AlfalfaGEDB | MsG0080048313.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048313.01

KEGG: -
GO: GO:0005215 GO:0006814 GO:0016020 GO:0055085
PFAM: PF00939

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048313.01.T01	XP_003593234.1	65.359	153	8	3	1	108	209	361	2.19E-44	163

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048313.01.T01	Q8L7Z9	53.595	153	26	2	1	108	206	358	2.48E-37	137

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048313.01.T01	G7IPL2	65.359	153	8	3	1	108	209	361	1.05e-44	163

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048059.01	MsG0080048313.01	0.835184	1.945450e-56	2.360881e-53
MsG0080048313.01	MsG0080048690.01	0.814040	1.886704e-51	1.252373e-48
MsG0080048313.01	MsG0080048691.01	0.810413	1.166928e-50	7.029249e-48
MsG0080048313.01	MsG0080048698.01	0.827787	1.290109e-54	1.258093e-51
MsG0080048313.01	MsG0080049018.01	0.801844	7.433452e-49	3.586689e-46
MsG0080048313.01	MsG0080049076.01	0.802050	6.742167e-49	3.270210e-46
MsG0080048313.01	MsG0080049133.01	0.829147	6.056297e-55	6.145543e-52
MsG0080048313.01	MsG0180001925.01	0.825405	4.771888e-54	4.345432e-51
MsG0080048313.01	MsG0180002099.01	0.827645	1.395679e-54	1.355375e-51
MsG0080048313.01	MsG0180002214.01	0.830044	3.665425e-55	3.817955e-52
MsG0080048313.01	MsG0180002426.01	0.826673	2.384979e-54	2.251702e-51
MsG0080048313.01	MsG0180002546.01	0.808758	2.646441e-50	1.525819e-47
MsG0080048313.01	MsG0180002809.01	0.808372	3.198543e-50	1.825756e-47
MsG0080048313.01	MsG0180003114.01	0.826798	2.225759e-54	2.108904e-51
MsG0080048313.01	MsG0180003350.01	0.802315	5.946006e-49	2.903750e-46
MsG0080048313.01	MsG0180003985.01	0.802896	4.512558e-49	2.236468e-46
MsG0080048313.01	MsG0180004177.01	0.800814	1.207742e-48	5.677302e-46
MsG0080048313.01	MsG0180004292.01	0.803915	2.775235e-49	1.411928e-46
MsG0080048313.01	MsG0180004301.01	0.858744	6.675037e-63	1.721239e-59
MsG0080048313.01	MsG0180004435.01	0.860486	1.997293e-63	5.468688e-60
MsG0080048313.01	MsG0180004553.01	0.810819	9.533249e-51	5.805449e-48
MsG0080048313.01	MsG0180004580.01	0.800274	1.556351e-48	7.216656e-46
MsG0080048313.01	MsG0180004581.01	0.802477	5.507624e-49	2.700536e-46
MsG0080048313.01	MsG0180004747.01	0.807450	5.023975e-50	2.799258e-47
MsG0080048313.01	MsG0180004761.01	0.832910	7.216766e-56	8.180526e-53
MsG0080048313.01	MsG0180004852.01	0.802069	6.682934e-49	3.242995e-46
MsG0080048313.01	MsG0180004957.01	0.837075	6.435961e-57	8.267267e-54
MsG0080048313.01	MsG0180005223.01	0.802738	4.866231e-49	2.402044e-46
MsG0080048313.01	MsG0180005242.01	0.821170	4.655909e-53	3.759703e-50
MsG0080048313.01	MsG0180005431.01	0.802331	5.902853e-49	2.883749e-46
MsG0080048313.01	MsG0180005516.01	0.812438	4.239740e-51	2.695895e-48
MsG0080048313.01	MsG0180005791.01	0.821068	4.915158e-53	3.958166e-50
MsG0080048313.01	MsG0180005817.01	0.806100	9.689941e-50	5.214298e-47
MsG0080048313.01	MsG0180006212.01	0.840603	7.871736e-58	1.126971e-54
MsG0080048313.01	MsG0180006227.01	0.821598	3.708329e-53	3.030299e-50
MsG0080048313.01	MsG0280006452.01	0.803269	3.778521e-49	1.890547e-46
MsG0080048313.01	MsG0280006475.01	0.892455	1.562695e-74	1.429790e-70
MsG0080048313.01	MsG0280007136.01	0.826146	3.183980e-54	2.961224e-51
MsG0080048313.01	MsG0280007247.01	0.804042	2.611323e-49	1.332812e-46
MsG0080048313.01	MsG0280007649.01	0.814923	1.203177e-51	8.178923e-49
MsG0080048313.01	MsG0280007650.01	0.818728	1.684994e-52	1.271374e-49
MsG0080048313.01	MsG0280008912.01	0.819414	1.176106e-52	9.044350e-50
MsG0080048313.01	MsG0280009541.01	0.818161	2.264817e-52	1.682355e-49
MsG0080048313.01	MsG0280009971.01	-0.813057	3.102990e-51	2.005455e-48
MsG0080048313.01	MsG0280010137.01	0.806305	8.775500e-50	4.746951e-47
MsG0080048313.01	MsG0280010145.01	0.822271	2.589595e-53	2.157242e-50
MsG0080048313.01	MsG0280010281.01	0.802690	4.978172e-49	2.454232e-46
MsG0080048313.01	MsG0380012281.01	0.800018	1.754302e-48	8.081847e-46
MsG0080048313.01	MsG0380013448.01	0.843243	1.579334e-58	2.455764e-55
MsG0080048313.01	MsG0380013473.01	0.809183	2.145603e-50	1.251245e-47
MsG0080048313.01	MsG0380013917.01	0.803709	3.062412e-49	1.549624e-46
MsG0080048313.01	MsG0380013929.01	0.801964	7.022567e-49	3.398541e-46
MsG0080048313.01	MsG0380013931.01	0.816177	6.324532e-52	4.449725e-49
MsG0080048313.01	MsG0380013935.01	0.801683	8.021437e-49	3.854376e-46
MsG0080048313.01	MsG0380013940.01	0.810223	1.282337e-50	7.685759e-48
MsG0080048313.01	MsG0380013942.01	0.815641	8.332251e-52	5.776982e-49
MsG0080048313.01	MsG0380013943.01	0.827184	1.799928e-54	1.724883e-51
MsG0080048313.01	MsG0380013945.01	0.807809	4.215267e-50	2.370629e-47
MsG0080048313.01	MsG0380013952.01	0.813356	2.668462e-51	1.738765e-48
MsG0080048313.01	MsG0380013953.01	0.800899	1.160762e-48	5.468625e-46
MsG0080048313.01	MsG0380013956.01	0.824558	7.564784e-54	6.724049e-51
MsG0080048313.01	MsG0380013957.01	0.815412	9.366105e-52	6.452829e-49
MsG0080048313.01	MsG0380013966.01	0.825810	3.827053e-54	3.525583e-51
MsG0080048313.01	MsG0380013967.01	0.816694	4.846919e-52	3.458410e-49
MsG0080048313.01	MsG0380013969.01	0.804412	2.186879e-49	1.126785e-46
MsG0080048313.01	MsG0380013971.01	0.828886	7.004420e-55	7.052749e-52
MsG0080048313.01	MsG0380013977.01	0.821530	3.844960e-53	3.136015e-50
MsG0080048313.01	MsG0380014005.01	0.821611	3.681817e-53	3.009850e-50
