAATATG+AGG - Chr3:1010392-1010411 MsG0380011531.01.T01:intron 25.0%
! TCTTTATATGATTGGACATT+GGG - Chr3:1009830-1009849 MsG0380011531.01.T01:intron 25.0%
! TGATATCAAGCATAAATAAG+AGG + Chr3:1009472-1009491 None:intergenic 25.0%
! TGGTTAATGATTATGCTAAA+TGG + Chr3:1009795-1009814 None:intergenic 25.0%
! TTCTTTATATGATTGGACAT+TGG - Chr3:1009829-1009848 MsG0380011531.01.T01:intron 25.0%
!! GATTTTTGCACTCAAATTAA+TGG - Chr3:1009202-1009221 MsG0380011531.01.T01:intron 25.0%
!! TAAAACTGACTTTGATTATG+AGG - Chr3:1008893-1008912 MsG0380011531.01.T01:intron 25.0%
!! TGTTTGTAAAAGTGTATGTT+TGG - Chr3:1009038-1009057 MsG0380011531.01.T01:intron 25.0%
!! TTTTTGTTCATGCTATAAGT+GGG + Chr3:1009997-1010016 None:intergenic 25.0%
!!! AAGTGATTTTGGTATCAAAA+AGG - Chr3:1008977-1008996 MsG0380011531.01.T01:intron 25.0%
!!! AATTTTGGTTAATCCAAAAG+TGG + Chr3:1009066-1009085 None:intergenic 25.0%
!!! AGTGATTTTGGTATCAAAAA+GGG - Chr3:1008978-1008997 MsG0380011531.01.T01:intron 25.0%
!!! ATTTTGCTATGTTTTTTCAG+AGG - Chr3:1009606-1009625 MsG0380011531.01.T01:intron 25.0%
!!! ATTTTGGTTAATCCAAAAGT+GGG + Chr3:1009065-1009084 None:intergenic 25.0%
AAAAGAGTTGTAACTGTGTA+CGG - Chr3:1010245-1010264 MsG0380011531.01.T01:intron 30.0%
AACTATACCGTCATTTATGT+AGG - Chr3:1009728-1009747 MsG0380011531.01.T01:intron 30.0%
ACTTTGATTATGAGGTATAG+GGG - Chr3:1008901-1008920 MsG0380011531.01.T01:intron 30.0%
AGTGATTTATGTTTGGACTT+TGG + Chr3:1009254-1009273 None:intergenic 30.0%
ATAAATGACGGTATAGTTCA+AGG + Chr3:1009726-1009745 None:intergenic 30.0%
GAATCATACAATGTCATAAC+TGG + Chr3:1010119-1010138 None:intergenic 30.0%
GACTTTGATTATGAGGTATA+GGG - Chr3:1008900-1008919 MsG0380011531.01.T01:intron 30.0%
GGTTATATGTGATCTGTTTA+TGG - Chr3:1008826-1008845 MsG0380011531.01.T01:intron 30.0%
TAAGTGAAAGAAAGTAAGTG+CGG - Chr3:1010316-1010335 MsG0380011531.01.T01:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG + Chr3:1009856-1009875 None:intergenic 30.0%
TGACTTTGATTATGAGGTAT+AGG - Chr3:1008899-1008918 MsG0380011531.01.T01:intron 30.0%
TGCAAATACTAAACAAGACA+TGG + Chr3:1009771-1009790 None:intergenic 30.0%
! AAATTTTTCTTAGCTGTGGT+TGG + Chr3:1010148-1010167 None:intergenic 30.0%
! AAGTTGTGACGTTTTTCTTT+TGG - Chr3:1009549-1009568 MsG0380011531.01.T01:intron 30.0%
! ACAGAAATTTTTCTTAGCTG+TGG + Chr3:1010152-1010171 None:intergenic 30.0%
! GGATTAACCAAAATTGATTC+TGG - Chr3:1009071-1009090 MsG0380011531.01.T01:intron 30.0%
