Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011722.01.T01 | GAU16364.1 | 79.121 | 91 | 15 | 2 | 28 | 118 | 49 | 135 | 1.71E-30 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011722.01.T01 | Q8RXR2 | 43.86 | 114 | 45 | 5 | 23 | 118 | 41 | 153 | 7.99E-15 | 72.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011722.01.T01 | A0A1S2Y5E1 | 82.022 | 89 | 13 | 1 | 30 | 118 | 50 | 135 | 5.67e-28 | 117 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000741.01 | MsG0380011722.01 | 0.805521 | 1.282142e-49 | 6.797534e-47 |
| MsG0380011721.01 | MsG0380011722.01 | 0.811677 | 6.212908e-51 | 3.869801e-48 |
| MsG0380011722.01 | MsG0380016307.01 | 0.817002 | 4.133512e-52 | 2.974669e-49 |
| MsG0380011722.01 | MsG0480021216.01 | 0.801679 | 8.036558e-49 | 3.861218e-46 |
| MsG0380011722.01 | MsG0480021900.01 | 0.817913 | 2.576724e-52 | 1.901077e-49 |
| MsG0380011722.01 | MsG0480022552.01 | 0.820015 | 8.571894e-53 | 6.703928e-50 |
| MsG0380011722.01 | MsG0480022598.01 | 0.801217 | 9.994841e-49 | 4.746617e-46 |
| MsG0380011722.01 | MsG0680034757.01 | 0.801310 | 9.565331e-49 | 4.553372e-46 |
| MsG0280007048.01 | MsG0380011722.01 | 0.803143 | 4.012572e-49 | 2.001139e-46 |
| MsG0280007726.01 | MsG0380011722.01 | 0.800443 | 1.438110e-48 | 6.697014e-46 |
| MsG0280007819.01 | MsG0380011722.01 | 0.807243 | 5.558816e-50 | 3.080617e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011722.01.T01 | MTR_3g009530 | 91.011 | 89 | 6 | 1 | 30 | 118 | 45 | 131 | 2.01e-27 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011722.01.T01 | AT1G55620 | 43.860 | 114 | 45 | 5 | 23 | 118 | 41 | 153 | 8.15e-16 | 72.8 |
Find 31 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATTCATTTGATTCTTC+TGG | 0.175525 | 3:-4073991 | MsG0380011722.01.T01:CDS |
| CTTGTTGCTGCTTTCAATAA+AGG | 0.292178 | 3:-4073889 | MsG0380011722.01.T01:CDS |
| TTGCTCCTTGGTTGTCTCCT+TGG | 0.310407 | 3:-4073928 | MsG0380011722.01.T01:CDS |
| GAACACATCGCCGGAGTTAA+TGG | 0.314664 | 3:-4074184 | MsG0380011722.01.T01:CDS |
| TTGTTGCTGCTTTCAATAAA+GGG | 0.333792 | 3:-4073888 | MsG0380011722.01.T01:CDS |
| GTGGTGGTGGCGGTAGGGAG+TGG | 0.367731 | 3:-4074159 | MsG0380011722.01.T01:CDS |
| AGAGATATGTTGAAATTATC+AGG | 0.385849 | 3:-4074126 | MsG0380011722.01.T01:CDS |
| GTGGCGGTAGGGAGTGGTAA+AGG | 0.386769 | 3:-4074153 | MsG0380011722.01.T01:CDS |
| AGGAGCAACAAAGCCCATTC+AGG | 0.387645 | 3:+4073943 | None:intergenic |
| ATGGCGGTGGTGGTGGCGGT+AGG | 0.396836 | 3:-4074165 | MsG0380011722.01.T01:CDS |
