Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005059.01.T01 | XP_013469340.2 | 69.091 | 275 | 81 | 2 | 1 | 272 | 1 | 274 | 2.83E-127 | 373 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005059.01.T01 | Q41160 | 53.818 | 275 | 118 | 4 | 5 | 273 | 1 | 272 | 5.44E-95 | 283 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005059.01.T01 | A0A072VPS3 | 69.091 | 275 | 81 | 2 | 1 | 272 | 1 | 274 | 8.14e-127 | 371 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048509.01 | MsG0180005059.01 | 0.811141 | 8.122100e-51 | 4.988348e-48 |
| MsG0180003320.01 | MsG0180005059.01 | 0.804604 | 1.994688e-49 | 1.032876e-46 |
| MsG0180003375.01 | MsG0180005059.01 | 0.805399 | 1.360531e-49 | 7.190551e-47 |
| MsG0180003600.01 | MsG0180005059.01 | 0.816218 | 6.192548e-52 | 4.361812e-49 |
| MsG0180004424.01 | MsG0180005059.01 | 0.819954 | 8.855702e-53 | 6.913664e-50 |
| MsG0180005059.01 | MsG0180005060.01 | 0.887726 | 1.107498e-72 | 8.333326e-69 |
| MsG0180005059.01 | MsG0180005080.01 | 0.948194 | 1.527992e-106 | 2.810527e-101 |
| MsG0180005059.01 | MsG0180005082.01 | 0.894393 | 2.571481e-75 | 2.551430e-71 |
| MsG0180005059.01 | MsG0180005130.01 | 0.849484 | 3.140071e-60 | 5.961419e-57 |
| MsG0180005059.01 | MsG0180005143.01 | 0.847432 | 1.160459e-59 | 2.062347e-56 |
| MsG0180005059.01 | MsG0180006253.01 | 0.826924 | 2.076534e-54 | 1.974973e-51 |
| MsG0180005059.01 | MsG0280007513.01 | 0.811232 | 7.759097e-51 | 4.776934e-48 |
| MsG0180005059.01 | MsG0280007677.01 | 0.818960 | 1.491815e-52 | 1.133030e-49 |
| MsG0180005059.01 | MsG0280007689.01 | 0.842471 | 2.533546e-58 | 3.845796e-55 |
| MsG0180005059.01 | MsG0280008178.01 | 0.831078 | 2.046108e-55 | 2.196873e-52 |
| MsG0180005059.01 | MsG0280008180.01 | 0.829189 | 5.915391e-55 | 6.010788e-52 |
| MsG0180005059.01 | MsG0280008226.01 | 0.808072 | 3.706968e-50 | 2.099176e-47 |
| MsG0180005059.01 | MsG0280010425.01 | 0.844400 | 7.740739e-59 | 1.248160e-55 |
| MsG0180005059.01 | MsG0280010623.01 | 0.834660 | 2.636446e-56 | 3.149270e-53 |
| MsG0180005059.01 | MsG0380012222.01 | 0.801392 | 9.201319e-49 | 4.389029e-46 |
| MsG0180005059.01 | MsG0380013212.01 | 0.842519 | 2.460733e-58 | 3.740648e-55 |
| MsG0180005059.01 | MsG0380013213.01 | 0.860129 | 2.562157e-63 | 6.930540e-60 |
| MsG0180005059.01 | MsG0380013214.01 | 0.831165 | 1.947946e-55 | 2.096753e-52 |
| MsG0180005059.01 | MsG0380013226.01 | 0.828038 | 1.122266e-54 | 1.102626e-51 |
| MsG0180005059.01 | MsG0380013239.01 | 0.835641 | 1.490923e-56 | 1.834211e-53 |
| MsG0180005059.01 | MsG0380017200.01 | 0.819234 | 1.292459e-52 | 9.889493e-50 |
| MsG0180005059.01 | MsG0380017901.01 | 0.811497 | 6.796863e-51 | 4.214072e-48 |
| MsG0180005059.01 | MsG0480018439.01 | 0.808440 | 3.094431e-50 | 1.769475e-47 |
| MsG0180005059.01 | MsG0480018559.01 | 0.827169 | 1.814886e-54 | 1.738423e-51 |
| MsG0180005059.01 | MsG0480018617.01 | 0.814136 | 1.796816e-51 | 1.195821e-48 |
| MsG0180005059.01 | MsG0480018692.01 | 0.817634 | 2.979438e-52 | 2.181407e-49 |
| MsG0180005059.01 | MsG0480019322.01 | 0.815286 | 9.990794e-52 | 6.859639e-49 |
| MsG0180005059.01 | MsG0480020904.01 | 0.821535 | 3.835046e-53 | 3.128406e-50 |
| MsG0180005059.01 | MsG0480021091.01 | 0.813849 | 2.078663e-51 | 1.372631e-48 |
| MsG0180005059.01 | MsG0480021133.01 | 0.805936 | 1.049013e-49 | 5.621405e-47 |
| MsG0180005059.01 | MsG0480021234.01 | 0.805255 | 1.458064e-49 | 7.677396e-47 |
| MsG0180005059.01 | MsG0480023432.01 | 0.810473 | 1.132592e-50 | 6.833650e-48 |
| MsG0180005059.01 | MsG0580024994.01 | 0.801994 | 6.925013e-49 | 3.353951e-46 |
