Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013937.01.T01 | KAF3611721.1 | 93.333 | 210 | 14 | 0 | 1 | 210 | 23 | 232 | 1.98E-143 | 409 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013937.01.T01 | P46818 | 93.333 | 210 | 14 | 0 | 1 | 210 | 869 | 1078 | 1.08E-136 | 412 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013937.01.T01 | A0A2Z4KH34 | 99.524 | 210 | 1 | 0 | 1 | 210 | 866 | 1075 | 1.29e-142 | 434 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013937.01 | MsG0380013961.01 | 0.807787 | 4.260681e-50 | 2.394802e-47 |
| MsG0380013937.01 | MsG0380013962.01 | 0.803600 | 3.226178e-49 | 1.628050e-46 |
| MsG0380013937.01 | MsG0380014027.01 | 0.806678 | 7.321237e-50 | 3.998000e-47 |
| MsG0380013937.01 | MsG0380014046.01 | 0.918612 | 1.256479e-86 | 3.861614e-82 |
| MsG0380013937.01 | MsG0380014054.01 | 0.891021 | 5.809844e-74 | 5.007724e-70 |
| MsG0280009733.01 | MsG0380013937.01 | 0.800933 | 1.142142e-48 | 5.385574e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013937.01.T01 | MTR_4g051130 | 99.043 | 209 | 2 | 0 | 1 | 209 | 848 | 1056 | 4.30e-139 | 426 |
| MsG0380013937.01.T01 | MTR_0002s0510 | 82.843 | 204 | 35 | 0 | 1 | 204 | 919 | 1122 | 1.73e-113 | 356 |
| MsG0380013937.01.T01 | MTR_6g061700 | 84.507 | 71 | 11 | 0 | 83 | 153 | 5 | 75 | 2.44e-36 | 124 |
| MsG0380013937.01.T01 | MTR_7g052990 | 88.889 | 45 | 5 | 0 | 132 | 176 | 21 | 65 | 2.12e-19 | 79.0 |
| MsG0380013937.01.T01 | MTR_7g052900 | 86.667 | 45 | 6 | 0 | 132 | 176 | 21 | 65 | 3.65e-18 | 75.5 |
| MsG0380013937.01.T01 | MTR_5g088810 | 84.444 | 45 | 7 | 0 | 132 | 176 | 21 | 65 | 1.40e-17 | 73.9 |
| MsG0380013937.01.T01 | MTR_8g023445 | 34.459 | 148 | 80 | 2 | 60 | 191 | 1847 | 1993 | 1.41e-17 | 81.3 |
| MsG0380013937.01.T01 | MTR_1g023500 | 26.339 | 224 | 135 | 4 | 1 | 209 | 985 | 1193 | 7.37e-17 | 79.0 |
| MsG0380013937.01.T01 | MTR_4g124030 | 27.841 | 176 | 121 | 1 | 1 | 176 | 957 | 1126 | 3.74e-13 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013937.01.T01 | ATCG00190 | 93.333 | 210 | 14 | 0 | 1 | 210 | 863 | 1072 | 7.53e-138 | 412 |
| MsG0380013937.01.T01 | AT4G21710 | 28.155 | 206 | 118 | 4 | 1 | 191 | 982 | 1172 | 6.48e-19 | 85.1 |
| MsG0380013937.01.T01 | AT5G45140 | 28.431 | 204 | 121 | 2 | 1 | 189 | 957 | 1150 | 1.71e-17 | 80.9 |
| MsG0380013937.01.T01 | AT5G45140 | 28.431 | 204 | 121 | 2 | 1 | 189 | 957 | 1150 | 1.71e-17 | 80.9 |
| MsG0380013937.01.T01 | AT1G29940 | 28.025 | 157 | 102 | 2 | 1 | 152 | 941 | 1091 | 3.79e-12 | 65.1 |
Find 56 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGTTTCGCCCTTCCTTTA+AGG | 0.204769 | 3:-48464407 | None:intergenic |
