AlfalfaGEDB | MsG0380012576.01

M. sativa cultivar ZhongmuNo.1 / MsG0380012576.01

KEGG: -
GO: GO:0008270
PFAM: PF13639

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012576.01.T01	AES69216.1	91.729	133	11	0	1	133	21	153	1.84E-85	256

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012576.01.T01	Q6AVN2	36.957	92	50	2	38	126	154	240	2.40E-13	68.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012576.01.T01	G7IZ80	91.729	133	11	0	1	133	21	153	8.79e-86	256

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0180001070.01	MsG0380012576.01	PPI
MsG0580024136.01	MsG0380012576.01	PPI
MsG0480023037.01	MsG0380012576.01	PPI
MsG0580026022.01	MsG0380012576.01	PPI
MsG0580026022.01	MsG0380012576.01	PPI
MsG0580026022.01	MsG0380012576.01	PPI
MsG0180003474.01	MsG0380012576.01	PPI
MsG0380012581.01	MsG0380012576.01	PPI
MsG0580029917.01	MsG0380012576.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012576.01.T01	MTR_3g025510	91.729	133	11	0	1	133	21	153	2.23e-89	256
MsG0380012576.01.T01	MTR_3g025380	51.111	135	51	4	1	132	19	141	1.43e-29	104
MsG0380012576.01.T01	MTR_3g025550	72.222	54	15	0	74	127	6	59	7.52e-24	87.8
MsG0380012576.01.T01	MTR_3g025620	67.857	56	18	0	78	133	1	56	1.89e-22	84.0
MsG0380012576.01.T01	MTR_2g058360	49.462	93	36	3	40	130	58	141	2.03e-19	78.6
MsG0380012576.01.T01	MTR_7g074170	43.373	83	43	2	48	126	304	386	3.70e-17	76.6
MsG0380012576.01.T01	MTR_6g079660	34.737	95	53	3	41	126	182	276	5.67e-15	70.5
MsG0380012576.01.T01	MTR_3g072630	36.275	102	64	1	29	129	164	265	1.80e-13	65.9
MsG0380012576.01.T01	MTR_3g072630	36.275	102	64	1	29	129	164	265	2.01e-13	65.9
MsG0380012576.01.T01	MTR_1g114320	30.208	96	63	2	38	129	143	238	6.69e-13	64.3
MsG0380012576.01.T01	MTR_4g108830	32.331	133	75	4	2	129	69	191	6.99e-13	62.8
MsG0380012576.01.T01	MTR_2g097620	35.938	64	41	0	63	126	167	230	1.32e-12	63.5
MsG0380012576.01.T01	MTR_3g083480	35.294	85	47	2	48	129	202	281	1.52e-12	63.5
MsG0380012576.01.T01	MTR_3g088080	38.462	65	36	2	63	126	18	79	1.61e-12	59.3
MsG0380012576.01.T01	MTR_1g114620	36.559	93	53	2	38	126	215	305	2.58e-12	62.8
MsG0380012576.01.T01	MTR_4g108830	43.750	64	35	1	67	129	156	219	5.48e-12	60.8
MsG0380012576.01.T01	MTR_4g064570	41.176	68	38	2	63	129	159	225	9.02e-12	61.2
MsG0380012576.01.T01	MTR_0067s0100	33.333	90	56	1	41	126	142	231	9.80e-12	60.8
MsG0380012576.01.T01	MTR_5g041330	29.897	97	57	2	38	126	53	146	2.17e-11	59.3
MsG0380012576.01.T01	MTR_2g007340	40.000	65	39	0	69	133	185	249	3.29e-11	59.7
MsG0380012576.01.T01	MTR_5g080350	40.984	61	36	0	69	129	177	237	4.26e-11	59.3
MsG0380012576.01.T01	MTR_3g081030	34.247	73	47	1	58	129	92	164	4.71e-11	58.2
MsG0380012576.01.T01	MTR_1g051105	44.898	49	27	0	85	133	159	207	4.89e-11	58.5
MsG0380012576.01.T01	MTR_5g058530	46.939	49	26	0	78	126	217	265	5.22e-11	58.9
MsG0380012576.01.T01	MTR_5g041310	39.062	64	36	1	63	126	96	156	6.29e-11	58.2
