AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005060.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005060.01.T01 MTR_1g090957 79.339 242 47 2 1 241 1 240 5.12e-131 371
MsG0180005060.01.T01 MTR_3g027280 75.591 254 47 3 1 241 1 252 1.44e-127 363
MsG0180005060.01.T01 MTR_1g090930 71.825 252 59 3 1 242 1 250 1.81e-118 340
MsG0180005060.01.T01 MTR_1g090937 69.076 249 65 3 1 241 1 245 2.50e-118 339
MsG0180005060.01.T01 MTR_3g054170 45.238 252 106 8 6 241 3 238 5.24e-55 177
MsG0180005060.01.T01 MTR_3g054180 46.185 249 100 8 6 236 7 239 1.61e-52 171
MsG0180005060.01.T01 MTR_0163s0020 44.355 248 120 5 1 238 2 241 3.41e-49 163
MsG0180005060.01.T01 MTR_0684s0020 44.355 248 120 5 1 238 1 240 4.03e-49 163
MsG0180005060.01.T01 MTR_1g090973 44.151 265 117 13 1 249 1 250 4.94e-49 162
MsG0180005060.01.T01 MTR_3g054200 43.548 248 108 7 6 237 3 234 2.58e-48 160
MsG0180005060.01.T01 MTR_1g090943 40.711 253 125 7 1 238 1 243 4.39e-45 152
MsG0180005060.01.T01 MTR_3g047140 40.927 259 115 10 1 238 1 242 1.46e-43 149
MsG0180005060.01.T01 MTR_1g090960 39.331 239 110 5 1 238 1 205 1.40e-38 135
MsG0180005060.01.T01 MTR_8g068050 34.940 249 125 10 14 238 20 255 7.47e-25 103
MsG0180005060.01.T01 MTR_3g463370 32.751 229 110 5 18 238 12 204 5.48e-24 96.7
MsG0180005060.01.T01 MTR_8g023510 34.483 261 128 11 1 238 1 241 1.10e-23 96.7
MsG0180005060.01.T01 MTR_5g031120 32.824 262 116 13 23 238 14 261 1.87e-21 90.9
MsG0180005060.01.T01 MTR_8g068040 33.898 236 126 10 23 238 18 243 1.43e-20 88.2
MsG0180005060.01.T01 MTR_8g067980 35.266 207 106 10 54 238 41 241 1.98e-20 87.8
MsG0180005060.01.T01 MTR_8g068030 32.787 244 122 14 23 238 18 247 4.31e-19 84.3
MsG0180005060.01.T01 MTR_5g031100 31.373 255 128 12 23 238 14 260 1.84e-18 82.8
MsG0180005060.01.T01 MTR_8g067990 31.373 204 118 9 54 238 41 241 5.42e-18 81.3
MsG0180005060.01.T01 MTR_8g067940 31.008 258 132 13 15 238 18 263 9.15e-18 80.9
MsG0180005060.01.T01 MTR_4g023810 32.301 226 120 11 30 238 27 236 1.02e-17 80.5
MsG0180005060.01.T01 MTR_5g031160 28.413 271 147 13 6 236 4 267 1.04e-17 80.9
MsG0180005060.01.T01 MTR_5g031030 32.189 233 119 10 34 238 35 256 7.85e-17 78.2
MsG0180005060.01.T01 MTR_5g031140 31.760 233 125 13 31 238 45 268 8.57e-17 78.2
MsG0180005060.01.T01 MTR_5g031090 30.638 235 124 9 34 238 32 257 1.63e-16 77.4
MsG0180005060.01.T01 MTR_4g093080 31.621 253 139 11 18 249 9 248 5.88e-16 77.4
MsG0180005060.01.T01 MTR_8g067960 32.195 205 114 8 54 238 24 223 3.54e-14 70.1
MsG0180005060.01.T01 MTR_4g056320 33.197 244 126 11 1 237 1 214 2.75e-12 65.1
MsG0180005060.01.T01 MTR_4g093040 35.714 140 78 5 111 238 31 170 1.49e-11 64.3
MsG0180005060.01.T01 MTR_4g093040 35.714 140 78 5 111 238 113 252 1.65e-11 64.3
