AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023037.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023037.01.T01 MTR_8g085510 76.772 254 29 2 72 299 1 250 3.43e-104 311
MsG0480023037.01.T01 MTR_8g085510 36.979 192 53 5 210 363 58 219 1.79e-11 65.1
MsG0480023037.01.T01 MTR_8g085530 57.500 360 57 11 69 403 8 296 4.84e-86 264
MsG0480023037.01.T01 MTR_8g102170 45.205 146 60 8 1 134 1 138 1.05e-22 93.6
MsG0480023037.01.T01 MTR_5g037840 46.667 105 44 4 305 405 35 131 4.92e-19 83.2
MsG0480023037.01.T01 MTR_5g006240 53.488 86 30 3 324 405 147 226 1.29e-18 84.7
MsG0480023037.01.T01 MTR_5g037810 45.714 105 45 3 305 405 35 131 1.54e-18 81.6
MsG0480023037.01.T01 MTR_8g085550 39.231 130 56 6 288 405 32 150 2.67e-15 73.2
MsG0480023037.01.T01 MTR_8g085520 46.392 97 36 5 315 405 64 150 5.99e-15 72.8
MsG0480023037.01.T01 MTR_1g029860 31.646 158 77 8 259 406 43 179 2.04e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATATTGATCTACAAAATA+TGG 0.206665 4:+80027963 MsG0480023037.01.T01:CDS
GAATTGGAAGGTTATCATTT+TGG 0.222996 4:+80027925 MsG0480023037.01.T01:CDS
TCAATTTGTTTGAAGAAGTA+TGG 0.250205 4:+80028165 MsG0480023037.01.T01:CDS
GATAGGGTTTGGGTTGATAT+TGG 0.284097 4:-80027141 None:intergenic
AATGATCTTCTTCCAATTCC+TGG 0.291890 4:-80027831 None:intergenic
TGATGATTTGCTAGAGGAAT+TGG 0.295980 4:+80027792 MsG0480023037.01.T01:CDS
TGATGATTTGCTAGAGGAAT+TGG 0.295980 4:+80028008 MsG0480023037.01.T01:CDS
AATGTCGTACAAGTTGAAAT+AGG 0.301859 4:-80027231 None:intergenic
TGAGGATTTGCTAGATGAAT+TGG 0.302987 4:+80027457 MsG0480023037.01.T01:CDS
TGAATTGCTACTGGAATTGC+AGG 0.310631 4:+80027514 MsG0480023037.01.T01:CDS
TGTGGATTTGCTAGAGGAAT+TGG 0.320439 4:+80027687 MsG0480023037.01.T01:CDS
TATGGATTTGCTAGATCAAT+TGG 0.325127 4:+80027981 MsG0480023037.01.T01:CDS
TGATGATTTGCTGGAGGAAT+TGG 0.326901 4:+80028104 MsG0480023037.01.T01:CDS
TGTGGATTTGCTAGATCAAT+TGG 0.350799 4:+80027765 MsG0480023037.01.T01:CDS
AGATGACTCACTCCAGGAAT+TGG 0.361176 4:+80027819 MsG0480023037.01.T01:CDS
TGATGATTTGCTACATGAAT+TGG 0.364465 4:+80027484 MsG0480023037.01.T01:CDS
ATGAAGTAGTCATCGATGAT+AGG 0.379738 4:-80027158 None:intergenic
TGTGGATTTGCTAGATCAAA+TGG 0.412595 4:+80027882 MsG0480023037.01.T01:CDS
TGTAGATCAATATAATTATG+AGG 0.419977 4:-80027955 None:intergenic
CGTTGAAGGACGTGACATAT+TGG 0.420311 4:+80027346 MsG0480023037.01.T01:CDS
AGTCATCGATGATAGGGTTT+GGG 0.420451 4:-80027151 None:intergenic
TTGTTGATCATATAATTATG+AGG 0.422618 4:-80027557 None:intergenic
TAGTCATCGATGATAGGGTT+TGG 0.428496 4:-80027152 None:intergenic
TTACGATGAGATTGCTCAAA+AGG 0.431045 4:-80027416 None:intergenic
