AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000787.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000787.01.T01 MTR_1g008450 93.939 198 12 0 1 198 116 313 1.55e-139 392
MsG0180000787.01.T01 MTR_1g008470 79.293 198 41 0 1 198 103 300 1.89e-116 333
MsG0180000787.01.T01 MTR_1g008500 77.612 201 42 3 1 198 185 385 2.18e-107 313
MsG0180000787.01.T01 MTR_1g008530 74.000 200 50 2 1 198 25 224 6.99e-107 306
MsG0180000787.01.T01 MTR_0312s0030 74.874 199 49 1 1 198 127 325 7.68e-107 310
MsG0180000787.01.T01 MTR_1g007520 74.874 199 49 1 1 198 127 325 7.68e-107 310
MsG0180000787.01.T01 MTR_1g008590 74.372 199 50 1 1 198 127 325 9.87e-107 309
MsG0180000787.01.T01 MTR_1g008460 74.747 198 47 1 1 198 103 297 1.18e-105 305
MsG0180000787.01.T01 MTR_1g008560 74.257 202 48 3 1 198 129 330 1.84e-105 306
MsG0180000787.01.T01 MTR_1g008510 74.500 200 47 3 1 198 129 326 5.21e-105 305
MsG0180000787.01.T01 MTR_1g008580 74.854 171 41 2 29 197 1 171 1.23e-91 265
MsG0180000787.01.T01 MTR_3g109490 54.000 200 86 4 1 195 121 319 2.55e-74 227
MsG0180000787.01.T01 MTR_3g107830 41.753 194 108 2 10 198 63 256 3.21e-51 166
MsG0180000787.01.T01 MTR_4g069680 41.753 194 108 3 10 198 94 287 3.25e-51 167
MsG0180000787.01.T01 MTR_3g116410 39.896 193 111 2 11 198 84 276 2.35e-49 162
MsG0180000787.01.T01 MTR_3g116380 40.816 196 110 3 5 195 106 300 5.08e-48 159
MsG0180000787.01.T01 MTR_3g116270 39.362 188 109 2 13 195 316 503 2.59e-42 149
MsG0180000787.01.T01 MTR_3g116320 40.426 188 107 2 13 195 498 685 4.75e-42 150
MsG0180000787.01.T01 MTR_8g064500 38.725 204 112 6 1 195 171 370 3.67e-39 138
MsG0180000787.01.T01 MTR_4g069040 35.263 190 116 4 13 195 318 507 5.43e-38 137
MsG0180000787.01.T01 MTR_4g069570 34.737 190 117 4 13 195 144 333 3.41e-37 132
MsG0180000787.01.T01 MTR_8g064560 37.696 191 108 5 12 195 437 623 6.33e-36 132
MsG0180000787.01.T01 MTR_4g069550 34.737 190 117 4 13 195 271 460 2.04e-35 129
MsG0180000787.01.T01 MTR_4g069520 34.737 190 117 4 13 195 531 720 2.65e-34 128
MsG0180000787.01.T01 MTR_4g069520 34.737 190 117 4 13 195 517 706 2.94e-34 128
MsG0180000787.01.T01 MTR_5g034320 34.737 190 115 5 12 195 440 626 6.75e-34 127
MsG0180000787.01.T01 MTR_8g445170 41.497 147 81 4 49 195 240 381 1.16e-32 121
MsG0180000787.01.T01 MTR_8g064530 35.938 192 114 5 12 197 454 642 2.56e-32 122
MsG0180000787.01.T01 MTR_6g086530 32.143 196 125 5 11 198 11 206 3.24e-32 115
