AlfalfaGEDB | MsG0380012581.01

M. sativa cultivar ZhongmuNo.1 / MsG0380012581.01

KEGG: -
GO: -
PFAM: PF13639

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012581.01.T01	AES69216.1	87.662	154	18	1	1	153	1	154	5.55E-92	273

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012581.01.T01	Q6DIP3	41.935	62	36	0	88	149	211	272	1.91E-13	69.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012581.01.T01	G7IZ80	87.662	154	18	1	1	153	1	154	2.65e-92	273

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0380012581.01	MsG0580029917.01	PPI
MsG0180001070.01	MsG0380012581.01	PPI
MsG0380012581.01	MsG0580026022.01	PPI
MsG0380012581.01	MsG0580026022.01	PPI
MsG0380012581.01	MsG0580024136.01	PPI
MsG0380012581.01	MsG0580026022.01	PPI
MsG0380012581.01	MsG0180003474.01	PPI
MsG0380012581.01	MsG0480023037.01	PPI
MsG0380012581.01	MsG0380012576.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012581.01.T01	MTR_3g025510	87.662	154	18	1	1	153	1	154	6.72e-96	273
MsG0380012581.01.T01	MTR_3g025380	49.351	154	62	5	1	151	1	141	9.45e-33	113
MsG0380012581.01.T01	MTR_3g025550	74.468	47	12	0	100	146	13	59	2.15e-21	82.0
MsG0380012581.01.T01	MTR_3g025620	67.857	56	18	0	97	152	1	56	2.85e-21	81.6
MsG0380012581.01.T01	MTR_7g074170	39.785	93	52	2	57	145	294	386	7.36e-17	76.6
MsG0380012581.01.T01	MTR_6g079660	32.039	103	66	2	47	145	174	276	1.05e-16	75.9
MsG0380012581.01.T01	MTR_2g058360	47.500	80	36	4	74	149	64	141	1.64e-14	66.6
MsG0380012581.01.T01	MTR_1g114320	28.947	114	77	2	36	145	122	235	3.83e-14	68.6
MsG0380012581.01.T01	MTR_3g088080	48.214	56	25	1	90	145	28	79	4.26e-14	64.3
MsG0380012581.01.T01	MTR_1g114620	35.484	93	54	2	57	145	215	305	6.14e-13	65.1
MsG0380012581.01.T01	MTR_3g072630	36.170	94	59	1	57	149	173	266	6.68e-13	65.1
MsG0380012581.01.T01	MTR_3g072630	36.170	94	59	1	57	149	173	266	7.37e-13	65.1
MsG0380012581.01.T01	MTR_3g083480	32.632	95	56	2	57	148	192	281	2.88e-12	63.2
MsG0380012581.01.T01	MTR_2g097620	38.462	65	38	2	82	145	167	230	3.78e-12	63.2
MsG0380012581.01.T01	MTR_4g064570	43.077	65	35	2	82	145	159	222	5.85e-12	62.4
MsG0380012581.01.T01	MTR_0067s0100	30.693	101	70	0	45	145	131	231	1.00e-11	61.6
MsG0380012581.01.T01	MTR_2g007340	39.394	66	40	0	88	153	185	250	1.53e-11	61.2
MsG0380012581.01.T01	MTR_5g080350	40.984	61	36	0	88	148	177	237	1.72e-11	61.2
MsG0380012581.01.T01	MTR_4g108830	42.188	64	36	1	86	148	128	191	3.00e-11	58.9
MsG0380012581.01.T01	MTR_4g108830	42.188	64	36	1	86	148	156	219	4.06e-11	59.3
MsG0380012581.01.T01	MTR_4g110100	44.262	61	33	1	86	145	341	401	7.17e-11	59.3