TGTTGATCATTAGAAATATG+AGG 0.432796 4:-80027739 None:intergenic
TTTGTTGATCATTATCATCA+TGG 0.434334 4:-80028053 None:intergenic
AAGAGATTGTGCTAAAGAAA+TGG 0.444674 4:+80027313 MsG0480023037.01.T01:CDS
GTATGCGAGTCGACACCCTT+TGG 0.447302 4:-80027256 None:intergenic
ATGTCGTACAAGTTGAAATA+GGG 0.452084 4:-80027230 None:intergenic
ATGGTCAGATGATGATTTGC+TGG 0.455619 4:+80028095 MsG0480023037.01.T01:CDS
ATAGGTCCGGTTGATTCTAT+AGG 0.456504 4:-80027041 None:intergenic
ATGGTTGCTTTGAACGTTGA+AGG 0.469698 4:+80027332 MsG0480023037.01.T01:CDS
GGAAGAAAATGAATTGCTAC+TGG 0.476761 4:+80027505 MsG0480023037.01.T01:CDS
ATTCTTGCCCATTGTGTCGA+CGG 0.488991 4:+80028274 MsG0480023037.01.T01:CDS
TTATCTCCCGTCGACACAAT+GGG 0.496515 4:-80028281 None:intergenic
TTCTAATGATCAACAAAATG+TGG 0.506114 4:+80027747 MsG0480023037.01.T01:CDS
ATTAAAATTGCATAAGATCT+CGG 0.511987 4:-80027189 None:intergenic
CAAGGAATGTTTGATGAGAT+AGG 0.511992 4:-80027059 None:intergenic
TTCTTGCCCATTGTGTCGAC+GGG 0.513644 4:+80028275 MsG0480023037.01.T01:CDS
GTGACTCCTATAGAATCAAC+CGG 0.524107 4:+80027035 MsG0480023037.01.T01:CDS
TGAAGTAGTCATCGATGATA+GGG 0.541858 4:-80027157 None:intergenic
AATGTTTGATGAGATAGGTC+CGG 0.543322 4:-80027054 None:intergenic
TTTATCTCCCGTCGACACAA+TGG 0.550616 4:-80028282 None:intergenic
ATTCTATAGGAGTCACACTT+AGG 0.554972 4:-80027028 None:intergenic
GGAAGAAGATGACTCACTCC+AGG 0.558883 4:+80027813 MsG0480023037.01.T01:CDS
TTTAGTGTAAATGAAACGAA+TGG 0.562355 4:-80027099 None:intergenic
CAAGTTGAAATAGGGATCCA+TGG 0.566294 4:-80027222 None:intergenic
TTATAATGATCAACAAAATG+TGG 0.566948 4:+80027864 MsG0480023037.01.T01:CDS
TTATAATGATCAACAAAGTG+TGG 0.579978 4:+80027669 MsG0480023037.01.T01:CDS
AAATTGCATAAGATCTCGGA+TGG 0.583718 4:-80027185 None:intergenic
ATGATGATTTGCTAGAGGAA+TGG 0.586544 4:+80028076 MsG0480023037.01.T01:CDS
AGGAGTCACACTTAGGCTGA+AGG 0.605015 4:-80027021 None:intergenic
GGAAGATGATGATTTGCTAG+AGG 0.609396 4:+80027786 MsG0480023037.01.T01:CDS
GGAAGATGATGATTTGCTAG+AGG 0.609396 4:+80028002 MsG0480023037.01.T01:CDS
ACAAAGTGTGGATTTGCTAG+AGG 0.615916 4:+80027681 MsG0480023037.01.T01:CDS
ACAAAATGATGATTTGCTAG+AGG 0.617422 4:+80028071 MsG0480023037.01.T01:CDS
AGTCACACTTAGGCTGAAGG+TGG 0.638915 4:-80027018 None:intergenic
GAGATGATATCTTTAAAACA+AGG 0.652430 4:-80027077 None:intergenic
ACTGTGTCTAGTATGTGAGT+CGG 0.672246 4:-80027278 None:intergenic
GTCAGATGATGATTTGCTGG+AGG 0.692347 4:+80028098 MsG0480023037.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATTGATCTACAAAATA+TGG + Chr4:80027963-80027982 MsG0480023037.01.T01:CDS 15.0%
!! ATTAAAATTGCATAAGATCT+CGG - Chr4:80027192-80027211 None:intergenic 20.0%