MsG0180000787.01.T01 MTR_8g045890 45.522 134 68 4 62 195 301 429 5.72e-32 120
MsG0180000787.01.T01 MTR_3g078090 36.316 190 112 5 12 195 438 624 7.48e-32 121
MsG0180000787.01.T01 MTR_8g045890 45.522 134 68 4 62 195 342 470 1.02e-31 120
MsG0180000787.01.T01 MTR_8g045890 45.522 134 68 4 62 195 379 507 1.47e-31 119
MsG0180000787.01.T01 MTR_8g445140 42.537 134 72 4 62 195 172 300 2.62e-30 113
MsG0180000787.01.T01 MTR_8g046000 42.222 135 73 4 62 196 92 221 1.48e-29 109
MsG0180000787.01.T01 MTR_8g045880 44.030 134 70 4 62 195 184 312 1.64e-29 111
MsG0180000787.01.T01 MTR_8g045880 44.030 134 70 4 62 195 219 347 3.21e-29 111
MsG0180000787.01.T01 MTR_8g045880 44.030 134 70 4 62 195 221 349 3.59e-29 111
MsG0180000787.01.T01 MTR_4g133840 32.461 191 104 5 12 195 14 186 2.73e-27 102
MsG0180000787.01.T01 MTR_2g081610 36.709 158 87 6 39 195 112 257 4.76e-27 103
MsG0180000787.01.T01 MTR_1g045860 34.831 178 104 7 25 195 85 257 1.61e-25 100
MsG0180000787.01.T01 MTR_2g081770 34.375 160 86 7 48 195 116 268 1.64e-25 100
MsG0180000787.01.T01 MTR_8g044290 45.370 108 55 3 55 162 229 332 4.44e-24 98.6
MsG0180000787.01.T01 MTR_2g081590 35.556 135 82 4 61 195 128 257 1.91e-23 94.4
MsG0180000787.01.T01 MTR_5g033350 29.000 200 135 4 1 195 1 198 1.97e-23 92.8
MsG0180000787.01.T01 MTR_5g033340 29.756 205 129 6 1 195 178 377 2.66e-23 95.9
MsG0180000787.01.T01 MTR_5g033340 28.571 161 98 6 31 183 2 153 2.73e-15 73.6
MsG0180000787.01.T01 MTR_1g008420 80.769 52 10 0 1 52 148 199 1.51e-20 85.5
MsG0180000787.01.T01 MTR_5g033330 28.780 205 131 6 1 195 417 616 3.12e-20 88.2
MsG0180000787.01.T01 MTR_5g033330 29.016 193 118 7 1 183 209 392 7.90e-19 84.0
MsG0180000787.01.T01 MTR_5g033330 29.016 193 118 7 1 183 1 184 1.42e-18 83.2
MsG0180000787.01.T01 MTR_4g068420 33.083 133 71 5 66 195 1 118 1.29e-18 78.2
MsG0180000787.01.T01 MTR_5g033360 27.670 206 114 8 1 195 39 220 3.76e-16 73.9
MsG0180000787.01.T01 MTR_8g075930 39.726 73 40 2 117 186 16 87 1.21e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000787.01.T01 AT5G25610 56.614 189 79 3 12 197 203 391 4.38e-71 221
MsG0180000787.01.T01 AT1G49320 36.869 198 115 4 11 198 83 280 6.13e-43 145
MsG0180000787.01.T01 AT1G60390 36.275 204 117 5 1 195 421 620 2.64e-34 128
MsG0180000787.01.T01 AT1G70370 35.450 189 115 3 12 195 436 622 2.72e-32 122
MsG0180000787.01.T01 AT1G70370 35.450 189 115 3 12 195 436 622 2.72e-32 122
MsG0180000787.01.T01 AT1G23760 34.392 189 117 4 12 195 432 618 1.76e-30 117