MsG0080048313.01	MsG0380014018.01	0.811242	7.720339e-51	4.754269e-48
MsG0080048313.01	MsG0380014020.01	0.803346	3.642542e-49	1.826080e-46
MsG0080048313.01	MsG0380014023.01	0.852743	3.777408e-61	7.971745e-58
MsG0080048313.01	MsG0380014032.01	0.810215	1.287723e-50	7.716367e-48
MsG0080048313.01	MsG0380014034.01	0.801116	1.047770e-48	4.963508e-46
MsG0080048313.01	MsG0380015200.01	0.838607	2.601575e-57	3.502694e-54
MsG0080048313.01	MsG0380015201.01	0.819523	1.110687e-52	8.567483e-50
MsG0080048313.01	MsG0380015348.01	0.843861	1.079645e-58	1.711917e-55
MsG0080048313.01	MsG0380015439.01	0.822512	2.276492e-53	1.909545e-50
MsG0080048313.01	MsG0380015697.01	0.800628	1.318278e-48	6.167945e-46
MsG0080048313.01	MsG0380015752.01	0.807938	3.958279e-50	2.233546e-47
MsG0080048313.01	MsG0380016056.01	0.814384	1.583759e-51	1.061178e-48
MsG0080048313.01	MsG0380016089.01	0.812757	3.611204e-51	2.315359e-48
MsG0080048313.01	MsG0380016229.01	0.811851	5.693093e-51	3.562661e-48
MsG0080048313.01	MsG0380016881.01	0.832628	8.480027e-56	9.532177e-53
MsG0080048313.01	MsG0380016889.01	0.830812	2.378298e-55	2.533481e-52
MsG0080048313.01	MsG0380017317.01	0.822914	1.835556e-53	1.557335e-50
MsG0080048313.01	MsG0380017405.01	0.823238	1.542656e-53	1.320755e-50
MsG0080048313.01	MsG0380017406.01	0.815802	7.670680e-52	5.341750e-49
MsG0080048313.01	MsG0380017647.01	0.818505	1.893082e-52	1.419644e-49
MsG0080048313.01	MsG0380017775.01	0.813328	2.705614e-51	1.761603e-48
MsG0080048313.01	MsG0380017928.01	0.821603	3.698401e-53	3.022673e-50
MsG0080048313.01	MsG0380017962.01	0.811604	6.442507e-51	4.005240e-48
MsG0080048313.01	MsG0380018047.01	0.819956	8.846579e-53	6.906922e-50
MsG0080048313.01	MsG0480018088.01	0.814623	1.402008e-51	9.454447e-49
MsG0080048313.01	MsG0480018628.01	0.801059	1.076611e-48	5.092820e-46
MsG0080048313.01	MsG0480018888.01	0.837363	5.434391e-57	7.042249e-54
MsG0080048313.01	MsG0480019861.01	0.823155	1.613142e-53	1.377905e-50
MsG0080048313.01	MsG0480019975.01	0.858955	5.774376e-63	1.499858e-59
MsG0080048313.01	MsG0480020076.01	0.800110	1.680408e-48	7.759525e-46
MsG0080048313.01	MsG0480021054.01	0.867236	1.588478e-65	5.506306e-62
MsG0080048313.01	MsG0480021327.01	0.834829	2.390766e-56	2.870576e-53
MsG0080048313.01	MsG0480021331.01	0.836927	7.023886e-57	8.982612e-54
MsG0080048313.01	MsG0480021474.01	0.840180	1.015558e-57	1.435152e-54
MsG0080048313.01	MsG0480021476.01	0.823318	1.477216e-53	1.267587e-50
MsG0080048313.01	MsG0480021912.01	0.815448	9.197332e-52	6.342922e-49
MsG0080048313.01	MsG0480022581.01	0.835924	1.263805e-56	1.568100e-53
MsG0080048313.01	MsG0480022591.01	0.815496	8.974531e-52	6.197630e-49
MsG0080048313.01	MsG0480022626.01	0.811344	7.336698e-51	4.529959e-48
MsG0080048313.01	MsG0480022665.01	0.808522	2.971479e-50	1.702800e-47
MsG0080048313.01	MsG0480023006.01	0.830050	3.653097e-55	3.805675e-52
MsG0080048313.01	MsG0480023217.01	0.817602	3.029038e-52	2.215772e-49
MsG0080048313.01	MsG0480023480.01	0.824653	7.182987e-54	6.402792e-51
MsG0080048313.01	MsG0480023481.01	0.830386	3.023254e-55	3.180492e-52
MsG0080048313.01	MsG0480023482.01	0.803899	2.797095e-49	1.422411e-46
MsG0080048313.01	MsG0480023818.01	0.802477	5.507624e-49	2.700536e-46
MsG0080048313.01	MsG0480023850.01	0.840898	6.589166e-58	9.520735e-55
MsG0080048313.01	MsG0580024278.01	0.827398	1.599626e-54	1.542665e-51
MsG0080048313.01	MsG0580024372.01	0.800666	1.295093e-48	6.065290e-46
MsG0080048313.01	MsG0580024570.01	0.803720	3.046842e-49	1.542203e-46
MsG0080048313.01	MsG0580027325.01	0.823176	1.594688e-53	1.362961e-50
MsG0080048313.01	MsG0580027677.01	0.806322	8.702406e-50	4.709455e-47
MsG0080048313.01	MsG0580030237.01	0.816072	6.675582e-52	4.683295e-49
MsG0080048313.01	MsG0680030439.01	0.822335	2.503234e-53	2.089163e-50
MsG0080048313.01	MsG0680030875.01	0.831836	1.331730e-55	1.462473e-52
MsG0080048313.01	MsG0680030879.01	0.820079	8.289562e-53	6.494814e-50
MsG0080048313.01	MsG0680032197.01	0.823571	1.289018e-53	1.114067e-50
MsG0080048313.01	MsG0780038047.01	0.823876	1.093599e-53	9.534135e-51
MsG0080048313.01	MsG0780038197.01	0.818044	2.406468e-52	1.781803e-49
MsG0080048313.01	MsG0780038347.01	0.829772	4.269912e-55	4.413528e-52
MsG0080048313.01	MsG0780039838.01	0.812870	3.410593e-51	2.193176e-48
MsG0080048313.01	MsG0780039839.01	0.806495	8.003133e-50	4.350122e-47
MsG0080048313.01	MsG0780039879.01	0.817022	4.089739e-52	2.944765e-49
MsG0080048313.01	MsG0780040121.01	0.814684	1.359454e-51	9.182391e-49
MsG0080048313.01	MsG0780040316.01	0.803625	3.187514e-49	1.609521e-46
MsG0080048313.01	MsG0780040321.01	0.804211	2.408497e-49	1.234568e-46
MsG0080048313.01	MsG0780040437.01	0.819616	1.057981e-52	8.181462e-50
MsG0080048313.01	MsG0780040622.01	0.820862	5.479792e-53	4.387455e-50
MsG0080048313.01	MsG0780040644.01	0.800707	1.270054e-48	5.953969e-46
MsG0080048313.01	MsG0780040838.01	0.827055	1.932249e-54	1.844583e-51
MsG0080048313.01	MsG0780041121.01	0.808858	2.518693e-50	1.456029e-47
MsG0080048313.01	MsG0780041173.01	0.827885	1.221724e-54	1.194900e-51
MsG0080048313.01	MsG0780041254.01	0.804678	1.924977e-49	9.986940e-47
MsG0080048313.01	MsG0780041620.01	0.811751	5.984183e-51	3.735002e-48
MsG0080048313.01	MsG0780041637.01	0.840514	8.307599e-58	1.186185e-54
MsG0080048313.01	MsG0780041783.01	0.812113	4.992752e-51	3.146630e-48