!! ATTATATGATCAACAAAGTT+TGG + Chr4:80027564-80027583 MsG0480023037.01.T01:intron 20.0%
!! TGTAGATCAATATAATTATG+AGG - Chr4:80027958-80027977 None:intergenic 20.0%
!! TTATAATGATCAACAAAATG+TGG + Chr4:80027864-80027883 MsG0480023037.01.T01:CDS 20.0%
!! TTGTTGATCATATAATTATG+AGG - Chr4:80027560-80027579 None:intergenic 20.0%
! TGTTGATCATTAGAAATATG+AGG - Chr4:80027742-80027761 None:intergenic 25.0%
! TTATAATGATCAACAAAGTG+TGG + Chr4:80027669-80027688 MsG0480023037.01.T01:CDS 25.0%
! TTCTAATGATCAACAAAATG+TGG + Chr4:80027747-80027766 MsG0480023037.01.T01:CDS 25.0%
! TTTAGTGTAAATGAAACGAA+TGG - Chr4:80027102-80027121 None:intergenic 25.0%
! TTTGTTGATCATTATCATCA+TGG - Chr4:80028056-80028075 None:intergenic 25.0%
!! ATAAAAAGTGTATTAGCAAG+TGG + Chr4:80028232-80028251 MsG0480023037.01.T01:CDS 25.0%
!! TCAATTTGTTTGAAGAAGTA+TGG + Chr4:80028165-80028184 MsG0480023037.01.T01:CDS 25.0%
!! TGATGATTTTCTACAAGAAT+TGG + Chr4:80027909-80027928 MsG0480023037.01.T01:CDS 25.0%
!! TTTTCATGATCAACAAAATG+AGG + Chr4:80027439-80027458 MsG0480023037.01.T01:CDS 25.0%
!!! AGATAATGATGATTTGTTAG+AGG + Chr4:80027606-80027625 MsG0480023037.01.T01:intron 25.0%
!!! GAGATGATATCTTTAAAACA+AGG - Chr4:80027080-80027099 None:intergenic 25.0%
AAGAGATTGTGCTAAAGAAA+TGG + Chr4:80027313-80027332 MsG0480023037.01.T01:CDS 30.0%
TCATTATCTTCCAATTGATC+TGG - Chr4:80027595-80027614 None:intergenic 30.0%
! AATGTCGTACAAGTTGAAAT+AGG - Chr4:80027234-80027253 None:intergenic 30.0%
! ATGTCGTACAAGTTGAAATA+GGG - Chr4:80027233-80027252 None:intergenic 30.0%
! GAATTGGAAGGTTATCATTT+TGG + Chr4:80027925-80027944 MsG0480023037.01.T01:CDS 30.0%
! GATTTTCTACAAGAATTGGA+AGG + Chr4:80027913-80027932 MsG0480023037.01.T01:CDS 30.0%
!! ACAAAATGATGATTTGCTAG+AGG + Chr4:80028071-80028090 MsG0480023037.01.T01:CDS 30.0%
!! TATGGATTTGCTAGATCAAT+TGG + Chr4:80027981-80028000 MsG0480023037.01.T01:CDS 30.0%
!! TGATGATTTGCTACATGAAT+TGG + Chr4:80027484-80027503 MsG0480023037.01.T01:CDS 30.0%
!! TGATGATTTGTTAGAGGAAT+TGG + Chr4:80027612-80027631 MsG0480023037.01.T01:intron 30.0%
AAATTGCATAAGATCTCGGA+TGG - Chr4:80027188-80027207 None:intergenic 35.0%
AATGATCTTCTTCCAATTCC+TGG - Chr4:80027834-80027853 None:intergenic 35.0%
AATGTTTGATGAGATAGGTC+CGG - Chr4:80027057-80027076 None:intergenic 35.0%
ATGAAGTAGTCATCGATGAT+AGG - Chr4:80027161-80027180 None:intergenic 35.0%
ATTCTATAGGAGTCACACTT+AGG - Chr4:80027031-80027050 None:intergenic 35.0%
CAAGGAATGTTTGATGAGAT+AGG - Chr4:80027062-80027081 None:intergenic 35.0%
GGAAGAAAATGAATTGCTAC+TGG + Chr4:80027505-80027524 MsG0480023037.01.T01:CDS 35.0%
TGAAGTAGTCATCGATGATA+GGG - Chr4:80027160-80027179 None:intergenic 35.0%