Find 39 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACTTTGTTTGATGAAAA+TGG 0.206491 1:+11171384 None:intergenic
ACTATTTCAACAGAAGTAAA+TGG 0.306242 1:-11171189 MsG0180000787.01.T01:CDS
AGCAAACAGAGTGAAGAAAA+TGG 0.310868 1:-11171139 MsG0180000787.01.T01:CDS
TTCCGTCGATCCTTGCTTGA+TGG 0.311634 1:+11171341 None:intergenic
CAAGTGCTCAAAGTTCAAAA+AGG 0.316009 1:-11170934 MsG0180000787.01.T01:CDS
TGTGACATCATTGGAATCTA+TGG 0.323363 1:-11171250 MsG0180000787.01.T01:CDS
ACTTGAAAAGCCAAATGTTT+AGG 0.337232 1:+11170952 None:intergenic
GAAAATGGGAGAGAACAATT+TGG 0.374965 1:-11171124 MsG0180000787.01.T01:CDS
TACAACAGGGTCAAGTTGTT+TGG 0.384712 1:-11170888 MsG0180000787.01.T01:CDS
ACAATGGAGTCCTAAACATT+TGG 0.392896 1:-11170962 MsG0180000787.01.T01:CDS
TGACAGTAAAACACTGCATA+AGG 0.401155 1:+11171078 None:intergenic
AAAGCTTTGTGTGACATCAT+TGG 0.404983 1:-11171259 MsG0180000787.01.T01:CDS
AAACAACTTGACCCTGTTGT+AGG 0.414196 1:+11170890 None:intergenic
AGTTTGCCACATCCTACAAC+AGG 0.427433 1:-11170902 MsG0180000787.01.T01:CDS
TTGCCTCTGGAAGGTGCTGA+TGG 0.457330 1:-11171027 MsG0180000787.01.T01:CDS
GATTTCACTACTTCGAAACT+TGG 0.458312 1:-11171225 MsG0180000787.01.T01:CDS
TGTTGTAGGATGTGGCAAAC+TGG 0.462952 1:+11170904 None:intergenic
CATAAGGGTAATTCCGTTTG+TGG 0.469316 1:+11171094 None:intergenic
GCAAACAGAGTGAAGAAAAT+GGG 0.469711 1:-11171138 MsG0180000787.01.T01:CDS
ATTTGGTTGTGTGCCACAAA+CGG 0.476684 1:-11171107 MsG0180000787.01.T01:CDS
TAAAGTGTACTCTTTGCCTC+TGG 0.484430 1:-11171040 MsG0180000787.01.T01:CDS
GCTTGATGGAGAAGTAGTTA+AGG 0.487051 1:+11171355 None:intergenic
TTTGTTTGATGAAAATGGTA+TGG 0.490197 1:+11171389 None:intergenic
ATAAGTGAGTGTGAAGCATA+TGG 0.510314 1:-11171297 MsG0180000787.01.T01:CDS
GACAGTAAAACACTGCATAA+GGG 0.527238 1:+11171079 None:intergenic
ACAGAAGTAAATGGAGAAAG+TGG 0.530769 1:-11171180 MsG0180000787.01.T01:CDS
TGTGAAGCATATGGTATCAA+AGG 0.543560 1:-11171288 MsG0180000787.01.T01:CDS
CTCCATCAAGCAAGGATCGA+CGG 0.567679 1:-11171343 MsG0180000787.01.T01:CDS
GTGTACTCTTTGCCTCTGGA+AGG 0.594455 1:-11171036 MsG0180000787.01.T01:CDS
AACTACTTCTCCATCAAGCA+AGG 0.596765 1:-11171351 MsG0180000787.01.T01:CDS
CTTGACCCTGTTGTAGGATG+TGG 0.602810 1:+11170896 None:intergenic
GAGGCAAGAATGTTGGTCCA+TGG 0.603479 1:+11171427 None:intergenic
ATGGAATTAGCAACGTCGCG+AGG 0.606572 1:+11171408 None:intergenic
ACGTCGCGAGGCAAGAATGT+TGG 0.624134 1:+11171420 None:intergenic
AGCATATGGTATCAAAGGAG+AGG 0.627493 1:-11171283 MsG0180000787.01.T01:CDS