MsG0080048313.01	MsG0880042895.01	0.814210	1.730141e-51	1.153716e-48
MsG0080048313.01	MsG0880044339.01	0.803686	3.096763e-49	1.566115e-46
MsG0080048313.01	MsG0880045188.01	0.847466	1.136168e-59	2.021367e-56
MsG0080048313.01	MsG0880045374.01	0.863739	2.007135e-64	6.150576e-61
MsG0080048313.01	MsG0880045413.01	0.824954	6.099165e-54	5.483542e-51
MsG0080048313.01	MsG0880045414.01	0.832821	7.595592e-56	8.586876e-53
MsG0080048313.01	MsG0880045630.01	0.823380	1.429043e-53	1.228317e-50
MsG0080048313.01	MsG0880046099.01	0.810942	8.970064e-51	5.480671e-48
MsG0080048313.01	MsG0880046105.01	0.801212	1.001458e-48	4.755468e-46
MsG0080048313.01	MsG0880046219.01	0.808934	2.426336e-50	1.405432e-47
MsG0080048313.01	MsG0880046462.01	0.810104	1.360541e-50	8.129887e-48
MsG0080048313.01	MsG0880046469.01	0.826866	2.144031e-54	2.035456e-51
MsG0080048313.01	MsG0880046584.01	0.825731	3.993854e-54	3.670977e-51
MsG0080048313.01	MsG0880046639.01	0.833088	6.518964e-56	7.429774e-53
MsG0080048313.01	MsG0880047318.01	0.808526	2.965495e-50	1.699549e-47
MsG0080048313.01	MsG0880047661.01	0.804219	2.399788e-49	1.230338e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048313.01.T01	MTR_2g009220	65.359	153	8	3	1	108	209	361	2.65e-48	163
MsG0080048313.01.T01	MTR_4g065007	40.000	150	46	3	2	107	201	350	2.54e-24	97.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048313.01.T01	AT5G64290	53.595	153	26	3	1	108	205	357	1.53e-36	132
MsG0080048313.01.T01	AT5G64280	48.366	153	34	3	1	108	191	343	1.51e-28	109
MsG0080048313.01.T01	AT5G12860	37.748	151	50	3	2	108	201	351	5.93e-23	94.0
MsG0080048313.01.T01	AT5G12860	37.748	151	50	3	2	108	201	351	6.28e-23	94.0

Find 59 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTAGGTATTAATTATTA+TGG	0.191954	contig271end:-158185	None:intergenic
TGCCCTGGCTGCAAAGAAAT+TGG	0.239587	contig271end:+155755	MsG0080048313.01.T01:CDS
GAGAAATGCTATTTACTTAC+AGG	0.241161	contig271end:-157967	None:intergenic
ATGGGGTGGTGTGGCTCATC+TGG	0.266876	contig271end:+157908	MsG0080048313.01.T01:CDS
TAAAACCCGTTTGAATATAT+CGG	0.280522	contig271end:-157854	None:intergenic
GCAGCCAGGGCAGGTGCCTC+TGG	0.321173	contig271end:-155744	None:intergenic
TCTCTATGCTTCTTTATTCC+CGG	0.323599	contig271end:+157986	MsG0080048313.01.T01:three_prime_UTR
AAATGAAGGACCTTATCTTT+TGG	0.332746	contig271end:-158009	None:intergenic
AGTGAAAGTGACTTAATAAT+AGG	0.342395	contig271end:-154335	None:intergenic
TCTGGCGTGTCTTTAATTTC+AGG	0.344554	contig271end:-155726	None:intergenic
AGTACTTTAGGACTTTCTTA+TGG	0.358141	contig271end:+154239	MsG0080048313.01.T01:CDS
CATTATTAAACTAACAAATA+AGG	0.371345	contig271end:-158083	None:intergenic
TTTAAATTAGCACAGAGCTT+GGG	0.377989	contig271end:+155603	MsG0080048313.01.T01:intron
GGGGTGTAATTTCAAACCCT+TGG	0.383832	contig271end:+155623	MsG0080048313.01.T01:CDS
CAGTGAAGGTTTATTAAGTA+AGG	0.394095	contig271end:-158119	None:intergenic
TGGATGGGGAAGAGTACTTT+AGG	0.402380	contig271end:+154227	None:intergenic
TAATGCTGTCATCTGGGGAT+GGG	0.402607	contig271end:+157890	MsG0080048313.01.T01:CDS
AATGCTGTCATCTGGGGATG+GGG	0.416058	contig271end:+157891	MsG0080048313.01.T01:CDS
GGGTGTAATTTCAAACCCTT+GGG	0.431963	contig271end:+155624	MsG0080048313.01.T01:CDS
CACGCCAGAGGCACCTGCCC+TGG	0.434388	contig271end:+155740	MsG0080048313.01.T01:CDS
TTAATGCTGTCATCTGGGGA+TGG	0.446188	contig271end:+157889	MsG0080048313.01.T01:CDS
GGGTGGTGTGGCTCATCTGG+TGG	0.446367	contig271end:+157911	MsG0080048313.01.T01:CDS
AATTTCTTTGCAGCCAGGGC+AGG	0.450007	contig271end:-155753	None:intergenic
TGCAAAGAAATTGGAAACAA+TGG	0.450500	contig271end:+155764	MsG0080048313.01.T01:CDS
AACCCACTCATTTGAGTGAC+AGG	0.460306	contig271end:-155789	None:intergenic
CCTTCCCGATATATTCAAAC+GGG	0.461484	contig271end:+157849	MsG0080048313.01.T01:CDS
CCCAGCACATGGTGAAAACT+AGG	0.473131	contig271end:-158200	None:intergenic
CCAGCTCTAGCAGTAGTACT+AGG	0.475907	contig271end:-154299	None:intergenic
TTATTCCCGGCCAAAAGATA+AGG	0.478600	contig271end:+157999	MsG0080048313.01.T01:three_prime_UTR
GCAAAGAAATTGGAAACAAT+GGG	0.479542	contig271end:+155765	MsG0080048313.01.T01:CDS
TATTAATTATTATGGAATTG+TGG	0.481152	contig271end:-158177	None:intergenic
AAAACCCGTTTGAATATATC+GGG	0.481290	contig271end:-157853	None:intergenic
GCAGTAGTACTAGGCATAGC+AGG	0.484964	contig271end:-154290	None:intergenic
TTTCCAATTTCTTTGCAGCC+AGG	0.488995	contig271end:-155758	None:intergenic
ACCTTCCCGATATATTCAAA+CGG	0.489269	contig271end:+157848	MsG0080048313.01.T01:CDS
TACTACAATGATTTGTATGC+GGG	0.489373	contig271end:+158145	MsG0080048313.01.T01:three_prime_UTR
CATCTGGGGATGGGGTGGTG+TGG	0.498772	contig271end:+157899	MsG0080048313.01.T01:CDS
GCTGTCATCTGGGGATGGGG+TGG	0.501738	contig271end:+157894	MsG0080048313.01.T01:CDS
GTTTAAATTAGCACAGAGCT+TGG	0.502425	contig271end:+155602	MsG0080048313.01.T01:intron
AAGTCACTTTCACTCTCTGC+TGG	0.503849	contig271end:+154344	MsG0080048313.01.T01:CDS
TGCTTTAACCACGTAACCCA+AGG	0.505723	contig271end:-155640	None:intergenic
AAGATAAGGTCCTTCATTTG+AGG	0.524613	contig271end:+158013	MsG0080048313.01.T01:three_prime_UTR
TTCCAATTTCTTTGCAGCCA+GGG	0.535962	contig271end:-155757	None:intergenic
ACTCAACTGTCCTCAAATGA+AGG	0.550191	contig271end:-158023	None:intergenic
CCTAGTACTACTGCTAGAGC+TGG	0.554496	contig271end:+154299	MsG0080048313.01.T01:CDS
GCAAGAAGACAAACAAATGC+AGG	0.555336	contig271end:-155672	None:intergenic
