Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013117.01.T01 | KEH33317.1 | 71.739 | 92 | 25 | 1 | 1 | 91 | 1 | 92 | 2.20E-40 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012184.01 | MsG0380013117.01 | 0.808514 | 2.983388e-50 | 1.709240e-47 |
| MsG0380013117.01 | MsG0380014727.01 | 0.811813 | 5.801836e-51 | 3.627140e-48 |
| MsG0380013117.01 | MsG0380015637.01 | 0.811681 | 6.200657e-51 | 3.862613e-48 |
| MsG0380013117.01 | MsG0380015745.01 | 0.803712 | 3.058892e-49 | 1.547949e-46 |
| MsG0380013117.01 | MsG0380016740.01 | 0.815894 | 7.315309e-52 | 5.106683e-49 |
| MsG0380013117.01 | MsG0580024829.01 | 0.807011 | 6.224583e-50 | 3.428711e-47 |
| MsG0280006669.01 | MsG0380013117.01 | 0.833550 | 4.999575e-56 | 5.777181e-53 |
| MsG0280008932.01 | MsG0380013117.01 | 0.833329 | 5.676687e-56 | 6.516762e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013117.01.T01 | MTR_3g435530 | 71.739 | 92 | 25 | 1 | 1 | 91 | 1 | 92 | 2.66e-44 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATTGATAATAAAGATTAT+TGG | 0.223517 | 3:-30732142 | None:intergenic |
| CTTTATTATCAATGATTTAC+AGG | 0.224575 | 3:+30732150 | MsG0380013117.01.T01:CDS |
| TAGTTGTATTTACTTGTAAT+TGG | 0.232881 | 3:-30731710 | None:intergenic |
| CGTTGTTGAAAGGATGGATT+TGG | 0.274923 | 3:+30732216 | MsG0380013117.01.T01:CDS |
| ACATGCAGAAATCATGGATA+TGG | 0.374235 | 3:-30732292 | None:intergenic |
| TGATCGTCAATGGGAGAAAC+TGG | 0.408803 | 3:+30732176 | MsG0380013117.01.T01:CDS |
| CTCCATGACTACCCACCATC+AGG | 0.414784 | 3:+30732241 | MsG0380013117.01.T01:CDS |
| TACCCACCATCAGGGGCTAA+TGG | 0.427754 | 3:+30732250 | MsG0380013117.01.T01:CDS |
| CCCCTGATGGTGGGTAGTCA+TGG | 0.436791 | 3:-30732243 | None:intergenic |
| TTATTTAACAAACAAGCTCT+TGG | 0.449495 | 3:-30731597 | None:intergenic |
| AATCATGGATATGGTACTCT+TGG | 0.463173 | 3:-30732283 | None:intergenic |
| TTACAGGATATGATCGTCAA+TGG | 0.506149 | 3:+30732166 | MsG0380013117.01.T01:CDS |
| GCCTACCGTTGTTGAAAGGA+TGG | 0.525558 | 3:+30732210 | MsG0380013117.01.T01:CDS |
| TACAGGATATGATCGTCAAT+GGG | 0.528990 | 3:+30732167 | MsG0380013117.01.T01:CDS |
| CTGCTTTGAAGCACACAAGA+TGG | 0.535357 | 3:-30731685 | None:intergenic |
| CGACCATTAGCCCCTGATGG+TGG | 0.539870 | 3:-30732253 | None:intergenic |
| TCCATGACTACCCACCATCA+GGG | 0.570592 | 3:+30732242 | MsG0380013117.01.T01:CDS |
| TGCTTTGAAGCACACAAGAT+GGG | 0.575471 | 3:-30731684 | None:intergenic |
| GACCATTAGCCCCTGATGGT+GGG | 0.583752 | 3:-30732252 | None:intergenic |
| ATCTAAGTGAGAAGAATGAA+AGG | 0.584973 | 3:+30731623 | MsG0380013117.01.T01:exon |
| TGGGAGAAACTGGCCGCAAG+AGG | 0.594048 | 3:+30732186 | MsG0380013117.01.T01:CDS |
| AGGAGCCTACCGTTGTTGAA+AGG | 0.595503 | 3:+30732206 | MsG0380013117.01.T01:CDS |
| CAAATCCATCCTTTCAACAA+CGG | 0.623841 | 3:-30732215 | None:intergenic |
| TCCATCCTTTCAACAACGGT+AGG | 0.638932 | 3:-30732211 | None:intergenic |
| CCATGACTACCCACCATCAG+GGG | 0.674593 | 3:+30732243 | MsG0380013117.01.T01:CDS |
