Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014467.01.T01 | KEH34026.1 | 98.81 | 336 | 4 | 0 | 1 | 336 | 1 | 336 | 0 | 685 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014467.01.T01 | Q39218 | 65.217 | 345 | 102 | 4 | 1 | 336 | 56 | 391 | 8.52E-159 | 452 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014467.01.T01 | A0A072UX64 | 98.810 | 336 | 4 | 0 | 1 | 336 | 1 | 336 | 0.0 | 685 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048505.01 | MsG0380014467.01 | 0.800138 | 1.658614e-48 | 7.664512e-46 |
| MsG0180005339.01 | MsG0380014467.01 | 0.800914 | 1.152676e-48 | 5.432580e-46 |
| MsG0380014467.01 | MsG0480021690.01 | 0.829441 | 5.140203e-55 | 5.261095e-52 |
| MsG0380014467.01 | MsG0480023246.01 | 0.827102 | 1.883003e-54 | 1.800058e-51 |
| MsG0380014467.01 | MsG0880044099.01 | 0.804895 | 1.734213e-49 | 9.048696e-47 |
| MsG0380014467.01 | MsG0880047614.01 | 0.834558 | 2.796682e-56 | 3.331034e-53 |
| MsG0280008098.01 | MsG0380014467.01 | 0.803587 | 3.247305e-49 | 1.638168e-46 |
| MsG0280008419.01 | MsG0380014467.01 | 0.815143 | 1.075141e-51 | 7.352430e-49 |
| MsG0280009507.01 | MsG0380014467.01 | 0.802914 | 4.474864e-49 | 2.218773e-46 |
| MsG0280009757.01 | MsG0380014467.01 | 0.804209 | 2.411029e-49 | 1.235801e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014467.01.T01 | MTR_3g058410 | 98.810 | 336 | 4 | 0 | 1 | 336 | 1 | 336 | 0.0 | 685 |
| MsG0380014467.01.T01 | MTR_5g095470 | 71.111 | 315 | 81 | 3 | 22 | 336 | 17 | 321 | 4.57e-158 | 445 |
| MsG0380014467.01.T01 | MTR_8g028040 | 68.165 | 267 | 85 | 0 | 70 | 336 | 32 | 298 | 2.18e-134 | 384 |
| MsG0380014467.01.T01 | MTR_5g023760 | 54.118 | 255 | 112 | 1 | 70 | 324 | 55 | 304 | 2.02e-96 | 288 |
| MsG0380014467.01.T01 | MTR_5g023760 | 54.118 | 255 | 112 | 1 | 70 | 324 | 55 | 304 | 2.31e-96 | 289 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014467.01.T01 | AT3G13110 | 65.217 | 345 | 102 | 4 | 1 | 336 | 56 | 391 | 8.69e-160 | 452 |
| MsG0380014467.01.T01 | AT1G55920 | 65.875 | 337 | 91 | 5 | 1 | 336 | 1 | 314 | 4.27e-154 | 435 |
| MsG0380014467.01.T01 | AT5G56760 | 66.045 | 268 | 90 | 1 | 70 | 336 | 45 | 312 | 8.85e-128 | 368 |
| MsG0380014467.01.T01 | AT4G35640 | 59.843 | 254 | 97 | 1 | 71 | 324 | 74 | 322 | 3.48e-97 | 292 |
| MsG0380014467.01.T01 | AT2G17640 | 56.078 | 255 | 107 | 1 | 70 | 324 | 36 | 285 | 4.12e-92 | 278 |
Find 57 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCACTCTTGATTCAAAAT+AGG | 0.224483 | 3:-55236673 | MsG0380014467.01.T01:CDS |
| ATGGTGTTGAGGTTGATTTC+TGG | 0.253311 | 3:-55237048 | MsG0380014467.01.T01:CDS |
| ATTCATGATAAGGTTGAAAA+TGG | 0.304808 | 3:-55237067 | MsG0380014467.01.T01:CDS |
| TAAGATTCCTAGCTTTACTA+TGG | 0.312301 | 3:-55236294 | MsG0380014467.01.T01:CDS |