TTAAATTAGCACAGAGCTTG+GGG	0.555815	contig271end:+155604	MsG0080048313.01.T01:intron
GGCGTGTCTTTAATTTCAGG+AGG	0.566930	contig271end:-155723	None:intergenic
GGCCCTGTCACTCAAATGAG+TGG	0.591478	contig271end:+155786	MsG0080048313.01.T01:CDS
TTTCAAACCCTTGGGTTACG+TGG	0.595590	contig271end:+155632	MsG0080048313.01.T01:CDS
CCCGTTTGAATATATCGGGA+AGG	0.603002	contig271end:-157849	None:intergenic
TCATTGTAGTATATCAGTGA+AGG	0.604278	contig271end:-158133	None:intergenic
GCTTTAACCACGTAACCCAA+GGG	0.624593	contig271end:-155639	None:intergenic
GCCCTGTCACTCAAATGAGT+GGG	0.629483	contig271end:+155787	MsG0080048313.01.T01:CDS
AGTACTACTGCTAGAGCTGG+TGG	0.631739	contig271end:+154302	MsG0080048313.01.T01:CDS
ATACTACAATGATTTGTATG+CGG	0.642613	contig271end:+158144	MsG0080048313.01.T01:three_prime_UTR
TAGATCGTGTATAAAATCAA+CGG	0.643604	contig271end:-155699	None:intergenic
ACCCACTCATTTGAGTGACA+GGG	0.661550	contig271end:-155788	None:intergenic
TGAAATTAAAGACACGCCAG+AGG	0.684057	contig271end:+155728	MsG0080048313.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTTTATTTTTATTTTTTA+TGG	+	contig271end:155048-155067	MsG0080048313.01.T01:intron	0.0%
!!	AACAATTCAATTATAAAATT+TGG	+	contig271end:154954-154973	MsG0080048313.01.T01:intron	10.0%
!!	ATATATTAGTAGTATTATAA+AGG	-	contig271end:156416-156435	None:intergenic	10.0%
!!	TATATTAGTAGTATTATAAA+GGG	-	contig271end:156415-156434	None:intergenic	10.0%
!!	TGAATTCTAATAAAATATTA+TGG	+	contig271end:156033-156052	MsG0080048313.01.T01:intron	10.0%
!!!	AAATGTTTTTATTCTTATAA+AGG	+	contig271end:157674-157693	MsG0080048313.01.T01:intron	10.0%
!!!	TAAGAATAAAAACATTTTTT+TGG	-	contig271end:157671-157690	None:intergenic	10.0%
!!	AAACTAGGTATTAATTATTA+TGG	-	contig271end:158188-158207	None:intergenic	15.0%
!!	ATAATACTACTAATATATCT+TGG	+	contig271end:156418-156437	MsG0080048313.01.T01:intron	15.0%
!!	ATATTAGTAGTATTATAAAG+GGG	-	contig271end:156414-156433	None:intergenic	15.0%
!!	ATTTCATCTAATAAATATAG+AGG	-	contig271end:154712-154731	None:intergenic	15.0%
!!	CATTATTAAACTAACAAATA+AGG	-	contig271end:158086-158105	None:intergenic	15.0%
!!	TATTAATTATTATGGAATTG+TGG	-	contig271end:158180-158199	None:intergenic	15.0%
!!!	AATAAAAACATTTTTTTGGA+AGG	-	contig271end:157667-157686	None:intergenic	15.0%
!!	ATATAAATAGAGGTAACTTA+AGG	+	contig271end:154988-155007	MsG0080048313.01.T01:intron	20.0%
!!	ATTGTTCATGAAAAAGAAAA+AGG	-	contig271end:154940-154959	None:intergenic	20.0%
!!	ATTTAACCAAATAATCACTT+AGG	+	contig271end:155147-155166	MsG0080048313.01.T01:intron	20.0%
!!	TACTAATATATCTTGGAATA+CGG	+	contig271end:156425-156444	MsG0080048313.01.T01:intron	20.0%
!!	TGTAAAATGTATTTCATCAT+TGG	+	contig271end:155258-155277	MsG0080048313.01.T01:intron	20.0%
!!	TTAGGCATATTAATATACTA+AGG	+	contig271end:156451-156470	MsG0080048313.01.T01:intron	20.0%
!!!	ATGATGAAATACATTTTACA+AGG	-	contig271end:155258-155277	None:intergenic	20.0%
!!!	TGTGTCTTTTATTCAAAATA+CGG	-	contig271end:154781-154800	None:intergenic	20.0%
!!!	TGTTAGTTTAATGTTATACA+CGG	+	contig271end:154491-154510	MsG0080048313.01.T01:intron	20.0%
!	AAAGAAAAACAGAAATCATG+TGG	-	contig271end:157357-157376	None:intergenic	25.0%
!	AATACATGTAACGTAAATGT+AGG	-	contig271end:157431-157450	None:intergenic	25.0%
!	AATTATGTATTCTCACTTCT+CGG	+	contig271end:155402-155421	MsG0080048313.01.T01:intron	25.0%
!	AGCAAATGACATATAAATAG+AGG	+	contig271end:154978-154997	MsG0080048313.01.T01:intron	25.0%
!	AGCTTATGAAAACAACTTAT+TGG	+	contig271end:156221-156240	MsG0080048313.01.T01:intron	25.0%
!	AGGTTATAATACCAATCTTT+CGG	+	contig271end:157258-157277	MsG0080048313.01.T01:intron	25.0%
!	AGTGAAAGTGACTTAATAAT+AGG	-	contig271end:154338-154357	None:intergenic	25.0%
!	ATACATGTAACGTAAATGTA+GGG	-	contig271end:157430-157449	None:intergenic	25.0%
!	ATCTAACATATATGTTAGCA+AGG	-	contig271end:154748-154767	None:intergenic	25.0%
!	ATCTATCCTAAGTGATTATT+TGG	-	contig271end:155156-155175	None:intergenic	25.0%
!	CCTTTAACTCACAAAAATTA+GGG	-	contig271end:154871-154890	None:intergenic	25.0%
!	GAAGTAGTTATGATTGAAAT+CGG	+	contig271end:154549-154568	MsG0080048313.01.T01:intron	25.0%
!	TAAAACCCGTTTGAATATAT+CGG	-	contig271end:157857-157876	None:intergenic	25.0%
!	TAGATCGTGTATAAAATCAA+CGG	-	contig271end:155702-155721	None:intergenic	25.0%
!	TCCTTTAACTCACAAAAATT+AGG	-	contig271end:154872-154891	None:intergenic	25.0%
!	TTCACGTATATTAAGAAAAG+TGG	-	contig271end:156525-156544	None:intergenic	25.0%
!	TTTAGAGACAAATTCTTCAA+AGG	-	contig271end:156345-156364	None:intergenic	25.0%
!!	ATACTACAATGATTTGTATG+CGG	+	contig271end:158144-158163	MsG0080048313.01.T01:three_prime_UTR	25.0%
!!!	ATTCAAACGGGTTTTATATA+TGG	+	contig271end:157861-157880	MsG0080048313.01.T01:CDS	25.0%
!!!	TATTGACTTTAGAACTAGTT+AGG	+	contig271end:154655-154674	MsG0080048313.01.T01:intron	25.0%
!!!	TCTGTTTTTAATGGTATAAC+TGG	+	contig271end:157475-157494	MsG0080048313.01.T01:intron	25.0%
!!!	TTTGATTTTATGAGACATGA+TGG	+	contig271end:155359-155378	MsG0080048313.01.T01:intron	25.0%
	AAAAACAGAAATGAAGCGTA+GGG	-	contig271end:157464-157483	None:intergenic	30.0%
	AAAACCCGTTTGAATATATC+GGG	-	contig271end:157856-157875	None:intergenic	30.0%
	AAACTCTAACACATAAAAGG+AGG	-	contig271end:156775-156794	None:intergenic	30.0%
	AACCGATTGTAATTTCTTGA+CGG	-	contig271end:154631-154650	None:intergenic	30.0%
	AATAAGCTGTTTATCCAAGT+GGG	+	contig271end:156615-156634	MsG0080048313.01.T01:intron	30.0%