| MsG0180005059.01 | MsG0580025007.01 | 0.851180 | 1.049920e-60 | 2.107470e-57 |
| MsG0180005059.01 | MsG0580025024.01 | 0.875994 | 1.986622e-68 | 9.473495e-65 |
| MsG0180005059.01 | MsG0580025193.01 | 0.823655 | 1.232391e-53 | 1.067769e-50 |
| MsG0180005059.01 | MsG0580027776.01 | 0.810857 | 9.358892e-51 | 5.705020e-48 |
| MsG0180005059.01 | MsG0580027777.01 | 0.813039 | 3.132183e-51 | 2.023339e-48 |
| MsG0180005059.01 | MsG0580029937.01 | 0.813310 | 2.731487e-51 | 1.777560e-48 |
| MsG0180005059.01 | MsG0580029938.01 | 0.823233 | 1.546900e-53 | 1.324203e-50 |
| MsG0180005059.01 | MsG0680031200.01 | 0.800913 | 1.153229e-48 | 5.435049e-46 |
| MsG0180005059.01 | MsG0680032026.01 | 0.811822 | 5.775747e-51 | 3.611681e-48 |
| MsG0180005059.01 | MsG0680032461.01 | 0.832336 | 1.002029e-55 | 1.116449e-52 |
| MsG0180005059.01 | MsG0680032932.01 | 0.812108 | 5.003371e-51 | 3.153001e-48 |
| MsG0180005059.01 | MsG0680034238.01 | 0.816229 | 6.158670e-52 | 4.339105e-49 |
| MsG0180005059.01 | MsG0680034254.01 | 0.800839 | 1.194047e-48 | 5.616735e-46 |
| MsG0180005059.01 | MsG0680034354.01 | 0.808057 | 3.733422e-50 | 2.113310e-47 |
| MsG0180005059.01 | MsG0680034391.01 | 0.806573 | 7.704786e-50 | 4.196343e-47 |
| MsG0180005059.01 | MsG0680034974.01 | 0.815099 | 1.099405e-51 | 7.509377e-49 |
| MsG0180005059.01 | MsG0680034978.01 | 0.801612 | 8.294796e-49 | 3.978594e-46 |
| MsG0180005059.01 | MsG0780036259.01 | 0.807742 | 4.356783e-50 | 2.445992e-47 |
| MsG0180005059.01 | MsG0780036260.01 | 0.864951 | 8.397783e-65 | 2.685029e-61 |
| MsG0180005059.01 | MsG0780036376.01 | 0.838807 | 2.309028e-57 | 3.127621e-54 |
| MsG0180005059.01 | MsG0780036835.01 | 0.804678 | 1.924754e-49 | 9.985833e-47 |
| MsG0180005059.01 | MsG0780038307.01 | 0.802029 | 6.809769e-49 | 3.301156e-46 |
| MsG0180005059.01 | MsG0780038318.01 | 0.807388 | 5.178749e-50 | 2.880803e-47 |
| MsG0180005059.01 | MsG0780038519.01 | 0.800195 | 1.614937e-48 | 7.473212e-46 |
| MsG0180005059.01 | MsG0780039686.01 | 0.829897 | 3.980250e-55 | 4.128889e-52 |
| MsG0180005059.01 | MsG0780039912.01 | 0.801458 | 8.920898e-49 | 4.262353e-46 |
| MsG0180005059.01 | MsG0780039923.01 | 0.837033 | 6.598264e-57 | 8.465690e-54 |
| MsG0180005059.01 | MsG0780040670.01 | 0.808134 | 3.595229e-50 | 2.039293e-47 |
| MsG0180005059.01 | MsG0780040684.01 | -0.803069 | 4.156136e-49 | 2.068750e-46 |
| MsG0180005059.01 | MsG0780040716.01 | 0.801240 | 9.883549e-49 | 4.696693e-46 |
| MsG0180005059.01 | MsG0780041447.01 | 0.804552 | 2.044949e-49 | 1.057468e-46 |
| MsG0180005059.01 | MsG0880042633.01 | 0.822371 | 2.455688e-53 | 2.051643e-50 |
| MsG0180005059.01 | MsG0880042642.01 | 0.846460 | 2.141641e-59 | 3.689315e-56 |
| MsG0180005059.01 | MsG0880045801.01 | 0.803102 | 4.091504e-49 | 2.038307e-46 |
| MsG0180005059.01 | MsG0880045854.01 | 0.830374 | 3.045262e-55 | 3.202514e-52 |
| MsG0180005059.01 | MsG0880046166.01 | 0.805395 | 1.363059e-49 | 7.203140e-47 |
| MsG0180005059.01 | MsG0880046870.01 | 0.800506 | 1.396033e-48 | 6.511392e-46 |
| MsG0180005059.01 | MsG0880046946.01 | 0.836517 | 8.936011e-57 | 1.128719e-53 |
| MsG0180005059.01 | MsG0880047259.01 | 0.817475 | 3.235352e-52 | 2.358660e-49 |
| MsG0180005059.01 | MsG0880047312.01 | 0.803509 | 3.370611e-49 | 1.696953e-46 |
| MsG0180005059.01 | MsG0880047450.01 | 0.806568 | 7.720601e-50 | 4.204505e-47 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005059.01.T01 | MTR_1g090973 | 69.091 | 275 | 81 | 2 | 1 | 272 | 1 | 274 | 2.07e-130 | 371 |
| MsG0180005059.01.T01 | MTR_1g090960 | 61.566 | 281 | 62 | 4 | 1 | 272 | 1 | 244 | 2.16e-108 | 314 |
