Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013439.01.T01 | RHN66718.1 | 96.445 | 422 | 15 | 0 | 1 | 422 | 22 | 443 | 0 | 844 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013439.01.T01 | A0A396IPR0 | 96.445 | 422 | 15 | 0 | 1 | 422 | 22 | 443 | 0.0 | 844 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000116.01 | MsG0380013439.01 | 0.814528 | 1.471763e-51 | 9.899650e-49 |
| MsG0280011334.01 | MsG0380013439.01 | 0.816472 | 5.434895e-52 | 3.854490e-49 |
| MsG0380013439.01 | MsG0480023165.01 | 0.806689 | 7.282885e-50 | 3.978283e-47 |
| MsG0380013439.01 | MsG0480023670.01 | 0.808249 | 3.397652e-50 | 1.933197e-47 |
| MsG0380013439.01 | MsG0580024157.01 | 0.816256 | 6.074636e-52 | 4.283206e-49 |
| MsG0380013439.01 | MsG0580025206.01 | 0.800523 | 1.384782e-48 | 6.461779e-46 |
| MsG0380013439.01 | MsG0680030656.01 | 0.801810 | 7.553450e-49 | 3.641397e-46 |
| MsG0380013439.01 | MsG0680030677.01 | 0.800567 | 1.356276e-48 | 6.335871e-46 |
| MsG0380013439.01 | MsG0680035764.01 | 0.803009 | 4.276735e-49 | 2.125514e-46 |
| MsG0380013439.01 | MsG0780038117.01 | 0.808661 | 2.775598e-50 | 1.596213e-47 |
| MsG0380013439.01 | MsG0780040669.01 | 0.811552 | 6.612735e-51 | 4.105692e-48 |
| MsG0380013439.01 | MsG0780041764.01 | 0.807968 | 3.900675e-50 | 2.202810e-47 |
| MsG0380013439.01 | MsG0880042698.01 | 0.819457 | 1.149853e-52 | 8.853625e-50 |
| MsG0280009754.01 | MsG0380013439.01 | 0.804431 | 2.167112e-49 | 1.117146e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380014388.01 | MsG0380013439.01 | PPI |
| MsG0380013439.01 | MsG0580029897.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013439.01.T01 | MTR_5g089720 | 62.850 | 428 | 130 | 8 | 8 | 422 | 26 | 437 | 3.64e-174 | 494 |
| MsG0380013439.01.T01 | MTR_5g094340 | 36.891 | 431 | 184 | 14 | 14 | 422 | 53 | 417 | 5.66e-75 | 240 |
| MsG0380013439.01.T01 | MTR_3g055960 | 49.738 | 191 | 93 | 2 | 14 | 201 | 54 | 244 | 5.94e-58 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013439.01.T01 | AT5G12300 | 64.677 | 201 | 57 | 4 | 15 | 206 | 19 | 214 | 4.18e-84 | 262 |
| MsG0380013439.01.T01 | AT1G50570 | 35.714 | 420 | 190 | 8 | 15 | 422 | 37 | 388 | 1.62e-70 | 228 |
| MsG0380013439.01.T01 | AT1G50570 | 35.714 | 420 | 190 | 8 | 15 | 422 | 37 | 388 | 1.62e-70 | 228 |
| MsG0380013439.01.T01 | AT5G55530 | 53.403 | 191 | 80 | 3 | 17 | 201 | 51 | 238 | 9.36e-63 | 208 |
| MsG0380013439.01.T01 | AT5G55530 | 53.403 | 191 | 80 | 3 | 17 | 201 | 51 | 238 | 9.36e-63 | 208 |
| MsG0380013439.01.T01 | AT5G55530 | 53.403 | 191 | 80 | 3 | 17 | 201 | 51 | 238 | 9.36e-63 | 208 |