CTTCCATCAGCACCTTCCAG+AGG 0.659408 1:+11171024 None:intergenic
TTCACAAAGACATCAGACCA+TGG 0.689219 1:-11171444 MsG0180000787.01.T01:CDS
GTCACACTGATACTTCACAA+TGG 0.703281 1:-11170978 MsG0180000787.01.T01:CDS
GTTTGCCACATCCTACAACA+GGG 0.745484 1:-11170901 MsG0180000787.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACTATTTCAACAGAAGTAAA+TGG - Chr1:11171137-11171156 MsG0180000787.01.T01:CDS 25.0%
! TTTGTTTGATGAAAATGGTA+TGG + Chr1:11170940-11170959 None:intergenic 25.0%
ACTTGAAAAGCCAAATGTTT+AGG + Chr1:11171377-11171396 None:intergenic 30.0%
! ACCATTTTCATCAAACAAAG+TGG - Chr1:11170941-11170960 MsG0180000787.01.T01:CDS 30.0%
! TCCACTTTGTTTGATGAAAA+TGG + Chr1:11170945-11170964 None:intergenic 30.0%
ACAATGGAGTCCTAAACATT+TGG - Chr1:11171364-11171383 MsG0180000787.01.T01:CDS 35.0%
ACAGAAGTAAATGGAGAAAG+TGG - Chr1:11171146-11171165 MsG0180000787.01.T01:CDS 35.0%
AGCAAACAGAGTGAAGAAAA+TGG - Chr1:11171187-11171206 MsG0180000787.01.T01:CDS 35.0%
ATAAGTGAGTGTGAAGCATA+TGG - Chr1:11171029-11171048 MsG0180000787.01.T01:CDS 35.0%
CAAGTGCTCAAAGTTCAAAA+AGG - Chr1:11171392-11171411 MsG0180000787.01.T01:CDS 35.0%
GAAAATGGGAGAGAACAATT+TGG - Chr1:11171202-11171221 MsG0180000787.01.T01:CDS 35.0%
GACAGTAAAACACTGCATAA+GGG + Chr1:11171250-11171269 None:intergenic 35.0%
GATTTCACTACTTCGAAACT+TGG - Chr1:11171101-11171120 MsG0180000787.01.T01:CDS 35.0%
GCAAACAGAGTGAAGAAAAT+GGG - Chr1:11171188-11171207 MsG0180000787.01.T01:CDS 35.0%
TGACAGTAAAACACTGCATA+AGG + Chr1:11171251-11171270 None:intergenic 35.0%
TGTGAAGCATATGGTATCAA+AGG - Chr1:11171038-11171057 MsG0180000787.01.T01:CDS 35.0%
TGTGACATCATTGGAATCTA+TGG - Chr1:11171076-11171095 MsG0180000787.01.T01:CDS 35.0%
! AAAGCTTTGTGTGACATCAT+TGG - Chr1:11171067-11171086 MsG0180000787.01.T01:CDS 35.0%
AAACAACTTGACCCTGTTGT+AGG + Chr1:11171439-11171458 None:intergenic 40.0%
AACTACTTCTCCATCAAGCA+AGG - Chr1:11170975-11170994 MsG0180000787.01.T01:CDS 40.0%
AGCATATGGTATCAAAGGAG+AGG - Chr1:11171043-11171062 MsG0180000787.01.T01:CDS 40.0%
ATTTGGTTGTGTGCCACAAA+CGG - Chr1:11171219-11171238 MsG0180000787.01.T01:CDS 40.0%
CATAAGGGTAATTCCGTTTG+TGG + Chr1:11171235-11171254 None:intergenic 40.0%
GCTTGATGGAGAAGTAGTTA+AGG + Chr1:11170974-11170993 None:intergenic 40.0%
GTCACACTGATACTTCACAA+TGG - Chr1:11171348-11171367 MsG0180000787.01.T01:CDS 40.0%
TAAAGTGTACTCTTTGCCTC+TGG - Chr1:11171286-11171305 MsG0180000787.01.T01:CDS 40.0%