| GTTACACAACAACCCCTTAA+AGG | 0.212213 | 3:+48464393 | MsG0380013937.01.T01:CDS |
| TGGATTCGATGTTTGTTTAC+TGG | 0.222331 | 3:-48464208 | None:intergenic |
| TTTGAACAGCCTGTTATAAT+AGG | 0.245428 | 3:+48464294 | MsG0380013937.01.T01:CDS |
| TGAATTAATTTCAAGATATA+AGG | 0.248171 | 3:-48464321 | None:intergenic |
| TTGTTTCGCCCTTCCTTTAA+GGG | 0.292882 | 3:-48464406 | None:intergenic |
| TCAAATATTCCGCTCTTTCC+CGG | 0.314064 | 3:-48464252 | None:intergenic |
| GGAATATTTGATGGAAGAAC+AGG | 0.334674 | 3:+48464264 | MsG0380013937.01.T01:CDS |
| AATGAATGTAGGACAGATAT+TGG | 0.340082 | 3:+48464068 | None:intergenic |
| AAACTTCCTTCCTTTGAATC+TGG | 0.340714 | 3:-48464677 | None:intergenic |
| TCTGGAGCATCCACAGGTTT+AGG | 0.340865 | 3:-48464576 | None:intergenic |
| ACGAGCAATCGAAAAGATTC+TGG | 0.342745 | 3:-48464594 | None:intergenic |
| GTACTCGGAACTACGATTAT+TGG | 0.356639 | 3:+48464543 | MsG0380013937.01.T01:CDS |
| CGTTCGAGAACTACGATCTT+TGG | 0.364953 | 3:+48464614 | MsG0380013937.01.T01:CDS |
| TCCGCTCTTTCCCGGATACT+CGG | 0.379405 | 3:-48464244 | None:intergenic |
| GAATGTTCACTCGGATTAGC+TGG | 0.386652 | 3:+48464090 | MsG0380013937.01.T01:CDS |
| GATATTTGAACCCGAGTATC+CGG | 0.389575 | 3:+48464233 | MsG0380013937.01.T01:CDS |
| ATGGAGGTTTGGGCGCTCGA+GGG | 0.426714 | 3:+48464450 | MsG0380013937.01.T01:CDS |
| ATTATAACAGGCTGTTCAAA+AGG | 0.436541 | 3:-48464291 | None:intergenic |
| AGGACAACGGGTAGGCGAAA+TGG | 0.436703 | 3:+48464431 | MsG0380013937.01.T01:CDS |
| AATGGAGGTTTGGGCGCTCG+AGG | 0.461267 | 3:+48464449 | MsG0380013937.01.T01:CDS |
| GGTAGGCGAAATGGAGGTTT+GGG | 0.465784 | 3:+48464440 | MsG0380013937.01.T01:CDS |
| GGGTAGGCGAAATGGAGGTT+TGG | 0.465926 | 3:+48464439 | MsG0380013937.01.T01:CDS |
| GGAAAGAGCGGAATATTTGA+TGG | 0.469164 | 3:+48464255 | MsG0380013937.01.T01:CDS |
| CCGCTCTTTCCCGGATACTC+GGG | 0.478316 | 3:-48464243 | None:intergenic |
| GGCGAAACAAGGAGGACAAC+GGG | 0.481846 | 3:+48464419 | MsG0380013937.01.T01:CDS |
| TGTTCATATCTCTCATCAAA+AGG | 0.491842 | 3:-48464144 | None:intergenic |
| CAAGTTGATGATAAAATACA+TGG | 0.502523 | 3:+48464345 | MsG0380013937.01.T01:CDS |
| TGGAGGTTTGGGCGCTCGAG+GGG | 0.505858 | 3:+48464451 | MsG0380013937.01.T01:CDS |
| AGAACTACGATCTTTGGCTC+TGG | 0.513829 | 3:+48464620 | MsG0380013937.01.T01:CDS |
| GTAAACAAACATCGAATCCA+TGG | 0.519391 | 3:+48464211 | MsG0380013937.01.T01:CDS |
| AAAGATTCTGGAGCATCCAC+AGG | 0.531781 | 3:-48464582 | None:intergenic |
| AGAAAGACTTCCAGATTCAA+AGG | 0.533242 | 3:+48464667 | MsG0380013937.01.T01:CDS |