Find 44 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTGGAAGTTGGAGTATC+TGG | 0.194020 | 3:-38031763 | None:intergenic |
| CATTATCATTATCATCTAAA+TGG | 0.258372 | 3:-38030886 | None:intergenic |
| CTTCTTATCGGTCTCTAATC+TGG | 0.289838 | 3:-38032079 | None:intergenic |
| GCATCAATTCACCGAGTCTT+TGG | 0.292100 | 3:+38031975 | MsG0380013439.01.T01:CDS |
| AATCTTATGGTTCTGTTTCA+AGG | 0.305198 | 3:+38031485 | MsG0380013439.01.T01:CDS |
| TACTTCGATTTGGAATCTTA+TGG | 0.311795 | 3:+38031472 | MsG0380013439.01.T01:CDS |
| TCATTAGCCGCATTGATATC+AGG | 0.322487 | 3:-38031427 | None:intergenic |
| GAACTCAAAGTCGAATTGAT+TGG | 0.346252 | 3:-38031322 | None:intergenic |
| GTTTCAAGGCCTAATTCAAT+AGG | 0.354760 | 3:+38031499 | MsG0380013439.01.T01:CDS |
| TCTAGTTTCGCTTTCTTCCT+TGG | 0.365371 | 3:-38031779 | None:intergenic |
| GATTATCATGATTGTGATCA+AGG | 0.365568 | 3:-38032020 | None:intergenic |
| GGCAACGATCCTATTGAATT+AGG | 0.380609 | 3:-38031508 | None:intergenic |
| GGTTTGTGAGTACTTCGATT+TGG | 0.381118 | 3:+38031462 | MsG0380013439.01.T01:CDS |
| AGAATCATGAGTCCATTTCT+AGG | 0.384976 | 3:+38031596 | MsG0380013439.01.T01:CDS |
| GGCTAATGAGAATCAGAAAA+TGG | 0.389369 | 3:+38031441 | MsG0380013439.01.T01:CDS |
| TCTACAAGAATCATCAATAA+AGG | 0.390795 | 3:+38031043 | MsG0380013439.01.T01:CDS |
| AATATAGAAGCTGCAAAATT+CGG | 0.391192 | 3:+38031859 | MsG0380013439.01.T01:CDS |
| TCAATTCTAGAATACTCAAT+TGG | 0.410165 | 3:-38031400 | None:intergenic |
| CTTGTAGAGAGCGTTTCATC+AGG | 0.417624 | 3:-38031028 | None:intergenic |
| TCCGCCACAAAATATCGATA+TGG | 0.421403 | 3:+38031897 | MsG0380013439.01.T01:CDS |
| AGAGCTTGAAAACCTAGAAA+TGG | 0.425833 | 3:-38031608 | None:intergenic |
| TTTGAGTTCGAAACCACAAT+CGG | 0.438281 | 3:+38031336 | MsG0380013439.01.T01:CDS |
| AACTGAGACTAACACATTGA+AGG | 0.447563 | 3:-38031713 | None:intergenic |
| AAAACGAGACGAATATTGAT+AGG | 0.461835 | 3:+38031836 | MsG0380013439.01.T01:CDS |
| TCGCTTTCTTCCTTGGAAGT+TGG | 0.466636 | 3:-38031772 | None:intergenic |
| TTGCCGTTTCTTCAACTTAG+CGG | 0.469096 | 3:+38031526 | MsG0380013439.01.T01:CDS |
| AATCATGATAATCAAAATCA+AGG | 0.499360 | 3:+38032030 | MsG0380013439.01.T01:CDS |
| TGATGTAATTCAAAATCATA+AGG | 0.500874 | 3:+38032053 | MsG0380013439.01.T01:CDS |
| GTTAATTGAACAGTTCCAGC+TGG | 0.526635 | 3:-38031277 | None:intergenic |
| GAACCGCTAAGTTGAAGAAA+CGG | 0.537620 | 3:-38031529 | None:intergenic |
| GAAATGACAATGAATTCGTC+AGG | 0.546995 | 3:+38031571 | MsG0380013439.01.T01:CDS |
| TCCAGATACTCCAACTTCCA+AGG | 0.556108 | 3:+38031762 | MsG0380013439.01.T01:CDS |