! GTTTTTGTTCATGCTATAAG+TGG + Chr3:1009998-1010017 None:intergenic 30.0%
! TTAACCAAAATTGATTCTGG+AGG - Chr3:1009074-1009093 MsG0380011531.01.T01:intron 30.0%
!! TCACTTTTAGAAGTTCTAAC+AGG + Chr3:1009347-1009366 None:intergenic 30.0%
ACTAAACAAGACATGGTTGT+GGG + Chr3:1009764-1009783 None:intergenic 35.0%
CACATCCATATATGCTTTCT+CGG + Chr3:1010357-1010376 None:intergenic 35.0%
CTGCATTCCTACATAAATGA+CGG + Chr3:1009738-1009757 None:intergenic 35.0%
TACTAAACAAGACATGGTTG+TGG + Chr3:1009765-1009784 None:intergenic 35.0%
TATGATGCTCACGAACTTTA+TGG - Chr3:1010497-1010516 MsG0380011531.01.T01:CDS 35.0%
TTAAGCTGTTTGTCCAAACA+GGG - Chr3:1010093-1010112 MsG0380011531.01.T01:intron 35.0%
! GAGGCTAATGTTTTGCAAAT+AGG - Chr3:1010411-1010430 MsG0380011531.01.T01:CDS 35.0%
! TGGATTCTATCACTTGTTGA+AGG - Chr3:1008846-1008865 MsG0380011531.01.T01:intron 35.0%
!! ATAGTTCAAGGTGGGTTAAT+TGG + Chr3:1009714-1009733 None:intergenic 35.0%
!! TTGGGAGTATTTCCTATATG+TGG - Chr3:1009675-1009694 MsG0380011531.01.T01:intron 35.0%
!!! GATTTTCTTCTGGAACCAAT+TGG + Chr3:1008678-1008697 None:intergenic 35.0%
AAGTTCGTGAGCATCATAGT+TGG + Chr3:1010495-1010514 None:intergenic 40.0%
AATGACGGTATAGTTCAAGG+TGG + Chr3:1009723-1009742 None:intergenic 40.0%
ATGACGGTATAGTTCAAGGT+GGG + Chr3:1009722-1009741 None:intergenic 40.0%
CAATTGTGCCCATAATTTCC+TGG - Chr3:1009411-1009430 MsG0380011531.01.T01:intron 40.0%
CTAAACAAGACATGGTTGTG+GGG + Chr3:1009763-1009782 None:intergenic 40.0%
CTGTGAAGAATGGAAACAAG+AGG + Chr3:1008707-1008726 None:intergenic 40.0%
GGAAGATGGCTATTACCAAT+TGG - Chr3:1008660-1008679 MsG0380011531.01.T01:CDS 40.0%
GTGGGTTTCATAAGCTATCT+TGG + Chr3:1009979-1009998 None:intergenic 40.0%
GTTAAGCTGTTTGTCCAAAC+AGG - Chr3:1010092-1010111 MsG0380011531.01.T01:intron 40.0%
TTGTTCGGCAAGAGAAGAAT+TGG - Chr3:1009120-1009139 MsG0380011531.01.T01:intron 40.0%
! AGGATCCGAGAAAGCATATA+TGG - Chr3:1010349-1010368 MsG0380011531.01.T01:intron 40.0%
! CTCTTGTTTCCATTCTTCAC+AGG - Chr3:1008705-1008724 MsG0380011531.01.T01:CDS 40.0%
! TGCACCTCCAGAATCAATTT+TGG + Chr3:1009081-1009100 None:intergenic 40.0%
! TGTATGTTTGGTCCCACTTT+TGG - Chr3:1009050-1009069 MsG0380011531.01.T01:intron 40.0%
!! TGAAGAGTACCTGTGAAGAA+TGG + Chr3:1008717-1008736 None:intergenic 40.0%
!!! GAGGATCACTGATTTTCTTC+TGG + Chr3:1008688-1008707 None:intergenic 40.0%