| CACAACAACCCCTTAAAGGA+AGG | 0.535519 | 3:+48464397 | MsG0380013937.01.T01:CDS |
| TACTCGGGTTCAAATATCCA+TGG | 0.546454 | 3:-48464228 | None:intergenic |
| CAGATATTGGAATGTTCACT+CGG | 0.551609 | 3:+48464081 | MsG0380013937.01.T01:CDS |
| CTCGGAACTACGATTATTGG+AGG | 0.559077 | 3:+48464546 | MsG0380013937.01.T01:CDS |
| AGACTTCCAGATTCAAAGGA+AGG | 0.563377 | 3:+48464671 | MsG0380013937.01.T01:CDS |
| GGAAGTCTTTCTCAGATACA+AGG | 0.565963 | 3:-48464656 | None:intergenic |
| AACAAGTGCATAATGTCCAC+TGG | 0.566338 | 3:-48464373 | None:intergenic |
| TGTTTCGCCCTTCCTTTAAG+GGG | 0.567625 | 3:-48464405 | None:intergenic |
| AATGTTCACTCGGATTAGCT+GGG | 0.569063 | 3:+48464091 | MsG0380013937.01.T01:CDS |
| CTTAAAGGAAGGGCGAAACA+AGG | 0.579473 | 3:+48464408 | MsG0380013937.01.T01:CDS |
| ATATTTGAACCCGAGTATCC+GGG | 0.581389 | 3:+48464234 | MsG0380013937.01.T01:CDS |
| CCCGAGTATCCGGGAAAGAG+CGG | 0.584519 | 3:+48464243 | MsG0380013937.01.T01:CDS |
| GGGCGAAACAAGGAGGACAA+CGG | 0.598993 | 3:+48464418 | MsG0380013937.01.T01:CDS |
| AAAATACATGGACGTTCCAG+TGG | 0.614997 | 3:+48464357 | MsG0380013937.01.T01:CDS |
| TGATGAGAGATATGAACAGG+AGG | 0.620223 | 3:+48464149 | MsG0380013937.01.T01:CDS |
| GTACCCTCACGAATGAATGT+AGG | 0.622385 | 3:+48464057 | None:intergenic |
| AAACAAGGAGGACAACGGGT+AGG | 0.626804 | 3:+48464423 | MsG0380013937.01.T01:CDS |
| ACAACAACCCCTTAAAGGAA+GGG | 0.636584 | 3:+48464398 | MsG0380013937.01.T01:CDS |
| AGGAACAATACCTAAACCTG+TGG | 0.656849 | 3:+48464566 | MsG0380013937.01.T01:CDS |
| AAAGCTCGTCAAGAAGTACT+CGG | 0.659322 | 3:+48464528 | MsG0380013937.01.T01:CDS |
| ACAACGGGTAGGCGAAATGG+AGG | 0.659407 | 3:+48464434 | MsG0380013937.01.T01:CDS |
| GAATATTTGATGGAAGAACA+GGG | 0.689025 | 3:+48464265 | MsG0380013937.01.T01:CDS |
| AAAGGAAGGGCGAAACAAGG+AGG | 0.708508 | 3:+48464411 | MsG0380013937.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTAATTTCAAGATATA+AGG | - | Chr3:48464324-48464343 | None:intergenic | 15.0% |
| !! | TAAGGTTTTCCTATTATAAC+AGG | - | Chr3:48464306-48464325 | None:intergenic | 25.0% |
| !!! | CAAGTTGATGATAAAATACA+TGG | + | Chr3:48464345-48464364 | MsG0380013937.01.T01:CDS | 25.0% |
| ATTATAACAGGCTGTTCAAA+AGG | - | Chr3:48464294-48464313 | None:intergenic | 30.0% | |
| GAATATTTGATGGAAGAACA+GGG | + | Chr3:48464265-48464284 | MsG0380013937.01.T01:CDS | 30.0% | |
| TGTTCATATCTCTCATCAAA+AGG | - | Chr3:48464147-48464166 | None:intergenic | 30.0% | |
| TTTGAACAGCCTGTTATAAT+AGG | + | Chr3:48464294-48464313 | MsG0380013937.01.T01:CDS | 30.0% | |
| ! | TTCAGAAAACACAAGTTTTC+TGG | - | Chr3:48464178-48464197 | None:intergenic | 30.0% |