| TGACGACCATTAGCCCCTGA+TGG | 0.686530 | 3:-30732256 | None:intergenic |
| ACAACGGTAGGCTCCTCTTG+CGG | 0.691443 | 3:-30732199 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACAAATATATTATATCCTA+AGG | + | Chr3:30731898-30731917 | MsG0380013117.01.T01:intron | 15.0% |
| !!! | TCATTGATAATAAAGATTAT+TGG | - | Chr3:30732145-30732164 | None:intergenic | 15.0% |
| !! | CTTTATTATCAATGATTTAC+AGG | + | Chr3:30732150-30732169 | MsG0380013117.01.T01:CDS | 20.0% |
| !! | TAGTTGTATTTACTTGTAAT+TGG | - | Chr3:30731713-30731732 | None:intergenic | 20.0% |
| !! | TATAATATATTTGTACCTTG+TGG | - | Chr3:30731893-30731912 | None:intergenic | 20.0% |
| !!! | AAAATCTTGTTTCTATAAAC+AGG | + | Chr3:30731810-30731829 | MsG0380013117.01.T01:intron | 20.0% |
| !!! | ACTTCATTTCTTTAAAAACT+TGG | + | Chr3:30731746-30731765 | MsG0380013117.01.T01:intron | 20.0% |
| !!! | ACTTGTTTGTGATTTTTTTA+GGG | + | Chr3:30731657-30731676 | MsG0380013117.01.T01:CDS | 20.0% |
| !!! | ATAGAAACAAGATTTTGTTA+AGG | - | Chr3:30731806-30731825 | None:intergenic | 20.0% |
| ! | TTATTTAACAAACAAGCTCT+TGG | - | Chr3:30731600-30731619 | None:intergenic | 25.0% |
| !!! | CAACCATTGTTTTAAAAAGA+TGG | - | Chr3:30732107-30732126 | None:intergenic | 25.0% |
| !!! | CACTTGTTTGTGATTTTTTT+AGG | + | Chr3:30731656-30731675 | MsG0380013117.01.T01:CDS | 25.0% |
| ACGGTTTAAATCGTAAATGT+TGG | + | Chr3:30732081-30732100 | MsG0380013117.01.T01:intron | 30.0% | |
| ATCTAAGTGAGAAGAATGAA+AGG | + | Chr3:30731623-30731642 | MsG0380013117.01.T01:exon | 30.0% | |
| ATTGCGATTTGAGCAATTAA+TGG | - | Chr3:30731924-30731943 | None:intergenic | 30.0% | |
| !!! | TGGCCATCTTTTTAAAACAA+TGG | + | Chr3:30732101-30732120 | MsG0380013117.01.T01:intron | 30.0% |
| ATTTAAACCGTGACATCAAG+AGG | - | Chr3:30732072-30732091 | None:intergenic | 35.0% | |
| CAAATCCATCCTTTCAACAA+CGG | - | Chr3:30732218-30732237 | None:intergenic | 35.0% | |
| GAATTTGAGCACGTTGTATA+AGG | - | Chr3:30731845-30731864 | None:intergenic | 35.0% | |
| TACAGGATATGATCGTCAAT+GGG | + | Chr3:30732167-30732186 | MsG0380013117.01.T01:CDS | 35.0% | |
| TATTTGTACCTTGTGGTTGT+TGG | - | Chr3:30731886-30731905 | None:intergenic | 35.0% | |
| TGAGATAAGGGTTAAAGAGT+TGG | - | Chr3:30732027-30732046 | None:intergenic | 35.0% | |
| TTACAGGATATGATCGTCAA+TGG | + | Chr3:30732166-30732185 | MsG0380013117.01.T01:CDS | 35.0% | |
| TTTGAGCAATTAATGGCCTT+AGG | - | Chr3:30731917-30731936 | None:intergenic | 35.0% | |
| !! | GTTCAACTGGTTTGAGATAA+GGG | - | Chr3:30732039-30732058 | None:intergenic | 35.0% |
| AATTGCTCAAATCGCAATCG+TGG | + | Chr3:30731926-30731945 | MsG0380013117.01.T01:intron | 40.0% | |
| GATAAGGGTTAAAGAGTTGG+AGG | - | Chr3:30732024-30732043 | None:intergenic | 40.0% | |
| ! | CGTTGTTGAAAGGATGGATT+TGG | + | Chr3:30732216-30732235 | MsG0380013117.01.T01:CDS | 40.0% |
| !! | GGTTCAACTGGTTTGAGATA+AGG | - | Chr3:30732040-30732059 | None:intergenic | 40.0% |
| !! | TGCTTTGAAGCACACAAGAT+GGG | - | Chr3:30731687-30731706 | None:intergenic | 40.0% |