| AGCACTCTTGATTCAAAATA+GGG | 0.312531 | 3:-55236672 | MsG0380014467.01.T01:CDS |
| TATTCATGAAAAGATCAAAA+AGG | 0.323626 | 3:+55236870 | None:intergenic |
| ATAGGGTAGCACATAAATTA+TGG | 0.330521 | 3:-55236715 | MsG0380014467.01.T01:CDS |
| TGCTGGTTCTGTTGTGTTGA+AGG | 0.345531 | 3:-55236393 | MsG0380014467.01.T01:CDS |
| AAATAATAGCTAGATAAAAT+TGG | 0.349783 | 3:+55236983 | None:intergenic |
| AAAACACCATCACCAATCTT+AGG | 0.354890 | 3:+55236476 | None:intergenic |
| TTTCCAACAGCAGTAGTTCT+TGG | 0.373815 | 3:+55236362 | None:intergenic |
| ACATGTTCATATTCATGATA+AGG | 0.393517 | 3:-55237077 | MsG0380014467.01.T01:CDS |
| TCATGTTGATGAGGAATGTT+TGG | 0.409626 | 3:+55237193 | None:intergenic |
| CATTGTTTGTTGAATTTCAA+AGG | 0.415772 | 3:-55236758 | MsG0380014467.01.T01:CDS |
| TTTCCACCAATCAACTTAGC+TGG | 0.422664 | 3:+55236338 | None:intergenic |
| AATGTTACATTAGGTGGAAC+TGG | 0.429863 | 3:-55236521 | MsG0380014467.01.T01:CDS |
| GGAATCTTATCCAACATGAT+AGG | 0.441623 | 3:+55236308 | None:intergenic |
| ACTCAGAAATATATGAAGTA+TGG | 0.442129 | 3:+55236270 | None:intergenic |
| TTGCATAATGTTACATTAGG+TGG | 0.465580 | 3:-55236527 | MsG0380014467.01.T01:CDS |
| AATTTGACAAAATTGAATGA+AGG | 0.466844 | 3:+55237166 | None:intergenic |
| GGGAATATTAAGATTGGTGA+TGG | 0.475849 | 3:-55236428 | MsG0380014467.01.T01:CDS |
| ATTTGACAAAATTGAATGAA+GGG | 0.478931 | 3:+55237167 | None:intergenic |
| TTTGCTGTTGATATTCATCC+TGG | 0.479478 | 3:-55236638 | MsG0380014467.01.T01:CDS |
| ATGTTTGGTTTGCGAGAAGG+AGG | 0.480198 | 3:+55237208 | None:intergenic |
| ATTCATCCTGGTGCTAAGAT+TGG | 0.483510 | 3:-55236626 | MsG0380014467.01.T01:CDS |
| TTTGGTTTGCGAGAAGGAGG+AGG | 0.492117 | 3:+55237211 | None:intergenic |
| GGTGGAACTGGTAAGGTTTG+TGG | 0.495507 | 3:-55236509 | MsG0380014467.01.T01:CDS |
| CATGATTATGTGAAAAGGGT+CGG | 0.498203 | 3:+55237140 | None:intergenic |
| TTTAGCTTGTCAAGCTCATA+GGG | 0.501441 | 3:-55236732 | MsG0380014467.01.T01:CDS |
| TACATTAGGTGGAACTGGTA+AGG | 0.504651 | 3:-55236516 | MsG0380014467.01.T01:CDS |
| CCAACAGCAGTAGTTCTTGG+TGG | 0.511345 | 3:+55236365 | None:intergenic |
| TTGGTTTGCGAGAAGGAGGA+GGG | 0.517344 | 3:+55237212 | None:intergenic |
| GGAAATCCAGCTAAGTTGAT+TGG | 0.518731 | 3:-55236344 | MsG0380014467.01.T01:CDS |
| TGAACATAGCTTATACAAGC+AGG | 0.523004 | 3:+55236779 | None:intergenic |
| CCTGGTGCTAAGATTGGTAG+AGG | 0.530740 | 3:-55236620 | MsG0380014467.01.T01:CDS |
| GATGGTGCTAAGATTGGTGC+TGG | 0.531503 | 3:-55236410 | MsG0380014467.01.T01:CDS |
| CCACCAAGAACTACTGCTGT+TGG | 0.532511 | 3:-55236365 | MsG0380014467.01.T01:CDS |
| GATTCAAAATAGGGTTTCTG+AGG | 0.537609 | 3:-55236663 | MsG0380014467.01.T01:CDS |
| CATGCAACAGGAGTTGTTGT+TGG | 0.540340 | 3:-55236584 | MsG0380014467.01.T01:CDS |
| TGAAAATTCAAGAAGAAGCA+AGG | 0.541793 | 3:-55237024 | MsG0380014467.01.T01:CDS |