| TTGAGTTCGAAACCACAATC+GGG | 0.560282 | 3:+38031337 | MsG0380013439.01.T01:CDS |
| TGATTGTGATCAAGGTCCAT+TGG | 0.563994 | 3:-38032012 | None:intergenic |
| ATTTCACAGTCAACATCTTG+AGG | 0.577410 | 3:-38031553 | None:intergenic |
| ACAAGAATCATCAATAAAGG+TGG | 0.583060 | 3:+38031046 | MsG0380013439.01.T01:CDS |
| GATGAATTAGTACCCGATTG+TGG | 0.591123 | 3:-38031349 | None:intergenic |
| GGCGAAAATGAAGCTACCAA+TGG | 0.599527 | 3:+38031996 | MsG0380013439.01.T01:CDS |
| TTGTAGAGAGCGTTTCATCA+GGG | 0.604243 | 3:-38031027 | None:intergenic |
| GCCACAAAATATCGATATGG+AGG | 0.626756 | 3:+38031900 | MsG0380013439.01.T01:CDS |
| GGAGTTAAAGTTCTCAACCA+AGG | 0.638594 | 3:+38031813 | MsG0380013439.01.T01:CDS |
| GCGATAAAGAGTAATTTGAG+AGG | 0.647851 | 3:+38031676 | MsG0380013439.01.T01:CDS |
| AGCGATGATAGAAACACGAA+CGG | 0.692429 | 3:+38031646 | MsG0380013439.01.T01:CDS |
| TGAATTTCCTGATATCAATG+CGG | 0.723355 | 3:+38031420 | MsG0380013439.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTCAATCAAAATTTAA+GGG | + | Chr3:38031078-38031097 | MsG0380013439.01.T01:CDS | 10.0% |
| !!! | CAATTTTCAATCAAAATTTA+AGG | + | Chr3:38031077-38031096 | MsG0380013439.01.T01:CDS | 15.0% |
| !!! | CTTAAATTTTGATTGAAAAT+TGG | - | Chr3:38031079-38031098 | None:intergenic | 15.0% |
| !! | AATCATGATAATCAAAATCA+AGG | + | Chr3:38032030-38032049 | MsG0380013439.01.T01:CDS | 20.0% |
| !! | CATTATCATTATCATCTAAA+TGG | - | Chr3:38030889-38030908 | None:intergenic | 20.0% |
| !! | TGATGTAATTCAAAATCATA+AGG | + | Chr3:38032053-38032072 | MsG0380013439.01.T01:CDS | 20.0% |
| !!! | ATTTCTAAAGTTTTTGGTAA+AGG | + | Chr3:38031205-38031224 | MsG0380013439.01.T01:CDS | 20.0% |
| !!! | TTACCAAAAACTTTAGAAAT+TGG | - | Chr3:38031205-38031224 | None:intergenic | 20.0% |
| ! | AATATAGAAGCTGCAAAATT+CGG | + | Chr3:38031859-38031878 | MsG0380013439.01.T01:CDS | 25.0% |
| ! | TCTACAAGAATCATCAATAA+AGG | + | Chr3:38031043-38031062 | MsG0380013439.01.T01:CDS | 25.0% |
| !! | ACTTAGAAGATCAACTTTTA+GGG | + | Chr3:38031167-38031186 | MsG0380013439.01.T01:CDS | 25.0% |
| !!! | GTTCCAATTTCTAAAGTTTT+TGG | + | Chr3:38031199-38031218 | MsG0380013439.01.T01:CDS | 25.0% |
| !!! | TCAATTCTAGAATACTCAAT+TGG | - | Chr3:38031403-38031422 | None:intergenic | 25.0% |
| AAAACGAGACGAATATTGAT+AGG | + | Chr3:38031836-38031855 | MsG0380013439.01.T01:CDS | 30.0% | |
| ACAAGAATCATCAATAAAGG+TGG | + | Chr3:38031046-38031065 | MsG0380013439.01.T01:CDS | 30.0% | |
| ACCGATAAGAAGAAAAATGA+CGG | + | Chr3:38032090-38032109 | MsG0380013439.01.T01:CDS | 30.0% | |
| CGAAACTAGAGATGAAAAAA+AGG | + | Chr3:38031792-38031811 | MsG0380013439.01.T01:CDS | 30.0% | |