| AGAGAATGCCAACAACCACA+AGG | + | Chr3:30731875-30731894 | MsG0380013117.01.T01:intron | 45.0% | |
| TAAACCGTGACATCAAGAGG+AGG | - | Chr3:30732069-30732088 | None:intergenic | 45.0% | |
| TCCATCCTTTCAACAACGGT+AGG | - | Chr3:30732214-30732233 | None:intergenic | 45.0% | |
| TGATCGTCAATGGGAGAAAC+TGG | + | Chr3:30732176-30732195 | MsG0380013117.01.T01:CDS | 45.0% | |
| !! | CTGCTTTGAAGCACACAAGA+TGG | - | Chr3:30731688-30731707 | None:intergenic | 45.0% |
| AGGAGCCTACCGTTGTTGAA+AGG | + | Chr3:30732206-30732225 | MsG0380013117.01.T01:CDS | 50.0% | |
| CCAACCTCCTCTTGATGTCA+CGG | + | Chr3:30732062-30732081 | MsG0380013117.01.T01:intron | 50.0% | |
| CGTGACATCAAGAGGAGGTT+GGG | - | Chr3:30732064-30732083 | None:intergenic | 50.0% | |
| GCCTACCGTTGTTGAAAGGA+TGG | + | Chr3:30732210-30732229 | MsG0380013117.01.T01:CDS | 50.0% | |
| TCCATGACTACCCACCATCA+GGG | + | Chr3:30732242-30732261 | MsG0380013117.01.T01:CDS | 50.0% | |
| ACAACGGTAGGCTCCTCTTG+CGG | - | Chr3:30732202-30732221 | None:intergenic | 55.0% | |
| AGGAGGTTGGGTGGTTCAAC+TGG | - | Chr3:30732052-30732071 | None:intergenic | 55.0% | |
| CCATGACTACCCACCATCAG+GGG | + | Chr3:30732243-30732262 | MsG0380013117.01.T01:CDS | 55.0% | |
| CCGTGACATCAAGAGGAGGT+TGG | - | Chr3:30732065-30732084 | None:intergenic | 55.0% | |
| CTCCATGACTACCCACCATC+AGG | + | Chr3:30732241-30732260 | MsG0380013117.01.T01:CDS | 55.0% | |
| GACATCAAGAGGAGGTTGGG+TGG | - | Chr3:30732061-30732080 | None:intergenic | 55.0% | |
| GACCATTAGCCCCTGATGGT+GGG | - | Chr3:30732255-30732274 | None:intergenic | 55.0% | |
| TACCCACCATCAGGGGCTAA+TGG | + | Chr3:30732250-30732269 | MsG0380013117.01.T01:CDS | 55.0% | |
| TGACGACCATTAGCCCCTGA+TGG | - | Chr3:30732259-30732278 | None:intergenic | 55.0% | |
| CGACCATTAGCCCCTGATGG+TGG | - | Chr3:30732256-30732275 | None:intergenic | 60.0% | |
| TGGGAGAAACTGGCCGCAAG+AGG | + | Chr3:30732186-30732205 | MsG0380013117.01.T01:CDS | 60.0% | |
| !! | CCCCTGATGGTGGGTAGTCA+TGG | - | Chr3:30732246-30732265 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 30731590 | 30732303 | 30731590 | ID=MsG0380013117.01;Name=MsG0380013117.01 |
| Chr3 | mRNA | 30731590 | 30732303 | 30731590 | ID=MsG0380013117.01.T01;Parent=MsG0380013117.01;Name=MsG0380013117.01.T01;_AED=0.39;_eAED=0.40;_QI=48|0|0.5|1|0|0|2|0|91 |
| Chr3 | exon | 30732110 | 30732303 | 30732110 | ID=MsG0380013117.01.T01:exon:6521;Parent=MsG0380013117.01.T01 |
| Chr3 | exon | 30731590 | 30731719 | 30731590 | ID=MsG0380013117.01.T01:exon:6520;Parent=MsG0380013117.01.T01 |
| Chr3 | five_prime_UTR | 30731590 | 30731637 | 30731590 | ID=MsG0380013117.01.T01:five_prime_utr;Parent=MsG0380013117.01.T01 |
| Chr3 | CDS | 30731638 | 30731719 | 30731638 | ID=MsG0380013117.01.T01:cds;Parent=MsG0380013117.01.T01 |
| Chr3 | CDS | 30732110 | 30732303 | 30732110 | ID=MsG0380013117.01.T01:cds;Parent=MsG0380013117.01.T01 |
| Gene Sequence |
| Protein sequence |