| CCTCTACCAATCTTAGCACC+AGG | 0.543694 | 3:+55236620 | None:intergenic |
| GGAATGTTTGGTTTGCGAGA+AGG | 0.546386 | 3:+55237205 | None:intergenic |
| ATTGGTGATGGTGCTAAGAT+TGG | 0.547373 | 3:-55236416 | MsG0380014467.01.T01:CDS |
| AAAAGATAATCCTATCATGT+TGG | 0.560483 | 3:-55236318 | MsG0380014467.01.T01:CDS |
| GTATGGTCCATAGTAAAGCT+AGG | 0.573050 | 3:+55236287 | None:intergenic |
| AATCCAGCTAAGTTGATTGG+TGG | 0.576016 | 3:-55236341 | MsG0380014467.01.T01:CDS |
| GGTGATAGACATCCTAAGAT+TGG | 0.586606 | 3:-55236488 | MsG0380014467.01.T01:CDS |
| GTTGGTGAAACTGCTGTGGT+TGG | 0.586963 | 3:-55236566 | MsG0380014467.01.T01:CDS |
| GCACATAAATTATGGCTACA+AGG | 0.604035 | 3:-55236707 | MsG0380014467.01.T01:CDS |
| GAAGGGTCATCATGTTGATG+AGG | 0.610233 | 3:+55237184 | None:intergenic |
| TGTTGTTGGTGAAACTGCTG+TGG | 0.630588 | 3:-55236570 | MsG0380014467.01.T01:CDS |
| GAACATAGCTTATACAAGCA+GGG | 0.631384 | 3:+55236780 | None:intergenic |
| TGGTTTGCGAGAAGGAGGAG+GGG | 0.643319 | 3:+55237213 | None:intergenic |
| AGACATCCTAAGATTGGTGA+TGG | 0.645064 | 3:-55236482 | MsG0380014467.01.T01:CDS |
| ATACTTCATATATTTCTGAG+TGG | 0.645918 | 3:-55236268 | MsG0380014467.01.T01:CDS |
| TAAGGTTGAAAATGGTGTTG+AGG | 0.651117 | 3:-55237059 | MsG0380014467.01.T01:CDS |
| CTGGTGCTAAGATTGGTAGA+GGG | 0.695256 | 3:-55236619 | MsG0380014467.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATAGCTAGATAAAAT+TGG | + | Chr3:55236508-55236527 | None:intergenic | 15.0% |
| !! | AATTTGACAAAATTGAATGA+AGG | + | Chr3:55236325-55236344 | None:intergenic | 20.0% |
| !! | ATTCATGAAAAGATCAAAAA+GGG | + | Chr3:55236620-55236639 | None:intergenic | 20.0% |
| !! | ATTTGACAAAATTGAATGAA+GGG | + | Chr3:55236324-55236343 | None:intergenic | 20.0% |
| !! | TATTCATGAAAAGATCAAAA+AGG | + | Chr3:55236621-55236640 | None:intergenic | 20.0% |
| !!! | ATTTTAGGGAATATTAAGAT+TGG | - | Chr3:55237054-55237073 | MsG0380014467.01.T01:CDS | 20.0% |
| !!! | ATTTTGCATAATGTTACATT+AGG | - | Chr3:55236958-55236977 | MsG0380014467.01.T01:CDS | 20.0% |
| !!! | TTTTCATGATTATGTGAAAA+GGG | + | Chr3:55236355-55236374 | None:intergenic | 20.0% |
| !!! | TTTTTCATGATTATGTGAAA+AGG | + | Chr3:55236356-55236375 | None:intergenic | 20.0% |
| ! | AAAAGATAATCCTATCATGT+TGG | - | Chr3:55237170-55237189 | MsG0380014467.01.T01:CDS | 25.0% |
| ! | ACATGTTCATATTCATGATA+AGG | - | Chr3:55236411-55236430 | MsG0380014467.01.T01:CDS | 25.0% |
| ! | ACTCAGAAATATATGAAGTA+TGG | + | Chr3:55237221-55237240 | None:intergenic | 25.0% |
| ! | ATTCATGATAAGGTTGAAAA+TGG | - | Chr3:55236421-55236440 | MsG0380014467.01.T01:CDS | 25.0% |
| !! | ATACTTCATATATTTCTGAG+TGG | - | Chr3:55237220-55237239 | MsG0380014467.01.T01:CDS | 25.0% |
| !! | CATTGTTTGTTGAATTTCAA+AGG | - | Chr3:55236730-55236749 | MsG0380014467.01.T01:CDS | 25.0% |
| AGCACTCTTGATTCAAAATA+GGG | - | Chr3:55236816-55236835 | MsG0380014467.01.T01:CDS | 30.0% | |