MsG0180005060.01.T01 MTR_4g056360 30.522 249 132 11 1 241 1 216 6.81e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005060.01.T01 AT5G10530 33.071 254 114 12 23 243 10 240 8.66e-18 82.8

Find 71 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATCAGTTGGAGCGTTTC+CGG 0.208844 1:+86365529 None:intergenic
AATATGGTACGTCATAGATT+TGG 0.267899 1:+86365367 None:intergenic
GAAGGAAATGTCAGAGCATT+AGG 0.331482 1:+86366787 None:intergenic
ATCTTAACCCCACCCGTGTC+AGG 0.337146 1:-86366658 MsG0180005060.01.T01:CDS
AATGCTTACAATCAATTTGT+TGG 0.337940 1:-86365425 MsG0180005060.01.T01:CDS
ACCTCGTACTTGTGACCATT+AGG 0.341243 1:-86364683 None:intergenic
AAATCTATGACGTACCATAT+TGG 0.365039 1:-86365365 MsG0180005060.01.T01:CDS
AACAACACTCAATGTGTTAG+AGG 0.369181 1:+86365250 None:intergenic
AGTTGACGTTGGTTCCAATA+TGG 0.371704 1:+86365351 None:intergenic
AATGCTCTGACATTTCCTTC+TGG 0.381773 1:-86366784 MsG0180005060.01.T01:CDS
TTTCTCTCAAGGTCATGAAA+TGG 0.388433 1:-86365322 MsG0180005060.01.T01:CDS
GATCATAAGGATTGGAATAA+AGG 0.392713 1:+86365390 None:intergenic
GTCATAGATTTGGGATCATA+AGG 0.397274 1:+86365377 None:intergenic
ACGGTTAGGTTTGGTATATC+TGG 0.400648 1:-86365158 MsG0180005060.01.T01:CDS
AACTCAGAAAGTGCACGTTT+TGG 0.408486 1:-86365464 MsG0180005060.01.T01:CDS
ATATGGTACGTCATAGATTT+GGG 0.409396 1:+86365368 None:intergenic
ATACCAAACCTAACCGTGTT+GGG 0.410779 1:+86365164 None:intergenic
CCCGTGTCAGGGGCGGAGCT+AGG 0.425624 1:-86366646 MsG0180005060.01.T01:CDS
CTGTACTCCCCAACACGGTT+AGG 0.429988 1:-86365172 MsG0180005060.01.T01:CDS
TTCTCTCAAGGTCATGAAAT+GGG 0.430289 1:-86365321 MsG0180005060.01.T01:CDS
AAGCGTGAGAAAACTCCTTC+AGG 0.438172 1:-86366706 MsG0180005060.01.T01:CDS
ACCTAGCTCCGCCCCTGACA+CGG 0.439457 1:+86366645 None:intergenic
CTCCCCAACACGGTTAGGTT+TGG 0.440223 1:-86365167 MsG0180005060.01.T01:CDS
ATGCTCAAACGTTAAACAAT+GGG 0.443162 1:-86366744 MsG0180005060.01.T01:CDS
CCTCGTACTTGTGACCATTA+GGG 0.449841 1:-86364682 None:intergenic
AAACGTGCACTTTCTGAGTT+GGG 0.453097 1:+86365467 None:intergenic
ACTTGGTGAAATTGAAAGAA+AGG 0.469933 1:+86366815 None:intergenic
CTCCGCCCCTGACACGGGTG+GGG 0.473777 1:+86366651 None:intergenic
CAGAGCATTAGGAGTGAACT+TGG 0.474972 1:+86366798 None:intergenic
CTTGGTGAAATTGAAAGAAA+GGG 0.478623 1:+86366816 None:intergenic
AAAACGTGCACTTTCTGAGT+TGG 0.481047 1:+86365466 None:intergenic
GCTCCGCCCCTGACACGGGT+GGG 0.482083 1:+86366650 None:intergenic
ACCGGAAACGCTCCAACTGA+TGG 0.487866 1:-86365530 MsG0180005060.01.T01:CDS
CAAATCAACGATTTGAGCAA+TGG 0.492987 1:+86365199 None:intergenic
GTTAAGATACGACCTGTACT+TGG 0.493996 1:+86366673 None:intergenic
CCTTCTGGTATAACCTTCCA+AGG 0.495247 1:-86366769 MsG0180005060.01.T01:CDS
TCTTAACCCCACCCGTGTCA+GGG 0.500420 1:-86366657 MsG0180005060.01.T01:CDS