	ATACGTGAAAAGAAAGTAAG+AGG	+	contig271end:156536-156555	MsG0080048313.01.T01:intron	30.0%
	ATGAGAAAGGAGCTTAAATA+TGG	-	contig271end:155986-156005	None:intergenic	30.0%
	ATTTAAGCTCCTTTCTCATA+TGG	+	contig271end:155987-156006	MsG0080048313.01.T01:intron	30.0%
	CAGTGAAGGTTTATTAAGTA+AGG	-	contig271end:158122-158141	None:intergenic	30.0%
	CTGACAATATTGAACTTTGA+AGG	+	contig271end:155923-155942	MsG0080048313.01.T01:intron	30.0%
	GCAAAACTCTAACACATAAA+AGG	-	contig271end:156778-156797	None:intergenic	30.0%
	GCAAAGAAATTGGAAACAAT+GGG	+	contig271end:155765-155784	MsG0080048313.01.T01:CDS	30.0%
	GCAATTAATCCATATGAGAA+AGG	-	contig271end:155999-156018	None:intergenic	30.0%
	TAAAAACAGAAATGAAGCGT+AGG	-	contig271end:157465-157484	None:intergenic	30.0%
	TAATAAGCTGTTTATCCAAG+TGG	+	contig271end:156614-156633	MsG0080048313.01.T01:intron	30.0%
	TCATTGTAGTATATCAGTGA+AGG	-	contig271end:158136-158155	None:intergenic	30.0%
	TCTTATTCAGAACCAATTTC+AGG	+	contig271end:154409-154428	MsG0080048313.01.T01:intron	30.0%
	TGATTGAGTAAATGATCTCA+TGG	+	contig271end:155895-155914	MsG0080048313.01.T01:intron	30.0%
	TGCAAAGAAATTGGAAACAA+TGG	+	contig271end:155764-155783	MsG0080048313.01.T01:CDS	30.0%
	TTCAAAGGAGCTTATAGTTA+GGG	-	contig271end:156330-156349	None:intergenic	30.0%
	TTTAAATTAGCACAGAGCTT+GGG	+	contig271end:155603-155622	MsG0080048313.01.T01:intron	30.0%
	TTTCCATCATGTTTAATGAG+AGG	-	contig271end:154809-154828	None:intergenic	30.0%
!	AAATGAAGGACCTTATCTTT+TGG	-	contig271end:158012-158031	None:intergenic	30.0%
!	ATTTTTAATGCTGTCATCTG+GGG	+	contig271end:157885-157904	MsG0080048313.01.T01:CDS	30.0%
!	CATTTTTAATGCTGTCATCT+GGG	+	contig271end:157884-157903	MsG0080048313.01.T01:CDS	30.0%
!	CCCTAATTTTTGTGAGTTAA+AGG	+	contig271end:154868-154887	MsG0080048313.01.T01:intron	30.0%
!	GAGAAATGCTATTTACTTAC+AGG	-	contig271end:157970-157989	None:intergenic	30.0%
!	GTTTTTAATGGTATAACTGG+TGG	+	contig271end:157478-157497	MsG0080048313.01.T01:intron	30.0%
!	TACTACAATGATTTGTATGC+GGG	+	contig271end:158145-158164	MsG0080048313.01.T01:three_prime_UTR	30.0%
!!	AGTACTTTAGGACTTTCTTA+TGG	+	contig271end:154239-154258	MsG0080048313.01.T01:CDS	30.0%
!!!	AATTCTTGTTTTTGAACAGC+TGG	+	contig271end:157817-157836	MsG0080048313.01.T01:intron	30.0%
!!!	CGCTTCATTTCTGTTTTTAA+TGG	+	contig271end:157466-157485	MsG0080048313.01.T01:intron	30.0%
!!!	CTCACTTTTTACTCTACTTA+AGG	+	contig271end:154445-154464	MsG0080048313.01.T01:intron	30.0%
!!!	TCGGATGTTTTTTAATGATC+TGG	+	contig271end:154568-154587	MsG0080048313.01.T01:intron	30.0%
	AAAAACAGAAATCATGTGGC+GGG	-	contig271end:157353-157372	None:intergenic	35.0%
	AAGATAAGGTCCTTCATTTG+AGG	+	contig271end:158013-158032	MsG0080048313.01.T01:three_prime_UTR	35.0%
	AATAAGCTGAAAACTCCCTA+TGG	-	contig271end:156179-156198	None:intergenic	35.0%
	ACATCTTATGACATGTCCAT+AGG	+	contig271end:156160-156179	MsG0080048313.01.T01:intron	35.0%
	ACCTTCCCGATATATTCAAA+CGG	+	contig271end:157848-157867	MsG0080048313.01.T01:CDS	35.0%
	AGAAAGGAGCTTAAATATGG+AGG	-	contig271end:155983-156002	None:intergenic	35.0%
	ATCATGTTTAATGAGAGGCA+AGG	-	contig271end:154804-154823	None:intergenic	35.0%
	ATGCACCAAGTAAATTGCAT+AGG	-	contig271end:156376-156395	None:intergenic	35.0%
	ATTTGTGCTTAAACCCAGTT+GGG	+	contig271end:157511-157530	MsG0080048313.01.T01:intron	35.0%
	CACCGTCAAGAAATTACAAT+CGG	+	contig271end:154626-154645	MsG0080048313.01.T01:intron	35.0%
	CATCTTATGACATGTCCATA+GGG	+	contig271end:156161-156180	MsG0080048313.01.T01:intron	35.0%
	CTTCAAAGGAGCTTATAGTT+AGG	-	contig271end:156331-156350	None:intergenic	35.0%
	GAAAAACAGAAATCATGTGG+CGG	-	contig271end:157354-157373	None:intergenic	35.0%
	GAGTAAATCCAACCTGAAAT+TGG	-	contig271end:154424-154443	None:intergenic	35.0%
	GCTTGAATGAAAATCAGCAT+GGG	+	contig271end:156967-156986	MsG0080048313.01.T01:intron	35.0%
	GTTTAAATTAGCACAGAGCT+TGG	+	contig271end:155602-155621	MsG0080048313.01.T01:intron	35.0%
	TATCTTGGAATACGGAAGTT+AGG	+	contig271end:156433-156452	MsG0080048313.01.T01:intron	35.0%
	TATTTGTGCTTAAACCCAGT+TGG	+	contig271end:157510-157529	MsG0080048313.01.T01:intron	35.0%
	TCTCTATGCTTCTTTATTCC+CGG	+	contig271end:157986-158005	MsG0080048313.01.T01:three_prime_UTR	35.0%
	TGAGCTAATTAGTTATTCGC+AGG	-	contig271end:157380-157399	None:intergenic	35.0%
	TGCACCAAGTAAATTGCATA+GGG	-	contig271end:156375-156394	None:intergenic	35.0%
	TGCTTGAATGAAAATCAGCA+TGG	+	contig271end:156966-156985	MsG0080048313.01.T01:intron	35.0%
	TGCTTGTAAAAAGAGATGGA+GGG	+	contig271end:156559-156578	MsG0080048313.01.T01:intron	35.0%
	TGTGAGTTAAAGGAATGACT+TGG	+	contig271end:154878-154897	MsG0080048313.01.T01:intron	35.0%
	TTAAATTAGCACAGAGCTTG+GGG	+	contig271end:155604-155623	MsG0080048313.01.T01:intron	35.0%
	TTGCCTCTCATTAAACATGA+TGG	+	contig271end:154803-154822	MsG0080048313.01.T01:intron	35.0%
	TTTACTGACATAAGCACTTG+TGG	+	contig271end:156119-156138	MsG0080048313.01.T01:intron	35.0%
!	ACCAAAGGCTATATTTGCTA+AGG	+	contig271end:157719-157738	MsG0080048313.01.T01:intron	35.0%
!	ATTCAGAACCAATTTCAGGT+TGG	+	contig271end:154413-154432	MsG0080048313.01.T01:intron	35.0%
!	GCATTTTTAATGCTGTCATC+TGG	+	contig271end:157883-157902	MsG0080048313.01.T01:CDS	35.0%
!!	CATGAAGCTTTTTCTGCTTT+TGG	+	contig271end:158058-158077	MsG0080048313.01.T01:three_prime_UTR	35.0%
!!	GTAGTGGATGAAGAAATAAG+CGG	-	contig271end:157137-157156	None:intergenic	35.0%
	AAAAGCTCCCAATCTCTTAG+AGG	-	contig271end:154390-154409	None:intergenic	40.0%