MsG0380012581.01.T01	MTR_0186s0090	48.980	49	22	1	103	148	115	163	7.53e-11	57.4
MsG0380012581.01.T01	MTR_5g058530	46.939	49	26	0	97	145	217	265	8.29e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012581.01.T01	AT3G02340	41.758	91	48	3	60	145	286	376	5.81e-15	71.2
MsG0380012581.01.T01	AT3G02340	41.758	91	48	3	60	145	286	376	5.81e-15	71.2
MsG0380012581.01.T01	AT5G15820	37.895	95	52	3	58	145	238	332	8.82e-15	70.5
MsG0380012581.01.T01	AT1G14200	38.667	75	46	0	71	145	76	150	2.22e-14	67.4
MsG0380012581.01.T01	AT4G26400	34.951	103	59	2	46	145	185	282	2.54e-14	69.3
MsG0380012581.01.T01	AT4G26400	34.951	103	59	2	46	145	185	282	2.54e-14	69.3
MsG0380012581.01.T01	AT5G01980	36.957	92	55	1	57	145	300	391	8.49e-14	68.2
MsG0380012581.01.T01	AT3G19950	30.108	93	61	2	57	145	165	257	9.43e-14	67.4
MsG0380012581.01.T01	AT3G19950	30.108	93	61	2	57	145	223	315	1.10e-13	67.4
MsG0380012581.01.T01	AT3G19950	30.108	93	61	2	57	145	223	315	1.10e-13	67.4
MsG0380012581.01.T01	AT5G08139	44.615	65	35	1	82	145	286	350	1.22e-13	67.4
MsG0380012581.01.T01	AT5G54990	55.556	45	20	0	101	145	171	215	3.56e-13	64.7
MsG0380012581.01.T01	AT2G40830	30.275	109	71	3	49	153	132	239	6.79e-13	65.1
MsG0380012581.01.T01	AT2G40830	30.275	109	71	3	49	153	132	239	6.79e-13	65.1
MsG0380012581.01.T01	AT2G40830	30.275	109	71	3	49	153	132	239	6.79e-13	65.1
MsG0380012581.01.T01	AT2G40830	30.275	109	71	3	49	153	132	239	6.79e-13	65.1
MsG0380012581.01.T01	AT1G60360	39.130	69	41	1	86	153	205	273	1.04e-12	64.7
MsG0380012581.01.T01	AT4G05350	39.437	71	40	1	81	148	134	204	4.93e-12	61.6
MsG0380012581.01.T01	AT3G30460	46.032	63	32	1	88	148	115	177	6.10e-12	60.8
MsG0380012581.01.T01	AT3G30460	39.744	78	45	1	73	148	64	141	6.12e-12	60.1
MsG0380012581.01.T01	AT1G18770	43.750	48	27	0	103	150	58	105	6.66e-12	58.9
MsG0380012581.01.T01	AT5G56340	32.609	92	54	2	57	145	214	300	1.26e-11	61.6
MsG0380012581.01.T01	AT3G60080	38.806	67	33	1	87	145	144	210	1.30e-11	61.2
MsG0380012581.01.T01	AT4G12190	41.935	62	33	1	90	148	8	69	2.18e-11	56.6
MsG0380012581.01.T01	AT3G10815	44.681	47	26	0	104	150	121	167	3.81e-11	58.9
MsG0380012581.01.T01	AT4G12210	36.486	74	44	1	78	148	127	200	4.31e-11	58.9
MsG0380012581.01.T01	AT3G46620	40.000	65	37	2	82	145	193	256	6.28e-11	59.7
MsG0380012581.01.T01	AT2G29840	47.826	46	24	0	100	145	240	285	7.40e-11	59.3
MsG0380012581.01.T01	AT2G44330	48.000	50	26	0	104	153	96	145	7.52e-11	57.8
MsG0380012581.01.T01	AT1G55530	28.431	102	61	3	49	145	170	264	7.59e-11	59.3
MsG0380012581.01.T01	AT1G55530	28.431	102	61	3	49	145	170	264	7.59e-11	59.3

