Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048560.01.T01 | VDD65938.1 | 92.079 | 101 | 7 | 1 | 1 | 100 | 13 | 113 | 3.89E-58 | 185 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048560.01.T01 | A0A3P6GHH9 | 92.079 | 101 | 7 | 1 | 1 | 100 | 13 | 113 | 1.86e-58 | 185 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0080048560.01 | -0.800180 | 1.626259e-48 | 7.522787e-46 |
| MsG0080047967.01 | MsG0080048560.01 | 0.833863 | 4.176774e-56 | 4.872103e-53 |
| MsG0080048059.01 | MsG0080048560.01 | 0.852153 | 5.565230e-61 | 1.152385e-57 |
| MsG0080048560.01 | MsG0080048689.01 | 0.807471 | 4.974894e-50 | 2.773386e-47 |
| MsG0080048560.01 | MsG0080048690.01 | 0.861235 | 1.183088e-63 | 3.324287e-60 |
| MsG0080048560.01 | MsG0080048691.01 | 0.852159 | 5.541277e-61 | 1.147667e-57 |
| MsG0080048560.01 | MsG0080048692.01 | 0.845930 | 2.985598e-59 | 5.055548e-56 |
| MsG0080048560.01 | MsG0080048693.01 | 0.800650 | 1.304526e-48 | 6.107138e-46 |
| MsG0080048560.01 | MsG0080048694.01 | 0.866926 | 1.993739e-65 | 6.836953e-62 |
| MsG0080048560.01 | MsG0080048695.01 | 0.867520 | 1.288004e-65 | 4.509692e-62 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048560.01.T01 | MTR_4g034790 | 95.161 | 62 | 3 | 0 | 31 | 92 | 134 | 195 | 1.07e-33 | 117 |
| MsG0080048560.01.T01 | MTR_0002s0750 | 96.667 | 60 | 2 | 0 | 33 | 92 | 148 | 207 | 4.59e-33 | 115 |
Find 34 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATCGTTAGGCCACGC+GGG | 0.590659 | contig356end:-16902 | MsG0080048560.01.T01:intergenic |
| AAAATATATAAAAGATCGTT+AGG | 0.468270 | contig356end:-16911 | None:intergenic |
| AACCGCTAAGCCTACGTTAC+AGG | 0.327005 | contig356end:-16960 | MsG0080048560.01.T01:intergenic |
| AAGAACGCCTAATAATTTAT+TGG | 0.207304 | contig356end:-17089 | None:intergenic |
| AAGGGATGGGTCATAATTTA+TGG | 0.230017 | contig356end:+16873 | None:intergenic |
| ACAGTACCCAATAAATTATT+AGG | 0.414832 | contig356end:+17082 | MsG0080048560.01.T01:CDS |
| AGAACGCCTAATAATTTATT+GGG | 0.334901 | contig356end:-17088 | MsG0080048560.01.T01:intergenic |
| AGGAGTTGCAAACGGATCTG+CGG | 0.603433 | contig356end:-17008 | MsG0080048560.01.T01:intergenic |
| AGGGATGGGTCATAATTTAT+GGG | 0.496639 | contig356end:+16874 | None:intergenic |
| AGGTACTGTTAATAATCCCA+CGG | 0.598380 | contig356end:-17128 | MsG0080048560.01.T01:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAGTACCTAGAATTACTAC+TGG | - | contig356end:16938-16957 | None:intergenic | 30.0% | |
| TACGGGAAAAAAATACCATT+CGG | - | contig356end:17047-17066 | MsG0080048560.01.T01:intergenic | 30.0% | |
| TTCTTGAACCATCAATGATT+GGG | + | contig356end:16981-17000 | MsG0080048560.01.T01:CDS | 30.0% | |
| ACGGGAAAAAAATACCATTC+GGG | - | contig356end:17046-17065 | None:intergenic | 35.0% | |
| ATTTGCAAAGGAGTTGCAAA+CGG | - | contig356end:17019-17038 | MsG0080048560.01.T01:intergenic | 35.0% | |
| GTTCTTGAACCATCAATGAT+TGG | + | contig356end:16980-16999 | MsG0080048560.01.T01:CDS | 35.0% | |
| TCTTGAACCATCAATGATTG+GGG | + | contig356end:16982-17001 | MsG0080048560.01.T01:CDS | 35.0% | |
| CATTCGGGCAATATTTGCAA+AGG | - | contig356end:17031-17050 | MsG0080048560.01.T01:intergenic | 40.0% | |
| CTTTGCAAATATTGCCCGAA+TGG | + | contig356end:17029-17048 | MsG0080048560.01.T01:CDS | 40.0% | |
| GGTACTGTTAATAATCCCAC+GGG | - | contig356end:17130-17149 | MsG0080048560.01.T01:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig356end | gene | 16878 | 17192 | 16878 | ID=MsG0080048560.01;Name=MsG0080048560.01 |
| contig356end | mRNA | 16878 | 17192 | 16878 | ID=MsG0080048560.01.T01;Parent=MsG0080048560.01;Name=MsG0080048560.01.T01;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|0|1|0|104 |
| contig356end | exon | 16878 | 17192 | 16878 | ID=MsG0080048560.01.T01:exon:17605;Parent=MsG0080048560.01.T01 |
| contig356end | CDS | 16878 | 17192 | 16878 | ID=MsG0080048560.01.T01:cds;Parent=MsG0080048560.01.T01 |
| Gene Sequence |
>MsG0080048560.01.T01 ATGGGTCATAATTTATGGGGAGAACCCGCGTGGCCTAACGATCTTTTATATATTTTTCCAGTAGTAATTCTAGGTACTATTGCCTGTAACGTAGGCTTAGCGGTTCTTGAACCATCAATGATTGGGGAACCCGCAGATCCGTTTGCAACTCCTTTGCAAATATTGCCCGAATGGTATTTTTTTCCCGTATTTCAAATACTTCGTACAGTACCCAATAAATTATTAGGCGTTCTTTTAATGGTTTCAGTACCCGTGGGATTATTAACAGTACCTTTTTGGAGAATGTTAATAAGTTCCAAACCCTTGTATTTCTAG |
| Protein sequence |
>MsG0080048560.01.T01 MGHNLWGEPAWPNDLLYIFPVVILGTIACNVGLAVLEPSMIGEPADPFATPLQILPEWYFFPVFQILRTVPNKLLGVLLMVSVPVGLLTVPFWRMLISSKPLYF* |
