Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014032.01.T01 | KAG8363205.1 | 98.63 | 292 | 4 | 0 | 1 | 292 | 1 | 292 | 0 | 587 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014032.01.T01 | A0A649X4Q6 | 98.630 | 292 | 4 | 0 | 1 | 292 | 1 | 292 | 0.0 | 587 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047967.01 | MsG0380014032.01 | 0.920667 | 9.573590e-88 | 3.287232e-83 |
| MsG0080048036.01 | MsG0380014032.01 | -0.805104 | 1.567915e-49 | 8.224333e-47 |
| MsG0080048059.01 | MsG0380014032.01 | 0.881126 | 3.109075e-70 | 1.799374e-66 |
| MsG0080048112.01 | MsG0380014032.01 | -0.809621 | 1.728083e-50 | 1.019649e-47 |
| MsG0080048302.01 | MsG0380014032.01 | -0.849617 | 2.882404e-60 | 5.496276e-57 |
| MsG0080048313.01 | MsG0380014032.01 | 0.810215 | 1.287723e-50 | 7.716367e-48 |
| MsG0080048560.01 | MsG0380014032.01 | 0.848521 | 5.811956e-60 | 1.069549e-56 |
| MsG0080048690.01 | MsG0380014032.01 | 0.884781 | 1.429701e-71 | 9.560698e-68 |
| MsG0080048691.01 | MsG0380014032.01 | 0.867128 | 1.719863e-65 | 5.937939e-62 |
| MsG0080048692.01 | MsG0380014032.01 | 0.956228 | 4.838561e-114 | 1.546491e-108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014032.01.T01 | MTR_0002s0420 | 95.548 | 292 | 13 | 0 | 1 | 292 | 1 | 292 | 0.0 | 570 |
| MsG0380014032.01.T01 | MTR_4g073360 | 95.255 | 274 | 13 | 0 | 19 | 292 | 1 | 274 | 0.0 | 531 |
| MsG0380014032.01.T01 | MTR_5g094930 | 97.015 | 67 | 2 | 0 | 183 | 249 | 30 | 96 | 1.99e-41 | 140 |
| MsG0380014032.01.T01 | MTR_7g021750 | 30.769 | 286 | 187 | 6 | 10 | 287 | 8 | 290 | 3.90e-41 | 146 |
| MsG0380014032.01.T01 | MTR_4g051290 | 30.847 | 295 | 191 | 7 | 1 | 287 | 1 | 290 | 4.24e-41 | 146 |
| MsG0380014032.01.T01 | MTR_0002s0380 | 30.420 | 286 | 188 | 6 | 10 | 287 | 8 | 290 | 6.17e-40 | 143 |
| MsG0380014032.01.T01 | MTR_6g033745 | 46.528 | 144 | 35 | 4 | 1 | 110 | 1 | 136 | 5.08e-31 | 116 |
Find 70 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCAACCCCAAAGATTT+GGG | 0.380424 | 3:+48693851 | None:intergenic |
| AAATGATTTATTTGATATTA+TGG | 0.174845 | 3:-48694579 | MsG0380014032.01.T01:CDS |
| AACCAACCAGACTGACCTAG+TGG | 0.633960 | 3:+48694131 | None:intergenic |
| AAGTTTCTTCCGCTTGAGTT+GGG | 0.471818 | 3:+48693904 | None:intergenic |
| AATAGGTATTGGTATTTATC+CGG | 0.384488 | 3:-48693380 | MsG0380014032.01.T01:intron |
| ACACTCTTTGTTGTTACTAT+GGG | 0.376237 | 3:-48694354 | MsG0380014032.01.T01:CDS |
| ACCACCCAATTGACACCAAC+GGG | 0.447715 | 3:+48694307 | None:intergenic |
| ACCATGAATAGCGCATAGTA+GGG | 0.553237 | 3:+48693989 | None:intergenic |
| ACCCGTTGGTGTCAATTGGG+TGG | 0.554774 | 3:-48694308 | MsG0380014032.01.T01:CDS |
| ACGTTATTTGAAGATGGTGA+TGG | 0.581496 | 3:-48693951 | MsG0380014032.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAGAAACAAAAACAGCAAT+TGG | + | Chr3:48693774-48693793 | None:intergenic | 30.0% | |