MsG0380012576.01.T01	MTR_4g110100	45.000	60	32	1	68	126	342	401	7.79e-11	58.5
MsG0380012576.01.T01	MTR_7g092960	42.623	61	33	1	68	126	36	96	9.42e-11	55.1
MsG0380012576.01.T01	MTR_4g106960	36.986	73	45	1	57	129	208	279	9.73e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012576.01.T01	AT4G26400	34.951	103	59	2	27	126	185	282	1.94e-14	68.9
MsG0380012576.01.T01	AT4G26400	34.951	103	59	2	27	126	185	282	1.94e-14	68.9
MsG0380012576.01.T01	AT5G08139	46.154	65	34	1	63	126	286	350	2.30e-14	68.6
MsG0380012576.01.T01	AT1G14200	37.333	75	47	0	52	126	76	150	5.72e-14	65.5
MsG0380012576.01.T01	AT3G02340	41.304	92	47	4	41	126	286	376	1.46e-13	66.6
MsG0380012576.01.T01	AT3G02340	41.304	92	47	4	41	126	286	376	1.46e-13	66.6
MsG0380012576.01.T01	AT2G40830	34.653	101	58	4	41	133	138	238	3.59e-13	65.1
MsG0380012576.01.T01	AT2G40830	34.653	101	58	4	41	133	138	238	3.59e-13	65.1
MsG0380012576.01.T01	AT2G40830	34.653	101	58	4	41	133	138	238	3.59e-13	65.1
MsG0380012576.01.T01	AT2G40830	34.653	101	58	4	41	133	138	238	3.59e-13	65.1
MsG0380012576.01.T01	AT3G19950	31.579	95	56	3	41	126	221	315	8.25e-13	64.3
MsG0380012576.01.T01	AT3G19950	31.579	95	56	3	41	126	221	315	8.25e-13	64.3
MsG0380012576.01.T01	AT3G19950	31.579	95	56	3	41	126	163	257	1.01e-12	63.9
MsG0380012576.01.T01	AT3G30460	40.506	79	45	1	54	130	64	142	1.19e-12	61.2
MsG0380012576.01.T01	AT3G30460	46.875	64	32	1	69	130	115	178	1.22e-12	62.0
MsG0380012576.01.T01	AT4G05350	43.939	66	32	2	69	129	139	204	1.65e-12	62.0
MsG0380012576.01.T01	AT5G15820	38.554	83	47	2	48	126	250	332	1.95e-12	63.2
MsG0380012576.01.T01	AT5G54990	53.333	45	21	0	82	126	171	215	2.37e-12	62.0
MsG0380012576.01.T01	AT4G12190	43.939	66	32	2	69	129	4	69	3.00e-12	58.5
MsG0380012576.01.T01	AT5G60820	50.000	46	23	0	81	126	368	413	3.11e-12	62.8
MsG0380012576.01.T01	AT1G60360	29.915	117	71	4	28	133	156	272	3.34e-12	62.4
MsG0380012576.01.T01	AT4G12210	42.424	66	33	2	69	129	135	200	4.37e-12	60.8
MsG0380012576.01.T01	AT3G10815	48.889	45	23	0	85	129	121	165	7.02e-12	60.5
MsG0380012576.01.T01	AT3G60080	38.806	67	33	1	68	126	144	210	7.06e-12	61.2
MsG0380012576.01.T01	AT4G12140	39.506	81	40	3	50	126	121	196	8.11e-12	60.1
MsG0380012576.01.T01	AT5G01980	34.783	92	57	1	38	126	300	391	1.73e-11	60.5
MsG0380012576.01.T01	AT1G18780	44.000	50	28	0	77	126	269	318	1.91e-11	60.5
MsG0380012576.01.T01	AT1G55530	35.938	64	36	1	63	126	206	264	7.52e-11	58.5
MsG0380012576.01.T01	AT1G55530	35.938	64	36	1	63	126	206	264	7.52e-11	58.5
MsG0380012576.01.T01	AT5G56340	31.959	97	58	2	33	126	209	300	8.33e-11	58.5
MsG0380012576.01.T01	AT1G18770	45.238	42	23	0	85	126	59	100	9.11e-11	55.5
MsG0380012576.01.T01	AT2G44330	52.381	42	20	0	85	126	96	137	9.13e-11	57.0
MsG0380012576.01.T01	AT1G55530	35.938	64	36	1	63	126	173	231	9.65e-11	58.2
MsG0380012576.01.T01	AT5G59550	35.938	64	41	0	63	126	282	345	1.00e-10	58.5