| GAATGGGCTTTGTTGCTCCT+TGG | 0.397932 | 3:-4073940 | MsG0380011722.01.T01:CDS |
| TGTCTCCTTGGTCTTGCCAC+TGG | 0.413211 | 3:-4073916 | MsG0380011722.01.T01:CDS |
| CTGATAGTGCACCTCCTGAA+TGG | 0.436429 | 3:-4073957 | MsG0380011722.01.T01:CDS |
| GGAGTTAATGGCGGTGGTGG+TGG | 0.440137 | 3:-4074172 | MsG0380011722.01.T01:CDS |
| GTTAATGGCGGTGGTGGTGG+CGG | 0.462911 | 3:-4074169 | MsG0380011722.01.T01:CDS |
| TGGCGGTGGTGGTGGCGGTA+GGG | 0.477404 | 3:-4074164 | MsG0380011722.01.T01:CDS |
| TGATAGTGCACCTCCTGAAT+GGG | 0.483817 | 3:-4073956 | MsG0380011722.01.T01:CDS |
| GCCGGAGTTAATGGCGGTGG+TGG | 0.496160 | 3:-4074175 | MsG0380011722.01.T01:CDS |
| TGGCGGTAGGGAGTGGTAAA+GGG | 0.501445 | 3:-4074152 | MsG0380011722.01.T01:CDS |
| ACCACCACCGCCATTAACTC+CGG | 0.524738 | 3:+4074174 | None:intergenic |
| TGTTGCTGCTTTCAATAAAG+GGG | 0.543037 | 3:-4073887 | MsG0380011722.01.T01:CDS |
| CACGGATCGGAACACATCGC+CGG | 0.548171 | 3:-4074193 | MsG0380011722.01.T01:CDS |
| ATCGCCGGAGTTAATGGCGG+TGG | 0.561251 | 3:-4074178 | MsG0380011722.01.T01:CDS |
| GGAGACCAGTGGCAAGACCA+AGG | 0.612140 | 3:+4073911 | None:intergenic |
| GAACGAGACATTGAAGCTCA+CGG | 0.614125 | 3:-4074211 | MsG0380011722.01.T01:CDS |
| CTTTATTGAAAGCAGCAACA+AGG | 0.617678 | 3:+4073890 | None:intergenic |
| AGACATTGAAGCTCACGGAT+CGG | 0.631337 | 3:-4074206 | MsG0380011722.01.T01:CDS |
| AGCAACAAAGCCCATTCAGG+AGG | 0.640278 | 3:+4073946 | None:intergenic |
| AGCAGCAACAAGGAGACCAG+TGG | 0.683119 | 3:+4073900 | None:intergenic |
| CAAGACCAAGGAGACAACCA+AGG | 0.688884 | 3:+4073923 | None:intergenic |
| CACATCGCCGGAGTTAATGG+CGG | 0.705098 | 3:-4074181 | MsG0380011722.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAGATATGTTGAAATTATC+AGG | - | Chr3:4073957-4073976 | MsG0380011722.01.T01:CDS | 25.0% |
| CTTCATTCATTTGATTCTTC+TGG | - | Chr3:4074092-4074111 | MsG0380011722.01.T01:CDS | 30.0% | |
| !! | TTGTTGCTGCTTTCAATAAA+GGG | - | Chr3:4074195-4074214 | MsG0380011722.01.T01:CDS | 30.0% |
| CTTTATTGAAAGCAGCAACA+AGG | + | Chr3:4074196-4074215 | None:intergenic | 35.0% | |
| !! | CTTGTTGCTGCTTTCAATAA+AGG | - | Chr3:4074194-4074213 | MsG0380011722.01.T01:CDS | 35.0% |
| !! | TGTTGCTGCTTTCAATAAAG+GGG | - | Chr3:4074196-4074215 | MsG0380011722.01.T01:CDS | 35.0% |
| AGACATTGAAGCTCACGGAT+CGG | - | Chr3:4073877-4073896 | MsG0380011722.01.T01:CDS | 45.0% | |
| GAACGAGACATTGAAGCTCA+CGG | - | Chr3:4073872-4073891 | MsG0380011722.01.T01:CDS | 45.0% | |
| ! | TGATAGTGCACCTCCTGAAT+GGG | - | Chr3:4074127-4074146 | MsG0380011722.01.T01:CDS | 45.0% |
