AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013962.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013962.01.T01 MTR_4g051120 99.512 205 1 0 1 205 1 205 1.23e-140 426
MsG0380013962.01.T01 MTR_8g016520 96.809 188 6 0 1 188 1 188 1.14e-133 375
MsG0380013962.01.T01 MTR_0002s0510 92.118 203 16 0 3 205 1670 1872 3.24e-126 393
MsG0380013962.01.T01 MTR_2g080240 96.000 75 3 0 1 75 20 94 3.19e-47 151
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013962.01.T01 ATCG00170 96.517 201 7 0 5 205 1 201 4.88e-133 405

Find 51 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACAACAAAGTTCTATTT+TGG 0.177929 3:-48522065 MsG0380013962.01.T01:CDS
TCTGCTAATAAGTCGTTTAA+TGG 0.223864 3:+48522237 None:intergenic
ATCCTCTCATTCCGACTAAT+TGG 0.248879 3:+48521858 None:intergenic
CATTATATGGACTGGATTAA+AGG 0.289410 3:+48521919 None:intergenic
GGGTCAGTCATCCTAAAATT+AGG 0.301712 3:+48521941 None:intergenic
ATACCGGATTAATAGTATTC+TGG 0.304788 3:-48521690 MsG0380013962.01.T01:CDS
TTCTCGTAAATTGCTTTGAA+TGG 0.322270 3:+48521799 None:intergenic
CAGTATCCACAACCCCTTTA+CGG 0.329838 3:+48521735 None:intergenic
AACTTTGTTGTTCAGCATCT+TGG 0.334259 3:+48522074 None:intergenic
TACGCCAATCCATTGAAATA+TGG 0.339395 3:-48522000 MsG0380013962.01.T01:CDS
ACTGCTACATCTATTTCATT+AGG 0.354727 3:-48522139 MsG0380013962.01.T01:CDS
ATTATATGGACTGGATTAAA+GGG 0.370361 3:+48521920 None:intergenic
CGAAATGAGCGACTTCAAAT+AGG 0.374640 3:+48521653 None:intergenic
TGGACTAGCCATCCTTTAGA+AGG 0.382153 3:+48522094 None:intergenic
TTCAAATAGGTTCATTGCTC+CGG 0.387556 3:+48521666 None:intergenic
GTGGAATATGGAGGTACTTA+TGG 0.396890 3:-48522308 None:intergenic
AGTATCCACAACCCCTTTAC+GGG 0.401249 3:+48521736 None:intergenic
CTTGCTACGGAGCCCGTAAA+GGG 0.410011 3:-48521748 MsG0380013962.01.T01:CDS
AGCAGATTAATAGACCATTT+CGG 0.411046 3:-48522220 MsG0380013962.01.T01:CDS
TCTCGTAAATTGCTTTGAAT+GGG 0.433790 3:+48521800 None:intergenic
CATACCATATTTCAATGGAT+TGG 0.443039 3:+48521996 None:intergenic
GGAGGTACTTATGGCAGAAC+GGG 0.448573 3:-48522299 MsG0380013962.01.T01:CDS
TGTAGCATACCATATTTCAA+TGG 0.450731 3:+48521991 None:intergenic
TACCGGATTAATAGTATTCT+GGG 0.455516 3:-48521689 MsG0380013962.01.T01:CDS
CTGGATCAAGTAAAGACTCT+AGG 0.462436 3:-48522175 MsG0380013962.01.T01:CDS
CTAGAGTCTTTACTTGATCC+AGG 0.463607 3:+48522176 None:intergenic
GTCTTTCACAATAACGTGAT+AGG 0.489141 3:-48522268 MsG0380013962.01.T01:CDS
GTTATTGTGAAAGACTAGAT+TGG 0.489846 3:+48522276 None:intergenic
TCTTGCTACGGAGCCCGTAA+AGG 0.490200 3:-48521749 MsG0380013962.01.T01:CDS