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACGACTCGGTCATGATTT+TGG	0.175603	3:-20066639	None:intergenic
TTTCTTGTGAACCTTCTTGA+TGG	0.337595	3:+20066566	None:intergenic
CCTTCTTCAATTTCAACTCT+TGG	0.340786	3:+20066419	None:intergenic
ACGACTACTCCTTATGTTGA+TGG	0.398986	3:-20066527	MsG0380012576.01.T01:CDS
CGAATTGCTTTCGACCCAAC+CGG	0.412863	3:+20066350	None:intergenic
CGGTTGGGTCGAAAGCAATT+CGG	0.413830	3:-20066349	MsG0380012576.01.T01:CDS
TATTTGTTTAGCAGAGCTTC+CGG	0.447189	3:-20066369	MsG0380012576.01.T01:CDS
CCAAGAGTTGAAATTGAAGA+AGG	0.453666	3:-20066419	MsG0380012576.01.T01:CDS
ACGATGAGTGTATCATGAAA+TGG	0.467296	3:-20066304	MsG0380012576.01.T01:CDS
TGATACCATGGAAAATATAG+TGG	0.488151	3:+20066504	None:intergenic
TGTTTAGCAGAGCTTCCGGT+TGG	0.488910	3:-20066365	MsG0380012576.01.T01:CDS
ATATAGTGGCCATCAACATA+AGG	0.494243	3:+20066518	None:intergenic
GTTTAGCAGAGCTTCCGGTT+GGG	0.509547	3:-20066364	MsG0380012576.01.T01:CDS
GATACGCTCTGACGACACAT+AGG	0.570382	3:+20066260	None:intergenic
CGTTGATGTGGTTGATACCA+TGG	0.587395	3:+20066492	None:intergenic
AGAGTGTGATAAAGACGAGG+AGG	0.591123	3:+20066599	None:intergenic
TAGTATGATTCTCGTTGATG+TGG	0.592252	3:+20066480	None:intergenic
TTACTAGAAATCCATCAAGA+AGG	0.594013	3:-20066577	MsG0380012576.01.T01:CDS
ATACGCTCTGACGACACATA+GGG	0.611765	3:+20066261	None:intergenic
TCGTGGTAGATATGCGAGCA+CGG	0.629183	3:+20066323	None:intergenic
GTAAGAGTGTGATAAAGACG+AGG	0.712269	3:+20066596	None:intergenic
ATTTCATGATACACTCATCG+TGG	0.739086	3:+20066306	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TGATACCATGGAAAATATAG+TGG	+	Chr3:20066372-20066391	None:intergenic	30.0%
	TTACTAGAAATCCATCAAGA+AGG	-	Chr3:20066296-20066315	MsG0380012576.01.T01:CDS	30.0%
	ACGATGAGTGTATCATGAAA+TGG	-	Chr3:20066569-20066588	MsG0380012576.01.T01:CDS	35.0%
	ATATAGTGGCCATCAACATA+AGG	+	Chr3:20066358-20066377	None:intergenic	35.0%
	ATTTCATGATACACTCATCG+TGG	+	Chr3:20066570-20066589	None:intergenic	35.0%
	CCAAGAGTTGAAATTGAAGA+AGG	-	Chr3:20066454-20066473	MsG0380012576.01.T01:CDS	35.0%
	CCTTCTTCAATTTCAACTCT+TGG	+	Chr3:20066457-20066476	None:intergenic	35.0%
	TAGTATGATTCTCGTTGATG+TGG	+	Chr3:20066396-20066415	None:intergenic	35.0%
	TATTTGTTTAGCAGAGCTTC+CGG	-	Chr3:20066504-20066523	MsG0380012576.01.T01:CDS	35.0%
	TTTCTTGTGAACCTTCTTGA+TGG	+	Chr3:20066310-20066329	None:intergenic	35.0%
	ACGACTACTCCTTATGTTGA+TGG	-	Chr3:20066346-20066365	MsG0380012576.01.T01:CDS	40.0%
!	GATGGCCACTATATTTTCCA+TGG	-	Chr3:20066364-20066383	MsG0380012576.01.T01:CDS	40.0%
!	GTAAGAGTGTGATAAAGACG+AGG	+	Chr3:20066280-20066299	None:intergenic	40.0%
	AGAGTGTGATAAAGACGAGG+AGG	+	Chr3:20066277-20066296	None:intergenic	45.0%
	ATACGCTCTGACGACACATA+GGG	+	Chr3:20066615-20066634	None:intergenic	45.0%
!	CGTTGATGTGGTTGATACCA+TGG	+	Chr3:20066384-20066403	None:intergenic	45.0%
	CGAATTGCTTTCGACCCAAC+CGG	+	Chr3:20066526-20066545	None:intergenic	50.0%
	CGGTTGGGTCGAAAGCAATT+CGG	-	Chr3:20066524-20066543	MsG0380012576.01.T01:CDS	50.0%
	GATACGCTCTGACGACACAT+AGG	+	Chr3:20066616-20066635	None:intergenic	50.0%
	GTTTAGCAGAGCTTCCGGTT+GGG	-	Chr3:20066509-20066528	MsG0380012576.01.T01:CDS	50.0%
	TCGTGGTAGATATGCGAGCA+CGG	+	Chr3:20066553-20066572	None:intergenic	50.0%
	TGTTTAGCAGAGCTTCCGGT+TGG	-	Chr3:20066508-20066527	MsG0380012576.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	20066247	20066648	20066247	ID=MsG0380012576.01;Name=MsG0380012576.01
Chr3	mRNA	20066247	20066648	20066247	ID=MsG0380012576.01.T01;Parent=MsG0380012576.01;Name=MsG0380012576.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|133
Chr3	exon	20066247	20066648	20066247	ID=MsG0380012576.01.T01:exon:7215;Parent=MsG0380012576.01.T01
Chr3	CDS	20066247	20066648	20066247	ID=MsG0380012576.01.T01:cds;Parent=MsG0380012576.01.T01