	AAAATCAGCATGGGATACCT+TGG	+	contig271end:156976-156995	MsG0080048313.01.T01:intron	40.0%
	AAGGAGCTTATAGTTAGGGA+CGG	-	contig271end:156326-156345	None:intergenic	40.0%
	ACAACTCAGATGATATTGCC+AGG	+	contig271end:155216-155235	MsG0080048313.01.T01:intron	40.0%
	ACTCAACTGTCCTCAAATGA+AGG	-	contig271end:158026-158045	None:intergenic	40.0%
	CAGTCTCTTTGTGGATCTTT+GGG	+	contig271end:155833-155852	MsG0080048313.01.T01:intron	40.0%
	CCTTCCCGATATATTCAAAC+GGG	+	contig271end:157849-157868	MsG0080048313.01.T01:CDS	40.0%
	CTGTTTATCCAAGTGGGAAA+AGG	+	contig271end:156621-156640	MsG0080048313.01.T01:intron	40.0%
	CTTATCCCAATAAGTGGAGT+GGG	-	contig271end:155304-155323	None:intergenic	40.0%
	CTTATTGGGATAAGGCTTAG+TGG	+	contig271end:155310-155329	MsG0080048313.01.T01:intron	40.0%
	GACTTGCACAGGTATAGTTT+GGG	-	contig271end:156746-156765	None:intergenic	40.0%
	GCAAGAAGACAAACAAATGC+AGG	-	contig271end:155675-155694	None:intergenic	40.0%
	GCAATTACACACTATAGCAG+TGG	+	contig271end:157282-157301	MsG0080048313.01.T01:intron	40.0%
	GCCTTAGCAAATATAGCCTT+TGG	-	contig271end:157723-157742	None:intergenic	40.0%
	GGGTGTAATTTCAAACCCTT+GGG	+	contig271end:155624-155643	MsG0080048313.01.T01:CDS	40.0%
	GTGCTTGTAAAAAGAGATGG+AGG	+	contig271end:156558-156577	MsG0080048313.01.T01:intron	40.0%
	GTGTAATTGCACCGAAAGAT+TGG	-	contig271end:157272-157291	None:intergenic	40.0%
	TAGCTGCAATTAGTGACCAA+AGG	+	contig271end:157704-157723	MsG0080048313.01.T01:intron	40.0%
	TCAGATAAGTCCATCCAAAC+AGG	-	contig271end:156094-156113	None:intergenic	40.0%
	TGAAATTAAAGACACGCCAG+AGG	+	contig271end:155728-155747	MsG0080048313.01.T01:CDS	40.0%
	TGACATAAGCACTTGTGGTT+GGG	+	contig271end:156124-156143	MsG0080048313.01.T01:intron	40.0%
	TTATTCCCGGCCAAAAGATA+AGG	+	contig271end:157999-158018	MsG0080048313.01.T01:three_prime_UTR	40.0%
	TTCCAATTTCTTTGCAGCCA+GGG	-	contig271end:155760-155779	None:intergenic	40.0%
	TTTCCAATTTCTTTGCAGCC+AGG	-	contig271end:155761-155780	None:intergenic	40.0%
!	ATTCTCTCCGGTGTCAATTT+GGG	+	contig271end:157594-157613	MsG0080048313.01.T01:intron	40.0%
!	ATTTCCATAAGCTGACTCAC+TGG	+	contig271end:156196-156215	MsG0080048313.01.T01:intron	40.0%
!	CTAAGCCTTATCCCAATAAG+TGG	-	contig271end:155310-155329	None:intergenic	40.0%
!	GAGGTGCTTGTAAAAAGAGA+TGG	+	contig271end:156555-156574	MsG0080048313.01.T01:intron	40.0%
!	GCTCATCTGGTGGAAATTTT+TGG	+	contig271end:157921-157940	MsG0080048313.01.T01:CDS	40.0%
!	TCTGGCGTGTCTTTAATTTC+AGG	-	contig271end:155729-155748	None:intergenic	40.0%
!	TGACTTGCACAGGTATAGTT+TGG	-	contig271end:156747-156766	None:intergenic	40.0%
!!	TCACTCAAATGAGTGGGTTA+TGG	+	contig271end:155793-155812	MsG0080048313.01.T01:intron	40.0%
	AACCCACTCATTTGAGTGAC+AGG	-	contig271end:155792-155811	None:intergenic	45.0%
	AATAAGTGGAGTGGGATGCA+TGG	-	contig271end:155296-155315	None:intergenic	45.0%
	ACAGAAATGAAGCGTAGGGA+AGG	-	contig271end:157460-157479	None:intergenic	45.0%
	ACCCACTCATTTGAGTGACA+GGG	-	contig271end:155791-155810	None:intergenic	45.0%
	AGCGCCCTATGCAATTTACT+TGG	+	contig271end:156368-156387	MsG0080048313.01.T01:intron	45.0%
	AGCTCAAGGAGAATGATCGA+AGG	-	contig271end:157086-157105	None:intergenic	45.0%
	AGCTTATAGTTAGGGACGGA+GGG	-	contig271end:156322-156341	None:intergenic	45.0%
	ATGCATCCCACTCCACTTAT+TGG	+	contig271end:155295-155314	MsG0080048313.01.T01:intron	45.0%
	ATGCTCTTGCAGTCTCTTTG+TGG	+	contig271end:155824-155843	MsG0080048313.01.T01:intron	45.0%
	ATTGGGATAAGGCTTAGTGG+TGG	+	contig271end:155313-155332	MsG0080048313.01.T01:intron	45.0%
	CAAGAAACACAGCAAGTGGA+AGG	-	contig271end:155462-155481	None:intergenic	45.0%
	CAAGCAAGAAACACAGCAAG+TGG	-	contig271end:155466-155485	None:intergenic	45.0%
	CAAGCTGCTGTATACTATGG+AGG	+	contig271end:157306-157325	MsG0080048313.01.T01:intron	45.0%
	CAAGTCAGTTGACAAACCTC+GGG	+	contig271end:157025-157044	MsG0080048313.01.T01:intron	45.0%
	CAGACTGTTACATGTCACAG+AGG	-	contig271end:155529-155548	None:intergenic	45.0%
	CCACTCCACTTATTGGGATA+AGG	+	contig271end:155302-155321	MsG0080048313.01.T01:intron	45.0%
	CCCGTTTGAATATATCGGGA+AGG	-	contig271end:157852-157871	None:intergenic	45.0%
	CCTTATCCCAATAAGTGGAG+TGG	-	contig271end:155305-155324	None:intergenic	45.0%
	CCTTGTCTTAGAGTCCTTGA+CGG	+	contig271end:156937-156956	MsG0080048313.01.T01:intron	45.0%
	CTATCCAGTGAGTCAGCTTA+TGG	-	contig271end:156203-156222	None:intergenic	45.0%
	CTCAGATGATATTGCCAGGT+TGG	+	contig271end:155220-155239	MsG0080048313.01.T01:intron	45.0%
	CTCTTAGAGGAGGGACTATT+AGG	-	contig271end:154377-154396	None:intergenic	45.0%
	CTGACATAAGCACTTGTGGT+TGG	+	contig271end:156123-156142	MsG0080048313.01.T01:intron	45.0%
	CTTGTCTTAGAGTCCTTGAC+GGG	+	contig271end:156938-156957	MsG0080048313.01.T01:intron	45.0%
	GAAGAAACAGCCAATGAAGC+CGG	-	contig271end:157111-157130	None:intergenic	45.0%
	GACTTGCATACCCACTAGAA+TGG	-	contig271end:157011-157030	None:intergenic	45.0%
	GCAAGTCAGTTGACAAACCT+CGG	+	contig271end:157024-157043	MsG0080048313.01.T01:intron	45.0%
	GCAGTCTCTTTGTGGATCTT+TGG	+	contig271end:155832-155851	MsG0080048313.01.T01:intron	45.0%
	GCTTTAACCACGTAACCCAA+GGG	-	contig271end:155642-155661	None:intergenic	45.0%
	GGGGTGTAATTTCAAACCCT+TGG	+	contig271end:155623-155642	MsG0080048313.01.T01:CDS	45.0%
	GGTCAAGCTGCTGTATACTA+TGG	+	contig271end:157303-157322	MsG0080048313.01.T01:intron	45.0%