| GATTATCATGATTGTGATCA+AGG | - | Chr3:38032023-38032042 | None:intergenic | 30.0% | |
| TGAATTTCCTGATATCAATG+CGG | + | Chr3:38031420-38031439 | MsG0380013439.01.T01:CDS | 30.0% | |
| ! | AATCTTATGGTTCTGTTTCA+AGG | + | Chr3:38031485-38031504 | MsG0380013439.01.T01:CDS | 30.0% |
| ! | CACTTAGAAGATCAACTTTT+AGG | + | Chr3:38031166-38031185 | MsG0380013439.01.T01:CDS | 30.0% |
| !! | TACTTCGATTTGGAATCTTA+TGG | + | Chr3:38031472-38031491 | MsG0380013439.01.T01:CDS | 30.0% |
| !!! | CTGTTTTAAAATGTGAGCTT+TGG | + | Chr3:38031122-38031141 | MsG0380013439.01.T01:CDS | 30.0% |
| AACTGAGACTAACACATTGA+AGG | - | Chr3:38031716-38031735 | None:intergenic | 35.0% | |
| AGAATCATGAGTCCATTTCT+AGG | + | Chr3:38031596-38031615 | MsG0380013439.01.T01:CDS | 35.0% | |
| AGAGCTTGAAAACCTAGAAA+TGG | - | Chr3:38031611-38031630 | None:intergenic | 35.0% | |
| ATTTCACAGTCAACATCTTG+AGG | - | Chr3:38031556-38031575 | None:intergenic | 35.0% | |
| CCGATAAGAAGAAAAATGAC+GGG | + | Chr3:38032091-38032110 | MsG0380013439.01.T01:CDS | 35.0% | |
| GAAATGACAATGAATTCGTC+AGG | + | Chr3:38031571-38031590 | MsG0380013439.01.T01:CDS | 35.0% | |
| GAACTCAAAGTCGAATTGAT+TGG | - | Chr3:38031325-38031344 | None:intergenic | 35.0% | |
| GCGATAAAGAGTAATTTGAG+AGG | + | Chr3:38031676-38031695 | MsG0380013439.01.T01:CDS | 35.0% | |
| GGCTAATGAGAATCAGAAAA+TGG | + | Chr3:38031441-38031460 | MsG0380013439.01.T01:CDS | 35.0% | |
| GTTTCAAGGCCTAATTCAAT+AGG | + | Chr3:38031499-38031518 | MsG0380013439.01.T01:CDS | 35.0% | |
| TGGAGAATGAAAAAGATCAG+TGG | - | Chr3:38031260-38031279 | None:intergenic | 35.0% | |
| ! | AATATTCGTCTCGTTTTCCT+TGG | - | Chr3:38031833-38031852 | None:intergenic | 35.0% |
| ! | TCCATATCGATATTTTGTGG+CGG | - | Chr3:38031901-38031920 | None:intergenic | 35.0% |
| ! | TTTGAGTTCGAAACCACAAT+CGG | + | Chr3:38031336-38031355 | MsG0380013439.01.T01:CDS | 35.0% |
| !! | GATGATGATGTTGAGTTTTC+AGG | + | Chr3:38030908-38030927 | MsG0380013439.01.T01:CDS | 35.0% |
| !!! | CCCGTCATTTTTCTTCTTAT+CGG | - | Chr3:38032094-38032113 | None:intergenic | 35.0% |
| !!! | TCAACTTTTAGGGTTTGCTT+TGG | + | Chr3:38031177-38031196 | MsG0380013439.01.T01:CDS | 35.0% |
| AGCGATGATAGAAACACGAA+CGG | + | Chr3:38031646-38031665 | MsG0380013439.01.T01:CDS | 40.0% | |
| CTTCTTATCGGTCTCTAATC+TGG | - | Chr3:38032082-38032101 | None:intergenic | 40.0% | |
| GATGAATTAGTACCCGATTG+TGG | - | Chr3:38031352-38031371 | None:intergenic | 40.0% | |
| GCCACAAAATATCGATATGG+AGG | + | Chr3:38031900-38031919 | MsG0380013439.01.T01:CDS | 40.0% | |