| ! | TTTTGATGAGAGATATGAAC+AGG | + | Chr3:48464146-48464165 | MsG0380013937.01.T01:CDS | 30.0% |
| AAACTTCCTTCCTTTGAATC+TGG | - | Chr3:48464680-48464699 | None:intergenic | 35.0% | |
| AGAAAGACTTCCAGATTCAA+AGG | + | Chr3:48464667-48464686 | MsG0380013937.01.T01:CDS | 35.0% | |
| CAGATATTGGAATGTTCACT+CGG | + | Chr3:48464081-48464100 | MsG0380013937.01.T01:CDS | 35.0% | |
| GGAATATTTGATGGAAGAAC+AGG | + | Chr3:48464264-48464283 | MsG0380013937.01.T01:CDS | 35.0% | |
| GTAAACAAACATCGAATCCA+TGG | + | Chr3:48464211-48464230 | MsG0380013937.01.T01:CDS | 35.0% | |
| TGGATTCGATGTTTGTTTAC+TGG | - | Chr3:48464211-48464230 | None:intergenic | 35.0% | |
| AAAATACATGGACGTTCCAG+TGG | + | Chr3:48464357-48464376 | MsG0380013937.01.T01:CDS | 40.0% | |
| AACAAGTGCATAATGTCCAC+TGG | - | Chr3:48464376-48464395 | None:intergenic | 40.0% | |
| ACAACAACCCCTTAAAGGAA+GGG | + | Chr3:48464398-48464417 | MsG0380013937.01.T01:CDS | 40.0% | |
| ACGAGCAATCGAAAAGATTC+TGG | - | Chr3:48464597-48464616 | None:intergenic | 40.0% | |
| AGACTTCCAGATTCAAAGGA+AGG | + | Chr3:48464671-48464690 | MsG0380013937.01.T01:CDS | 40.0% | |
| AGGAACAATACCTAAACCTG+TGG | + | Chr3:48464566-48464585 | MsG0380013937.01.T01:CDS | 40.0% | |
| ATATTTGAACCCGAGTATCC+GGG | + | Chr3:48464234-48464253 | MsG0380013937.01.T01:CDS | 40.0% | |
| GATATTTGAACCCGAGTATC+CGG | + | Chr3:48464233-48464252 | MsG0380013937.01.T01:CDS | 40.0% | |
| GGAAAGAGCGGAATATTTGA+TGG | + | Chr3:48464255-48464274 | MsG0380013937.01.T01:CDS | 40.0% | |
| GGAAGTCTTTCTCAGATACA+AGG | - | Chr3:48464659-48464678 | None:intergenic | 40.0% | |
| GTACTCGGAACTACGATTAT+TGG | + | Chr3:48464543-48464562 | MsG0380013937.01.T01:CDS | 40.0% | |
| GTTACACAACAACCCCTTAA+AGG | + | Chr3:48464393-48464412 | MsG0380013937.01.T01:CDS | 40.0% | |
| TACTCGGGTTCAAATATCCA+TGG | - | Chr3:48464231-48464250 | None:intergenic | 40.0% | |
| TCAAATATTCCGCTCTTTCC+CGG | - | Chr3:48464255-48464274 | None:intergenic | 40.0% | |
| TGATGAGAGATATGAACAGG+AGG | + | Chr3:48464149-48464168 | MsG0380013937.01.T01:CDS | 40.0% | |
| TTGTTTCGCCCTTCCTTTAA+GGG | - | Chr3:48464409-48464428 | None:intergenic | 40.0% | |
| !! | AAAGCTCGTCAAGAAGTACT+CGG | + | Chr3:48464528-48464547 | MsG0380013937.01.T01:CDS | 40.0% |
| !! | AATGTTCACTCGGATTAGCT+GGG | + | Chr3:48464091-48464110 | MsG0380013937.01.T01:CDS | 40.0% |
| CACAACAACCCCTTAAAGGA+AGG | + | Chr3:48464397-48464416 | MsG0380013937.01.T01:CDS | 45.0% | |
| CGTTCGAGAACTACGATCTT+TGG | + | Chr3:48464614-48464633 | MsG0380013937.01.T01:CDS | 45.0% | |