	TAATGCTGTCATCTGGGGAT+GGG	+	contig271end:157890-157909	MsG0080048313.01.T01:CDS	45.0%
	TAGAATGGCAAACCAAGCCA+AGG	-	contig271end:156996-157015	None:intergenic	45.0%
	TGCATCCCACTCCACTTATT+GGG	+	contig271end:155296-155315	MsG0080048313.01.T01:intron	45.0%
	TGCGTTTCTTGCCATGCATA+GGG	+	contig271end:157198-157217	MsG0080048313.01.T01:intron	45.0%
	TGCTTTAACCACGTAACCCA+AGG	-	contig271end:155643-155662	None:intergenic	45.0%
	TGTGACATGTAACAGTCTGC+CGG	+	contig271end:155530-155549	MsG0080048313.01.T01:intron	45.0%
	TTAATGCTGTCATCTGGGGA+TGG	+	contig271end:157889-157908	MsG0080048313.01.T01:CDS	45.0%
	TTCGATCATTCTCCTTGAGC+TGG	+	contig271end:157085-157104	MsG0080048313.01.T01:intron	45.0%
	TTTCAAACCCTTGGGTTACG+TGG	+	contig271end:155632-155651	MsG0080048313.01.T01:CDS	45.0%
!	AAGGACCTTATCTTTTGGCC+GGG	-	contig271end:158007-158026	None:intergenic	45.0%
!	AAGTCACTTTCACTCTCTGC+TGG	+	contig271end:154344-154363	MsG0080048313.01.T01:CDS	45.0%
!	ACCTTTTTGCAAGCCAGACT+GGG	+	contig271end:157155-157174	MsG0080048313.01.T01:intron	45.0%
!	AGTCCCTCCTCTAAGAGATT+GGG	+	contig271end:154380-154399	MsG0080048313.01.T01:intron	45.0%
!	GAAGGACCTTATCTTTTGGC+CGG	-	contig271end:158008-158027	None:intergenic	45.0%
!	GATTCTCTCCGGTGTCAATT+TGG	+	contig271end:157593-157612	MsG0080048313.01.T01:intron	45.0%
!	GATTTTGAGCAGCAGCAGTT+AGG	-	contig271end:155574-155593	None:intergenic	45.0%
!	GGAGGTATAATGACTTGCAC+AGG	-	contig271end:156757-156776	None:intergenic	45.0%
!	GGCGTGTCTTTAATTTCAGG+AGG	-	contig271end:155726-155745	None:intergenic	45.0%
!	TACCTTTTTGCAAGCCAGAC+TGG	+	contig271end:157154-157173	MsG0080048313.01.T01:intron	45.0%
!	TAGTCCCTCCTCTAAGAGAT+TGG	+	contig271end:154379-154398	MsG0080048313.01.T01:intron	45.0%
!	TCTGGCTTGCAAAAAGGTAG+TGG	-	contig271end:157153-157172	None:intergenic	45.0%
!	TGGCGGGCATGTTATAGATA+GGG	-	contig271end:157337-157356	None:intergenic	45.0%
!!	GCTTCATTGGCTGTTTCTTC+AGG	+	contig271end:157111-157130	MsG0080048313.01.T01:intron	45.0%
!!	GCTTGGTTTGCCATTCTAGT+GGG	+	contig271end:156998-157017	MsG0080048313.01.T01:intron	45.0%
!!	GTGGTATTGGTTTGGGATCT+GGG	+	contig271end:157542-157561	MsG0080048313.01.T01:intron	45.0%
	AATGCTGTCATCTGGGGATG+GGG	+	contig271end:157891-157910	MsG0080048313.01.T01:CDS	50.0%
	AATTTCTTTGCAGCCAGGGC+AGG	-	contig271end:155756-155775	None:intergenic	50.0%
	AGAATCGAACCTGAGACTCG+AGG	-	contig271end:157579-157598	None:intergenic	50.0%
	AGCTCCCAATCTCTTAGAGG+AGG	-	contig271end:154387-154406	None:intergenic	50.0%
	AGTACTACTGCTAGAGCTGG+TGG	+	contig271end:154302-154321	MsG0080048313.01.T01:CDS	50.0%
	AGTGCTGTAGCTGCAATGCT+TGG	+	contig271end:156903-156922	MsG0080048313.01.T01:intron	50.0%
	ATCATTCTCCTTGAGCTGGC+CGG	+	contig271end:157089-157108	MsG0080048313.01.T01:intron	50.0%
	ATCCAAACAGGCCCTAAGTG+GGG	-	contig271end:156082-156101	None:intergenic	50.0%
	CAGCAAGTGGAAGGAGAGTT+CGG	-	contig271end:155453-155472	None:intergenic	50.0%
	CATCCAAACAGGCCCTAAGT+GGG	-	contig271end:156083-156102	None:intergenic	50.0%
	CATGTAGCCAACCCCACTTA+GGG	+	contig271end:156068-156087	MsG0080048313.01.T01:intron	50.0%
	CCTAGTACTACTGCTAGAGC+TGG	+	contig271end:154299-154318	MsG0080048313.01.T01:CDS	50.0%
	CTGCGTTTCTTGCCATGCAT+AGG	+	contig271end:157197-157216	MsG0080048313.01.T01:intron	50.0%
	GAGCTGGATGTCTGATTGTG+TGG	+	contig271end:157053-157072	MsG0080048313.01.T01:intron	50.0%
	GAGCTTATAGTTAGGGACGG+AGG	-	contig271end:156323-156342	None:intergenic	50.0%
	GATCCCAAACCAATACCACC+AGG	-	contig271end:157541-157560	None:intergenic	50.0%
	GCAGTAGTACTAGGCATAGC+AGG	-	contig271end:154293-154312	None:intergenic	50.0%
	GCCCTGTCACTCAAATGAGT+GGG	+	contig271end:155787-155806	MsG0080048313.01.T01:CDS	50.0%
	GCTCCCAATCTCTTAGAGGA+GGG	-	contig271end:154386-154405	None:intergenic	50.0%
	GGAGAATGATCGAAGGCTGT+TGG	-	contig271end:157079-157098	None:intergenic	50.0%
	GTCTCAGGTTCGATTCTCTC+CGG	+	contig271end:157582-157601	MsG0080048313.01.T01:intron	50.0%
	GTGCTTAAACCCAGTTGGGT+TGG	+	contig271end:157515-157534	MsG0080048313.01.T01:intron	50.0%
	TAGCCCACCCAAATTGACAC+CGG	-	contig271end:157604-157623	None:intergenic	50.0%
	TGCCCTGGCTGCAAAGAAAT+TGG	+	contig271end:155755-155774	MsG0080048313.01.T01:CDS	50.0%
	TGTCTTGCAGAGAGACTCCT+TGG	+	contig271end:156873-156892	MsG0080048313.01.T01:intron	50.0%
	TTCAAGCAGTCATCCCGTCA+AGG	-	contig271end:156954-156973	None:intergenic	50.0%
!	AGCAGTTAGGAAAAGAGCGC+TGG	-	contig271end:155561-155580	None:intergenic	50.0%
!	AGTCGATGCCTTTTCCCACT+TGG	-	contig271end:156632-156651	None:intergenic	50.0%
!	CCAGCTCTAGCAGTAGTACT+AGG	-	contig271end:154302-154321	None:intergenic	50.0%
!	GGCAAAAGCATACACCAACC+TGG	-	contig271end:155237-155256	None:intergenic	50.0%
!	TCTCCGGTGTCAATTTGGGT+GGG	+	contig271end:157598-157617	MsG0080048313.01.T01:intron	50.0%
!!	ATAAGGCTTAGTGGTGGTGG+TGG	+	contig271end:155319-155338	MsG0080048313.01.T01:intron	50.0%
!!	CCGTCAAGGACTCTAAGACA+AGG	-	contig271end:156940-156959	None:intergenic	50.0%
!!	CTACAGCACTTGCTATGCCA+AGG	-	contig271end:156893-156912	None:intergenic	50.0%
!!	GAAAAGAGCGCTGGAGTTAC+CGG	-	contig271end:155552-155571	None:intergenic	50.0%
!!	GGCTTGGTTTGCCATTCTAG+TGG	+	contig271end:156997-157016	MsG0080048313.01.T01:intron	50.0%
!!	GGTGGTATTGGTTTGGGATC+TGG	+	contig271end:157541-157560	MsG0080048313.01.T01:intron	50.0%
!!	GGTGGTGGTGGTTGTTGTAT+TGG	+	contig271end:155331-155350	MsG0080048313.01.T01:intron	50.0%
