Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014293.01.T01 | XP_003600177.1 | 91.667 | 156 | 2 | 1 | 1 | 145 | 1 | 156 | 8.80E-95 | 280 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014293.01.T01 | G7J032 | 91.667 | 156 | 2 | 1 | 1 | 145 | 1 | 156 | 1.10E-97 | 280 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014293.01.T01 | A0A396INQ3 | 91.667 | 156 | 2 | 1 | 1 | 145 | 1 | 156 | 4.21e-95 | 280 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001503.01 | MsG0380014293.01 | 0.803810 | 2.918566e-49 | 1.480713e-46 |
| MsG0380014293.01 | MsG0380014646.01 | -0.810774 | 9.753137e-51 | 5.932153e-48 |
| MsG0380014293.01 | MsG0480021133.01 | 0.816274 | 6.018716e-52 | 4.245825e-49 |
| MsG0380014293.01 | MsG0480022298.01 | 0.805903 | 1.066370e-49 | 5.709475e-47 |
| MsG0380014293.01 | MsG0680034974.01 | 0.841226 | 5.401923e-58 | 7.884357e-55 |
| MsG0380014293.01 | MsG0780036208.01 | 0.810050 | 1.397645e-50 | 8.339644e-48 |
| MsG0380014293.01 | MsG0780038519.01 | 0.809616 | 1.732478e-50 | 1.022121e-47 |
| MsG0380014293.01 | MsG0880045851.01 | 0.830910 | 2.249836e-55 | 2.403737e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014293.01.T01 | MTR_3g055120 | 91.667 | 156 | 2 | 1 | 1 | 145 | 1 | 156 | 1.07e-98 | 280 |
| MsG0380014293.01.T01 | MTR_3g055120 | 92.208 | 154 | 3 | 2 | 1 | 145 | 1 | 154 | 1.29e-96 | 275 |
| MsG0380014293.01.T01 | MTR_3g055130 | 57.692 | 156 | 54 | 2 | 1 | 145 | 1 | 155 | 1.87e-54 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACATCTTTCACAATATT+TGG | 0.180335 | 3:+52534704 | None:intergenic |
| CCCTACATTTAGTGTTGTTT+GGG | 0.266669 | 3:+52534787 | None:intergenic |
| ACCCTACATTTAGTGTTGTT+TGG | 0.267738 | 3:+52534786 | None:intergenic |
| ACTATGTAAACATTGAAATA+AGG | 0.268914 | 3:-52534087 | MsG0380014293.01.T01:three_prime_UTR |
| ACCTCCCTTTGATAATTTAC+TGG | 0.269590 | 3:+52534418 | None:intergenic |
| ATGTAGGGTTGGAGGCTCTC+TGG | 0.280146 | 3:-52534772 | MsG0380014293.01.T01:CDS |
| GCAATCTTCAACAAATAAAA+TGG | 0.299028 | 3:+52534113 | None:intergenic |
| TCTCTGATATTGCTGAGAAT+TGG | 0.334808 | 3:+52534299 | None:intergenic |
| ACAATATTTGGTAGAACCTT+TGG | 0.336066 | 3:+52534716 | None:intergenic |
| GATGAGTTATCCCATACAAT+TGG | 0.363483 | 3:-52534382 | MsG0380014293.01.T01:CDS |
| CCACTTGGAGCCCAATTGTA+TGG | 0.392253 | 3:+52534371 | None:intergenic |
| ATTCTCATATCAATTGCTAA+TGG | 0.407429 | 3:+52534050 | None:intergenic |