Gene Sequence

>MsG0380012576.01.T01

ATGATTTTGGATGCTATTGATCTGAATCCTCCTCGTCTTTATCACACTCTTACTAGAAATCCATCAAGAAGGTTCACAAGAAATATCGTAAGATCTACAACGACTACTCCTTATGTTGATGGCCACTATATTTTCCATGGTATCAACCACATCAACGAGAATCATACTAGTAGAAGAAATAGAGTCAGACATGTTTATCACAATCTTCCAAGAGTTGAAATTGAAGAAGGAATGAAGTGTGAAGCTTTGACGTGCTCTATTTGTTTAGCAGAGCTTCCGGTTGGGTCGAAAGCAATTCGGTTGCCGTGCTCGCATATCTACCACGATGAGTGTATCATGAAATGGTTAGATAGATCGAACACATGCCCTATGTGTCGTCAGAGCGTATCTCATGTTTCTTGA

Protein sequence

>MsG0380012576.01.T01

MILDAIDLNPPRLYHTLTRNPSRRFTRNIVRSTTTTPYVDGHYIFHGINHINENHTSRRNRVRHVYHNLPRVEIEEGMKCEALTCSICLAELPVGSKAIRLPCSHIYHDECIMKWLDRSNTCPMCRQSVSHVS*