| MsG0180005059.01.T01 | MTR_3g054170 | 56.432 | 241 | 102 | 2 | 21 | 261 | 17 | 254 | 9.99e-92 | 272 |
| MsG0180005059.01.T01 | MTR_0163s0020 | 50.357 | 280 | 130 | 3 | 1 | 273 | 2 | 279 | 8.77e-90 | 268 |
| MsG0180005059.01.T01 | MTR_0684s0020 | 50.357 | 280 | 130 | 3 | 1 | 273 | 1 | 278 | 8.87e-90 | 268 |
| MsG0180005059.01.T01 | MTR_3g047140 | 52.593 | 270 | 126 | 2 | 1 | 269 | 1 | 269 | 5.47e-89 | 266 |
| MsG0180005059.01.T01 | MTR_3g054200 | 53.942 | 241 | 108 | 2 | 21 | 261 | 17 | 254 | 8.06e-87 | 259 |
| MsG0180005059.01.T01 | MTR_3g054180 | 53.086 | 243 | 109 | 3 | 21 | 261 | 21 | 260 | 1.30e-83 | 251 |
| MsG0180005059.01.T01 | MTR_1g090943 | 49.603 | 252 | 123 | 3 | 14 | 264 | 15 | 263 | 1.00e-70 | 219 |
| MsG0180005059.01.T01 | MTR_3g027280 | 47.826 | 276 | 126 | 11 | 1 | 264 | 1 | 270 | 2.14e-66 | 208 |
| MsG0180005059.01.T01 | MTR_1g090957 | 44.056 | 286 | 132 | 11 | 1 | 275 | 1 | 269 | 7.55e-59 | 189 |
| MsG0180005059.01.T01 | MTR_1g090937 | 43.066 | 274 | 137 | 10 | 1 | 265 | 1 | 264 | 1.81e-57 | 185 |
| MsG0180005059.01.T01 | MTR_1g090930 | 41.877 | 277 | 140 | 10 | 1 | 265 | 1 | 268 | 5.37e-52 | 171 |
| MsG0180005059.01.T01 | MTR_8g068040 | 40.755 | 265 | 129 | 11 | 14 | 262 | 10 | 262 | 1.10e-47 | 160 |
| MsG0180005059.01.T01 | MTR_8g068030 | 39.630 | 270 | 129 | 12 | 14 | 262 | 10 | 266 | 2.37e-46 | 156 |
| MsG0180005059.01.T01 | MTR_5g031160 | 38.194 | 288 | 138 | 13 | 6 | 261 | 8 | 287 | 2.95e-44 | 151 |
| MsG0180005059.01.T01 | MTR_5g031120 | 40.562 | 249 | 119 | 11 | 35 | 261 | 38 | 279 | 1.38e-42 | 147 |
| MsG0180005059.01.T01 | MTR_5g031100 | 39.919 | 248 | 121 | 10 | 35 | 261 | 38 | 278 | 2.71e-42 | 146 |
| MsG0180005059.01.T01 | MTR_5g031140 | 36.585 | 287 | 144 | 13 | 5 | 261 | 8 | 286 | 5.65e-41 | 143 |
| MsG0180005059.01.T01 | MTR_4g056320 | 36.567 | 268 | 126 | 7 | 2 | 265 | 6 | 233 | 1.38e-40 | 141 |
| MsG0180005059.01.T01 | MTR_8g067980 | 39.662 | 237 | 126 | 8 | 36 | 259 | 25 | 257 | 1.60e-40 | 141 |
| MsG0180005059.01.T01 | MTR_8g023510 | 38.806 | 268 | 148 | 10 | 1 | 261 | 1 | 259 | 3.33e-39 | 138 |
| MsG0180005059.01.T01 | MTR_8g067990 | 37.553 | 237 | 131 | 8 | 36 | 259 | 25 | 257 | 1.54e-38 | 136 |
| MsG0180005059.01.T01 | MTR_8g068050 | 36.765 | 272 | 151 | 10 | 11 | 269 | 18 | 281 | 3.40e-38 | 142 |
| MsG0180005059.01.T01 | MTR_4g023810 | 38.983 | 236 | 132 | 8 | 30 | 261 | 27 | 254 | 1.81e-34 | 125 |
| MsG0180005059.01.T01 | MTR_1g014240 | 37.860 | 243 | 131 | 9 | 38 | 264 | 40 | 278 | 9.50e-33 | 127 |
| MsG0180005059.01.T01 | MTR_4g093080 | 35.926 | 270 | 143 | 12 | 17 | 268 | 9 | 266 | 9.82e-33 | 127 |
| MsG0180005059.01.T01 | MTR_8g067960 | 39.732 | 224 | 119 | 9 | 49 | 261 | 23 | 241 | 5.95e-32 | 118 |
| MsG0180005059.01.T01 | MTR_4g056360 | 34.211 | 266 | 131 | 10 | 2 | 261 | 6 | 233 | 9.53e-31 | 115 |
| MsG0180005059.01.T01 | MTR_5g031090 | 34.553 | 246 | 135 | 11 | 37 | 261 | 35 | 275 | 1.35e-29 | 113 |
| MsG0180005059.01.T01 | MTR_8g067940 | 35.252 | 278 | 147 | 15 | 8 | 261 | 13 | 281 | 9.20e-29 | 111 |
| MsG0180005059.01.T01 | MTR_5g031030 | 32.922 | 243 | 143 | 8 | 35 | 261 | 36 | 274 | 1.06e-28 | 110 |
| MsG0180005059.01.T01 | MTR_8g067720 | 34.559 | 272 | 155 | 11 | 9 | 268 | 4 | 264 | 1.56e-27 | 111 |
| MsG0180005059.01.T01 | MTR_7g115740 | 32.157 | 255 | 152 | 9 | 23 | 262 | 13 | 261 | 2.10e-27 | 111 |
| MsG0180005059.01.T01 | MTR_7g116130 | 31.461 | 267 | 162 | 7 | 15 | 266 | 6 | 266 | 2.45e-27 | 111 |
| MsG0180005059.01.T01 | MTR_8g067690 | 36.555 | 238 | 137 | 8 | 35 | 261 | 60 | 294 | 1.07e-26 | 109 |
| MsG0180005059.01.T01 | MTR_4g093110 | 34.363 | 259 | 143 | 13 | 19 | 262 | 19 | 265 | 1.19e-25 | 106 |