GGCATATACATCACACATCC+TGG 0.495134 3:-48522194 MsG0380013962.01.T01:CDS
TAACAATACCTTCTAAAGGA+TGG 0.495965 3:-48522102 MsG0380013962.01.T01:CDS
GGCCCAGAATACTATTAATC+CGG 0.497844 3:+48521687 None:intergenic
CGGAGCCCGTAAAGGGGTTG+TGG 0.513039 3:-48521741 MsG0380013962.01.T01:CDS
AAATCAATCATTTGTCCTTG+TGG 0.513235 3:+48521824 None:intergenic
TAGATGTAGCAGTAGCTTGA+CGG 0.516966 3:+48522149 None:intergenic
ATGTATATGCCATTCCGAAA+TGG 0.525535 3:+48522206 None:intergenic
GAATATATAATTTCTTGCTA+CGG 0.534554 3:-48521761 MsG0380013962.01.T01:CDS
TGGATCAAGTAAAGACTCTA+GGG 0.538268 3:-48522174 MsG0380013962.01.T01:CDS
GAACATCAGATGCTGGATAC+CGG 0.556559 3:-48521706 MsG0380013962.01.T01:CDS
GCTGTACGAACATCAGATGC+TGG 0.573261 3:-48521713 MsG0380013962.01.T01:CDS
CACCAATTAGTCGGAATGAG+AGG 0.580106 3:-48521860 MsG0380013962.01.T01:CDS
ATTAATAGACCATTTCGGAA+TGG 0.587547 3:-48522215 MsG0380013962.01.T01:CDS
ATTCAAAGCAATTTACGAGA+AGG 0.601229 3:-48521797 MsG0380013962.01.T01:CDS
TGGAGGTACTTATGGCAGAA+CGG 0.603183 3:-48522300 MsG0380013962.01.T01:CDS
GGATTAATAGTATTCTGGGC+CGG 0.606095 3:-48521685 MsG0380013962.01.T01:CDS
TCTCAAGTGCACCAATTAGT+CGG 0.615181 3:-48521869 MsG0380013962.01.T01:CDS
TTGCTACGGAGCCCGTAAAG+GGG 0.638134 3:-48521747 MsG0380013962.01.T01:CDS
TTTCACAATAACGTGATAGG+TGG 0.645396 3:-48522265 MsG0380013962.01.T01:CDS
TTATATGGACTGGATTAAAG+GGG 0.684921 3:+48521921 None:intergenic
TCAGTATGGGAATGTACATG+CGG 0.691247 3:-48522032 MsG0380013962.01.T01:CDS
GGATTAATGTCAGATCCACA+AGG 0.711466 3:-48521839 MsG0380013962.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATATATAATTTCTTGCTA+CGG - Chr3:48522207-48522226 MsG0380013962.01.T01:CDS 20.0%
!!! AAGAAATGAATCCTAATTTT+AGG - Chr3:48522016-48522035 MsG0380013962.01.T01:CDS 20.0%
! TGGAAAAACATAATCAGTAT+GGG - Chr3:48521923-48521942 MsG0380013962.01.T01:CDS 25.0%
! TTGGAAAAACATAATCAGTA+TGG - Chr3:48521922-48521941 MsG0380013962.01.T01:CDS 25.0%
!! CTTTTAACAATACCTTCTAA+AGG - Chr3:48521862-48521881 MsG0380013962.01.T01:CDS 25.0%
!! TGAACAACAAAGTTCTATTT+TGG - Chr3:48521903-48521922 MsG0380013962.01.T01:CDS 25.0%
!!! ATTATATGGACTGGATTAAA+GGG + Chr3:48522051-48522070 None:intergenic 25.0%
AAATCAATCATTTGTCCTTG+TGG + Chr3:48522147-48522166 None:intergenic 30.0%
ACTGCTACATCTATTTCATT+AGG - Chr3:48521829-48521848 MsG0380013962.01.T01:CDS 30.0%
AGCAGATTAATAGACCATTT+CGG - Chr3:48521748-48521767 MsG0380013962.01.T01:CDS 30.0%
ATACCGGATTAATAGTATTC+TGG - Chr3:48522278-48522297 MsG0380013962.01.T01:CDS 30.0%