TTACGATGAGATTGCTCAAA+AGG - Chr4:80027419-80027438 None:intergenic 35.0%
TTTGGATTTGCCAGATCAAT+TGG + Chr4:80027582-80027601 MsG0480023037.01.T01:intron 35.0%
! ACTTGTACGACATTTTCCAA+AGG + Chr4:80027240-80027259 MsG0480023037.01.T01:CDS 35.0%
! CTTGTACGACATTTTCCAAA+GGG + Chr4:80027241-80027260 MsG0480023037.01.T01:CDS 35.0%
!! ATGATGATTTGCTAGAGGAA+TGG + Chr4:80027791-80027810 MsG0480023037.01.T01:CDS 35.0%
!! TGAGGATTTGCTAGATGAAT+TGG + Chr4:80027457-80027476 MsG0480023037.01.T01:CDS 35.0%
!! TGATGATTTGCTAGAGGAAT+TGG + Chr4:80027792-80027811 MsG0480023037.01.T01:CDS 35.0%
!! TGTGGATTTGCTAGATCAAA+TGG + Chr4:80027882-80027901 MsG0480023037.01.T01:CDS 35.0%
!! TGTGGATTTGCTAGATCAAT+TGG + Chr4:80027765-80027784 MsG0480023037.01.T01:CDS 35.0%
ACTGTGTCTAGTATGTGAGT+CGG - Chr4:80027281-80027300 None:intergenic 40.0%
AGTCATCGATGATAGGGTTT+GGG - Chr4:80027154-80027173 None:intergenic 40.0%
GTGACTCCTATAGAATCAAC+CGG + Chr4:80027035-80027054 MsG0480023037.01.T01:CDS 40.0%
TAGTCATCGATGATAGGGTT+TGG - Chr4:80027155-80027174 None:intergenic 40.0%
TGAATTGCTACTGGAATTGC+AGG + Chr4:80027514-80027533 MsG0480023037.01.T01:CDS 40.0%
! ATAGGTCCGGTTGATTCTAT+AGG - Chr4:80027044-80027063 None:intergenic 40.0%
! ATGGTCAGATGATGATTTGC+TGG + Chr4:80028095-80028114 MsG0480023037.01.T01:CDS 40.0%
! ATGGTTGCTTTGAACGTTGA+AGG + Chr4:80027332-80027351 MsG0480023037.01.T01:CDS 40.0%
! CAAGTTGAAATAGGGATCCA+TGG - Chr4:80027225-80027244 None:intergenic 40.0%
! TTTTAATGCACACGCAACCA+TGG + Chr4:80027205-80027224 MsG0480023037.01.T01:CDS 40.0%
!! ACAAAGTGTGGATTTGCTAG+AGG + Chr4:80027681-80027700 MsG0480023037.01.T01:CDS 40.0%
!! GATAGGGTTTGGGTTGATAT+TGG - Chr4:80027144-80027163 None:intergenic 40.0%
!! GGAAGATGATGATTTGCTAG+AGG + Chr4:80027786-80027805 MsG0480023037.01.T01:CDS 40.0%
!! TGATGATTTGCTGGAGGAAT+TGG + Chr4:80028104-80028123 MsG0480023037.01.T01:CDS 40.0%
!! TGTGGATTTGCTAGAGGAAT+TGG + Chr4:80027687-80027706 MsG0480023037.01.T01:CDS 40.0%
AGATGACTCACTCCAGGAAT+TGG + Chr4:80027819-80027838 MsG0480023037.01.T01:CDS 45.0%
ATTCTTGCCCATTGTGTCGA+CGG + Chr4:80028274-80028293 MsG0480023037.01.T01:CDS 45.0%
CGTTGAAGGACGTGACATAT+TGG + Chr4:80027346-80027365 MsG0480023037.01.T01:CDS 45.0%
TTATCTCCCGTCGACACAAT+GGG - Chr4:80028284-80028303 None:intergenic 45.0%
TTTATCTCCCGTCGACACAA+TGG - Chr4:80028285-80028304 None:intergenic 45.0%
!! GTCAGATGATGATTTGCTGG+AGG + Chr4:80028098-80028117 MsG0480023037.01.T01:CDS 45.0%
AGGAGTCACACTTAGGCTGA+AGG - Chr4:80027024-80027043 None:intergenic 50.0%
AGTCACACTTAGGCTGAAGG+TGG - Chr4:80027021-80027040 None:intergenic 50.0%