| ATAGGGTAGCACATAAATTA+TGG | - | Chr3:55236773-55236792 | MsG0380014467.01.T01:CDS | 30.0% | |
| TAGCACTCTTGATTCAAAAT+AGG | - | Chr3:55236815-55236834 | MsG0380014467.01.T01:CDS | 30.0% | |
| TGAAAATTCAAGAAGAAGCA+AGG | - | Chr3:55236464-55236483 | MsG0380014467.01.T01:CDS | 30.0% | |
| TTGCATAATGTTACATTAGG+TGG | - | Chr3:55236961-55236980 | MsG0380014467.01.T01:CDS | 30.0% | |
| !! | TAAGATTCCTAGCTTTACTA+TGG | - | Chr3:55237194-55237213 | MsG0380014467.01.T01:CDS | 30.0% |
| AAAACACCATCACCAATCTT+AGG | + | Chr3:55237015-55237034 | None:intergenic | 35.0% | |
| AATGTTACATTAGGTGGAAC+TGG | - | Chr3:55236967-55236986 | MsG0380014467.01.T01:CDS | 35.0% | |
| CATGATTATGTGAAAAGGGT+CGG | + | Chr3:55236351-55236370 | None:intergenic | 35.0% | |
| GAACATAGCTTATACAAGCA+GGG | + | Chr3:55236711-55236730 | None:intergenic | 35.0% | |
| GATTCAAAATAGGGTTTCTG+AGG | - | Chr3:55236825-55236844 | MsG0380014467.01.T01:CDS | 35.0% | |
| GCACATAAATTATGGCTACA+AGG | - | Chr3:55236781-55236800 | MsG0380014467.01.T01:CDS | 35.0% | |
| GGAATCTTATCCAACATGAT+AGG | + | Chr3:55237183-55237202 | None:intergenic | 35.0% | |
| GGGAATATTAAGATTGGTGA+TGG | - | Chr3:55237060-55237079 | MsG0380014467.01.T01:CDS | 35.0% | |
| TGAACATAGCTTATACAAGC+AGG | + | Chr3:55236712-55236731 | None:intergenic | 35.0% | |
| ! | ATTTTGTTGGATCATGCAAC+AGG | - | Chr3:55236892-55236911 | MsG0380014467.01.T01:CDS | 35.0% |
| ! | GTGCTGGAACATGTATTTTA+GGG | - | Chr3:55237040-55237059 | MsG0380014467.01.T01:CDS | 35.0% |
| ! | TAAGGTTGAAAATGGTGTTG+AGG | - | Chr3:55236429-55236448 | MsG0380014467.01.T01:CDS | 35.0% |
| ! | TCATGTTGATGAGGAATGTT+TGG | + | Chr3:55236298-55236317 | None:intergenic | 35.0% |
| !! | TTTAGCTTGTCAAGCTCATA+GGG | - | Chr3:55236756-55236775 | MsG0380014467.01.T01:CDS | 35.0% |
| !! | TTTGCTGTTGATATTCATCC+TGG | - | Chr3:55236850-55236869 | MsG0380014467.01.T01:CDS | 35.0% |
| !!! | ATTGGTGATGGTGTTTTGAT+TGG | - | Chr3:55237018-55237037 | MsG0380014467.01.T01:CDS | 35.0% |
| !!! | TTTTAGCTTGTCAAGCTCAT+AGG | - | Chr3:55236755-55236774 | MsG0380014467.01.T01:CDS | 35.0% |
| AGACATCCTAAGATTGGTGA+TGG | - | Chr3:55237006-55237025 | MsG0380014467.01.T01:CDS | 40.0% | |
| GGTGATAGACATCCTAAGAT+TGG | - | Chr3:55237000-55237019 | MsG0380014467.01.T01:CDS | 40.0% | |
| GTATGGTCCATAGTAAAGCT+AGG | + | Chr3:55237204-55237223 | None:intergenic | 40.0% | |
| TACATTAGGTGGAACTGGTA+AGG | - | Chr3:55236972-55236991 | MsG0380014467.01.T01:CDS | 40.0% | |
| TTTCCAACAGCAGTAGTTCT+TGG | + | Chr3:55237129-55237148 | None:intergenic | 40.0% | |
| TTTCCACCAATCAACTTAGC+TGG | + | Chr3:55237153-55237172 | None:intergenic | 40.0% | |
| ! | ATGGTGTTGAGGTTGATTTC+TGG | - | Chr3:55236440-55236459 | MsG0380014467.01.T01:CDS | 40.0% |
