AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013212.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013212.01.T01 MTR_3g436100 85.366 82 12 0 1 82 73 154 4.43e-48 149
MsG0380013212.01.T01 MTR_7g044920 63.855 83 29 1 1 82 70 152 6.24e-35 116
MsG0380013212.01.T01 MTR_7g044980 63.415 82 29 1 2 82 64 145 2.05e-33 112
MsG0380013212.01.T01 MTR_8g056800 54.321 81 37 0 2 82 21 101 5.74e-28 97.1
MsG0380013212.01.T01 MTR_6g017170 71.429 56 15 1 1 55 68 123 1.01e-24 89.7
MsG0380013212.01.T01 MTR_3g067437 66.102 59 20 0 2 60 69 127 2.31e-24 89.0
MsG0380013212.01.T01 MTR_8g022400 67.857 56 17 1 2 56 69 124 1.07e-23 87.8
MsG0380013212.01.T01 MTR_8g022420 62.500 64 23 1 2 64 68 131 3.78e-23 86.3
MsG0380013212.01.T01 MTR_8g022430 59.091 66 25 2 1 64 68 133 6.01e-23 85.5
MsG0380013212.01.T01 MTR_3g067500 56.452 62 27 0 1 62 46 107 3.07e-22 83.2
MsG0380013212.01.T01 MTR_6g017150 55.844 77 22 2 1 76 68 133 5.27e-22 83.2
MsG0380013212.01.T01 MTR_3g067580 55.385 65 28 1 2 65 77 141 5.90e-21 80.9
MsG0380013212.01.T01 MTR_3g467760 52.941 68 31 1 2 68 43 110 6.82e-21 79.7
MsG0380013212.01.T01 MTR_3g067540 49.351 77 28 1 2 78 73 138 8.25e-21 80.1
MsG0380013212.01.T01 MTR_3g067555 49.351 77 28 1 2 78 73 138 8.25e-21 80.1
MsG0380013212.01.T01 MTR_3g067555 49.351 77 28 1 2 78 60 125 9.62e-21 79.7
MsG0380013212.01.T01 MTR_3g067430 48.052 77 29 1 2 78 73 138 1.16e-20 79.7
MsG0380013212.01.T01 MTR_3g067550 49.351 77 28 1 2 78 52 117 1.17e-20 79.3
MsG0380013212.01.T01 MTR_3g067555 49.351 77 28 1 2 78 52 117 1.17e-20 79.3
MsG0380013212.01.T01 MTR_7g056803 57.377 61 25 1 5 64 74 134 1.28e-20 79.7
MsG0380013212.01.T01 MTR_3g067270 52.941 68 31 1 2 68 67 134 1.71e-20 79.3
MsG0380013212.01.T01 MTR_3g467750 52.941 68 31 1 2 68 67 134 1.71e-20 79.3
MsG0380013212.01.T01 MTR_7g056817 53.846 65 29 1 1 64 71 135 1.92e-20 79.3
MsG0380013212.01.T01 MTR_3g067280 52.941 68 31 1 2 68 67 134 2.08e-20 79.0
MsG0380013212.01.T01 MTR_3g463570 47.500 80 30 2 2 80 68 136 3.07e-20 78.6
MsG0380013212.01.T01 MTR_3g067570 67.391 46 15 0 2 47 45 90 1.01e-19 76.3
MsG0380013212.01.T01 MTR_3g463570 47.500 80 30 2 2 80 88 156 1.08e-19 77.8
MsG0380013212.01.T01 MTR_3g067535 53.247 77 23 2 2 78 71 134 1.18e-19 77.0
MsG0380013212.01.T01 MTR_4g026590 47.561 82 32 1 1 82 75 145 2.44e-19 76.6
MsG0380013212.01.T01 MTR_6g036620 47.436 78 30 1 1 78 66 132 6.38e-19 75.1
MsG0380013212.01.T01 MTR_6g047900 46.341 82 31 3 1 81 65 134 8.10e-19 75.1
MsG0380013212.01.T01 MTR_3g067510 67.391 46 15 0 2 47 75 120 1.02e-18 74.7
MsG0380013212.01.T01 MTR_3g067510 67.391 46 15 0 2 47 70 115 1.12e-18 74.3
MsG0380013212.01.T01 MTR_8g461020 47.945 73 27 2 1 72 67 129 5.74e-18 72.8
MsG0380013212.01.T01 MTR_6g047880 44.444 81 33 2 1 81 69 137 1.49e-17 71.6
MsG0380013212.01.T01 MTR_3g436120 82.500 40 7 0 43 82 62 101 1.43e-15 65.9
MsG0380013212.01.T01 MTR_1g019650 50.980 51 25 0 5 55 60 110 1.86e-15 65.9
MsG0380013212.01.T01 MTR_3g067445 55.556 45 20 0 2 46 73 117 2.06e-15 65.9
MsG0380013212.01.T01 MTR_7g029540 40.385 52 31 0 1 52 69 120 8.56e-12 56.6
MsG0380013212.01.T01 MTR_6g082060 62.500 40 15 0 19 58 91 130 2.31e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATGCTGATATTGAATA+TGG 0.285356 3:+32979093 MsG0380013212.01.T01:CDS