| AGAATGCGATTGCATTATAA+GGG | + | Chr3:48693746-48693765 | None:intergenic | 30.0% | |
| AGTATACCATGAAGTTACAA+AGG | + | Chr3:48693487-48693506 | None:intergenic | 30.0% | |
| GAAATGTAACCAACGTTTAT+TGG | + | Chr3:48694114-48694133 | None:intergenic | 30.0% | |
| TTGTGAATCCATATTATGAG+CGG | - | Chr3:48694299-48694318 | MsG0380014032.01.T01:CDS | 30.0% | |
| AAAAAGCAACCCCAAAGATT+TGG | + | Chr3:48694091-48694110 | None:intergenic | 35.0% | |
| AAAAGCAACCCCAAAGATTT+GGG | + | Chr3:48694090-48694109 | None:intergenic | 35.0% | |
| AACAAAAACAGCAATTGGAC+CGG | + | Chr3:48693769-48693788 | None:intergenic | 35.0% | |
| AAGAGTAAATATTGCCTCGA+TGG | + | Chr3:48694271-48694290 | None:intergenic | 35.0% | |
| ACCAACCCACAAAAACAAAA+CGG | + | Chr3:48693404-48693423 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48693324 | 48694636 | 48693324 | ID=MsG0380014032.01;Name=MsG0380014032.01 |
| Chr3 | mRNA | 48693324 | 48694636 | 48693324 | ID=MsG0380014032.01.T01;Parent=MsG0380014032.01;Name=MsG0380014032.01.T01;_AED=0.50;_eAED=0.96;_QI=0|0|0|1|1|1|2|0|312 |
| Chr3 | exon | 48693772 | 48694636 | 48693772 | ID=MsG0380014032.01.T01:exon:56983;Parent=MsG0380014032.01.T01 |
| Chr3 | exon | 48693324 | 48693397 | 48693324 | ID=MsG0380014032.01.T01:exon:56982;Parent=MsG0380014032.01.T01 |
| Chr3 | CDS | 48693772 | 48694636 | 48693772 | ID=MsG0380014032.01.T01:cds;Parent=MsG0380014032.01.T01 |
| Chr3 | CDS | 48693324 | 48693397 | 48693324 | ID=MsG0380014032.01.T01:cds;Parent=MsG0380014032.01.T01 |
| Gene Sequence |
>MsG0380014032.01.T01 ATGACTATAGCTCTTGGTAAATTTACCAAAGATGAAAATGATTTATTTGATATTATGGATGACTGGTTACGGAGGGACCGTTTTGTTTTTGTGGGTTGGTCCGGTCTATTGCTCTTTCCTTGCGCCTATTTTGCCGTGGGGGGTTGGTTCACAGGTACCACCTTTGTAACTTCATGGTATACTCATGGATTGGCAAGTTCCTATTTGGAAGGTTGTAACTTCTTAACTGCGGCAGTCTCTACTCCTGCTAATAGTTTAGCACACTCTTTGTTGTTACTATGGGGTCCTGAAGCACAGGGAGATTTTACCCGTTGGTGTCAATTGGGTGGTCTGTGGACTTTTGTTGCTCTCCACGGTGCTTTCGGATTAATCGGTTTTATGTTACGTCAATTTGAACTTGCTCGATCTGTTCAATTGCGCCCTTATAATGCAATCGCATTCTCCGGTCCAATTGCTGTTTTTGTTTCTGTATTCCTGATTTATCCACTAGGTCAGTCTGGTTGGTTCTTTGCGCCTAGTTTTGGTGTAGCAGCTATATTTCGATTCATTCTATTTTTCCAAGGGTTTCATAATTGGACATTAAACCCATTTCATATGATGGGAGTTGCTGGTGTATTGGGCGCTGCCCTACTATGCGCTATTCATGGTGCTACCGTAGAAAATACGTTATTTGAAGATGGTGATGGCGCAAATACATTCCGTGCTTTTAACCCAACTCAAGCGGAAGAAACTTATTCAATGGTCACCGCTAACCACTTTTGGTCCCAAATCTTTGGGGTTGCTTTTTCCAATAAACGTTGGTTACATTTCTTTATGTTATTTGTACCAGTAACTGGTTTATGGATGAGTGCTCTTGGAGTAGTCGGTATTGGTATTTATCCGGATTTCATTTTTTCATTCTCGGTTGACAAGGTTGAAGCTATTCTAGCTAATTTTTGA |
| Protein sequence |
>MsG0380014032.01.T01 MTIALGKFTKDENDLFDIMDDWLRRDRFVFVGWSGLLLFPCAYFAVGGWFTGTTFVTSWYTHGLASSYLEGCNFLTAAVSTPANSLAHSLLLLWGPEAQGDFTRWCQLGGLWTFVALHGAFGLIGFMLRQFELARSVQLRPYNAIAFSGPIAVFVSVFLIYPLGQSGWFFAPSFGVAAIFRFILFFQGFHNWTLNPFHMMGVAGVLGAALLCAIHGATVENTLFEDGDGANTFRAFNPTQAEETYSMVTANHFWSQIFGVAFSNKRWLHFFMLFVPVTGLWMSALGVVGIGIYPDFIFSFSVDKVEAILANF* |