ATTAATAGACCATTTCGGAA+TGG - Chr3:48521753-48521772 MsG0380013962.01.T01:CDS 30.0%
ATTCAAAGCAATTTACGAGA+AGG - Chr3:48522171-48522190 MsG0380013962.01.T01:CDS 30.0%
CATACCATATTTCAATGGAT+TGG + Chr3:48521975-48521994 None:intergenic 30.0%
CTCCTGAAAAAGACATTATA+TGG + Chr3:48522065-48522084 None:intergenic 30.0%
GAAAAAGACATTATATGGAC+TGG + Chr3:48522060-48522079 None:intergenic 30.0%
GTTATTGTGAAAGACTAGAT+TGG + Chr3:48521695-48521714 None:intergenic 30.0%
TACCGGATTAATAGTATTCT+GGG - Chr3:48522279-48522298 MsG0380013962.01.T01:CDS 30.0%
TCTGCTAATAAGTCGTTTAA+TGG + Chr3:48521734-48521753 None:intergenic 30.0%
TGTAGCATACCATATTTCAA+TGG + Chr3:48521980-48521999 None:intergenic 30.0%
! GTCCATATAATGTCTTTTTC+AGG - Chr3:48522060-48522079 MsG0380013962.01.T01:CDS 30.0%
! TAACAATACCTTCTAAAGGA+TGG - Chr3:48521866-48521885 MsG0380013962.01.T01:CDS 30.0%
! TCTCGTAAATTGCTTTGAAT+GGG + Chr3:48522171-48522190 None:intergenic 30.0%
! TTCTCGTAAATTGCTTTGAA+TGG + Chr3:48522172-48522191 None:intergenic 30.0%
!! CATTATATGGACTGGATTAA+AGG + Chr3:48522052-48522071 None:intergenic 30.0%
!! TTATATGGACTGGATTAAAG+GGG + Chr3:48522050-48522069 None:intergenic 30.0%
ATGTATATGCCATTCCGAAA+TGG + Chr3:48521765-48521784 None:intergenic 35.0%
GTCTTTCACAATAACGTGAT+AGG - Chr3:48521700-48521719 MsG0380013962.01.T01:CDS 35.0%
TACGCCAATCCATTGAAATA+TGG - Chr3:48521968-48521987 MsG0380013962.01.T01:CDS 35.0%
TGGATCAAGTAAAGACTCTA+GGG - Chr3:48521794-48521813 MsG0380013962.01.T01:CDS 35.0%
TTTCACAATAACGTGATAGG+TGG - Chr3:48521703-48521722 MsG0380013962.01.T01:CDS 35.0%
!! AACTTTGTTGTTCAGCATCT+TGG + Chr3:48521897-48521916 None:intergenic 35.0%
ATCCTCTCATTCCGACTAAT+TGG + Chr3:48522113-48522132 None:intergenic 40.0%
CTAGAGTCTTTACTTGATCC+AGG + Chr3:48521795-48521814 None:intergenic 40.0%
CTGGATCAAGTAAAGACTCT+AGG - Chr3:48521793-48521812 MsG0380013962.01.T01:CDS 40.0%
GGATTAATAGTATTCTGGGC+CGG - Chr3:48522283-48522302 MsG0380013962.01.T01:CDS 40.0%
GGATTAATGTCAGATCCACA+AGG - Chr3:48522129-48522148 MsG0380013962.01.T01:CDS 40.0%
GGCCCAGAATACTATTAATC+CGG + Chr3:48522284-48522303 None:intergenic 40.0%
GGGTCAGTCATCCTAAAATT+AGG + Chr3:48522030-48522049 None:intergenic 40.0%
TAGATGTAGCAGTAGCTTGA+CGG + Chr3:48521822-48521841 None:intergenic 40.0%
TCAGTATGGGAATGTACATG+CGG - Chr3:48521936-48521955 MsG0380013962.01.T01:CDS 40.0%
TCTCAAGTGCACCAATTAGT+CGG - Chr3:48522099-48522118 MsG0380013962.01.T01:CDS 40.0%
!!! ATGTCTTTTTCAGGAGCTAG+AGG - Chr3:48522069-48522088 MsG0380013962.01.T01:CDS 40.0%