| MsG0180005059.01.T01 | MTR_8g067735 | 35.391 | 243 | 140 | 9 | 35 | 264 | 43 | 281 | 1.65e-25 | 105 |
| MsG0180005059.01.T01 | MTR_8g067930 | 34.586 | 266 | 155 | 9 | 15 | 268 | 8 | 266 | 1.97e-25 | 105 |
| MsG0180005059.01.T01 | MTR_4g093040 | 33.208 | 265 | 155 | 8 | 5 | 254 | 3 | 260 | 1.09e-24 | 103 |
| MsG0180005059.01.T01 | MTR_4g093140 | 32.819 | 259 | 147 | 12 | 19 | 262 | 19 | 265 | 1.48e-24 | 103 |
| MsG0180005059.01.T01 | MTR_4g093070 | 35.354 | 198 | 114 | 6 | 77 | 262 | 72 | 267 | 1.25e-22 | 97.4 |
| MsG0180005059.01.T01 | MTR_8g067630 | 34.375 | 224 | 133 | 7 | 35 | 247 | 30 | 250 | 2.13e-22 | 97.1 |
| MsG0180005059.01.T01 | MTR_3g463370 | 29.368 | 269 | 136 | 7 | 11 | 269 | 6 | 230 | 3.48e-20 | 87.4 |
| MsG0180005059.01.T01 | MTR_4g093040 | 37.569 | 181 | 95 | 6 | 87 | 254 | 3 | 178 | 5.59e-20 | 89.7 |
| MsG0180005059.01.T01 | MTR_4g093050 | 32.773 | 238 | 142 | 11 | 36 | 257 | 27 | 262 | 2.20e-18 | 85.1 |
| MsG0180005059.01.T01 | MTR_5g068770 | 32.389 | 247 | 135 | 11 | 38 | 259 | 38 | 277 | 3.81e-17 | 81.6 |
| MsG0180005059.01.T01 | MTR_1g048360 | 29.744 | 195 | 117 | 6 | 77 | 259 | 74 | 260 | 5.19e-15 | 75.1 |
| MsG0180005059.01.T01 | MTR_7g115740 | 30.519 | 154 | 98 | 5 | 23 | 170 | 13 | 163 | 1.92e-14 | 73.6 |
| MsG0180005059.01.T01 | MTR_7g062940 | 28.571 | 266 | 149 | 11 | 22 | 260 | 7 | 258 | 3.13e-13 | 69.7 |
| MsG0180005059.01.T01 | MTR_5g025020 | 28.000 | 200 | 119 | 7 | 76 | 255 | 87 | 281 | 9.05e-13 | 68.6 |
| MsG0180005059.01.T01 | MTR_5g077100 | 31.797 | 217 | 125 | 8 | 59 | 263 | 49 | 254 | 1.29e-12 | 68.2 |
| MsG0180005059.01.T01 | MTR_7g062890 | 28.044 | 271 | 167 | 10 | 16 | 265 | 1 | 264 | 2.80e-11 | 63.9 |
| MsG0180005059.01.T01 | MTR_2g090250 | 29.688 | 256 | 150 | 11 | 41 | 272 | 29 | 278 | 3.09e-11 | 63.9 |
| MsG0180005059.01.T01 | MTR_7g063010 | 31.646 | 237 | 123 | 11 | 50 | 260 | 36 | 259 | 9.72e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005059.01.T01 | AT5G10530 | 39.837 | 246 | 121 | 13 | 35 | 262 | 19 | 255 | 2.09e-31 | 123 |
| MsG0180005059.01.T01 | AT5G65600 | 34.081 | 223 | 128 | 9 | 35 | 241 | 35 | 254 | 8.28e-23 | 98.2 |
| MsG0180005059.01.T01 | AT5G06740 | 31.559 | 263 | 138 | 12 | 22 | 258 | 11 | 257 | 7.16e-18 | 83.6 |
| MsG0180005059.01.T01 | AT5G06740 | 31.559 | 263 | 138 | 12 | 22 | 258 | 23 | 269 | 7.21e-18 | 83.6 |
| MsG0180005059.01.T01 | AT5G55830 | 30.224 | 268 | 159 | 11 | 14 | 260 | 7 | 267 | 9.47e-16 | 77.4 |
| MsG0180005059.01.T01 | AT5G03140 | 29.762 | 252 | 159 | 9 | 17 | 255 | 11 | 257 | 1.78e-14 | 73.6 |
| MsG0180005059.01.T01 | AT4G02410 | 27.757 | 263 | 151 | 11 | 31 | 266 | 22 | 272 | 2.94e-13 | 70.1 |
| MsG0180005059.01.T01 | AT3G53810 | 28.287 | 251 | 157 | 7 | 29 | 260 | 18 | 264 | 8.08e-13 | 68.6 |
| MsG0180005059.01.T01 | AT4G04960 | 30.172 | 232 | 130 | 8 | 36 | 244 | 21 | 243 | 8.54e-13 | 68.6 |
| MsG0180005059.01.T01 | AT4G04960 | 30.172 | 232 | 130 | 9 | 36 | 244 | 21 | 243 | 1.17e-12 | 68.2 |
| MsG0180005059.01.T01 | AT4G02420 | 27.820 | 266 | 162 | 11 | 17 | 260 | 6 | 263 | 4.97e-12 | 66.2 |
| MsG0180005059.01.T01 | AT5G01560 | 30.000 | 250 | 142 | 10 | 38 | 259 | 30 | 274 | 7.48e-12 | 65.9 |
| MsG0180005059.01.T01 | AT2G37710 | 26.000 | 250 | 160 | 8 | 31 | 260 | 20 | 264 | 8.68e-12 | 65.5 |
| MsG0180005059.01.T01 | AT4G29050 | 27.753 | 227 | 128 | 9 | 60 | 260 | 59 | 275 | 1.23e-11 | 65.1 |
| MsG0180005059.01.T01 | AT4G29050 | 27.753 | 227 | 128 | 9 | 60 | 260 | 45 | 261 | 1.36e-11 | 65.1 |
Find 75 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCCAACATGTGTGTATT+TGG | 0.093315 | 1:+86342064 | None:intergenic |