AGATTTGGGATCATAAGGAT+TGG 0.509017 1:+86365382 None:intergenic
TCAAGGTCATGAAATGGGAA+TGG 0.510357 1:-86365316 MsG0180005060.01.T01:CDS
CCTAGCTCCGCCCCTGACAC+GGG 0.510768 1:+86366646 None:intergenic
TGTGTTAGAGGGAGAGTCAT+AGG 0.513568 1:+86365262 None:intergenic
CCTAAAACAAGTTCATGAGT+TGG 0.516836 1:+86366895 None:intergenic
CAAGGTCATGAAATGGGAAT+GGG 0.518239 1:-86365315 MsG0180005060.01.T01:CDS
CAACTCTTTAATTTCTCTCA+AGG 0.521126 1:-86365333 MsG0180005060.01.T01:CDS
AAGAACATCAATCCATCAGT+TGG 0.521731 1:+86365518 None:intergenic
AGCTCCGCCCCTGACACGGG+TGG 0.529106 1:+86366649 None:intergenic
AACCCCACCCGTGTCAGGGG+CGG 0.535593 1:-86366653 MsG0180005060.01.T01:CDS
CGTTTGAGCATCCCCTTGGA+AGG 0.543808 1:+86366756 None:intergenic
TTAACGTTTGAGCATCCCCT+TGG 0.561443 1:+86366752 None:intergenic
CTTCTGGTATAACCTTCCAA+GGG 0.561608 1:-86366768 MsG0180005060.01.T01:CDS
GATGCTCAAACGTTAAACAA+TGG 0.578620 1:-86366745 MsG0180005060.01.T01:CDS
TATACCAAACCTAACCGTGT+TGG 0.579838 1:+86365163 None:intergenic
TCATTCGTAGTAGAGACCAC+CGG 0.586383 1:-86365548 MsG0180005060.01.T01:intron
AGAAATTAAAGAGTTGACGT+TGG 0.590878 1:+86365340 None:intergenic
ATGAAATGGGAATGGGTGAG+TGG 0.593464 1:-86365308 MsG0180005060.01.T01:CDS
CTTAACCCCACCCGTGTCAG+GGG 0.593852 1:-86366656 MsG0180005060.01.T01:CDS
GGTACACTTAAACCAAGTAC+AGG 0.597115 1:-86366685 MsG0180005060.01.T01:CDS
CCTTGGAAGGTTATACCAGA+AGG 0.602083 1:+86366769 None:intergenic
GGTGGAATCACAGTGTCTTG+TGG 0.603572 1:+86365488 None:intergenic
ATCAGTTGGAGCGTTTCCGG+TGG 0.608436 1:+86365532 None:intergenic
CGTGCACTTTCTGAGTTGGG+TGG 0.613896 1:+86365470 None:intergenic
GCGGAGCTAGGTAGTGATGA+GGG 0.615459 1:-86366634 MsG0180005060.01.T01:intron
TACCAAACCTAACCGTGTTG+GGG 0.618772 1:+86365165 None:intergenic
ACAACACTCAATGTGTTAGA+GGG 0.619552 1:+86365251 None:intergenic
GGCGGAGCTAGGTAGTGATG+AGG 0.627972 1:-86366635 MsG0180005060.01.T01:CDS
GTTGTTGTCACTTATGACAA+TGG 0.638598 1:-86365233 MsG0180005060.01.T01:CDS
CTTGGTTTAAGTGTACCTGA+AGG 0.657510 1:+86366691 None:intergenic
GAAAACTGTACTCCCCAACA+CGG 0.658624 1:-86365177 MsG0180005060.01.T01:CDS
CCCTAATGGTCACAAGTACG+AGG 0.683856 1:+86364682 None:intergenic
TTCTGGTATAACCTTCCAAG+GGG 0.688779 1:-86366767 MsG0180005060.01.T01:CDS
TGCTCAAACGTTAAACAATG+GGG 0.705167 1:-86366743 MsG0180005060.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTTAACATTTAACATA+TGG - Chr1:86365596-86365615 MsG0180005060.01.T01:intron 10.0%
!!! ATTCAAATTTTAAATTAAGA+AGG + Chr1:86365525-86365544 None:intergenic 10.0%
!! AATGTATGTATATATTACTA+TGG - Chr1:86365283-86365302 MsG0180005060.01.T01:CDS 15.0%
!!! ATGAAATTTTATTGATGAAA+AGG + Chr1:86366814-86366833 None:intergenic 15.0%
!!! TCATTTTTTTTTTTGTACTA+CGG - Chr1:86366757-86366776 MsG0180005060.01.T01:CDS 15.0%