AGACTACTTGAAGATGGCTT+TGG 0.386657 3:+32979218 MsG0380013212.01.T01:CDS
GCAGAACGTTACTTCAAGAT+TGG 0.399752 3:+32979141 MsG0380013212.01.T01:CDS
CGAGACATATCATTCTTAAG+TGG 0.433054 3:-32979247 None:intergenic
CCATATTCAATATCAGCATT+AGG 0.437256 3:-32979093 None:intergenic
GAGTGCATCAAGAAGGGAAG+TGG 0.514496 3:+32979051 MsG0380013212.01.T01:CDS
CGTTCTGCTTCATTTACAGA+GGG 0.566815 3:-32979126 None:intergenic
TCATGCGGAGTGCATCAAGA+AGG 0.571938 3:+32979044 MsG0380013212.01.T01:CDS
CATGCGGAGTGCATCAAGAA+GGG 0.592268 3:+32979045 MsG0380013212.01.T01:CDS
ACGTTCTGCTTCATTTACAG+AGG 0.626101 3:-32979127 None:intergenic
AGTGCATCAAGAAGGGAAGT+GGG 0.636415 3:+32979052 MsG0380013212.01.T01:CDS
ATGCTGATATTGAATATGGA+TGG 0.639133 3:+32979097 MsG0380013212.01.T01:CDS
TAACTTATGCAAAACTCATG+CGG 0.658689 3:+32979029 MsG0380013212.01.T01:CDS
ACATTATATGAAGATGATCG+AGG 0.681516 3:+32978999 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAGAAATTTTTTACTATA+GGG - Chr3:32979173-32979192 None:intergenic 15.0%
!!! TTGAAGAAATTTTTTACTAT+AGG - Chr3:32979174-32979193 None:intergenic 15.0%
!!! GAAATTTTTTACTATAGGGT+TGG - Chr3:32979169-32979188 None:intergenic 25.0%
ATGCTGATATTGAATATGGA+TGG + Chr3:32979097-32979116 MsG0380013212.01.T01:CDS 30.0%
CCATATTCAATATCAGCATT+AGG - Chr3:32979096-32979115 None:intergenic 30.0%
CCTAATGCTGATATTGAATA+TGG + Chr3:32979093-32979112 MsG0380013212.01.T01:CDS 30.0%
TAACTTATGCAAAACTCATG+CGG + Chr3:32979029-32979048 MsG0380013212.01.T01:CDS 30.0%
TGAAAAAGACTACTTGAAGA+TGG + Chr3:32979212-32979231 MsG0380013212.01.T01:CDS 30.0%
!! GTCTTTTTCATTAGAGTTTG+AGG - Chr3:32979202-32979221 None:intergenic 30.0%
! GCATGAGTTTTGCATAAGTT+AGG - Chr3:32979030-32979049 None:intergenic 35.0%
ACGTTCTGCTTCATTTACAG+AGG - Chr3:32979130-32979149 None:intergenic 40.0%
AGACTACTTGAAGATGGCTT+TGG + Chr3:32979218-32979237 MsG0380013212.01.T01:CDS 40.0%
CGTTCTGCTTCATTTACAGA+GGG - Chr3:32979129-32979148 None:intergenic 40.0%
GCAGAACGTTACTTCAAGAT+TGG + Chr3:32979141-32979160 MsG0380013212.01.T01:CDS 40.0%
AGTGCATCAAGAAGGGAAGT+GGG + Chr3:32979052-32979071 MsG0380013212.01.T01:CDS 45.0%
CATGCGGAGTGCATCAAGAA+GGG + Chr3:32979045-32979064 MsG0380013212.01.T01:CDS 50.0%
GAGTGCATCAAGAAGGGAAG+TGG + Chr3:32979051-32979070 MsG0380013212.01.T01:CDS 50.0%
TCATGCGGAGTGCATCAAGA+AGG + Chr3:32979044-32979063 MsG0380013212.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 32979006 32979254 32979006 ID=MsG0380013212.01;Name=MsG0380013212.01
Chr3 mRNA 32979006 32979254 32979006 ID=MsG0380013212.01.T01;Parent=MsG0380013212.01;Name=MsG0380013212.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|82
Chr3 exon 32979006 32979254 32979006 ID=MsG0380013212.01.T01:exon:6273;Parent=MsG0380013212.01.T01
Chr3 CDS 32979006 32979254 32979006 ID=MsG0380013212.01.T01:cds;Parent=MsG0380013212.01.T01
Gene Sequence

>MsG0380013212.01.T01

ATGAAGATGATCGAGGAACATCCTAACTTATGCAAAACTCATGCGGAGTGCATCAAGAAGGGAAGTGGGAACTTTTGTGCTCGTTATCCTAATGCTGATATTGAATATGGATGGTGTTTTCCCTCTGTAAATGAAGCAGAACGTTACTTCAAGATTGGATCCAACCCTATAGTAAAAAATTTCTTCAAGATTGCCTCAAACTCTAATGAAAAAGACTACTTGAAGATGGCTTTGGAAATTGCCACTTAA

Protein sequence

>MsG0380013212.01.T01

MKMIEEHPNLCKTHAECIKKGSGNFCARYPNADIEYGWCFPSVNEAERYFKIGSNPIVKNFFKIASNSNEKDYLKMALEIAT*