| TTTCTTTAAAGAAAATAAAA+TGG | 0.247146 | 1:-86342025 | MsG0180005059.01.T01:CDS |
| ACTTCCTTGTAGGGAAATTA+TGG | 0.265257 | 1:+86342415 | None:intergenic |
| ATTCAGCACCGGTGCCCATT+TGG | 0.272619 | 1:-86342316 | MsG0180005059.01.T01:CDS |
| GAAAAGCTTGCAATATTGTT+TGG | 0.279510 | 1:+86341762 | None:intergenic |
| CCCAACAGATCCAAACTATT+TGG | 0.303319 | 1:-86342354 | MsG0180005059.01.T01:CDS |
| TTCAGCACCGGTGCCCATTT+GGG | 0.316239 | 1:-86342315 | MsG0180005059.01.T01:CDS |
| TAAAGAAAATAAAATGGAAT+AGG | 0.318424 | 1:-86342019 | MsG0180005059.01.T01:CDS |
| AGTTGAGTTGCTAGGAATAT+TGG | 0.321296 | 1:+86342175 | None:intergenic |
| TGACTAACACAACAGAAAAT+AGG | 0.328825 | 1:+86342531 | None:intergenic |
| ATTCCAACATGTGTGTATTT+GGG | 0.336887 | 1:+86342065 | None:intergenic |
| AAAAGCTTGCAATATTGTTT+GGG | 0.344232 | 1:+86341763 | None:intergenic |
| CATCAGATGTGACAAACTTT+TGG | 0.346785 | 1:+86342231 | None:intergenic |
| GCCCGACCAGCCAAATAGTT+TGG | 0.378898 | 1:+86342344 | None:intergenic |
| GCCAAATAGTTTGGATCTGT+TGG | 0.383215 | 1:+86342353 | None:intergenic |
| GCCAGTGGTTTCATCCCAAA+TGG | 0.385140 | 1:+86342301 | None:intergenic |
| AAATTGAGGCACTTCCTTGT+AGG | 0.404967 | 1:+86342405 | None:intergenic |
| TGGGCACCGGTGCTGAATAC+AGG | 0.408005 | 1:+86342321 | None:intergenic |
| GGATTTACTATTACTGAAGT+TGG | 0.409756 | 1:+86342436 | None:intergenic |
| AAAGAAAATAAAATGGAATA+GGG | 0.416250 | 1:-86342018 | MsG0180005059.01.T01:CDS |
| AGCTAAACAACCTCCATTCA+TGG | 0.418791 | 1:-86341815 | MsG0180005059.01.T01:CDS |
| TTGACTTGAAAGACCATGAA+TGG | 0.422733 | 1:+86341802 | None:intergenic |
| CAAAAGTTTGTCACATCTGA+TGG | 0.426781 | 1:-86342230 | MsG0180005059.01.T01:CDS |
| AATTGAGGCACTTCCTTGTA+GGG | 0.429899 | 1:+86342406 | None:intergenic |
| AAACCAATCCTTACGGTGTT+CGG | 0.435951 | 1:+86341867 | None:intergenic |
| ATAGTTGAAAAGGTACCATC+TGG | 0.449708 | 1:+86341921 | None:intergenic |
| TCCGCATACAATCCATTTGT+TGG | 0.449749 | 1:-86342122 | MsG0180005059.01.T01:CDS |
| GTATTTGGGATCCCACCTAT+TGG | 0.452286 | 1:+86342079 | None:intergenic |
| CCAAATAGTTTGGATCTGTT+GGG | 0.452907 | 1:+86342354 | None:intergenic |
| TCCAAACTATTTGGCTGGTC+GGG | 0.455927 | 1:-86342345 | MsG0180005059.01.T01:CDS |
| ATGGTCTTTCAAGTCAAACT+TGG | 0.459323 | 1:-86341796 | MsG0180005059.01.T01:CDS |
| AGTTTGATAGTTATGCCAAT+AGG | 0.461031 | 1:-86342094 | MsG0180005059.01.T01:CDS |
| CTTCCGAACACCGTAAGGAT+TGG | 0.469222 | 1:-86341870 | MsG0180005059.01.T01:CDS |
| TCAAACTCTACACCAACAAA+TGG | 0.477349 | 1:+86342110 | None:intergenic |
| GACTTGAGCAATAGTTGAAA+AGG | 0.492838 | 1:+86341911 | None:intergenic |
| TGTCTTAGAGAGATTGTCAT+AGG | 0.497716 | 1:+86341965 | None:intergenic |
| ATAAAATGGAATAGGGTGAA+TGG | 0.502888 | 1:-86342011 | MsG0180005059.01.T01:CDS |
| AACTCAACTGGTGGAAACCT+TGG | 0.508020 | 1:-86342161 | MsG0180005059.01.T01:CDS |
| GGCTATATCCAAGATTCCAT+TGG | 0.512019 | 1:+86342379 | None:intergenic |
| GATTCCATTGGATAAAATTG+AGG | 0.512081 | 1:+86342391 | None:intergenic |
| ATCCAAACTATTTGGCTGGT+CGG | 0.513232 | 1:-86342346 | MsG0180005059.01.T01:CDS |
| CCAGTGGTTTCATCCCAAAT+GGG | 0.521697 | 1:+86342302 | None:intergenic |
| TTTCCACCAGTTGAGTTGCT+AGG | 0.525423 | 1:+86342167 | None:intergenic |
| ATGGAGGTTGTTTAGCTGAC+CGG | 0.532538 | 1:+86341821 | None:intergenic |
| AATATTCCTAGCAACTCAAC+TGG | 0.536663 | 1:-86342173 | MsG0180005059.01.T01:CDS |
| GGAATATTGGCTCGATCTTG+TGG | 0.539230 | 1:+86342188 | None:intergenic |
| TCGGGCCCTGTATTCAGCAC+CGG | 0.539297 | 1:-86342327 | MsG0180005059.01.T01:CDS |
| ACAGATCCAAACTATTTGGC+TGG | 0.541475 | 1:-86342350 | MsG0180005059.01.T01:CDS |