!!	GTGGCGGGCATGTTATAGAT+AGG	-	contig271end:157338-157357	None:intergenic	50.0%
!!	TGGCCTGGTGGTATTGGTTT+GGG	+	contig271end:157535-157554	MsG0080048313.01.T01:intron	50.0%
!!	TTGGCCTGGTGGTATTGGTT+TGG	+	contig271end:157534-157553	MsG0080048313.01.T01:intron	50.0%
	AAACAGGCCCTAAGTGGGGT+TGG	-	contig271end:156078-156097	None:intergenic	55.0%
	AACCCCACTTAGGGCCTGTT+TGG	+	contig271end:156077-156096	MsG0080048313.01.T01:intron	55.0%
	AAGCCAGACTGGGCATGTAG+GGG	+	contig271end:157165-157184	MsG0080048313.01.T01:intron	55.0%
	ACATCCAGCTCACAATCCCG+AGG	-	contig271end:157044-157063	None:intergenic	55.0%
	ACTCTCGCAGCGCTTGCTTT+AGG	+	contig271end:157238-157257	MsG0080048313.01.T01:intron	55.0%
	ATCGAACCTGAGACTCGAGG+AGG	-	contig271end:157576-157595	None:intergenic	55.0%
	CAAACCTCGGGATTGTGAGC+TGG	+	contig271end:157037-157056	MsG0080048313.01.T01:intron	55.0%
	CAAGCCAGACTGGGCATGTA+GGG	+	contig271end:157164-157183	MsG0080048313.01.T01:intron	55.0%
	CAGCATGGGATACCTTGGCT+TGG	+	contig271end:156981-157000	MsG0080048313.01.T01:intron	55.0%
	CCACTTAGGGCCTGTTTGGA+TGG	+	contig271end:156081-156100	MsG0080048313.01.T01:intron	55.0%
	CCATCCAAACAGGCCCTAAG+TGG	-	contig271end:156084-156103	None:intergenic	55.0%
	CCATGTAGCCAACCCCACTT+AGG	+	contig271end:156067-156086	MsG0080048313.01.T01:intron	55.0%
	GGCCCTGTCACTCAAATGAG+TGG	+	contig271end:155786-155805	MsG0080048313.01.T01:CDS	55.0%
	GGGATAAGGCTTAGTGGTGG+TGG	+	contig271end:155316-155335	MsG0080048313.01.T01:intron	55.0%
!	CTCTCCGGTGTCAATTTGGG+TGG	+	contig271end:157597-157616	MsG0080048313.01.T01:intron	55.0%
!	GCCCAGTCTGGCTTGCAAAA+AGG	-	contig271end:157159-157178	None:intergenic	55.0%
!!	CCTAAGTGGGGTTGGCTACA+TGG	-	contig271end:156070-156089	None:intergenic	55.0%
!!	GTGGGAAAAGGCATCGACTG+TGG	+	contig271end:156633-156652	MsG0080048313.01.T01:intron	55.0%
!!	TAAACCCAGTTGGGTTGGCC+TGG	+	contig271end:157520-157539	MsG0080048313.01.T01:intron	55.0%
!!	TTGGGTTGGCCTGGTGGTAT+TGG	+	contig271end:157529-157548	MsG0080048313.01.T01:intron	55.0%
	AAGCCCCTACATGCCCAGTC+TGG	-	contig271end:157171-157190	None:intergenic	60.0%
	ACCACCAGGCCAACCCAACT+GGG	-	contig271end:157527-157546	None:intergenic	60.0%
	ATGGGGTGGTGTGGCTCATC+TGG	+	contig271end:157908-157927	MsG0080048313.01.T01:CDS	60.0%
	CAATGAAGCCGGCCAGCTCA+AGG	-	contig271end:157100-157119	None:intergenic	60.0%
	CTTGCCATGCATAGGGCAGC+TGG	+	contig271end:157205-157224	MsG0080048313.01.T01:intron	60.0%
	GACACCAGCTGCCCTATGCA+TGG	-	contig271end:157212-157231	None:intergenic	60.0%
	GCAAGCCAGACTGGGCATGT+AGG	+	contig271end:157163-157182	MsG0080048313.01.T01:intron	60.0%
	TACCACCAGGCCAACCCAAC+TGG	-	contig271end:157528-157547	None:intergenic	60.0%
	TGTGCTCCTCCTCGAGTCTC+AGG	+	contig271end:157567-157586	MsG0080048313.01.T01:intron	60.0%
!	CTTGAGCTGGCCGGCTTCAT+TGG	+	contig271end:157098-157117	MsG0080048313.01.T01:intron	60.0%
!!	CAAGCGCTGCGAGAGTACCA+GGG	-	contig271end:157234-157253	None:intergenic	60.0%
	CATAGGGCAGCTGGTGTCCC+TGG	+	contig271end:157214-157233	MsG0080048313.01.T01:intron	65.0%
	CATCTGGGGATGGGGTGGTG+TGG	+	contig271end:157899-157918	MsG0080048313.01.T01:CDS	65.0%
	GCTGTCATCTGGGGATGGGG+TGG	+	contig271end:157894-157913	MsG0080048313.01.T01:CDS	65.0%
	GGGTGGTGTGGCTCATCTGG+TGG	+	contig271end:157911-157930	MsG0080048313.01.T01:CDS	65.0%
!!	ACCCAGTTGGGTTGGCCTGG+TGG	+	contig271end:157523-157542	MsG0080048313.01.T01:intron	65.0%
!!	GCAAGCGCTGCGAGAGTACC+AGG	-	contig271end:157235-157254	None:intergenic	65.0%
	GCAGCCAGGGCAGGTGCCTC+TGG	-	contig271end:155747-155766	None:intergenic	75.0%
!!	CACGCCAGAGGCACCTGCCC+TGG	+	contig271end:155740-155759	MsG0080048313.01.T01:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
contig271end	gene	154230	158211	154230	ID=MsG0080048313.01;Name=MsG0080048313.01
contig271end	mRNA	154230	158211	154230	ID=MsG0080048313.01.T01;Parent=MsG0080048313.01;Name=MsG0080048313.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0.33\|1\|0.5\|0.33\|3\|258\|149
contig271end	exon	157836	158211	157836	ID=MsG0080048313.01.T01:exon:2231;Parent=MsG0080048313.01.T01
contig271end	exon	155613	155808	155613	ID=MsG0080048313.01.T01:exon:2230;Parent=MsG0080048313.01.T01
contig271end	exon	154230	154365	154230	ID=MsG0080048313.01.T01:exon:2229;Parent=MsG0080048313.01.T01
contig271end	CDS	154230	154365	154230	ID=MsG0080048313.01.T01:cds;Parent=MsG0080048313.01.T01
contig271end	CDS	155613	155808	155613	ID=MsG0080048313.01.T01:cds;Parent=MsG0080048313.01.T01
contig271end	CDS	157836	157953	157836	ID=MsG0080048313.01.T01:cds;Parent=MsG0080048313.01.T01
contig271end	three_prime_UTR	157954	158211	157954	ID=MsG0080048313.01.T01:three_prime_utr;Parent=MsG0080048313.01.T01

Gene Sequence

>MsG0080048313.01.T01

ATGGGGAAGAGTACTTTAGGACTTTCTTATGGTTTGACATTTAGTGAAGTTCTTATTGCACCTGCTATGCCTAGTACTACTGCTAGAGCTGGTGGTGTTTTTTTGCCTATTATTAAGTCACTTTCACTCTCTGCTGCACAGAGCTTGGGGTGTAATTTCAAACCCTTGGGTTACGTGGTTAAAGCAGCTAGTGTACCTGCATTTGTTTGTCTTCTTGCTACACCGTTGATTTTATACACGATCTATCCTCCTGAAATTAAAGACACGCCAGAGGCACCTGCCCTGGCTGCAAAGAAATTGGAAACAATGGGCCCTGTCACTCAAATGAGTGGCTGGCTATATAGACCTTCCCGATATATTCAAACGGGTTTTATATATGGCATTTTTAATGCTGTCATCTGGGGATGGGGTGGTGTGGCTCATCTGGTGGAAATTTTTGGCTTGTATTAA

Protein sequence

>MsG0080048313.01.T01

MGKSTLGLSYGLTFSEVLIAPAMPSTTARAGGVFLPIIKSLSLSAAQSLGCNFKPLGYVVKAASVPAFVCLLATPLILYTIYPPEIKDTPEAPALAAKKLETMGPVTQMSGWLYRPSRYIQTGFIYGIFNAVIWGWGGVAHLVEIFGLY*