Find 25 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACGACTCGGTTATGATTT+TGG	0.133349	3:-20123537	MsG0380012581.01.T01:CDS
GATTTGTTTGGTGGAGCTTT+CGG	0.379375	3:-20123267	MsG0380012581.01.T01:CDS
TCTTCTACAATTTCGACTCT+TGG	0.408499	3:+20123317	None:intergenic
TTTGATGTGTTCGATTTGTT+TGG	0.422388	3:-20123279	MsG0380012581.01.T01:CDS
ACGACTACTCCATATGTTGA+TGG	0.440424	3:-20123425	MsG0380012581.01.T01:CDS
CGGTTGGGTTGAAAGCAATT+CGG	0.442494	3:-20123247	MsG0380012581.01.T01:CDS
ACGATGAATGTATCATGAAA+TGG	0.447486	3:-20123202	MsG0380012581.01.T01:CDS
TGTTTGGTGGAGCTTTCGGT+TGG	0.460170	3:-20123263	MsG0380012581.01.T01:CDS
ATATAGTGGCCATCAACATA+TGG	0.473428	3:+20123416	None:intergenic
GATGTGTTCGATTTGTTTGG+TGG	0.485259	3:-20123276	MsG0380012581.01.T01:CDS
TGGAGCAACATCGTCGTTCA+AGG	0.491831	3:-20123580	MsG0380012581.01.T01:CDS
TTACTAGAAATTCATCAAGA+AGG	0.509458	3:-20123475	MsG0380012581.01.T01:CDS
CTAGTAAAAGTGTGATAATG+AGG	0.517777	3:+20123491	None:intergenic
GTTTGGTGGAGCTTTCGGTT+GGG	0.522058	3:-20123262	MsG0380012581.01.T01:CDS
TGATGACATGTAAAATATAG+TGG	0.527734	3:+20123402	None:intergenic
TAAAAGTGTGATAATGAGGA+GGG	0.542966	3:+20123495	None:intergenic
GATACACTCTGACGACACAT+AGG	0.555715	3:+20123158	None:intergenic
AACCGAGTCGTTGTCTTCAG+CGG	0.564335	3:+20123547	None:intergenic
TGAACGACGATGTTGCTCCA+TGG	0.567207	3:+20123583	None:intergenic
GTAAAAGTGTGATAATGAGG+AGG	0.570510	3:+20123494	None:intergenic
TAGTATGATTCTCGTTGATG+TGG	0.592252	3:+20123378	None:intergenic
ATACACTCTGACGACACATA+GGG	0.607340	3:+20123159	None:intergenic
TCGTGGTAGATATGCGAGCA+AGG	0.609523	3:+20123221	None:intergenic
GTCCGCTGAAGACAACGACT+CGG	0.678762	3:-20123549	MsG0380012581.01.T01:CDS
ATTTCATGATACATTCATCG+TGG	0.701379	3:+20123204	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGATGACATGTAAAATATAG+TGG	+	Chr3:20123324-20123343	None:intergenic	25.0%
!	TTACTAGAAATTCATCAAGA+AGG	-	Chr3:20123248-20123267	MsG0380012581.01.T01:CDS	25.0%
	ACGATGAATGTATCATGAAA+TGG	-	Chr3:20123521-20123540	MsG0380012581.01.T01:CDS	30.0%
	ATTTCATGATACATTCATCG+TGG	+	Chr3:20123522-20123541	None:intergenic	30.0%
!	CTAGTAAAAGTGTGATAATG+AGG	+	Chr3:20123235-20123254	None:intergenic	30.0%
!	TAAAAGTGTGATAATGAGGA+GGG	+	Chr3:20123231-20123250	None:intergenic	30.0%
!	TTTGATGTGTTCGATTTGTT+TGG	-	Chr3:20123444-20123463	MsG0380012581.01.T01:CDS	30.0%
	ATATAGTGGCCATCAACATA+TGG	+	Chr3:20123310-20123329	None:intergenic	35.0%
	TAGTATGATTCTCGTTGATG+TGG	+	Chr3:20123348-20123367	None:intergenic	35.0%
	TCTTCTACAATTTCGACTCT+TGG	+	Chr3:20123409-20123428	None:intergenic	35.0%
!	GTAAAAGTGTGATAATGAGG+AGG	+	Chr3:20123232-20123251	None:intergenic	35.0%
	ACGACTACTCCATATGTTGA+TGG	-	Chr3:20123298-20123317	MsG0380012581.01.T01:CDS	40.0%
	ATACACTCTGACGACACATA+GGG	+	Chr3:20123567-20123586	None:intergenic	40.0%
!	GATGTGTTCGATTTGTTTGG+TGG	-	Chr3:20123447-20123466	MsG0380012581.01.T01:CDS	40.0%
!	GATTTGTTTGGTGGAGCTTT+CGG	-	Chr3:20123456-20123475	MsG0380012581.01.T01:CDS	40.0%
!!!	CAACGACTCGGTTATGATTT+TGG	-	Chr3:20123186-20123205	MsG0380012581.01.T01:CDS	40.0%
	GATACACTCTGACGACACAT+AGG	+	Chr3:20123568-20123587	None:intergenic	45.0%
!!	CGGTTGGGTTGAAAGCAATT+CGG	-	Chr3:20123476-20123495	MsG0380012581.01.T01:CDS	45.0%
	AACCGAGTCGTTGTCTTCAG+CGG	+	Chr3:20123179-20123198	None:intergenic	50.0%
	TCGTGGTAGATATGCGAGCA+AGG	+	Chr3:20123505-20123524	None:intergenic	50.0%
	TGGAGCAACATCGTCGTTCA+AGG	-	Chr3:20123143-20123162	MsG0380012581.01.T01:CDS	50.0%
!	GTTTGGTGGAGCTTTCGGTT+GGG	-	Chr3:20123461-20123480	MsG0380012581.01.T01:CDS	50.0%
!	TGTTTGGTGGAGCTTTCGGT+TGG	-	Chr3:20123460-20123479	MsG0380012581.01.T01:CDS	50.0%
	GTCCGCTGAAGACAACGACT+CGG	-	Chr3:20123174-20123193	MsG0380012581.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	20123142	20123603	20123142	ID=MsG0380012581.01;Name=MsG0380012581.01
Chr3	mRNA	20123142	20123603	20123142	ID=MsG0380012581.01.T01;Parent=MsG0380012581.01;Name=MsG0380012581.01.T01;_AED=0.24;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|153
Chr3	exon	20123142	20123603	20123142	ID=MsG0380012581.01.T01:exon:3718;Parent=MsG0380012581.01.T01
Chr3	CDS	20123142	20123603	20123142	ID=MsG0380012581.01.T01:cds;Parent=MsG0380012581.01.T01