| GGGCACCGGTGCTGAATACA+GGG | 0.542627 | 1:+86342322 | None:intergenic |
| TTGATAGTTATGCCAATAGG+TGG | 0.543485 | 1:-86342091 | MsG0180005059.01.T01:CDS |
| CCCATTTGGGATGAAACCAC+TGG | 0.547396 | 1:-86342302 | MsG0180005059.01.T01:CDS |
| ATAGTTTGGATCTGTTGGGT+TGG | 0.547533 | 1:+86342358 | None:intergenic |
| GAATAGGGTGAATGGCGCCT+TGG | 0.550930 | 1:-86342003 | MsG0180005059.01.T01:CDS |
| CAGAGCTTCCGAACACCGTA+AGG | 0.561999 | 1:-86341875 | MsG0180005059.01.T01:CDS |
| GCTGCCACATCAAGAGGTGC+CGG | 0.566269 | 1:-86341840 | MsG0180005059.01.T01:CDS |
| ATCGATGAAACCAAATCAGA+TGG | 0.568016 | 1:+86341737 | None:intergenic |
| GTCATGCACTCTGAATGAAA+AGG | 0.571753 | 1:+86342256 | None:intergenic |
| GATCCCAAATACACACATGT+TGG | 0.576936 | 1:-86342068 | MsG0180005059.01.T01:CDS |
| AAATCCATAATTTCCCTACA+AGG | 0.579177 | 1:-86342419 | MsG0180005059.01.T01:CDS |
| ATTCCTAGCAACTCAACTGG+TGG | 0.582588 | 1:-86342170 | MsG0180005059.01.T01:CDS |
| ACTGAAGTTGGTGATGTCGA+AGG | 0.592142 | 1:+86342448 | None:intergenic |
| GAAGCTCGCCACGTTGCCAG+TGG | 0.595816 | 1:+86342286 | None:intergenic |
| GTTTCATCCCAAATGGGCAC+CGG | 0.596050 | 1:+86342308 | None:intergenic |
| TGATAGTTATGCCAATAGGT+GGG | 0.619156 | 1:-86342090 | MsG0180005059.01.T01:CDS |
| ATTTGTTGGAAAGAAAGCCA+TGG | 0.619705 | 1:+86342559 | None:intergenic |
| GTGGTTGTATCTTACCCAGA+TGG | 0.625936 | 1:-86341936 | MsG0180005059.01.T01:CDS |
| CTGACCGGCACCTCTTGATG+TGG | 0.630323 | 1:+86341836 | None:intergenic |
| GCTTGCAATATTGTTTGGGG+TGG | 0.636102 | 1:+86341767 | None:intergenic |
| AAAGCTTGCAATATTGTTTG+GGG | 0.645833 | 1:+86341764 | None:intergenic |
| TAGTTGAAAAGGTACCATCT+GGG | 0.650948 | 1:+86341922 | None:intergenic |
| TCTCTCTAAGACATTGAATG+TGG | 0.660926 | 1:-86341955 | MsG0180005059.01.T01:CDS |
| ACCAACAAATGGATTGTATG+CGG | 0.667112 | 1:+86342121 | None:intergenic |
| ACTTGAAAGACCATGAATGG+AGG | 0.674593 | 1:+86341805 | None:intergenic |
| GGAGATATTAACATCGACCA+AGG | 0.682054 | 1:+86341986 | None:intergenic |
| GGATGAAACCACTGGCAACG+TGG | 0.707919 | 1:-86342294 | MsG0180005059.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCTTTAAAGAAAATAAAA+TGG | - | Chr1:86342284-86342303 | MsG0180005059.01.T01:CDS | 10.0% |
| !! | AAAGAAAATAAAATGGAATA+GGG | - | Chr1:86342291-86342310 | MsG0180005059.01.T01:CDS | 15.0% |
| !! | TAAAGAAAATAAAATGGAAT+AGG | - | Chr1:86342290-86342309 | MsG0180005059.01.T01:CDS | 15.0% |
| ! | AAAAGCTTGCAATATTGTTT+GGG | + | Chr1:86342549-86342568 | None:intergenic | 25.0% |
| !! | AATTTTATCCAATGGAATCT+TGG | - | Chr1:86341922-86341941 | MsG0180005059.01.T01:CDS | 25.0% |
| !! | AGTTTTCATTAACAATTCCA+AGG | + | Chr1:86342168-86342187 | None:intergenic | 25.0% |
| !!! | GAAAATAGGTTTTGATTTGT+TGG | + | Chr1:86341767-86341786 | None:intergenic | 25.0% |
| AAAGCTTGCAATATTGTTTG+GGG | + | Chr1:86342548-86342567 | None:intergenic | 30.0% | |
| AAATCCATAATTTCCCTACA+AGG | - | Chr1:86341890-86341909 | MsG0180005059.01.T01:CDS | 30.0% | |
| AATTCCAACATGTGTGTATT+TGG | + | Chr1:86342248-86342267 | None:intergenic | 30.0% | |
| ATAAAATGGAATAGGGTGAA+TGG | - | Chr1:86342298-86342317 | MsG0180005059.01.T01:CDS | 30.0% | |
| ATTCCAACATGTGTGTATTT+GGG | + | Chr1:86342247-86342266 | None:intergenic | 30.0% | |
| GAAAAGCTTGCAATATTGTT+TGG | + | Chr1:86342550-86342569 | None:intergenic | 30.0% | |
| GATTCCATTGGATAAAATTG+AGG | + | Chr1:86341921-86341940 | None:intergenic | 30.0% | |
| GGATTTACTATTACTGAAGT+TGG | + | Chr1:86341876-86341895 | None:intergenic | 30.0% | |
| TGACTAACACAACAGAAAAT+AGG | + | Chr1:86341781-86341800 | None:intergenic | 30.0% | |