| AGCAACAAAGCCCATTCAGG+AGG | + | Chr3:4074140-4074159 | None:intergenic | 50.0% | |
| AGGAGCAACAAAGCCCATTC+AGG | + | Chr3:4074143-4074162 | None:intergenic | 50.0% | |
| CAAGACCAAGGAGACAACCA+AGG | + | Chr3:4074163-4074182 | None:intergenic | 50.0% | |
| GAACACATCGCCGGAGTTAA+TGG | - | Chr3:4073899-4073918 | MsG0380011722.01.T01:CDS | 50.0% | |
| TTGCTCCTTGGTTGTCTCCT+TGG | - | Chr3:4074155-4074174 | MsG0380011722.01.T01:CDS | 50.0% | |
| ! | CTGATAGTGCACCTCCTGAA+TGG | - | Chr3:4074126-4074145 | MsG0380011722.01.T01:CDS | 50.0% |
| !! | GAATGGGCTTTGTTGCTCCT+TGG | - | Chr3:4074143-4074162 | MsG0380011722.01.T01:CDS | 50.0% |
| ACCACCACCGCCATTAACTC+CGG | + | Chr3:4073912-4073931 | None:intergenic | 55.0% | |
| AGCAGCAACAAGGAGACCAG+TGG | + | Chr3:4074186-4074205 | None:intergenic | 55.0% | |
| CACATCGCCGGAGTTAATGG+CGG | - | Chr3:4073902-4073921 | MsG0380011722.01.T01:CDS | 55.0% | |
| TGGCGGTAGGGAGTGGTAAA+GGG | - | Chr3:4073931-4073950 | MsG0380011722.01.T01:CDS | 55.0% | |
| !! | TGTCTCCTTGGTCTTGCCAC+TGG | - | Chr3:4074167-4074186 | MsG0380011722.01.T01:CDS | 55.0% |
| ATCGCCGGAGTTAATGGCGG+TGG | - | Chr3:4073905-4073924 | MsG0380011722.01.T01:CDS | 60.0% | |
| CACGGATCGGAACACATCGC+CGG | - | Chr3:4073890-4073909 | MsG0380011722.01.T01:CDS | 60.0% | |
| GGAGACCAGTGGCAAGACCA+AGG | + | Chr3:4074175-4074194 | None:intergenic | 60.0% | |
| GTGGCGGTAGGGAGTGGTAA+AGG | - | Chr3:4073930-4073949 | MsG0380011722.01.T01:CDS | 60.0% | |
| ! | GGAGTTAATGGCGGTGGTGG+TGG | - | Chr3:4073911-4073930 | MsG0380011722.01.T01:CDS | 60.0% |
| !! | GTTAATGGCGGTGGTGGTGG+CGG | - | Chr3:4073914-4073933 | MsG0380011722.01.T01:CDS | 60.0% |
| GCCGGAGTTAATGGCGGTGG+TGG | - | Chr3:4073908-4073927 | MsG0380011722.01.T01:CDS | 65.0% | |
| !! | ATGGCGGTGGTGGTGGCGGT+AGG | - | Chr3:4073918-4073937 | MsG0380011722.01.T01:CDS | 70.0% |
| !! | GTGGTGGTGGCGGTAGGGAG+TGG | - | Chr3:4073924-4073943 | MsG0380011722.01.T01:CDS | 70.0% |
| !! | TGGCGGTGGTGGTGGCGGTA+GGG | - | Chr3:4073919-4073938 | MsG0380011722.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 4073867 | 4074238 | 4073867 | ID=MsG0380011722.01;Name=MsG0380011722.01 |
| Chr3 | mRNA | 4073867 | 4074238 | 4073867 | ID=MsG0380011722.01.T01;Parent=MsG0380011722.01;Name=MsG0380011722.01.T01;_AED=0.50;_eAED=0.59;_QI=0|-1|0|1|-1|1|1|0|123 |
| Chr3 | exon | 4073867 | 4074238 | 4073867 | ID=MsG0380011722.01.T01:exon:2095;Parent=MsG0380011722.01.T01 |
| Chr3 | CDS | 4073867 | 4074238 | 4073867 | ID=MsG0380011722.01.T01:cds;Parent=MsG0380011722.01.T01 |
| Gene Sequence |
| Protein sequence |