!! AATATTTGTCCATTCATATA+TGG + Chr1:86365395-86365414 None:intergenic 20.0%
!! ATATCTTACAAATATTTCCA+TGG + Chr1:86365847-86365866 None:intergenic 20.0%
!! CATAAGTAACTAAGTAATTA+GGG - Chr1:86365256-86365275 MsG0180005060.01.T01:CDS 20.0%
!! CTATAGAGAATATTTATCAT+GGG + Chr1:86365448-86365467 None:intergenic 20.0%
!! GTTATCAAAAGAGATAAATA+GGG + Chr1:86365715-86365734 None:intergenic 20.0%
!! TGTTATCAAAAGAGATAAAT+AGG + Chr1:86365716-86365735 None:intergenic 20.0%
!! TTTCTTTGTAAAATGAAAAG+TGG + Chr1:86365092-86365111 None:intergenic 20.0%
!!! GTGTAAATTTTTAAAACTCA+GGG + Chr1:86365009-86365028 None:intergenic 20.0%
!!! TGTGTAAATTTTTAAAACTC+AGG + Chr1:86365010-86365029 None:intergenic 20.0%
!!! TTATTGTCTTAATAGTTTTC+AGG - Chr1:86366884-86366903 MsG0180005060.01.T01:CDS 20.0%
!!! TTTCTTTTTAAACTAGTAGT+TGG - Chr1:86365181-86365200 MsG0180005060.01.T01:CDS 20.0%
! AAGAGATATTAACTCAAACA+TGG + Chr1:86366707-86366726 None:intergenic 25.0%
! AATGCTTACAATCAATTTGT+TGG - Chr1:86366178-86366197 MsG0180005060.01.T01:intron 25.0%
! ATGGTGTATAATGACATAAA+GGG + Chr1:86365372-86365391 None:intergenic 25.0%
! CCATAAGTAACTAAGTAATT+AGG - Chr1:86365255-86365274 MsG0180005060.01.T01:CDS 25.0%
! CCATGATAAATATTCTCTAT+AGG - Chr1:86365446-86365465 MsG0180005060.01.T01:CDS 25.0%
! CCTAATTACTTAGTTACTTA+TGG + Chr1:86365258-86365277 None:intergenic 25.0%
! CCTATAGAGAATATTTATCA+TGG + Chr1:86365449-86365468 None:intergenic 25.0%
! GTAGAAAATGATAATTGTTG+CGG + Chr1:86365748-86365767 None:intergenic 25.0%
! GTATATATTACTATGGACTT+GGG - Chr1:86365290-86365309 MsG0180005060.01.T01:CDS 25.0%
! TGTATATATTACTATGGACT+TGG - Chr1:86365289-86365308 MsG0180005060.01.T01:CDS 25.0%
!!! CTTAATAGTTTTCAGGTAAA+TGG - Chr1:86366891-86366910 MsG0180005060.01.T01:CDS 25.0%
!!! TTTTTTTTTGTACTACGGTT+TGG - Chr1:86366762-86366781 MsG0180005060.01.T01:CDS 25.0%
AAATCTATGACGTACCATAT+TGG - Chr1:86366238-86366257 MsG0180005060.01.T01:intron 30.0%
AATATGGTACGTCATAGATT+TGG + Chr1:86366239-86366258 None:intergenic 30.0%
ACTCAAACATGGTTCAAATA+TGG + Chr1:86366696-86366715 None:intergenic 30.0%
AGAAATTAAAGAGTTGACGT+TGG + Chr1:86366266-86366285 None:intergenic 30.0%
ATACACCATCCATATATGAA+TGG - Chr1:86365383-86365402 MsG0180005060.01.T01:CDS 30.0%
ATATGGTACGTCATAGATTT+GGG + Chr1:86366238-86366257 None:intergenic 30.0%
ATGCTCAAACGTTAAACAAT+GGG - Chr1:86364859-86364878 MsG0180005060.01.T01:intron 30.0%
CCAATCAACTAGTTAATAAC+AGG + Chr1:86365635-86365654 None:intergenic 30.0%
CCTGTTATTAACTAGTTGAT+TGG - Chr1:86365632-86365651 MsG0180005060.01.T01:intron 30.0%
CTTGGTGAAATTGAAAGAAA+GGG + Chr1:86364790-86364809 None:intergenic 30.0%
GATCATAAGGATTGGAATAA+AGG + Chr1:86366216-86366235 None:intergenic 30.0%
GATGGTGTATAATGACATAA+AGG + Chr1:86365373-86365392 None:intergenic 30.0%