| ! | AGTTTGATAGTTATGCCAAT+AGG | - | Chr1:86342215-86342234 | MsG0180005059.01.T01:CDS | 30.0% |
| AATATTCCTAGCAACTCAAC+TGG | - | Chr1:86342136-86342155 | MsG0180005059.01.T01:CDS | 35.0% | |
| ACTTCCTTGTAGGGAAATTA+TGG | + | Chr1:86341897-86341916 | None:intergenic | 35.0% | |
| AGCAGAAAAACCAATCCTTA+CGG | + | Chr1:86342452-86342471 | None:intergenic | 35.0% | |
| CAAAAGTTTGTCACATCTGA+TGG | - | Chr1:86342079-86342098 | MsG0180005059.01.T01:CDS | 35.0% | |
| CCAAATAGTTTGGATCTGTT+GGG | + | Chr1:86341958-86341977 | None:intergenic | 35.0% | |
| GACTTGAGCAATAGTTGAAA+AGG | + | Chr1:86342401-86342420 | None:intergenic | 35.0% | |
| TCAAACTCTACACCAACAAA+TGG | + | Chr1:86342202-86342221 | None:intergenic | 35.0% | |
| TGATAGTTATGCCAATAGGT+GGG | - | Chr1:86342219-86342238 | MsG0180005059.01.T01:CDS | 35.0% | |
| TGTCTTAGAGAGATTGTCAT+AGG | + | Chr1:86342347-86342366 | None:intergenic | 35.0% | |
| ! | AGTGCCTCAATTTTATCCAA+TGG | - | Chr1:86341914-86341933 | MsG0180005059.01.T01:CDS | 35.0% |
| ! | AGTTGAGTTGCTAGGAATAT+TGG | + | Chr1:86342137-86342156 | None:intergenic | 35.0% |
| ! | ATGGTCTTTCAAGTCAAACT+TGG | - | Chr1:86342513-86342532 | MsG0180005059.01.T01:CDS | 35.0% |
| ! | CATCAGATGTGACAAACTTT+TGG | + | Chr1:86342081-86342100 | None:intergenic | 35.0% |
| ! | TTGACTTGAAAGACCATGAA+TGG | + | Chr1:86342510-86342529 | None:intergenic | 35.0% |
| ! | TTGATAGTTATGCCAATAGG+TGG | - | Chr1:86342218-86342237 | MsG0180005059.01.T01:CDS | 35.0% |
| !! | ACCAACAAATGGATTGTATG+CGG | + | Chr1:86342191-86342210 | None:intergenic | 35.0% |
| !! | ATAGTTGAAAAGGTACCATC+TGG | + | Chr1:86342391-86342410 | None:intergenic | 35.0% |
| !! | TAGTTGAAAAGGTACCATCT+GGG | + | Chr1:86342390-86342409 | None:intergenic | 35.0% |
| !! | TCTCTCTAAGACATTGAATG+TGG | - | Chr1:86342354-86342373 | MsG0180005059.01.T01:CDS | 35.0% |
| !!! | GAAGGAAACTGTTTTTTGTG+AGG | + | Chr1:86341846-86341865 | None:intergenic | 35.0% |
| AAACCAATCCTTACGGTGTT+CGG | + | Chr1:86342445-86342464 | None:intergenic | 40.0% | |
| ACAGATCCAAACTATTTGGC+TGG | - | Chr1:86341959-86341978 | MsG0180005059.01.T01:CDS | 40.0% | |
| ACTTGAAAGACCATGAATGG+AGG | + | Chr1:86342507-86342526 | None:intergenic | 40.0% | |
| AGCTAAACAACCTCCATTCA+TGG | - | Chr1:86342494-86342513 | MsG0180005059.01.T01:CDS | 40.0% | |
| ATAGTTTGGATCTGTTGGGT+TGG | + | Chr1:86341954-86341973 | None:intergenic | 40.0% | |
| ATCCAAACTATTTGGCTGGT+CGG | - | Chr1:86341963-86341982 | MsG0180005059.01.T01:CDS | 40.0% | |
| CCCAACAGATCCAAACTATT+TGG | - | Chr1:86341955-86341974 | MsG0180005059.01.T01:CDS | 40.0% | |
| GATCCCAAATACACACATGT+TGG | - | Chr1:86342241-86342260 | MsG0180005059.01.T01:CDS | 40.0% | |
| GCCAAATAGTTTGGATCTGT+TGG | + | Chr1:86341959-86341978 | None:intergenic | 40.0% | |
| GGAGATATTAACATCGACCA+AGG | + | Chr1:86342326-86342345 | None:intergenic | 40.0% | |
| GGCTATATCCAAGATTCCAT+TGG | + | Chr1:86341933-86341952 | None:intergenic | 40.0% | |
| GTCATGCACTCTGAATGAAA+AGG | + | Chr1:86342056-86342075 | None:intergenic | 40.0% | |
| TCCGCATACAATCCATTTGT+TGG | - | Chr1:86342187-86342206 | MsG0180005059.01.T01:CDS | 40.0% | |
| ! | AAATTGAGGCACTTCCTTGT+AGG | + | Chr1:86341907-86341926 | None:intergenic | 40.0% |
| ! | AATTGAGGCACTTCCTTGTA+GGG | + | Chr1:86341906-86341925 | None:intergenic | 40.0% |
| AACTCAACTGGTGGAAACCT+TGG | - | Chr1:86342148-86342167 | MsG0180005059.01.T01:CDS | 45.0% | |
| ATGGAGGTTGTTTAGCTGAC+CGG | + | Chr1:86342491-86342510 | None:intergenic | 45.0% | |
| ATTCCTAGCAACTCAACTGG+TGG | - | Chr1:86342139-86342158 | MsG0180005059.01.T01:CDS | 45.0% | |
| CCAGTGGTTTCATCCCAAAT+GGG | + | Chr1:86342010-86342029 | None:intergenic | 45.0% | |