| GGAGTTAAAGTTCTCAACCA+AGG | + | Chr3:38031813-38031832 | MsG0380013439.01.T01:CDS | 40.0% | |
| GGCAACGATCCTATTGAATT+AGG | - | Chr3:38031511-38031530 | None:intergenic | 40.0% | |
| GGTTTGTGAGTACTTCGATT+TGG | + | Chr3:38031462-38031481 | MsG0380013439.01.T01:CDS | 40.0% | |
| GTTAATTGAACAGTTCCAGC+TGG | - | Chr3:38031280-38031299 | None:intergenic | 40.0% | |
| TCCGCCACAAAATATCGATA+TGG | + | Chr3:38031897-38031916 | MsG0380013439.01.T01:CDS | 40.0% | |
| TGATTGTGATCAAGGTCCAT+TGG | - | Chr3:38032015-38032034 | None:intergenic | 40.0% | |
| TTGAGTTCGAAACCACAATC+GGG | + | Chr3:38031337-38031356 | MsG0380013439.01.T01:CDS | 40.0% | |
| ! | CTTCATTTTCGCCAAAGACT+CGG | - | Chr3:38031989-38032008 | None:intergenic | 40.0% |
| ! | GAACCGCTAAGTTGAAGAAA+CGG | - | Chr3:38031532-38031551 | None:intergenic | 40.0% |
| ! | GCCTCCATATCGATATTTTG+TGG | - | Chr3:38031904-38031923 | None:intergenic | 40.0% |
| ! | TCATTAGCCGCATTGATATC+AGG | - | Chr3:38031430-38031449 | None:intergenic | 40.0% |
| ! | TCTAGTTTCGCTTTCTTCCT+TGG | - | Chr3:38031782-38031801 | None:intergenic | 40.0% |
| ! | TTGCCGTTTCTTCAACTTAG+CGG | + | Chr3:38031526-38031545 | MsG0380013439.01.T01:CDS | 40.0% |
| !! | GATCTTTTTCATTCTCCAGC+TGG | + | Chr3:38031262-38031281 | MsG0380013439.01.T01:CDS | 40.0% |
| !! | TTGTAGAGAGCGTTTCATCA+GGG | - | Chr3:38031030-38031049 | None:intergenic | 40.0% |
| GCATCAATTCACCGAGTCTT+TGG | + | Chr3:38031975-38031994 | MsG0380013439.01.T01:CDS | 45.0% | |
| GGCGAAAATGAAGCTACCAA+TGG | + | Chr3:38031996-38032015 | MsG0380013439.01.T01:CDS | 45.0% | |
| TCCAGATACTCCAACTTCCA+AGG | + | Chr3:38031762-38031781 | MsG0380013439.01.T01:CDS | 45.0% | |
| TCGCTTTCTTCCTTGGAAGT+TGG | - | Chr3:38031775-38031794 | None:intergenic | 45.0% | |
| ! | TCCTTGGAAGTTGGAGTATC+TGG | - | Chr3:38031766-38031785 | None:intergenic | 45.0% |
| !! | CTTGTAGAGAGCGTTTCATC+AGG | - | Chr3:38031031-38031050 | None:intergenic | 45.0% |
| ! | GACGGGTCGCGCGTGTTTTA+TGG | + | Chr3:38032108-38032127 | MsG0380013439.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 38030881 | 38032149 | 38030881 | ID=MsG0380013439.01;Name=MsG0380013439.01 |
| Chr3 | mRNA | 38030881 | 38032149 | 38030881 | ID=MsG0380013439.01.T01;Parent=MsG0380013439.01;Name=MsG0380013439.01.T01;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|422 |
| Chr3 | exon | 38030881 | 38032149 | 38030881 | ID=MsG0380013439.01.T01:exon:26881;Parent=MsG0380013439.01.T01 |
| Chr3 | CDS | 38030881 | 38032149 | 38030881 | ID=MsG0380013439.01.T01:cds;Parent=MsG0380013439.01.T01 |
| Gene Sequence |
| Protein sequence |