TAAAATGAAAAGTGGATCAG+AGG + Chr1:86365084-86365103 None:intergenic 30.0%
TATGATAATTGATGCATGCA+TGG - Chr1:86366575-86366594 MsG0180005060.01.T01:intron 30.0%
TCTCTTACATGCTAAATGTT+TGG - Chr1:86366721-86366740 MsG0180005060.01.T01:CDS 30.0%
TCTTACAAATATTTCCATGG+TGG + Chr1:86365844-86365863 None:intergenic 30.0%
TTGAAAAGAAATCACGTACA+GGG + Chr1:86366666-86366685 None:intergenic 30.0%
TTTGAAAAGAAATCACGTAC+AGG + Chr1:86366667-86366686 None:intergenic 30.0%
TTTGTCCATTCATATATGGA+TGG + Chr1:86365391-86365410 None:intergenic 30.0%
! ACTTGGTGAAATTGAAAGAA+AGG + Chr1:86364791-86364810 None:intergenic 30.0%
! CAACTCTTTAATTTCTCTCA+AGG - Chr1:86366270-86366289 MsG0180005060.01.T01:intron 30.0%
!! CACTTTTTGACAGAAAACAT+TGG - Chr1:86365337-86365356 MsG0180005060.01.T01:CDS 30.0%
AAAGAAGTGAAGAAGAAGTG+AGG - Chr1:86365129-86365148 MsG0180005060.01.T01:intron 35.0%
AACAACACTCAATGTGTTAG+AGG + Chr1:86366356-86366375 None:intergenic 35.0%
AAGAACATCAATCCATCAGT+TGG + Chr1:86366088-86366107 None:intergenic 35.0%
ACAACACTCAATGTGTTAGA+GGG + Chr1:86366355-86366374 None:intergenic 35.0%
CAAATCAACGATTTGAGCAA+TGG + Chr1:86366407-86366426 None:intergenic 35.0%
CAACCATGATAAAAGAAGTG+TGG - Chr1:86365204-86365223 MsG0180005060.01.T01:CDS 35.0%
CCTAAAACAAGTTCATGAGT+TGG + Chr1:86364711-86364730 None:intergenic 35.0%
GATAAGGTTGAACACATTAG+AGG + Chr1:86365795-86365814 None:intergenic 35.0%
GATGCTCAAACGTTAAACAA+TGG - Chr1:86364858-86364877 MsG0180005060.01.T01:intron 35.0%
GTGCAAAGTGCAAATAACAT+AGG + Chr1:86365039-86365058 None:intergenic 35.0%
TGCTCAAACGTTAAACAATG+GGG - Chr1:86364860-86364879 MsG0180005060.01.T01:intron 35.0%
TTCTCTCAAGGTCATGAAAT+GGG - Chr1:86366282-86366301 MsG0180005060.01.T01:intron 35.0%
TTTCTCTCAAGGTCATGAAA+TGG - Chr1:86366281-86366300 MsG0180005060.01.T01:intron 35.0%
! AGATTTGGGATCATAAGGAT+TGG + Chr1:86366224-86366243 None:intergenic 35.0%
! GTCATAGATTTGGGATCATA+AGG + Chr1:86366229-86366248 None:intergenic 35.0%
! GTTGTTGTCACTTATGACAA+TGG - Chr1:86366370-86366389 MsG0180005060.01.T01:intron 35.0%
! TATAGCTTGTAACTTGTCTG+AGG + Chr1:86366555-86366574 None:intergenic 35.0%
!! CCAACTCATGAACTTGTTTT+AGG - Chr1:86364708-86364727 MsG0180005060.01.T01:intron 35.0%
!!! AGGTGCAAACAGATTTTTTC+AGG + Chr1:86365064-86365083 None:intergenic 35.0%
!!! GGTGCAAACAGATTTTTTCA+GGG + Chr1:86365063-86365082 None:intergenic 35.0%
AAAACGTGCACTTTCTGAGT+TGG + Chr1:86366140-86366159 None:intergenic 40.0%
AAACGTGCACTTTCTGAGTT+GGG + Chr1:86366139-86366158 None:intergenic 40.0%
AATGCTCTGACATTTCCTTC+TGG - Chr1:86364819-86364838 MsG0180005060.01.T01:intron 40.0%
ATACCAAACCTAACCGTGTT+GGG + Chr1:86366442-86366461 None:intergenic 40.0%
ATATCTGGTGCTTCAGTAAC+TGG - Chr1:86366460-86366479 MsG0180005060.01.T01:intron 40.0%