Gene Sequence

>MsG0380012581.01.T01

ATGGAGCAACATCGTCGTTCAAGGAGAAGTGTGTCCGCTGAAGACAACGACTCGGTTATGATTTTGGATGCTATTGATCTAAATCTCCCTCCTCATTATCACACTTTTACTAGAAATTCATCAAGAAGGTTTGCAAGAAATTTTATAAGATCTTCAACGACTACTCCATATGTTGATGGCCACTATATTTTACATGTCATCAACCACATCAACGAGAATCATACTAGTAGAAGAAATAGAGTCAGACATGTTTATCACAATCTTCCAAGAGTCGAAATTGTAGAAGAAATGAAGTGTGAAGCTTTGATGTGTTCGATTTGTTTGGTGGAGCTTTCGGTTGGGTTGAAAGCAATTCGGTTGCCTTGCTCGCATATCTACCACGATGAATGTATCATGAAATGGTTAGATAGATCAAACACATGCCCTATGTGTCGTCAGAGTGTATCTCATGTTTCTTCATGA

Protein sequence

>MsG0380012581.01.T01

MEQHRRSRRSVSAEDNDSVMILDAIDLNLPPHYHTFTRNSSRRFARNFIRSSTTTPYVDGHYILHVINHINENHTSRRNRVRHVYHNLPRVEIVEEMKCEALMCSICLVELSVGLKAIRLPCSHIYHDECIMKWLDRSNTCPMCRQSVSHVSS*