| CTCGGAACTACGATTATTGG+AGG | + | Chr3:48464546-48464565 | MsG0380013937.01.T01:CDS | 45.0% | |
| CTTAAAGGAAGGGCGAAACA+AGG | + | Chr3:48464408-48464427 | MsG0380013937.01.T01:CDS | 45.0% | |
| CTTGTTTCGCCCTTCCTTTA+AGG | - | Chr3:48464410-48464429 | None:intergenic | 45.0% | |
| TGTTTCGCCCTTCCTTTAAG+GGG | - | Chr3:48464408-48464427 | None:intergenic | 45.0% | |
| ! | AGAACTACGATCTTTGGCTC+TGG | + | Chr3:48464620-48464639 | MsG0380013937.01.T01:CDS | 45.0% |
| !! | AAAGATTCTGGAGCATCCAC+AGG | - | Chr3:48464585-48464604 | None:intergenic | 45.0% |
| !! | GAATGTTCACTCGGATTAGC+TGG | + | Chr3:48464090-48464109 | MsG0380013937.01.T01:CDS | 45.0% |
| AAACAAGGAGGACAACGGGT+AGG | + | Chr3:48464423-48464442 | MsG0380013937.01.T01:CDS | 50.0% | |
| AAAGGAAGGGCGAAACAAGG+AGG | + | Chr3:48464411-48464430 | MsG0380013937.01.T01:CDS | 50.0% | |
| GGTAGGCGAAATGGAGGTTT+GGG | + | Chr3:48464440-48464459 | MsG0380013937.01.T01:CDS | 50.0% | |
| ! | TCTGGAGCATCCACAGGTTT+AGG | - | Chr3:48464579-48464598 | None:intergenic | 50.0% |
| ACAACGGGTAGGCGAAATGG+AGG | + | Chr3:48464434-48464453 | MsG0380013937.01.T01:CDS | 55.0% | |
| AGGACAACGGGTAGGCGAAA+TGG | + | Chr3:48464431-48464450 | MsG0380013937.01.T01:CDS | 55.0% | |
| GGCGAAACAAGGAGGACAAC+GGG | + | Chr3:48464419-48464438 | MsG0380013937.01.T01:CDS | 55.0% | |
| GGGCGAAACAAGGAGGACAA+CGG | + | Chr3:48464418-48464437 | MsG0380013937.01.T01:CDS | 55.0% | |
| GGGTAGGCGAAATGGAGGTT+TGG | + | Chr3:48464439-48464458 | MsG0380013937.01.T01:CDS | 55.0% | |
| TCCGCTCTTTCCCGGATACT+CGG | - | Chr3:48464247-48464266 | None:intergenic | 55.0% | |
| AATGGAGGTTTGGGCGCTCG+AGG | + | Chr3:48464449-48464468 | MsG0380013937.01.T01:CDS | 60.0% | |
| ATGGAGGTTTGGGCGCTCGA+GGG | + | Chr3:48464450-48464469 | MsG0380013937.01.T01:CDS | 60.0% | |
| CCCGAGTATCCGGGAAAGAG+CGG | + | Chr3:48464243-48464262 | MsG0380013937.01.T01:CDS | 60.0% | |
| CCGCTCTTTCCCGGATACTC+GGG | - | Chr3:48464246-48464265 | None:intergenic | 60.0% | |
| TGGAGGTTTGGGCGCTCGAG+GGG | + | Chr3:48464451-48464470 | MsG0380013937.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48464069 | 48464701 | 48464069 | ID=MsG0380013937.01;Name=MsG0380013937.01 |
| Chr3 | mRNA | 48464069 | 48464701 | 48464069 | ID=MsG0380013937.01.T01;Parent=MsG0380013937.01;Name=MsG0380013937.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|210 |
| Chr3 | exon | 48464069 | 48464701 | 48464069 | ID=MsG0380013937.01.T01:exon:8504;Parent=MsG0380013937.01.T01 |
| Chr3 | CDS | 48464069 | 48464701 | 48464069 | ID=MsG0380013937.01.T01:cds;Parent=MsG0380013937.01.T01 |
| Gene Sequence |
| Protein sequence |