| TTGCTAATGGGTGTTGCTCT+TGG | 0.424252 | 3:+52534063 | None:intergenic |
| TTCTCATATCAATTGCTAAT+GGG | 0.425052 | 3:+52534051 | None:intergenic |
| TGTAGGGTTGGAGGCTCTCT+GGG | 0.431104 | 3:-52534771 | MsG0380014293.01.T01:CDS |
| AGTAATTGAAGGGGATGGAG+GGG | 0.458591 | 3:-52534681 | MsG0380014293.01.T01:CDS |
| AATTGATATGAGAATCCCTT+TGG | 0.461217 | 3:-52534042 | MsG0380014293.01.T01:three_prime_UTR |
| ACATTGATGAGTCATGATAA+AGG | 0.485079 | 3:-52534819 | None:intergenic |
| AAGTAATTGAAGGGGATGGA+GGG | 0.491464 | 3:-52534682 | MsG0380014293.01.T01:CDS |
| AATATCAGAGAATAAAACTT+TGG | 0.496609 | 3:-52534287 | MsG0380014293.01.T01:CDS |
| GATAGCCTCCTTCAACCACT+TGG | 0.497730 | 3:+52534356 | None:intergenic |
| ATGAGTTATCCCATACAATT+GGG | 0.498688 | 3:-52534381 | MsG0380014293.01.T01:CDS |
| CAAGTAATTGAAGGGGATGG+AGG | 0.503953 | 3:-52534683 | MsG0380014293.01.T01:CDS |
| TAGAACCTTTGGAACAACTA+AGG | 0.509620 | 3:+52534727 | None:intergenic |
| TTGCCCAAGATAAAATAAAG+TGG | 0.515677 | 3:+52534189 | None:intergenic |
| AACACTAAATGTAGGGTTGG+AGG | 0.516628 | 3:-52534780 | MsG0380014293.01.T01:CDS |
| GTAATTGAAGGGGATGGAGG+GGG | 0.517235 | 3:-52534680 | MsG0380014293.01.T01:intron |
| ATTGGGCTCCAAGTGGTTGA+AGG | 0.523194 | 3:-52534364 | MsG0380014293.01.T01:CDS |
| CCCAAACAACACTAAATGTA+GGG | 0.526439 | 3:-52534787 | MsG0380014293.01.T01:CDS |
| AACAACTAAGGTGATATCCT+TGG | 0.527317 | 3:+52534739 | None:intergenic |
| TATCTCCAGTAAATTATCAA+AGG | 0.528165 | 3:-52534423 | MsG0380014293.01.T01:CDS |
| GAGATCAAGTTAAGGCCAAA+GGG | 0.530989 | 3:+52534027 | None:intergenic |
| ATCTCCAGTAAATTATCAAA+GGG | 0.531150 | 3:-52534422 | MsG0380014293.01.T01:CDS |
| TATCACCTTAGTTGTTCCAA+AGG | 0.536797 | 3:-52534732 | MsG0380014293.01.T01:CDS |
| CACTTGGAGCCCAATTGTAT+GGG | 0.538605 | 3:+52534372 | None:intergenic |
| ACCCAAACAACACTAAATGT+AGG | 0.541086 | 3:-52534788 | MsG0380014293.01.T01:CDS |
| GTGGACTCTGATGTCTTCGT+AGG | 0.554921 | 3:+52534208 | None:intergenic |
| AACAACACTAAATGTAGGGT+TGG | 0.556486 | 3:-52534783 | MsG0380014293.01.T01:CDS |
| AGAGATCAAGTTAAGGCCAA+AGG | 0.556516 | 3:+52534026 | None:intergenic |
| AAAGATGTGCAAGTAATTGA+AGG | 0.557421 | 3:-52534692 | MsG0380014293.01.T01:CDS |
| GAAGGAGGCTATCTCAATCA+AGG | 0.560280 | 3:-52534346 | MsG0380014293.01.T01:CDS |
| GTGCAAGTAATTGAAGGGGA+TGG | 0.570134 | 3:-52534686 | MsG0380014293.01.T01:CDS |
| TCTCTGGGCTGCACAGTCCA+AGG | 0.592809 | 3:-52534756 | MsG0380014293.01.T01:CDS |