CAAGGTCATGAAATGGGAAT+GGG - Chr1:86366288-86366307 MsG0180005060.01.T01:intron 40.0%
CTTCTGGTATAACCTTCCAA+GGG - Chr1:86364835-86364854 MsG0180005060.01.T01:intron 40.0%
CTTGGTTTAAGTGTACCTGA+AGG + Chr1:86364915-86364934 None:intergenic 40.0%
GGTACACTTAAACCAAGTAC+AGG - Chr1:86364918-86364937 MsG0180005060.01.T01:intron 40.0%
GGTCTCTACTACGAATGAAA+AGG + Chr1:86366053-86366072 None:intergenic 40.0%
GTTAAGATACGACCTGTACT+TGG + Chr1:86364933-86364952 None:intergenic 40.0%
TATACCAAACCTAACCGTGT+TGG + Chr1:86366443-86366462 None:intergenic 40.0%
TCAAGGTCATGAAATGGGAA+TGG - Chr1:86366287-86366306 MsG0180005060.01.T01:intron 40.0%
TTCTGGTATAACCTTCCAAG+GGG - Chr1:86364836-86364855 MsG0180005060.01.T01:intron 40.0%
! ACGGTTAGGTTTGGTATATC+TGG - Chr1:86366445-86366464 MsG0180005060.01.T01:intron 40.0%
! AGGCCACACTTCTTTTATCA+TGG + Chr1:86365210-86365229 None:intergenic 40.0%
! ATGATAAAAGAAGTGTGGCC+TGG - Chr1:86365209-86365228 MsG0180005060.01.T01:CDS 40.0%
! GAAGGAAATGTCAGAGCATT+AGG + Chr1:86364819-86364838 None:intergenic 40.0%
!! AACTCAGAAAGTGCACGTTT+TGG - Chr1:86366139-86366158 MsG0180005060.01.T01:intron 40.0%
!! AAGTGCTGAATGACCATGAA+TGG + Chr1:86366501-86366520 None:intergenic 40.0%
!! AGTTGACGTTGGTTCCAATA+TGG + Chr1:86366255-86366274 None:intergenic 40.0%
!!! TTTTAAAACTCAGGGTGTGC+AGG + Chr1:86365001-86365020 None:intergenic 40.0%
AAGCGTGAGAAAACTCCTTC+AGG - Chr1:86364897-86364916 MsG0180005060.01.T01:intron 45.0%
ACACAAGCTGCTTACTAGCA+GGG + Chr1:86366641-86366660 None:intergenic 45.0%
ATGAAATGGGAATGGGTGAG+TGG - Chr1:86366295-86366314 MsG0180005060.01.T01:intron 45.0%
CAGCACATGACATCCATTCA+TGG - Chr1:86366485-86366504 MsG0180005060.01.T01:intron 45.0%
CCTTCTGGTATAACCTTCCA+AGG - Chr1:86364834-86364853 MsG0180005060.01.T01:intron 45.0%
CCTTGGAAGGTTATACCAGA+AGG + Chr1:86364837-86364856 None:intergenic 45.0%
GAAAACTGTACTCCCCAACA+CGG - Chr1:86366426-86366445 MsG0180005060.01.T01:intron 45.0%
GACAAAGCTAGCAACATTGC+CGG + Chr1:86366026-86366045 None:intergenic 45.0%
TACCAAACCTAACCGTGTTG+GGG + Chr1:86366441-86366460 None:intergenic 45.0%
TCATTCGTAGTAGAGACCAC+CGG - Chr1:86366055-86366074 MsG0180005060.01.T01:intron 45.0%
TGTGTTAGAGGGAGAGTCAT+AGG + Chr1:86366344-86366363 None:intergenic 45.0%
TTAACGTTTGAGCATCCCCT+TGG + Chr1:86364854-86364873 None:intergenic 45.0%
! CAGAGCATTAGGAGTGAACT+TGG + Chr1:86364808-86364827 None:intergenic 45.0%
!! TTCCATGGTGGATCGAGTAT+GGG + Chr1:86365832-86365851 None:intergenic 45.0%
!! TTTCCATGGTGGATCGAGTA+TGG + Chr1:86365833-86365852 None:intergenic 45.0%
CACACAAGCTGCTTACTAGC+AGG + Chr1:86366642-86366661 None:intergenic 50.0%
GCTAAATTGTTTGCACCCCC+TGG - Chr1:86365953-86365972 MsG0180005060.01.T01:intron 50.0%