GGAAGAAGATGACTCACTCC+AGG + Chr4:80027813-80027832 MsG0480023037.01.T01:CDS 50.0%
! TTCTTGCCCATTGTGTCGAC+GGG + Chr4:80028275-80028294 MsG0480023037.01.T01:CDS 50.0%
GTATGCGAGTCGACACCCTT+TGG - Chr4:80027259-80027278 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 80027011 80028308 80027011 ID=MsG0480023037.01;Name=MsG0480023037.01
Chr4 mRNA 80027011 80028308 80027011 ID=MsG0480023037.01.T01;Parent=MsG0480023037.01;Name=MsG0480023037.01.T01;_AED=0.56;_eAED=0.57;_QI=0|0|0|1|0|0|2|0|406
Chr4 exon 80027011 80027580 80027011 ID=MsG0480023037.01.T01:exon:11185;Parent=MsG0480023037.01.T01
Chr4 exon 80027658 80028308 80027658 ID=MsG0480023037.01.T01:exon:11186;Parent=MsG0480023037.01.T01
Chr4 CDS 80027011 80027580 80027011 ID=MsG0480023037.01.T01:cds;Parent=MsG0480023037.01.T01
Chr4 CDS 80027658 80028308 80027658 ID=MsG0480023037.01.T01:cds;Parent=MsG0480023037.01.T01
Gene Sequence

>MsG0480023037.01.T01

ATGGATTCCACCTTCAGCCTAAGTGTGACTCCTATAGAATCAACCGGACCTATCTCATCAAACATTCCTTGTTTTAAAGATATCATCTCCATTCGTTTCATTTACACTAAATCAACTTTTTGTTCTACATCCAATATCAACCCAAACCCTATCATCGATGACTACTTCATGTTTCCATCCGAGATCTTATGCAATTTTAATGCACACGCAACCATGGATCCCTATTTCAACTTGTACGACATTTTCCAAAGGGTGTCGACTCGCATACCGACTCACATACTAGACACAGTTCTATATCGCATAAGAGATTGTGCTAAAGAAATGGTTGCTTTGAACGTTGAAGGACGTGACATATTGGAGATGAAAGTGTCACTTCATGTTAACTCAGACATTGAAGAAGATTATCCTTTTGAGCAATCTCATCGTAATTTTCATGATCAACAAAATGAGGATTTGCTAGATGAATTGGAAGATGATGATTTGCTACATGAATTGGAAGAAAATGAATTGCTACTGGAATTGCAGGAAGATCATTTTGAGCAGAATCCTCATAATTATATGATCAACAAATATCTTCGTAATTATAATGATCAACAAAGTGTGGATTTGCTAGAGGAATTGGAAGATGATGATTTTCTACATGAGCAGTATCCTCATATTTCTAATGATCAACAAAATGTGGATTTGCTAGATCAATTGGAAGATGATGATTTGCTAGAGGAATTGGAAGAAGATGACTCACTCCAGGAATTGGAAGAAGATCATTTTGAGCATAATTATAATGATCAACAAAATGTGGATTTGCTAGATCAAATGGAAGATGATGATTTTCTACAAGAATTGGAAGGTTATCATTTTGGAGAGTATCCTCATAATTATATTGATCTACAAAATATGGATTTGCTAGATCAATTGGAAGATGATGATTTGCTAGAGGAATTGGAAGATCATTTTGAGCAATATCTCCATGATGATAATGATCAACAAAATGATGATTTGCTAGAGGAATGGTCAGATGATGATTTGCTGGAGGAATTGGAAGATGATTCTATTTCTTCAGATTCTATAGATCGATGTTCAATTTGTTTGAAGAAGTATGGATCAGAGAAAGAGCTCTTTCATACAAAATGCTCTCATGTTTTTCATAAAAAGTGTATTAGCAAGTGGATTGATTGCTCATCTTCTTATTCTTGCCCATTGTGTCGACGGGAGATAAAATGA
Protein sequence

>MsG0480023037.01.T01

MDSTFSLSVTPIESTGPISSNIPCFKDIISIRFIYTKSTFCSTSNINPNPIIDDYFMFPSEILCNFNAHATMDPYFNLYDIFQRVSTRIPTHILDTVLYRIRDCAKEMVALNVEGRDILEMKVSLHVNSDIEEDYPFEQSHRNFHDQQNEDLLDELEDDDLLHELEENELLLELQEDHFEQNPHNYMINKYLRNYNDQQSVDLLEELEDDDFLHEQYPHISNDQQNVDLLDQLEDDDLLEELEEDDSLQELEEDHFEHNYNDQQNVDLLDQMEDDDFLQELEGYHFGEYPHNYIDLQNMDLLDQLEDDDLLEELEDHFEQYLHDDNDQQNDDLLEEWSDDDLLEELEDDSISSDSIDRCSICLKKYGSEKELFHTKCSHVFHKKCISKWIDCSSSYSCPLCRREIK*