| AAGATGTGCAAGTAATTGAA+GGG | 0.598044 | 3:-52534691 | MsG0380014293.01.T01:CDS |
| TCCAGTAAATTATCAAAGGG+AGG | 0.626622 | 3:-52534419 | MsG0380014293.01.T01:CDS |
| GGGCTCCAAGTGGTTGAAGG+AGG | 0.646381 | 3:-52534361 | MsG0380014293.01.T01:CDS |
| CCATACAATTGGGCTCCAAG+TGG | 0.700462 | 3:-52534371 | MsG0380014293.01.T01:CDS |
| AGATGTGCAAGTAATTGAAG+GGG | 0.714548 | 3:-52534690 | MsG0380014293.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATCAAATTATTATATCTT+AGG | - | Chr3:52534398-52534417 | MsG0380014293.01.T01:CDS | 10.0% |
| !! | AATATCAGAGAATAAAACTT+TGG | - | Chr3:52534559-52534578 | MsG0380014293.01.T01:intron | 20.0% |
| !! | ACTATGTAAACATTGAAATA+AGG | - | Chr3:52534759-52534778 | MsG0380014293.01.T01:CDS | 20.0% |
| !! | CAAAAAATTAAAGATGAGTT+TGG | + | Chr3:52534198-52534217 | None:intergenic | 20.0% |
| !! | CTAGAAAAATTTCTGTTAAA+TGG | - | Chr3:52534680-52534699 | MsG0380014293.01.T01:intron | 20.0% |
| ! | ATCTCCAGTAAATTATCAAA+GGG | - | Chr3:52534424-52534443 | MsG0380014293.01.T01:CDS | 25.0% |
| ! | ATTCTCATATCAATTGCTAA+TGG | + | Chr3:52534799-52534818 | None:intergenic | 25.0% |
| ! | GCAATCTTCAACAAATAAAA+TGG | + | Chr3:52534736-52534755 | None:intergenic | 25.0% |
| ! | TATCTCCAGTAAATTATCAA+AGG | - | Chr3:52534423-52534442 | MsG0380014293.01.T01:CDS | 25.0% |
| ! | TTCTCATATCAATTGCTAAT+GGG | + | Chr3:52534798-52534817 | None:intergenic | 25.0% |
| !!! | AGTCCACTTTATTTTATCTT+GGG | - | Chr3:52534654-52534673 | MsG0380014293.01.T01:intron | 25.0% |
| AAAGATGTGCAAGTAATTGA+AGG | - | Chr3:52534154-52534173 | MsG0380014293.01.T01:exon | 30.0% | |
| AAGATGTGCAAGTAATTGAA+GGG | - | Chr3:52534155-52534174 | MsG0380014293.01.T01:exon | 30.0% | |
| AATTGATATGAGAATCCCTT+TGG | - | Chr3:52534804-52534823 | MsG0380014293.01.T01:CDS | 30.0% | |
| ACAATATTTGGTAGAACCTT+TGG | + | Chr3:52534133-52534152 | None:intergenic | 30.0% | |
| ATGAGTTATCCCATACAATT+GGG | - | Chr3:52534465-52534484 | MsG0380014293.01.T01:intron | 30.0% | |
| TGCACATCTTTCACAATATT+TGG | + | Chr3:52534145-52534164 | None:intergenic | 30.0% | |
| TTGCCCAAGATAAAATAAAG+TGG | + | Chr3:52534660-52534679 | None:intergenic | 30.0% | |
| ! | ACTATTTTACAACGTGTTTC+AGG | + | Chr3:52534297-52534316 | None:intergenic | 30.0% |
| ! | CTATTTTACAACGTGTTTCA+GGG | + | Chr3:52534296-52534315 | None:intergenic | 30.0% |
| !! | GTTTGACTTTTTCTTCAATG+AGG | + | Chr3:52534619-52534638 | None:intergenic | 30.0% |
| !!! | CTCATCTTTAATTTTTTGCC+TGG | - | Chr3:52534200-52534219 | MsG0380014293.01.T01:CDS | 30.0% |