TTCACAAAGACATCAGACCA+TGG - Chr1:11170882-11170901 MsG0180000787.01.T01:CDS 40.0%
! TACAACAGGGTCAAGTTGTT+TGG - Chr1:11171438-11171457 MsG0180000787.01.T01:CDS 40.0%
AGTTTGCCACATCCTACAAC+AGG - Chr1:11171424-11171443 MsG0180000787.01.T01:CDS 45.0%
GTTTGCCACATCCTACAACA+GGG - Chr1:11171425-11171444 MsG0180000787.01.T01:CDS 45.0%
TGTTGTAGGATGTGGCAAAC+TGG + Chr1:11171425-11171444 None:intergenic 45.0%
ATGGAATTAGCAACGTCGCG+AGG + Chr1:11170921-11170940 None:intergenic 50.0%
CTCCATCAAGCAAGGATCGA+CGG - Chr1:11170983-11171002 MsG0180000787.01.T01:CDS 50.0%
CTTGACCCTGTTGTAGGATG+TGG + Chr1:11171433-11171452 None:intergenic 50.0%
GAGGCAAGAATGTTGGTCCA+TGG + Chr1:11170902-11170921 None:intergenic 50.0%
GTGTACTCTTTGCCTCTGGA+AGG - Chr1:11171290-11171309 MsG0180000787.01.T01:CDS 50.0%
TTCCGTCGATCCTTGCTTGA+TGG + Chr1:11170988-11171007 None:intergenic 50.0%
ACGTCGCGAGGCAAGAATGT+TGG + Chr1:11170909-11170928 None:intergenic 55.0%
CTTCCATCAGCACCTTCCAG+AGG + Chr1:11171305-11171324 None:intergenic 55.0%
!! TTGCCTCTGGAAGGTGCTGA+TGG - Chr1:11171299-11171318 MsG0180000787.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 11170876 11171472 11170876 ID=MsG0180000787.01;Name=MsG0180000787.01
Chr1 mRNA 11170876 11171472 11170876 ID=MsG0180000787.01.T01;Parent=MsG0180000787.01;Name=MsG0180000787.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|198
Chr1 exon 11170876 11171472 11170876 ID=MsG0180000787.01.T01:exon:919;Parent=MsG0180000787.01.T01
Chr1 CDS 11170876 11171472 11170876 ID=MsG0180000787.01.T01:cds;Parent=MsG0180000787.01.T01
Gene Sequence

>MsG0180000787.01.T01

ATGCAATTCACAAAGACATCAGACCATGGACCAACATTCTTGCCTCGCGACGTTGCTAATTCCATACCATTTTCATCAAACAAAGTGGAAAACATCCTTAACTACTTCTCCATCAAGCAAGGATCGACGGAATCTGAAATTGTGAAGAACACAATAAGTGAGTGTGAAGCATATGGTATCAAAGGAGAGGAAAAGCTTTGTGTGACATCATTGGAATCTATGGTTGATTTCACTACTTCGAAACTTGGAAACAATGTTGACACTATTTCAACAGAAGTAAATGGAGAAAGTGGTTTACAACAATATGTAATAGCAAACAGAGTGAAGAAAATGGGAGAGAACAATTTGGTTGTGTGCCACAAACGGAATTACCCTTATGCAGTGTTTTACTGTCACAAAACAGATGCAACTAAAGTGTACTCTTTGCCTCTGGAAGGTGCTGATGGAAGTAGAGTTAAAGCTGTTGCTATTTGTCACACTGATACTTCACAATGGAGTCCTAAACATTTGGCTTTTCAAGTGCTCAAAGTTCAAAAAGGAACTGTTCCAGTTTGCCACATCCTACAACAGGGTCAAGTTGTTTGGTTTTCCAAGTAA

Protein sequence

>MsG0180000787.01.T01

MQFTKTSDHGPTFLPRDVANSIPFSSNKVENILNYFSIKQGSTESEIVKNTISECEAYGIKGEEKLCVTSLESMVDFTTSKLGNNVDTISTEVNGESGLQQYVIANRVKKMGENNLVVCHKRNYPYAVFYCHKTDATKVYSLPLEGADGSRVKAVAICHTDTSQWSPKHLAFQVLKVQKGTVPVCHILQQGQVVWFSK*