| ! | ATTCATCCTGGTGCTAAGAT+TGG | - | Chr3:55236862-55236881 | MsG0380014467.01.T01:CDS | 40.0% |
| ! | GATTGGTAGAGGGATTTTGT+TGG | - | Chr3:55236879-55236898 | MsG0380014467.01.T01:CDS | 40.0% |
| !! | AATCCAGCTAAGTTGATTGG+TGG | - | Chr3:55237147-55237166 | MsG0380014467.01.T01:CDS | 40.0% |
| !! | ATTGGTGATGGTGCTAAGAT+TGG | - | Chr3:55237072-55237091 | MsG0380014467.01.T01:CDS | 40.0% |
| !! | GGAAATCCAGCTAAGTTGAT+TGG | - | Chr3:55237144-55237163 | MsG0380014467.01.T01:CDS | 40.0% |
| !! | GGTGCTGGAACATGTATTTT+AGG | - | Chr3:55237039-55237058 | MsG0380014467.01.T01:CDS | 40.0% |
| CATGCAACAGGAGTTGTTGT+TGG | - | Chr3:55236904-55236923 | MsG0380014467.01.T01:CDS | 45.0% | |
| GAAGGGTCATCATGTTGATG+AGG | + | Chr3:55236307-55236326 | None:intergenic | 45.0% | |
| ! | CTGGTGCTAAGATTGGTAGA+GGG | - | Chr3:55236869-55236888 | MsG0380014467.01.T01:CDS | 45.0% |
| ! | TGTTGTTGGTGAAACTGCTG+TGG | - | Chr3:55236918-55236937 | MsG0380014467.01.T01:CDS | 45.0% |
| !! | ATGTTTGGTTTGCGAGAAGG+AGG | + | Chr3:55236283-55236302 | None:intergenic | 45.0% |
| !! | GGAATGTTTGGTTTGCGAGA+AGG | + | Chr3:55236286-55236305 | None:intergenic | 45.0% |
| !! | TGCTGGTTCTGTTGTGTTGA+AGG | - | Chr3:55237095-55237114 | MsG0380014467.01.T01:CDS | 45.0% |
| !!! | GATGGTGTTTTGATTGGTGC+TGG | - | Chr3:55237024-55237043 | MsG0380014467.01.T01:CDS | 45.0% |
| CCAACAGCAGTAGTTCTTGG+TGG | + | Chr3:55237126-55237145 | None:intergenic | 50.0% | |
| CCACCAAGAACTACTGCTGT+TGG | - | Chr3:55237123-55237142 | MsG0380014467.01.T01:CDS | 50.0% | |
| CCTCTACCAATCTTAGCACC+AGG | + | Chr3:55236871-55236890 | None:intergenic | 50.0% | |
| GGTGGAACTGGTAAGGTTTG+TGG | - | Chr3:55236979-55236998 | MsG0380014467.01.T01:CDS | 50.0% | |
| ! | CCTGGTGCTAAGATTGGTAG+AGG | - | Chr3:55236868-55236887 | MsG0380014467.01.T01:CDS | 50.0% |
| ! | GATGGTGCTAAGATTGGTGC+TGG | - | Chr3:55237078-55237097 | MsG0380014467.01.T01:CDS | 50.0% |
| ! | GTTGGTGAAACTGCTGTGGT+TGG | - | Chr3:55236922-55236941 | MsG0380014467.01.T01:CDS | 50.0% |
| !! | TTGGTTTGCGAGAAGGAGGA+GGG | + | Chr3:55236279-55236298 | None:intergenic | 50.0% |
| !! | TTTGGTTTGCGAGAAGGAGG+AGG | + | Chr3:55236280-55236299 | None:intergenic | 50.0% |
| ! | TGGTTTGCGAGAAGGAGGAG+GGG | + | Chr3:55236278-55236297 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 55236250 | 55237260 | 55236250 | ID=MsG0380014467.01;Name=MsG0380014467.01 |
| Chr3 | mRNA | 55236250 | 55237260 | 55236250 | ID=MsG0380014467.01.T01;Parent=MsG0380014467.01;Name=MsG0380014467.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|336 |
| Chr3 | exon | 55236250 | 55237260 | 55236250 | ID=MsG0380014467.01.T01:exon:17167;Parent=MsG0380014467.01.T01 |
| Chr3 | CDS | 55236250 | 55237260 | 55236250 | ID=MsG0380014467.01.T01:cds;Parent=MsG0380014467.01.T01 |
| Gene Sequence |
| Protein sequence |