| GCTTGCAATATTGTTTGGGG+TGG | + | Chr1:86342545-86342564 | None:intergenic | 45.0% | |
| GTGGTTGTATCTTACCCAGA+TGG | - | Chr1:86342373-86342392 | MsG0180005059.01.T01:CDS | 45.0% | |
| ! | GTATTTGGGATCCCACCTAT+TGG | + | Chr1:86342233-86342252 | None:intergenic | 45.0% |
| ! | TCCAAACTATTTGGCTGGTC+GGG | - | Chr1:86341964-86341983 | MsG0180005059.01.T01:CDS | 45.0% |
| ! | TTTCCACCAGTTGAGTTGCT+AGG | + | Chr1:86342145-86342164 | None:intergenic | 45.0% |
| ! | TTTTCTGCTGCCACATCAAG+AGG | - | Chr1:86342463-86342482 | MsG0180005059.01.T01:CDS | 45.0% |
| !! | ACTGAAGTTGGTGATGTCGA+AGG | + | Chr1:86341864-86341883 | None:intergenic | 45.0% |
| !! | GGAATATTGGCTCGATCTTG+TGG | + | Chr1:86342124-86342143 | None:intergenic | 45.0% |
| CTTCCGAACACCGTAAGGAT+TGG | - | Chr1:86342439-86342458 | MsG0180005059.01.T01:CDS | 50.0% | |
| GCCAGTGGTTTCATCCCAAA+TGG | + | Chr1:86342011-86342030 | None:intergenic | 50.0% | |
| GTTTCATCCCAAATGGGCAC+CGG | + | Chr1:86342004-86342023 | None:intergenic | 50.0% | |
| ! | CCCATTTGGGATGAAACCAC+TGG | - | Chr1:86342007-86342026 | MsG0180005059.01.T01:CDS | 50.0% |
| CAGAGCTTCCGAACACCGTA+AGG | - | Chr1:86342434-86342453 | MsG0180005059.01.T01:CDS | 55.0% | |
| GAATAGGGTGAATGGCGCCT+TGG | - | Chr1:86342306-86342325 | MsG0180005059.01.T01:CDS | 55.0% | |
| GCCCGACCAGCCAAATAGTT+TGG | + | Chr1:86341968-86341987 | None:intergenic | 55.0% | |
| GGATGAAACCACTGGCAACG+TGG | - | Chr1:86342015-86342034 | MsG0180005059.01.T01:CDS | 55.0% | |
| !! | ATTCAGCACCGGTGCCCATT+TGG | - | Chr1:86341993-86342012 | MsG0180005059.01.T01:CDS | 55.0% |
| !! | TTCAGCACCGGTGCCCATTT+GGG | - | Chr1:86341994-86342013 | MsG0180005059.01.T01:CDS | 55.0% |
| CTGACCGGCACCTCTTGATG+TGG | + | Chr1:86342476-86342495 | None:intergenic | 60.0% | |
| TCGGGCCCTGTATTCAGCAC+CGG | - | Chr1:86341982-86342001 | MsG0180005059.01.T01:CDS | 60.0% | |
| ! | GCTGCCACATCAAGAGGTGC+CGG | - | Chr1:86342469-86342488 | MsG0180005059.01.T01:CDS | 60.0% |
| !! | GGGCACCGGTGCTGAATACA+GGG | + | Chr1:86341990-86342009 | None:intergenic | 60.0% |
| !! | TGGGCACCGGTGCTGAATAC+AGG | + | Chr1:86341991-86342010 | None:intergenic | 60.0% |
| GAAGCTCGCCACGTTGCCAG+TGG | + | Chr1:86342026-86342045 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 86341752 | 86342579 | 86341752 | ID=MsG0180005059.01;Name=MsG0180005059.01 |
| Chr1 | mRNA | 86341752 | 86342579 | 86341752 | ID=MsG0180005059.01.T01;Parent=MsG0180005059.01;Name=MsG0180005059.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|275 |
| Chr1 | exon | 86341752 | 86342579 | 86341752 | ID=MsG0180005059.01.T01:exon:28438;Parent=MsG0180005059.01.T01 |
| Chr1 | CDS | 86341752 | 86342579 | 86341752 | ID=MsG0180005059.01.T01:cds;Parent=MsG0180005059.01.T01 |
| Chr1 | mRNA | 86342117 | 86342260 | 86342117 | ID=MsG0180005059.01.T02;Parent=MsG0180005059.01;Name=MsG0180005059.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|47 |
| Chr1 | exon | 86342117 | 86342260 | 86342117 | ID=MsG0180005059.01.T02:exon:28439;Parent=MsG0180005059.01.T02 |
| Chr1 | CDS | 86342117 | 86342260 | 86342117 | ID=MsG0180005059.01.T02:cds;Parent=MsG0180005059.01.T02 |
| Chr1 | mRNA | 86341901 | 86341960 | 86341901 | ID=MsG0180005059.01.T03;Parent=MsG0180005059.01;Name=MsG0180005059.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|19 |
| Chr1 | exon | 86341901 | 86341960 | 86341901 | ID=MsG0180005059.01.T03:exon:28440;Parent=MsG0180005059.01.T03 |
| Chr1 | CDS | 86341901 | 86341960 | 86341901 | ID=MsG0180005059.01.T03:cds;Parent=MsG0180005059.01.T03 |
| Gene Sequence |
| Protein sequence |