| !!! | GAGTCCACTTTATTTTATCT+TGG | - | Chr3:52534653-52534672 | MsG0380014293.01.T01:intron | 30.0% |
| AACAACACTAAATGTAGGGT+TGG | - | Chr3:52534063-52534082 | MsG0380014293.01.T01:three_prime_UTR | 35.0% | |
| AACAACTAAGGTGATATCCT+TGG | + | Chr3:52534110-52534129 | None:intergenic | 35.0% | |
| ACCCAAACAACACTAAATGT+AGG | - | Chr3:52534058-52534077 | MsG0380014293.01.T01:three_prime_UTR | 35.0% | |
| AGATGTGCAAGTAATTGAAG+GGG | - | Chr3:52534156-52534175 | MsG0380014293.01.T01:exon | 35.0% | |
| ATGCAATCAAAATCTCGATG+AGG | + | Chr3:52534256-52534275 | None:intergenic | 35.0% | |
| CCCAAACAACACTAAATGTA+GGG | - | Chr3:52534059-52534078 | MsG0380014293.01.T01:three_prime_UTR | 35.0% | |
| GATGAGTTATCCCATACAAT+TGG | - | Chr3:52534464-52534483 | MsG0380014293.01.T01:intron | 35.0% | |
| TATCACCTTAGTTGTTCCAA+AGG | - | Chr3:52534114-52534133 | MsG0380014293.01.T01:three_prime_UTR | 35.0% | |
| TCCAGTAAATTATCAAAGGG+AGG | - | Chr3:52534427-52534446 | MsG0380014293.01.T01:CDS | 35.0% | |
| TCTCTGATATTGCTGAGAAT+TGG | + | Chr3:52534550-52534569 | None:intergenic | 35.0% | |
| TGCAATCAAAATCTCGATGA+GGG | + | Chr3:52534255-52534274 | None:intergenic | 35.0% | |
| ! | ACCCTACATTTAGTGTTGTT+TGG | + | Chr3:52534063-52534082 | None:intergenic | 35.0% |
| ! | ACCTCCCTTTGATAATTTAC+TGG | + | Chr3:52534431-52534450 | None:intergenic | 35.0% |
| ! | GGTGTTGAATTCCTTTATCA+TGG | + | Chr3:52534041-52534060 | None:intergenic | 35.0% |
| !! | CCCTACATTTAGTGTTGTTT+GGG | + | Chr3:52534062-52534081 | None:intergenic | 35.0% |
| !! | TAGAACCTTTGGAACAACTA+AGG | + | Chr3:52534122-52534141 | None:intergenic | 35.0% |
| !!! | CATCGAGATTTTGATTGCAT+CGG | - | Chr3:52534256-52534275 | MsG0380014293.01.T01:CDS | 35.0% |
| AACACTAAATGTAGGGTTGG+AGG | - | Chr3:52534066-52534085 | MsG0380014293.01.T01:three_prime_UTR | 40.0% | |
| AAGTAATTGAAGGGGATGGA+GGG | - | Chr3:52534164-52534183 | MsG0380014293.01.T01:CDS | 40.0% | |
| TAAGTGTGCCTTCTCTCTTT+AGG | - | Chr3:52534223-52534242 | MsG0380014293.01.T01:CDS | 40.0% | |
| AGTAATTGAAGGGGATGGAG+GGG | - | Chr3:52534165-52534184 | MsG0380014293.01.T01:CDS | 45.0% | |
| CAAGTAATTGAAGGGGATGG+AGG | - | Chr3:52534163-52534182 | MsG0380014293.01.T01:CDS | 45.0% | |
| CACTTGGAGCCCAATTGTAT+GGG | + | Chr3:52534477-52534496 | None:intergenic | 45.0% | |
| GAAGGAGGCTATCTCAATCA+AGG | - | Chr3:52534500-52534519 | MsG0380014293.01.T01:intron | 45.0% | |