GGGCAATGTAGAACGCGATA+AGG + Chr1:86365811-86365830 None:intergenic 50.0%
GGTGGAATCACAGTGTCTTG+TGG + Chr1:86366118-86366137 None:intergenic 50.0%
! TCCATCAGTTGGAGCGTTTC+CGG + Chr1:86366077-86366096 None:intergenic 50.0%
!! TCCATGGTGGATCGAGTATG+GGG + Chr1:86365831-86365850 None:intergenic 50.0%
ACCGGAAACGCTCCAACTGA+TGG - Chr1:86366073-86366092 MsG0180005060.01.T01:intron 55.0%
ATCTTAACCCCACCCGTGTC+AGG - Chr1:86364945-86364964 MsG0180005060.01.T01:intron 55.0%
ATTGCCGGCAGTGTCCCAAA+GGG + Chr1:86366011-86366030 None:intergenic 55.0%
CGTTTGAGCATCCCCTTGGA+AGG + Chr1:86364850-86364869 None:intergenic 55.0%
CTCCCCAACACGGTTAGGTT+TGG - Chr1:86366436-86366455 MsG0180005060.01.T01:intron 55.0%
CTGTACTCCCCAACACGGTT+AGG - Chr1:86366431-86366450 MsG0180005060.01.T01:intron 55.0%
GAAGAAGTGAGGCACGACTG+AGG - Chr1:86365140-86365159 MsG0180005060.01.T01:intron 55.0%
GCGGAGCTAGGTAGTGATGA+GGG - Chr1:86364969-86364988 MsG0180005060.01.T01:intron 55.0%
TCTTAACCCCACCCGTGTCA+GGG - Chr1:86364946-86364965 MsG0180005060.01.T01:intron 55.0%
! ATCAGTTGGAGCGTTTCCGG+TGG + Chr1:86366074-86366093 None:intergenic 55.0%
! CGTGCACTTTCTGAGTTGGG+TGG + Chr1:86366136-86366155 None:intergenic 55.0%
AAGTGTGGCCTGGCTGTGTG+TGG - Chr1:86365219-86365238 MsG0180005060.01.T01:CDS 60.0%
AGTGGCGTATCCAGGACCCT+GGG + Chr1:86365979-86365998 None:intergenic 60.0%
CATTGCCGGCAGTGTCCCAA+AGG + Chr1:86366012-86366031 None:intergenic 60.0%
CCACTGACCCGTGTCCCTTT+GGG - Chr1:86365994-86366013 MsG0180005060.01.T01:intron 60.0%
CTTAACCCCACCCGTGTCAG+GGG - Chr1:86364947-86364966 MsG0180005060.01.T01:intron 60.0%
GCAGTGTCCCAAAGGGACAC+GGG + Chr1:86366004-86366023 None:intergenic 60.0%
GCCCCATACTCGATCCACCA+TGG - Chr1:86365827-86365846 MsG0180005060.01.T01:intron 60.0%
GGCAGTGTCCCAAAGGGACA+CGG + Chr1:86366005-86366024 None:intergenic 60.0%
GGCGGAGCTAGGTAGTGATG+AGG - Chr1:86364968-86364987 MsG0180005060.01.T01:intron 60.0%
!! GTGTCCCTTTGGGACACTGC+CGG - Chr1:86366004-86366023 MsG0180005060.01.T01:intron 60.0%
ACCTAGCTCCGCCCCTGACA+CGG + Chr1:86364961-86364980 None:intergenic 65.0%
CACGGGTCAGTGGCGTATCC+AGG + Chr1:86365987-86366006 None:intergenic 65.0%
CAGTGGCGTATCCAGGACCC+TGG + Chr1:86365980-86365999 None:intergenic 65.0%
CCCAAAGGGACACGGGTCAG+TGG + Chr1:86365997-86366016 None:intergenic 65.0%
GCCACTGACCCGTGTCCCTT+TGG - Chr1:86365993-86366012 MsG0180005060.01.T01:intron 65.0%
GTATCCAGGACCCTGGGCCA+GGG + Chr1:86365973-86365992 None:intergenic 65.0%
TATCCAGGACCCTGGGCCAG+GGG + Chr1:86365972-86365991 None:intergenic 65.0%
TGTGGCCTGGCTGTGTGTGG+CGG - Chr1:86365222-86365241 MsG0180005060.01.T01:CDS 65.0%
TGTTTGCACCCCCTGGCCCA+GGG - Chr1:86365960-86365979 MsG0180005060.01.T01:intron 65.0%
TTGTTTGCACCCCCTGGCCC+AGG - Chr1:86365959-86365978 MsG0180005060.01.T01:intron 65.0%