AGGAGCTGAACCAAATCAGT+TGG - Chr3:1009626-1009645 MsG0380011531.01.T01:intron 45.0%
ATGTCATAACTGGCCCTGTT+TGG + Chr3:1010109-1010128 None:intergenic 45.0%
GCAGAGAAAGCCAACTGATT+TGG + Chr3:1009639-1009658 None:intergenic 45.0%
GCTGCTCTTGGCATTACATT+GGG - Chr3:1009657-1009676 MsG0380011531.01.T01:intron 45.0%
GGAATGGCTATTGCATTGCT+TGG - Chr3:1010453-1010472 MsG0380011531.01.T01:CDS 45.0%
TGCTGCTCTTGGCATTACAT+TGG - Chr3:1009656-1009675 MsG0380011531.01.T01:intron 45.0%
ATGCAATAGCCATTCCGCAG+AGG + Chr3:1010449-1010468 None:intergenic 50.0%
CACTGTATTCCTCTGCGGAA+TGG - Chr3:1010437-1010456 MsG0380011531.01.T01:CDS 50.0%
GATTGGACATTGGGCCTGTT+TGG - Chr3:1009839-1009858 MsG0380011531.01.T01:intron 50.0%
TTTCTCGGATCCTCTACAGC+AGG + Chr3:1010342-1010361 None:intergenic 50.0%
! TTGGCTTTCTCTGCTGCTCT+TGG - Chr3:1009645-1009664 MsG0380011531.01.T01:intron 50.0%
!! GGTGTCACTGTATTCCTCTG+CGG - Chr3:1010432-1010451 MsG0380011531.01.T01:CDS 50.0%
GATCCTCTACAGCAGCTGCA+TGG - Chr3:1010267-1010286 MsG0380011531.01.T01:intron 55.0%
TGGCGACGCCAATAGAGTTG+AGG - Chr3:1010517-1010536 MsG0380011531.01.T01:CDS 55.0%
GCACCATGCAGCTGCTGTAG+AGG + Chr3:1010273-1010292 None:intergenic 60.0%
GCAGCACACTGCACAGCTGT+TGG - Chr3:1010291-1010310 MsG0380011531.01.T01:intron 60.0%
GTAAGTGCGGCCTGCTGTAG+AGG - Chr3:1010329-1010348 MsG0380011531.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 1008650 1010542 1008650 ID=MsG0380011531.01;Name=MsG0380011531.01
Chr3 mRNA 1008650 1010542 1008650 ID=MsG0380011531.01.T01;Parent=MsG0380011531.01;Name=MsG0380011531.01.T01;_AED=0.49;_eAED=0.57;_QI=0|0|0|1|0|0|2|0|86
Chr3 exon 1010411 1010542 1010411 ID=MsG0380011531.01.T01:exon:677;Parent=MsG0380011531.01.T01
Chr3 exon 1008650 1008778 1008650 ID=MsG0380011531.01.T01:exon:676;Parent=MsG0380011531.01.T01
Chr3 CDS 1010411 1010542 1010411 ID=MsG0380011531.01.T01:cds;Parent=MsG0380011531.01.T01
Chr3 CDS 1008650 1008778 1008650 ID=MsG0380011531.01.T01:cds;Parent=MsG0380011531.01.T01
Gene Sequence

>MsG0380011531.01.T01

ATGGCGAATGGGAAGATGGCTATTACCAATTGGTTCCAGAAGAAAATCAGTGATCCTCTTGTTTCCATTCTTCACAGGTACTCTTCAATTTCTTCACTTTTTTCAGCTGTGATCCACTATTTGTTTGTTATTGCTAATGTTTTGCAAATAGGTGTCACTGTATTCCTCTGCGGAATGGCTATTGCATTGCTTGGATCATATTGTCATGCTCCAACTATGATGCTCACGAACTTTATGGCGACGCCAATAGAGTTGAGGTAA

Protein sequence

>MsG0380011531.01.T01

MANGKMAITNWFQKKISDPLVSILHRYSSISSLFSAVIHYLFVIANVLQIGVTVFLCGMAIALLGSYCHAPTMMLTNFMATPIELR*