AGTATCCACAACCCCTTTAC+GGG + Chr3:48522235-48522254 None:intergenic 45.0%
CACCAATTAGTCGGAATGAG+AGG - Chr3:48522108-48522127 MsG0380013962.01.T01:CDS 45.0%
CAGTATCCACAACCCCTTTA+CGG + Chr3:48522236-48522255 None:intergenic 45.0%
GAACATCAGATGCTGGATAC+CGG - Chr3:48522262-48522281 MsG0380013962.01.T01:CDS 45.0%
GGCATATACATCACACATCC+TGG - Chr3:48521774-48521793 MsG0380013962.01.T01:CDS 45.0%
!! TGGACTAGCCATCCTTTAGA+AGG + Chr3:48521877-48521896 None:intergenic 45.0%
!! TGGAGGTACTTATGGCAGAA+CGG - Chr3:48521668-48521687 MsG0380013962.01.T01:CDS 45.0%
GCTGTACGAACATCAGATGC+TGG - Chr3:48522255-48522274 MsG0380013962.01.T01:CDS 50.0%
!! GGAGGTACTTATGGCAGAAC+GGG - Chr3:48521669-48521688 MsG0380013962.01.T01:CDS 50.0%
CTTGCTACGGAGCCCGTAAA+GGG - Chr3:48522220-48522239 MsG0380013962.01.T01:CDS 55.0%
TCTTGCTACGGAGCCCGTAA+AGG - Chr3:48522219-48522238 MsG0380013962.01.T01:CDS 55.0%
TTGCTACGGAGCCCGTAAAG+GGG - Chr3:48522221-48522240 MsG0380013962.01.T01:CDS 55.0%
CGGAGCCCGTAAAGGGGTTG+TGG - Chr3:48522227-48522246 MsG0380013962.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48521667 48522323 48521667 ID=MsG0380013962.01;Name=MsG0380013962.01
Chr3 mRNA 48521667 48522323 48521667 ID=MsG0380013962.01.T01;Parent=MsG0380013962.01;Name=MsG0380013962.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|218
Chr3 exon 48521667 48522323 48521667 ID=MsG0380013962.01.T01:exon:9208;Parent=MsG0380013962.01.T01
Chr3 CDS 48521667 48522323 48521667 ID=MsG0380013962.01.T01:cds;Parent=MsG0380013962.01.T01
Gene Sequence

>MsG0380013962.01.T01

ATGGAGGTACTTATGGCAGAACGGGCCAATCTAGTCTTTCACAATAACGTGATAGGTGGAACTGCCATTAAACGACTTATTAGCAGATTAATAGACCATTTCGGAATGGCATATACATCACACATCCTGGATCAAGTAAAGACTCTAGGGTTCCGTCAAGCTACTGCTACATCTATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAAGGATGGCTAGTCCAAGATGCTGAACAACAAAGTTCTATTTTGGAAAAACATAATCAGTATGGGAATGTACATGCGGTAGAAAAATTACGCCAATCCATTGAAATATGGTATGCTACAAGCGAATATTTGCGACAAGAAATGAATCCTAATTTTAGGATGACTGACCCCTTTAATCCAGTCCATATAATGTCTTTTTCAGGAGCTAGAGGAAATGCATCTCAAGTGCACCAATTAGTCGGAATGAGAGGATTAATGTCAGATCCACAAGGACAAATGATTGATTTACCCATTCAAAGCAATTTACGAGAAGGACTGTCTTTAACAGAATATATAATTTCTTGCTACGGAGCCCGTAAAGGGGTTGTGGATACTGCTGTACGAACATCAGATGCTGGATACCGGATTAATAGTATTCTGGGCCGGAGCAATGAACCTATTTGA

Protein sequence

>MsG0380013962.01.T01

MEVLMAERANLVFHNNVIGGTAIKRLISRLIDHFGMAYTSHILDQVKTLGFRQATATSISLGIDDLLTIPSKGWLVQDAEQQSSILEKHNQYGNVHAVEKLRQSIEIWYATSEYLRQEMNPNFRMTDPFNPVHIMSFSGARGNASQVHQLVGMRGLMSDPQGQMIDLPIQSNLREGLSLTEYIISCYGARKGVVDTAVRTSDAGYRINSILGRSNEPI*