AACCCCACCCGTGTCAGGGG+CGG - Chr1:86364950-86364969 MsG0180005060.01.T01:intron 70.0%
AGCAGCCGCCACACACAGCC+AGG + Chr1:86365230-86365249 None:intergenic 70.0%
ATCCAGGACCCTGGGCCAGG+GGG + Chr1:86365971-86365990 None:intergenic 70.0%
CCTAGCTCCGCCCCTGACAC+GGG + Chr1:86364960-86364979 None:intergenic 70.0%
CGTATCCAGGACCCTGGGCC+AGG + Chr1:86365974-86365993 None:intergenic 70.0%
AGCTCCGCCCCTGACACGGG+TGG + Chr1:86364957-86364976 None:intergenic 75.0%
CCCGTGTCAGGGGCGGAGCT+AGG - Chr1:86364957-86364976 MsG0180005060.01.T01:intron 75.0%
CTCCGCCCCTGACACGGGTG+GGG + Chr1:86364955-86364974 None:intergenic 75.0%
GCTCCGCCCCTGACACGGGT+GGG + Chr1:86364956-86364975 None:intergenic 75.0%
! CACCCCCTGGCCCAGGGTCC+TGG - Chr1:86365966-86365985 MsG0180005060.01.T01:intron 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 86364684 86366941 86364684 ID=MsG0180005060.01;Name=MsG0180005060.01
Chr1 mRNA 86364684 86366941 86364684 ID=MsG0180005060.01.T01;Parent=MsG0180005060.01;Name=MsG0180005060.01.T01;_AED=0.48;_eAED=0.51;_QI=0|0|0|1|0|0|3|0|249
Chr1 exon 86366635 86366941 86366635 ID=MsG0180005060.01.T01:exon:28445;Parent=MsG0180005060.01.T01
Chr1 exon 86365151 86365557 86365151 ID=MsG0180005060.01.T01:exon:28444;Parent=MsG0180005060.01.T01
Chr1 exon 86364684 86364719 86364684 ID=MsG0180005060.01.T01:exon:28443;Parent=MsG0180005060.01.T01
Chr1 CDS 86366635 86366941 86366635 ID=MsG0180005060.01.T01:cds;Parent=MsG0180005060.01.T01
Chr1 CDS 86365151 86365557 86365151 ID=MsG0180005060.01.T01:cds;Parent=MsG0180005060.01.T01
Chr1 CDS 86364684 86364719 86364684 ID=MsG0180005060.01.T01:cds;Parent=MsG0180005060.01.T01
Gene Sequence

>MsG0180005060.01.T01

ATGGCTTTTTATCGCACAAATCTTCCAACTCATGAACTTGTTTTAGGCATAATATACACAACTTTCTTTCTCTTGCTAGCTACAAATATAAACTCAGTACAAGCCCTTTCTTTCAATTTCACCAAGTTCACTCCTAATGCTCTGACATTTCCTTCTGGTATAACCTTCCAAGGGGATGCTCAAACGTTAAACAATGGGGTCATCGCACTGACTAAGCGTGAGAAAACTCCTTCAGGTACACTTAAACCAAGTACAGGTCGTATCTTAACCCCACCCGTGTCAGGGGCGGAGCTAGGTAGTGATGAGGAGACCACCGGAAACGCTCCAACTGATGGATTGATGTTCTTTATTGCACCACAAGACACTGTGATTCCACCCAACTCAGAAAGTGCACGTTTTGGAGTAGTTGATTCAAAAAATGCTTACAATCAATTTGTTGGTGTAGAGTTTGACCTTTATTCCAATCCTTATGATCCCAAATCTATGACGTACCATATTGGAACCAACGTCAACTCTTTAATTTCTCTCAAGGTCATGAAATGGGAATGGGTGAGTGGATCATTGAATGAAGTAAGTATAGCCTATGACTCTCCCTCTAACACATTGAGTGTTGTTGTCACTTATGACAATGGTAAATTTTCTACCATTGCTCAAATCGTTGATTTGAAAACTGTACTCCCCAACACGGTTAGGTTTGGTATATCTGGTGCTTCAGTAAATGGTATCACACCTCGTACTTGTGACCATTAG
Protein sequence

>MsG0180005060.01.T01

MAFYRTNLPTHELVLGIIYTTFFLLLATNINSVQALSFNFTKFTPNALTFPSGITFQGDAQTLNNGVIALTKREKTPSGTLKPSTGRILTPPVSGAELGSDEETTGNAPTDGLMFFIAPQDTVIPPNSESARFGVVDSKNAYNQFVGVEFDLYSNPYDPKSMTYHIGTNVNSLISLKVMKWEWVSGSLNEVSIAYDSPSNTLSVVVTYDNGKFSTIAQIVDLKTVLPNTVRFGISGASVNGITPRTCDH*