| GTGCAAGTAATTGAAGGGGA+TGG | - | Chr3:52534160-52534179 | MsG0380014293.01.T01:CDS | 45.0% | |
| TGCCTTCTCTCTTTAGGTCA+TGG | - | Chr3:52534229-52534248 | MsG0380014293.01.T01:CDS | 45.0% | |
| !! | TTGCTAATGGGTGTTGCTCT+TGG | + | Chr3:52534786-52534805 | None:intergenic | 45.0% |
| ATTGGGCTCCAAGTGGTTGA+AGG | - | Chr3:52534482-52534501 | MsG0380014293.01.T01:intron | 50.0% | |
| CCACTTGGAGCCCAATTGTA+TGG | + | Chr3:52534478-52534497 | None:intergenic | 50.0% | |
| CCATACAATTGGGCTCCAAG+TGG | - | Chr3:52534475-52534494 | MsG0380014293.01.T01:intron | 50.0% | |
| GATAGCCTCCTTCAACCACT+TGG | + | Chr3:52534493-52534512 | None:intergenic | 50.0% | |
| GGCCATGACCTAAAGAGAGA+AGG | + | Chr3:52534234-52534253 | None:intergenic | 50.0% | |
| GTAATTGAAGGGGATGGAGG+GGG | - | Chr3:52534166-52534185 | MsG0380014293.01.T01:CDS | 50.0% | |
| GTGGACTCTGATGTCTTCGT+AGG | + | Chr3:52534641-52534660 | None:intergenic | 50.0% | |
| ! | AGAGAGAAGGCACACTTACC+AGG | + | Chr3:52534221-52534240 | None:intergenic | 50.0% |
| ATGTAGGGTTGGAGGCTCTC+TGG | - | Chr3:52534074-52534093 | MsG0380014293.01.T01:three_prime_UTR | 55.0% | |
| TGTAGGGTTGGAGGCTCTCT+GGG | - | Chr3:52534075-52534094 | MsG0380014293.01.T01:three_prime_UTR | 55.0% | |
| GGGCTCCAAGTGGTTGAAGG+AGG | - | Chr3:52534485-52534504 | MsG0380014293.01.T01:intron | 60.0% | |
| TCTCTGGGCTGCACAGTCCA+AGG | - | Chr3:52534090-52534109 | MsG0380014293.01.T01:three_prime_UTR | 60.0% | |
| TTGAAGGGGATGGAGGGGGT+TGG | - | Chr3:52534170-52534189 | MsG0380014293.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 52534040 | 52534828 | 52534040 | ID=MsG0380014293.01;Name=MsG0380014293.01 |
| Chr3 | mRNA | 52534040 | 52534828 | 52534040 | ID=MsG0380014293.01.T01;Parent=MsG0380014293.01;Name=MsG0380014293.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0.5|1|1|1|2|119|145 |
| Chr3 | exon | 52534040 | 52534448 | 52534040 | ID=MsG0380014293.01.T01:exon:10342;Parent=MsG0380014293.01.T01 |
| Chr3 | exon | 52534681 | 52534828 | 52534681 | ID=MsG0380014293.01.T01:exon:10341;Parent=MsG0380014293.01.T01 |
| Chr3 | CDS | 52534681 | 52534828 | 52534681 | ID=MsG0380014293.01.T01:cds;Parent=MsG0380014293.01.T01 |
| Chr3 | CDS | 52534159 | 52534448 | 52534159 | ID=MsG0380014293.01.T01:cds;Parent=MsG0380014293.01.T01 |
| Chr3 | three_prime_UTR | 52534040 | 52534158 | 52534040 | ID=MsG0380014293.01.T01:three_prime_utr;Parent=MsG0380014293.01.T01 |
| Gene Sequence |
| Protein sequence |
