Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013962.01.T01 | RDY14679.1 | 97.561 | 205 | 5 | 0 | 1 | 205 | 1 | 205 | 6.60E-143 | 415 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013962.01.T01 | A0A371II30 | 97.561 | 205 | 5 | 0 | 1 | 205 | 1 | 205 | 3.15e-143 | 415 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047967.01 | MsG0380013962.01 | 0.807782 | 4.272442e-50 | 2.401071e-47 |
| MsG0080048560.01 | MsG0380013962.01 | 0.836154 | 1.104967e-56 | 1.380722e-53 |
| MsG0080048690.01 | MsG0380013962.01 | 0.843344 | 1.484336e-58 | 2.315819e-55 |
| MsG0080048691.01 | MsG0380013962.01 | 0.844564 | 6.993520e-59 | 1.133627e-55 |
| MsG0080048692.01 | MsG0380013962.01 | 0.873418 | 1.493226e-67 | 6.476107e-64 |
| MsG0080048693.01 | MsG0380013962.01 | 0.859835 | 3.142076e-63 | 8.413333e-60 |
| MsG0080048694.01 | MsG0380013962.01 | 0.822491 | 2.301963e-53 | 1.929697e-50 |
| MsG0080048695.01 | MsG0380013962.01 | 0.822106 | 2.828724e-53 | 2.345216e-50 |
| MsG0080048697.01 | MsG0380013962.01 | 0.847882 | 8.729752e-60 | 1.573734e-56 |
| MsG0080048698.01 | MsG0380013962.01 | 0.867023 | 1.857249e-65 | 6.388751e-62 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013962.01.T01 | MTR_4g051120 | 99.512 | 205 | 1 | 0 | 1 | 205 | 1 | 205 | 1.23e-140 | 426 |
| MsG0380013962.01.T01 | MTR_8g016520 | 96.809 | 188 | 6 | 0 | 1 | 188 | 1 | 188 | 1.14e-133 | 375 |
| MsG0380013962.01.T01 | MTR_0002s0510 | 92.118 | 203 | 16 | 0 | 3 | 205 | 1670 | 1872 | 3.24e-126 | 393 |
| MsG0380013962.01.T01 | MTR_2g080240 | 96.000 | 75 | 3 | 0 | 1 | 75 | 20 | 94 | 3.19e-47 | 151 |
Find 51 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCAATCATTTGTCCTTG+TGG | 0.513235 | 3:+48521824 | None:intergenic |
| AACTTTGTTGTTCAGCATCT+TGG | 0.334259 | 3:+48522074 | None:intergenic |
| ACTGCTACATCTATTTCATT+AGG | 0.354727 | 3:-48522139 | MsG0380013962.01.T01:CDS |
| AGCAGATTAATAGACCATTT+CGG | 0.411046 | 3:-48522220 | MsG0380013962.01.T01:CDS |
| AGTATCCACAACCCCTTTAC+GGG | 0.401249 | 3:+48521736 | None:intergenic |
| ATACCGGATTAATAGTATTC+TGG | 0.304788 | 3:-48521690 | MsG0380013962.01.T01:CDS |
| ATCCTCTCATTCCGACTAAT+TGG | 0.248879 | 3:+48521858 | None:intergenic |
| ATGTATATGCCATTCCGAAA+TGG | 0.525535 | 3:+48522206 | None:intergenic |
| ATTAATAGACCATTTCGGAA+TGG | 0.587547 | 3:-48522215 | MsG0380013962.01.T01:CDS |
| ATTATATGGACTGGATTAAA+GGG | 0.370361 | 3:+48521920 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCAATCATTTGTCCTTG+TGG | + | Chr3:48522147-48522166 | None:intergenic | 30.0% | |
| ACTGCTACATCTATTTCATT+AGG | - | Chr3:48521829-48521848 | MsG0380013962.01.T01:CDS | 30.0% | |
| AGCAGATTAATAGACCATTT+CGG | - | Chr3:48521748-48521767 | MsG0380013962.01.T01:CDS | 30.0% | |
| ATACCGGATTAATAGTATTC+TGG | - | Chr3:48522278-48522297 | MsG0380013962.01.T01:CDS | 30.0% | |
| ATTAATAGACCATTTCGGAA+TGG | - | Chr3:48521753-48521772 | MsG0380013962.01.T01:CDS | 30.0% | |
| ATTCAAAGCAATTTACGAGA+AGG | - | Chr3:48522171-48522190 | MsG0380013962.01.T01:CDS | 30.0% | |
| CATACCATATTTCAATGGAT+TGG | + | Chr3:48521975-48521994 | None:intergenic | 30.0% | |
| CTCCTGAAAAAGACATTATA+TGG | + | Chr3:48522065-48522084 | None:intergenic | 30.0% | |
| GAAAAAGACATTATATGGAC+TGG | + | Chr3:48522060-48522079 | None:intergenic | 30.0% | |
| GTTATTGTGAAAGACTAGAT+TGG | + | Chr3:48521695-48521714 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48521667 | 48522323 | 48521667 | ID=MsG0380013962.01;Name=MsG0380013962.01 |
| Chr3 | mRNA | 48521667 | 48522323 | 48521667 | ID=MsG0380013962.01.T01;Parent=MsG0380013962.01;Name=MsG0380013962.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|218 |
| Chr3 | exon | 48521667 | 48522323 | 48521667 | ID=MsG0380013962.01.T01:exon:9208;Parent=MsG0380013962.01.T01 |
| Chr3 | CDS | 48521667 | 48522323 | 48521667 | ID=MsG0380013962.01.T01:cds;Parent=MsG0380013962.01.T01 |
| Gene Sequence |
>MsG0380013962.01.T01 ATGGAGGTACTTATGGCAGAACGGGCCAATCTAGTCTTTCACAATAACGTGATAGGTGGAACTGCCATTAAACGACTTATTAGCAGATTAATAGACCATTTCGGAATGGCATATACATCACACATCCTGGATCAAGTAAAGACTCTAGGGTTCCGTCAAGCTACTGCTACATCTATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAAGGATGGCTAGTCCAAGATGCTGAACAACAAAGTTCTATTTTGGAAAAACATAATCAGTATGGGAATGTACATGCGGTAGAAAAATTACGCCAATCCATTGAAATATGGTATGCTACAAGCGAATATTTGCGACAAGAAATGAATCCTAATTTTAGGATGACTGACCCCTTTAATCCAGTCCATATAATGTCTTTTTCAGGAGCTAGAGGAAATGCATCTCAAGTGCACCAATTAGTCGGAATGAGAGGATTAATGTCAGATCCACAAGGACAAATGATTGATTTACCCATTCAAAGCAATTTACGAGAAGGACTGTCTTTAACAGAATATATAATTTCTTGCTACGGAGCCCGTAAAGGGGTTGTGGATACTGCTGTACGAACATCAGATGCTGGATACCGGATTAATAGTATTCTGGGCCGGAGCAATGAACCTATTTGA |
| Protein sequence |
>MsG0380013962.01.T01 MEVLMAERANLVFHNNVIGGTAIKRLISRLIDHFGMAYTSHILDQVKTLGFRQATATSISLGIDDLLTIPSKGWLVQDAEQQSSILEKHNQYGNVHAVEKLRQSIEIWYATSEYLRQEMNPNFRMTDPFNPVHIMSFSGARGNASQVHQLVGMRGLMSDPQGQMIDLPIQSNLREGLSLTEYIISCYGARKGVVDTAVRTSDAGYRINSILGRSNEPI* |
